SitesBLAST
Comparing SMc01662 FitnessBrowser__Smeli:SMc01662 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
Q8GAI3 4-methylaminobutanoate oxidase (formaldehyde-forming); MABO; Demethylating gamma-N-methylaminobutyrate oxidase; Gamma-N-methylaminobutyrate oxidase 1; EC 1.5.3.19 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
42% identity, 100% coverage: 4:814/815 of query aligns to 21:823/824 of Q8GAI3
- W66 (= W49) mutation W->F,S: Contains a non-covalently bound FAD. Loss of enzyme activity.
- H67 (= H50) mutation to A: Contains a non-covalently bound FAD. Exhibits about 10% of the wild-type enzyme activity.
Q63342 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Rattus norvegicus (Rat) (see 2 papers)
34% identity, 99% coverage: 8:814/815 of query aligns to 42:849/857 of Q63342
4pabB Crystal structure of the precursor form of rat dmgdh complexed with tetrahydrofolate (see paper)
34% identity, 99% coverage: 8:814/815 of query aligns to 5:812/824 of 4pabB
- active site: T53 (≠ G56), E102 (= E105), H226 (= H233), Y255 (= Y258), E536 (≠ D537)
- binding flavin-adenine dinucleotide: I11 (= I14), G12 (= G15), G14 (= G17), C15 (≠ V18), V16 (≠ S19), L35 (= L38), E36 (= E39), K37 (≠ R40), G43 (= G46), S44 (≠ T47), T45 (= T48), H47 (= H50), A48 (= A51), A49 (= A52), G50 (= G53), L51 (= L54), V175 (= V178), A204 (= A211), G205 (= G212), W207 (= W214), H226 (= H233), Y228 (= Y235), G326 (= G324), I328 (≠ E326), F353 (≠ Y351), Y355 (≠ I354), G356 (= G355), I357 (= I356), I358 (≠ V357)
- binding (6s)-5,6,7,8-tetrahydrofolate: I523 (≠ Y524), E536 (≠ D537), T538 (= T539), I550 (≠ V551), F612 (= F611), L613 (≠ A612), Y632 (= Y631), E639 (= E638), F680 (= F679), Y700 (≠ F699)
Q9UI17 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Homo sapiens (Human) (see 4 papers)
33% identity, 99% coverage: 8:814/815 of query aligns to 49:856/866 of Q9UI17
- CV 59:60 (≠ VS 18:19) binding
- EK 80:81 (≠ ER 39:40) binding
- 87:95 (vs. 46:54, 89% identical) binding
- H91 (= H50) modified: Tele-8alpha-FAD histidine
- H109 (≠ A68) to R: in DMGDHD; shows 10 fold lower catalytic efficiency due to lower cofactor saturation and reduced thermal stability; dbSNP:rs121908331
- V219 (= V178) binding
- S279 (≠ P242) to P: in dbSNP:rs532964
- F-GYGII 397:402 (≠ YNSIGIV 351:357) binding
- A530 (≠ G486) to G: in dbSNP:rs1805073
- S646 (≠ P601) to P: in dbSNP:rs1805074
1pj7A Structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folinic acid (see paper)
32% identity, 99% coverage: 10:814/815 of query aligns to 3:826/827 of 1pj7A
- active site: H222 (= H233), Y256 (= Y258), D549 (= D537)
- binding flavin-adenine dinucleotide: G8 (= G15), G10 (= G17), I11 (≠ V18), V12 (≠ S19), D32 (≠ E39), Q33 (≠ R40), G41 (= G46), S42 (≠ T47), T43 (= T48), H45 (= H50), P47 (≠ A52), L49 (= L54), T170 (≠ K177), V171 (= V178), A200 (= A211), G201 (= G212), W203 (= W214), H222 (= H233), Y256 (= Y258), I331 (≠ P325), V357 (≠ I354), W358 (≠ G355), V359 (≠ I356), T360 (≠ V357)
- binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: L505 (≠ F493), Y536 (= Y524), D549 (= D537), T551 (= T539), G563 (= G554), F629 (≠ A612), Y648 (= Y631), E655 (= E638), Y696 (≠ F679)
1pj6A Crystal structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folic acid (see paper)
32% identity, 99% coverage: 10:814/815 of query aligns to 4:827/828 of 1pj6A
- active site: H223 (= H233), Y257 (= Y258), D550 (= D537)
- binding flavin-adenine dinucleotide: G9 (= G15), G11 (= G17), I12 (≠ V18), V13 (≠ S19), D33 (≠ E39), Q34 (≠ R40), G42 (= G46), S43 (≠ T47), T44 (= T48), H46 (= H50), P48 (≠ A52), L50 (= L54), V172 (= V178), A201 (= A211), G202 (= G212), W204 (= W214), H223 (= H233), Y257 (= Y258), G331 (= G324), I332 (≠ P325), V358 (≠ I354), W359 (≠ G355), V360 (≠ I356), T361 (≠ V357)
Q9AGP8 Dimethylglycine oxidase; DMGO; EC 1.5.3.10 from Arthrobacter globiformis (see 2 papers)
32% identity, 99% coverage: 10:814/815 of query aligns to 6:829/830 of Q9AGP8
- IV 14:15 (≠ VS 18:19) binding
- DQ 35:36 (≠ ER 39:40) binding
- STSH 45:48 (≠ TTWH 47:50) binding
- L52 (= L54) binding
- V174 (= V178) binding
- H225 (= H233) Important for catalytic activity; mutation to Q: Reduces catalytic efficiency 3-fold and substrate affinity 30-fold.
- Y259 (= Y258) Important for catalytic activity; binding ; mutation to F: Reduces catalytic efficiency 225-fold and substrate affinity 25-fold.
- VWVT 360:363 (≠ IGIV 354:357) binding
- Y539 (= Y524) binding
- D552 (= D537) Important for catalytic activity; mutation to A: No effect on the activity.; mutation to N: Reduces activity 3-fold.
3gsiA Crystal structure of d552a dimethylglycine oxidase mutant of arthrobacter globiformis in complex with tetrahydrofolate (see paper)
32% identity, 99% coverage: 10:814/815 of query aligns to 3:826/827 of 3gsiA
- active site: H222 (= H233), Y256 (= Y258), A549 (≠ D537)
- binding flavin-adenine dinucleotide: G10 (= G17), I11 (≠ V18), V12 (≠ S19), D32 (≠ E39), Q33 (≠ R40), G41 (= G46), S42 (≠ T47), T43 (= T48), H45 (= H50), P47 (≠ A52), L49 (= L54), T170 (≠ K177), V171 (= V178), A200 (= A211), G201 (= G212), W203 (= W214), H222 (= H233), Y256 (= Y258), G330 (= G324), I331 (≠ P325), F332 (≠ E326), V357 (≠ I354), W358 (≠ G355), V359 (≠ I356), T360 (≠ V357)
- binding magnesium ion: D254 (≠ C256), V409 (≠ G406)
- binding (6s)-5,6,7,8-tetrahydrofolate: L505 (≠ F493), Y536 (= Y524), T551 (= T539), G563 (= G554), F629 (≠ A612), Y648 (= Y631), E655 (= E638), Y696 (≠ F679)
1worA Crystal structure of t-protein of the glycine cleavage system (see paper)
28% identity, 47% coverage: 424:806/815 of query aligns to 1:356/362 of 1worA
1wopA Crystal structure of t-protein of the glycine cleavage system (see paper)
28% identity, 47% coverage: 424:806/815 of query aligns to 1:356/362 of 1wopA
- active site: D96 (= D537)
- binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: M51 (≠ F493), L55 (≠ R497), Y83 (= Y524), D96 (= D537), V98 (≠ T539), E106 (≠ A547), L108 (≠ F549), V110 (= V551), N112 (≠ P553), I137 (≠ V578), E160 (≠ P601), Y168 (≠ H609), Y169 (≠ F611), K173 (≠ R615), S174 (≠ E616), I175 (= I617), E180 (≠ G622), T181 (≠ A624), Y188 (= Y631), E195 (= E638), M197 (≠ Y640), R227 (≠ L670), Y236 (≠ F679)
Sites not aligning to the query:
1wooA Crystal structure of t-protein of the glycine cleavage system (see paper)
28% identity, 47% coverage: 424:806/815 of query aligns to 1:356/362 of 1wooA
- active site: D96 (= D537)
- binding (6s)-5,6,7,8-tetrahydrofolate: M51 (≠ F493), Y83 (= Y524), D96 (= D537), V98 (≠ T539), V110 (= V551), N112 (≠ P553), Y168 (≠ H609), Y169 (≠ F611), Y188 (= Y631), E195 (= E638), Y236 (≠ F679)
Sites not aligning to the query:
Q46337 Sarcosine oxidase subunit alpha; Sarcosine oxidase subunit A; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit alpha; Tetrameric sarcosine oxidase subunit alpha; TSOX subunit alpha; EC 1.5.3.24 from Corynebacterium sp. (strain P-1) (see 2 papers)
28% identity, 48% coverage: 425:812/815 of query aligns to 577:964/967 of Q46337
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 139 G→A: Does not affect activity and binding of NAD(+).
3a8iA Crystal structure of et-ehred-5-ch3-thf complex (see paper)
29% identity, 38% coverage: 463:772/815 of query aligns to 21:329/363 of 3a8iA
- active site: D97 (= D537)
- binding 5-methyl-5,6,7,8-tetrahydrofolic acid: M51 (≠ F493), Y84 (= Y524), D97 (= D537), I99 (≠ T539), V111 (= V551), N113 (≠ P553), F173 (= F611), Y188 (= Y631), E195 (= E638), R223 (≠ L670), M232 (≠ F679), W252 (≠ F699)
3ad7A Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with methylthio acetate (see paper)
29% identity, 36% coverage: 425:720/815 of query aligns to 574:863/963 of 3ad7A
Sites not aligning to the query:
- active site: 349, 374
- binding flavin mononucleotide: 509, 510, 516, 520, 548, 550
- binding nicotinamide-adenine-dinucleotide: 134, 136, 137, 138, 157, 158, 159, 165, 204, 248, 249, 294, 380, 416, 422, 423, 424
2gagA Heteroteterameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution (see paper)
28% identity, 48% coverage: 425:812/815 of query aligns to 575:962/965 of 2gagA
Sites not aligning to the query:
- active site: 350, 375
- binding flavin mononucleotide: 510, 511, 517, 521, 549, 551
- binding nicotinamide-adenine-dinucleotide: 134, 135, 137, 138, 139, 158, 159, 160, 165, 166, 204, 205, 249, 250, 295, 381, 417, 418, 423, 424, 425, 554
1vrqA Crystal structure of heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with folinic acid (see paper)
29% identity, 36% coverage: 425:720/815 of query aligns to 574:863/963 of 1vrqA
- active site: D676 (= D537)
- binding N-{[4-({[(6R)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]carbonyl}-L-glutamic acid: L631 (≠ F493), Y663 (= Y524), G677 (≠ L538), H690 (≠ V551), I774 (≠ V629), F776 (≠ Y631), E783 (= E638), K822 (= K677), F824 (= F679)
Sites not aligning to the query:
- active site: 349, 374
- binding flavin mononucleotide: 509, 510, 516, 520, 548, 550
- binding nicotinamide-adenine-dinucleotide: 134, 136, 137, 138, 157, 158, 159, 165, 204, 248, 249, 294, 380, 416, 422, 423, 424
Q50LF0 Sarcosine oxidase subunit alpha; Sarcosine oxidase subunit A; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit alpha; Tetrameric sarcosine oxidase subunit alpha; TSOX subunit alpha; EC 1.5.3.24 from Corynebacterium sp. (strain U-96) (see 2 papers)
29% identity, 36% coverage: 425:720/815 of query aligns to 575:864/965 of Q50LF0
Sites not aligning to the query:
- 139 binding
- 158 binding
- 159 binding
- 160 binding
- 166 binding
- 205 binding
- 418 binding
- 423 binding
- 425 binding
P48728 Aminomethyltransferase, mitochondrial; Glycine cleavage system T protein; GCVT; EC 2.1.2.10 from Homo sapiens (Human) (see 4 papers)
26% identity, 44% coverage: 416:775/815 of query aligns to 24:368/403 of P48728
- D129 (= D537) mutation D->A,N: Loss of aminomethyltransferase activity.
- N145 (≠ P553) to I: in GCE2; loss of aminomethyltransferase activity; dbSNP:rs386833682
- E232 (= E638) binding
- R261 (≠ L670) binding
- G269 (≠ A678) to D: in GCE2; decreased aminomethyltransferase activity; dbSNP:rs121964981
- R320 (≠ L726) to H: in GCE2; loss of aminomethyltransferase activity; dbSNP:rs121964985
Sites not aligning to the query:
1wsvA Crystal structure of human t-protein of glycine cleavage system (see paper)
26% identity, 43% coverage: 424:775/815 of query aligns to 2:337/371 of 1wsvA
- active site: D98 (= D537)
- binding n-[4-({[(6s)-2-amino-4-hydroxy-5-methyl-5,6,7,8-tetrahydropteridin-6-yl]methyl}amino)benzoyl]-l-glutamic acid: M53 (≠ F493), L85 (≠ Y524), D98 (= D537), L99 (= L538), I100 (≠ T539), V112 (= V551), N114 (≠ P553), F173 (= F611), G193 (≠ T630), Y194 (= Y631), E201 (= E638), R230 (≠ L670), L239 (≠ F679)
Sites not aligning to the query:
1y56B Crystal structure of l-proline dehydrogenase from p.Horikoshii (see paper)
26% identity, 45% coverage: 5:374/815 of query aligns to 1:355/374 of 1y56B
- active site: F44 (≠ W49), G47 (≠ A52), T48 (≠ G53), H224 (= H233), P239 (≠ L247), G305 (= G324), M338 (≠ V357)
- binding flavin-adenine dinucleotide: G11 (= G15), G13 (= G17), I14 (≠ V18), V15 (≠ S19), I33 (≠ L38), E34 (= E39), K35 (≠ R40), S42 (≠ T47), T43 (= T48), R45 (≠ H50), C46 (≠ A51), G47 (≠ A52), G49 (≠ L54), E170 (≠ K177), V171 (= V178), T200 (≠ A211), N201 (≠ G212), W203 (= W214), G305 (= G324), Y306 (≠ P325), Y307 (≠ E326), G334 (≠ S353), H335 (≠ I354), G336 (= G355), F337 (≠ I356), M338 (≠ V357)
- binding flavin mononucleotide: F44 (≠ W49), R45 (≠ H50), I260 (= I268), R301 (≠ T320), W303 (≠ F322)
Query Sequence
>SMc01662 FitnessBrowser__Smeli:SMc01662
MTKPIPAKARVVIIGGGVSGCSVAYHLAKLGWTDVVLLERKQLTCGTTWHAAGLIGQLRA
SQNMTRLAKYSADLYVKLEAETGIATGMRQNGSITVALTEERKEEIYRQASLARAFNVDV
REVTPDEVKELYPHLNVSDVKAAVHLPLDGQCDPANIAMALAKGARQNGATIVEGVKVTS
VLKKDGRITGVTCEQNGESFTIETENVVNCAGMWGRELARQSGVTVPLHACEHFYIVTEA
IPGLSRLPVLRVPDECTYYKEDAGKMLIGAFELKAKPWGMEGIREDFCFDQLPEDFDHFA
PILEMAVNRMPMLETAGIHTFFNGPESFTPDDRYYLGEAPELKGYWVAAGYNSIGIVSSG
GAGMALAQWMNDGEPPFDLWEVDIRRAQPFQKNRSYLKERVSETLGLLYADHFPYRQIAT
ARGVRRSPIHEQLKARGAVFGEVAGWERANWFARDGQEREYRYSWKRQNWFENQREEHLA
VRSGVGLFDMTSFGKIRVEGRDAQAFLQRLCANEMNVDPGRVVYTQMLNARGGIESDLTV
TRLSQTAFFLVVPGATLQRDLAWLRKHVRDEFVVITDVTAAESVLCVMGPRARELMQKVS
PNDFSNEAHPFATAREIEIGMGLARAHRVTYVGELGWELYVSTDQAAHVFETLELAGADV
GLKLCGLHTLDSCRIEKAFRHFGHDITDEDHVLEAGLGFAVKPGKGEFIGREAVLAKRDN
GLSRRLVQFRLSDPEPLLFHNEAIVRDGEIVGTITSGNYGHHLGGAIGLGYVACKGESDA
DVLASAYEIEIAGTRVKAEASLKPMYDPKAERVRA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory