SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SMa2313 FitnessBrowser__Smeli:SMa2313 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q7JK39 Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; D-xylose 1-dehydrogenase; D-xylose-NADP dehydrogenase; Dimeric dihydrodiol dehydrogenase; Jmo2DD; EC 1.3.1.20; EC 1.1.1.179 from Macaca fuscata fuscata (Japanese macaque) (see paper)
27% identity, 50% coverage: 7:347/677 of query aligns to 3:334/334 of Q7JK39

query
sites
Q7JK39

I

L

R

R

W

W

G

G

I

I

L

V

G

S

P

V

G

G

N

L

I

I

A

S

K

S

D

D

F

F

F

T

A

A

-

V

-

L

G

Q

A

T

L

L

Q

P

S

R

A

S

N

E

G

H

K

Q

V

V

A

A

I

V

G

A

A

A

R

R

N

D

P

L

A

S

K

R

S

A

-

K

-

E

-

F

G

A

L

Q

A

K

E

H

D

D

F

I

P

P

G

K

A

A

R

-

I

-

V

Y

D

G

G

S

Y

Y

D

E

A

E

L

L

I

A

D

K

D

D

P

P

G

N

I

V

D

E

A

V

V

A

Y

Y

I

V

A

G

T

T

V

Q

H

H

P

P

L

Q

H
|
H

A

K

E

A

W

A

A

V

I

M

K

L

A

C

A

L

E

A

K

A

G

G

K

K

H

A

V

V

L

L

C

C

E

E

K

K

P

P

M

M

G

G

L

V

S

N

T

A

A

A

E

E

A

V

D

R

A

E

M

M

F

V

E

T

A

E

A

A

R

R

K

S

A

R

G

G

T

L

F

F

M

L

A

M

E

E

A

A

Y

I

M

W

Y

T

R

R

L

F

H

F

P

P

L

A

T

S

A

E

R

A

I

L

V

R

E

S

L

V

V

L

K

A

N

Q

G

G

M

T

V

L

G

G

D

D

V

L

R

R

K

V

I

A

Q

R

S

A

S

E

F

F

G

G

-

K

-

N

F

L

A

T

K

H

L

V

P

P

F

-

D

-

E

-

G

-

H

-

R

R

L

A

F

V

S

D

N

W

E

A

M

Q

A

A

G

G

G

A

I

L

L

L

D

D

V

L

G

G

G

I

Y
|
Y

T

C

T

V

S

Q

M

F

A

I

R

S

L

M

I

V

A

-

G

-

I

-

G

-

T

-

S

-

S

F

G

G

V

G

M

Q

E

K

P

P

A

E

E

K

V

I

T

S

A

V

L

M

G

G

H

R

L

R

G

H

R

E

T

T

G

G

V

V

D

D

E

D

W

T

T

V

S

T

A

V

L

L

S

Q

F

Y

P

P

N

G

G

E

I

V

I

H

A

G

E

S

L

F

S

T

C

C

S

S

V

I

S

T

L

A

E

Q

Q

L

E

S

N

N

V

T

L

A

R

S

I

V

L

S

G

G

T

T

K

K

G

G

R

M

I

A

E

Q

V

L

D

L

Q

N

F

-

W

-

F

-

A

-

G

-

K

-

P

P

G

C

G

W

T

C

S

P

I

T

I

E

R

L

I

V

V

V

H

K

A

G

D

E

G

H

R

K

Q

E

E

F

E

L

V

L

P

P

L

P

V

V

E

-

P

P

R

K

H

N

L

C

-

N

-

F

-

D

-

N

-

G

-

A

-

G

Y

M

S

S

F

Y

E

E

V

A

E

K

A

H

A

V

G

R

D

E

A

C

I

L

R

R

A

K

G

G

R

L

T

K

E

E

F

S

A

P

Y

V

P

I

G

P

M

L

S

V

R

E

A

S

D

E

T

L

L

L

G

A

N

D

L

-

R

-

V

I

M

L

D

E

K

E

W

V

R

R

A

R

A

A

I

I

G

G

L

V

E

T

Y

F

E

P

G

Q

E

D

K

K

H

H

H79 (= H80) mutation to E: Decrease in K(d) and K(m) value for NADPH. Elimination of the fluorescence-energy transfer and enhancement of NADPH fluorescence by the binary complex formation. Potent inhibition of the dehydrogenase activity by high ionic strength.
Y180 (= Y184) mutation to F: Significant loss of activity. No effect on the high affinity for NADPH, fluorescence-energy transfer and enhancement of NADPH fluorescence by the binary complex formation.

Q9TQS6 Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; Cmo2DD; D-xylose 1-dehydrogenase; D-xylose-NADP dehydrogenase; Dimeric dihydrodiol dehydrogenase; EC 1.3.1.20; EC 1.1.1.179 from Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) (see paper)
27% identity, 50% coverage: 7:347/677 of query aligns to 3:334/334 of Q9TQS6

query
sites
Q9TQS6

I

L

R

R

W

W

G

G

I

I

L

V

G

S

P

V

G

G

N

L

I

I

A

S

K

S

D

D

F

F

F

T

A

A

-

V

-

L

G

Q

A

T

L

L

Q

P

S

R

A

S

N

E

G

H

K

Q

V

V

A

A

I

V

G

A

A

A

R

R

N

D

P

L

A

S

K

R

S

A

-

K

-

E

-

F

G

A

L

Q

A

K

E

H

D

D

F

I

P

P

G

K

A

A

R

-

I

-

V

Y

D

G

G

S

Y

Y

D

E

A

E

L

L

I

A

D

K

D

D

P

P

G

N

I

V

D

E

A

V

V

A

Y

Y

I

V

A

G

T

T

V

Q

H

H

P

P

L

Q

H

H

A

K

E

A

W

A

A

V

I

M

K

L

A

C

A

L

E

A

K

A

G

G

K

K

H

A

V

V

L

L

C

C

E

E

K

K

P

P

M

M

G

G

L

V

S

N

T

A

A

A

E

E

A

V

D

R

A

E

M

M

F

V

E

T

A

E

A

A

R

R

K

S

A

R

G

G

T

L

F

F

M

L

A

M

E

E

A

A

Y

I

M

W

Y

T

R

R

L

F

H

F

P

P

L

A

T

S

A

E

R

A

I

L

V

R

E

S

L

V

V

L

K

A

N

Q

G

G

M

T

V

L

G

G

D

D

V

L

R
|
R

K

V

I

A

Q

R

S

A

S

E

F

F

G

G

-

K

-

N

F

L

A

T

K

H

L

V

P

P

F

-

D

-

E

-

G

-

H

-

R

R

L

A

F

V

S

D

N

W

E

A

M

Q

A

A

G

G

G

A

I

L

L

L

D

D

V

L

G

G

G

I

Y

Y

T

C

T

V

S

Q

M

F

A

I

R

S

L

M

I

V

A

-

G

-

I

-

G

-

T

-

S

-

S

F

G

G

V

G

M

Q

E

K

P

P

A

E

E

K

V

I

T

S

A

V

L

M

G

G

H
x
R

L

R

G

H

R

E

T

T

G

G

V

V

D

D

E

D

W

T

T

V

S

T

A

V

L

L

S

Q

F

Y

P

P

N

G

G

E

I

V

I

H

A

G

E

S

L

F

S

T

C

C

S

S

V

I

S

T

L

A

E

Q

Q

L

E

S

N

N

V

T

L

A

R

S

I

V

L

S

G

G

T

T

K

K

G

G

R

M

I

A

E

Q

V

L

D

L

Q

N

F

-

W

-

F

-

A

-

G

-

K

-

P

P

G

C

G

W

T

C

S

P

I

T

I

E

R

L

I

V

V

V

H

K

A

G

D

E

G

H

R

K

Q

E

E

F

E

L

V

L

P

P

L

P

V

V

E

-

P

P

R

K

H

N

L

C

-

N

-

F

-

D

-

N

-

G

-

A

-

G

Y

M

S

S

F

Y

E

E

V

A

E

K

A

H

A

V

G

R

D

E

A

C

I

L

R

R

A

K

G

G

R

L

T

K

E

E

F

S

A

P

Y

V

P

I

G

P

M

L

S

V

R

E

A

S

D

E

T

L

L

L

G

A

N

D

L

-

R

-

V

I

M

L

D

E

K

E

W

V

R

R

A

R

A

A

I

I

G

G

L

V

E

T

Y

F

E

P

G

Q

E

D

K

K

H

H

R148 (= R149) mutation to A: No effect on activity. Reduced activity and exhibits significant temperature sensitivity; when associated with A-202.
R202 (≠ H212) mutation to A: No effect on activity. Reduced activity and exhibits significant temperature sensitivity; when associated with A-148.

2poqX Dimeric dihydrodiol dehydrogenase complexed with inhibitor, isoascorbic acid (see paper)
27% identity, 50% coverage: 7:342/677 of query aligns to 2:328/331 of 2poqX

query
sites
2poqX

I

L

R

R

W

W

G

G

I

I

L

V

G

S

P

V

G

G

N

L

I

I

A

S

K

S

D

D

F

F

F

T

A

A

-

V

-

L

G

Q

A

T

L

L

Q

P

S

R

A

S

N

E

G

H

K

Q

V

V

A

A

I

V

G

A

A

A

R

R

N

D

P

L

A

S

K

R

S

A

-

K

-

E

-

F

G

A

L

Q

A

K

E

H

D

D

F

I

P

P

G

K

A

A

R

-

I

-

V

Y

D

G

G

S

Y

Y

D

E

A

E

L

L

I

A

D

K

D

D

P

P

G

N

I

V

D

E

A

V

V

A

Y

Y

I

V

A

G

T

T

V

Q

H

H

P

P

L

Q

H

H

A

K

E

A

W

A

A

V

I

M

K

L

A

C

A

L

E

A

K

A

G

G

K

K

H

A

V

V

L

L

C

C

E

E

K

K

P

P

M

M

G

G

L

V

S

N

T

A

A

A

E

E

A

V

D

R

A

E

M

M

F

V

E

T

A

E

A

A

R

R

K

S

A

R

G

G

T

L

F

F

M

L

A

M

E

E

A

A

Y

I

M

W

Y

T

R

R

L

F

H

F

P

P

L

A

T

S

A

E

R

A

I

L

V

R

E

S

L

V

V

L

K

A

N

Q

G

G

M

T

V

L

G

G

D

D

V

L

R

R

K

V

I

A

Q

R

S

A

S

E

F

F

G

G

-
x
K

-

N

F

L

A

T

K

H

L

V

P

P

F

-

D

-

E

-

G

-

H

-

R

R

L

A

F

V

S

D

N

W

E

A

M

Q

A

A

G

G

G

A

I

L

L

L

D

D

V

L

G

G

G

I

Y

Y

T

C

T

V

S

Q

M

F

A

I

R

S

L

M

I

V

A

-

G

-

I

-

G

-

T

-

S

-

S

F

G

G

V

G

M

Q

E

K

P

P

A

E

E

K

V

I

T

S

A

V

L

M

G

G

H

R

L

R

G

H

R

E

T

T

G

G

V

V

D

D

E

D

W

T

T

V

S

T

A

V

L

L

S

Q

F

Y

P

P

N

G

G

E

I

V

I

H

A

G

E

S

L

F

S

T

C

C

S

S

V

I

S

T

L

A

E

Q

Q

L

E

S

N

N

V

T

L

A

R

S

I

V

L

S

G

G

T

T

K

K

G

G

R

M

I

A

E

Q

V

L

D

L

Q

N

F

-

W

-

F

-

A

-

G

-

K

-

P

P

G

C

G

W

T

C

S

P

I

T

I

E

R

L

I

V

V

V

H

K

A

G

D

E

G

H

R

K

Q

E

E

F

E

L

V

L

P

P

L

P

V

V

E

-

P

P

R

K

H

N

L

C

-

N

-
x
F

-
x
D

-

N

-

G

-

A

-

G

Y

M

S

S

F

Y

E

E

V

A

E

K

A

H

A

V

G

R

D

E

A

C

I

L

R

R

A

K

G

G

R

L

T

K

E

E

F

S

A

P

Y

V

P

I

G

P

M

L

S

V

R

E

A

S

D

E

T

L

L

L

G

A

N

D

L

-

R

-

V

I

M

L

D

E

K

E

W

V

R

R

A

R

A

A

I

I

G

G

L

V

E

T

Y

F

binding isoascorbic acid: K155 (vs. gap), F278 (vs. gap), D279 (vs. gap)

2o4uX Crystal structure of mammalian dimeric dihydrodiol dehydrogenase (see paper)
27% identity, 50% coverage: 7:342/677 of query aligns to 2:328/331 of 2o4uX

query
sites
2o4uX

I
x
L

R

R

W

W

G

G

I

I

L

V

G
x
S

P
x
V

G

G

N

L

I

I

A

S

K

S

D

D

F

F

F

T

A

A

-

V

-

L

G

Q

A

T

L

L

Q

P

S

R

A

S

N

E

G

H

K

Q

V

V

A

A

I

V

G

A

A
|
A

R
|
R

N

D

P

L

A

S

K
x
R

S

A

-

K

-

E

-

F

G

A

L

Q

A

K

E

H

D

D

F

I

P

P

G

K

A

A

R

-

I

-

V

Y

D

G

G

S

Y
|
Y

D

E

A

E

L

L

I

A

D

K

D

D

P

P

G

N

I

V

D

E

A

V

V

A

Y

Y

I

V

A

G

T

T

V

Q

H

H

P

P

L

Q

H

H

A

K

E

A

W

A

A

V

I

M

K

L

A

C

A

L

E

A

K

A

G

G

K

K

H

A

V

V

L

L

C

C

E

E

K

K

P

P

M

M

G

G

L

V

S
x
N

T

A

A
|
A

E

E

A

V

D

R

A

E

M

M

F

V

E

T

A

E

A

A

R

R

K

S

A

R

G

G

T

L

F

F

M

L

A

M

E

E

A

A

Y

I

M

W

Y

T

R

R

L

F

H

F

P

P

L

A

T

S

A

E

R

A

I

L

V

R

E

S

L

V

V

L

K

A

N

Q

G

G

M

T

V

L

G

G

D
|
D

V

L

R
|
R

K

V

I

A

Q

R

S

A

S

E

F

F

G

G

-
x
K

-

N

F

L

A

T

K

H

L

V

P

P

F

-

D

-

E

-

G

-

H

-

R

R

L

A

F

V

S

D

N

W

E

A

M

Q

A

A

G

G

G

A

I

L

L

L

D

D

V

L

G

G

G

I

Y

Y

T

C

T

V

S

Q

M

F

A

I

R

S

L

M

I

V

A

-

G

-

I

-

G

-

T

-

S

-

S

F

G

G

V

G

M

Q

E

K

P

P

A

E

E

K

V

I

T

S

A

V

L

M

G

G

H

R

L

R

G

H

R

E

T

T

G

G

V

V

D

D

E

D

W

T

T

V

S

T

A

V

L

L

S

Q

F

Y

P

P

N

G

G

E

I

V

I

H

A

G

E

S

L

F

S

T

C

C

S

S

V

I

S

T

L

A

E
x
Q

Q

L

E
x
S

N

N

V
x
T

L

A

R

S

I

V

L

S

G

G

T

T

K

K

G

G

R

M

I

A

E
x
Q

V

L

D

L

Q
x
N

F

-

W

-

F

-

A

-

G

-

K

-

P

P

G

C

G
x
W

T

C

S

P

I

T

I

E

R

L

I

V

V

V

H

K

A

G

D

E

G

H

R

K

Q

E

E

F

E

L

V

L

P

P

L

P

V

V

E

-

P

P

R

K

H

N

L

C

-

N

-

F

-

D

-

N

-

G

-

A

-

G

Y

M

S

S

F

Y

E

E

V

A

E

K

A

H

A

V

G
x
R

D

E

A

C

I

L

R
|
R

A

K

G

G

R

L

T

K

E

E

F

S

A

P

Y

V

P

I

G

P

M

L

S

V

R

E

A

S

D

E

T

L

L

L

G

A

N

D

L

-

R

-

V

I

M

L

D
x
E

K

E

W

V

R

R

A

R

A

A

I

I

G

G

L

V

E

T

Y

F

binding phosphate ion: L2 (≠ I7), S8 (≠ G13), V9 (≠ P14), A35 (= A38), R36 (= R39), R40 (≠ K43), Y57 (= Y59), N101 (≠ S103), A103 (= A105), D145 (= D147), R147 (= R149), K155 (vs. gap), Q232 (≠ E243), S234 (≠ E245), T236 (≠ V247), Q247 (≠ E258), N250 (≠ Q261), W253 (≠ G271), R292 (≠ G304), R296 (= R308), E318 (≠ D332)

Sites not aligning to the query:

binding phosphate ion: 1

2glxA Crystal structure analysis of bacterial 1,5-af reductase (see paper)
29% identity, 44% coverage: 8:307/677 of query aligns to 2:293/332 of 2glxA

query
sites
2glxA

R

R

W

W

G

G

I

L

L

I

G
|
G

P
x
A

G
x
S

N
x
T

I
|
I

A

A

K

R

D

E

F

W

F

V

A

I

G

G

A

A

L

I

Q

R

S

A

A

T

N

G

G

G

K

E

V

V

A

S

I

M

G

M

A
x
S

R
x
T

N

S

P

A

A

E

K
x
R

-

G

S

A

G

A

L

Y

A

A

E

T

D

E

F

N

P

G

G

I

A

G

R

K

I

S

V

V

D

T

G

S

Y

V

D

E

A

E

L

L

I

V

D

G

D

D

P

P

G

D

I

V

D

D

A

A

V

V

Y

Y

I

V

A
x
S

T
|
T

V

T

H
x
N

P

E

L

L

H
|
H

A

R

E

E

W

Q

A

T

I

L

K

A

A

A

A

I

E

R

K

A

G

G

K

K

H

H

V

V

L

L

C

C

E
|
E

K
|
K

P

P

M

L

G

A

L

M

S

T

T

L

A

E

E

D

A

A

D

R

A

E

M

M

F

V

E

V

A

A

R

R

K

E

A

A

G

G

T

V

F

V

M

L

A

G

E

T

A

N

Y

H

M
x
H

Y

L

R

R

L

N

H

A

P

A

L

A

T

H

A

R

R

A

I

M

V

R

E

D

L

A

V

I

K

A

N

E

G

G

M

R

V

I

G

G

D

-

V

-

R

R

K

P

I

I

Q

A

S

A

S

R

F

V

G

F

F

H

A

A

K

V

L

Y

-

L

-

P

P

P

F

H

D

L

E

Q

G

G

H
x
W

R
|
R

L

L

F

E

S

R

N

P

E

E

M

A

A

G

G

G

G

V

I

I

L

L

D

D

V

I

G

T

G

V

Y
x
H

T

D

T

A

S

D

M

T

A

L

R

R

L

F

I

V

A

L

G

N

I

-

G

-

T

-

S

-

G

-

V

-

M

D

E

D

P

P

A

A

E

E

V

A

T

V

A

A

L

I

G

S

H

H

-

S

-

A

-

G

L

M

G

G

R

K

T

E

G

G

V

V

D

E

E

D

W

G

T

V

S

M

A

G

L

V

L

L

S

R

F

F

P

Q

N

S

G

G

I

V

I

I

A

A

E

Q

L

F

S

H

C

D

S

A

V

F

S

T

L

T

E

K

-

F

Q

A

E

E

N

T

V

G

L

F

R

E

I

V

L

H

G

G

T

T

K

E

G

G

R

S

I

L

E

I

V

G

D

R

Q

N

F

V

W

M

F

T

A

Q

G

-

K

K

P

P

G

V

G

G

T

T

S

V

I

T

I

L

R

R

I

-

V

-

H

N

A

A

D

E

G

G

R

-

Q

E

E

S

E

Q

V

L

P

P

L

L

V

-

E

D

P

P

R

A

H

N

L

L

Y
|
Y

S

E

F

T

E

A

V

L

E

A

A

A

A

F

G

H

D

S

A

A

I

I

active site: K93 (= K98), H179 (≠ Y184)
binding acetate ion: K93 (= K98), H179 (≠ Y184)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G13), A8 (≠ P14), S9 (≠ G15), T10 (≠ N16), I11 (= I17), S32 (≠ A38), T33 (≠ R39), R37 (≠ K43), S69 (≠ A74), T70 (= T75), N72 (≠ H77), H75 (= H80), E92 (= E97), K93 (= K98), H121 (≠ M126), W161 (≠ H166), R162 (= R167), Y282 (= Y296)

5a06A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with sorbitol (see paper)
30% identity, 41% coverage: 59:337/677 of query aligns to 62:328/336 of 5a06A

query
sites
5a06A

Y

F

D

D

A

R

L

I

I

I

D

D

D

N

P

P

G

D

I

V

D
|
D

A

I

V

V

Y

Y

I

V

A
x
I

T
|
T

V
x
P

H
x
N

P

S

L
|
L

H
|
H

A

R

E

P

W

F

A

T

I

E

K

R

A

A

E

R

K

A

G

G

K

K

H
|
H

V

V

L

M

C

C

E
|
E

K
|
K

P

P

M

M

G

A

L

N

S

T

T

V

A

A

E

D

A

C

D

E

A

A

M

M

F

I

E

A

A

A

A

C

R

K

K

K

A

A

G

G

T
x
R

F

K

M
x
L

A

M

E

I

A

G

Y

Y

M
x
R

Y

S

R

R

L

F

H

Q

P

A

L

H

T

N

A

I

R

E

I

A

V

I

E

K

L

L

V

V

K

R

N

D

G

G

M

A

V

L

G

G

D

P

V

V

R

R

K

T

I

V

Q

V

S

T

S

D

F

H

G

G

F
|
F

A

T

K

I

L

-

P

-

F

G

D

D

E

P

G

K

H

Q

R
x
W

L
x
R

F

L

S

N

N

R

E

A

M

L

A

A

G

G

-

S

I

L

L

M

D
|
D

V

I

G

G

G

I

Y
|
Y

T

S

T

L

S

N

M

A

A

A

R

R

L

Y

I

L

A

T

G

G

I

-

G

-

T

-

S

-

G

-

V

-

M

E

E

E

P

P

A

V

E

A

V

V

T

N

A

A

L

V

G

E

H

S

L

T

G

D

R

R

T

S

G

D

-

P

-

R

-

F

-

G

-

E

V

V

D

E

E

D

W

I

T

I

S

N

A

F

L

Q

L

L

S

L

F

F

P

P

N

S

G

G

I

A

I

T

A

A

E

N

L

C

S

V

C

S

S

A

V

Y

S

S

L

V

E

N

Q

C

E

-

N

N

V

R

L

Y

R

R

I

V

L

S

G

G

T

P

K

K

G

G

R

W

I

V

E

E

V

I

D

D

Q

P

F

A

W

T

F

S

A
x
Y

G

Q

G

G

K

Q

P

-

G

-

T

-

S

-

I

A

I

M

R

R

I

A

V

Q

H

L

A

G

D

G

G

P

R

P

Q

A

E

P

E

R

V

E

P

P

L

A

V

P

E

Q

P

P

R
x
K

H

N

L

Q

Y

F

S

S

F

A

E

Q

V

L

E

D

A
x
H

A

L

G

S

D
x
E

A

C

I

I

R

L

A

T

G

G

R

R

T
x
E

E

-

F

-

A

-

Y

-

P

P

G

I

M

V

S
x
G

R
x
G

A

D

D

D

T

G

L

L

G

K

N

D

L

L

R

R

V

V

M

I

D

E

K

A

-

I

W

Y

R

R

A

A

active site: K101 (= K98), Y186 (= Y184)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I77 (≠ A74), T78 (= T75), P79 (≠ V76), N80 (≠ H77), L82 (= L79), H83 (= H80), E100 (= E97), K101 (= K98), R129 (≠ M126), W168 (≠ R167), R169 (≠ L168), Y186 (= Y184), Y264 (≠ A265)
binding sorbitol: D72 (= D69), H96 (= H93), K101 (= K98), R122 (≠ T119), R122 (≠ T119), L124 (≠ M121), F160 (= F157), R169 (≠ L168), D182 (= D180), Y186 (= Y184), K287 (≠ R293), H296 (≠ A302), E299 (≠ D305), E306 (≠ T312), G310 (≠ S320), G311 (≠ R321)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 11, 12, 13, 14, 36, 37, 38, 41, 59
binding sorbitol: 2

5a03C Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with xylose (see paper)
30% identity, 41% coverage: 59:337/677 of query aligns to 62:328/336 of 5a03C

query
sites
5a03C

Y

F

D

D

A

R

L

I

I

I

D

D

D

N

P

P

G

D

I

V

D

D

A

I

V

V

Y

Y

I

V

A
x
I

T
|
T

V
x
P

H
x
N

P

S

L
|
L

H
|
H

A

R

E

P

W

F

A

T

I

E

K

R

A

A

E

R

K

A

G

G

K

K

H

H

V

V

L

M

C

C

E
|
E

K
|
K

P

P

M

M

G

A

L

N

S

T

T

V

A

A

E

D

A

C

D

E

A

A

M

M

F

I

E

A

A

A

A

C

R

K

K

K

A

A

G

G

T

R

F

K

M

L

A

M

E

I

A

G

Y

Y

M
x
R

Y

S

R

R

L

F

H

Q

P

A

L

H

T

N

A

I

R

E

I

A

V

I

E

K

L

L

V

V

K

R

N

D

G

G

M

A

V

L

G

G

D

P

V

V

R

R

K

T

I

V

Q

V

S

T

S

D

F

H

G

G

F
|
F

A

T

K

I

L

-

P

-

F

G

D

D

E

P

G

K

H

Q

R
x
W

L
x
R

F

L

S

N

N

R

E

A

M

L

A

A

G

G

-

S

I

L

L

M

D
|
D

V

I

G

G

G

I

Y
|
Y

T

S

T

L

S

N

M

A

A

A

R

R

L

Y

I

L

A

T

G

G

I

-

G

-

T

-

S

-

G

-

V

-

M

E

E

E

P

P

A

V

E

A

V

V

T

N

A

A

L

V

G

E

H

S

L

T

G

D

R

R

T

S

G

D

-

P

-

R

-

F

-

G

-

E

V

V

D

E

E

D

W

I

T

I

S

N

A

F

L

Q

L

L

S

L

F

F

P

P

N

S

G

G

I

A

I

T

A

A

E

N

L

C

S

V

C

S

S

A

V

Y

S

S

L

V

E

N

Q

C

E

-

N

N

V

R

L

Y

R

R

I

V

L

S

G

G

T

P

K

K

G

G

R

W

I

V

E

E

V

I

D

D

Q

P

F

A

W

T

F

S

A
x
Y

G

Q

G

G

K

Q

P

-

G

-

T

-

S

-

I

A

I

M

R

R

I

A

V

Q

H

L

A

G

D

G

G

P

R

P

Q

A

E

P

E

R

V

E

P

P

L

A

V

P

E

Q

P

P

R

K

H

N

L

Q

Y

F

S

S

F

A

E

Q

V

L

E

D

A

H

A

L

G

S

D

E

A

C

I

I

R

L

A

T

G

G

R

R

T

E

E

-

F

-

A

-

Y

-

P

P

G

I

M

V

S

G

R

G

A

D

D

D

T

G

L

L

G

K

N

D

L

L

R

R

V

V

M

I

D

E

K

A

-

I

W

Y

R

R

A

A

active site: K101 (= K98), Y186 (= Y184)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I77 (≠ A74), T78 (= T75), P79 (≠ V76), N80 (≠ H77), L82 (= L79), H83 (= H80), E100 (= E97), K101 (= K98), R129 (≠ M126), W168 (≠ R167), R169 (≠ L168), Y186 (= Y184), Y264 (≠ A265)
binding beta-D-xylopyranose: K101 (= K98), F160 (= F157), R169 (≠ L168), D182 (= D180), Y186 (= Y184)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 10, 11, 12, 13, 14, 36, 37, 38, 41, 59

5a05A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with maltotriose (see paper)
30% identity, 42% coverage: 57:337/677 of query aligns to 59:327/335 of 5a05A

query
sites
5a05A

D

E

G

T

Y

F

D

D

A

R

L

I

I

I

D

D

D

N

P

P

G

D

I

V

D

D

A

I

V

V

Y

Y

I

V

A
x
I

T
|
T

V
x
P

H
x
N

P

S

L
|
L

H
|
H

A

R

E

P

W

F

A

T

I

E

K

R

A

A

E

R

K

A

G

G

K

K

H

H

V

V

L

M

C

C

E
|
E

K
|
K

P

P

M

M

G

A

L

N

S

T

T

V

A

A

E

D

A

C

D

E

A

A

M

M

F

I

E

A

A

A

A

C

R

K

K

K

A

A

G

G

T

R

F

K

M

L

A

M

E

I

A

G

Y

Y

M
x
R

Y

S

R

R

L

F

H

Q

P

A

L

H

T

N

A

I

R

E

I

A

V

I

E

K

L

L

V

V

K

R

N

D

G

G

M

A

V

L

G

G

D

P

V

V

R

R

K

T

I

V

Q

V

S

T

S

D

F

H

G

G

F
|
F

A

T

K

I

L

-

P

-

F

G

D

D

E

P

G

K

H

Q

R
x
W

L
x
R

F

L

S

N

N

R

E

A

M

L

A

A

G

G

-

S

I

L

L

M

D
|
D

V

I

G

G

G

I

Y
|
Y

T

S

T

L

S

N

M

A

A

A

R

R

L

Y

I

L

A

T

G

G

I

-

G

-

T

-

S

-

G

-

V

-

M

E

E

E

P

P

A

V

E

A

V

V

T

N

A

A

L

V

G

E

H

S

L

T

G

D

R

R

T

S

G

D

-

P

-

R

-

F

-

G

-

E

V

V

D

E

E

D

W

I

T

I

S

N

A

F

L

Q

L

L

S

L

F

F

P

P

N

S

G

G

I

A

I

T

A

A

E

N

L

C

S

V

C

S

S

A

V

Y

S

S

L

V

E

N

Q

C

E

-

N

N

V

R

L

Y

R

R

I

V

L

S

G

G

T

P

K

K

G

G

R

W

I

V

E

E

V

I

D

D

Q
x
P

F

A

W

T

F
x
S

A
x
Y

G

Q

G

G

K

Q

P

-

G

-

T

-

S

-

I

A

I

M

R

R

I

A

V

Q

H

L

A

G

D

G

G

P

R

P

Q

A

E

P

E

R

V

E

P

P

L

A

V

P

E

Q

P

P

R

K

H

N

L

Q

Y

F

S

S

F

A

E

Q

V

L

E

D

A

H

A

L

G

S

D

E

A

C

I

I

R

L

A

T

G

G

R

R

T

E

E

-

F

-

A

-

Y

-

P

P

G

I

M

V

S

G

R

G

A

D

D

D

T

G

L

L

G

K

N

D

L

L

R

R

V

V

M

I

D

E

K

A

-

I

W

Y

R

R

A

A

active site: K100 (= K98), Y185 (= Y184)
binding beta-D-glucopyranose: K100 (= K98), F159 (= F157), D181 (= D180), Y185 (= Y184)
binding alpha-D-glucopyranose: P259 (≠ Q261), S262 (≠ F264)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I76 (≠ A74), T77 (= T75), P78 (≠ V76), N79 (≠ H77), L81 (= L79), H82 (= H80), E99 (= E97), K100 (= K98), R128 (≠ M126), W167 (≠ R167), R168 (≠ L168), Y185 (= Y184), Y263 (≠ A265)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 9, 10, 11, 12, 13, 35, 36, 37, 40, 58

5a04A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with glucose (see paper)
30% identity, 42% coverage: 57:337/677 of query aligns to 59:327/335 of 5a04A

query
sites
5a04A

D

E

G

T

Y

F

D

D

A

R

L

I

I

I

D

D

D

N

P

P

G

D

I

V

D

D

A

I

V

V

Y

Y

I

V

A
x
I

T
|
T

V
x
P

H
x
N

P

S

L
|
L

H
|
H

A

R

E

P

W

F

A

T

I

E

K

R

A

A

E

R

K

A

G

G

K

K

H

H

V

V

L

M

C

C

E
|
E

K
|
K

P

P

M

M

G

A

L

N

S

T

T

V

A

A

E

D

A

C

D

E

A

A

M

M

F

I

E

A

A

A

A

C

R

K

K

K

A

A

G

G

T

R

F

K

M

L

A

M

E

I

A

G

Y

Y

M
x
R

Y

S

R

R

L

F

H

Q

P

A

L

H

T

N

A

I

R

E

I

A

V

I

E

K

L

L

V

V

K

R

N

D

G

G

M

A

V

L

G

G

D

P

V

V

R

R

K

T

I

V

Q

V

S

T

S

D

F

H

G

G

F
|
F

A

T

K

I

L

-

P

-

F

G

D

D

E

P

G

K

H

Q

R
x
W

L
x
R

F

L

S

N

N

R

E

A

M

L

A

A

G

G

-

S

I

L

L

M

D
|
D

V

I

G

G

G

I

Y
|
Y

T

S

T

L

S

N

M

A

A

A

R

R

L

Y

I

L

A

T

G

G

I

-

G

-

T

-

S

-

G

-

V

-

M

E

E

E

P

P

A

V

E

A

V

V

T

N

A

A

L

V

G

E

H

S

L

T

G

D

R

R

T

S

G

D

-

P

-

R

-

F

-

G

-

E

V

V

D

E

E

D

W

I

T

I

S

N

A

F

L

Q

L

L

S

L

F

F

P

P

N

S

G

G

I

A

I

T

A

A

E

N

L

C

S

V

C

S

S

A

V

Y

S

S

L

V

E

N

Q

C

E

-

N

N

V

R

L

Y

R

R

I

V

L

S

G

G

T

P

K

K

G

G

R

W

I

V

E

E

V

I

D

D

Q

P

F

A

W

T

F

S

A
x
Y

G

Q

G

G

K

Q

P

-

G

-

T

-

S

-

I

A

I

M

R

R

I

A

V

Q

H

L

A

G

D

G

G

P

R

P

Q

A

E

P

E

R

V

E

P

P

L

A

V

P

E

Q

P

P

R

K

H

N

L

Q

Y

F

S

S

F

A

E

Q

V

L

E

D

A

H

A

L

G

S

D

E

A

C

I

I

R

L

A

T

G

G

R

R

T

E

E

-

F

-

A

-

Y

-

P

P

G

I

M

V

S

G

R

G

A

D

D

D

T

G

L

L

G

K

N

D

L

L

R

R

V

V

M

I

D

E

K

A

-

I

W

Y

R

R

A

A

active site: K100 (= K98), Y185 (= Y184)
binding beta-D-glucopyranose: K100 (= K98), F159 (= F157), R168 (≠ L168), D181 (= D180), Y185 (= Y184)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I76 (≠ A74), T77 (= T75), P78 (≠ V76), N79 (≠ H77), L81 (= L79), H82 (= H80), E99 (= E97), K100 (= K98), R128 (≠ M126), W167 (≠ R167), R168 (≠ L168), Y185 (= Y184), Y263 (≠ A265)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 10, 11, 12, 13, 35, 36, 37, 40, 58

5a03E Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with xylose (see paper)
30% identity, 42% coverage: 57:337/677 of query aligns to 59:327/335 of 5a03E

query
sites
5a03E

D

E

G

T

Y

F

D

D

A

R

L

I

I

I

D

D

D

N

P

P

G

D

I

V

D

D

A

I

V

V

Y

Y

I

V

A
x
I

T
|
T

V
x
P

H
x
N

P

S

L

L

H
|
H

A

R

E

P

W

F

A

T

I

E

K

R

A

A

E

R

K

A

G

G

K

K

H

H

V

V

L

M

C

C

E
|
E

K
|
K

P

P

M

M

G

A

L

N

S

T

T

V

A

A

E

D

A

C

D

E

A

A

M

M

F

I

E

A

A

A

A

C

R

K

K

K

A

A

G

G

T

R

F

K

M

L

A

M

E

I

A

G

Y

Y

M
x
R

Y

S

R

R

L

F

H

Q

P

A

L
x
H

T

N

A

I

R

E

I

A

V

I

E

K

L

L

V

V

K

R

N

D

G

G

M

A

V

L

G

G

D

P

V

V

R

R

K

T

I

V

Q

V

S

T

S

D

F

H

G

G

F
|
F

A

T

K

I

L

-

P

-

F

G

D

D

E

P

G

K

H

Q

R
x
W

L
x
R

F

L

S

N

N

R

E

A

M

L

A

A

G

G

-

S

I

L

L

M

D
|
D

V

I

G

G

G

I

Y
|
Y

T

S

T

L

S

N

M

A

A

A

R

R

L

Y

I

L

A

T

G

G

I

-

G

-

T

-

S

-

G

-

V

-

M

E

E

E

P

P

A

V

E

A

V

V

T

N

A

A

L

V

G
x
E

H

S

L
x
T

G

D

R
|
R

T

S

G

D

-

P

-

R

-

F

-

G

-

E

V

V

D

E

E

D

W

I

T

I

S

N

A

F

L

Q

L

L

S

L

F

F

P

P

N

S

G

G

I

A

I

T

A

A

E

N

L

C

S

V

C

S

S

A

V

Y

S

S

L

V

E

N

Q

C

E

-

N

N

V

R

L

Y

R

R

I

V

L

S

G

G

T

P

K

K

G

G

R

W

I

V

E

E

V

I

D

D

Q

P

F

A

W

T

F

S

A
x
Y

G

Q

G

G

K

Q

P

-

G

-

T

-

S

-

I

A

I
x
M

R

R

I

A

V

Q

H

L

A

G

D

G

G

P

R

P

Q

A

E

P

E
x
R

V
x
E

P

P

L

A

V

P

E

Q

P

P

R

K

H

N

L

Q

Y

F

S

S

F

A

E

Q

V

L

E

D

A

H

A

L

G

S

D

E

A

C

I

I

R

L

A

T

G

G

R

R

T

E

E

-

F

-

A

-

Y

-

P

P

G

I

M

V

S

G

R

G

A

D

D

D

T

G

L

L

G

K

N

D

L

L

R

R

V

V

M

I

D

E

K

A

-

I

W

Y

R

R

A

A

active site: K100 (= K98), Y185 (= Y184)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I76 (≠ A74), T77 (= T75), P78 (≠ V76), N79 (≠ H77), H82 (= H80), E99 (= E97), K100 (= K98), R128 (≠ M126), W167 (≠ R167), R168 (≠ L168), Y185 (= Y184), Y263 (≠ A265)
binding beta-D-xylopyranose: K100 (= K98), F159 (= F157), R168 (≠ L168), D181 (= D180), Y185 (= Y184), E205 (≠ G211), T207 (≠ L213), R209 (= R215)
binding alpha-D-xylopyranose: H134 (≠ L132), M268 (≠ I275), R279 (≠ E286), E280 (≠ V287)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 9, 10, 11, 12, 13, 35, 36, 37, 40, 58

5a02A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with glycerol (see paper)
30% identity, 42% coverage: 57:337/677 of query aligns to 59:327/335 of 5a02A

query
sites
5a02A

D

E

G

T

Y

F

D

D

A

R

L

I

I

I

D

D

D

N

P

P

G

D

I

V

D

D

A

I

V

V

Y

Y

I

V

A
x
I

T
|
T

V
x
P

H

N

P

S

L
|
L

H
|
H

A

R

E

P

W

F

A

T

I

E

K

R

A

A

E

R

K

A

G

G

K

K

H

H

V

V

L

M

C

C

E
|
E

K
|
K

P

P

M

M

G

A

L

N

S

T

T

V

A

A

E

D

A

C

D

E

A

A

M

M

F

I

E

A

A

A

A

C

R

K

K

K

A

A

G

G

T

R

F

K

M

L

A

M

E

I

A

G

Y

Y

M
x
R

Y

S

R

R

L

F

H

Q

P

A

L

H

T

N

A

I

R

E

I

A

V

I

E

K

L

L

V

V

K

R

N

D

G

G

M

A

V

L

G

G

D

P

V

V

R

R

K

T

I

V

Q

V

S

T

S

D

F

H

G

G

F

F

A

T

K

I

L

-

P

-

F

G

D

D

E

P

G

K

H

Q

R
x
W

L
x
R

F

L

S

N

N

R

E

A

M

L

A

A

G

G

-

S

I

L

L

M

D

D

V

I

G

G

G

I

Y
|
Y

T

S

T

L

S

N

M

A

A

A

R

R

L

Y

I

L

A

T

G

G

I

-

G

-

T

-

S

-

G

-

V

-

M

E

E

E

P

P

A

V

E

A

V

V

T

N

A

A

L

V

G

E

H

S

L

T

G

D

R

R

T

S

G

D

-

P

-

R

-

F

-

G

-

E

V

V

D

E

E

D

W

I

T

I

S

N

A

F

L

Q

L

L

S

L

F

F

P

P

N

S

G

G

I

A

I

T

A

A

E

N

L

C

S

V

C

S

S

A

V

Y

S

S

L

V

E

N

Q

C

E

-

N

N

V

R

L

Y

R

R

I

V

L

S

G

G

T

P

K

K

G

G

R

W

I

V

E

E

V

I

D

D

Q

P

F

A

W

T

F

S

A
x
Y

G

Q

G

G

K

Q

P

-

G

-

T

-

S

-

I

A

I

M

R

R

I

A

V

Q

H

L

A

G

D

G

G

P

R

P

Q

A

E

P

E

R

V

E

P

P

L

A

V

P

E

Q

P

P

R

K

H

N

L

Q

Y

F

S

S

F

A

E

Q

V

L

E

D

A

H

A

L

G

S

D

E

A

C

I

I

R

L

A

T

G

G

R

R

T

E

E

-

F

-

A

-

Y

-

P

P

G

I

M

V

S

G

R

G

A

D

D

D

T

G

L

L

G

K

N

D

L

L

R

R

V

V

M

I

D

E

K

A

-

I

W

Y

R

R

A

A

active site: K100 (= K98), Y185 (= Y184)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I76 (≠ A74), T77 (= T75), P78 (≠ V76), L81 (= L79), H82 (= H80), E99 (= E97), K100 (= K98), R128 (≠ M126), W167 (≠ R167), R168 (≠ L168), Y185 (= Y184), Y263 (≠ A265)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 10, 11, 12, 13, 35, 36, 37, 40, 58

B3TMR8 dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase; 3-ketoreductase; NADPH-dependent C3-ketoreductase; EC 1.1.1.384 from Actinomadura kijaniata (see paper)
27% identity, 49% coverage: 7:335/677 of query aligns to 10:323/332 of B3TMR8

query
sites
B3TMR8

I

I

R

R

W

V

G

G

I

V

L

I

G

G

P
x
C

G
x
A

N
x
D

I
|
I

A
|
A

K
x
W

D
x
R

F

R

F

A

A

L

G

P

A

A

L

L

Q

E

S

A

A

E

N

P

-

L

G

T

K

E

V

V

V

T

A

A

I

I

G

A

A
x
S

R
|
R

N

R

P

W

A

D

K

R

S

A

G

K

L

R

A

F

E

T

D

E

F

R

P

F

G

G

A

G

R

E

I

P

V

V

D

E

G

G

Y
|
Y

D

P

A

A

L

L

I

L

D

E

D

R

P

D

G

D

I

V

D

D

A

A

V

V

Y

Y

I

V

A

P

T
x
L

V

P

H

A

P

V

L

L

H
|
H

A

A

E

E

W

W

A

I

I

D

K

R

A

A

A

L

E

R

K

A

G

G

K

K

H

H

V

V

L

L

C

A

E

E

K
|
K

P

P

M

L

G

T

L

T

S

D

T

R

A

P

E

Q

A

A

D

E

A

R

M

L

F

F

E

A

A

V

A

A

R

R

K

E

A

R

G

G

T

L

F

L

M

L

A

M

E

E

A

N

Y

F

M

M

Y

F

R

L

L

H

H

H

P

P

L

Q

T

H

A

R

R

Q

I

V

V

A

E

D

L

M

V

L

K

D

N

E

G

G

M

V

V

I

G

G

D

E

V

I

R

R

K

S

I

F

Q

A

S

A

S

S

F

F

G

T

F

I

A

P

K

P

L

K

P

P

F

-

D

-

E

Q

G

G

H

D

R

I

L
x
R

F

Y

S

Q

N

A

E

D

M

V

A

G

G

G

G

A

I

L

L

L

D
|
D

V

I

G

G

G

V

Y
|
Y

T

P

T

I

S

R

M

A

A

A

R

G

L

L

I

F

A

L

G

G

I

-

G

-

T

-

S

-

G

-

V

-

M

-

E

-

P

-

A

A

E

D

V

L

T

E

A

F

L

V

G

G

H

A

L

V

G

L

R

R

T

H

G

E

V

R

D

D

E

R

W

D

T

V

-

V

-

G

S

N

A

A

L

L

S

T

F

T

P

R

N

Q

G

G

I

V

I

T

A

A

E

Q

L

L

S

T

C

F

S

G

V

M

S

E

L

H

E

A

Q

Y

E

T

N

N

V

N

L

Y

R

E

I

F

L

R

G

G

T

S

K

T

G

G

R

R

I

L

E

W

V

M

D

N

Q

R

F

V

W

F

F

-

A

-

G

-

K

T

P

P

G

P

G

A

T

T

S

-

I

Y

I

Q

R

P

I

V

V

V

H

H

A

I

D

E

G

R

R

Q

Q

D

E

H

E

A

V

E

P

Q

L

F

V

V

E

L

P

P

R

A

H

H

-

D

L

Q

Y

F

S

A

F

K

E

S

V

I

E

R

A

A

A

F

G

A

D

Q

A

A

I

V

R

L

A

S

G

G

R

E

T

-

E

-

F

-

A

-

Y

H

P

P

G

R

M

E

S

W

R

S

A

E

D

D

T

S

L

L

G

R

N

Q

L

A

R

S

V

L

M

V

D

D

K

A

W

V

R

R

17:23 (vs. 14:20, 29% identical) binding
SR 42:43 (≠ AR 38:39) binding
Y63 (= Y59) binding
L79 (≠ T75) binding
H84 (= H80) binding
K102 (= K98) active site, Proton donor; mutation K->A,M,Q: Loss of reductase activity.; mutation to E: Retains some activity, but the catalytic efficiency is strongly reduced.
R170 (≠ L168) binding
D182 (= D180) binding
Y186 (= Y184) mutation to F: Same affinity for dTDP-glucose and NADPH compared to the wild-type. Small reduction of the catalytic efficiency resulting from the conformational flexibility of the nicotinamide ring.

3rc1A Crystal structure of kijd10, a 3-ketoreductase from actinomadura kijaniata incomplex with NADP and tdp-benzene (see paper)
27% identity, 49% coverage: 7:335/677 of query aligns to 3:316/325 of 3rc1A

query
sites
3rc1A

I

I

R

R

W

V

G

G

I

V

L

I

G

G

P
x
C

G
x
A

N
x
D

I
|
I

A

A

K

W

D
x
R

F
x
R

F

A

A

L

G

P

A

A

L

L

Q

E

S

A

A

E

N

P

-

L

G

T

K

E

V

V

V

T

A

A

I

I

G

A

A
x
S

R
|
R

N

R

P

W

A

D

K

R

S

A

G

K

L

R

A

F

E

T

D

E

F

R

P

F

G

G

A

G

R

E

I

P

V

V

D

E

G

G

Y
|
Y

D

P

A

A

L

L

I

L

D

E

D

R

P

D

G

D

I

V

D

D

A

A

V

V

Y

Y

I

V

A
x
P

T
x
L

V
x
P

H

A

P

V

L

L

H
|
H

A

A

E

E

W

W

A

I

I

D

K

R

A

A

A

L

E

R

K

A

G

G

K

K

H

H

V

V

L

L

C

A

E
|
E

K
|
K

P

P

M

L

G

T

L

T

S

D

T

R

A

P

E

Q

A

A

D

E

A

R

M

L

F

F

E

A

A

V

A

A

R

R

K

E

A

R

G

G

T

L

F

L

M

L

A

M

E

E

A

N

Y

F

M

M

Y

F

R

L

L

H

H

H

P

P

L

Q

T

H

A

R

R

Q

I

V

V

A

E

D

L

M

V

L

K

D

N

E

G

G

M

V

V

I

G

G

D

E

V

I

R

R

K

S

I

F

Q

A

S

A

S

S

F
|
F

G

T

F
x
I

A
x
P

K

P

L

K

P

P

F

-

D

-

E

Q

G

G

H

D

R
x
I

L
x
R

F

Y

S

Q

N

A

E

D

M
x
V

A

G

G

G

G

A

I

L

L

L

D
|
D

V

I

G

G

G

V

Y
|
Y

T

P

T

I

S

R

M

A

A

A

R

G

L

L

I

F

A

L

G

G

I

-

G

-

T

-

S

-

G

-

V

-

M

-

E

-

P

-

A

A

E

D

V

L

T

E

A

F

L

V

G

G

H

A

L

V

G

L

R

R

T

H

G

E

V

R

D

D

E

R

W

D

T

V

-

V

-

G

S

N

A

A

L

L

S

T

F

T

P

R

N

Q

G

G

I

V

I

T

A

A

E

Q

L

L

S

T

C

F

S

G

V

M

S

E

L

H

E

A

Q
x
Y

E

T

N

N

V

N

L

Y

R

E

I

F

L

R

G

G

T

S

K

T

G

G

R

R

I

L

E

W

V

M

D

N

Q

R

F

V

W

F

F

-

A

-

G

-

K
x
T

P

P

G

P

G

A

T

T

S

-

I

Y

I

Q

R

P

I

V

V

V

H

H

A

I

D

E

G

R

R

Q

Q

D

E

H

E

A

V

E

P

Q

L

F

V

V

E

L

P

P

R

A

H
|
H

-

D

L

Q

Y

F

S

A

F
x
K

E

S

V

I

E

R

A

A

A

F

G

A

D

Q

A

A

I

V

R

L

A

S

G

G

R

E

T

-

E

-

F

-

A

-

Y

H

P

P

G

R

M

E

S

W

R

S

A

E

D

D

T

S

L

L

G

R

N

Q

L

A

R

S

V

L

M

V

D

D

K

A

W

V

R

R

active site: K95 (= K98), Y179 (= Y184)
binding nadp nicotinamide-adenine-dinucleotide phosphate: C10 (≠ P14), A11 (≠ G15), D12 (≠ N16), I13 (= I17), S35 (≠ A38), R36 (= R39), Y56 (= Y59), P71 (≠ A74), L72 (≠ T75), P73 (≠ V76), H77 (= H80), E94 (= E97), K95 (= K98), I162 (≠ R167), R163 (≠ L168), V168 (≠ M173), D175 (= D180), Y179 (= Y184)
binding phosphate ion: H278 (= H294), K283 (≠ F298)
binding 5'-O-[(S)-hydroxy{[(S)-hydroxy(phenoxy)phosphoryl]oxy}phosphoryl]thymidine: R16 (≠ D20), R17 (≠ F21), F152 (= F155), I154 (≠ F157), P155 (≠ A158), Y233 (≠ Q244), T253 (≠ K268)

Q2I8V6 1,5-anhydro-D-fructose reductase; Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming); EC 1.1.1.292 from Ensifer adhaerens (Sinorhizobium morelense) (see paper)
29% identity, 44% coverage: 8:307/677 of query aligns to 3:294/333 of Q2I8V6

query
sites
Q2I8V6

R

R

W

W

G

G

I

L

L

I

G

G

P
x
A

G
x
S

N
x
T

I
|
I

A
|
A

K

R

D

E

F

W

F

V

A

I

G

G

A

A

L

I

Q

R

S

A

A

T

N

G

G

G

K

E

V

V

A

S

I

M

G

M

A
x
S

R
x
T

N

S

P

A

A

E

K
x
R

-

G

S

A

G

A

L

Y

A

A

E

T

D

E

F

N

P

G

G

I

A

G

R

K

I

S

V

V

D

T

G

S

Y

V

D

E

A

E

L

L

I

V

D

G

D

D

P

P

G

D

I

V

D

D

A

A

V

V

Y

Y

I

V

A

S

T
|
T

V
x
T

H
x
N

P
x
E

L
|
L

H
|
H

A

R

E

E

W

Q

A

T

I

L

K

A

A

A

A

I

E

R

K

A

G

G

K

K

H

H

V

V

L

L

C

C

E
|
E

K
|
K

P

P

M

L

G

A

L

M

S

T

T

L

A

E

E

D

A

A

D

R

A

E

M

M

F

V

E

V

A

A

R

R

K

E

A

A

G

G

T

V

F

V

M

L

A

G

E

T

A
x
N

Y

H

M

H

Y

L

R

R

L

N

H

A

P

A

L

A

T

H

A

R

R

A

I

M

V

R

E

D

L

A

V

I

K

A

N

E

G

G

M

R

V

I

G

G

D

-

V

-

R

R

K

P

I

I

Q

A

S

A

S

R

F

V

G

F

F

H

A

A

K

V

L

Y

-

L

-

P

P

P

F

H

D

L

E

Q

G

G

H
x
W

R
|
R

L

L

F

E

S

R

N

P

E

E

M

A

A

G

G

G

G

V

I

I

L

L

D
|
D

V

I

G

T

G

V

Y
x
H

T

D

T

A

S

D

M

T

A

L

R

R

L

F

I

V

A

L

G

N

I

-

G

-

T

-

S

-

G

-

V

-

M

D

E

D

P

P

A

A

E

E

V

A

T

V

A

A

L

I

G

S

H

H

-

S

-

A

-

G

L

M

G
|
G

R

K

T

E

G

G

V

V

D

E

E

D

W

G

T

V

S

M

A

G

L

G

L

V

S

R

F

F

P

Q

N

S

G

G

I

V

I

I

A

A

E

Q

L

F

S

H

C

D

S

A

V

F

S

T

L

T

E

K

-

F

Q

A

E

E

N

T

V

G

L

F

R

E

I

V

L

H

G

G

T

T

K

E

G

G

R

S

I

L

E

I

V

G

D

R

Q

N

F

V

W

M

F

T

A

Q

G

-

K

K

P

P

G

V

G

G

T

T

S

V

I

T

I

L

R

R

I

-

V

-

H

N

A

A

D

E

G

G

R

-

Q

E

E

S

E

Q

V

L

P

P

L

L

V

-

E

D

P

P

R

A

H

N

L

L

Y
|
Y

S

E

F

T

E

A

V

L

E

A

A

A

A

F

G

H

D

S

A

A

I

I

ASTI 9:12 (≠ PGNI 14:17) binding
S10 (≠ G15) mutation to G: Almost no effect.
A13 (= A18) mutation to G: Can use NAD as cosubstrate as well as NADP.
S33 (≠ A38) mutation to D: No activity.
ST 33:34 (≠ AR 38:39) binding
R38 (≠ K43) binding
TTNELH 71:76 (≠ TVHPLH 75:80) binding
EK 93:94 (= EK 97:98) binding
K94 (= K98) mutation to G: Less than 1% remaining activity.
N120 (≠ A124) binding
WR 162:163 (≠ HR 166:167) binding
D176 (= D180) mutation to A: Less than 1% remaining activity.
H180 (≠ Y184) mutation to A: Less than 2% remaining activity.
G206 (= G214) mutation to I: No effect.
Y283 (= Y296) binding

4koaA Crystal structure analysis of 1,5-anhydro-d-fructose reductase from sinorhizobium meliloti (see paper)
30% identity, 44% coverage: 7:307/677 of query aligns to 2:294/333 of 4koaA

query
sites
4koaA

I

I

R

R

W

W

G

G

I

L

L

I

G
|
G

P
x
A

G
x
S

N
x
T

I
|
I

A

A

K

R

D

E

F

W

F

V

A

I

G

G

A

A

L

I

Q

R

S

A

A

A

N

G

G

G

K

E

V

V

A

S

I

V

G

M

A
x
S

R
x
S

N

S

P

A

A

E

K
x
R

-

G

S

E

G

A

L

Y

A

A

E

A

D

E

F

N

P

G

G

I

A

A

R

K

I

A

V

V

D

T

G

S

Y

V

D

D

A

D

L

L

I

V

D

G

D

D

P

P

G

D

I

V

D

D

A

A

V

V

Y

Y

I

I

A

S

T
|
T

V

T

H
x
N

P

E

L

L

H
|
H

A

H

E

G

W

Q

A

A

I

L

K

A

A

A

A

I

E

R

K

A

G

G

K

K

H

H

V

V

L

L

C

C

E

E

K
|
K

P

P

M

L

G

A

L

M

S

N

T

L

A

N

E

D

A

G

D

C

A

E

M

M

F

V

E

L

A

K

A

A

R

C

K

E

A

A

G

G

T

V

F

V

M

L

A

G

E

T

A

N

Y

H

M

H

Y

L

R

R

L

N

H

A

P

A

L

T

T

H

A

R

R

A

I

M

V

R

E

E

L

A

V

I

K

A

N

A

G

G

M

R

V

I

G

G

D

-

V

-

R

R

K

P

I

I

Q

A

S

A

S

R

F

V

G

F

F

H

A

A

K

V

L

Y

-

L

-

P

P

P

F

H

D

L

E
x
Q

G

G

H

W

R

R

L

L

F

D

S

K

N

P

E

E

M

A

A

G

G

G

G

V

I

I

L

L

D

D

V

I

G

T

G

V

Y
x
H

T

D

T

A

S

D

M

T

A

L

R

R

L

F

I

V

A

L

G

N

I

-

G

-

T

-

S

-

G

-

V

-

M

D

E

D

P

P

A

I

E

E

V

A

T

V

A

A

L

I

G

S

H

H

-

S

-

A

-

G

L

M

G

G

R

K

T

E

G

G

V

L

D

E

E

D

W

G

T

V

S

M

A

G

L

V

L

L

S

R

F

F

P

R

N

S

G

G

I

V

I

I

A

A

E

Q

L

F

S

H

C

D

S

A

V

F

S

T

L

T

E

K

-

F

Q

A

E

E

N

T

V

G

L

L

R

E

I

V

L

H

G

G

T

T

K

A

G

G

R

S

I

L

E

I

V

G

D

R

Q

N

F

V

W

M

F

T

A

Q

G

-

K

R

P

P

G

V

G

G

T

T

S

V

I

V

I

L

R

R

I

-

V

-

H

N

A

E

D

E

G

G

R

-

Q

E

E

S

E

E

V

L

P

P

L

L

V

-

E

D

P

H

R

R

H

N

L

L

Y

Y

S

E

F

T

E

A

V

I

E

A

A

A

A

F

G

H

D

S

A

A

I

I

active site: K94 (= K98), H180 (≠ Y184)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G13), A9 (≠ P14), S10 (≠ G15), T11 (≠ N16), I12 (= I17), S33 (≠ A38), S34 (≠ R39), R38 (≠ K43), T71 (= T75), N73 (≠ H77), H76 (= H80), K94 (= K98), Q160 (≠ E164)

1h6dA Oxidized precursor form of glucose-fructose oxidoreductase from zymomonas mobilis complexed with glycerol (see paper)
30% identity, 31% coverage: 1:212/677 of query aligns to 28:240/383 of 1h6dA

query
sites
1h6dA

M

M

N

P

E

E

P

D

T

R

R

R

I

F

R

G

W

Y

G

A

I

I

L

V

G
|
G

P
x
L

G
|
G

N
x
K

I
x
Y

A

A

-

L

K

N

D

Q

F

I

F

L

A

P

G

G

A

F

L

A

Q

G

S

C

A

Q

N

H

G

S

K

R

V

I

V

E

A

A

I

L

G

V

A
x
S

R
x
G

N

N

P

A

A

E

K
|
K

S

A

G

K

L

I

A

V

E

A

D

A

F

E

P

Y

G

G

A

V

-

D

-

P

-

R

R

K

I

I

V

Y

D

D

-
x
Y

-

S

G

N

Y

F

D

D

A

K

L

I

I

A

D

K

D

D

P

P

G

K

I

I

D

D

A

A

V

V

Y

Y

I

I

A
x
I

T
x
L

V
x
P

H
x
N

P

S

L

L

H
|
H

A

A

E

E

W

F

A

A

I

I

K

R

A

A

A

F

E

K

K

A

G

G

K

K

H

H

V

V

L

M

C

C

E
|
E

K
|
K

P

P

M

M

G

A

L

T

S

S

T

V

A

A

E

D

A

C

D

Q

A

R

M

M

F

I

E

D

A

A

R

K

K

A

A

A

G

N

T

K

F

K

M

L

A

M

E

I

A

G

Y

Y

M
x
R

Y

C

R

H

L

Y

H

D

P

P

L

M

T

N

A

R

R

A

I

A

V

V

E

K

L

L

V

I

K

R

N

E

G

N

M

Q

V

L

G

G

D

K

V

L

R

G

K

M

I

V

Q

T

S

T

S

D

F

N

G

S

F

D

A

V

K

M

L

D

P

Q

F

N

D

D

E

P

G
x
A

H

Q

R

Q

-
x
W

L
x
R

F

L

S

R

N

R

E

E

M

L

A

A

G

G

-

S

I

L

L

M

D
|
D

V

I

G

G

G

I

Y
|
Y

T

G

T

L

S

N

M

G

A

T

R

R

L

Y

I

L

A

L

G

G

I

-

G

-

T

-

S

-

G

-

V

-

M

E

E

E

P

P

A

I

E

E

V

V

T

R

A

A

L

Y

G

T

H

Y

active site: K131 (= K98), Y219 (= Y184)
binding glycerol: K131 (= K98), R202 (≠ L168), D215 (= D180), Y219 (= Y184)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G40 (= G13), L41 (≠ P14), G42 (= G15), K43 (≠ N16), Y44 (≠ I17), S66 (≠ A38), G67 (≠ R39), K71 (= K43), Y89 (vs. gap), I107 (≠ A74), L108 (≠ T75), P109 (≠ V76), N110 (≠ H77), H113 (= H80), E130 (= E97), K131 (= K98), R159 (≠ M126), A198 (≠ G165), W201 (vs. gap), R202 (≠ L168), Y219 (= Y184)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 17, 18, 19, 298

1rydA Crystal structure of glucose-fructose oxidoreductase from zymomonas mobilis
30% identity, 31% coverage: 1:212/677 of query aligns to 26:238/381 of 1rydA

query
sites
1rydA

M

M

N

P

E

E

P

D

T

R

R

R

I

F

R

G

W

Y

G

A

I

I

L

V

G

G

P
x
L

G
|
G

N
x
K

I
x
Y

A

A

-

L

K

N

D

Q

F

I

F

L

A

P

G

G

A

F

L

A

Q

G

S

C

A

Q

N

H

G

S

K

R

V

I

V

E

A

A

I

L

G

V

A
x
S

R
x
G

N

N

P

A

A

E

K
|
K

S

A

G

K

L

I

A

V

E

A

D

A

F

E

P

Y

G

G

A

V

-

D

-

P

-

R

R

K

I

I

V

Y

D

D

-
x
Y

-

S

G

N

Y

F

D

D

A

K

L

I

I

A

D

K

D

D

P

P

G

K

I

I

D

D

A

A

V

V

Y

Y

I

I

A

I

T
x
L

V
x
P

H
x
N

P

S

L
|
L

H
|
H

A

A

E

E

W

F

A

A

I

I

K

R

A

A

A

F

E

K

K

A

G

G

K

K

H

H

V

V

L

M

C

C

E
|
E

K
|
K

P

P

M

M

G

A

L

T

S

S

T

V

A

A

E

D

A

C

D

Q

A

R

M

M

F

I

E

D

A

A

R

K

K

A

A

A

G

N

T

K

F

K

M

L

A

M

E

I

A

G

Y

Y

M
x
R

Y

C

R

H

L

Y

H

D

P

P

L

M

T

N

A

R

R

A

I

A

V

V

E

K

L

L

V

I

K

R

N

E

G

N

M

Q

V

L

G

G

D

K

V

L

R

G

K

M

I

V

Q

T

S

T

S

D

F

N

G

S

F

D

A

V

K

M

L

D

P

Q

F

N

D

D

E

P

G
x
A

H

Q

R

Q

-
x
W

L
x
R

F

L

S

R

N

R

E

E

M

L

A

A

G

G

-

S

I

L

L

M

D

D

V

I

G

G

G

I

Y
|
Y

T

G

T

L

S

N

M

G

A

T

R

R

L

Y

I

L

A

L

G

G

I

-

G

-

T

-

S

-

G

-

V

-

M

E

E

E

P

P

A

I

E

E

V

V

T

R

A

A

L
x
Y

G

T

H

Y

active site: K129 (= K98), Y217 (= Y184)
binding alpha-D-glucopyranose: Y236 (≠ L210)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L39 (≠ P14), G40 (= G15), K41 (≠ N16), Y42 (≠ I17), S64 (≠ A38), G65 (≠ R39), K69 (= K43), Y87 (vs. gap), L106 (≠ T75), P107 (≠ V76), N108 (≠ H77), L110 (= L79), H111 (= H80), E128 (= E97), K129 (= K98), R157 (≠ M126), A196 (≠ G165), W199 (vs. gap), R200 (≠ L168), Y217 (= Y184)

Sites not aligning to the query:

1evjA Crystal structure of glucose-fructose oxidoreductase (gfor) delta1-22 s64d (see paper)
33% identity, 23% coverage: 58:212/677 of query aligns to 60:209/340 of 1evjA

query
sites
1evjA

G

N

Y

F

D

D

A

K

L

I

I

A

D

K

D

D

P

P

G

K

I

I

D

D

A

A

V

V

Y

Y

I

I

A

I

T
x
L

V
x
P

H
x
N

P

S

L

L

H
|
H

A

A

E

E

W

F

A

A

I

I

K

R

A

A

A

F

E

K

K

A

G

G

K

K

H

H

V

V

L

M

C

C

E
|
E

K
|
K

P

P

M

M

G

A

L

T

S

S

T

V

A

A

E

D

A

C

D

Q

A

R

M

M

F

I

E

D

A

A

R

K

K

A

A

A

G

N

T

K

F

K

M

L

A

M

E

I

A

G

Y

Y

M
x
R

Y

C

R

H

L

Y

H

D

P

P

L

M

T

N

A

R

R

A

I

A

V

V

E

K

L

L

V

I

K

R

N

E

G

N

M

Q

V

L

G

G

D

K

V

L

R

G

K

M

I

V

Q

T

S

T

S

D

F

N

G

S

F

D

A

V

K

M

L

D

P

Q

F

N

D

D

E

P

G

A

H

Q

R

Q

-
x
W

L
x
R

F

L

S

R

N

R

E

E

M

L

A

A

G

G

-

S

I

L

L

M

D

D

V

I

G

G

G

I

Y
|
Y

T

G

T

L

S

N

M

G

A

T

R

R

L

Y

I

L

A

L

G

G

I

-

G

-

T

-

S

-

G

-

V

-

M

E

E

E

P

P

A

I

E

E

V

V

T

R

A

A

L

Y

G

T

H

Y

active site: K100 (= K98), Y188 (= Y184)
binding nicotinamide-adenine-dinucleotide: L77 (≠ T75), P78 (≠ V76), N79 (≠ H77), H82 (= H80), E99 (= E97), K100 (= K98), R128 (≠ M126), W170 (vs. gap), R171 (≠ L168), Y188 (= Y184)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 9, 10, 11, 12, 13, 35, 267

1pz0A Structure of NADPH-dependent family 11 aldo-keto reductase akr11a(holo) (see paper)
25% identity, 39% coverage: 405:666/677 of query aligns to 47:304/311 of 1pz0A

query
sites
1pz0A

G

G

G

V

N

T

L

M

V

L

D
|
D

T

T

A

A

F

Y

L

I

Y
|
Y

G

G

N

I

G

G

V

R

Q

S

D

E

R

E

L

L

V

I

G

G

E

E

W

V

M

L

R

R

S

E

R

F

G

N

V

-

R

R

Q

E

E

D

T

V

V

V

V

I

I

A

A

T

K

K

G

A

A

A

H

H

-

R

-

K

-

Q

-

G

S
x
N

P

D

L

F

C

V

Y

F

-

D

-

N

-

S

P

P

D

D

V

F

I

L

G

K

K

K

Q

S

L

V

T

D

T

E

S

S

L

L

E

K

R

R

M

L

G

N

T

T

D

D

Y

Y

V

I

D

D

I

L

Y

F

F

Y

M

I

H
|
H

R

F

D

P

N

D

P

E

D

H

I

T

P

P

V

K

G

D

E

E

F

A

V

V

D

N

A

A

M

L

D

N

A

E

E

M

V

K

A

K

A

A

G

G

R

K

I

I

R

R

G

S

P

-

I

I

G

G

G

V

S

S

N

N

W

F

T

S

R

L

E

E

R

Q

F

L

E

K

E

E

A

A

I

-

A

-

Y

-

A

-

E

-

R

-

A

-

G

-

K

-

T

-

K

-

P

-

S

-

V

-

L

-

S

-

N

N

N

K

F

D

S

G

L

L

A

V

E

D

M

V

V

L

Q
|
Q

P

G

V

E

W

Y

A

N

G

L

C

L

I

N

S

R

S

E

S

A

D

E

D

K

A

T

W

F

M

F

R

P

W

Y

L

T

E

K

E

E

N

H

D

N

V

I

T

S

N

F

F

I

A

P

W
x
Y

S
x
F

S
x
P

Q
x
L

A

V

R
x
S

G
|
G

F

L

T

-

D

-

R

-

A
|
A

G

G

R
x
K

G

Y

K

T

L

E

D

D

-

T

-

F

-

P

-

E

-

G

D

D

L

L

E

R

L

N

A

E

R

Q

S

E

W

H

Y

F

S

K

E

G

G

E

N

R

F

F

A

K

R

E

R

N

D

I

R

R

A

K

I

V

-

N

-

K

-

L

-

A

A

P

L

I

G

A

R

E

K

K

L

H

G

N

K

V

D

D

P

I

I

P

Q

H

I

I

A

V

L

L

A

A

Y

W

V

Y

L

L

A

A

Q

R

K

P

G

E

R

I

V

D

I

I

P

L

L

I

I

P

G

G

P

A

R

K

L

R

L

A

A

D

E

Q

L

L

N

I

H

D

S

N

L

I

D

K

A

T

F

A

A

D

V

V

T

T

L

L

S

S

P

Q

G

E

D

D

V

I

Q

S

W

F

L

I

active site: D52 (= D410), Y57 (= Y415), N91 (≠ S446), H124 (= H476)
binding nadp nicotinamide-adenine-dinucleotide phosphate: H124 (= H476), Q174 (= Q543), Y202 (≠ W571), F203 (≠ S572), P204 (≠ S573), L205 (≠ Q574), S207 (≠ R576), G208 (= G577), A211 (= A583), K213 (≠ R585)

P46336 Aldo-keto reductase IolS; AKR11A; Vegetative protein 147; VEG147; EC 1.1.1.- from Bacillus subtilis (strain 168) (see paper)
25% identity, 39% coverage: 405:666/677 of query aligns to 48:305/310 of P46336

query
sites
P46336

G

G

G

V

N

T

L

M

V

L

D

D

T

T

A

A

F

Y

L

I

Y

Y

G

G

N

I

G

G

V

R

Q

S

D

E

R

E

L

L

V

I

G

G

E

E

W

V

M

L

R

R

S

E

R

F

G

N

V

-

R

R

Q

E

E

D

T

V

V

V

V

I

I

A

A

T

K

K

G

A

A

A

H

H

-

R

-

K

-

Q

-

G

S

N

P

D

L

F

C

V

Y

F

-

D

-

N

-

S

P

P

D

D

V

F

I

L

G

K

K

K

Q

S

L

V

T

D

T

E

S

S

L

L

E

K

R

R

M

L

G

N

T

T

D

D

Y

Y

V

I

D

D

I

L

Y

F

F

Y

M

I

H

H

R

F

D

P

N

D

P

E

D

H

I

T

P

P

V

K

G

D

E

E

F

A

V

V

D

N

A

A

M

L

D

N

A

E

E

M

V

K

A

K

A

A

G

G

R

K

I

I

R

R

G

S

P

-

I

I

G

G

G

V

S
|
S

N
|
N

W

F

T

S

R

L

E

E

R

Q

F

L

E

K

E

E

A

A

I

-

A

-

Y

-

A

-

E

-

R

-

A

-

G

-

K

-

T

-

K

-

P

-

S

-

V

-

L

-

S

-

N

N

N

K

F

D

S

G

L

L

A

V

E

D

M

V

V

L

Q
|
Q

P

G

V

E

W

Y

A

N

G

L

C

L

I

N

S

R

S

E

S

A

D

E

D

K

A

T

W

F

M

F

R

P

W

Y

L

T

E

K

E

E

N

H

D

N

V

I

T

S

N

F

F

I

A

P

W
x
Y

S
x
F

S
x
P

Q
x
L

A
x
V

R
x
S

G

G

F

L

T

-

D

-

R

-

A

A

G

G

R
x
K

G

Y

K

T

L

E

D

D

-

T

-

F

-

P

-

E

-

G

D

D

L

L

E

R

L

N

A

E

R

Q

S

E

W

H

Y

F

S

K

E

G

G

E

N

R

F

F

A

K

R

E

R

N

D

I

R

R

A

K

I

V

-

N

-

K

-

L

-

A

A

P

L

I

G

A

R

E

K

K

L

H

G

N

K

V

D

D

P

I

I

P

Q

H

I

I

A

V

L

L

A

A

Y

W

V

Y

L

L

A

A

Q

R

K

P

G

E

R

I

V

D

I

I

P

L

L

I

I

P

G

G

P

A

R

K

L

R

L

A

A

D

E

Q

L

L

N

I

H

D

S

N

L

I

D

K

A

T

F

A

A

D

V

V

T

T

L

L

S

S

P

Q

G

E

D

D

V

I

Q

S

W

F

L

I

SN 155:156 (= SN 507:508) binding
Q175 (= Q543) binding
YFPLVS 203:208 (≠ WSSQAR 571:576) binding
K214 (≠ R585) binding

Query Sequence

>SMa2313 FitnessBrowser__Smeli:SMa2313
MNEPTRIRWGILGPGNIAKDFFAGALQSANGKVVAIGARNPAKSGLAEDFPGARIVDGYD
ALIDDPGIDAVYIATVHPLHAEWAIKAAEKGKHVLCEKPMGLSTAEADAMFEAARKAGTF
MAEAYMYRLHPLTARIVELVKNGMVGDVRKIQSSFGFAKLPFDEGHRLFSNEMAGGGILD
VGGYTTSMARLIAGIGTSSGVMEPAEVTALGHLGRTGVDEWTSALLSFPNGIIAELSCSV
SLEQENVLRILGTKGRIEVDQFWFAGGKPGGTSIIRIVHADGRQEEVPLVEPRHLYSFEV
EAAGDAIRAGRTEFAYPGMSRADTLGNLRVMDKWRAAIGLEYEGEKHTTRTRTVRGDKLA
RKTSLVRSGRIDGLQKEISHAALGLMEFSTFSSAAIVLDAFFEAGGNLVDTAFLYGNGVQ
DRLVGEWMRSRGVRQETVVIAKGAHSPLCYPDVIGKQLTTSLERMGTDYVDIYFMHRDNP
DIPVGEFVDAMDAEVAAGRIRGPIGGSNWTRERFEEAIAYAERAGKTKPSVLSNNFSLAE
MVQPVWAGCISSSDDAWMRWLEENDVTNFAWSSQARGFFTDRAGRGKLDDLELARSWYSE
GNFARRDRAIALGRKLGKDPIQIALAYVLAQKGRVIPLIGPRLLAELNHSLDAFAVTLSP
GDVQWLRDGDPGESSAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory