SitesBLAST
Comparing SMc01455 FitnessBrowser__Smeli:SMc01455 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
48% identity, 93% coverage: 33:467/468 of query aligns to 20:456/456 of 8jdsA
- binding flavin-adenine dinucleotide: E32 (≠ T45), P68 (= P81), G70 (= G83), T71 (= T84), G72 (= G85), T73 (≠ S86), G74 (≠ S87), G78 (≠ H91), V79 (= V92), L90 (≠ M103), P132 (= P145), G133 (= G146), A134 (= A147), G140 (= G153), M141 (= M154), A143 (≠ S156), T144 (= T157), A146 (= A159), S147 (= S160), E200 (= E210), G201 (= G211), I206 (≠ V216), W323 (≠ Y335), E414 (= E426), H415 (= H427), N451 (= N462)
- binding manganese (ii) ion: H370 (= H382), H377 (= H389), E414 (= E426)
- binding pyruvic acid: R319 (= R331), H370 (= H382), H377 (= H389), H415 (= H427)
8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
48% identity, 93% coverage: 33:467/468 of query aligns to 20:455/455 of 8jdtA
- binding 2-ketobutyric acid: R318 (= R331), H369 (= H382), H376 (= H389), H414 (= H427)
- binding flavin-adenine dinucleotide: P68 (= P81), G70 (= G83), T71 (= T84), G72 (= G85), T73 (≠ S86), G74 (≠ S87), G78 (≠ H91), V79 (= V92), L90 (≠ M103), P132 (= P145), G133 (= G146), A134 (= A147), G140 (= G153), M141 (= M154), A143 (≠ S156), T144 (= T157), A146 (= A159), S147 (= S160), E200 (= E210), G201 (= G211), I206 (≠ V216), W322 (≠ Y335), E413 (= E426), H414 (= H427), N450 (= N462)
- binding manganese (ii) ion: H369 (= H382), H376 (= H389), E413 (= E426)
8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
48% identity, 93% coverage: 33:467/468 of query aligns to 20:455/455 of 8jdeA
- binding flavin-adenine dinucleotide: P68 (= P81), G70 (= G83), T71 (= T84), G72 (= G85), T73 (≠ S86), G74 (≠ S87), G78 (≠ H91), V79 (= V92), L90 (≠ M103), P132 (= P145), G133 (= G146), A134 (= A147), G140 (= G153), M141 (= M154), A143 (≠ S156), T144 (= T157), A146 (= A159), S147 (= S160), E200 (= E210), G201 (= G211), I206 (≠ V216), W322 (≠ Y335), E413 (= E426), H414 (= H427), N450 (= N462)
- binding lactic acid: R318 (= R331), H369 (= H382), H376 (= H389), H414 (= H427)
- binding manganese (ii) ion: H369 (= H382), H376 (= H389), E413 (= E426)
8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
49% identity, 93% coverage: 33:467/468 of query aligns to 20:455/455 of 8jduA
- binding 2-oxopentanoic acid: R318 (= R331), W322 (≠ Y335), H369 (= H382), H376 (= H389), H414 (= H427)
- binding flavin-adenine dinucleotide: P68 (= P81), G70 (= G83), T71 (= T84), G72 (= G85), T73 (≠ S86), G74 (≠ S87), G78 (≠ H91), V79 (= V92), L90 (≠ M103), P132 (= P145), G133 (= G146), A134 (= A147), G140 (= G153), M141 (= M154), A143 (≠ S156), T144 (= T157), A146 (= A159), S147 (= S160), E200 (= E210), G201 (= G211), I206 (≠ V216), W322 (≠ Y335), E413 (= E426), N450 (= N462)
- binding manganese (ii) ion: H369 (= H382), H376 (= H389), E413 (= E426)
8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
48% identity, 93% coverage: 33:467/468 of query aligns to 20:456/456 of 8jdrA
- binding flavin-adenine dinucleotide: P68 (= P81), G70 (= G83), T71 (= T84), G72 (= G85), T73 (≠ S86), G74 (≠ S87), G78 (≠ H91), V79 (= V92), L90 (≠ M103), P132 (= P145), G133 (= G146), A134 (= A147), G140 (= G153), M141 (= M154), A143 (≠ S156), T144 (= T157), A146 (= A159), S147 (= S160), E200 (= E210), G201 (= G211), I206 (≠ V216), Y324 (≠ W336), H370 (= H382), E414 (= E426), N451 (= N462)
- binding (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid: R319 (= R331), W323 (≠ Y335), H415 (= H427)
8jdqA Crystal structure of h405a mldhd in complex with d-2-hydroxyisocaproic acid (see paper)
48% identity, 93% coverage: 33:467/468 of query aligns to 20:456/456 of 8jdqA
- binding (2R)-2-hydroxy-4-methylpentanoic acid: R319 (= R331), W323 (≠ Y335), H370 (= H382), H415 (= H427)
- binding flavin-adenine dinucleotide: P68 (= P81), G70 (= G83), T71 (= T84), G72 (= G85), T73 (≠ S86), G74 (≠ S87), G78 (≠ H91), V79 (= V92), L90 (≠ M103), P132 (= P145), G133 (= G146), A134 (= A147), G140 (= G153), M141 (= M154), A143 (≠ S156), T144 (= T157), A146 (= A159), S147 (= S160), E200 (= E210), G201 (= G211), I206 (≠ V216), H370 (= H382), E414 (= E426), N451 (= N462)
8jdoA Crystal structure of h405a mldhd in complex with d-2-hydroxyhexanoic acid (see paper)
48% identity, 93% coverage: 33:467/468 of query aligns to 20:456/456 of 8jdoA
- binding (2R)-2-hydroxyhexanoic acid: R319 (= R331), W323 (≠ Y335), H415 (= H427)
- binding flavin-adenine dinucleotide: P68 (= P81), G70 (= G83), T71 (= T84), G72 (= G85), T73 (≠ S86), G74 (≠ S87), G78 (≠ H91), V79 (= V92), L90 (≠ M103), P132 (= P145), G133 (= G146), A134 (= A147), G140 (= G153), M141 (= M154), A143 (≠ S156), T144 (= T157), A146 (= A159), S147 (= S160), E200 (= E210), G201 (= G211), I206 (≠ V216), Y324 (≠ W336), H370 (= H382), E414 (= E426), N451 (= N462)
8jdnA Crystal structure of h405a mldhd in complex with d-2-hydroxyvaleric acid (see paper)
48% identity, 93% coverage: 33:467/468 of query aligns to 20:456/456 of 8jdnA
- binding flavin-adenine dinucleotide: P68 (= P81), G70 (= G83), T71 (= T84), G72 (= G85), T73 (≠ S86), G74 (≠ S87), G78 (≠ H91), V79 (= V92), L90 (≠ M103), P132 (= P145), G133 (= G146), A134 (= A147), G140 (= G153), M141 (= M154), A143 (≠ S156), T144 (= T157), A146 (= A159), S147 (= S160), E200 (= E210), G201 (= G211), I206 (≠ V216), H370 (= H382), E414 (= E426), N451 (= N462)
- binding (2R)-2-oxidanylpentanoic acid: R319 (= R331), W323 (≠ Y335), H415 (= H427)
8jdgA Crystal structure of h405a mldhd in complex with d-2-hydroxybutanoic acid (see paper)
48% identity, 93% coverage: 33:467/468 of query aligns to 20:456/456 of 8jdgA
- binding flavin-adenine dinucleotide: P68 (= P81), G70 (= G83), T71 (= T84), G72 (= G85), T73 (≠ S86), G74 (≠ S87), G78 (≠ H91), V79 (= V92), L90 (≠ M103), P132 (= P145), G133 (= G146), A134 (= A147), G140 (= G153), M141 (= M154), A143 (≠ S156), T144 (= T157), A146 (= A159), S147 (= S160), E200 (= E210), G201 (= G211), I206 (≠ V216), H370 (= H382), E414 (= E426), N451 (= N462)
- binding (2R)-2-oxidanylbutanoic acid: R319 (= R331), H415 (= H427)
8jdbA Crystal structure of h405a mldhd in complex with d-2-hydroxyoctanoic acid (see paper)
48% identity, 93% coverage: 33:467/468 of query aligns to 20:456/456 of 8jdbA
- binding flavin-adenine dinucleotide: P68 (= P81), G70 (= G83), T71 (= T84), G72 (= G85), T73 (≠ S86), G74 (≠ S87), G78 (≠ H91), V79 (= V92), L90 (≠ M103), P132 (= P145), G133 (= G146), A134 (= A147), G140 (= G153), M141 (= M154), A143 (≠ S156), T144 (= T157), A146 (= A159), S147 (= S160), E200 (= E210), G201 (= G211), I206 (≠ V216), Y324 (≠ W336), H370 (= H382), E414 (= E426), N451 (= N462)
- binding (2R)-2-oxidanyloctanoic acid: V75 (≠ L88), R319 (= R331), W323 (≠ Y335), H415 (= H427)
8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
48% identity, 93% coverage: 33:467/468 of query aligns to 20:455/455 of 8jdxA
- binding flavin-adenine dinucleotide: E32 (≠ T45), P68 (= P81), G70 (= G83), T71 (= T84), G72 (= G85), T73 (≠ S86), G74 (≠ S87), G78 (≠ H91), V79 (= V92), L90 (≠ M103), P132 (= P145), G133 (= G146), A134 (= A147), G140 (= G153), M141 (= M154), A143 (≠ S156), T144 (= T157), A146 (= A159), S147 (= S160), E200 (= E210), G201 (= G211), I206 (≠ V216), W322 (≠ Y335), E413 (= E426), H414 (= H427), N450 (= N462)
- binding 3-methyl-2-oxobutanoic acid: R318 (= R331), H369 (= H382), H376 (= H389), H414 (= H427)
- binding manganese (ii) ion: H369 (= H382), H376 (= H389), E413 (= E426)
8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
48% identity, 93% coverage: 33:467/468 of query aligns to 20:454/454 of 8jdvA
- binding 2-Ketohexanoic acid: V75 (≠ L88), R317 (= R331), W321 (≠ Y335), H368 (= H382), H375 (= H389), H413 (= H427)
- binding flavin-adenine dinucleotide: P68 (= P81), G70 (= G83), T71 (= T84), G72 (= G85), T73 (≠ S86), G74 (≠ S87), G78 (≠ H91), V79 (= V92), L90 (≠ M103), P132 (= P145), G133 (= G146), A134 (= A147), G140 (= G153), M141 (= M154), A143 (≠ S156), T144 (= T157), A146 (= A159), S147 (= S160), E200 (= E210), G201 (= G211), I206 (≠ V216), W321 (≠ Y335), Y322 (≠ W336), E412 (= E426), H413 (= H427), N449 (= N462)
- binding manganese (ii) ion: H368 (= H382), H375 (= H389), E412 (= E426)
8jdpA Crystal structure of h405a mldhd in complex with d-2-hydroxyisovaleric acid (see paper)
48% identity, 93% coverage: 33:467/468 of query aligns to 20:455/455 of 8jdpA
- binding flavin-adenine dinucleotide: P68 (= P81), G70 (= G83), T71 (= T84), G72 (= G85), T73 (≠ S86), G74 (≠ S87), G78 (≠ H91), V79 (= V92), L90 (≠ M103), P132 (= P145), G133 (= G146), A134 (= A147), G140 (= G153), M141 (= M154), A143 (≠ S156), T144 (= T157), A146 (= A159), S147 (= S160), E200 (= E210), G201 (= G211), I206 (≠ V216), H369 (= H382), E413 (= E426), N450 (= N462)
- binding deaminohydroxyvaline: R319 (= R331), H414 (= H427)
8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
48% identity, 93% coverage: 33:467/468 of query aligns to 20:454/454 of 8jdzA
- binding (3S)-3-methyl-2-oxopentanoic acid: R318 (= R331), W322 (≠ Y335), H369 (= H382), H376 (= H389), H413 (= H427)
- binding flavin-adenine dinucleotide: E32 (≠ T45), P68 (= P81), G70 (= G83), T71 (= T84), G72 (= G85), T73 (≠ S86), G74 (≠ S87), G78 (≠ H91), V79 (= V92), L90 (≠ M103), P132 (= P145), G133 (= G146), A134 (= A147), G140 (= G153), M141 (= M154), A143 (≠ S156), T144 (= T157), A146 (= A159), S147 (= S160), E200 (= E210), G201 (= G211), I206 (≠ V216), W322 (≠ Y335), E412 (= E426), H413 (= H427), N449 (= N462)
- binding manganese (ii) ion: H369 (= H382), H376 (= H389), E412 (= E426)
8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid (see paper)
48% identity, 93% coverage: 33:467/468 of query aligns to 20:454/454 of 8jdyA
- binding 2-oxo-4-methylpentanoic acid: R318 (= R331), W322 (≠ Y335), S336 (= S349), H369 (= H382), H376 (= H389), H413 (= H427)
- binding flavin-adenine dinucleotide: P68 (= P81), G70 (= G83), T71 (= T84), G72 (= G85), T73 (≠ S86), G74 (≠ S87), G78 (≠ H91), V79 (= V92), L90 (≠ M103), P132 (= P145), G133 (= G146), A134 (= A147), G140 (= G153), M141 (= M154), A143 (≠ S156), T144 (= T157), A146 (= A159), S147 (= S160), E200 (= E210), G201 (= G211), I206 (≠ V216), E412 (= E426), N449 (= N462)
- binding manganese (ii) ion: H369 (= H382), H376 (= H389), E412 (= E426)
7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
33% identity, 96% coverage: 16:466/468 of query aligns to 18:463/467 of 7qh2C
- binding flavin-adenine dinucleotide: V73 (≠ P81), G75 (= G83), S76 (≠ T84), G77 (= G85), T78 (≠ S86), G79 (≠ S87), L80 (= L88), A83 (≠ H91), C84 (≠ V92), P137 (= P145), G138 (= G146), E139 (vs. gap), A142 (= A149), T143 (≠ S150), G146 (= G153), N147 (≠ M154), S149 (= S156), T150 (= T157), A152 (= A159), G153 (≠ S160), E203 (= E210), G204 (= G211), I209 (≠ V216), E422 (= E426), H423 (= H427)
- binding fe (iii) ion: H377 (= H382), H384 (= H389), E422 (= E426)
P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
32% identity, 93% coverage: 33:467/468 of query aligns to 19:456/459 of P9WIT1
- K354 (≠ D361) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
31% identity, 88% coverage: 56:466/468 of query aligns to 44:464/465 of 3pm9A
- active site: A149 (= A159), L159 (≠ T169)
- binding flavin-adenine dinucleotide: P69 (= P81), Q70 (≠ F82), G71 (= G83), G72 (≠ T84), N73 (≠ G85), T74 (≠ S86), G75 (≠ S87), L76 (= L88), G79 (≠ H91), Q80 (≠ V92), L91 (≠ M103), L133 (≠ P145), G134 (= G146), A135 (= A147), C139 (vs. gap), T140 (vs. gap), G142 (= G152), G143 (= G153), S146 (= S156), T147 (= T157), A149 (= A159), G150 (≠ S160), E200 (= E210), G201 (= G211), I205 (= I215), I206 (≠ V216), E423 (= E426)
6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
27% identity, 87% coverage: 59:467/468 of query aligns to 54:465/467 of 6lpnB
- binding flavin-adenine dinucleotide: P76 (= P81), G78 (= G83), G79 (≠ T84), N80 (≠ G85), T81 (≠ S86), G82 (≠ S87), M83 (≠ L88), G86 (≠ H91), S87 (≠ V92), L140 (≠ P145), A142 (= A147), C146 (vs. gap), H147 (vs. gap), G150 (= G153), N151 (≠ M154), A153 (≠ S156), T154 (= T157), G208 (= G211), I212 (= I215), I213 (≠ V216), E423 (= E426), N460 (= N462)
Sites not aligning to the query:
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
27% identity, 87% coverage: 59:467/468 of query aligns to 106:517/521 of Q8N465
- S109 (≠ A62) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ I80) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (≠ T84) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ S100) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (= M106) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (≠ V125) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (= P142) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (≠ S156) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (= A182) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G184) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (≠ N320) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R331) binding ; binding ; binding ; mutation to A: Loss of catalytic activity.
- T390 (≠ Q338) binding ; binding ; mutation to A: Significantly reduced catalytic activity.
- V399 (≠ I347) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (≠ S349) binding ; binding ; mutation to A: Loss of catalytic activity.
- R419 (≠ H367) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- A426 (≠ G374) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- H434 (= H382) binding ; mutation to A: Loss of catalytic activity.
- G436 (= G384) to V: slight reduction in catalytic activity
- N439 (= N387) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- H441 (= H389) binding ; mutation to A: Loss of catalytic activity.
- N443 (≠ G391) binding ; mutation to A: Significantly reduced catalytic activity.
- V444 (≠ L392) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- A446 (≠ F394) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- E475 (= E426) binding ; mutation to A: Loss of catalytic activity.
- H476 (= H427) binding ; binding ; binding ; mutation to A: Loss of catalytic activity.
- G477 (= G428) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
Query Sequence
>SMc01455 FitnessBrowser__Smeli:SMc01455
MALNAIRAGARNENGIAAAKTYLATRFGDRFQTGEAIRAQHANTTTYIPAQLPDAVVFPE
SAAEVREIVEIACEHRVPLIPFGTGSSLEGHVNAPHGGISVDMMRMNRVLAVNAEDLDCT
VEPGVTREELNSYLRDTGLFFPIDPGANASIGGMASTRASGTNAVRYGTMKENVLAVTAV
VAGGREIRTAHRARKSSAGYDLTRLFVGAEGTLGIVTSITLRLQGIPEVISGGVCPFPTI
ADACNAVILTIQSGIPVARIELLDALQMKACNAYSGLTYQETPTLFVEFHGNGESVELQS
RQFAEIASEFGSTGFIWTTNPEERARLWKARHNAYWAQKSLMPGRAILSTDVCVPISRLA
DCVAATHEDIAAHGLVAPIVGHAGDGNFHVGLLFDDKDAADVAQAEAFVERLNARALSMD
GTCTGEHGIGQGKMPFLAAELGDALDLMRQIKRSLDPDNIFNPGKIFA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory