SitesBLAST
Comparing SMc02169 FitnessBrowser__Smeli:SMc02169 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P04983 Ribose import ATP-binding protein RbsA; EC 7.5.2.7 from Escherichia coli (strain K12) (see paper)
36% identity, 95% coverage: 4:251/260 of query aligns to 2:241/501 of P04983
- K43 (= K45) mutation to R: Loss of transport.
4u00A Crystal structure of ttha1159 in complex with adp (see paper)
31% identity, 83% coverage: 5:221/260 of query aligns to 1:206/241 of 4u00A
O95477 Phospholipid-transporting ATPase ABCA1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1; ABC-1; ATP-binding cassette 1; Cholesterol efflux regulatory protein; EC 7.6.2.1 from Homo sapiens (Human) (see 35 papers)
31% identity, 89% coverage: 1:232/260 of query aligns to 888:1112/2261 of O95477
- D917 (= D23) to Y: in a colorectal cancer sample; somatic mutation
- T929 (≠ L35) to I: in TGD; moderately decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; loss protein subcellular localization to the plasma membrane
- N935 (= N41) to S: in TGD; moderately decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; dbSNP:rs28937313
- K939 (= K45) mutation to M: Inhibits ATPase activity; when associated with M-1952. Decreases translocase activity; when associated with M-1952. Does not affect protein subcellular localization in plasma membrane and endosome; when associated with M-1952.
- S1042 (≠ A161) modified: Phosphoserine; by PKA
- P1065 (≠ V184) natural variant: P -> S
- M1091 (= M211) to T: in FHA1; loss of localization to plasma membrane; decreased cholesterol efflux; decreased phospholipid efflux
- C1110 (≠ L230) modified: S-palmitoyl cysteine; mutation to S: Decreased palmitoylation; when associated with S-3, S-23 and S-1111.
- C1111 (= C231) modified: S-palmitoyl cysteine; mutation to S: Decreased palmitoylation; when associated with S-3, S-23 and S-1110.
Sites not aligning to the query:
- 3 modified: S-palmitoyl cysteine; C→S: Mild decrease of palmitoylation. Loss of localization to plasma membrane. Decreased cholesterol efflux. Decreased phospholipid efflux. Decreased palmitoylation; when associated with S-23, S-1110 and S-1111.
- 23 modified: S-palmitoyl cysteine; C→S: Mild decrease of palmitoylation. Loss of localization to plasma membrane. Decreased palmitoylation; when associated with S-3, S-1110 and S-1111.
- 74 I→C: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with C-371.; I→K: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with E-371.
- 75 modified: Disulfide link with 309
- 85 P → L: in FHA1; Alabama; dbSNP:rs145183203
- 98 modified: carbohydrate, N-linked (GlcNAc...) asparagine
- 100 S→C: Highly decreased protein abundance. Highly decreased ATPase activity. Highly decreased phospholipid translocase activity.
- 210 E → D: in a colorectal cancer sample; somatic mutation
- 219 R → K: associated with a decreased severity of CAD; dbSNP:rs2230806
- 230 R → C: in dbSNP:rs9282541
- 244 modified: carbohydrate, N-linked (GlcNAc...) asparagine
- 248 P → A: in dbSNP:rs142625938
- 255 A → T: in TGD; deficient cellular cholesterol efflux; dbSNP:rs758100110
- 304 V→C: No effect on phospholipid and cholesterol efflux or localization to cell membrane; when associated with C-308.
- 308 V→C: No effect on phospholipid and cholesterol efflux or localization to cell membrane; when associated with C-304.
- 309 modified: Disulfide link with 75
- 364 S → C: in dbSNP:rs775035559
- 371 I→C: No effect on phospholipid and cholesterol efflux or localization to cell membrane. 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with C-74 or C-375.; I→E: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-74.
- 375 L→C: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with C-371.
- 399 V → A: in dbSNP:rs9282543
- 401 K → Q: in dbSNP:rs138487227
- 496 R → W: associated with increased plasma HDL cholesterol; dbSNP:rs147675550
- 568 K→A: 60-65% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane.
- 573 Y→F: No effect on phospholipid and cholesterol efflux and on localization to cell membrane.
- 581 D→K: 80-85% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-584 and K-585.
- 583 F→K: 90-95% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with E-590.
- 584 E→K: 80-85% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-581 and K-585.
- 585 D→K: 80-85% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-581 and K-584.
- 590 W → S: in TGD; moderately decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; dbSNP:rs137854496; W→E: 90-95% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-583.
- 593 F→L: Moderately decreased protein abundance. Highly decreased ATPase activity. Highly decreased phospholipid translocase activity.
- 638 R → Q: associated with reduced plasma HDL cholesterol; dbSNP:rs374190304
- 771 V → M: associated with HDL cholesterol; dbSNP:rs2066718
- 774 T → P: in dbSNP:rs35819696; natural variant: T -> S
- 776 K → N: may be associated with increased risk of ischemic heart disease; dbSNP:rs138880920
- 815 E → G: associated with reduced plasma HDL cholesterol; dbSNP:rs145582736
- 825 V → I: associated with higher plasma cholesterol; dbSNP:rs2066715
- 883 I → M: associated with higher plasma cholesterol; dbSNP:rs2066714
- 1172 E → D: associated with premature coronary heart disease; dbSNP:rs33918808
- 1181 S → F: associated with reduced plasma HDL cholesterol; dbSNP:rs76881554
- 1216 G → V: in dbSNP:rs562403512
- 1341 R → T: associated with reduced plasma HDL cholesterol; dbSNP:rs147743782
- 1376 S → G: in dbSNP:rs145689805
- 1379 L → F: in TGD; the mutant protein is retained in the endoplasmic reticulum while the wild-type protein is located at the plasma membrane
- 1407 A → T: in a colorectal cancer sample; somatic mutation; dbSNP:rs189206655
- 1463 modified: Disulfide link with 1477
- 1477 modified: Disulfide link with 1463; C → R: in TGD; loss of interaction with APOE; unable to generate APOE-containing high density lipoproteins; moderately decreased protein abundance; moderately decreased ATPase activity; moderately decreased phospholipid translocase activity; dbSNP:rs137854494
- 1512 T→M: Moderately decreased protein abundance. Does not affect ATPase activity. Moderately decreased phospholipid translocase activity.
- 1555 I → T: in dbSNP:rs1997618
- 1587 K → R: associated with HDL cholesterol; dbSNP:rs2230808
- 1611 N → D: probable disease-associated variant; associated with atherosclerosis; deficient cellular cholesterol efflux
- 1615 R → Q: associated with reduced plasma HDL cholesterol; dbSNP:rs1251839800
- 1648 L → P: in dbSNP:rs1883024
- 1670 A → T: associated with reduced plasma HDL cholesterol; dbSNP:rs1203589782
- 1680 R → Q: associated with increased plasma HDL cholesterol; dbSNP:rs150125857
- 1704 V → D: in TGD; the mutant protein is retained in the endoplasmic reticulum while the wild-type protein is located at the plasma membrane
- 1731 S → C: in dbSNP:rs760507032
- 1897 R → W: in FHA1; uncertain pathological significance; dbSNP:rs760768125
- 1925 R → Q: in Scott syndrome; shows impaired trafficking of the mutant protein to the plasma membrane; dbSNP:rs142688906
- 1952 K→M: Inhibits ATPase activity; when associated with M-939. Decreases translocase activity; when associated with M-939. Does not affect protein subcellular localization in plasma membrane and endosome; when associated with M-939.
- 2054 modified: Phosphoserine; by PKA
- 2081 R → W: in TGD; highly decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; loss protein subcellular localization to the plasma membrane; dbSNP:rs137854501
- 2109 A → T: in a colorectal cancer sample; somatic mutation
- 2150 P → L: in FHA1; moderately decreased protein abundance; does not affect ATPase activity; moderately decreased phospholipid translocase activity; dbSNP:rs369098049
- 2163 F → S: could be associated with reduced plasma HDL cholesterol
- 2168 L → P: in dbSNP:rs2853577
- 2243 D → E: in dbSNP:rs34879708
- 2244 V → I: could be associated with reduced plasma HDL cholesterol; dbSNP:rs144588452
P41233 Phospholipid-transporting ATPase ABCA1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1; ABC-1; ATP-binding cassette 1; EC 7.6.2.1 from Mus musculus (Mouse) (see paper)
31% identity, 89% coverage: 1:232/260 of query aligns to 888:1112/2261 of P41233
Sites not aligning to the query:
- 489 modified: carbohydrate, N-linked (GlcNAc...) asparagine
7tbwA The structure of atp-bound abca1 (see paper)
31% identity, 87% coverage: 7:232/260 of query aligns to 754:967/1928 of 7tbwA
- binding adenosine-5'-triphosphate: Y763 (= Y16), D765 (vs. gap), K768 (≠ V19), A770 (= A21), H789 (≠ D40), N790 (= N41), G791 (= G42), G793 (= G44), K794 (= K45), T795 (≠ S46), T796 (≠ S47), Q835 (≠ A95), K880 (≠ N144), Q887 (≠ T151), S889 (= S153), G891 (= G155), Q913 (≠ E177), H944 (= H209)
- binding magnesium ion: Q835 (≠ A95), D912 (= D176), Q913 (≠ E177)
Sites not aligning to the query:
- binding adenosine-5'-triphosphate: 1630, 1633, 1657, 1658, 1660, 1661, 1662, 1663, 1702, 1753, 1755, 1756, 1757, 1779, 1783, 1811
- binding cholesterol: 213, 218, 219, 352, 360, 367
- binding magnesium ion: 1662, 1702, 1779
1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
28% identity, 84% coverage: 6:223/260 of query aligns to 4:222/253 of 1g9xB
1g6hA Crystal structure of the adp conformation of mj1267, an atp- binding cassette of an abc transporter (see paper)
25% identity, 84% coverage: 6:223/260 of query aligns to 4:222/254 of 1g6hA
7tbyA The structure of human abca1 in nanodisc (see paper)
30% identity, 89% coverage: 1:232/260 of query aligns to 680:894/1788 of 7tbyA
Sites not aligning to the query:
7roqA Alternative structure of human abca1
30% identity, 89% coverage: 1:232/260 of query aligns to 768:982/1831 of 7roqA
Sites not aligning to the query:
F1MWM0 Retinal-specific phospholipid-transporting ATPase ABCA4; ATP-binding cassette sub-family A member 4; RIM ABC transporter; RIM protein; RmP; Retinal-specific ATP-binding cassette transporter; EC 7.6.2.1 from Bos taurus (Bovine) (see 2 papers)
30% identity, 89% coverage: 5:235/260 of query aligns to 927:1144/2281 of F1MWM0
Sites not aligning to the query:
- 415 modified: carbohydrate, N-linked (Hex...) asparagine
- 504 modified: carbohydrate, N-linked (Hex...) asparagine
- 901 modified: Phosphothreonine; T→A: Decreases expression level. Affects subcellular location.
- 1185 modified: Phosphoserine; S→A: Does not affect subcellular location. Does not affect expression level. Does not affect ATPase activity. Reduces the stimulating effect of all-trans-retinal on ATP hydrolysis.
- 1309 Cleavage; by trypsin
- 1313 modified: Phosphothreonine; T→A: Does not affect subcellular location. Does not affect expression level. Does not affect ATPase activity. Reduces the stimulating effect of all-trans-retinal on ATP hydrolysis.
- 1317 modified: Phosphoserine; S→A: Does not affect subcellular location. Does not affect expression level. Affects both the basal and stimulated ATPase activity.
- 1319 modified: Phosphoserine
- 1455 modified: carbohydrate, N-linked (Hex...) asparagine
- 1527 modified: carbohydrate, N-linked (Hex...) asparagine
- 1660 modified: carbohydrate, N-linked (Hex...) asparagine
5x40A Structure of a cbio dimer bound with amppcp (see paper)
29% identity, 96% coverage: 5:254/260 of query aligns to 3:242/280 of 5x40A
- binding phosphomethylphosphonic acid adenylate ester: F14 (≠ Y16), V18 (= V19), K19 (≠ T20), A20 (= A21), P39 (≠ D40), N40 (= N41), G41 (= G42), A42 (= A43), G43 (= G44), K44 (= K45), S45 (= S46), T46 (≠ S47), Q88 (= Q88), R136 (≠ Q147), H139 (≠ E150), M140 (≠ T151), L141 (= L152), S142 (= S153), G143 (= G154), G144 (= G155), Q145 (= Q156), Q166 (≠ E177), H198 (= H209)
- binding magnesium ion: S45 (= S46), Q88 (= Q88)
3c4jA Abc protein artp in complex with atp-gamma-s
29% identity, 83% coverage: 6:221/260 of query aligns to 3:208/242 of 3c4jA
- binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y16), V18 (≠ A21), P37 (≠ D40), S38 (≠ N41), G39 (= G42), S40 (≠ A43), G41 (= G44), K42 (= K45), S43 (= S46), T44 (≠ S47), H133 (≠ N146), Y135 (≠ A148), E164 (= E177)
3c41J Abc protein artp in complex with amp-pnp/mg2+
29% identity, 83% coverage: 6:221/260 of query aligns to 3:208/242 of 3c41J
- binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y16), V18 (≠ A21), P37 (≠ D40), S38 (≠ N41), G39 (= G42), S40 (≠ A43), G41 (= G44), K42 (= K45), S43 (= S46), T44 (≠ S47), R47 (≠ K50), H133 (≠ N146), Y135 (≠ A148)
- binding magnesium ion: S43 (= S46), D163 (= D176)
2olkA Abc protein artp in complex with adp-beta-s
29% identity, 83% coverage: 6:221/260 of query aligns to 3:208/242 of 2olkA
2oljA Abc protein artp in complex with adp/mg2+
29% identity, 83% coverage: 6:221/260 of query aligns to 3:208/242 of 2oljA
- binding adenosine-5'-diphosphate: F13 (≠ Y16), V18 (≠ A21), S38 (≠ N41), G39 (= G42), S40 (≠ A43), G41 (= G44), K42 (= K45), S43 (= S46), T44 (≠ S47), H133 (≠ N146), Y135 (≠ A148)
P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
32% identity, 79% coverage: 19:223/260 of query aligns to 18:213/343 of P30750
- 40:46 (vs. 41:47, 71% identical) binding
- E166 (= E177) mutation to Q: Exhibits little ATPase activity.
Sites not aligning to the query:
- 278:283 binding
- 295 N→A: Reduces the binding of L-methionine to undetectable levels.
- 295:296 binding
2d62A Crystal structure of multiple sugar binding transport atp- binding protein
28% identity, 91% coverage: 14:249/260 of query aligns to 14:241/375 of 2d62A
3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
31% identity, 78% coverage: 19:221/260 of query aligns to 19:212/344 of 3tuzC
Sites not aligning to the query:
3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
31% identity, 78% coverage: 19:221/260 of query aligns to 19:212/344 of 3tuiC
Sites not aligning to the query:
6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
31% identity, 78% coverage: 19:221/260 of query aligns to 19:212/344 of 6cvlD