SitesBLAST
Comparing SMa2137 SMa2137 dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
42% identity, 98% coverage: 7:323/324 of query aligns to 1:321/334 of 5aovA
- active site: L100 (= L106), R241 (= R243), D265 (= D267), E270 (= E272), H288 (= H290)
- binding glyoxylic acid: M52 (≠ T58), L53 (≠ V59), L53 (≠ V59), Y74 (≠ F80), A75 (≠ G81), V76 (= V82), G77 (= G83), R241 (= R243), H288 (= H290)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V82), T104 (= T110), F158 (≠ M160), G159 (= G161), R160 (= R162), I161 (= I163), S180 (≠ D183), R181 (≠ S184), A211 (≠ H213), V212 (≠ C214), P213 (= P215), T218 (≠ N220), I239 (≠ T241), A240 (= A242), R241 (= R243), H288 (= H290), G290 (= G292)
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
42% identity, 98% coverage: 8:323/324 of query aligns to 1:320/332 of 6biiA
- active site: L99 (= L106), R240 (= R243), D264 (= D267), E269 (= E272), H287 (= H290)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (= V82), T103 (= T110), G156 (= G159), F157 (≠ M160), G158 (= G161), R159 (= R162), I160 (= I163), A179 (vs. gap), R180 (≠ D183), S181 (= S184), K183 (≠ S186), V211 (≠ C214), P212 (= P215), E216 (= E219), T217 (≠ N220), V238 (≠ T241), A239 (= A242), R240 (= R243), D264 (= D267), H287 (= H290), G289 (= G292)
O58320 Glyoxylate reductase; EC 1.1.1.26 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
43% identity, 98% coverage: 7:323/324 of query aligns to 1:321/334 of O58320
2dbqA Crystal structure of glyoxylate reductase (ph0597) from pyrococcus horikoshii ot3, complexed with NADP (i41) (see paper)
43% identity, 98% coverage: 7:323/324 of query aligns to 1:321/333 of 2dbqA
- active site: L100 (= L106), R241 (= R243), D265 (= D267), E270 (= E272), H288 (= H290)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V82), T104 (= T110), L158 (≠ M160), G159 (= G161), R160 (= R162), I161 (= I163), S180 (≠ D183), R181 (≠ S184), T182 (≠ H185), A211 (≠ H213), V212 (≠ C214), P213 (= P215), T218 (≠ N220), I239 (≠ T241), A240 (= A242), R241 (= R243), D265 (= D267), H288 (= H290), G290 (= G292)
Q9UBQ7 Glyoxylate reductase/hydroxypyruvate reductase; EC 1.1.1.79; EC 1.1.1.81 from Homo sapiens (Human) (see paper)
37% identity, 99% coverage: 1:321/324 of query aligns to 1:324/328 of Q9UBQ7
2gcgA Ternary crystal structure of human glyoxylate reductase/hydroxypyruvate reductase (see paper)
37% identity, 96% coverage: 10:321/324 of query aligns to 4:320/324 of 2gcgA
- active site: L103 (= L106), R241 (= R243), D265 (= D267), E270 (= E272), H289 (= H290)
- binding (2r)-2,3-dihydroxypropanoic acid: L55 (≠ V59), S78 (≠ G81), V79 (= V82), G80 (= G83), R241 (= R243), H289 (= H290)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V79 (= V82), T107 (= T110), G156 (= G159), G158 (= G161), I160 (= I163), G180 (≠ S184), R181 (≠ H185), R184 (≠ A188), C212 (= C214), S213 (≠ P215), T218 (≠ N220), I239 (≠ T241), R241 (= R243), D265 (= D267), H289 (= H290), G291 (= G292)
6ih6A Phosphite dehydrogenase mutant i151r/p176r/m207a from ralstonia sp. 4506 in complex with non-natural cofactor nicotinamide cytosine dinucleotide
36% identity, 98% coverage: 7:323/324 of query aligns to 1:326/330 of 6ih6A
- binding [[(2S,3S,4R,5S)-5-(3-aminocarbonylpyridin-1-ium-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2S,3S,4R,5S)-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate: T104 (= T110), R151 (≠ I158), G154 (= G161), A155 (≠ R162), V156 (≠ I163), D175 (= D183), A207 (≠ H213), V208 (≠ C214), P209 (= P215), T214 (≠ N220), A235 (≠ T241), C236 (≠ A242), R237 (= R243)
5v6qB Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and malonate (see paper)
37% identity, 95% coverage: 8:315/324 of query aligns to 3:302/319 of 5v6qB
- active site: L96 (= L106), R230 (= R243), D254 (= D267), E259 (= E272), H277 (= H290)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V72 (= V82), V100 (≠ T110), F148 (≠ I158), L150 (≠ M160), G151 (= G161), R152 (= R162), I153 (= I163), T172 (≠ D183), R173 (≠ S184), V201 (≠ C214), P202 (= P215), S206 (≠ E219), T207 (≠ N220), V228 (≠ T241), G229 (≠ A242), R230 (= R243), H277 (= H290), A279 (≠ G292)
5v7nA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and 2-keto-d-gluconic acid (see paper)
37% identity, 95% coverage: 8:315/324 of query aligns to 2:301/319 of 5v7nA
- active site: L95 (= L106), R229 (= R243), D253 (= D267), E258 (= E272), H276 (= H290)
- binding 2-keto-D-gluconic acid: G70 (= G81), V71 (= V82), G72 (= G83), R229 (= R243), H276 (= H290), S279 (= S293), R285 (= R299)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V71 (= V82), V99 (≠ T110), L149 (≠ M160), G150 (= G161), R151 (= R162), I152 (= I163), T171 (≠ D183), R172 (≠ S184), V200 (≠ C214), P201 (= P215), S205 (≠ E219), T206 (≠ N220), V227 (≠ T241), G228 (≠ A242), R229 (= R243), H276 (= H290), A278 (≠ G292)
5j23A Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with 2'-phospho-adp-ribose (see paper)
37% identity, 95% coverage: 8:315/324 of query aligns to 1:300/318 of 5j23A
- active site: L94 (= L106), R228 (= R243), D252 (= D267), E257 (= E272), H275 (= H290)
- binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4r,5r)-3,4,5-trihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: V70 (= V82), L148 (≠ M160), G149 (= G161), R150 (= R162), I151 (= I163), T170 (≠ D183), R171 (≠ S184), P200 (= P215), S204 (≠ E219), T205 (≠ N220), R228 (= R243)
5v7gA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADPH and oxalate (see paper)
37% identity, 95% coverage: 8:315/324 of query aligns to 1:300/317 of 5v7gA
- active site: L94 (= L106), R228 (= R243), D252 (= D267), E257 (= E272), H275 (= H290)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V70 (= V82), V98 (≠ T110), F146 (≠ I158), L148 (≠ M160), G149 (= G161), R150 (= R162), I151 (= I163), T170 (≠ D183), R171 (≠ S184), V199 (≠ C214), P200 (= P215), S204 (≠ E219), T205 (≠ N220), V226 (≠ T241), G227 (≠ A242), R228 (= R243), H275 (= H290), A277 (≠ G292)
- binding oxalate ion: G69 (= G81), V70 (= V82), G71 (= G83), R228 (= R243), H275 (= H290)
3bazA Structure of hydroxyphenylpyruvate reductase from coleus blumei in complex with NADP+ (see paper)
37% identity, 90% coverage: 25:317/324 of query aligns to 14:304/311 of 3bazA
- active site: L98 (= L106), R230 (= R243), A251 (= A264), D254 (= D267), E259 (= E272), H277 (= H290)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V74 (= V82), G149 (= G159), L150 (≠ M160), G151 (= G161), R152 (= R162), I153 (= I163), S172 (≠ D183), R173 (≠ S184), S174 (≠ H185), C201 (= C214), P202 (= P215), T207 (≠ N220), I228 (≠ T241), G229 (≠ A242), R230 (= R243), D254 (= D267), H277 (= H290), G279 (= G292)
Q65CJ7 Hydroxyphenylpyruvate reductase; HPPR; EC 1.1.1.237 from Plectranthus scutellarioides (Coleus) (Solenostemon scutellarioides) (see paper)
37% identity, 90% coverage: 25:317/324 of query aligns to 16:306/313 of Q65CJ7
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
39% identity, 87% coverage: 41:323/324 of query aligns to 32:311/525 of 3ddnB
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
39% identity, 87% coverage: 41:323/324 of query aligns to 31:310/526 of 3dc2A
Sites not aligning to the query:
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
32% identity, 87% coverage: 38:318/324 of query aligns to 34:311/533 of O43175
- T78 (≠ V82) binding
- R135 (≠ P142) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (= RI 162:163) binding
- D175 (= D183) binding
- T207 (≠ C214) binding
- CAR 234:236 (≠ TAR 241:243) binding
- D260 (= D267) binding
- V261 (= V268) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HLGS 290:293) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
33% identity, 83% coverage: 38:306/324 of query aligns to 28:293/297 of 6rj3A
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
33% identity, 83% coverage: 38:306/324 of query aligns to 29:294/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ I158), G147 (= G159), L148 (≠ M160), G149 (= G161), R150 (= R162), I151 (= I163), G152 (= G164), D170 (= D183), H201 (= H213), T202 (≠ C214), P203 (= P215)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
33% identity, 83% coverage: 38:306/324 of query aligns to 29:294/302 of 6rihA
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
33% identity, 83% coverage: 38:306/324 of query aligns to 29:294/303 of 6plgA
Query Sequence
>SMa2137 SMa2137 dehydrogenase
MRRDRIVKPKVIVTRRWPTEVEDRLTAEFDTRLNETDQPYDRRELRAALEEADAVLPTVT
DKISADMLEGGIRAKILGNFGVGFNHIDTAAATKVGLVVTNTPGVLTDATADLAMTLLLM
CARRAGEGERELRAGKWTGWRPTHLCGSHVTGKTVGIIGMGRIGQAVARRCHFGFGMDVV
FFDSHSIAGLDVPARQLPSVDDVLATADFVSLHCPGGGENYHLIDDDRLACMKWSAFLIN
TARGDVVDEHALVRALETRRIAGAGLDVFEGEPRVPGRLAERQDVVLLPHLGSATKETRV
AMGMRVIENLKAFFSGRSPPDAVC
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory