SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SMc01502 FitnessBrowser__Smeli:SMc01502 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

2fdrA Crystal structure of conserved haloacid dehalogenase-like protein of unknown function atu0790 from agrobacterium tumefaciens str. C58
39% identity, 82% coverage: 10:193/224 of query aligns to 4:189/222 of 2fdrA

query
sites
2fdrA

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binding magnesium ion: D8 (= D14), D10 (= D16), D166 (= D170)

4eenA Crystal structure of had family hydrolase dr_1622 from deinococcus radiodurans r1 (target efi-501256) with bound magnesium
35% identity, 72% coverage: 11:171/224 of query aligns to 7:170/229 of 4eenA

query
sites
4eenA

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active site: D10 (= D14), D12 (= D16), V16 (= V20), S18 (= S22), M44 (≠ E48), Y45 (≠ R49), T47 (≠ L51), S109 (= S111), N110 (≠ S112), K144 (= K145), E168 (= E169), D169 (= D170)
binding magnesium ion: D10 (= D14), D12 (= D16), D169 (= D170)

Q8VZ10 Protein SUPPRESSOR OF QUENCHING 1, chloroplastic; EC 3.1.3.- from Arabidopsis thaliana (Mouse-ear cress) (see paper)
31% identity, 93% coverage: 11:218/224 of query aligns to 77:284/1055 of Q8VZ10

query
sites
Q8VZ10

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D80 (= D14) mutation to N: Complete rescue in complementation test of the nonphotochemical quenching (NPQ) phenotype observed in disrupted plants.

Sites not aligning to the query:

431:434 CINC→SINS: No rescue in complementation test of the nonphotochemical quenching (NPQ) phenotype observed in disrupted plants.
859 E→K: In soq1-2; high light intensity-dependent and irreversible nonphotochemical quenching (NPQ) due to a decrease in chlorophyll excited-state lifetime.

1te2A Putative phosphatase ynic from escherichia coli k12
31% identity, 83% coverage: 12:197/224 of query aligns to 7:195/218 of 1te2A

query
sites
1te2A

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active site: D9 (= D14), D11 (= D16), S17 (= S22), D44 (≠ R49), S111 (= S111), A112 (≠ S112), K144 (= K145), E168 (= E169), D169 (= D170)
binding calcium ion: D9 (= D14), D11 (= D16), D169 (= D170)
binding 2-phosphoglycolic acid: D9 (= D14), M10 (≠ C15), D11 (= D16), G47 (= G52), S111 (= S111), A112 (≠ S112), K144 (= K145)

P77247 Hexitol phosphatase B; 2-deoxyglucose-6-phosphate phosphatase; Mannitol-1-phosphatase; Sorbitol-6-phosphatase; Sugar-phosphatase; EC 3.1.3.68; EC 3.1.3.22; EC 3.1.3.50; EC 3.1.3.23 from Escherichia coli (strain K12) (see paper)
31% identity, 83% coverage: 12:197/224 of query aligns to 11:199/222 of P77247

query
sites
P77247

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D13 (= D14) binding ; mutation to A: Loss of phosphatase activity.
D15 (= D16) binding
D173 (= D170) binding

4ygrA Crystal structure of had phosphatase from thermococcus onnurineus (see paper)
28% identity, 88% coverage: 11:206/224 of query aligns to 4:206/215 of 4ygrA

query
sites
4ygrA

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L

L

active site: D7 (= D14), D9 (= D16), E15 (≠ S22), K38 (≠ A43), T109 (≠ S112), S110 (= S113), G111 (≠ Q114), K141 (= K145), G165 (≠ E169), D166 (= D170), L168 (vs. gap), Q170 (≠ A173), D171 (≠ G174)
binding magnesium ion: D7 (= D14), D9 (= D16), D166 (= D170)
binding 2-[n-cyclohexylamino]ethane sulfonic acid: D9 (= D16), L50 (= L55), R53 (≠ M58), W58 (≠ H63), W58 (≠ H63), W61 (≠ H66)

3dv9A Putative beta-phosphoglucomutase from bacteroides vulgatus.
25% identity, 92% coverage: 9:215/224 of query aligns to 24:227/243 of 3dv9A

query
sites
3dv9A

R

K

L

A

V

V

I

L

F

F

D
|
D

C
x
M

D
|
D

G

G

V

V

L

L

V

F

D

D

S
|
S

E

M

P

P

I

N

S

H

L

A

G

E

V

S

L

W

V

H

D

K

A

I

L

M

A

K

A

R

A

F

G

G

V

F

S

G

M

L

T

S

A

R

E

E

A

A

S

Y

E

M

R

H

F
x
E

L

G

G

R

R

T

S

G

L

A

K

S

T

T

M

I

S

N

T

I

I

V

L

S

H

R

E

R

E

E

H

R

G

G

L

H

-

D

A

A

T

T

D

E

E

E

V

-

F

-

L

-

E

-

G

-

M

-

R

-

T

-

R

E

L

I

Y

K

A

A

R

I

F

Y

R

Q

E

A

E

K

L

T

R

E

P

E

I

F

A

N

R

K

I

C

R

P

E

K

A

A

V

E

E

R

G

M

L

P

G

G

T

A

A

L

H

E

C

V

V

L

A

T

S

K

S

I

S

K

Q

S

P

E

E

G

R

L

I

T

R

P

L

M

S

V

L

V

T
|
T

V
x
G

T

S

G

G

L

L

I

L

D

D

L

R

F

L

E

N

-

H

-

N

-

F

P

P

N

G

I

I

F

F

S

Q

A

A

S

N

M

L

-

M

-

V

-

T

-

A

-

F

-

D

V

V

A

K

R

Y

G

G

K
|
K

P

P

A

N

P

P

D

E

L

P

F

Y

L

L

H

M

A

A

S

L

A

K

E

K

M

G

G

G

Y

F

H

K

P

P

S

N

D

E

C

A

I

L

V

V

I

I

E
|
E

D
x
N

S

A

P

P

A

L

G

G

I

V

E

Q

A

A

A

G

K

V

S

A

A

A

G

G

M

I

R

F

V

T

F

I

A

A

F

V

A

N

G

T

G

G

S

P

H

-

A

-

R

-

N

-

D

-

R

L

H

H

R

D

R

N

T

V

L

L

A

L

S

N

L

E

E

G

P

A

D

N

V

L

L

L

F

F

D

H

D

S

M

M

active site: D29 (= D14), M30 (≠ C15), D31 (= D16), S37 (= S22), E65 (≠ F50), T131 (= T123), G132 (≠ V124), K162 (= K145), E186 (= E169), N187 (≠ D170)
binding magnesium ion: D29 (= D14), D31 (= D16), E65 (≠ F50), N187 (≠ D170)

3s6jE The crystal structure of a hydrolase from pseudomonas syringae
30% identity, 75% coverage: 12:179/224 of query aligns to 6:177/220 of 3s6jE

query
sites
3s6jE

I

I

F

F

D
|
D

C

L

D
|
D

G

G

V

T

L

L

V

T

D

D

S
|
S

E

V

P

Y

I

Q

S

N

L

V

G

A

V

A

L

W

V

K

D

E

A

A

L

L

A

D

A

A

A

E

G

N

V

I

S

P

M

L

T

-

A

A

E

M

E

W

A

R

S

I

E

H

R

R

F

K

L
x
I

G

G

R

M

S

S

L

G

K

G

T

L

M

M

S

L

T

K

I

S

L

L

H

S

E

R

E

E

H

T

G

G

L

M

A

S

-

I

T

T

D

D

E

E

V

Q

F

A

-

E

-

R

L

L

E

S

G

E

M

K

R

H

T

A

R

Q

L

A

Y

Y

A

E

R

R

F

L

R

Q

E

H

E

Q

L

I

R

I

P

A

I

L

A

P

R

G

I

A

R

V

E

E

A

L

V

L

E

E

G

T

L

L

G

D

T

K

A

E

H

N

-

L

-

K

-

W

C

C

V

I

A

A

S
x
T

S
|
S

S

G

Q

G

P

I

E

D

R

T

I

A

R

T

L

I

S

N

L

L

T

K

V

A

T

L

G

K

L

L

I

-

D

D

L

I

F

N

E

K

P

I

N

N

I

I

F

V

S

T

A

R

S

D

M

D

V

V

A

S

R

Y

G

G

K
|
K

P

P

A

D

P

P

D

D

L

L

F

F

L

L

H

A

A

A

S

A

A

K

E

K

M

I

G

G

Y

A

H

P

P

I

S

D

D

E

C

C

I

L

V

V

I

I

E
x
G

D
|
D

S

A

P

I

A

W

G

D

I

M

E

L

A

A

K

R

active site: D8 (= D14), D10 (= D16), S16 (= S22), I44 (≠ L51), T110 (≠ S111), S111 (= S112), K143 (= K145), G167 (≠ E169), D168 (= D170)
binding calcium ion: D8 (= D14), D10 (= D16), D168 (= D170)

4g9bA Crystal structure of beta-phosphoglucomutase homolog from escherichia coli, target efi-501172, with bound mg, open lid
29% identity, 81% coverage: 11:191/224 of query aligns to 7:194/227 of 4g9bA

query
sites
4g9bA

V

V

I

I

F

F

D
|
D

C
x
L

D
|
D

G

G

V

V

L

I

V

T

D

D

S
x
T

E

A

P

H

I

L

S

H

L

F

G

Q

V

A

L

W

V

Q

D

Q

A

I

L

A

A

A

A

E

A

I

G

G

V

I

S

S

M

I

T

D

A

A

E

Q

-

F

E

N

A

E

S

S

E

L

R
x
K

F

G

L

I

G

S

R

R

S

D

L

-

K

E

T

S

M

L

S

R

T

R

I

I

L

L

H

-

E

-

E

Q

H

H

G

G

L

-

A

G

T

K

D

E

E

G

V

D

F

F

L

N

E

S

G

Q

M

E

R

R

T

A

R

Q

L

L

Y

-

A

-

R

A

F

Y

R

R

E

K

E

N

L

L

R

L

P

Y

I

V

A

H

R

S

I

L

R

R

E

E

A

L

V

T

E

V

G

N

-

A

-

V

L

L

G

P

T

G

A

I

H

R

C

S

V

L

A

L

S

A

S

D

S

L

Q

R

P

A

E

Q

R

Q

I

I

R

S

L

V

S

G

L

L

-

A

-
x
S

-
x
V

-

S

-

L

-

N

-

A

-

P

T

T

V

I

T

L

G

A

L

A

I

L

D

E

L

L

F

R

E

E

P

F

N

F

I

T

F

F

-

C

-

A

S

D

A

A

S

S

M

Q

V

L

A

K

R

N

G

S

K
|
K

P

P

A

D

P

P

D

E

L

I

F

F

L

L

H

A

A

A

S

C

A

A

E

G

M

L

G

G

Y

V

H

P

P

P

S

Q

D

A

C

C

I

I

V

G

I

I

E
|
E

D
|
D

S

A

P

Q

A

A

G

G

I

I

E

D

A

A

A

I

K

N

S

A

A

S

G

G

M

M

R

R

V

S

F

V

A

G

F

I

A

G

G

A

G

G

active site: D10 (= D14), L11 (≠ C15), D12 (= D16), T18 (≠ S22), K46 (≠ R49), S117 (vs. gap), V118 (vs. gap), K148 (= K145), E172 (= E169), D173 (= D170)
binding magnesium ion: D10 (= D14), D12 (= D16), D173 (= D170)

6w04A Crystal structure of had hydrolase, family ia, variant 3 from entamoeba histolytica hm-1:imss
35% identity, 38% coverage: 98:182/224 of query aligns to 96:181/223 of 6w04A

query
sites
6w04A

E

E

A

A

V

L

E

K

G

K

L

C

G

N

T

I

A

P

H

F

C

T

V

I

A

A

S
x
T

S
|
S

S

S

Q

D

P

W

E

G

R

N

I

V

R

Q

L

V

S

F

L

I

T

Q

V

K

T

Y

G

H

L

L

I

E

D

E

L

W

F

F

E

D

P

I

N

D

-

K

I

I

F

I

S

F

A

N

S

D

M

F

V

T

A

F

R

K

G

G

K
|
K

P

P

A

A

P

P

D

D

L

I

F

Y

L

L

H

K

A

A

S

S

A

K

E

K

M

L

G

G

Y

V

H

S

P

I

S

S

D

H

C

C

I

I

V

V

I

F

E
|
E

D
|
D

S

T

P

I

A

S

G

G

I

I

E

H

A

S

A

A

K

L

S

S

A

A

G

G

active site: T109 (≠ S111), S110 (= S112), K144 (= K145), E168 (= E169), D169 (= D170)
binding magnesium ion: D169 (= D170)

Sites not aligning to the query:

active site: 7, 8, 9, 15, 44
binding magnesium ion: 7, 9

5olwA 5-fluorotryptophan labeled beta-phosphoglucomutase in an open conformation (see paper)
27% identity, 78% coverage: 9:182/224 of query aligns to 3:182/224 of 5olwA

query
sites
5olwA

R

K

L

A

V

V

I

L

F

F

D
|
D

C
x
L

D
|
D

G

G

V

V

L

I

V

T

D

D

S
x
T

E

A

P

E

I

Y

S

H

L

F

G

R

V

A

L

W

V

K

D

A

A

L

L

A

A

E

A

I

G

G

V

I

S

N

M

G

T

V

A

D

E

R

E

Q

A

F

S

N

E

E

R

Q

F

L

L
x
K

G

G

R

V

S

S

L

R

K

E

-

D

T

S

M

L

S

Q

T

K

I

I

L

L

H

D

E

L

E

A

H

D

G

K

L

K

A

V

T

S

D

A

E

E

V

E

F

F

L

K

E

E

-

L

-

A

G

K

M

R

R

K

T

N

R

D

L

N

Y

Y

A

V

R

K

F

M

R

I

E

Q

E

D

L

V

R

S

P
|
P

-

A

-

D

-
x
V

-

Y

-

P

-

G

I

I

A

L

R

Q

I

L

R

L

E

K

A

D

V

L

E

R

G

S

L

N

G

K

T

I

A

K

H

I

C

A

V

L

A

A

S
|
S

S
x
A

S

S

Q

K

P

N

E

G

R

P

I

F

R

L

L

L

S
x
E

-

R

L

M

T
x
N

V

L

T

T

G

G

L

Y

I

F

D

D

L

A

F

-

E

-

P

-

N

-

I

I

F

A

S

D

A

P

S

A

M

E

V

V

A

A

R

A

G

S

K
|
K

P

P

A

A

P

P

D

D

L

I

F

F

L

I

H

A

A

A

S

A

A

H

E

A

M

V

G

G

Y

V

H

A

P

P

S

S

D

E

C

S

I

I

V

G

I

L

E
|
E

D
|
D

S
|
S

P

Q

A

A

G

G

I

I

E

Q

A

A

A

I

K

K

S

D

A

S

G

G

active site: D8 (= D14), L9 (≠ C15), D10 (= D16), T16 (≠ S22), K45 (≠ L51), S114 (= S111), A115 (≠ S112), K145 (= K145), E169 (= E169), D170 (= D170)
binding calcium ion: D8 (= D14), D10 (= D16), P89 (= P92), V92 (vs. gap), E124 (≠ S121), N127 (≠ T123), E169 (= E169), D170 (= D170), S171 (= S171)

P71447 Beta-phosphoglucomutase; Beta-PGM; EC 5.4.2.6 from Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) (see 3 papers)
27% identity, 78% coverage: 9:182/224 of query aligns to 3:182/221 of P71447

query
sites
P71447

R

K

L

A

V

V

I

L

F

F

D
|
D

C

L

D
|
D

G

G

V

V

L

I

V

T

D

D

S
x
T

E

A

P

E

I

Y

S
x
H

L

F

G

R

V

A

L

W

V

K

D

A

A

L

L

A

A

E

A

I

G

G

V

I

S

N

M

G

T

V

A

D

E

R

E

Q

A

F

S

N

E

E

R

Q

F

L

L
x
K

G
|
G

R

V

S

S

L
x
R

K

E

-

D

T
x
S

M

L

S

Q

T

K

I

I

L

L

H

D

E

L

E

A

H

D

G

K

L

K

A

V

T

S

D

A

E

E

V

E

F

F

L

K

E

E

-

L

-

A

G

K

M

R

R
x
K

T

N

R

D

L

N

Y

Y

A

V

R

K

F

M

R

I

E

Q

E

D

L

V

R

S

P

P

-

A

-

D

-

V

-

Y

-

P

-

G

I

I

A

L

R

Q

I

L

R

L

E

K

A

D

V

L

E

R

G

S

L

N

G

K

T

I

A

K

H

I

C

A

V

L

A

A

S

S

S

A

S

S

Q

K

P

N

E

G

R

P

I

F

R

L

L

L

-

E

S

K

L

M

T

N

V

L

T

T

G

G

L

Y

I

F

D

D

L

A

F

-

E

-

P

-

N

-

I

I

F

A

S

D

A

P

S

A

M

E

V

V

A

A

R

A

G

S

K

K

P

P

A

A

P

P

D

D

L

I

F

F

L

I

H

A

A

A

S

A

A

H

E

A

M

V

G

G

Y

V

H

A

P

P

S

S

D

E

C

S

I

I

V

G

I

L

E

E

D
|
D

S

S

P

Q

A

A

G

G

I

I

E

Q

A

A

A

I

K

K

S

D

A

S

G

G

D8 (= D14) modified: 4-aspartylphosphate; mutation to A: Inactive.; mutation to E: Inactive.
D10 (= D16) mutation to A: Inactive.; mutation to E: Inactive.; mutation to N: Inactive.; mutation to S: Inactive.
T16 (≠ S22) mutation to P: 500-fold reduction in the rate constant for Asp-8 phosphorylation by beta-G1,6bisP. 6,700-fold reduction in the apparent rate constant for cycling of the phosphorylated enzyme to convert beta-G1P to G6P. 13-fold increase in the estimated rate constant for phosphoryl transfer from the phospho-Asp8 to water.
H20 (≠ S26) mutation to A: Impairs Asp-8 phosphorylation by beta-G1,6bisP and phosphoryl transfer from the phospho-Asp8 to the substrate beta-G1P.; mutation to N: 300-fold reduction in the conversion of beta-G1P to G6P in the presence of beta-G1,6bisP.; mutation to Q: 8-fold reduction in the conversion of beta-G1P to G6P in the presence of beta-G1,6bisP.
K45 (≠ L51) mutation to A: 20'000-fold decrease in kcat/KM.
G46 (= G52) mutation to A: 1'000'000-fold decrease in kcat/KM.; mutation to P: 100'000-fold decrease in kcat/KM.; mutation to V: 10'000-fold decrease in kcat/KM.
R49 (≠ L55) mutation to K: 1'000'000-fold decrease in kcat/KM.
S52 (≠ T57) mutation to A: Wild-type activity.
K76 (≠ R79) mutation to A: 100-fold reduction in the conversion of beta-G1P to G6P in the presence of beta-G1,6bisP.
D170 (= D170) mutation to A: Impaired, but active with an increase in the affinity for G1P.

6h91A Phosphorylated beta-phosphoglucomutase from lactococcus lactis in an open conformer to 2.4 a
27% identity, 78% coverage: 9:182/224 of query aligns to 3:182/218 of 6h91A

query
sites
6h91A

R

K

L

A

V

V

I

L

F

F

D
|
D

C

L

D
|
D

G

G

V

V

L

I

V

T

D

D

S

T

E

A

P

E

I

Y

S

H

L

F

G

R

V

A

L

W

V

K

D

A

A

L

L

A

A

E

A

I

G

G

V

I

S

N

M

G

T

V

A

D

E

R

E

Q

A

F

S

N

E

E

R

Q

F

L

L

K

G

G

R

V

S

S

L
x
R

K

E

-

D

T

S

M

L

S

Q

T

K

I

I

L

L

H

D

E

L

E

A

H

D

G

K

L

K

A

V

T

S

D

A

E

E

V

E

F

F

L

K

E

E

-

L

-

A

G

K

M

R

R

K

T

N

R

D

L

N

Y

Y

A

V

R

K

F

M

R

I

E

Q

E

D

L

V

R

S

P

P

-

A

-

D

-

V

-

Y

-

P

-

G

I

I

A

L

R

Q

I

L

R

L

E

K

A

D

V

L

E

R

G

S

L

N

G

K

T

I

A

K

H

I

C

A

V

L

A

A

S

S

S

A

S

S

Q
x
K

P

N

E

G

R

P

I

F

R

L

L

L

S

E

-

R

L

M

T

N

V

L

T

T

G

G

L

Y

I

F

D

D

L

A

F

-

E

-

P

-

N

-

I

I

F

A

S

D

A

P

S

A

M

E

V

V

A

A

R

A

G

S

K

K

P

P

A

A

P

P

D

D

L

I

F

F

L

I

H

A

A

A

S

A

A

H

E

A

M

V

G

G

Y

V

H

A

P

P

S

S

D

E

C

S

I

I

V

G

I

L

E

E

D
|
D

S

S

P

Q

A

A

G

G

I

I

E

Q

A

A

A

I

K

K

S

D

A

S

G

G

binding magnesium ion: D8 (= D14), D10 (= D16), D170 (= D170)
binding acetylphosphate: R49 (≠ L55), K117 (≠ Q114)

4c4rA Structure of beta-phosphoglucomutase in complex with a phosphonate analogue of beta-glucose-1-phosphate and magnesium trifluoride (see paper)
27% identity, 78% coverage: 9:182/224 of query aligns to 3:182/218 of 4c4rA

query
sites
4c4rA

R

K

L

A

V

V

I

L

F

F

D
|
D

C
x
L

D
|
D

G

G

V

V

L

I

V

T

D

D

S
x
T

E

A

P

E

I

Y

S
x
H

L

F

G

R

V

A

L
x
W

V

K

D

A

A

L

L

A

A

E

A

I

G

G

V

I

S

N

M

G

T

V

A

D

E

R

E

Q

A

F

S

N

E

E

R

Q

F
x
L

L
x
K

G
|
G

R
x
V

S

S

L
x
R

K

E

-

D

T
x
S

M

L

S

Q

T

K

I

I

L

L

H

D

E

L

E

A

H

D

G

K

L

K

A

V

T

S

D

A

E

E

V

E

F

F

L

K

E

E

-

L

-

A

G

K

M

R

R

K

T

N

R

D

L

N

Y

Y

A

V

R

K

F

M

R

I

E

Q

E

D

L

V

R

S

P

P

-

A

-

D

-

V

-

Y

-

P

-

G

I

I

A

L

R

Q

I

L

R

L

E

K

A

D

V

L

E

R

G

S

L

N

G

K

T

I

A

K

H

I

C

A

V

L

A

A

S
|
S

S
x
A

S
|
S

Q
x
K

P

N

E

G

R

P

I

F

R

L

L

L

S

E

-

R

L

M

T

N

V

L

T

T

G

G

L

Y

I

F

D

D

L

A

F

-

E

-

P

-

N

-

I

I

F

A

S

D

A

P

S

A

M

E

V

V

A

A

R

A

G

S

K
|
K

P

P

A

A

P

P

D

D

L

I

F

F

L

I

H

A

A

A

S

A

A

H

E

A

M

V

G

G

Y

V

H

A

P

P

S

S

D

E

C

S

I

I

V

G

I

L

E
|
E

D
|
D

S

S

P

Q

A

A

G

G

I

I

E

Q

A

A

A

I

K

K

S

D

A

S

G

G

active site: D8 (= D14), L9 (≠ C15), D10 (= D16), T16 (≠ S22), K45 (≠ L51), S114 (= S111), A115 (≠ S112), K145 (= K145), E169 (= E169), D170 (= D170)
binding magnesium ion: D8 (= D14), D10 (= D16), D170 (= D170)
binding trifluoromagnesate: D8 (= D14), L9 (≠ C15), D10 (= D16), S114 (= S111), A115 (≠ S112), K145 (= K145)
binding (1R)-1,5-anhydro-1-(phosphonomethyl)-D-glucitol: D10 (= D16), H20 (≠ S26), W24 (≠ L30), L44 (≠ F50), G46 (= G52), V47 (≠ R53), R49 (≠ L55), S52 (≠ T57), S116 (= S113), K117 (≠ Q114)

3zi4A The structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate and scandium tetrafluoride
27% identity, 78% coverage: 9:182/224 of query aligns to 3:182/218 of 3zi4A

query
sites
3zi4A

R

K

L

A

V

V

I

L

F

F

D
|
D

C
x
L

D
|
D

G

G

V

V

L

I

V

T

D

D

S
x
T

E

A

P

E

I

Y

S
x
H

L

F

G

R

V

A

L

W

V

K

D

A

A

L

L

A

A

E

A

I

G

G

V

I

S

N

M

G

T

V

A

D

E

R

E

Q

A

F

S

N

E

E

R

Q

F

L

L
x
K

G
|
G

R
x
V

S

S

L
x
R

K

E

-

D

T

S

M

L

S

Q

T

K

I

I

L

L

H

D

E

L

E

A

H

D

G

K

L

K

A

V

T

S

D

A

E

E

V

E

F

F

L

K

E

E

-

L

-

A

G

K

M

R

R

K

T

N

R

D

L

N

Y

Y

A

V

R

K

F

M

R

I

E

Q

E

D

L

V

R

S

P

P

-

A

-

D

-

V

-

Y

-

P

-

G

I

I

A

L

R

Q

I

L

R

L

E

K

A

D

V

L

E

R

G

S

L

N

G

K

T

I

A

K

H

I

C

A

V

L

A

A

S
|
S

S
x
A

S
|
S

Q
x
K

P

N

E

G

R

P

I

F

R

L

L

L

S

E

-

R

L

M

T

N

V

L

T

T

G

G

L

Y

I

F

D

D

L

A

F

-

E

-

P

-

N

-

I

I

F

A

S

D

A

P

S

A

M

E

V

V

A

A

R

A

G

S

K
|
K

P

P

A

A

P

P

D

D

L

I

F

F

L

I

H

A

A

A

S

A

A

H

E

A

M

V

G

G

Y

V

H

A

P

P

S

S

D

E

C

S

I

I

V

G

I

L

E
|
E

D
|
D

S

S

P

Q

A

A

G

G

I

I

E

Q

A

A

A

I

K

K

S

D

A

S

G

G

active site: D8 (= D14), L9 (≠ C15), D10 (= D16), T16 (≠ S22), K45 (≠ L51), S114 (= S111), A115 (≠ S112), K145 (= K145), E169 (= E169), D170 (= D170)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D16), H20 (≠ S26), G46 (= G52), V47 (≠ R53), R49 (≠ L55), S116 (= S113), K117 (≠ Q114)
binding magnesium ion: D8 (= D14), D10 (= D16), D170 (= D170)
binding Scandium Tetrafluoride: D8 (= D14), L9 (≠ C15), D10 (= D16), S114 (= S111), A115 (≠ S112), K145 (= K145)

2wf8A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate, glucose-1-phosphate and beryllium trifluoride (see paper)
27% identity, 78% coverage: 9:182/224 of query aligns to 3:182/218 of 2wf8A

query
sites
2wf8A

R

K

L

A

V

V

I

L

F

F

D
|
D

C
x
L

D
|
D

G

G

V

V

L

I

V

T

D

D

S
x
T

E

A

P

E

I

Y

S
x
H

L

F

G

R

V

A

L
x
W

V

K

D

A

A

L

L

A

A

E

A

I

G

G

V

I

S

N

M

G

T

V

A

D

E

R

E

Q

A

F

S

N

E

E

R

Q

F
x
L

L
x
K

G
|
G

R
x
V

S

S

L
x
R

K

E

-

D

T
x
S

M

L

S

Q

T

K

I

I

L

L

H

D

E

L

E

A

H

D

G

K

L

K

A

V

T

S

D

A

E

E

V

E

F

F

L

K

E

E

-

L

-

A

G

K

M

R

R

K

T

N

R

D

L

N

Y

Y

A

V

R

K

F

M

R

I

E

Q

E

D

L

V

R

S

P

P

-

A

-

D

-

V

-

Y

-

P

-

G

I

I

A

L

R

Q

I

L

R

L

E

K

A

D

V

L

E

R

G

S

L

N

G

K

T

I

A

K

H

I

C

A

V

L

A

A

S
|
S

S
x
A

S
|
S

Q
x
K

P

N

E

G

R

P

I

F

R

L

L

L

S

E

-

R

L

M

T

N

V

L

T

T

G

G

L

Y

I

F

D

D

L

A

F

-

E

-

P

-

N

-

I

I

F

A

S

D

A

P

S

A

M

E

V

V

A

A

R

A

G

S

K
|
K

P

P

A

A

P

P

D

D

L

I

F

F

L

I

H

A

A

A

S

A

A

H

E

A

M

V

G

G

Y

V

H

A

P

P

S

S

D

E

C

S

I

I

V

G

I

L

E
|
E

D
|
D

S

S

P

Q

A

A

G

G

I

I

E

Q

A

A

A

I

K

K

S

D

A

S

G

G

active site: D8 (= D14), L9 (≠ C15), D10 (= D16), T16 (≠ S22), K45 (≠ L51), S114 (= S111), A115 (≠ S112), K145 (= K145), E169 (= E169), D170 (= D170)
binding beryllium trifluoride ion: D8 (= D14), L9 (≠ C15), D10 (= D16), S114 (= S111), A115 (≠ S112), K145 (= K145)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D16), H20 (≠ S26), G46 (= G52), V47 (≠ R53), R49 (≠ L55), A115 (≠ S112), S116 (= S113), K117 (≠ Q114)
binding 1-O-phosphono-alpha-D-glucopyranose: D10 (= D16), H20 (≠ S26), W24 (≠ L30), L44 (≠ F50), G46 (= G52), V47 (≠ R53), R49 (≠ L55), S52 (≠ T57), A115 (≠ S112), S116 (= S113), K117 (≠ Q114)
binding magnesium ion: D8 (= D14), D10 (= D16), D170 (= D170)

2wf7A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphonate and aluminium tetrafluoride (see paper)
27% identity, 78% coverage: 9:182/224 of query aligns to 3:182/218 of 2wf7A

query
sites
2wf7A

R

K

L

A

V

V

I

L

F

F

D
|
D

C
x
L

D
|
D

G

G

V

V

L

I

V

T

D

D

S
x
T

E

A

P

E

I

Y

S

H

L

F

G

R

V

A

L

W

V

K

D

A

A

L

L

A

A

E

A

I

G

G

V

I

S

N

M

G

T

V

A

D

E

R

E

Q

A

F

S

N

E

E

R

Q

F

L

L
x
K

G
|
G

R
x
V

S

S

L
x
R

K

E

-

D

T

S

M

L

S

Q

T

K

I

I

L

L

H

D

E

L

E

A

H

D

G

K

L

K

A

V

T

S

D

A

E

E

V

E

F

F

L

K

E

E

-

L

-

A

G

K

M

R

R

K

T

N

R

D

L

N

Y

Y

A

V

R

K

F

M

R

I

E

Q

E

D

L

V

R

S

P

P

-

A

-

D

-

V

-

Y

-

P

-

G

I

I

A

L

R

Q

I

L

R

L

E

K

A

D

V

L

E

R

G

S

L

N

G

K

T

I

A

K

H

I

C

A

V

L

A

A

S
|
S

S
x
A

S
|
S

Q
x
K

P
x
N

E

G

R

P

I

F

R

L

L

L

S

E

-

R

L

M

T

N

V

L

T

T

G

G

L

Y

I

F

D

D

L

A

F

-

E

-

P

-

N

-

I

I

F

A

S

D

A

P

S

A

M

E

V

V

A

A

R

A

G

S

K
|
K

P

P

A

A

P

P

D

D

L

I

F

F

L

I

H

A

A

A

S

A

A

H

E

A

M

V

G

G

Y

V

H

A

P

P

S

S

D

E

C

S

I

I

V

G

I

L

E
|
E

D
|
D

S

S

P

Q

A

A

G

G

I

I

E

Q

A

A

A

I

K

K

S

D

A

S

G

G

active site: D8 (= D14), L9 (≠ C15), D10 (= D16), T16 (≠ S22), K45 (≠ L51), S114 (= S111), A115 (≠ S112), K145 (= K145), E169 (= E169), D170 (= D170)
binding tetrafluoroaluminate ion: D8 (= D14), L9 (≠ C15), D10 (= D16), S114 (= S111), K145 (= K145)
binding 6,7-dideoxy-7-phosphono-beta-D-gluco-heptopyranose: D10 (= D16), G46 (= G52), V47 (≠ R53), R49 (≠ L55), S116 (= S113), K117 (≠ Q114), N118 (≠ P115)
binding magnesium ion: D8 (= D14), D10 (= D16), D170 (= D170)

2wf6A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate and aluminium tetrafluoride (see paper)
27% identity, 78% coverage: 9:182/224 of query aligns to 3:182/218 of 2wf6A

query
sites
2wf6A

R

K

L

A

V

V

I

L

F

F

D
|
D

C
x
L

D
|
D

G

G

V

V

L

I

V

T

D

D

S
x
T

E

A

P

E

I

Y

S

H

L

F

G

R

V

A

L

W

V

K

D

A

A

L

L

A

A

E

A

I

G

G

V

I

S

N

M

G

T

V

A

D

E

R

E

Q

A

F

S

N

E

E

R

Q

F

L

L
x
K

G
|
G

R
x
V

S

S

L
x
R

K

E

-

D

T

S

M

L

S

Q

T

K

I

I

L

L

H

D

E

L

E

A

H

D

G

K

L

K

A

V

T

S

D

A

E

E

V

E

F

F

L

K

E

E

-

L

-

A

G

K

M

R

R

K

T

N

R

D

L

N

Y

Y

A

V

R

K

F

M

R

I

E

Q

E

D

L

V

R

S

P

P

-

A

-

D

-

V

-

Y

-

P

-

G

I

I

A

L

R

Q

I

L

R

L

E

K

A

D

V

L

E

R

G

S

L

N

G

K

T

I

A

K

H

I

C

A

V

L

A

A

S
|
S

S
x
A

S
|
S

Q
x
K

P

N

E

G

R

P

I

F

R

L

L

L

S

E

-

R

L

M

T

N

V

L

T

T

G

G

L

Y

I

F

D

D

L

A

F

-

E

-

P

-

N

-

I

I

F

A

S

D

A

P

S

A

M

E

V

V

A

A

R

A

G

S

K
|
K

P

P

A

A

P

P

D

D

L

I

F

F

L

I

H

A

A

A

S

A

A

H

E

A

M

V

G

G

Y

V

H

A

P

P

S

S

D

E

C

S

I

I

V

G

I

L

E
|
E

D
|
D

S

S

P

Q

A

A

G

G

I

I

E

Q

A

A

A

I

K

K

S

D

A

S

G

G

active site: D8 (= D14), L9 (≠ C15), D10 (= D16), T16 (≠ S22), K45 (≠ L51), S114 (= S111), A115 (≠ S112), K145 (= K145), E169 (= E169), D170 (= D170)
binding tetrafluoroaluminate ion: D8 (= D14), L9 (≠ C15), D10 (= D16), S114 (= S111), K145 (= K145)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D16), G46 (= G52), V47 (≠ R53), R49 (≠ L55), S116 (= S113), K117 (≠ Q114)
binding magnesium ion: D8 (= D14), D10 (= D16), D170 (= D170)

2wf5A Structure of beta-phosphoglucomutase inhibited with glucose-6- phosphate and trifluoromagnesate (see paper)
27% identity, 78% coverage: 9:182/224 of query aligns to 3:182/218 of 2wf5A

query
sites
2wf5A

R

K

L

A

V

V

I

L

F

F

D
|
D

C
x
L

D
|
D

G

G

V

V

L

I

V

T

D

D

S
x
T

E

A

P

E

I

Y

S
x
H

L

F

G

R

V

A

L

W

V

K

D

A

A

L

L

A

A

E

A

I

G

G

V

I

S

N

M

G

T

V

A

D

E

R

E

Q

A

F

S

N

E

E

R

Q

F

L

L
x
K

G
|
G

R
x
V

S

S

L
x
R

K

E

-

D

T

S

M

L

S

Q

T

K

I

I

L

L

H

D

E

L

E

A

H

D

G

K

L

K

A

V

T

S

D

A

E

E

V

E

F

F

L

K

E

E

-

L

-

A

G

K

M

R

R

K

T

N

R

D

L

N

Y

Y

A

V

R

K

F

M

R

I

E

Q

E

D

L

V

R

S

P

P

-

A

-

D

-

V

-

Y

-

P

-

G

I

I

A

L

R

Q

I

L

R

L

E

K

A

D

V

L

E

R

G

S

L

N

G

K

T

I

A

K

H

I

C

A

V

L

A

A

S
|
S

S
x
A

S
|
S

Q

K

P

N

E

G

R

P

I

F

R

L

L

L

S

E

-

R

L

M

T

N

V

L

T

T

G

G

L

Y

I

F

D

D

L

A

F

-

E

-

P

-

N

-

I

I

F

A

S

D

A

P

S

A

M

E

V

V

A

A

R

A

G

S

K
|
K

P

P

A

A

P

P

D

D

L

I

F

F

L

I

H

A

A

A

S

A

A

H

E

A

M

V

G

G

Y

V

H

A

P

P

S

S

D

E

C

S

I

I

V

G

I

L

E
|
E

D
|
D

S

S

P

Q

A

A

G

G

I

I

E

Q

A

A

A

I

K

K

S

D

A

S

G

G

active site: D8 (= D14), L9 (≠ C15), D10 (= D16), T16 (≠ S22), K45 (≠ L51), S114 (= S111), A115 (≠ S112), K145 (= K145), E169 (= E169), D170 (= D170)
binding 6-O-phosphono-beta-D-glucopyranose: D10 (= D16), H20 (≠ S26), G46 (= G52), V47 (≠ R53), R49 (≠ L55), A115 (≠ S112), S116 (= S113)
binding magnesium ion: D8 (= D14), D10 (= D16), D170 (= D170)
binding trifluoromagnesate: D8 (= D14), L9 (≠ C15), D10 (= D16), S114 (= S111), A115 (≠ S112), K145 (= K145)

6qzgA Beta-glucose 1,6-bisphosphonate bound to wild type beta- phosphoglucomutse in an open conformation.
27% identity, 78% coverage: 9:182/224 of query aligns to 3:182/219 of 6qzgA

query
sites
6qzgA

R

K

L

A

V

V

I

L

F

F

D
|
D

C
x
L

D
|
D

G

G

V

V

L

I

V

T

D

D

S

T

E

A

P

E

I

Y

S
x
H

L

F

G

R

V

A

L

W

V

K

D

A

A

L

L

A

A

E

A

I

G

G

V

I

S

N

M

G

T

V

A

D

E

R

E

Q

A

F

S

N

E

E

R

Q

F

L

L

K

G
|
G

R

V

S

S

L

R

K

E

-

D

T

S

M

L

S

Q

T

K

I

I

L

L

H

D

E

L

E

A

H

D

G

K

L

K

A

V

T

S

D

A

E

E

V

E

F

F

L

K

E

E

-

L

-

A

G

K

M

R

R

K

T

N

R

D

L

N

Y

Y

A

V

R

K

F

M

R

I

E

Q

E

D

L

V

R

S

P

P

-

A

-

D

-

V

-

Y

-

P

-

G

I

I

A

L

R

Q

I

L

R

L

E

K

A

D

V

L

E

R

G

S

L

N

G

K

T

I

A

K

H

I

C

A

V

L

A

A

S
|
S

S
x
A

S
|
S

Q
x
K

P

N

E

G

R

P

I

F

R

L

L

L

S

E

-

R

L

M

T

N

V

L

T

T

G

G

L

Y

I

F

D

D

L

A

F

-

E

-

P

-

N

-

I

I

F

A

S

D

A

P

S

A

M

E

V

V

A

A

R

A

G

S

K
|
K

P

P

A

A

P

P

D

D

L

I

F

F

L

I

H

A

A

A

S

A

A

H

E

A

M

V

G

G

Y

V

H

A

P

P

S

S

D

E

C

S

I

I

V

G

I

L

E

E

D
|
D

S

S

P

Q

A

A

G

G

I

I

E

Q

A

A

A

I

K

K

S

D

A

S

G

G

binding 3,7-anhydro-1,2,8-trideoxy-1,8-diphosphono-D-glycero-D-gulo-octitol: D8 (= D14), L9 (≠ C15), D10 (= D16), H20 (≠ S26), G46 (= G52), S114 (= S111), A115 (≠ S112), S116 (= S113), K117 (≠ Q114), K145 (= K145)
binding magnesium ion: D8 (= D14), D10 (= D16), D170 (= D170)

Query Sequence

>SMc01502 FitnessBrowser__Smeli:SMc01502
MTMVGHASRLVIFDCDGVLVDSEPISLGVLVDALAAAGVSMTAEEASERFLGRSLKTMST
ILHEEHGLATDEVFLEGMRTRLYARFREELRPIARIREAVEGLGTAHCVASSSQPERIRL
SLTVTGLIDLFEPNIFSASMVARGKPAPDLFLHASAEMGYHPSDCIVIEDSPAGIEAAKS
AGMRVFAFAGGSHARNDRHRRTLASLEPDVLFDDMGELIQFVRQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory