SitesBLAST
Comparing SMc00771 SMc00771 putrescine ABC transporter ATP-binding protein to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
45% identity, 96% coverage: 18:381/381 of query aligns to 11:374/378 of P69874
- C26 (≠ K32) mutation to A: Lower ATPase activity and transport efficiency.
- F27 (= F33) mutation to L: Lower ATPase activity and transport efficiency.
- F45 (= F51) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (= C60) mutation to T: Loss of ATPase activity and transport.
- L60 (= L66) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (= L82) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (= V141) mutation to M: Loss of ATPase activity and transport.
- D172 (= D178) mutation to N: Loss of ATPase activity and transport.
- C276 (vs. gap) mutation to A: Lower ATPase activity and transport efficiency.
- E297 (= E303) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.
2d62A Crystal structure of multiple sugar binding transport atp- binding protein
44% identity, 83% coverage: 24:339/381 of query aligns to 7:332/375 of 2d62A
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
43% identity, 83% coverage: 24:340/381 of query aligns to 4:318/369 of P19566
- L86 (= L106) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P180) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D185) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- E306 (≠ G328) mutation to K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
42% identity, 83% coverage: 24:340/381 of query aligns to 3:319/374 of 2awnB
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
42% identity, 83% coverage: 24:340/381 of query aligns to 3:319/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F33), S37 (= S58), G38 (= G59), C39 (= C60), G40 (= G61), K41 (= K62), S42 (= S63), T43 (= T64), Q81 (= Q102), R128 (= R149), A132 (≠ Q153), S134 (= S155), G136 (= G157), Q137 (= Q158), E158 (= E179), H191 (= H212)
- binding magnesium ion: S42 (= S63), Q81 (= Q102)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
42% identity, 83% coverage: 24:340/381 of query aligns to 3:319/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ F33), G38 (= G59), C39 (= C60), G40 (= G61), K41 (= K62), S42 (= S63), T43 (= T64), R128 (= R149), S134 (= S155), Q137 (= Q158)
- binding beryllium trifluoride ion: S37 (= S58), G38 (= G59), K41 (= K62), Q81 (= Q102), S134 (= S155), G136 (= G157), H191 (= H212)
- binding magnesium ion: S42 (= S63), Q81 (= Q102)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
42% identity, 83% coverage: 24:340/381 of query aligns to 3:319/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ F33), V17 (≠ A38), G38 (= G59), C39 (= C60), G40 (= G61), K41 (= K62), S42 (= S63), T43 (= T64), R128 (= R149), A132 (≠ Q153), S134 (= S155), Q137 (= Q158)
- binding tetrafluoroaluminate ion: S37 (= S58), G38 (= G59), K41 (= K62), Q81 (= Q102), S134 (= S155), G135 (= G156), G136 (= G157), E158 (= E179), H191 (= H212)
- binding magnesium ion: S42 (= S63), Q81 (= Q102)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
42% identity, 83% coverage: 24:340/381 of query aligns to 3:319/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ F33), V17 (≠ A38), G38 (= G59), C39 (= C60), G40 (= G61), K41 (= K62), S42 (= S63), T43 (= T64), R128 (= R149), A132 (≠ Q153), S134 (= S155), Q137 (= Q158)
- binding magnesium ion: S42 (= S63), Q81 (= Q102)
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
42% identity, 83% coverage: 24:340/381 of query aligns to 4:320/371 of P68187
- A85 (= A105) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ P126) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (= V134) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ M137) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ K139) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ E144) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G157) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D178) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (= R248) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- F241 (≠ I259) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- W267 (≠ A285) mutation to G: Normal maltose transport but constitutive mal gene expression.
- G278 (= G293) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- S282 (≠ A297) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G284 (≠ A299) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G302 (= G322) mutation to D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- E308 (≠ G328) mutation to Q: Maltose transport is affected but retains ability to interact with MalT.
Sites not aligning to the query:
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
42% identity, 83% coverage: 24:340/381 of query aligns to 1:317/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ F33), S35 (= S58), G36 (= G59), C37 (= C60), G38 (= G61), K39 (= K62), S40 (= S63), T41 (= T64), R126 (= R149), A130 (≠ Q153), S132 (= S155), G134 (= G157), Q135 (= Q158)
1g291 Malk (see paper)
49% identity, 65% coverage: 24:272/381 of query aligns to 4:258/372 of 1g291
- binding magnesium ion: D69 (≠ G89), E71 (vs. gap), K72 (vs. gap), K79 (≠ Y93), D80 (≠ R94)
- binding pyrophosphate 2-: S38 (= S58), G39 (= G59), C40 (= C60), G41 (= G61), K42 (= K62), T43 (≠ S63), T44 (= T64)
Sites not aligning to the query:
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
44% identity, 84% coverage: 24:342/381 of query aligns to 7:313/353 of 1vciA
P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
46% identity, 74% coverage: 24:306/381 of query aligns to 4:288/393 of P9WQI3
- H193 (= H212) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.
8hprD Lpqy-sugabc in state 4 (see paper)
49% identity, 62% coverage: 24:260/381 of query aligns to 3:242/362 of 8hprD
- binding adenosine-5'-triphosphate: Y12 (≠ F33), S38 (= S58), C40 (= C60), G41 (= G61), K42 (= K62), S43 (= S63), T44 (= T64), Q82 (= Q102), R129 (= R149), Q133 (= Q153), S135 (= S155), G136 (= G156), G137 (= G157), Q159 (≠ E179), H192 (= H212)
- binding magnesium ion: S43 (= S63), Q82 (= Q102)
8hprC Lpqy-sugabc in state 4 (see paper)
49% identity, 62% coverage: 24:260/381 of query aligns to 3:242/363 of 8hprC
- binding adenosine-5'-triphosphate: Y12 (≠ F33), S38 (= S58), G39 (= G59), G41 (= G61), K42 (= K62), S43 (= S63), Q82 (= Q102), Q133 (= Q153), G136 (= G156), G137 (= G157), Q138 (= Q158), H192 (= H212)
- binding magnesium ion: S43 (= S63), Q82 (= Q102)
8hplC Lpqy-sugabc in state 1 (see paper)
49% identity, 62% coverage: 24:260/381 of query aligns to 3:240/384 of 8hplC
3d31A Modbc from methanosarcina acetivorans (see paper)
39% identity, 81% coverage: 23:330/381 of query aligns to 1:304/348 of 3d31A
Sites not aligning to the query:
1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
37% identity, 81% coverage: 24:333/381 of query aligns to 4:310/353 of 1oxvD
1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
37% identity, 81% coverage: 24:333/381 of query aligns to 4:310/353 of 1oxvA
1oxuA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
37% identity, 81% coverage: 24:333/381 of query aligns to 4:310/353 of 1oxuA
Query Sequence
>SMc00771 SMc00771 putrescine ABC transporter ATP-binding protein
MMKSLGSIRRSFAPWADPASKPFISVKNVTKKFGDFTAVDDLSLNIYTREFFALLGASGC
GKSTLLRMLAGFEQPTSGEIILDGQSLAGIPPYRRPVNMMFQSYALFPHMTVENNVAFGL
KQDGMPKADIAERVAQMLKLVKLEKFAKRKPHQLSGGQRQRVALARSLAKRPKVLLLDEP
LGALDKKLREETQFELMDLQQELGLTFVVVTHDQEEAMTMADRIAVMSHGKVVQVATPAE
IYEAPNSRFVADFIGDVNIFDGKVTSAEDGYIRVETTGGIPVRMASPEKPGNGAKAAVAI
RPEKIRIGRQPPAHAPVNAAEGEIWDIGYLGDMTVFHIRLKDGKVVKASSLNAVRAVEDP
LGYDQQVWISFGDDAGVVLKD
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory