SitesBLAST
Comparing SMc02227 SMc02227 fatty oxidation complex enoyl-COA hydratase/3-hydroxyacyl-COA dehydrogenase/3-hydroxybutyryl-COA epimerase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4b3iA Crystal structure of mycobacterium tuberculosis fatty acid beta-oxidation complex with coenzymea bound at the hydratase active sites (see paper)
43% identity, 96% coverage: 1:709/737 of query aligns to 12:725/731 of 4b3iA
- active site: G79 (≠ A67), E100 (≠ G99), R104 (≠ G103), G127 (= G126), E130 (= E129), P151 (= P150), E152 (= E151), G160 (= G159), S452 (= S442), H473 (= H463), E485 (= E475), S523 (≠ N513)
- binding adenosine-5'-diphosphate: Q640 (≠ E630), P641 (≠ I631), P642 (≠ E632), L643 (≠ V633), Q644 (≠ G634)
- binding coenzyme a: S37 (= S26), T38 (≠ M27), V40 (= V29), A77 (≠ G65), G79 (≠ A67), D80 (= D68), V81 (≠ I72), K82 (= K73), L125 (≠ M124), P151 (= P150), E152 (= E151), R186 (≠ S184), F315 (= F305), Q319 (= Q309)
7o4uA Structure of the alpha subunit of mycobacterium tuberculosis beta- oxidation trifunctional enzyme in complex with oxidized nicotinamide adenine dinucleotide (see paper)
43% identity, 96% coverage: 4:709/737 of query aligns to 2:705/711 of 7o4uA
- binding nicotinamide-adenine-dinucleotide: G323 (= G333), A324 (= A334), G325 (= G335), M326 (≠ F336), M327 (= M337), K345 (≠ I355), D346 (= D356), V347 (≠ R357), A402 (= A412), V403 (= V413), F404 (= F414), E405 (= E415), N430 (= N440), S432 (= S442), H453 (= H463), S456 (= S466)
6yswA E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
35% identity, 98% coverage: 6:724/737 of query aligns to 4:703/707 of 6yswA
- active site: A66 (= A67), I71 (= I72), A84 (= A91), Q88 (≠ F95), G112 (= G126), E115 (= E129), P136 (= P150), E137 (= E151), G145 (= G159), D264 (≠ K282), S422 (= S442), H443 (= H463), E455 (= E475), N493 (= N513)
- binding coenzyme a: E23 (≠ K25), M25 (= M27), T27 (≠ V29), A64 (≠ G65), A66 (= A67), D67 (= D68), I68 (≠ L69), N69 (≠ S70), P136 (= P150), E137 (= E151), L140 (≠ V154), F286 (= F305), F287 (≠ V306), T290 (≠ Q309), K293 (≠ G312)
P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
33% identity, 98% coverage: 2:724/737 of query aligns to 39:762/763 of P40939
- V282 (≠ L243) to D: in MTPD; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
- I305 (≠ L266) to N: in MTPD; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
- L342 (≠ F305) to P: in LCHAD deficiency; dbSNP:rs137852772
- E510 (= E475) active site, For hydroxyacyl-coenzyme A dehydrogenase activity; to Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769
1wdmA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form i (native3) (see paper)
35% identity, 97% coverage: 14:725/737 of query aligns to 16:707/707 of 1wdmA
- active site: A69 (= A67), N89 (≠ K93), N93 (≠ T102), G117 (= G126), E120 (= E129), P139 (= P150), E140 (= E151), P147 (= P158), G148 (= G159), S430 (= S442), H451 (= H463), E463 (= E475), N501 (= N513)
- binding acetyl coenzyme *a: K142 (= K153), D297 (≠ M308), M459 (= M471), N501 (= N513), P534 (= P546), M535 (≠ V547), V544 (= V556), V651 (≠ I668), Y652 (≠ L669), G655 (= G672), L658 (≠ P675)
- binding nicotinamide-adenine-dinucleotide: G321 (= G333), A322 (= A334), I324 (≠ F336), M325 (= M337), K343 (≠ I355), D344 (= D356), I345 (≠ R357), A400 (= A412), V401 (= V413), E403 (= E415), K408 (= K420), N428 (= N440), T429 (= T441), S430 (= S442), F452 (= F464), N454 (≠ S466)
1wdlA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form ii (native4) (see paper)
35% identity, 97% coverage: 14:725/737 of query aligns to 16:715/715 of 1wdlA
- active site: A69 (= A67), N89 (≠ R100), N93 (vs. gap), G117 (= G126), E120 (= E129), P139 (= P150), E140 (= E151), P147 (= P158), G148 (= G159), S430 (= S442), H451 (= H463), E463 (= E475), N501 (= N513)
- binding nicotinamide-adenine-dinucleotide: G321 (= G333), A322 (= A334), I324 (≠ F336), M325 (= M337), D344 (= D356), I345 (≠ R357), A400 (= A412), V401 (= V413), V402 (≠ F414), E403 (= E415), K408 (= K420), V411 (= V423), N428 (= N440), T429 (= T441), S430 (= S442)
P28793 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Pseudomonas fragi (see paper)
35% identity, 97% coverage: 14:725/737 of query aligns to 16:715/715 of P28793