SitesBLAST
Comparing SMc02117 FitnessBrowser__Smeli:SMc02117 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8sadA Crystal structure of cystathionine beta lyase from klebsiella aerogenes, plp/malonate complex (c2 form)
47% identity, 97% coverage: 9:390/395 of query aligns to 5:395/398 of 8sadA
- binding magnesium ion: A359 (≠ N353), R362 (= R357), A365 (= A360)
- binding pyridoxal-5'-phosphate: C88 (≠ S87), G89 (= G88), A90 (≠ L89), Y114 (= Y113), D188 (= D185), A210 (= A207), T212 (= T209), K213 (= K210), M222 (≠ L219), W343 (= W342)
8sabA Crystal structure of cystathionine beta lyase from klebsiella aerogenes, plp adduct with alanine (c2 form)
47% identity, 96% coverage: 13:390/395 of query aligns to 3:389/392 of 8sabA
- binding lysine: N9 (≠ H19), R12 (≠ N22), R13 (≠ N23), K14 (≠ P24), T17 (≠ F27), L330 (= L335), E341 (= E346)
- binding pyridoxal-5'-phosphate: C82 (≠ S87), G83 (= G88), A84 (≠ L89), Y108 (= Y113), D182 (= D185), A204 (= A207), T206 (= T209), K207 (= K210), M216 (≠ L219), W337 (= W342)
- binding alanyl-pyridoxal-5'-phosphate: C82 (≠ S87), G83 (= G88), A84 (≠ L89), Y108 (= Y113), D182 (= D185), A204 (= A207), T206 (= T209), K207 (= K210), M216 (≠ L219), Y335 (= Y340), S336 (= S341), W337 (= W342), R369 (= R370)
8u99A Crystal structure of cystathionine beta lyase from klebsiella aerogenes (plp-serine adduct)
47% identity, 96% coverage: 13:390/395 of query aligns to 2:388/391 of 8u99A
- binding pyridoxal-5'-phosphate: C81 (≠ S87), G82 (= G88), A83 (≠ L89), Y107 (= Y113), D181 (= D185), T205 (= T209), K206 (= K210), M215 (≠ L219), W336 (= W342)
- binding serine: Y107 (= Y113), K206 (= K210), Y334 (= Y340), S335 (= S341), W336 (= W342), R368 (= R370)
8u98A Crystal structure of cystathionine beta lyase from klebsiella aerogenes (plp-glycine adduct)
47% identity, 96% coverage: 13:390/395 of query aligns to 2:388/391 of 8u98A
- binding glycine: Y107 (= Y113), K206 (= K210), Y334 (= Y340), S335 (= S341), W336 (= W342), R368 (= R370)
- binding pyridoxal-5'-phosphate: Y52 (= Y58), R54 (≠ T60), C81 (≠ S87), G82 (= G88), A83 (≠ L89), Y107 (= Y113), D181 (= D185), A203 (= A207), T205 (= T209), K206 (= K210), M215 (≠ L219), W336 (= W342)
8sa9A Crystal structure of cystathionine beta lyase from klebsiella aerogenes, plp-oxamate adduct (c2 form)
47% identity, 96% coverage: 13:390/395 of query aligns to 2:388/391 of 8sa9A
- binding pyridoxal-5'-phosphate: C81 (≠ S87), G82 (= G88), A83 (≠ L89), Y107 (= Y113), D181 (= D185), A203 (= A207), T205 (= T209), K206 (= K210), M215 (≠ L219), W336 (= W342)
- binding [({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino](oxo)acetic acid: C81 (≠ S87), G82 (= G88), A83 (≠ L89), Y107 (= Y113), D181 (= D185), A203 (= A207), T205 (= T209), K206 (= K210), M215 (≠ L219), S335 (= S341), W336 (= W342), R368 (= R370)
P06721 Cystathionine beta-lyase MetC; CBL; CL; Beta-cystathionase MetC; Cysteine desulfhydrase MetC; CD; Cysteine lyase MetC; Cysteine-S-conjugate beta-lyase MetC; EC 4.4.1.13; EC 4.4.1.28 from Escherichia coli (strain K12) (see 2 papers)
46% identity, 97% coverage: 9:390/395 of query aligns to 2:392/395 of P06721
- K210 (= K210) modified: N6-(pyridoxal phosphate)lysine
1cl1B Cystathionine beta-lyase (cbl) from escherichia coli (see paper)
46% identity, 96% coverage: 11:390/395 of query aligns to 1:389/392 of 1cl1B
2gqnA Cystathionine beta-lyase (cbl) from escherichia coli in complex with n-hydrazinocarbonylmethyl-2-nitro-benzamide (see paper)
46% identity, 96% coverage: 13:390/395 of query aligns to 2:388/391 of 2gqnA
- active site: R54 (≠ T60), Y107 (= Y113), D181 (= D185), K206 (= K210)
- binding (5-hydroxy-6-methyl-4-((2-(2-(2-nitrobenzamido)acetyl)hydrazinyl)methyl)pyridin-3-yl)methyl dihydrogen phosphate: C81 (≠ S87), G82 (= G88), A83 (≠ L89), Y107 (= Y113), E108 (≠ F114), D181 (= D185), A203 (= A207), T205 (= T209), K206 (= K210), M215 (≠ L219), Y334 (= Y340), S335 (= S341), W336 (= W342), R368 (= R370)
2fq6A Cystathionine beta-lyase (cbl) from escherichia coli in complex with n-hydrazinocarbonylmethyl-2-trifluoromethyl-benzamide (see paper)
46% identity, 96% coverage: 13:390/395 of query aligns to 2:388/391 of 2fq6A
- active site: R54 (≠ T60), Y107 (= Y113), D181 (= D185), K206 (= K210)
- binding phosphoric acid mono-(5-hydroxy-6-methyl-4-{[2-(2-trifluoromethyl-benzoylamino)-acetyl]-hydrazonomethyl}-pyridin-3-ylmethyl)ester: C81 (≠ S87), G82 (= G88), A83 (≠ L89), Y107 (= Y113), P109 (= P115), D181 (= D185), A203 (= A207), T205 (= T209), K206 (= K210), M215 (≠ L219), Y334 (= Y340), S335 (= S341), W336 (= W342), R368 (= R370)
1cl2A Cystathionine beta-lyase (cbl) from escherichia coli in complex with aminoethoxyvinylglycine (see paper)
46% identity, 96% coverage: 13:390/395 of query aligns to 2:388/391 of 1cl2A
- active site: R54 (≠ T60), Y107 (= Y113), D181 (= D185), K206 (= K210)
- binding (2E,3E)-4-(2-aminoethoxy)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: C81 (≠ S87), G82 (= G88), A83 (≠ L89), Y107 (= Y113), D181 (= D185), A203 (= A207), T205 (= T209), K206 (= K210), M215 (≠ L219), Y334 (= Y340), S335 (= S341), W336 (= W342), R368 (= R370)
4itxA P113s mutant of e. Coli cystathionine beta-lyase metc inhibited by reaction with l-ala-p (see paper)
46% identity, 96% coverage: 13:390/395 of query aligns to 2:388/391 of 4itxA
- active site: R54 (≠ T60), Y107 (= Y113), D181 (= D185), K206 (= K210)
- binding {1-[(3-hydroxy-methyl-5-phosphonooxy-methyl-pyridin-4-ylmethyl)-amino]-ethyl}-phosphonic acid: C81 (≠ S87), G82 (= G88), A83 (≠ L89), Y107 (= Y113), D181 (= D185), A203 (= A207), T205 (= T209), K206 (= K210), M215 (≠ L219), Y334 (= Y340), S335 (= S341), W336 (= W342), R368 (= R370)
4l0oH Structure determination of cystathionine gamma-synthase from helicobacter pylori
35% identity, 96% coverage: 13:390/395 of query aligns to 3:371/373 of 4l0oH
- active site: R40 (≠ T60), Y92 (= Y113), D164 (= D185), K189 (= K210)
- binding pyridoxal-5'-phosphate: Y38 (= Y58), R40 (≠ T60), S67 (= S87), G68 (= G88), L69 (= L89), Y92 (= Y113), D164 (= D185), S186 (≠ A207), T188 (= T209), K189 (= K210)
8j6nA Crystal structure of cystathionine gamma-lyase in complex with compound 1 (see paper)
34% identity, 97% coverage: 11:393/395 of query aligns to 7:389/390 of 8j6nA
- binding [6-methyl-4-[(~{E})-(oxamoylhydrazinylidene)methyl]-5-oxidanyl-pyridin-3-yl]methyl dihydrogen phosphate: Y51 (= Y58), R53 (≠ T60), G81 (= G88), L82 (= L89), Y105 (= Y113), E148 (= E156), N152 (≠ S160), D178 (= D185), S200 (≠ A207), T202 (= T209), K203 (= K210), E330 (≠ Y340), S331 (= S341), T346 (≠ V350), R366 (= R370)
4hf8A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with glycine (see paper)
32% identity, 97% coverage: 12:393/395 of query aligns to 7:396/396 of 4hf8A
- active site: R59 (≠ T60), Y112 (= Y113), D184 (= D185), K209 (= K210)
- binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: G87 (= G88), I88 (≠ L89), Y112 (= Y113), E155 (= E156), N159 (≠ S160), D184 (= D185), S206 (≠ A207), K209 (= K210), S338 (= S341), R373 (= R370)
4omaA The crystal structure of methionine gamma-lyase from citrobacter freundii in complex with l-cycloserine pyridoxal-5'-phosphate (see paper)
32% identity, 97% coverage: 12:393/395 of query aligns to 7:396/396 of 4omaA
- active site: R59 (≠ T60), Y112 (= Y113), D184 (= D185), K209 (= K210)
- binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: G87 (= G88), I88 (≠ L89), Y112 (= Y113), D184 (= D185), S206 (≠ A207), T208 (= T209), K209 (= K210), V337 (≠ Y340), S338 (= S341), R373 (= R370)
3jwbA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with norleucine (see paper)
32% identity, 97% coverage: 12:393/395 of query aligns to 7:396/396 of 3jwbA
3jwaA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with methionine phosphinate (see paper)
32% identity, 97% coverage: 12:393/395 of query aligns to 7:396/396 of 3jwaA
3jw9A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with s-ethyl-cysteine (see paper)
32% identity, 97% coverage: 12:393/395 of query aligns to 7:396/396 of 3jw9A
5m3zA Crystal structure of citrobacter freundii methionine gamma-lyase with c115h replacement in the complex with l-norleucine (see paper)
32% identity, 97% coverage: 12:393/395 of query aligns to 6:395/395 of 5m3zA
- active site: R58 (≠ T60), Y111 (= Y113), D183 (= D185), K208 (= K210)
- binding norleucine: Y111 (= Y113), H113 (≠ P115), K208 (= K210), V336 (≠ Y340), S337 (= S341)
- binding pyridoxal-5'-phosphate: G86 (= G88), I87 (≠ L89), Y111 (= Y113), E154 (= E156), D183 (= D185), T185 (= T187), S205 (≠ A207), T207 (= T209), K208 (= K210)
- binding 2-[o-phosphonopyridoxyl]-amino-hexanoic acid: G86 (= G88), I87 (≠ L89), Y111 (= Y113), D183 (= D185), S205 (≠ A207), T207 (= T209), K208 (= K210), V336 (≠ Y340), S337 (= S341), R372 (= R370)
6egrA Crystal structure of citrobacter freundii methionine gamma-lyase with v358y replacement (see paper)
31% identity, 97% coverage: 12:393/395 of query aligns to 7:396/396 of 6egrA
Query Sequence
>SMc02117 FitnessBrowser__Smeli:SMc02117
MADNKIGKAEQGINTRLAHTGNNPSDFHGFVNPPVVHASTVLFPNARTMETRAQKYTYGT
RGTPTTDALCEAVNELEGAAGTILVPSGLAAVTVPFLAYLSSGDHVLIVDSVYFPTRHFC
DTMLSRLGVTVEYYDPTIGAGIENLIRPNTRLVHTEAPGSNTFEMQDIPAIAAAAHRHGC
IVTMDNTWATPVYFRPLDHGVDVSIHAATKYPSGHSDVLLGTVSANAAHWPALSEAMVTL
GVSVSPDDSYQILRGLRTMGVRLVRHQASALAIAEWLEGRDEVARVLHPALPSFPGHELW
KRDFGGASGIFSFVLKAEPEDGKAKAHAFLDALSLFGLGYSWGGFESLAVHVNLSDRKVA
KAPSEGPVIRLQIGLEDVPDIRRDIEAGLAAANAV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory