SitesBLAST

O35826 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Rattus norvegicus (Rat) (see paper)
25% identity, 53% coverage: 135:349/408 of query aligns to 438:669/722 of O35826

query
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O35826

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Sites not aligning to the query:

49 H→A: Does not affect kinase activity; almost complete loss of epimerase activity; does not interfere with enzyme oligomerization.
110 H→A: Does not affect kinase activity; almost complete loss of epimerase activity; partial reduction of the dimerization process.
132 H→A: Does not affect kinase activity; almost complete loss of epimerase activity; partial reduction of the dimerization process.
155 H→A: Does not affect kinase activity; almost complete loss of epimerase activity; strong reduction of the dimerization process.
157 H→A: Does not affect kinase activity; almost complete loss of epimerase activity; strong reduction of the dimerization process.
413 mutation D->K,N: Does not affect epimerase activity; does not affect feedback inhibition by CMP-Neu5Ac; almost complete loss of kinase activity; does not interfere with oligomerization.
420 R→M: Does not affect epimerase activity; does not affect feedback inhibition by CMP-Neu5Ac; almost complete loss of kinase activity; does not interfere with oligomerization.

2yhwA High-resolution crystal structures of n-acetylmannosamine kinase: insights about substrate specificity, activity and inhibitor modelling. (see paper)
25% identity, 47% coverage: 159:349/408 of query aligns to 61:261/309 of 2yhwA

query
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2yhwA

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binding calcium ion: N112 (≠ D208), N115 (= N211), G144 (≠ C240), A161 (≠ G257)
binding zinc ion: H165 (= H261), C175 (= C271), C177 (= C273), C182 (= C278)

2yi1A Crystal structure of n-acetylmannosamine kinase in complex with n- acetyl mannosamine 6-phosphate and adp. (see paper)
24% identity, 47% coverage: 159:349/408 of query aligns to 61:260/308 of 2yi1A

query
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2yi1A

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binding adenosine-5'-diphosphate: T140 (≠ V236), G189 (≠ E285), L216 (≠ A305)
binding 2-acetamido-2-deoxy-6-O-phosphono-alpha-D-mannopyranose: G71 (≠ P169), G72 (= G170), R73 (≠ V171), S84 (= S182), T85 (≠ N183), L87 (≠ Y185), N112 (≠ D208), D113 (= D209), G139 (= G235), T140 (≠ V236), G141 (= G237), I142 (≠ V238), E162 (≠ K258), H165 (= H261), E184 (≠ M280)
binding calcium ion: N112 (≠ D208), N115 (= N211), G144 (≠ C240), A161 (≠ G257)
binding zinc ion: H165 (= H261), C175 (= C271), C177 (= C273), C182 (= C278)

Sites not aligning to the query:

2yhyA Structure of n-acetylmannosamine kinase in complex with n- acetylmannosamine and adp (see paper)
24% identity, 47% coverage: 159:349/408 of query aligns to 61:260/308 of 2yhyA

query
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2yhyA

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binding adenosine-5'-diphosphate: T140 (≠ V236), G189 (≠ E285), L216 (≠ A305)
binding calcium ion: N112 (≠ D208), N115 (= N211), G144 (≠ C240), A161 (≠ G257)
binding zinc ion: H165 (= H261), C175 (= C271), C177 (= C273), C182 (= C278)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 11, 12, 13, 14, 16, 261

Q9Y223 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Homo sapiens (Human) (see 16 papers)
24% identity, 47% coverage: 159:349/408 of query aligns to 465:669/722 of Q9Y223

query
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Q9Y223

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I472 (= I166) to T: in NM; results in decreased epimerase activity corresponding to 50% of the wild-type; severely decreased kinase activity corresponding to less than 10% of wild-type activity
D517 (= D209) active site
N519 (= N211) to S: in NM; decreased epimerase activity; decreased kinase activity; dbSNP:rs1554658910
A524 (= A216) to V: in NM; results in decreased epimerase activity corresponding to less than 10% of wild-type activity; decreased kinase activity; impaired homohexamers formation; dbSNP:rs764698870
F528 (= F220) to C: in NM; retains 70% of wild-type epimerase; decreased kinase activity; dbSNP:rs986773986
V572 (≠ H264) to L: in NM; retains 70-80% of wild-type epimerase activity; severely decreased kinase activity corresponding to less than 10% of wild-type activity; does not affect homohexamers formation; dbSNP:rs121908632
G576 (= G268) to E: in NM; decreased epimerase activity; decreased kinase activity; dbSNP:rs121908625
I587 (≠ L279) to T: in NM; decreased epimerase activity; decreased kinase activity; dbSNP:rs748949603
A630 (= A310) to T: in NM; decreased kinase activity; does not affect homohexamers formation; dbSNP:rs1382191649
A631 (≠ I311) to T: in NM; retains 80% of wild-type epimerase activity; retains 75% of wild-type kinase activity; dbSNP:rs121908626; to V: in NM; retains 70% of wild-type epimerase activity; decreased kinase activity; does not affect homohexamers formation; dbSNP:rs62541771

Sites not aligning to the query:

13 C → S: in NM; results in decreased epimerase activity corresponding to 20% of wild-type activity; no effect on kinase activity; dbSNP:rs1209266607
132 H → Q: in NM; results in decreased epimerase activity corresponding to less than 10% of wild-type activity; impaired homohexamers formation
176 D → V: in NM; results in decreased epimerase activity corresponding to 20% of wild-type activity; no effect on kinase activity; impaired homohexamers formation; dbSNP:rs139425890
177 R → C: in NM; results in decreased epimerase activity corresponding to less than 20% of wild-type activity; no effect on kinase activity; impaired homohexamers formation; dbSNP:rs539332585
200 I → F: in NM; unknown pathological significance; retains 90% of wild-type epimerase activity; retains 75% of wild-type kinase activity; dbSNP:rs369328625
206 G → S: in NM; moderate phenotype with unusual involvement of quadriceps; dbSNP:rs766266918
263 R → L: in SIALURIA; strong reduction of feedback inhibition by CMP-Neu5Ac; dbSNP:rs121908623
266 R → Q: in SIALURIA; abolishes feedback inhibition by CMP-Neu5Ac; dbSNP:rs121908622; R → W: in sialuria; dbSNP:rs121908621
303 C → V: in NM; retains 80% of wild-type epimerase activity; decreased kinase activity corresponding to 60% of wild-type; requires 2 nucleotide substitutions; dbSNP:rs121908633
331 V → A: in NM; results in decreased epimerase activity corresponding to 20% of wild-type activity; no effect on kinase activity; impaired homohexamers formation
378 D → Y: in NM; results in decreased epimerase activity corresponding to 10-30% of wild-type activity; decreased kinase activity; impaired homohexamers formation; dbSNP:rs199877522
708 G → S: in NM; decreased epimerase activity; severely decreased kinase activity; dbSNP:rs1554657922

3vovB Crystal structure of rok hexokinase from thermus thermophilus (see paper)
33% identity, 44% coverage: 164:342/408 of query aligns to 61:236/298 of 3vovB

query
sites
3vovB

I

I

G

G

I

L

A

G

M

T

P

P

G

G

V

P

I

L

D

D

N

F

Q

R

R

R

A

G

V

V

C

-

I

I

R

R

S

P

N

N

R

I

Y

P

G

G

W

V

D

Q

N

D

V

F

P

P

L

I

G

R

D

R

L

I

V

L

A

E

S

E

R

A

I

T

G

G

V

R

P

P

V

V

W

F

L

L

E

E

D

N

D

D

T

A

N

N

A

A

Y

A

A

A

I

L

A

A

Q

E

Q

H

L

H

F

L

G

G

L

A

G

A

R

Q

H

G

Y

E

K

E

T

S

M

S

G

L

V

Y

L

L

A

T

I

V

G

S

V

T

G

G

V

I

A

G

C

G

S

G

L

V

V

V

L

L

D

G

G

G

K

R

L

V

Y

L

R

R

G

G

A

E

H

R

G

G

A

Q

A

G

G

G

K

E

F

L

G

G

H
|
H

F

L

P

T

H

L

M

L

E

P

G

G

R

P

P

A

C
|
C

E

G

C
|
C

G

G

K

L

R

E

G

G

C
|
C

L

L

M

E

S

A

Y

L

F

A

S

A

E

G

P

R

A

A

M

L

L

E

Q

R

T

D

W

A

H

T

E

Y

R

A

S

F

G

Q

R

R

P

P

E

-

T

V

D

D

G

T

R

R

A

-

E

E

M

L

V

F

A

R

A

L

I

F

A

Q

A

A

G

G

D

D

K

P

A

K

A

A

H

E

S

R

V

L

M

V

R

L

E

Q

A

A

G

A

E

R

T

Y

L

V

G

G

R

I

H

G

L

L

A

A

G

S

L

L

M

V

N

K

V

A

I

F

D

D

P

P

binding zinc ion: H157 (= H261), C167 (= C271), C169 (= C273), C174 (= C278)

7p7wBBB Ubiquitin-like protein SMT3,N-acetyl-D-glucosamine kinase
26% identity, 62% coverage: 97:349/408 of query aligns to 1:258/306 of 7p7wBBB

query
sites
7p7wBBB

Y

F

A

Q

G

G

G

T

V

M

A

Y

L

Y

G

G

F

F

K

D

L

I

M

G

A

G

G

T

S

K

V

I

E

E

C

F

V

G

V

A

T

F

D

D

L

A

E

D

T

L

T

V

P

R

L

V

A

A

S

R

L

E

R

R

L

V

P

A

L

T

P

P

A

T

H

-

D

-

P

-

D

-

T

-

I

-

A

-

E

E

A

S

L

Y

A

A

A

F

A

L

P

D

K

A

L

I

V

V

A

T

L

L

A

V

N

N

R

N

P

A

A

D

A

A

R

E

L

F

A

G

-

V

-

K

-

G

G

T

I

V

G

G

I

I

A

G

M

I

P

P

G

G

V

I

I

A

D

D

N

V

Q

E

R

T

A

G

V

K

C

L

I

L

R

T

S

S

N

N

R

I

Y

P

G

A

W

A

D

M

N

G

V

H

P

T

L

L

G

Q

D

R

L

D

V

L

A

E

S

E

R

R

I

L

G

Q

V

R

P

P

V

V

W

K

L

I

E

E

D

N

D

D

T

A

N

N

A

C

Y

F

A

A

I

L

A

S

Q

E

Q

A

L

W

F

D

G

E

L

D

G

L

R

R

H

G

Y

E

K

P

T

S

M

V

G

L

V

G

L

L

A

I

I

L

G
|
G

V
x
T

G

G

V

V

A

G

C

G

S

G

L

L

V

I

L

F

D

N

G

G

K

K

L

V

Y

H

R

S

G

G

A

R

H

A

G

N

A

I

A

A

G

G

K

E

F

I

G

G

H
|
H

-

T

-

R

F

L

P

P

H

Y

-

D

-

A

-

L

-

K

-

L

-

G

M

M

E

E

G

N

G

A

-

P

-

I

R

F

P

P

C
|
C

E

G

C
|
C

G

K

K

N

R

S

G

G

C
|
C

L

I

M

D

S

N

Y

Y

F

L

S

S

E
x
G

P

R

A

G

M

F

L
x
E

Q

Q

T

L

W

Y

-

D

H

H

E

Y

R

F

S

S

G

-

R

-

P

-

E

E

T

K

D

L

G

S

R

A

A

P

E

E

M

I

V

I

A

A

A

H

I

Y

A

E

A

Q

G

G

D

E

K

R

A

R

A

A

H

V

S

Q

V

H

M

V

R

E

E

R

A

F

G

M

E

E

T

L

L

L

G

A

R

I

H

C

L

L

A

A

G

N

L

I

M

F

N

T

V

C

I

L

D

D

P

P

E

H

V

V

I

V

V

V

V

L

G

G

G
|
G

binding adenosine-5'-diphosphate: G135 (= G235), T136 (≠ V236), G196 (≠ E285), E200 (≠ L289), G258 (= G349)
binding zinc ion: H161 (= H261), C182 (= C271), C184 (= C273), C189 (= C278)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 259, 262

7p9pAAA Ubiquitin-like protein SMT3,N-acetyl-D-glucosamine kinase
27% identity, 62% coverage: 99:349/408 of query aligns to 1:256/304 of 7p9pAAA

query
sites
7p9pAAA

G

G

G

T

V

M

A

Y

L

Y

G

G

F

F

K

D

L

I

M

G

A
x
G

G
x
T

S
x
K

V

I

E

E

C

F

V

G

V

A

T

F

D

D

L

A

E

D

T

L

T

V

P

R

L

V

A

A

S

R

L

E

R

R

L

V

P

A

L

T

P

P

A

T

H

-

D

-

P

-

D

-

T

-

I

-

A

-

E

E

A

S

L

Y

A

A

A

F

A

L

P

D

K

A

L

I

V

V

A

T

L

L

A

V

N

N

R

N

P

A

A

D

A

A

R

E

L

F

A

G

-

V

-

K

-

G

G

T

I

V

G

G

I

I

A

G

M

I

P

P

G

G

V

I

I

A

D

D

N

V

Q

E

R

T

A

G

V

K

C

L

I

L