SitesBLAST

Q58989 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see 3 papers)
43% identity, 71% coverage: 77:286/295 of query aligns to 2:211/211 of Q58989

query
sites
Q58989

S

E

R

K

K

K

R

K

F

L

L

I

I

L

A

F

D
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D

M

F

D
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D

S

S

T

T

M

L

I

V

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E
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E

C

T

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I

D

D

E

E

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I

A

A

A

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A

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G

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E

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A

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N

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G

G

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D

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L

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P

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A

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I

A

-

T

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I

I

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G

A

R

E

E

E

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I

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K

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N

K

R

G

G

H

Y

Y

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S

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A

A

L

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V

V

S
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S

G
|
G

G

G

F

F

T

D

V

I

F

A

T

V

G

N

P

K

I

I

A

K

E

E

K

K

L

L

G

G

F

L

D

D

E

Y

N

A

R

F

A

A

N

N

I

R

L

L

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I

E

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E

K

N

D

G

G

K

K

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T

G

G

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D

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V

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R

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G

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K

E

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K

V

G

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D

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I

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E

D

D

V

T

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A

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D

G

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A

A

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N

D

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A

A

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C

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G

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K

Y

Y

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K

D11 (= D86) active site, Nucleophile; binding ; mutation to N: Loss of activity.
D13 (= D88) active site, Proton donor; binding
E20 (= E95) binding
R56 (= R131) binding
SG 99:100 (= SG 175:176) binding
K144 (= K220) binding
D167 (= D243) binding
N170 (= N246) binding

1f5sA Crystal structure of phosphoserine phosphatase from methanococcus jannaschii (see paper)
43% identity, 71% coverage: 77:286/295 of query aligns to 1:210/210 of 1f5sA

query
sites
1f5sA

S

E

R

K

K

K

R

K

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A

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D
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D

M
x
F

D
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D

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T

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G

N

Q

N

E

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C

T

I

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A

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E

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A

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N

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G

G

A

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A

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R

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G

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Y

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S

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G

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G

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F

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K

I

I

A

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E

E

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G

F

L

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Y

N

A

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F

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A

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N

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N

D

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D

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active site: D10 (= D86), F11 (≠ M87), D12 (= D88), G99 (= G176), K143 (= K220), D170 (= D247)
binding magnesium ion: D10 (= D86), D12 (= D88), D166 (= D243)
binding phosphate ion: D10 (= D86), F11 (≠ M87), D12 (= D88), S98 (= S175), G99 (= G176), K143 (= K220)

1l7nA Transition state analogue of phosphoserine phosphatase (aluminum fluoride complex) (see paper)
43% identity, 71% coverage: 78:286/295 of query aligns to 1:209/209 of 1l7nA

query
sites
1l7nA

R

K

K

K

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x
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N

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G
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G

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I

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A

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K

V

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E

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D

L

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A

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active site: D9 (= D86), F10 (≠ M87), D11 (= D88), G98 (= G176), K142 (= K220), D169 (= D247)
binding aluminum fluoride: D9 (= D86), F10 (≠ M87), D11 (= D88), S97 (= S175), K142 (= K220)
binding tetrafluoroaluminate ion: D9 (= D86), F10 (≠ M87), D11 (= D88), S97 (= S175), G98 (= G176), K142 (= K220), N168 (= N246)
binding magnesium ion: D9 (= D86), D11 (= D88), D165 (= D243)

1l7pA Substrate bound phosphoserine phosphatase complex structure (see paper)
43% identity, 71% coverage: 79:286/295 of query aligns to 1:208/208 of 1l7pA

query
sites
1l7pA

R

K

K

K

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E

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G

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A

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L

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D

D

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A

A

N

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D

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A

A

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H

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G

R

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Y

Y

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active site: N8 (≠ D86), F9 (≠ M87), D10 (= D88), G97 (= G176), K141 (= K220), D168 (= D247)
binding phosphoserine: N8 (≠ D86), F9 (≠ M87), D10 (= D88), E17 (= E95), M40 (= M118), F46 (= F124), R53 (= R131), S96 (= S175), G97 (= G176), K141 (= K220)

8a21A Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with phenylimidazole (see paper)
43% identity, 76% coverage: 68:290/295 of query aligns to 163:386/396 of 8a21A

query
sites
8a21A

I

V

D

D

V

V

A

A

V

V

Q

E

D

D

A

Y

-

T

-

L

E

E

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R

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R

R

A

K

K

R

R

F

L

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I

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D

M

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D
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D

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E
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E

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I
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I

D

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E

M

L

L

A

A

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K

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G

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E

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A

A

I

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T

A

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M

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F

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R

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A

A

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A

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G

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P

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E

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V

I

-

A

A

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L

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P

G

G

G

A

R

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E

T

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T

I

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A

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K

R

A

R

K

L

G

G

H

Y

Y

A

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C

A

G

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S

G

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G
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G

F

F

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R

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P

I

L

A

A

E

E

K

E

L

L

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M

F

L

D

D

E

Y

N

V

R

A

A

A

N

N

I

E

L

L

V

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E

I

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V

N

D

G

G

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T

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L

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R

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A

A

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A

A

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L

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R

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E

I

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Q

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R

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A

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G

I

V

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P

P

M

A

A

D

Q

V

T

I

V

A

A

V

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G

G

D
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D

G

G

A

A

N

N

D

D

L

I

G

D

M

M

L

L

Q

A

L

A

A

A

G

G

T

L

G

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I

A

A

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F

H

N

A

A

K

K

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P

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A

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R

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E

Q

V

A

A

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I

A

R

S

I

L

D

S

H

H

G

P

D

Y

L

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T

D

A

T

L

V

L

L

Y

F

L

L

Q

L

G

G

Y

V

R

T

K

R

binding magnesium ion: D183 (= D86), D185 (= D88), D339 (= D243)
binding 4-phenyl-1h-imidazole: D185 (= D88), E192 (= E95), V193 (≠ C96), I194 (= I97), T211 (= T114), M215 (= M118), F221 (= F124), R228 (= R131), G273 (= G177)

Sites not aligning to the query:

binding 4-phenyl-1h-imidazole: 13, 18, 37

8a1zA Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with 1-(2,4-dichlorophenyl)-3-hydroxyurea (see paper)
43% identity, 76% coverage: 68:290/295 of query aligns to 163:386/396 of 8a1zA

query
sites
8a1zA

I

V

D

D

V

V

A

A

V

V

Q

E

D

D

A

Y

-

T

-

L

E

E

S

R

R

R

R

A

K

K

R

R

F

L

L

I

I

V

A

F

D
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D

M

V

D
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D

S

S

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M

L

I

V

G

Q

Q

G

E
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E

C

V

I

I

D

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E

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L

L

A

A

E

K

V

A

G

G

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A

K

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E

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V

A

A

I

I

T

T

A

D

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A

A

A

M
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M

N

R

G

G

E

E

I

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A

D

F
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F

E

A

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A

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L
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L

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R

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V

A

A

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L

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A

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G

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P

V

A

T

T

V

V

V

I

A

D

E

E

V

V

I

-

A

A

T

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R

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I

L

T

E

L

L

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M

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P

G

G

G

A

R

R

E

T

L

T

I

L

A

R

T

T

M

L

K

R

A

R

K

L

G

G

H

Y

Y

A

S

C

A

G

L

V

V

V

S

S

G
|
G

G

G

F
|
F

T

R

V

R

F

I

T

I

G

E

P

P

I

L

A

A

E

E

K

E

L

L

G

M

F

L

D

D

E

Y

N

V

R

A

A

A

N

N

I

E

L

L

V

E

E

I

E

V

N

D

G

G

K

T

L

L

T

T

G

G

D

R

V

V

A

V

R

G

P

P

I

I

L

I

G

D

K

R

Q

A

A

G

K

K

V

A

D

T

A

A

L

L

I

R

D

E

I

F

S

A

E

Q

R

R

L

A

G

G

I

V

T

P

P

M

A

A

D

Q

V

T

I

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D

D

L

I

G

D

M

M

L

L

Q

A

L

A

A

A

G

G

T

L

G

G

V

I

A

A

L

F

H

N

A

A

K

K

P

P

V

A

V

L

A

R

D

E

Q

V

A

A

K

D

I

A

R

S

I

L

D

S

H

H

G

P

D

Y

L

L

T

D

A

T

L

V

L

L

Y

F

L

L

Q

L

G

G

Y

V

R

T

K

R

binding 1-(2,4-dichlorophenyl)-3-oxidanyl-urea: D185 (= D88), E192 (= E95), M215 (= M118), F221 (= F124), L225 (= L128), R228 (= R131), G272 (= G176), F274 (= F178), D339 (= D243)
binding magnesium ion: D183 (= D86), D185 (= D88), D339 (= D243)

5jlpA Crystal structure of mycobacterium avium serb2 in complex with serine at act domain
43% identity, 76% coverage: 68:290/295 of query aligns to 163:386/396 of 5jlpA

query
sites
5jlpA

I

V

D

D

V

V

A

A

V

V

Q

E

D

D

A

Y

-

T

-

L

E

E

S

R

R

R

R

A

K

K

R

R

F

L

L

I

I

V

A

F

D
|
D

M
x
V

D
|
D

S

S

T

T

M

L

I

V

G

Q

Q

G

E

E

C

V

I

I

D

E

E

M

L

L

A

A

E

K

V

A

G

G

L

A

K

E

E

G

K

Q

V

V

A

A

I

I

T

T

A

D

R

A

A

A

M

M

N

R

G

G

E

E

I

L

A

D

F

F

E

A

P

Q

A

S

L

L

V

Q

E

Q

R

R

V

V

A

A

L

T

L

L

Q

A

G

G

L

L

P

P

V

A

T

T

V

V

V

I

A

D

E

E

V

V

I

-

A

A

T

G

R

Q

I

L

T

E

L

L

T

M

P

P

G

G

G

A

R

R

E

T

L

T

I

L

A

R

T

T

M

L

K

R

A

R

K

L

G

G

H

Y

Y

A

S

C

A

G

L

V

V

V

S

S

G
|
G

G

G

F

F

T

R

V

R

F

I

T

I

G

E

P

P

I

L

A

A

E

E

K

E

L

L

G

M

F

L

D

D

E

Y

N

V

R

A

A

A

N

N

I

E

L

L

V

E

E

I

E

V

N

D

G

G

K

T

L

L

T

T

G

G

D

R

V

V

A

V

R

G

P

P

I

I

L

I

G

D

K

R

Q

A

A

G

K
|
K

V

A

D

T

A

A

L

L

I

R

D

E

I

F

S

A

E

Q

R

R

L

A

G

G

I

V

T

P

P

M

A

A

D

Q

V

T

I

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

I

G

D

M

M

L

L

Q

A

L

A

A

A

G

G

T

L

G

G

V

I

A

A

L

F

H

N

A

A

K

K

P

P

V

A

V

L

A

R

D

E

Q

V

A

A

K

D

I

A

R

S

I

L

D

S

H

H

G

P

D

Y

L

L

T

D

A

T

L

V

L

L

Y

F

L

L

Q

L

G

G

Y

V

R

T

K

R

active site: D183 (= D86), V184 (≠ M87), D185 (= D88), G272 (= G176), K316 (= K220), D343 (= D247)
binding magnesium ion: D183 (= D86), D339 (= D243)

Sites not aligning to the query:

binding serine: 13, 14

A0QJI1 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Mycobacterium avium (strain 104) (see paper)
43% identity, 76% coverage: 68:290/295 of query aligns to 167:390/411 of A0QJI1

query
sites
A0QJI1

I

V

D

D

V

V

A

A

V

V

Q

E

D

D

A

Y

-

T

-

L

E

E

S

R

R

R

R

A

K

K

R

R

F

L

L

I

I

V

A

F

D
|
D

M

V

D
|
D

S

S

T

T

M

L

I

V

G

Q

Q

G

E

E

C

V

I

I

D

E

E

M

L

L

A

A

E

K

V

A

G

G

L

A

K

E

E

G

K

Q

V

V

A

A

I

I

T

T

A

D

R

A

A

A

M

M

N

R

G

G

E

E

I

L

A

D

F

F

E

A

P

Q

A

S

L

L

V

Q

E

Q

R

R

V

V

A

A

L

T

L

L

Q

A

G

G

L

L

P

P

V

A

T

T

V

V

V

I

A

D

E

E

V

V

I

-

A

A

T

G

R

Q

I

L

T

E

L

L

T

M

P

P

G

G

G

A

R

R

E

T

L

T

I

L

A

R

T

T

M

L

K

R

A

R

K

L

G

G

H

Y

Y

A

S

C

A

G

L

V

V

V

S

S

G

G

F

F

T

R

V

R

F

I

T

I

G

E

P

P

I

L

A

A

E

E

K

E

L

L

G

M

F

L

D

D

E

Y

N

V

R

A

A

A

N

N

I

E

L

L

V

E

E

I

E

V

N

D

G

G

K

T

L

L

T

T

G

G

D

R

V

V

A

V

R

G

P

P

I

I

L

I

G

D

K

R

Q

A

A

G

K

K

V

A

D

T

A

A

L

L

I

R

D

E

I

F

S

A

E

Q

R

R

L

A

G

G

I

V

T

P

P

M

A

A

D

Q

V

T

I

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D

D

L

I

G

D

M

M

L

L

Q

A

L

A

A

A

G

G

T

L

G

G

V

I

A

A

L

F

H

N

A

A

K

K

P

P

V

A

V

L

A

R

D

E

Q

V

A

A

K

D

I

A

R

S

I

L

D

S

H

H

G

P

D

Y

L

L

T

D

A

T

L

V

L

L

Y

F

L

L

Q

L

G

G

Y

V

R

T

K

R

D187 (= D86) binding
D189 (= D88) binding
D343 (= D243) binding

1l7oA Crystal structure of phosphoserine phosphatase in apo form (see paper)
42% identity, 71% coverage: 79:286/295 of query aligns to 1:200/200 of 1l7oA

query
sites
1l7oA

R

K

K

K

R

K

F

L

L

I

I

L

A

F

D

N

M

F

D

D

S

S

T

T

M

L

I

V

G

N

Q

N

E

E

C

T

I

I

D

D

E

E

L

I

A

A

A

R

E

E

V

A

G

G

L

V

K

E

E

E

K

E

V

V

A

K

I

I

T

T

A

N

R

-

A

-

M

-

N

-

G

-

E

-

I

-

A

-

F

F

E

E

P

Q

A

S

L

L

V

R

E

K

R

R

V

V

A

S

L

L

Q

K

G

D

L

L

P

P

V

I

T

E

V

K

V

V

A

E

E

K

V

A

I

I

A

-

T

K

R

R

I

I

T

T

L

P

T

T

P

E

G

G

G

A

R

E

E

E

L

T

I

I

A

K

T

E

M

L

K

K

A

N

K

R

G

G

H

Y

Y

V

S

V

A

A

L

V

V

V

S

S

G

G

F

F

T

D

V

I

F

A

T

V

G

N

P

K

I

I

A

K

E

E

K

K

L

L

G

G

F

L

D

D

E

Y

N

A

R

F

A

A

N

N

I

R

L

L

V

I

E

V

E

K

N

D

G

G

K

K

L

L

T

T

G

G

D

D

V

V

A

E

R

G

P

E

I

V

L

L

G

K

K

E

Q

N

A

A

K

K

V

G

D

E

A

I

L

L

I

E

D

K

I

I

S

A

E

K

R

I

L

E

G

G

I

I

T

N

P

L

A

E

D

D

V

T

I

V

A

A

V

V

G

G

D

D

G

G

A

A

N

N

D

D

L

I

G

S

M

M

L

F

Q

K

L

K

A

A

G

G

T

L

G

K

V

I

A

A

L

F

H

C

A

A

K

K

P

P

V

I

V

L

A

K

D

E

Q

K

A

A

K

D

I

I

R
x
C

I

I

D
x
E

H

K

G

R

D

D

L

L

T

R

A

E

L

I

L

L

-

K

Y

Y

L

I

Q

K

binding zinc ion: C186 (≠ R273), E188 (≠ D275)

O53289 Phosphoserine phosphatase SerB2; PSP; PSPase; O-phosphoserine phosphohydrolase; Protein-serine/threonine phosphatase; EC 3.1.3.3; EC 3.1.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
40% identity, 81% coverage: 53:290/295 of query aligns to 150:388/409 of O53289

query
sites
O53289

G

G

E

P

A

L

E

Q

A

I

R

A

L

L

R

T

Q

K

A

V

V

A

A

A

G

E

A

E

A

H

I

V

D

D

V

V

A

A

V

V

Q

E

D

D

A

Y

-

G

-

L

E

A

S

W

R

R

R

T

K

K

R

R

F

L

L

I

I

V

A

F

D
|
D

M
x
V

D
|
D

S
|
S

T

T

M

L

I

V

G

Q

Q

G

E

E

C

V

I

I

D

E

E

M

L

L

A

A

E

R

V

A

G

G

L

A

K

Q

E

G

K

Q

V

V

A

A

I

I

T

T

A

E

R

A

A

A

M

M

N

R

G

G

E

E

I

L

A

D

F

F

E

A

P

E

A

S

L

L

V

Q

E

R

R

R

V

V

A

A

L

T

L

L

Q

A

G

G

L

L

P

P

V

A

T

T

V

V

V

I

A

D

E

D

V

V

I

-

A

A

T

E

R

Q

I

L

T

E

L

L

T

M

P

P

G

G

G

A

R

R

E

T

L

T

I

I

A

R

T

T

M

L

K

R

A

R

K

L

G

G

H

F

Y

R

S

C

A

G

L

V

V

V

S
|
S

G

G

F

F

T

R

V

R

F

I

T

I

G

E

P

P

I

L

A

A

E

R

K

E

L

L

G

M

F

L

D

D

E

F

N

V

R

A

A

S

N

N

I

E

L

L

V

E

E

I

E

V

N

D

G

G

K

I

L

L

T

T

G

G

D

R

V

V

A

V

R

G

P

P

I

I

L

V

G

D

K

R

Q

P

A

G

K
|
K

V

A

D

K

A

A

L

L

I

R

D

D

I

F

S

A

E

S

R

Q

L

Y

G

G

I

V

T

P

P

M

A

E

D

Q

V

T

I

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

I

G

D

M

M

L

L

Q

G

L

A

A

A

G

G

T

L

G

G

V

I

A

A

L

F

H

N

A

A

K

K

P

P

V

A

V

L

A

R

D

E

Q

V

A

A

K

D

I

A

R

S

I

L

D

S

H

H

G

P

D

Y

L

L

T

D

A

T

L

V

L

L

Y

F

L

L

Q

L

G

G

Y

V

R

T

K

R

D185 (= D86) mutation to G: Completely abolishes enzymatic activity.; mutation to N: Completely abolishes enzymatic activity.
V186 (≠ M87) mutation to Q: Decreases enzymatic activity by 50%.
D187 (= D88) mutation to N: Decreases enzymatic activity by 15%.
S188 (= S89) mutation to A: No effect on enzymatic activity.
S273 (= S175) mutation to A: Completely abolishes enzymatic activity (PubMed:25521849). Decreases enzymatic activity by 60% (PubMed:25037224).
K318 (= K220) mutation to A: Decreases enzymatic activity by 50%.; mutation to E: Completely abolishes enzymatic activity.
D341 (= D243) mutation to G: Decreases enzymatic activity by 80%.; mutation to N: Decreases enzymatic activity by 85%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-345.
D345 (= D247) mutation to N: Decreases enzymatic activity by 55%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-341.

Sites not aligning to the query:

18 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
108 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.

3m1yC Crystal structure of a phosphoserine phosphatase (serb) from helicobacter pylori
33% identity, 68% coverage: 80:279/295 of query aligns to 3:200/208 of 3m1yC

query
sites
3m1yC

K

Q

R

K

F

L

L

A

I

V

A

F

D
|
D

M
x
F

D
|
D

S

S

T

T

M

L

I

V

G

N

Q

A

E

E

C

T

I

I

D

E

E

S

L

L

A

A

A

R

E

A

V

W

G

G

L

V

K

F

E

D

K

E

V

V

A

K

A

T

I

I

T

T

A

L

R

K

A

A

M

M

N

N

G

G

E

-

I

T

A

D

F

F

E

H

P

K

A

S

L

L

V

I

E

L

R

R

V

V

A

S

L

K

L

L

Q

K

G

N

L

M

P

P

V

L

T

K

V

L

V

A

A

K

E

E

V

V

I

C

A

E

T

S

R

-

I

L

T

P

L

L

T

F

P

E

G

G

G

A

R

L

E

E

L

L

I

V

A

S

T

A

M

L

K

K

A

E

K

K

G

N

H

Y

Y

K

S

V

A

V

L

C

V

F

S

S

G
|
G

G

G

F

F

T

D

V

L

F

A

T

T

G

N

P

H

I

Y

A

R

E

D

K

L

L

L

G

H

F

L

D

D

E

A

N

A

R

F

A

S

N

N

I

T

L

L

V

I

E

V

E

E

N

N

G

D

K

A

L

L

T

N

G

G

D

L

V

V

A

T

R

G

P

H

I

M

L

M

G

F

K

S

Q

H

A

S

K
|
K

V

G

D

E

A

M

L

L

I

L

D

V

I

L

S

Q

E

R

R

L

L

L

G

N

I

I

T

S

P

K

A

T

D

N

V

T

I

L

A

V

V

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

L

G

S

M

M

L

F

Q

K

L

H

A

A

G

H

T

I

G

K

V

I

A

A

L

F

H

N

A

A

K

K

P

E

V

V

V

L

A

K

D

Q

Q

H

A

A

K

T

I

H

R

C

I

I

D

N

H

E

G

P

D

D

L

L

active site: D9 (= D86), F10 (≠ M87), D11 (= D88), G97 (= G176), K141 (= K220), D168 (= D247)
binding magnesium ion: D9 (= D86), D11 (= D88), D164 (= D243)

1l8oA Molecular basis for the local conformational rearrangement of human phosphoserine phosphatase (see paper)
34% identity, 58% coverage: 79:248/295 of query aligns to 5:181/222 of 1l8oA

query
sites
1l8oA

R

R

K

K

R

L

F

F

L

Y

I

S

A

A

D

D

-

A

-

V

-

C

-

F

-
x
D

M
x
V

D
|
D

S

S

T

T

M

V

I

I

G

R

Q

E

E

E

C

G

I

I

D

D

E

E

L

L

A

A

A

K

E

I

V

C

G

G

L

V

K

E

E

D

K

A

V

V

A

S

A

E

I

M

T

T

A

R

R

R

A

A

M

M

N

G

G

G

E

A

I

V

A

P

F

F

E

K

P

A

A

A

L

L

V

T

E

E

R

R

V

L

A

A

L

L

L

I

Q

Q

G

P

L

S

P

R

V

E

T

Q

V

V

V

Q

A

R

E

L

V

I

I

A

A

E

T

Q

R

P

I

P

T

H

L

L

T

T

P

P

G

G

G

I

R

R

E

E

L

L

I

V

A

S

T

R

M

L

K

Q

A

E

K

R

G

N

H

V

Y

Q

S

V

A

F

L

L

V

I

S
|
S

G
|
G

G

G

F

F

T

R

V

S

F

I

T

V

G

E

P

H

I

V

A

A

E

S

K

K

L

L

G

N

F

I

D

P

-

A

-

T

-

N

-

V

-

F

E

A

N

N

R

R

A

L

N

K

I

F

L

Y

V

F

E

-

N

N

G

G

K

E

L

Y

T

A

G

G

-

F

D

D

V

E

A

T

R

Q

P

P

I

T

L

A

G

E

K

S

Q

G

A

G

K
|
K

V

G

D

K

A

V

L

I

I

K

D

F

I

L

S

K

E

E

R

K

L

F

G

H

I

F

T

K

P

-

A

-

D

K

V

I

I

I

A

M

V

I

G

G

D

D

G
|
G

A

A

N
x
T

D
|
D

L

M

active site: D17 (vs. gap), V18 (≠ M87), D19 (= D88), G107 (= G176), K155 (= K220), D180 (= D247)
binding phosphate ion: D17 (vs. gap), D19 (= D88), S106 (= S175), K155 (= K220)
binding serine: G177 (= G244), T179 (≠ N246)

Sites not aligning to the query:

binding serine: 199

1l8lA Molecular basis for the local confomational rearrangement of human phosphoserine phosphatase (see paper)
34% identity, 58% coverage: 79:248/295 of query aligns to 5:181/222 of 1l8lA

query
sites
1l8lA

R

R

K

K

R

L

F

F

L

Y

I

S

A

A

D

D

-

A

-

V

-

C

-

F

-
x
D

M
x
V

D
|
D

S

S

T

T

M

V

I

I

G

R

Q

E

E

E

C

G

I

I

D

D

E

E

L

L

A

A

A

K

E

I

V

C

G

G

L

V

K

E

E

D

K

A

V

V

A

S

A

E

I

M

T

T

A

R

R

R

A

A

M

M

N

G

G

G

E

A

I

V

A

P

F

F

E

K

P

A

A

A

L

L

V

T

E

E

R

R

V

L

A

A

L

L

L

I

Q

Q

G

P

L

S

P

R

V

E

T

Q

V

V

V

Q

A

R

E

L

V

I

I

A

A

E

T

Q

R

P

I

P

T

H

L

L

T

T

P

P

G

G

G

I

R

R

E

E

L

L

I

V

A

S

T

R

M

L

K

Q

A

E

K

R

G

N

H

V

Y

Q

S

V

A

F

L

L

V

I

S

S

G
|
G

G

G

F

F

T

R

V

S

F

I

T

V

G

E

P

H

I

V

A

A

E

S

K

K

L

L

G

N

F

I

D

P

-

A

-

T

-

N

-

V

-

F

E

A

N

N

R

R

A

L

N

K

I

F

L

Y

V

F

E

-

N

N

G

G

K

E

L

Y

T

A

G

G

-

F

D

D

V

E

A

T

R

Q

P

P

I

T

L

A

G

E

K

S

Q

G

A

G

K
|
K

V

G

D

K

A

V

L

I

I

K

D

F

I

L

S

K

E

E

R

K

L

F

G

H

I

F

T

K

P

-

A

-

D

K

V

I

I

I

A

M

V

I

G

G

D
|
D

G
|
G

A

A

N
x
T

D
|
D

L

M

active site: D17 (vs. gap), V18 (≠ M87), D19 (= D88), G107 (= G176), K155 (= K220), D180 (= D247)
binding d-2-amino-3-phosphono-propionic acid: D17 (vs. gap), D19 (= D88), G107 (= G176), K155 (= K220), D176 (= D243), G177 (= G244), T179 (≠ N246)

P78330 Phosphoserine phosphatase; PSP; PSPase; L-3-phosphoserine phosphatase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Homo sapiens (Human) (see 4 papers)
34% identity, 58% coverage: 79:248/295 of query aligns to 8:184/225 of P78330

query
sites
P78330

R

R

K

K

R

L

F

F

L

Y

I

S

A

A

D

D

-

A

-

V

-

C

-

F

-
x
D

M
x
V

D
|
D

S
|
S

T

T

M

V

I

I

G

R

Q

E

E
|
E

C

G

I

I

D
|
D

E

E

L

L

A
|
A

A

K

E

I

V

C

G

G

L

V

K

E

E

D

K

A

V

V

A

S

A

E

I

M

T

T

A

R

R

R

A

A

M
|
M

N
x
G

G

G

E

A

I

V

A

P

F

F

E

K

P

A

A

A

L

L

V

T

E

E

R
|
R

V

L

A

A

L

L

L

I

Q

Q

G

P

L

S

P

R

V

E

T

Q

V

V

V

Q

A

R

E

L

V

I

I

A

A

E

T

Q

R

P

I

P

T

H

L

L

T

T

P

P

G

G

G

I

R

R

E

E

L

L

I

V

A

S

T

R

M

L

K

Q

A

E

K

R

G

N

H

V

Y

Q

S

V

A

F

L

L

V

I

S
|
S

G
|
G

F

F

T

R

V

S

F

I

T

V

G

E

P

H

I

V

A

A

E

S

K

K

L

L

G

N

F

I

D

P

-

A

-

T

-

N

-

V

-

F

E

A

N
|
N

R

R

A

L

N

K

I

F

L

Y

V

F

E

-

N

N

G

G

K

E

L

Y

T

A

G

G

-

F

D

D

V

E

A

T

R

Q

P

P

I

T

L

A

G

E

K

S

Q

G

A

G

K
|
K

V

G

D

K

A

V

L

I

I

K

D

L

I

L

S

K

E

E

R

K

L

F

G

H

I

F

T

K

P

-

A

-

D

K

V

I

I

I

A

M

V

I

G

G

D
|
D

G

G

A

A

N
x
T

D

D

L

M

D20 (vs. gap) binding
DVD 20:22 (≠ -MD 87:88) binding
D22 (= D88) binding
S23 (= S89) mutation to A: Reduces L-phosphoserine phosphatase activity by about 50%.; mutation to T: Reduces L-phosphoserine phosphatase activity by about 80%.
E29 (= E95) mutation to D: Reduces L-phosphoserine phosphatase activity by about 95%.; mutation to Q: Loss of L-phosphoserine phosphatase activity.
D32 (= D98) to N: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894035
A35 (= A101) to T: in PSPHD; decreased L-phosphoserine phosphatase activity
M52 (= M118) binding ; to T: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894036
G53 (≠ N119) binding
R65 (= R131) mutation R->A,K: Loss of L-phosphoserine phosphatase activity.
SGG 109:111 (= SGG 175:177) binding ; binding
N133 (= N194) mutation to A: Reduces L-phosphoserine phosphatase activity by about 75%.
K158 (= K220) binding ; binding
D179 (= D243) binding
T182 (≠ N246) binding ; binding ; mutation to S: Reduces L-phosphoserine phosphatase activity by about 99%.; mutation to V: Reduces L-phosphoserine phosphatase activity by about 25%.

Sites not aligning to the query:

202 R→A: Reduces L-phosphoserine phosphatase activity by about 99%.; R→K: Reduces L-phosphoserine phosphatase activity by about 95%.

6hyjB Psph human phosphoserine phosphatase (see paper)
34% identity, 58% coverage: 79:248/295 of query aligns to 8:184/223 of 6hyjB

query
sites
6hyjB

R

R

K

K

R

L

F

F

L

Y

I

S

A

A

D

D

-

A

-

V

-

C

-

F

-
x
D

M

V

D
|
D

S

S

T

T

M

V

I

I

G

R

Q

E

E
|
E

C

G

I

I

D

D

E

E

L

L

A

A

A

K

E

I

V

C

G

G

L

V

K

E

E

D

K

A

V

V

A

S

A

E

I

M

T

T

A

R

R

R

A

A

M

M

N

G

G

G

E

A

I

V

A

P

F

F

E

K

P

A

A

A

L

L

V

T

E

E

R

R

V

L

A

A

L

L

L

I

Q

Q

G

P

L

S

P

R

V

E

T

Q

V

V

V

Q

A

R

E

L

V

I

I

A

A

E

T

Q

R

P

I

P

T

H

L

L

T

T

P

P

G

G

G

I

R

R

E

E

L

L

I

V

A

S

T

R

M

L

K

Q

A

E

K

R

G

N

H

V

Y

Q

S

V

A

F

L

L

V

I

S

S

G

G

F

F

T

R

V

S

F

I

T

V

G

E

P

H

I

V

A

A

E

S

K

K

L

L

G

N

F

I

D

P

-

A

-

T

-

N

-

V

-
x
F

E

A

N

N

R

R

A

L

N

K

I

F

L

Y

V

F

E

-

N

N

G

G

K

E

L

Y

T

A

G

G

-

F

D

D

V

E

A

T

R

Q

P

P

I

T

L

A

G

E

K

S

Q

G

A

G

K

K

V

G

D

K

A

V

L

I

I

K

D

L

I

L

S

K

E

E

R
x
K

L

F

G

H

I

F

T

K

P

-

A

-

D

K

V

I

I

I

A

M

V

I

G

G

D
|
D

G

G

A

A

N

T

D

D

L

M

binding calcium ion: D20 (vs. gap), D22 (= D88), D179 (= D243)
binding phosphoserine: F131 (vs. gap), K168 (≠ R230)
binding serine: E29 (= E95)

Sites not aligning to the query:

binding serine: 202

6q6jB Human phosphoserine phosphatase with substrate analogue homo-cysteic acid (see paper)
34% identity, 55% coverage: 86:248/295 of query aligns to 16:180/217 of 6q6jB

query
sites
6q6jB

D
|
D

M
x
V

D
|
D

S

S

T

T

M

V

I

I

G

R

Q

E

E

E

C

G

I

I

D

D

E

E

L

L

A

A

A

K

E

I

V

C

G

G

L

V

K

E

E

D

K

A

V

V

A

S

A

E

I

M

T

T

A

R

R

R

A

A

M

M

N

G

G

G

E

A

I

V

A

P

F
|
F

E

K

P

A

A

A

L

L

V

T

E

E

R

R

V

L

A

A

L

L

L

I

Q

Q

G

P

L

S

P

R

V

E

T

Q

V

V

V

Q

A

R

E

L

V

I

I

A

A

E

T

Q

R

P

I

P

T

H

L

L

T

T

P

P

G

G

G

I

R

R

E

E

L

L

I

V

A

S

T

R

M

L

K

Q

A

E

K

R

G

N

H

V

Y

Q

S

V

A

F

L

L

V

I

S
|
S

G
|
G

F

F

T

R

V

S

F

I

T

V

G

E

P

H

I

V

A

A

E

S

K

K

L

L

G

N

F

I

D

P

-

A

-

T

-

N

-

V

-

F

E

A

N

N

R

R

A

L

N

K

I

F

L

Y

V

F

E

-

N

N

G

G

K

E

L

Y

T

A

G

G

-

F

D

D

V

E

A

T

R

Q

P

P

I

T

L

A

G

E

K

S

Q

G

A

G

K
|
K

V

G

D

K

A

V

L

I

I

K

D

L

I

L

S

K

E

E

R

K

L

F

G

H

I

F

T

K

P

-

A

-

D

K

V

I

I

I

A

M

V

I

G

G

D
|
D

G

G

A

A

N
x
T

D

D

L

M

binding calcium ion: D16 (= D86), D18 (= D88), D175 (= D243)
binding (2~{S})-2-azanyl-4-sulfo-butanoic acid: D16 (= D86), V17 (≠ M87), D18 (= D88), F54 (= F124), S105 (= S175), G106 (= G176), G107 (= G177), K154 (= K220), T178 (≠ N246)

6hyyA Human phosphoserine phosphatase with serine and phosphate (see paper)
34% identity, 58% coverage: 79:248/295 of query aligns to 4:180/221 of 6hyyA

query
sites
6hyyA

R

R

K

K

R

L

F

F

L

Y

I

S

A

A

D

D

-

A

-

V

-

C

-

F

-
x
D

M
x
V

D
|
D

S

S

T

T

M

V

I

I

G

R

Q

E

E

E

C

G

I

I

D

D

E

E

L

L

A

A

A

K

E

I

V

C

G

G

L

V

K

E

E

D

K

A

V

V

A

S

A

E

I

M

T

T

A

R

R

R

A

A

M

M

N

G

G

G

E

A

I

V

A

P

F

F

E

K

P

A

A

A

L

L

V

T

E

E

R

R

V

L

A

A

L

L

L

I

Q

Q

G

P

L

S

P

R

V

E

T

Q

V

V

V

Q

A

R

E

L

V

I

I

A

A

E

T

Q

R

P

I

P

T

H

L

L

T

T

P

P

G

G

G

I

R

R

E

E

L

L

I

V

A

S

T

R

M

L

K

Q

A

E

K

R

G

N

H

V

Y

Q

S

V

A

F

L

L

V

I

S
|
S

G
|
G

G

G

F

F

T

R

V

S

F

I

T

V

G

E

P

H

I

V

A

A

E

S

K

K

L

L

G

N

F

I

D

P

-

A

-

T

-

N

-

V

-

F

E

A

N

N

R

R

A

L

N

K

I

F

L

Y

V

F

E

-

N

N

G

G

K

E

L

Y

T

A

G

G

-

F

D

D

V

E

A

T

R

Q

P

P

I

T

L

A

G

E

K

S

Q

G

A

G

K
|
K

V

G

D

K

A

V

L

I

I

K

D

L

I

L

S

K

E

E

R

K

L

F

G

H

I

F

T

K

P

-

A

-

D

K

V

I

I

I

A

M

V

I

G

G

D
|
D

G

G

A

A

N

T

D

D

L

M

binding magnesium ion: D16 (vs. gap), D18 (= D88), D175 (= D243)
binding serine: D16 (vs. gap), V17 (≠ M87), D18 (= D88), S105 (= S175), G106 (= G176), K154 (= K220)

6iuyA Structure of dsgpdh of dunaliella salina (see paper)
28% identity, 63% coverage: 86:270/295 of query aligns to 20:209/585 of 6iuyA

query
sites
6iuyA

D
|
D

M

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D
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D

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T

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D

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E

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E

D

K

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A

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A

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E

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A

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M

N

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R

G

G

E

E

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I

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F

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K

A

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A

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N

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-

R

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binding magnesium ion: D20 (= D86), D22 (= D88), D175 (= D243)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 233, 234, 235, 236, 268, 290, 321, 324, 349, 381, 382, 497, 529, 530, 532

4ap9A Crystal structure of phosphoserine phosphatase from t.Onnurineus in complex with ndsb-201 (see paper)
28% identity, 61% coverage: 80:260/295 of query aligns to 9:175/200 of 4ap9A

query
sites
4ap9A

K

K

R

K

F

V

L

A

I

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A

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D
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D

M
x
I

D
x
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F

E

E

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D

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E

E

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A

A

A
x
R

E
x
I

V
x
T

G

G

L

-

K

K

E

R

K

E

V

I

A

E

I

L

T

L

A

E

R

K

A

G

M
x
L

N

S

G

G

E

E

I

V

A

E

F
x
W

E

L

P

D

A

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N

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P

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E

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L

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K

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active site: D15 (= D86), I16 (≠ M87), E17 (≠ D88), G103 (= G176), K141 (≠ I227), D162 (= D247)
binding 3-pyridinium-1-ylpropane-1-sulfonate: R31 (≠ A102), I32 (≠ E103), T33 (≠ V104), L46 (≠ M118), W52 (≠ F124), D140 (= D226), K141 (≠ I227), Y160 (≠ A245), A161 (≠ N246)

Query Sequence

>SMc01494 FitnessBrowser__Smeli:SMc01494
MALVATLIANPSNPVLTPARAEAAAERLDASGLYWLADGIACDIVLRDGAEAGEAEARLR
QAVAGAAIDVAVQDAESRRKRFLIADMDSTMIGQECIDELAAEVGLKEKVAAITARAMNG
EIAFEPALVERVALLQGLPVTVVAEVIATRITLTPGGRELIATMKAKGHYSALVSGGFTV
FTGPIAEKLGFDENRANILVEENGKLTGDVARPILGKQAKVDALIDISERLGITPADVIA
VGDGANDLGMLQLAGTGVALHAKPVVADQAKIRIDHGDLTALLYLQGYRKTDFVT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory