SitesBLAST
Comparing SMc01534 FitnessBrowser__Smeli:SMc01534 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q9A3Q9 Omega-aminotransferase; Beta-alanine--pyruvate aminotransferase; EC 2.6.1.-; EC 2.6.1.18 from Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus) (see paper)
64% identity, 97% coverage: 10:438/442 of query aligns to 7:435/439 of Q9A3Q9
- V227 (= V230) mutation to G: Decreases activity toward 3-aminobutanoate by 2-fold. Increases activity toward the aromatic beta-amino acid 3-amino-3-phenylpropanoate by 2-fold.
- R260 (= R263) mutation to L: Decreases activity toward 3-aminobutanoate by 30-fold.
- N285 (= N288) mutation to A: Decreases activity toward 3-aminobutanoate by 4-fold. Increases activity toward the aromatic beta-amino acid 3-amino-3-phenylpropanoate by 3-fold.
Q9I700 Beta-alanine--pyruvate aminotransferase; Beta-A--Py AT; Beta-alanine--pyruvate transaminase; Omega-amino acid aminotransferase; Omega-amino acid AT; Omega-amino acid--pyruvate aminotransferase; Omega-APT; EC 2.6.1.18 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
51% identity, 99% coverage: 1:439/442 of query aligns to 1:445/448 of Q9I700
- W61 (= W56) binding
- T327 (= T321) binding
- R414 (= R408) binding
- Q421 (≠ A415) binding
4b9bA The structure of the omega aminotransferase from pseudomonas aeruginosa (see paper)
52% identity, 97% coverage: 11:439/442 of query aligns to 4:433/436 of 4b9bA
- active site: F12 (= F19), Y141 (= Y148), E214 (= E222), D247 (= D255), I250 (= I258), K276 (= K284), Q409 (≠ A415)
- binding calcium ion: D168 (= D176), H169 (= H177)
- binding pyridoxal-5'-phosphate: S107 (= S114), G108 (= G115), S109 (= S116), Y141 (= Y148), H142 (= H149), G143 (= G150), E214 (= E222), D247 (= D255), V249 (= V257), I250 (= I258), K276 (= K284), Y314 (= Y320), T315 (= T321)
4b98A The structure of the omega aminotransferase from pseudomonas aeruginosa (see paper)
52% identity, 97% coverage: 11:439/442 of query aligns to 9:438/441 of 4b98A
- active site: F17 (= F19), Y146 (= Y148), E219 (= E222), D252 (= D255), I255 (= I258), K281 (= K284), Q414 (≠ A415)
- binding pyridoxal-5'-phosphate: M189 (≠ E193), V232 (≠ K235), G233 (= G236), Y234 (= Y237), L235 (= L238), Q236 (= Q239), F270 (= F273), G271 (≠ D274), V272 (= V275)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: L53 (= L55), W54 (= W56), F82 (= F84), S112 (= S114), G113 (= G115), S114 (= S116), Y146 (= Y148), H147 (= H149), G148 (= G150), E219 (= E222), S224 (= S227), D252 (= D255), V254 (= V257), I255 (= I258), K281 (= K284), Y319 (= Y320), T320 (= T321), R407 (= R408), Q414 (≠ A415)
4uhmA Characterization of a novel transaminase from pseudomonas sp. Strain aac (see paper)
50% identity, 97% coverage: 11:439/442 of query aligns to 3:432/435 of 4uhmA
- active site: F11 (= F19), Y140 (= Y148), E213 (= E222), D246 (= D255), I249 (= I258), K275 (= K284), Q408 (≠ A415)
- binding magnesium ion: A91 (≠ V99), D99 (≠ N107)
- binding pyridoxal-5'-phosphate: S106 (= S114), G107 (= G115), S108 (= S116), Y140 (= Y148), H141 (= H149), G142 (= G150), E213 (= E222), S218 (= S227), D246 (= D255), V248 (= V257), I249 (= I258), K275 (= K284)
3a8uX Crystal structure of omega-amino acid:pyruvate aminotransferase
50% identity, 97% coverage: 12:439/442 of query aligns to 9:438/441 of 3a8uX
- active site: Y145 (= Y148), D252 (= D255), K281 (= K284), Q414 (≠ A415)
- binding pyridoxal-5'-phosphate: S111 (= S114), G112 (= G115), S113 (= S116), Y145 (= Y148), H146 (= H149), G147 (= G150), E219 (= E222), D252 (= D255), V254 (= V257), I255 (= I258), K281 (= K284)
7qx3A Aminotransferase TR2 (see paper)
39% identity, 93% coverage: 30:438/442 of query aligns to 2:414/422 of 7qx3A
6gwiB The crystal structure of halomonas elongata amino-transferase (see paper)
37% identity, 96% coverage: 18:441/442 of query aligns to 17:445/450 of 6gwiB
- active site: F18 (= F19), Y149 (= Y148), D255 (= D255), K284 (= K284)
- binding pyridoxal-5'-phosphate: S115 (= S114), G116 (= G115), S117 (= S116), N120 (≠ V119), Y149 (= Y148), H150 (= H149), G151 (= G150), E222 (= E222), D255 (= D255), V257 (= V257), I258 (= I258), K284 (= K284), F317 (≠ Y320), T318 (= T321)
7qx0B Aminotransferase TR2 (see paper)
37% identity, 95% coverage: 18:438/442 of query aligns to 16:435/443 of 7qx0B
- binding pyridoxal-5'-phosphate: S114 (= S114), G115 (= G115), S116 (= S116), Y148 (= Y148), H149 (= H149), G150 (= G150), E221 (= E222), D254 (= D255), V256 (= V257), I257 (= I258), K283 (= K284)
5lhaA Amine transaminase crystal structure from an uncultivated pseudomonas species in the pmp-bound form
39% identity, 96% coverage: 10:434/442 of query aligns to 5:439/447 of 5lhaA
- active site: Y146 (= Y148), D253 (= D255), K282 (= K284), T319 (= T321)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G112 (≠ S114), G113 (= G115), S114 (= S116), Y146 (= Y148), H147 (= H149), G148 (= G150), E220 (= E222), D253 (= D255), V255 (= V257), V256 (≠ I258), K282 (= K284), Y318 (= Y320), T319 (= T321)
5lh9D Amine transaminase crystal structure from an uncultivated pseudomonas species in the plp-bound (internal aldimine) form
39% identity, 96% coverage: 10:434/442 of query aligns to 7:441/449 of 5lh9D