SitesBLAST
Comparing SMc02227 FitnessBrowser__Smeli:SMc02227 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8oqlA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-1
43% identity, 96% coverage: 1:709/737 of query aligns to 9:722/728 of 8oqlA
- binding Hexafluorophosphate anion: V37 (= V29), N39 (≠ T31), G75 (= G66), D77 (= D68), M81 (= M75), V92 (≠ L94), T95 (≠ L97), P148 (= P150), E149 (= E151), L152 (≠ V154), Q180 (≠ T181), Q260 (≠ E254), K362 (≠ I355), D363 (= D356), V364 (≠ R357), V430 (= V423), D476 (≠ E469), K477 (= K470), M478 (= M471), P479 (≠ M472), K506 (= K499)
- binding formamide: V78 (≠ I72), K79 (= K73)
4b3iA Crystal structure of mycobacterium tuberculosis fatty acid beta-oxidation complex with coenzymea bound at the hydratase active sites (see paper)
43% identity, 96% coverage: 1:709/737 of query aligns to 12:725/731 of 4b3iA
- active site: G79 (≠ A67), E100 (≠ G99), R104 (≠ G103), G127 (= G126), E130 (= E129), P151 (= P150), E152 (= E151), G160 (= G159), S452 (= S442), H473 (= H463), E485 (= E475), S523 (≠ N513)
- binding adenosine-5'-diphosphate: Q640 (≠ E630), P641 (≠ I631), P642 (≠ E632), L643 (≠ V633), Q644 (≠ G634)
- binding coenzyme a: T38 (≠ M27), V40 (= V29), A77 (≠ G65), G79 (≠ A67), D80 (= D68), V81 (≠ I72), E152 (= E151), F315 (= F305), Q319 (= Q309)
8oqsB Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-83
43% identity, 96% coverage: 1:709/737 of query aligns to 16:729/735 of 8oqsB
- binding 4-phenylbenzenesulfonic acid: M16 (= M1), P17 (≠ S2), M45 (≠ F30), I50 (≠ M35), G82 (= G66), G83 (≠ A67), D84 (= D68), T87 (≠ S74), T87 (≠ S74), M88 (= M75), M88 (= M75), A91 (≠ F78), D95 (≠ E82), D98 (≠ K93), V99 (≠ L94), T102 (≠ L97), T102 (≠ L97), E134 (= E129), E156 (= E151), L159 (≠ V154), F302 (= F288), F302 (= F288), F319 (= F305), S456 (= S442), T457 (= T443), M485 (= M471), P486 (≠ M472), G523 (= G509), S527 (≠ N513), N535 (≠ H521), P560 (= P546), M575 (≠ S561), I578 (= I564), I578 (= I564), I682 (= I668), M683 (≠ L669), G686 (= G672)
Sites not aligning to the query:
8oqvA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-109
43% identity, 96% coverage: 1:709/737 of query aligns to 8:721/726 of 8oqvA
- binding 4-nitrobenzenesulfonic acid: E39 (= E32), G74 (= G66), M80 (= M75), V91 (≠ L94), T94 (≠ L97), T94 (≠ L97), E148 (= E151), L151 (≠ V154), R182 (≠ S184), S448 (= S442), S519 (≠ N513), R520 (= R514), L562 (≠ V556), L566 (= L560), I570 (= I564)
Sites not aligning to the query:
8oqrA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-80
43% identity, 96% coverage: 1:709/737 of query aligns to 10:723/728 of 8oqrA
- binding 4-cyanobenzenesulfonic acid: G76 (= G66), G77 (≠ A67), T81 (≠ S74), M82 (= M75), M82 (= M75), A85 (≠ F78), D89 (≠ E82), T96 (≠ L97), L123 (≠ M124), G124 (= G125), P149 (= P150), E150 (= E151), S366 (≠ D358), L367 (≠ M359), E368 (= E360), A420 (= A412), V421 (= V413), F422 (= F414)
8oqoA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-49
43% identity, 96% coverage: 1:709/737 of query aligns to 9:722/727 of 8oqoA
8pf8A Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-72
43% identity, 96% coverage: 1:709/737 of query aligns to 10:723/729 of 8pf8A
- binding [2-methyl-5-(trifluoromethyl)pyrazol-3-yl]boronic acid: G76 (= G66), F169 (≠ T170), N173 (≠ D174), S177 (≠ Q177), I193 (≠ M193), F313 (= F305)
- binding bis[2-methyl-5-(trifluoromethyl)pyrazol-3-yl]-bis(oxidanyl)boranuide: M39 (≠ F30), N40 (≠ T31), E41 (= E32), T81 (≠ S74), D92 (≠ K93), V93 (≠ L94)
- binding [(2~{R})-2,3-bis(oxidanyl)propoxy]-[2-methyl-5-(trifluoromethyl)pyrazol-3-yl]borinic acid: M39 (≠ F30), G77 (≠ A67), D78 (= D68), M82 (= M75), V93 (≠ L94)
- binding (2~{R})-3-bis[2-methyl-5-(trifluoromethyl)pyrazol-3-yl]boranyloxypropane-1,2-diol: T152 (≠ K153), R184 (≠ S184), A311 (≠ S303), F312 (≠ L304), I673 (≠ V665)
Sites not aligning to the query:
8oquA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-92
43% identity, 96% coverage: 1:709/737 of query aligns to 11:724/730 of 8oquA
- binding 4-chloranylbenzenesulfonic acid: M40 (≠ F30), N41 (≠ T31), E42 (= E32), G77 (= G66), G78 (≠ A67), D79 (= D68), V80 (≠ I72), D90 (≠ E82), V94 (≠ L94), L124 (≠ M124), G125 (= G125), P150 (= P150), E151 (= E151)
8oqtA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-91
43% identity, 96% coverage: 1:709/737 of query aligns to 10:723/729 of 8oqtA
- binding 4-bromanylbenzenesulfonic acid: E41 (= E32), G76 (= G66), G77 (≠ A67), D78 (= D68), V79 (≠ I72), M82 (= M75), D89 (≠ E82), V93 (≠ L94), T96 (≠ L97), T96 (≠ L97), P149 (= P150), E150 (= E151)
Sites not aligning to the query:
8oqnA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-53
43% identity, 96% coverage: 1:709/737 of query aligns to 10:723/729 of 8oqnA
- binding 1-benzyl-1H-pyrazole-4-carboxylic acid: M39 (≠ F30), M82 (= M75), E150 (= E151), Q172 (= Q173), F175 (vs. gap), V176 (≠ L176), Q181 (≠ T181), T241 (≠ G234), F254 (≠ A247), N257 (≠ I250), Q261 (≠ E254), L262 (≠ T255), P266 (vs. gap), P268 (= P260), Q282 (= Q274), V283 (= V275), G302 (≠ T294), Q303 (≠ T295), V304 (≠ E296), S521 (≠ N513), G525 (≠ F517)
8opvA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with resveratrol (fragment-b-h11)
43% identity, 96% coverage: 1:709/737 of query aligns to 10:723/729 of 8opvA
- binding resveratrol: M39 (≠ F30), A75 (≠ G65), G76 (= G66), M82 (= M75), E128 (= E129), P149 (= P150), E150 (= E151), T152 (≠ K153), L153 (≠ V154), R184 (≠ S184), F296 (= F288)
8opuA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with sulfamethoxazole (fragment-b-e1)
43% identity, 96% coverage: 1:709/737 of query aligns to 10:723/729 of 8opuA
8oqqA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-79
43% identity, 96% coverage: 4:709/737 of query aligns to 7:717/723 of 8oqqA
8oqpA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-76
43% identity, 96% coverage: 4:709/737 of query aligns to 7:717/723 of 8oqpA
- binding 2-azanyl-5-sulfo-benzoic acid: G28 (≠ K25), S29 (= S26), A63 (≠ G59), K64 (= K60), K64 (= K60), K65 (≠ S61), P143 (= P150), E144 (= E151), L147 (≠ V154), F307 (= F305), M473 (= M471), P548 (= P546), S599 (≠ R595), L602 (≠ K598), K603 (≠ N599), S661 (≠ D659), T662 (≠ P660), G674 (= G672), A698 (≠ K690), R705 (≠ E697)
7o4uA Structure of the alpha subunit of mycobacterium tuberculosis beta- oxidation trifunctional enzyme in complex with oxidized nicotinamide adenine dinucleotide (see paper)
43% identity, 96% coverage: 4:709/737 of query aligns to 2:705/711 of 7o4uA
6yswA E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
35% identity, 98% coverage: 6:724/737 of query aligns to 4:703/707 of 6yswA
- active site: A66 (= A67), I71 (= I72), A84 (= A91), Q88 (≠ F95), G112 (= G126), E115 (= E129), P136 (= P150), E137 (= E151), G145 (= G159), D264 (≠ K282), S422 (= S442), H443 (= H463), E455 (= E475), N493 (= N513)
- binding coenzyme a: E23 (≠ K25), M25 (= M27), A66 (= A67), D67 (= D68), I68 (≠ L69), P136 (= P150), E137 (= E151), L140 (≠ V154), T290 (≠ Q309), K293 (≠ G312)
P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
33% identity, 98% coverage: 2:724/737 of query aligns to 39:762/763 of P40939
- V282 (≠ L243) to D: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
- I305 (≠ L266) to N: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
- L342 (≠ F305) to P: in LCHAD deficiency; dbSNP:rs137852772
- E510 (= E475) active site, For hydroxyacyl-coenzyme A dehydrogenase activity; to Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769
1wdmA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form i (native3) (see paper)
35% identity, 97% coverage: 14:725/737 of query aligns to 16:707/707 of 1wdmA
- active site: A69 (= A67), N89 (≠ K93), N93 (≠ T102), G117 (= G126), E120 (= E129), P139 (= P150), E140 (= E151), P147 (= P158), G148 (= G159), S430 (= S442), H451 (= H463), E463 (= E475), N501 (= N513)
- binding acetyl coenzyme *a: K142 (= K153), D297 (≠ M308), M459 (= M471), N501 (= N513), P534 (= P546), Y652 (≠ L669), L658 (≠ P675)
- binding nicotinamide-adenine-dinucleotide: G321 (= G333), A322 (= A334), I324 (≠ F336), M325 (= M337), D344 (= D356), V401 (= V413), E403 (= E415), N428 (= N440), S430 (= S442), N454 (≠ S466)
1wdlA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form ii (native4) (see paper)
35% identity, 97% coverage: 14:725/737 of query aligns to 16:715/715 of 1wdlA
- active site: A69 (= A67), N89 (≠ R100), N93 (vs. gap), G117 (= G126), E120 (= E129), P139 (= P150), E140 (= E151), P147 (= P158), G148 (= G159), S430 (= S442), H451 (= H463), E463 (= E475), N501 (= N513)
- binding nicotinamide-adenine-dinucleotide: A322 (= A334), I324 (≠ F336), M325 (= M337), D344 (= D356), I345 (≠ R357), A400 (= A412), V401 (= V413), E403 (= E415), N428 (= N440), T429 (= T441), S430 (= S442)
P28793 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Pseudomonas fragi (see paper)
35% identity, 97% coverage: 14:725/737 of query aligns to 16:715/715 of P28793
Query Sequence
>SMc02227 FitnessBrowser__Smeli:SMc02227
MSYTNFKIETDADGIALVTWDMPDKSMNVFTEEVMKELDAIIDQTTADPAVKGVVITSGK
SSFSGGADLSMIKSMFTFQAEERKKDPDNAARKLFDLVGRMTGLFRKLETSGKPWVSAIN
GTCMGGAFEMSLACHGRVASNAKSVKIALPEVKVGIFPGAGGTQRVPRLTNTQDALQMMT
TGSSLTPARAKAMGLVHEVVDPDKLIEAAKAMIKNGLKPVQPWDEKGFKLPGGGIWTPAS
AQLWPAASGILRRETYGNYPGAIAILKCVYEGLQVPFDTALKIEQRYFTEILQTTEAFSM
IRSLFVSMQELGKGARRPAGVPKTELKKVGVVGAGFMGASIAYVTAAGGIPVTLIDRDME
AAEKGKTHSEGLVKDAIGKGRLTKEEGEALLSRITPSADYGDLRDAGLVIEAVFEDRQVK
KDVIEKVEAVIAEDAIFASNTSTLPITGLARNSKRPDRFIGIHFFSPVEKMMLTEVILGK
ETGDRALAAALDYVAAIRKTPIVVNDTRGFYVNRCVFRYIHEAYDMLIEGVPPAMIENAA
KMAGMPVGPLSLNDEVAIDLSQKILKAAVADLGDKAVDLRHMALIDKMVDELDRRGRKNG
KGFYEYPAKPARKYLWPGLKELYPQKDAGEIEVGVLKQRLLVTIALEAARTIEEGIVTDP
READVGSILGFGFAPYTGGTLSYIDGMGAKAFVELCEKLAADHGDHFRPTPLLKEMAEKG
ETFYGRFDPYGAGQKAA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory