Comparing SMc02442 SMc02442 hydrolase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q94JV5 Deaminated glutathione amidase, chloroplastic/cytosolic; dGSH amidase; Nitrilase-like protein 2; Protein nitrilase 1 homolog; AtNit1; Protein Nit1 homolog; EC 3.5.1.128 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
40% identity, 92% coverage: 2:262/285 of query aligns to 36:297/307 of Q94JV5
Sites not aligning to the query:
Q9NQR4 Omega-amidase NIT2; Nitrilase homolog 2; EC 3.5.1.3 from Homo sapiens (Human) (see 2 papers)
37% identity, 94% coverage: 2:269/285 of query aligns to 3:266/276 of Q9NQR4
P47016 Deaminated glutathione amidase; dGSH amidase; Nitrilase homolog 1; EC 3.5.1.128 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
31% identity, 93% coverage: 4:268/285 of query aligns to 7:301/307 of P47016
4hg5A Structural insights into yeast nit2: wild-type yeast nit2 in complex with oxaloacetate (see paper)
31% identity, 93% coverage: 4:268/285 of query aligns to 4:298/304 of 4hg5A
4hg3A Structural insights into yeast nit2: wild-type yeast nit2 in complex with alpha-ketoglutarate (see paper)
31% identity, 93% coverage: 4:268/285 of query aligns to 4:298/304 of 4hg3A
4hgdA Structural insights into yeast nit2: c169s mutant of yeast nit2 in complex with an endogenous peptide-like ligand (see paper)
31% identity, 93% coverage: 4:268/285 of query aligns to 3:294/299 of 4hgdA
3klcB Crystal structure of hyperthermophilic nitrilase (see paper)
33% identity, 89% coverage: 4:258/285 of query aligns to 2:243/261 of 3klcB
Sites not aligning to the query:
3klcA Crystal structure of hyperthermophilic nitrilase (see paper)
33% identity, 89% coverage: 4:258/285 of query aligns to 2:243/261 of 3klcA
Q9UYV8 Nitrilase; PaNit; EC 3.5.5.1 from Pyrococcus abyssi (strain GE5 / Orsay) (see paper)
33% identity, 89% coverage: 4:258/285 of query aligns to 3:244/262 of Q9UYV8
6ypaB The c146a variant of an amidase from pyrococcus horikoshii with bound glutaramide
33% identity, 84% coverage: 19:258/285 of query aligns to 26:251/269 of 6ypaB
7ovgA The c146a variant of an amidase from pyrococcus horikoshii with bound acetamide (see paper)
33% identity, 84% coverage: 19:258/285 of query aligns to 20:245/263 of 7ovgA
5h8iC Crystal structure of medicago truncatula n-carbamoylputrescine amidohydrolase (mtcpa) in complex with n-(dihydroxymethyl)putrescine (see paper)
26% identity, 89% coverage: 6:258/285 of query aligns to 12:274/301 of 5h8iC
5h8jB Crystal structure of medicago truncatula n-carbamoylputrescine amidohydrolase (mtcpa) in complex with cadaverine (see paper)
26% identity, 89% coverage: 6:258/285 of query aligns to 8:270/297 of 5h8jB
5h8lB Crystal structure of medicago truncatula n-carbamoylputrescine amidohydrolase (mtcpa) c158s mutant in complex with putrescine (see paper)
25% identity, 89% coverage: 6:258/285 of query aligns to 9:271/298 of 5h8lB
Q44185 N-carbamoyl-D-amino acid hydrolase; D-N-alpha-carbamilase; EC 3.5.1.77 from Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter) (see paper)
27% identity, 89% coverage: 16:269/285 of query aligns to 14:294/304 of Q44185
1uf8A Crystal structure of c171a/v236a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-phenylalanine
27% identity, 89% coverage: 16:269/285 of query aligns to 13:293/303 of 1uf8A
1uf7A Crystal structure of c171a/v236a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-valine
27% identity, 89% coverage: 16:269/285 of query aligns to 13:293/303 of 1uf7A
1uf5A Crystal structure of c171a/v236a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-methionine
27% identity, 89% coverage: 16:269/285 of query aligns to 13:293/303 of 1uf5A
8hpcC Crystal structure of c171a mutant of n-carbamyl-d-amino acid amidohydrolase complexed with n-carbamyl-d-hydroxyphenylglycine
27% identity, 89% coverage: 16:269/285 of query aligns to 13:293/303 of 8hpcC
4iztA The e41q mutant of the amidase from nesterenkonia sp. An1 showing covalent addition of the acetamide moiety of fluoroacetamide at the active site cysteine
28% identity, 87% coverage: 14:261/285 of query aligns to 22:258/263 of 4iztA
>SMc02442 SMc02442 hydrolase
MTFKAAAVQICSGVDPAGNAETMAKLVREAASRGATYVQTPEMTGAVQRDRTGLRSVLKD
GENDVVVREASRLARELGIYLHVGSTPIARADGKIANRGFLFGPDGAKICDYDKIHMFDV
DLENGESWRESAAYHPGNTARTADLPFGKLGFSICYDVRFPELFRQQAVAGAEIMSVPAA
FTRQTGEAHWEILLRARAIENGLFVIAAAQAGTHEDGRETFGHSMIVDPWGRVLAEAGAT
GEEIIVAEIDVAAVHAARAKIPNLRNARSFVLDEVVPVGKGGAAA
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SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory