SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SMc01578 FitnessBrowser__Smeli:SMc01578 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q02635 Aspartate/prephenate aminotransferase; AspAT / PAT; Transaminase A; EC 2.6.1.1; EC 2.6.1.79 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
100% identity, 100% coverage: 1:400/400 of query aligns to 1:400/400 of Q02635

query
sites
Q02635

M

M

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A

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F

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L

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A

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D

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A

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L

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S

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K

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P

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I

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Q

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G

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E

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P

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F

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D

T

T

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P

D

D

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I

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K

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K

A

A

A

A

I

I

D

D

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I

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D

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R

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G

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E

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K

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Y

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G

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E

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E

E

A

A

I

I

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A

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K

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F

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K

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E

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N

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D

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Y

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A

A

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T

I

I

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V

G

G

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T

G

G

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G

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K

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Q

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I

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L

F

F

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N

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A

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F

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M

A

A

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T

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L

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P

G

G

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Y

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E

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F

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K12 (= K12) Important for prephenate aminotransferase activity; mutation to G: 7-fold increase in Km for prephenate. Weakly affects Km for oxaloacetate.

6f77A Crystal structure of the prephenate aminotransferase from rhizobium meliloti (see paper)
100% identity, 100% coverage: 2:400/400 of query aligns to 1:399/399 of 6f77A

query
sites
6f77A

A

A

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G

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D

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I

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K

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A

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A

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I

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I

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A

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E

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Y

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A

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A

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Q

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T

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I

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T

G
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G

G
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G

K
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K

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I

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P

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N

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S

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G

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A

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M

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T

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G

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binding pyridoxal-5'-phosphate: G98 (= G99), G99 (= G100), K100 (= K101), W124 (= W125), N170 (= N171), N174 (= N175), D203 (= D204), Y206 (= Y207), S237 (= S238), K238 (= K239), R246 (= R247)

P58350 Aspartate aminotransferase; AAT; AspAT; Putative 2-aminoadipate transaminase; Transaminase A; EC 2.6.1.1; EC 2.6.1.39 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
59% identity, 98% coverage: 5:396/400 of query aligns to 15:406/410 of P58350

query
sites
P58350

A

A

D

S

A

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L

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G

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K

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G

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A

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G

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E

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F

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A

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G

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E

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T

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K

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E

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A

A

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K

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F

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E

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G

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L

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G

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A

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K

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C

K249 (= K239) modified: N6-(pyridoxal phosphate)lysine

6f35A Crystal structure of the aspartate aminotranferase from rhizobium meliloti (see paper)
59% identity, 98% coverage: 5:396/400 of query aligns to 5:396/400 of 6f35A

query
sites
6f35A

A

A

D

S

A

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E

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A

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A

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A

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G

E

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A

V

L

S

D

G

G

I

T

P

P

E

E

L

L

R

K

E

K

A

A

I

I

A

R

K

E

K

K

F

F

K

Q

R

R

E

E

N

N

N

G

L

L

D

A

Y

Y

T

E

A

L

A

D

Q

E

T

I

I

T

V

V

G

A

T

T

G
|
G

G
x
A

K
|
K

Q

Q

I

I

L

L

F

F

N

N

A

A

F

M

M

M

A

A

T

S

L

L

N

D

P

P

G

G

D

D

E

E

V

V

V

I

I

I

P

P

A

T

P

P

Y

Y

W

W

V

T

S

S

Y

Y

P

S

E

D

M

I

V

V

A

H

L

I

C

C

G

E

G

G

T

K

P

P

V

V

F

L

V

I

P

A

T

C

R

D

Q

A

E

S

N

S

N

G

F

F

K

R

L

L

K

T

A

A

E

E

D

K

L

L

D

E

R

A

A

A

I

I

T

T

P

P

K

R

T

T

K

R

W

W

F

V

V

L

F

L

N

N

S

S

P

P

S

S

N

N

P

P

S

S

G

G

A

A

A

A

Y

Y

S

S

H

A

E

A

E

D

L

Y

K

R

A

P

L

L

T

L

D

E

V

V

L

L

M

L

K

R

H

H

P

P

H

H

V

V

W

W

V

L

L

L

T

V

D
|
D

D

D

M
|
M

Y
|
Y

E

E

H

H

L

I

T

V

Y

Y

G

D

D

G

F

F

R

R

F

F

A

V

T

T

P

P

V

A

E

Q

V

L

E

E

P

P

G

G

L

L

Y

K

E

N

R

R

T

T

L

L

T

T

M

V

N

N

G

G

V

V

S
|
S

K
|
K

A

A

Y

Y

A

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

A

A

A

G

G

G

P

P

L

R

H

E

L

L

I

I

K

K

A

A

M

M

D

A

M

V

I

V

Q

Q

G

S

Q

Q

Q

A

T

T

S

S

G

C

A

P

A

S

S

S

I

I

A

S

Q

Q

W

A

A

A

A

S

V

V

E

V

A

A

L

L

N

N

G

G

P

P

Q

Q

D

D

F

F

I

L

G

K

R

E

N

R

K

T

E

E

I

S

F

F

Q

Q

G

R

R

R

R

R

D

D

L

L

V

V

V

V

S

N

M

G

L

L

N

N

Q

A

A

I

K

D

G

G

I

L

S

D

C

C

P

R

T

V

P

P

E

E

G

G

A

A

F

F

Y

Y

V

T

Y

F

P

S

S

G

C

C

A

A

G

G

L

V

I

L

G

G

K

K

T

V

A

T

P

P

S

S

G

G

K

K

V

R

I

I

E

K

T

T

D

D

E

T

D

D

F

F

V

C

S

A

E

Y

L

L

L

L

E

E

T

D

E

A

G

H

V

V

A

A

V

V

V

V

H

P

G

G

S

S

A

A

F

F

G

G

L

L

G

S

P

P

N

F

F

F

R

R

I

I

S

S

Y

Y

A

A

T

T

S

S

E

E

A

A

L

E

L

L

E

K

E

E

A

A

C

L

R

E

R

R

I

I

Q

A

R

A

F

A

C

C

binding pyridoxal-5'-phosphate: G99 (= G99), A100 (≠ G100), K101 (= K101), D204 (= D204), M206 (= M206), Y207 (= Y207), S238 (= S238), K239 (= K239), R247 (= R247)

8wkjA The crystal structure of aspartate aminotransferases lpg0070 from legionella pneumophila (see paper)
53% identity, 99% coverage: 4:397/400 of query aligns to 5:391/391 of 8wkjA

query
sites
8wkjA

L

L

A

A

D

K

A

R

L

V

S

Q

R

K

V

V

K

K

P

-

S

S

A

P

T

T

I

L

A

A

V

V

S

A

Q

A

K

K

A

A

R

A

E

Q

L

M

K

K

A

A

K

Q

G

G

R

L

D

D

V

I

I

I

G

G

L

L

G

G

A

T

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

D

Q

N

H

I

I

K

K

K

L

A

A

A

A

I

I

D

S

A

A

I

I

D

E

R

A

G

G

E

D

T

T

K

K

Y

Y

T

T

P

A

V

V

S

D

G

G

I

I

P

V

E

E

L

L

R

K

E

E

A

A

I

V

A

K

K

N

K

K

F

F

K

K

R

R

E

D

N

N

N

E

L

L

D

D

Y

Y

T

Q

A

L

A

N

Q

Q

T

I

I

L

V

V

G

S

T

V

G
|
G

G
|
G

K
|
K

Q

Q

I

S

L

C

F

Y

N

N

A

L

F

C

M

Q

A

A

T

Y

L

L

N

N

P

P

G

G

D

D

E

E

V

V

V

I

I

I

P

P

A

A

P

P

Y

Y

W
|
W

V

V

S

S

Y

Y

P

P

E

D

M

M

V

V

A

L

L

L

C

A

G

D

G

G

T

V

P

P

V

V

F

I

V

I

P

E

T

T

R

T

Q

P

E

A

N

Q

N

R

F

Y

K

K

L

I

K

N

A

A

E

Q

D

Q

L

L

D

E

R

Q

A

A

I

I

T

T

P

P

K

K

T

T

K

R

W

M

F

I

V

F

F

L

N
|
N

S

S

P

P

S

S

N
|
N

P

P

S

S

G

G

A

I

A

A

Y

Y

S

T

H

Q

E

N

E

E

L

L

K

K

A

E

L

L

T

G

D

D

V

V

L

L

M

K

K

K

H

H

P

P

H

Q

V

I

W

L

V

I

L

A

T

T

D
|
D

D

D

M

M

Y
|
Y

E

E

H

H

L

I

T

I

Y

W

G

S

D

Q

F

-

R

P

F

F

A

T

T

N

P

I

V

L

E

N

V

A

E

C

P

P

G

E

L

L

Y

Y

E

D

R

R

T

T

L

I

T

V

M

L

N

N

G

G

V

V

S
|
S

K
|
K

A

A

Y

Y

A

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

A

A

A

A

G

G

P

P

L

A

H

P

L

L

I

I

K

N

A

A

M

M

D

K

M

T

I

I

Q

Q

G

S

Q

Q

Q

S

T

T

S

S

G

N

A

P

A

C

S

S

I

I

A

A

Q

Q

W

R

A

A

A

A

V

V

E

A

A

A

L

L

N

N

G

G

P

S

Q

N

D

E

F

S

I

I

G

E

R

E

N

M

K

V

E

N

I

A

F

F

Q

H

G

Q

R

R

R

H

D

D

L

Y

V

V

V

A

S

D

M

R

L

L

N

Q

Q

S

A

I

K

D

G

G

I

I

S

E

C

V

P

I

T

P

P

A

E

D

G

G

A

T

F

F

Y

Y

V

I

Y

F

P

P

S

S

C

V

A

Q

G

A

L

I

I

I

G

E

K

K

T

R

A

G

P

Y

S

A

G

-

K

-

V

-

I

-

E

-

T

N

D

D

E

I

D

E

F

F

V

S

S

E

E

K

L

L

L

L

E

N

T

E

E

V

G

G

V

V

A

A

V

L

V

V

H

P

G

G

S

S

A

A

F

F

G

G

L

T

G

E

P

G

N

C

F

I

R

R

I

I

S

S

Y

F

A

A

T

T

S

G

E

I

A

D

L

T

L

L

E

K

E

D

A

A

C

L

R

N

R

R

I

L

Q

Q

R

R

F

F

C

C

A

S

binding pyridoxal-5'-phosphate: G99 (= G99), G100 (= G100), K101 (= K101), W125 (= W125), N171 (= N171), N175 (= N175), D204 (= D204), Y207 (= Y207), S237 (= S238), K238 (= K239), R246 (= R247)

1j32A Aspartate aminotransferase from phormidium lapideum
45% identity, 98% coverage: 4:395/400 of query aligns to 3:383/388 of 1j32A

query
sites
1j32A

L

L

A

A

D

A

A

R

L

V

S

E

R

S

V

V

K

S

P

P

S

S

A

M

T

T

I

L

A

I

V

I

S

D

Q

A

K

K

A

A

R

K

E

A

L

M

K

K

A

A

K

E

G

G

R

I

D

D

V

V

I

C

G

S

L

F

G

S

A

A

G

G

E

E

P

P

D

D

F

F

D

N

T

T

P

P

D

K

N

H

I

I

K

V

K

E

A

A

A

A

I

K

D

A

A

A

I

L

D

E

R

Q

G

G

E

K

T

T

K

R

Y

Y

T

G

P

P

V

A

S

A

G

G

I

E

P

P

E

R

L

L

R

R

E

E

A

A

I

I

A

A

K

Q

K

K

F

L

K

Q

R

R

E

D

N

N

N

G

L

L

D

C

Y

Y

T

G

A

A

A

D

Q

N

T

I

I

L

V

V

G

T

T

N

G
|
G

G
|
G

K
|
K

Q

Q

I

S

L

I

F

F

N

N

A

L

F

M

M

L

A

A

T

M

L

I

N

E

P

P

G

G

D

D

E

E

V

V

V

I

I

I

P

P

A

A

P

P

Y

F

W
|
W

V

V

S

S

Y

Y

P

P

E

E

M

M

V

V

A

K

L

L

C

A

G

E

G

G

T

T

P

P

V

V

F

I

V

L

P

P

T

T

R

T

Q

V

E

E

N

T

N

Q

F

F

K

K

L

V

K

S

A

P

E

E

D

Q

L

I

D

R

R

Q

A

A

I

I

T

T

P

P

K

K

T

T

K

K

W

L

F

L

V

V

F

F

N

N

S

T

P

P

S

S

N
|
N

P

P

S

T

G

G

A

M

A

V

Y

Y

S

T

H

P

E

D

E

E

L

V

K

R

A

A

L

I

T

A

D

Q

V

V

L

A

M

V

K

E

H

A

P

-

H

G

V

L

W

W

V

V

L

L

T

S

D
|
D

D

E

M
x
I

Y
|
Y

E

E

H

K

L

I

T

L

Y

Y

G

D

D

D

F

A

R

Q

F

H

A

L

T

S

P

I

V

G

E

A

V

A

E

S

P

P

G

E

L

A

Y

Y

E

E

R

R

T

S

L

V

T

V

M

C

N

S

G

G

V

F

S

A

K
|
K

A

T

Y

Y

A

A

M

M

T

T

G

G

W

W

R
|
R

I

V

G

G

Y

F

A

L

A

A

G

G

P

P

L

V

H

P

L

L

I

V

K

K

A

A

M

A

D

T

M

K

I

I

Q

Q

G

G

Q

H

Q

S

T

T

S

S

G

N

A

V

A

C

S

T

I

F

A

A

Q

Q

W

Y

A

G

A

A

V

I

E

A

A

A

L

Y

N

E

G

N

P

S

Q

Q

D

D

F

C

I

V

G

Q

R

E

N

M

K

L

E

A

I

A

F

F

Q

A

G

E

R

R

R

R

D

R

L

Y

V

M

V

L

S

D

M

A

L

L

N

N

Q

A

A

M

K

P

G

G

I

L

S

E

C

C

P

P

T

K

P

P

E

D

G

G

A

A

F

F

Y

Y

V

M

Y

F

P

P

S

S

C

-

A

-

G

-

L

-

I

I

G

A

K

K

T

T

A

G

P

R

S

S

G

S

K

L

V

-

I

-

E

-

T

-

D

-

E

-

D

D

F

F

V

C

S

S

E

E

L

L

L

L

E

D

T

Q

E

H

G

Q

V

V

A

A

V

T

V

V

H

P

G

G

S

A

A

A

F

F

G

G

L

A

G

D

P

D

N

C

F

I

R

R

I

L

S

S

Y

Y

A

A

T

T

S

D

E

L

A

D

L

T

L

I

E

K

E

R

A

G

C

M

R

E

R

R

I

L

Q

E

R

K

F

F

active site: W124 (= W125), D202 (= D204), I204 (≠ M206), K237 (= K239)
binding pyridoxal-5'-phosphate: G98 (= G99), G99 (= G100), K100 (= K101), W124 (= W125), N174 (= N175), D202 (= D204), I204 (≠ M206), Y205 (= Y207), K237 (= K239), R245 (= R247)

Q56232 Aspartate/prephenate aminotransferase; AspAT / PAT; Transaminase A; EC 2.6.1.1; EC 2.6.1.78 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see 3 papers)
48% identity, 99% coverage: 1:396/400 of query aligns to 1:382/385 of Q56232

query
sites
Q56232

M

M

A

R

F

G

L

L

A

S

D

R

A

R

L

V

S

Q

R

A

V

M

K
|
K

P

P

S

S

A

A

T

T

I

V

A

A

V

V

S

N

Q

A

K

K

A

A

R

L

E

E

L

L

K

R

A

R

K

Q

G

G

R

V

D

D

V

L

I

V

G

A

L

L

G

T

A

A

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

D

E

N

H

I

V

K

K

K

E

A

A

A

A

I

R

D

R

A

A

I

L

D

A

R

Q

G

G

E

K

T

T

K

K

Y

Y

T

A

P

P

V

P

S

A

G

G

I

I

P

P

E

E

L

L

R

R

E

E

A

A

I

L

A

A

K

E

K

K

F

F

K

R

R

R

E

E

N

N

N

G

L

L

D

S

Y

V

T

T

A

P

A

E

Q

E

T

T

I

I

V

V

G

T

T

V

G

G

G

G

K

K

Q

Q

I

A

L

L

F

F

N

N

A

L

F

F

M

Q

A

A

T

I

L

L

N

D

P

P

G

G

D

D

E

E

V

V

V

I

I

V

P

L

A

S

P

P

Y

Y

W

W

V

V

S

S

Y

Y

P

P

E

E

M

M

V

V

A

R

L

F

C

A

G

G

G

G

T

V

P

V

V

V

F

E

V

V

P

E

T

T

R

L

Q

P

E

E

N

E

N

G

F

F

K

V

L

P

K

D

A

P

E

E

D

R

L

V

D

R

R

R

A

A

I

I

T

T

P

P

K

R

T

T

K

K

W

A

F

L

V

V

F

V

N

N

S

S

P

P

S

N

N

N

P

P

S

T

G

G

A

A

A

V

Y

Y

S

P

H

K

E

E

E

V

L

L

K

E

A

A

L

L

T

A

D

R

V

L

L

A

M

V

K

E

H

H

P

-

H

D

V

F

W

Y

V

L

L

V

T

S

D

D

D

E

M

I

Y

Y

E

E

H

H

L

L

T

L

Y

Y

G

E

D

G

F

E

R

H

F

F

A

-

T

S

P

P

V

G

E

R

V

V

E

A

P

P

G

-

L

-

Y

-

E

E

R

H

T

T

L

L

T

T

M

V

N

N

G

G

V

A

S

A

K
|
K

A

A

Y

F

A

A

M

M

T

T

G

G

W

W

R

R

I

I

G

G

Y

Y

A

A

A

C

G

G

P

P

L

K

H

E

L

V

I

I

K

K

A

A

M

M

D

A

M

S

I

V

Q

S

G

S

Q

Q

Q

S

T

T

S

T

G

S

A

P

A

D

S

T

I

I

A

A

Q

Q

W

W

A

A

A

T

V

L

E

E

A

A

L

L

N

T

-

N

-

Q

-

E

G

A

P

S

Q

R

D

A

F

F

I

V

G

E

R

M

N

A

K

R

E

E

I

A

F

Y

Q

R

G

R

R

R

R

R

D

D

L

L

V

L

V

L

S

E

M

G

L

L

N

T

Q

-

A

A

K

L

G

G

I

L

S

K

C

A

P

V

T

R

P

P

E

S

G

G

A

A

F

F

Y

Y

V

V

Y

-

P

-

S

-

C

-

A

-

G

-

L

-

I

L

G

M

K

D

T

T

A

S

P

P

S

-

G

-

K

-

V

-

I

I

E

A

T

P

D

D

E

E

D

V

F

R

V

A

S

A

E

E

L

R

L

L

E

L

T

E

E

A

G

G

V

V

A

A

V

V

V

V

H

P

G

G

S

T

A

D

F

F

G

A

L

A

G

F

P

G

N

H

F

V

R

R

I

L

S

S

Y

Y

A

A

T

T

S

S

E

E

A

E

L

N

L

L

E

R

E

K

A

A

C

L

R

E

R

R

I

F

Q

A

R

R

F

V

C

L

K12 (= K12) Important for prephenate aminotransferase activity; mutation to G: 10-fold increase in Km for prephenate. Does not affect Km for oxaloacetate.
K234 (= K239) modified: N6-(pyridoxal phosphate)lysine

1bkgA Aspartate aminotransferase from thermus thermophilus with maleate (see paper)
48% identity, 99% coverage: 1:396/400 of query aligns to 1:382/382 of 1bkgA

query
sites
1bkgA

M

M

A

R

F

G

L

L

A

S

D

R

A

R

L

V

S

Q

R

A

V

M

K

K

P

P

S

S

A

A

T

T

I

V

A

A

V

V

S

N

Q

A

K

K

A

A

R

L

E

E

L

L

K

R

A

R

K

Q

G

G

R

V

D

D

V

L

I

V

G

A

L

L

G

T

A

A

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

D

E

N

H

I

V

K

K

K

E

A

A

A

A

I

R

D

R

A

A

I

L

D

A

R

Q

G

G

E

K

T

T

K

K

Y

Y

T

A

P

P

V

P

S

A

G

G

I

I

P

P

E

E

L

L

R

R

E

E

A

A

I

L

A

A

K

E

K

K

F

F

K

R

R

R

E

E

N

N

N

G

L

L

D

S

Y

V

T

T

A

P

A

E

Q

E

T

T

I

I

V

V

G

T

T

V

G
|
G

G
|
G

K
|
K

Q

Q

I

A

L

L

F

F

N

N

A

L

F

F

M

Q

A

A

T

I

L

L

N

D

P

P

G

G

D

D

E

E

V

V

V

I

I

V

P

L

A

S

P

P

Y

Y

W
|
W

V

V

S

S

Y

Y

P

P

E

E

M

M

V

V

A

R

L

F

C

A

G

G

G

G

T

V

P

V

V

V

F

E

V

V

P

E

T

T

R

L

Q

P

E

E

N

E

N

G

F

F

K

V

L

P

K

D

A

P

E

E

D

R

L

V

D

R

R

R

A

A

I

I

T

T

P

P

K

R

T

T

K

K

W

A

F

L

V

V

F

V

N
|
N

S

S

P

P

S

N

N
|
N

P

P

S

T

G

G

A

A

A

V

Y

Y

S

P

H

K

E

E

E

V

L

L

K

E

A

A

L

L

T

A

D

R

V

L

L

A

M

V

K

E

H

H

P

-

H

D

V

F

W

Y

V

L

L

V

T

S

D
|
D

D

E

M
x
I

Y
|
Y

E

E

H

H

L

L

T

L

Y

Y

G

E

D

G

F

E

R

H

F

F

A

-

T

S

P

P

V

G

E

R

V

V

E

A

P

P

G

-

L

-

Y

-

E

E

R

H

T

T

L

L

T

T

M

V

N

N

G

G

V

A

S

A

K
|
K

A

A

Y

F

A

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

A

A

A

C

G

G

P

P

L

K

H

E

L

V

I

I

K

K

A

A

M

M

D

A

M

S

I

V

Q

S

G

S

Q

Q

Q

S

T

T

S

T

G

S

A

P

A

D

S

T

I

I

A

A

Q

Q

W

W

A

A

A

T

V

L

E

E

A

A

L

L

N

T

-

N

-

Q

-

E

G

A

P

S

Q

R

D

A

F

F

I

V

G

E

R

M

N

A

K

R

E

E

I

A

F

Y

Q

R

G

R

R

R

R

R

D

D

L

L

V

L

V

L

S

E

M

G

L

L

N

T

Q

-

A

A

K

L

G

G

I

L

S

K

C

A

P

V

T

R

P

P

E

S

G

G

A

A

F

F

Y

Y

V

V

Y

-

P

-

S

-

C

-

A

-

G

-

L

-

I

L

G

M

K

D

T

T

A

S

P

P

S

-

G

-

K

-

V

-

I

I

E

A

T

P

D

D

E

E

D

V

F

R

V

A

S

A

E

E

L

R

L

L

E

L

T

E

E

A

G

G

V

V

A

A

V

V

V

V

H

P

G

G

S

T

A

D

F

F

G

A

L

A

G

F

P

G

N

H

F

V

R

R

I

L

S

S

Y

Y

A

A

T

T

S

S

E

E

A

E

L

N

L

L

E

R

E

K

A

A

C

L

R

E

R

R

I

F

Q

A

R

R

F

V

C

L

active site: W125 (= W125), D203 (= D204), I205 (≠ M206), K234 (= K239)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G99 (= G99), G100 (= G100), K101 (= K101), W125 (= W125), N171 (= N171), N175 (= N175), D203 (= D204), I205 (≠ M206), Y206 (= Y207), K234 (= K239), R242 (= R247)

1bjwA Aspartate aminotransferase from thermus thermophilus (see paper)
48% identity, 99% coverage: 1:396/400 of query aligns to 1:382/382 of 1bjwA

query
sites
1bjwA

M

M

A

R

F

G

L

L

A

S

D

R

A

R

L

V

S

Q

R

A

V

M

K

K

P

P

S

S

A

A

T

T

I

V

A

A

V

V

S

N

Q

A

K

K

A

A

R

L

E

E

L

L

K

R

A

R

K

Q

G

G

R

V

D

D

V

L

I

V

G

A

L

L

G

T

A

A

G
|
G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

D

E

N

H

I

V

K

K

K

E

A

A

A

A

I

R

D

R

A

A

I

L

D

A

R

Q

G

G

E

K

T

T

K

K

Y

Y

T

A

P

P

V

P

S

A

G

G

I

I

P

P

E

E

L

L

R

R

E

E

A

A

I

L

A

A

K

E

K

K

F

F

K

R

R

R

E

E

N

N

N

G

L

L

D

S

Y

V

T

T

A

P

A

E

Q

E

T

T

I

I

V

V

G

T

T

V

G

G

G

G

K

K

Q

Q

I

A

L

L

F

F

N

N

A

L

F

F

M

Q

A

A

T

I

L

L

N

D

P

P

G

G

D

D

E

E

V

V

V

I

I

V

P

L

A

S

P

P

Y

Y

W
|
W

V

V

S

S

Y

Y

P

P

E

E

M

M

V

V

A

R

L

F

C

A

G

G

G

G

T

V

P

V

V

V

F

E

V

V

P

E

T

T

R

L

Q

P

E

E

N

E

N

G

F

F

K

V

L

P

K

D

A

P

E

E

D

R

L

V

D

R

R

R

A

A

I

I

T

T

P

P

K

R

T

T

K

K

W

A

F

L

V

V

F

V

N

N

S

S

P

P

S

N

N
|
N

P

P

S

T

G

G

A

A

A

V

Y

Y

S

P

H

K

E

E

E

V

L

L

K

E

A

A

L

L

T

A

D

R

V

L

L

A

M

V

K

E

H

H

P

-

H

D

V

F

W

Y

V

L

L

V

T

S

D
|
D

D

E

M
x
I

Y

Y

E

E

H

H

L

L

T

L

Y

Y

G

E

D

G

F

E

R

H

F

F

A

-

T

S

P

P

V

G

E

R

V

V

E

A

P

P

G

-

L

-

Y

-

E

E

R

H

T

T

L

L

T

T

M

V

N

N

G

G

V

A

S

A

K
|
K

A

A

Y

F

A

A

M

M

T

T

G

G

W

W

R

R

I

I

G

G

Y

Y

A

A

A

C

G

G

P

P

L

K

H

E

L

V

I

I

K

K

A

A

M

M

D

A

M

S

I

V

Q

S

G

S

Q

Q

Q

S

T

T

S

T

G

S

A

P

A

D

S

T

I

I

A

A

Q

Q

W

W

A

A

A

T

V

L

E

E

A

A

L

L

N

T

-

N

-

Q

-

E

G

A

P

S

Q

R

D

A

F

F

I

V

G

E

R

M

N

A

K

R

E

E

I

A

F

Y

Q

R

G

R

R

R

R

R

D

D

L

L

V

L

V

L

S

E

M

G

L

L

N

T

Q

-

A

A

K

L

G

G

I

L

S

K

C

A

P

V

T

R

P

P

E

S

G

G

A

A

F

F

Y

Y

V

V

Y

-

P

-

S

-

C

-

A

-

G

-

L

-

I

L

G

M

K

D

T

T

A

S

P

P

S

-

G

-

K

-

V

-

I

I

E

A

T

P

D

D

E

E

D

V

F

R

V

A

S

A

E

E

L

R

L

L

E

L

T

E

E

A

G

G

V

V

A

A

V

V

V

V

H

P

G

G

S

T

A

D

F

F

G

A

L

A

G

F

P

G

N

H

F

V

R
|
R

I

L

S

S

Y

Y

A

A

T

T

S

S

E

E

A

E

L

N

L

L

E

R

E

K

A

A

C

L

R

E

R

R

I

F

Q

A

R

R

F

V

C

L

active site: W125 (= W125), D203 (= D204), I205 (≠ M206), K234 (= K239)
binding phosphate ion: G39 (= G39), W125 (= W125), N175 (= N175), R361 (= R375)

1b5oA Thermus thermophilus aspartate aminotransferase single mutant 1 (see paper)
48% identity, 99% coverage: 1:396/400 of query aligns to 1:382/382 of 1b5oA

query
sites
1b5oA

M

M

A

R

F

G

L

L

A

S

D

R

A

R

L

V

S

Q

R

A

V

M

K

K

P

P

S

S

A

A

T

T

I

V

A

A

V

V

S

N

Q

A

K

K

A

A

R

L

E

E

L

L

K

R

A

R

K

Q

G

G

R

V

D

D

V

L

I

V

G

A

L

L

G

T

A

A

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

D

E

N

H

I

V

K

K

K

E

A

A

A

A

I

R

D

R

A

A

I

L

D

A

R

Q

G

G

E

K

T

T

K

K

Y

Y

T

A

P

P

V

P

S

A

G

G

I

I

P

P

E

E

L

L

R

R

E

E

A

A

I

L

A

A

K

E

K

K

F

F

K

R

R

R

E

E

N

N

N

G

L

L

D

S

Y

V

T

T

A

P

A

E

Q

E

T

T

I

I

V

V

G

T

T

V

G
|
G

G
|
G

K
x
S

Q

Q

I

A

L

L

F

F

N

N

A

L

F

F

M

Q

A

A

T

I

L

L

N

D

P

P

G

G

D

D

E

E

V

V

V

I

I

V

P

L

A

S

P

P

Y

Y

W
|
W

V

V

S

S

Y

Y

P

P

E

E

M

M

V

V

A

R

L

F

C

A

G

G

G

G

T

V

P

V

V

V

F

E

V

V

P

E

T

T

R

L

Q

P

E

E

N

E

N

G

F

F

K

V

L

P

K

D

A

P

E

E

D

R

L

V

D

R

R

R

A

A

I

I

T

T

P

P

K

R

T

T

K

K

W

A

F

L

V

V

F

V

N

N

S

S

P

P

S

N

N
|
N

P

P

S

T

G

G

A

A

A

V

Y

Y

S

P

H

K

E

E

E

V

L

L

K

E

A

A

L

L

T

A

D

R

V

L

L

A

M

V

K

E

H

H

P

-

H

D

V

F

W

Y

V

L

L

V

T

S

D
|
D

D

E

M
x
I

Y
|
Y

E

E

H

H

L

L

T

L

Y

Y

G

E

D

G

F

E

R

H

F

F

A

-

T

S

P

P

V

G

E

R

V

V

E

A

P

P

G

-

L

-

Y

-

E

E

R

H

T

T

L

L

T

T

M

V

N

N

G

G

V

A

S
x
A

K
|
K

A

A

Y

F

A

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

A

A

A

C

G

G

P

P

L

K

H

E

L

V

I

I

K

K

A

A

M

M

D

A

M

S

I

V

Q

S

G

S

Q

Q

Q

S

T

T

S

T

G

S

A

P

A

D

S

T

I

I

A

A

Q

Q

W

W

A

A

A

T

V

L

E

E

A

A

L

L

N

T

-

N

-

Q

-

E

G

A

P

S

Q

R

D

A

F

F

I

V

G

E

R

M

N

A

K

R

E

E

I

A

F

Y

Q

R

G

R

R

R

R

R

D

D

L

L

V

L

V

L

S

E

M

G

L

L

N

T

Q

-

A

A

K

L

G

G

I

L

S

K

C

A

P

V

T

R

P

P

E

S

G

G

A

A

F

F

Y

Y

V

V

Y

-

P

-

S

-

C

-

A

-

G

-

L

-

I

L

G

M

K

D

T

T

A

S

P

P

S

-

G

-

K

-

V

-

I

I

E

A

T

P

D

D

E

E

D

V

F

R

V

A

S

A

E

E

L

R

L

L

E

L

T

E

E

A

G

G

V

V

A

A

V

V

V

V

H

P

G

G

S

T

A

D

F

F

G

A

L

A

G

F

P

G

N

H

F

V

R

R

I

L

S

S

Y

Y

A

A

T

T

S

S

E

E

A

E

L

N

L

L

E

R

E

K

A

A

C

L

R

E

R

R

I

F

Q

A

R

R

F

V

C

L

active site: W125 (= W125), D203 (= D204), I205 (≠ M206), K234 (= K239)
binding pyridoxal-5'-phosphate: G99 (= G99), G100 (= G100), S101 (≠ K101), W125 (= W125), N175 (= N175), D203 (= D204), I205 (≠ M206), Y206 (= Y207), A233 (≠ S238), K234 (= K239), R242 (= R247)

1gc4A Thermus thermophilus aspartate aminotransferase tetra mutant 2 complexed with aspartate (see paper)
47% identity, 99% coverage: 1:396/400 of query aligns to 1:382/382 of 1gc4A

query
sites
1gc4A

M

M

A

R

F

G

L

L

A

S

D

R

A

R

L

V

S

Q

R

A

V

M

K

K

P

P

S

D

A

A

T

V

I

V

A

A

V

V

S

N

Q

A

K

K

A

A

R

L

E

E

L

L

K

R

A

R

K

Q

G

G

R

V

D

D

V

L

I

V

G

A

L

L

G

T

A

A

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

D

E

N

H

I

V

K

K

K

E

A

A

A

A

I

R

D

R

A

A

I

L

D

A

R

Q

G

G

E

K

T

T

K

K

Y
|
Y

T

A

P

P

V

P

S

A

G

G

I

I

P

P

E

E

L

L

R

R

E

E

A

A

I

L

A

A

K

E

K

K

F

F

K

R

R

R

E

E

N

N

N

G

L

L

D

S

Y

V

T

T

A

P

A

E

Q

E

T

T

I

I

V

V

G

T

T

V

G
|
G

G
|
G

K
x
S

Q

Q

I

A

L

L

F

F

N

N

A

L

F

F

M

Q

A

A

T

I

L

L

N

D

P

P

G

G

D

D

E

E

V

V

V

I

I

V

P

L

A

S

P

P

Y

Y

W
|
W

V

V

S

S

Y

Y

P

P

E

E

M

M

V

V

A

R

L

F

C

A

G

G

G

G

T

V

P

V

V

V

F

E

V

V

P

E

T

T

R

L

Q

P

E

E

N

E

N

G

F

F

K

V

L

P

K

D

A

P

E

E

D

R

L

V

D

R

R

R

A

A

I

I

T

T

P

P

K

R

T

T

K

K

W

A

F

L

V

V

F

V

N

N

S

S

P

P

S

N

N
|
N

P

P

S

T

G

G

A

A

A

V

Y

Y

S

P

H

K

E

E

E

V

L

L

K

E

A

A

L

L

T

A

D

R

V

L

L

A

M

V

K

E

H

H

P

-

H

D

V

F

W

Y

V

L

L

V

T

S

D
|
D

D

E

M

I

Y
|
Y

E

E

H

H

L

L

T

L

Y

Y

G

E

D

G

F

E

R

H

F

F

A

-

T

S

P

P

V

G

E

R

V

V

E

A

P

P

G

-

L

-

Y

-

E

E

R

H

T

T

L

L

T

T

M

V

N

N

G

G

V

A

S

A

K

K

A

A

Y

F

A

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

A

A

A

C

G

G

P

P

L

K

H

E

L

V

I

I

K

K

A

A

M

M

D

A

M

S

I

V

Q

S

G
x
R

Q

Q

Q

S

T

T

S

T

G

S

A

P

A

D

S

T

I

I

A

A

Q

Q

W

W

A

A

A

T

V

L

E

E

A

A

L

L

N

T

-

N

-

Q

-

E

G

A

P

S

Q

R

D

A

F

F

I

V

G

E

R

M

N

A

K

R

E

E

I

A

F

Y

Q

R

G

R

R

R

R

R

D

D

L

L

V

L

V

L

S

E

M

G

L

L

N

T

Q

-

A

A

K

L

G

G

I

L

S

K

C

A

P

V

T

R

P

P

E

S

G

G

A

A

F

F

Y
|
Y

V

V

Y

-

P

-

S

-

C

-

A

-

G

-

L

-

I

L

G

M

K

D

T

T

A

S

P

P

S

-

G

-

K

-

V

-

I

I

E

A

T

P

D

D

E

E

D

V

F

R

V

A

S

A

E

E

L

R

L

L

E

L

T

E

E

A

G

G

V

V

A

A

V

V

V

V

H

P

G

G

S

T

A

D

F

F

G

A

L

A

G

F

P

G

N

H

F

V

R
|
R

I

L

S

S

Y

Y

A

A

T

T

S

S

E

E

A

E

L

N

L

L

E

R

E

K

A

A

C

L

R

E

R

R

I

F

Q

A

R

R

F

V

C

L

binding aspartic acid: Y64 (= Y64), W125 (= W125), N175 (= N175), R261 (≠ G266), Y322 (= Y325), R361 (= R375)
binding pyridoxal-5'-phosphate: G99 (= G99), G100 (= G100), S101 (≠ K101), W125 (= W125), N175 (= N175), D203 (= D204), Y206 (= Y207), R242 (= R247)

1gc3A Thermus thermophilus aspartate aminotransferase tetra mutant 2 complexed with tryptophan (see paper)
47% identity, 99% coverage: 1:396/400 of query aligns to 1:382/382 of 1gc3A

query
sites
1gc3A

M

M

A

R

F

G

L

L

A

S

D

R

A

R

L

V

S

Q

R

A

V

M

K

K

P

P

S

D

A

A

T
x
V

I

V

A

A

V

V

S
x
N

Q

A

K

K

A

A

R

L

E

E

L

L

K

R

A

R

K

Q

G

G

R

V

D

D

V

L

I

V

G

A

L

L

G
x
T

A
|
A

G
|
G

E
|
E

P

P

D

D

F

F

D

D

T

T

P

P

D

E

N

H

I

V

K

K

K

E

A

A

A

A

I

R

D

R

A

A

I

L

D

A

R

Q

G

G

E

K

T

T

K

K

Y

Y

T

A

P

P

V

P

S

A

G

G

I

I

P

P

E

E

L

L

R

R

E

E

A

A

I

L

A

A

K

E

K

K

F

F

K

R

R

R

E

E

N

N

N

G

L

L

D

S

Y

V

T

T

A

P

A

E

Q

E

T

T

I

I

V

V

G

T

T

V

G

G

G
|
G

K
x
S

Q

Q

I

A

L

L

F

F

N

N

A

L

F

F

M

Q

A

A

T

I

L

L

N

D

P

P

G

G

D

D

E

E

V

V

V

I

I

V

P

L

A

S

P

P

Y

Y

W
|
W

V

V

S

S

Y

Y

P

P

E

E

M

M

V

V

A

R

L

F

C

A

G

G

G

G

T

V

P

V

V

V

F

E

V

V

P

E

T

T

R

L

Q

P

E

E

N

E

N

G

F

F

K

V

L

P

K

D

A

P

E

E

D

R

L

V

D

R

R

R

A

A

I

I

T

T

P

P

K

R

T

T

K

K

W

A

F

L

V

V

F

V

N

N

S

S

P

P

S

N

N
|
N

P

P

S

T

G

G

A

A

A

V

Y

Y

S

P

H

K

E

E

E

V

L

L

K

E

A

A

L

L

T

A

D

R

V

L

L

A

M

V

K

E

H

H

P

-

H

D

V

F

W

Y

V

L

L

V

T

S

D
|
D

D

E

M

I

Y
|
Y

E

E

H

H

L

L

T

L

Y

Y

G

E

D

G

F

E

R

H

F

F

A

-

T

S

P

P

V

G

E

R

V

V

E

A

P

P

G

-

L

-

Y

-

E

E

R

H

T

T

L

L

T

T

M

V

N

N

G

G

V<