SitesBLAST

T49 (= T49) mutation T->F,W: Increases uptake of both methylarsenite and methylarsenate and exhibits higher sensitivity to methylarsenite.
V177 (= V177) mutation to I: Decreases methylarsenite transport activity and results in higher resistance to methylarsenite. Slight decrease in methylarsenate transport activity.; mutation to R: Increases methylarsenite transport activity, leading to lower resistance to methylarsenite. Nearly complete loss of methylarsenate transport activity.

P08995 Nodulin-26; N-26 from Glycine max (Soybean) (Glycine hispida) (see paper)
35% identity, 44% coverage: 8:109/233 of query aligns to 38:137/271 of P08995

query
sites
P08995

R

Q

R

K

C

L

V

V

A

A

E

E

A

A

L

V

G

G

T

T

G

Y

L

F

L

L

V

I

A

F

A

A

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G

V

C

G

A

S

S

G

L

I

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M

V

A

N

D

E

A

N

L

Y

T

Y

A

-

D

-

D

N

A

M

L

I

A

T

L

F

V

P

A

G

N

I

T

A

I

I

A

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W

G

G

A

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L

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L

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V

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Y

I

T

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G

G

P

H

L

I

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S

G

G

A

G

H

H

F

F

N

N

P

P

A

A

V

V

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T

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I

V

A

F

F

A

A

L

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S

T

G

R

R

R

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F

T

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R

L

R

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D

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C

V

A

P

A

A

Y

Y

V

V

I

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A

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Q

V

L

A

L

G

G

A

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I

I

A

L

G

A

T

S

A

G

L

T

A

L

H

R

L

L

M

L

F

F

Sites not aligning to the query:

262 modified: Phosphoserine; by CPK

3nkaA Crystal structure of aqpz h174g,t183f (see paper)
33% identity, 45% coverage: 5:108/233 of query aligns to 2:104/230 of 3nkaA

query
sites
3nkaA

D

H

L

M

T

F

R

R

R

K

C

L

V

A

A

A

E

E

A

C

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F

G

G

T

T

G

F

L

W

L

L

V

V

A

F

A

G

V

G

V

C

G

G

S

S

G
x
A

I

V

M

L

A

A

D

A

A

G

L

F

T

P

A

-

D

E

D

L

A

G

L

I

A

G

L

F

V

A

A

G

N
x
V

T

A

I

L

A

A

T

F

G

G

A

L

I

T

L

V

V

L

V

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L

M

V

A

T

F

I

A

L

V

G

G

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H

L

I

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S

G

G

A

G

H

H

F

F

N

N

P

P

A

A

V

V

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T

L

I

V

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F

L

A

W

L

A

S

G

G

G

R

R

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F

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R

A

R

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C

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A

V

A

G

Y

Y

V

V

I

I

A

A

Q

Q

V

V

A

V

G

G

A

G

I

I

A

V

G

A

T

A

A

A

L

L

A

L

H

Y

L

L

M

I

binding glycerol: A25 (≠ G28), V41 (≠ N45)

Sites not aligning to the query:

binding glycerol: 180, 184

2o9eA Crystal structure of aqpz mutant t183c complexed with mercury (see paper)
33% identity, 44% coverage: 6:108/233 of query aligns to 3:104/232 of 2o9eA

query
sites
2o9eA

L

M

T

F

R

R

R

K

C

L

V

A

A

A

E

E

A

S

L

F

G

G

T

T

G

F

L

W

L

L

V

V

A

F

A

G

V

G

V

S

G

G

S

S

G

A

I

V

M

L

A

A

D

A

A

G

L

F

T

P

A

-

D

E

D

L

A

G

L

I

A

G

L

F

V

A

A

G

N

V

T

A

I

L

A

A

T

F

G

G

A

L

I

T

L

V

V

L

V

T

L

M

V

A

T

F

I

A

L

V

G

G

P

H

L

I

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S

G

G

A

G

H

H

F

F

N

N

P

P

A

A

V

V

S

T

L

I

V

G

F

L

A

W

L

A

S

G

G

G

R

R

L

F

T

P

R

A

R

K

D

E

C

V

A

V

A

G

Y

Y

V

V

I

I

A

A

Q

Q

V

V

A

V

G

G

A

G

I

I

A

V

G

A

T

A

A

A

L

L

A

L

H

Y

L

L

M

I

Sites not aligning to the query:

binding mercury (ii) ion: 140, 179

P60844 Aquaporin Z; Bacterial nodulin-like intrinsic protein; Water channel AqpZ from Escherichia coli (strain K12) (see paper)
33% identity, 44% coverage: 6:108/233 of query aligns to 1:102/231 of P60844

query
sites
P60844

L

M

T

F

R

R

R

K

C

L

V

A

A

A

E

E

A
x
C

L

F

G

G

T

T

G

F

L

W

L

L

V

V

A

F

A

G

V

G

V
x
C

G

G

S

S

G

A

I

V

M

L

A

A

D

A

A

G

L

F

T

P

A

-

D

E

D

L

A

G

L

I

A

G

L

F

V

A

A

G

N

V

T

A

I

L

A

A

T

F

G

G

A

L

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T

L

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V

L

V

T

L

M

V

A

T

F

I

A

L

V

G

G

P

H

L

I

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S

G

G

A

G

H

H

F

F

N

N

P

P

A

A

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V

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L

I

V

G

F

L

A

W

L

A

S

G

G

G

R

R

L

F

T

P

R

A

R

K

D

E

C

V

A

V

A

G

Y

Y

V

V

I

I

A

A

Q

Q

V

V

A

V

G

G

A

G

I

I

A

V

G

A

T

A

A

A

L

L

A

L

H

Y

L

L

M

I

C9 (≠ A14) mutation to S: No effect.
C20 (≠ V25) mutation to S: Loss of oligomerization; no alteration of water permeability.

Sites not aligning to the query:

183 T→C: No effect.
189 mutation R->V,S: Loss of function.

B1VB61 Propanediol uptake facilitator PduF from Citrobacter freundii (see paper)
35% identity, 45% coverage: 5:109/233 of query aligns to 4:106/269 of B1VB61

query
sites
B1VB61

D

S

L

L

T

K

R

A

R

Q

C

C

V

I

A

A

E

E

A

F

L

L

G

G

T

T

G

G

L

L

L

F

V

L

A

-

V

F

G

G

S

I

G

G

I

C

M

L

A

S

D

A

A

L

L

K

T

V

A

A

D

G

D

A

A

S

L

L

A

G

L

L

V

W

A

E

N

I

T

C

I

I

A

I

T

W

G

G

A

L

I

G

L

I

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S

V

L

L

A

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Y

I

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A

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G

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S

G

G

A

A

H

H

F

L

N
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N

P
|
P

A
|
A

V

I

S

T

L

I

V

A

F

L

A

W

L

L

S

F

G

A

R

C

L

F

T

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R

G

R

R

D

K

C

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A

L

A

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Y

Y

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I

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A

A

Q

Q

V

V

A

A

G

G

A

A

I

F

A

G

G

G

T

A

A

L

L

L

A

A

H

Y

L

L

M

L

F

Y

NPA 66:68 (= NPA 69:71) NPA 1

Sites not aligning to the query:

201:203 NPA 2

P37451 Propanediol uptake facilitator PduF from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
33% identity, 45% coverage: 5:110/233 of query aligns to 4:107/264 of P37451

query
sites
P37451

D

S

L

L

T

K

R

A

R

Q

C

C

V

G

A

A

E

E

A

F

L

L

G

G

T

T

G

G

L

L

L

F

V

L

A

-

V

F

G

G

S

I

G

G

I

C

M

L

A

S

D

A

A

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L

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T

V

A

A

D

G

D

A

A

S

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L

A

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L

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W

A

E

N

I

T

C

I

I

A

I

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G

G

A

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G

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L

N
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N

P
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P

A
|
A

V

V

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T

L

I

V

A

F

L

A

W

L

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S

F

G

A

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A

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A

A

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Q

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F

A

A

G

G

A

A

I

F

A

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G

T

A

A

L

L

L

A

A

H

Y

L

V

M

L

F

Y

D

S

NPA 66:68 (= NPA 69:71) NPA 1

Sites not aligning to the query:

201:203 NPA 2

I1CR68 Aquaporin-1 from Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) (Mucormycosis agent) (Rhizopus arrhizus var. delemar) (see paper)
30% identity, 44% coverage: 8:110/233 of query aligns to 59:160/306 of I1CR68

query
sites
I1CR68

R

R

R

E

C

F

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L

A

A

E

E

A

F

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I

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G

T

T

G

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L

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V

A

-

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C

A

A

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-

Q

A

T

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H

A

I

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E

A

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K

A

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L

A

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S

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A

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G

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N

N

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A

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F

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Y

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A

A

Q

Q

V

L

A

L

G

G

A

A

I

F

A

A

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G

T

A

A

A

L

L

A

L

H

Y

L

I

M

I

F

I

D

E

Sites not aligning to the query:

275 H→A: Affects pH sensing; when associated with A-85.

P0AER0 Glycerol uptake facilitator protein; Aquaglyceroporin; Glycerol facilitator from Escherichia coli (strain K12) (see 3 papers)
31% identity, 47% coverage: 2:111/233 of query aligns to 3:115/281 of P0AER0

query
sites
P0AER0

Q

Q

E

T

F

S

D

T

L

L

T

K

R

G

R

Q

C

C

V

I

A

A

E

E

A

F

L

L

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G

T

T

G

G

L

L

V

I

A

-

V

F

G

G

S

V

G

G

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M

V

A

A

D

A

A

L

L

K

T

V

A

A

D

G

D

A

A

S

L

F

A

G

L

Q

V

W

A

E

N

I

T

S

I

V

A

I

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W

G

G

A

L

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G

L

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V

A

V

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L

A

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Y

I

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L

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G

A

P

G

L

V

S

S

G

G

A

A

H
|
H

F
x
L

N
|
N

P

P

A

A

V

V

S

T

L

I

V

A

F

L

A

W

L

L

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F

G

A

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L

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D

R

K

R

R

D

K

C

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A

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A

P

Y

F

V

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I

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A

S

Q

Q

V

V

A

A

G

G

A

A

I

F

A

C

G

A

T

A

A

A

L

L

A

V

H

Y

-

G

-

L

-

Y

-

N

L

L

M

F

F

F

D

D

L

F

HLN 66:68 (≠ HFN 67:69) binding

Sites not aligning to the query:

138 binding
199:201 binding
203 binding
206 binding
236:237 PL→FW: No detectable water or glycerol permeability.

1fx8A Crystal structure of the e. Coli glycerol facilitator (glpf) with substrate glycerol (see paper)
32% identity, 45% coverage: 6:111/233 of query aligns to 2:110/254 of 1fx8A

query
sites
1fx8A

L

L

T

K

R

G

R

Q

C

C

V

I

A

A

E

E

A

F

L

L

G

G

T

T

G

G

L

L

V

I

A

-

V

F

G

G

S

V

G

G

I

C

M

V

A

A

D

A

A

L

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T

V

A

A

D

G

D

A

A

S

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F

A

G

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A

E

N

I

T

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I

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A

I

T
x
W

G

G

A

L

I

G

L

V

V

A

V

M

L

A

V

I

T

Y

I

L

L

T

G

A

P

G

L

V

S

S

G

G

A

A

H

H

F

L

N
|
N

P

P

A

A

V

V

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T

L

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A

F

L

A

W

L

L

S

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A

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F

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D

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K

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R

D

K

C

V

A

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A

P

Y

F

V

I

I

V

A

S

Q

Q

V

V

A

A

G

G

A

A

I

F

A

C

G

A

T

A

A

A

L

L

A

V

H

Y

-

G

-

L

-

Y

-

N

L

L

M

F

F

F

D

D

L

F

binding glycerol: W43 (≠ T49), N63 (= N69)

Sites not aligning to the query:

binding glycerol: 194, 195, 196, 198, 201

Q6Z2T3 Aquaporin NIP2-1; Low silicon protein 1; NOD26-like intrinsic protein 2-1; OsNIP2;1; Silicon influx transporter LSI1 from Oryza sativa subsp. japonica (Rice) (see paper)
30% identity, 79% coverage: 6:190/233 of query aligns to 47:236/298 of Q6Z2T3

query
sites
Q6Z2T3

L

L

T

L

R

K

C

V

V

V

A

S

E

E

A

V

L

V

G

A

T

T

G

F

L

L

V

V

A

F

A

M

V

T

V

C

G

G

S

A

-

A

G

G

I

I

M

S

A

G

D

S

A

D

L

L

T

S

A

R

D

-

A

-

L

I

A

S

L

Q

V

L

A

G

N

Q

T

S

I

I

A

A

T

G

G

G

A

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I

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I

A

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G

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G

A

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N

N

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A

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F

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A

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Y

V

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I

A

A
|
A

Q

Q

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A

T

G

G

A

A

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C

G

A

T

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A

F

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A

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H

-

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P

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D

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A

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H

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W

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H

S

S

-

L

-

V

E

E

G

V

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A

V

A

T

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F

G

N

L

M

V

M

A

F

T

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I

T

L

L

A

A

G

-

I

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R

A

F

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E

R

A

A

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E

L

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A

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G

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Y

A

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G

A

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A

A

Y

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W

C

F

I

T

T

A

S

-

I

-

F

-

A

-

G

-

A

-

I

S

S

T

G

S

G

F

S

A

M

N

N

P

P

A

A

V

R

A

T

L

L

A

G

R

P

S

A

F

L

-

A

T

S

N

N

T

K

F

F

S

D

G

G

I

L

A132 (= A93) mutation to T: In lsi; impairs silicon uptake. Grain discoloration. Reduces grain yield 10-fold.

Q41951 Aquaporin TIP2-1; Delta-tonoplast intrinsic protein; Delta-TIP; Tonoplast intrinsic protein 2-1; AtTIP2;1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
42% identity, 40% coverage: 8:100/233 of query aligns to 19:114/250 of Q41951

query
sites
Q41951

R

R

R

A

C

Y

V

L

A

A

E

E

A

F

L

I

G

S

T

T

G

L

L

L

L

F

V

V

A

F

A

A

V

G

V

V

G

G

S

S

G

A

I

I

M

A

A

Y

D

A

A

K

L

L

T

T

A

S

D

D

D

A

A

A

L

L

-

D

-

T

-

P

A

G

L

L

V

V

A

A

N

I

T

A

I

V

A

C

T

H

G

G

A

F

I

A

L

L

V

F

V

V

L

A

V

V

T

A

I

I

L

G

G

A

P

N

L

I

S

S

G

G

A

G

H

H

F

V

N

N

P

P

A

A

V

V

S

T

L

F

V

G

F

L

A

A

L

V

S

G

G

G

R

Q

L

I

T

T

R

V

R

I

D

T

C

G

A

V

A

F

Y

Y

V

W

I

I

A

A

Q

Q

V

L

A

L

G

G

A

S

I

T

A

A

Sites not aligning to the query:

116 C→S: Reduces the mercury-sensitivity.

8ct2D Local refinement of aqp1 tetramer (c1; refinement mask included d1 of protein 4.2 and ankyrin-1 ar1-5) in class 2 of erythrocyte ankyrin-1 complex (see paper)
36% identity, 42% coverage: 8:104/233 of query aligns to 10:109/247 of 8ct2D

query
sites
8ct2D

R

R

R

A

C

V

V

V

A

A

E

E

A

F

L

L

G

A

T

T

G

T

L

L

L

F

V

V

A

F

A

I

V

S

V

I

G

G

S

S

G

A

I

L

M

G

A

F

D

K

A

Y

L

P

T

V

A

G

D

N

A

Q

L

T

A

A

L

V

V

Q

A

D

N

N

-

V

-

K

-

V

T

S

I

L

A

A

T

F

G

G

A

L

I

S

L

I

V

A

V

T

L

L

V

A

T

Q

I

S

L

V

G

G

P

H

L

I

S

S

G

G

A

A

H

H

F

L

N

N

P

P

A

A

V

V

S

T

L

L

V

G

F

L

A

L

L

L

S

S

G

C

R

Q

L

I

T

S

R

I

R

F

D

R

C

A

A

L

A

M

Y

Y

V

I

I

I

A

A

Q

Q

V

C

A

V

G

G

A

A

I

I

A

V

G

A

T

T

A

A

L

I

Sites not aligning to the query:

binding cholesterol: 134, 138

Q96PS8 Aquaporin-10; AQP-10; Aquaglyceroporin-10; Small intestine aquaporin from Homo sapiens (Human) (see 2 papers)
28% identity, 45% coverage: 6:110/233 of query aligns to 20:123/301 of Q96PS8

query
sites
Q96PS8

L

L

T

A

R

R

R

Q

C

C

V

L

A

A

E
|
E

A

F

L

L

G

G

T

-

G

-

L

V

L

F

V

V

A

L

A

M

V

L

G

T

S

Q

G

G

I

A

M

V

A

A

D

Q

A

A

L

V

T

T

A

S

D

G

D

E

A

T

L

K

A

G

L

N

V

F

A

F

N

T

T

M

I

F

A

L

T

A

G

G

A

S

I

L

L

A

V

V

V

T

L

I

-

A

V

I

T

Y

I

V

L
x
G

G

G

P

N

L

V

S
|
S

G

G

A

A

H
|
H

F

L

N

N

P

P

A

A

V
x
F

S

S

L

L

V

A

F

M

A

C

L

I

S

V

G

G

R
|
R

L

L

T

P

R

W

R

V

D

K

C

L

A

P

A

I

Y

Y

V

I

I

L

A

V

Q

Q

V

L

A

L

G

S

A

A

I

F

A

C

G

A

T

S

A

G

L

A

A

T

H

Y

L

V

M

L

F

Y

D

H

E27 (= E13) mutation to Q: Abolishes permeability to glycerol.
G73 (≠ L60) mutation to A: Increased permeability to glycerol at acidic pH.; mutation to F: Abolishes permeability to glycerol.
S77 (= S64) mutation S->A,D: Nearly abolishes permeability to glycerol.
H80 (= H67) mutation to A: Abolishes permeability to glycerol.
F85 (≠ V72) mutation to A: Nearly abolishes permeability to glycerol.
R94 (= R81) mutation to A: Abolishes permeability to glycerol.

Sites not aligning to the query:

133 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→Q: Abolishes N-glycosylation.

6f7hC Crystal structure of human aqp10 (see paper)
28% identity, 45% coverage: 6:110/233 of query aligns to 5:108/253 of 6f7hC

query
sites
6f7hC

L

L

T

A

R

R

R

Q

C

C

V

L

A

A

E

E

A

F

L

L

G

G

T

-

G

-

L

V

L

F

V

V

A

L

A

M

V

L

G

T

S

Q

G

G

I

A

M

V

A

A

D

Q

A

A

L

V

T

T

A

S

D

G

D

E

A

T

L

K

A

G

L

N

V

F

A

F

N

T

T

M

I

F

A

L

T

A

G

G

A

S

I

L

L

A

V

V

V

T

L

I

-

A

V
x
I

T

Y

I

V

L

G

G

G

P

N

L

V

S

S

G

G

A

A

H

H

F

L

N

N

P

P

A

A

V

F

S

S

L

L

V

A

F

M

A

C

L

I

S

V

G

G

R

R

L

L

T

P

R

W

R

V

D

K

C

L

A

P

A

I

Y

Y

V

I

I

L

A

V

Q

Q

V

L

A

L

G

S

A

A

I

F

A

C

G

A

T

S

A

G

L

A

A

T

H

Y

L

V

M

L

F

Y

D

H

binding glycerol: I55 (≠ V57)

Sites not aligning to the query:

binding glycerol: 199

P41181 Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct from Homo sapiens (Human) (see 12 papers)
36% identity, 50% coverage: 1:116/233 of query aligns to 4:112/271 of P41181

query
sites
P41181

M

L

Q

R

E

S

F

I

D

A

L

F

T

S

R

R

R

A

C

V

V

F

A

A

E

E

A

F

L

L

G

A

T

T

G

L

L

L

L

F

V

V

A

F

V

G

V

L

G

G

S

S

G

-

I

-

M

-

A

-

D

-

A

A

L

L

T

N

A

W

D

P

D

Q

A

A

L

L

A

P

L

S

V

V

A

L

N

Q

-

I

T

A

I

M

A

A

T

F

G

G

A

L

I

G

L

I

V

G

V

T

L

L

V

V

T

Q

I

A

L

L

G

G

P

H

L

I

S

S

G
|
G

A

A

H

H

F

I

N

N

P

P

A

A

V

V

S

T

L

V

V

A

F

C

A

L

L

V

S
x
G

G
x
C

R

H

L

V

T

S

R

V

R

L

D

R

C

A

A

A

A

F

Y

Y

V

V

I

A

A

A

Q

Q

V

L

A

L

G

G

A

A

I

V

A

A

G

G

T

A

A

A

L

L

A

L

H

H

L

-

M

-

F

-

D

E

L

I

P

T

P

P

L

A

D

D

M

I

G64 (= G65) to R: in NDI2; loss of water channel activity; dbSNP:rs104894326
G78 (≠ S79) mutation to A: Does not affect interaction with MIAC; when associated with A-79.
C79 (≠ G80) mutation to A: Does not affect interaction with MIAC; when associated with A-78.

Sites not aligning to the query:

148 S→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; S→D: Retained in the endoplasmic reticulum.
187 R → C: in NDI2; loss of water channel activity; mutant protein does not fold properly; dbSNP:rs104894328
190 A → T: in NDI2; mutant protein does not fold properly and is not functional; dbSNP:rs104894341
194 V → I: in dbSNP:rs772051028
216 S → P: in NDI2; loss of water channel activity; dbSNP:rs104894329
217 L→A: Abolishes interaction with MIAC; when associated with A-221.
221 Y→A: Abolishes interaction with MIAC; when associated with A-217.
229 S→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; S→D: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
231 S→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; S→D: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
232 E→A: Reduces interaction with MIAC.
244 T→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; T→E: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
254 R → L: in NDI2; results in the loss of arginine vasopressin-mediated phosphorylation at S-256; R → Q: in NDI2; exerts a dominant-negative effect on wild-type-AQP2 in that it interferes with its trafficking to the apical membrane; is a loss of function instead of a gain of function mutation on dominant nephrogenic diabetes insipidus
256 modified: Phosphoserine; by PKA; S→A: Retained in vesicles.; S→D: Expressed in the apical membrane.
258 E → K: in NDI2; retained in the Golgi compartment; dbSNP:rs104894332
262 P → L: in NDI2; mutant protein folds properly and is functional but is retained in intracellular vesicles; able to assemble into tetramers with wild-type AQP2 that properly localize to the apical membrane; dbSNP:rs104894339; P→A: No effect on expression at the apical cell membrane.

4nefA X-ray structure of human aquaporin 2 (see paper)
35% identity, 50% coverage: 1:116/233 of query aligns to 3:111/239 of 4nefA

query
sites
4nefA

M

L

Q

R

E

S

F

I

D

A

L

F

T

S

R

R

R

A

C

V

V

F

A

A

E

E

A

F

L

L

G

A

T

T

G

L

L

L

L

F

V

V

A

F

V

G

V

L

G

G

S

S

G

-

I

-

M

-

A

-

D

-

A

A

L

L

T

N

A

W

D

P

D

Q

A

A

L

L

-

P

A

S

L

V

V

L

A

Q

N

I

T

A

I

M

A

A

T

F

G

G

A

L

I

G

L

I

V

G

V

T

L

L

V

V

T

Q

I

A

L

L

G

G

P

H

L

I

S

S

G

G

A

A

H

H

F

I

N

N

P

P

A

A

V

V

S

T

L

V

V

A

F
x
C

A

L

L

V

S

G

G

C

R
x
H

L

V

T

S

R

V

R

L

D

R

C

A

A

A

A

F

Y

Y

V

V

I

A

A

A

Q

Q

V

L

A

L

G

G

A

A

I

V

A

A

G

G

T

A

A

A

L

L

A

L

H

H

L

-

M

-

F

-

D

E

L

I

P

T

P

P

L

A

D

D

M

I

binding zinc ion: C74 (≠ F76), H79 (≠ R81)

P06624 Lens fiber major intrinsic protein; Aquaporin-0; MIP26; MP26 from Bos taurus (Bovine) (see 4 papers)
34% identity, 34% coverage: 28:106/233 of query aligns to 27:105/263 of P06624

query
sites
P06624

G

G

I

L

M

G

A

A

D

S

A

L

L

R

T

W

A

A

D

P

D

G

A

P

L

L

A

H

L

V

V

L

A

Q

N

V

T

A

I

L

A

A

T

F

G

G

A

L

I

A

L

L

V

A

V

T

L

L

V

V

T

Q

I

A

L

V

G

G

P

H

L

I

S

S

G

G

A

A

H

H

F

V

N

N

P

P

A

A

V

V

S

T

L

F

V

A

F

F

A

L

L

V

S

G

G

S

R

Q

L

M

T

S

R

L

D

R

C

A

A

I

A

C

Y

Y

V

M

I

V

A

A

Q

Q

V

L

A

L

G

G

A

A

I

V

A

A

G

G

T

A

A

A

L

V

A

L

H

Y

Sites not aligning to the query:

149 Y→G: Increases constitutive water permeability. Abolishes regulation by cytoplasmic calcium levels.; Y→L: Strongly decreases water permeability. Abolishes regulation by cytoplasmic calcium levels.; Y→S: Slightly decreases water permeability, but has a minor effect on the regulation by cytoplasmic calcium levels.
227 L→A: Strongly reduced CALM binding.
227:237 Interaction with CALM
230 V→A: Strongly reduced CALM binding.
235 modified: Phosphoserine
243 modified: Phosphoserine; by PKA
245 modified: Phosphoserine
246 Interaction with BFSP1; modified: Deamidated asparagine
250 interaction with BFSP1

P25818 Aquaporin TIP1-1; Aquaporin TIP; Gamma-tonoplast intrinsic protein; Gamma-TIP; Tonoplast intrinsic protein 1-1; AtTIP1;1; Tonoplast intrinsic protein, root-specific RB7 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
29% identity, 76% coverage: 5:180/233 of query aligns to 18:205/251 of P25818

query
sites
P25818

D

D

L

A

T

L

R

K

R

A

C

A

V

L

A

A

E

E

A

F

L

I

G

S

T

T

G

L

L

I

L

F

V

V

A

V

A

A

V

G

V

S

G

G

S

S

G

G

I

M

M

A

A

F

D

N

A

K

L

L

T

T

A

E

D

N

D

G

A

A

L

T

-

T

-

P

-

S

A

G

L

L

V

V

A

A

N

A

T

A

I

V

A

A

T

H

G

A

A

F

I

G

L

L

V

F

V

V

L

A

V

V

T

S

I

V

L

G

G

A

P

N

L

I

S

S

G

G

A

G

H

H

F

V

N

N

P

P

A

A

V

V

S

T

L

F

V

G

F

A

A

F

L

I

S

G

G

G

R

N

L

I

T

T

R

L

D

R

C

G

A

I

A

L

Y

Y

V

W

I

I

A

A

Q

Q

V

L

A

L

G

G

A

S

I

V

A

V

G

A

T
x
C

A

L

L

I

A

L

H

K

L

F

M

A

-

T

-

G

F

L

D

A

L

V

P

P

P

A

L

F

D

G

M

L

S

S

M

A

K

G

V

V

R

G

T

V

G

L

P

N

A

A

Q

-

W

F

L

V

S

F

E

E

G

I

V

V

A

M

A

T

F

F

G

G

L

L

V

V

A

Y

T

T

I

V

L

Y

A

A

G

T

I

A

R

I

F

D

H

P

R

K

E

N

-

G

-

S

-

L

-

G

-

T

-

I

-

A

-

P

-

I

A

A

V

I

P

G

W

F

L

I

V

V

G

G

L

A

Y

N

I

I

T

L

A

A

A

G

Y

G

W

A

F

F

T

S

A

G

S

A

T

S

S

-

F

-

A

M

N

N

P

P

A

A

V

V

A

A

L

F

A

G

C118 (≠ T102) mutation to T: Strongly reduces the mercury-sensitivity.

P56402 Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct from Mus musculus (Mouse) (see 2 papers)
31% identity, 50% coverage: 1:116/233 of query aligns to 4:112/271 of P56402

query
sites
P56402

M

L

Q

R

E

S

F

I

D

A

L

F

T

S

R

R

R

A

C

V

V

L

A

A

E

E

A

F

L

L

G

A

T

T

G

L

L

L

L

F

V

V

A

F

V

G

V

L

G

G

S

S

G

A

I

L

M

Q

A

-

D

-

A

-

L

-

T

W

A

A

D

S

A

P

L

P

A

S

L

V

V

L

A

Q

N

I

T

A

I

V

A

A

T

F

G

G

A

L

I

G

L

I

V

G

V

T

L

L

V

V

T

Q

I

A

L

L

G

G

P

H

L

V

S

S

G

G

A

A

H

H

F

I

N

N

P

P

A

A

V

V

S

T

L

V

V

A

F

C

A

L

L

V

S

G

G

C

R

H

L

V

T

S

R

F

R

L

D

R

C

A

A

A

A

F

Y

Y

V

V

I

A

A

A

Q

Q

V

L

A

L

G

G

A

A

I

V

A

A

G

G

T

A

A

A

L

I

A

L

H

H

L

-

M

-

F

-

D

E

L

I

P

T

P

P

L

V

D

E

M

I

Sites not aligning to the query:

126 T→M: Does not cause loss of water channel activity, but impairs trafficking from cytoplasmic vesicles to the cell membrane.
256 modified: Phosphoserine; S → L: in cph; loss of a phosphorylation site and loss of trafficking to the apical cell membrane; causes aberrant location at the basolateral cell membrane

Query Sequence

>SMc02648 FitnessBrowser__Smeli:SMc02648
MQEFDLTRRCVAEALGTGLLVAAVVGSGIMADALTADDALALVANTIATGAILVVLVTIL
GPLSGAHFNPAVSLVFALSGRLTRRDCAAYVIAQVAGAIAGTALAHLMFDLPPLDMSMKV
RTGPAQWLSEGVAAFGLVATILAGIRFHREAVPWLVGLYITAAYWFTASTSFANPAVALA
RSFTNTFSGIRPGDLPGFVIAELLGAVCALALMRWLLQPARPIIRQTSPETAP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory