SitesBLAST
Comparing SMc02837 FitnessBrowser__Smeli:SMc02837 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3ijpB Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
55% identity, 98% coverage: 6:272/272 of query aligns to 2:267/267 of 3ijpB
3ijpA Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
55% identity, 98% coverage: 6:271/272 of query aligns to 2:266/266 of 3ijpA
- active site: H155 (= H160), K159 (= K164)
- binding sodium ion: I21 (= I25), Q22 (≠ H26), R24 (≠ T28), V27 (= V31)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), N10 (≠ A14), G11 (= G15), R12 (= R16), M13 (= M17), R35 (= R39), F75 (= F79), S76 (≠ T80), Q77 (≠ A81), A80 (= A84), G98 (= G102), T100 (= T104), G123 (= G127), N124 (= N128), M125 (= M129), F239 (= F244)
4ywjA Crystal structure of 4-hydroxy-tetrahydrodipicolinate reductase (htpa reductase) from pseudomonas aeruginosa
47% identity, 97% coverage: 7:271/272 of query aligns to 3:267/268 of 4ywjA
- active site: H156 (= H160), K160 (= K164)
- binding nicotinamide-adenine-dinucleotide: G11 (= G15), R12 (= R16), M13 (= M17), D35 (≠ E38), R36 (= R39), F76 (= F79), T77 (= T80), V81 (≠ A84), G99 (= G102), T101 (= T104), A124 (≠ G127), N125 (= N128), F126 (≠ M129), R237 (= R241), F240 (= F244)
5tejB Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
44% identity, 98% coverage: 6:272/272 of query aligns to 2:267/269 of 5tejB
- active site: H155 (= H160), K159 (= K164)
- binding 2,5 Furan Dicarboxylic Acid: T100 (= T104), H156 (= H161), K159 (= K164), S164 (= S169), G165 (= G170), T166 (= T171)
- binding nicotinamide-adenine-dinucleotide: G8 (= G12), G11 (= G15), R12 (= R16), M13 (= M17), E34 (= E38), R35 (= R39), F75 (= F79), T76 (= T80), S80 (≠ A84), G98 (= G102), T100 (= T104), P123 (≠ G127), N124 (= N128), Y125 (≠ M129), F239 (= F244)
5tejA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
44% identity, 98% coverage: 6:272/272 of query aligns to 2:267/269 of 5tejA
- active site: H155 (= H160), K159 (= K164)
- binding nicotinamide-adenine-dinucleotide: G8 (= G12), G11 (= G15), R12 (= R16), M13 (= M17), E34 (= E38), R35 (= R39), F75 (= F79), T76 (= T80), S80 (≠ A84), G98 (= G102), T100 (= T104), P123 (≠ G127)
5temA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,6 pyridine dicarboxylic and nadh (see paper)
45% identity, 98% coverage: 6:271/272 of query aligns to 2:266/266 of 5temA
- active site: H155 (= H160), K159 (= K164)
- binding nicotinamide-adenine-dinucleotide: G8 (= G12), G11 (= G15), R12 (= R16), M13 (= M17), E34 (= E38), R35 (= R39), F75 (= F79), T76 (= T80), S80 (≠ A84), G98 (= G102), T100 (= T104), P123 (≠ G127), N124 (= N128), Y125 (≠ M129), F239 (= F244)
- binding pyridine-2,6-dicarboxylic acid: T100 (= T104), P123 (≠ G127), H156 (= H161), K159 (= K164), S164 (= S169), G165 (= G170), T166 (= T171)
P04036 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Escherichia coli (strain K12) (see 3 papers)
44% identity, 100% coverage: 1:272/272 of query aligns to 1:271/273 of P04036
- G12 (= G12) binding
- GRM 15:17 (= GRM 15:17) binding
- RM 16:17 (= RM 16:17) binding
- E38 (= E38) binding
- R39 (= R39) binding
- TR 80:81 (≠ TA 80:81) binding
- GTT 102:104 (= GTT 102:104) binding ; binding
- AANF 126:129 (≠ SGNM 126:129) binding
- F129 (≠ M129) binding
- H159 (= H160) mutation H->A,Q: 135 to 200-fold reduction in catalytic activity.
- K163 (= K164) binding ; mutation K->A,C,Q: 625 to 830-fold reduction in catalytic activity.
- R240 (= R241) binding
- F243 (= F244) binding
1drwA Escherichia coli dhpr/nhdh complex (see paper)
44% identity, 100% coverage: 2:272/272 of query aligns to 1:270/272 of 1drwA
- active site: H158 (= H160), K162 (= K164)
- binding nicotinamide purin-6-ol-dinucleotide: G11 (= G12), G14 (= G15), R15 (= R16), M16 (= M17), E37 (= E38), R38 (= R39), F78 (= F79), T79 (= T80), R80 (≠ A81), G101 (= G102), T102 (= T103), T103 (= T104), A126 (≠ G127), N127 (= N128), F128 (≠ M129), F242 (= F244)
1dihA Three-dimensional structure of e. Coli dihydrodipicolinate reductase (see paper)
44% identity, 100% coverage: 2:272/272 of query aligns to 1:270/272 of 1dihA
- active site: H158 (= H160), K162 (= K164)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G12), G14 (= G15), R15 (= R16), M16 (= M17), R38 (= R39), F78 (= F79), T79 (= T80), R80 (≠ A81), G83 (≠ A84), G101 (= G102), T103 (= T104), N127 (= N128), F128 (≠ M129), R239 (= R241), F242 (= F244)
1drvA Escherichia coli dhpr/acnadh complex (see paper)
44% identity, 99% coverage: 5:272/272 of query aligns to 2:268/270 of 1drvA
- active site: H156 (= H160), K160 (= K164)
- binding 3-acetylpyridine adenine dinucleotide: G9 (= G12), G12 (= G15), R13 (= R16), M14 (= M17), E35 (= E38), F76 (= F79), T77 (= T80), R78 (≠ A81), G81 (≠ A84), G99 (= G102), A124 (≠ G127), F126 (≠ M129), R237 (= R241)
1druA Escherichia coli dhpr/nadh complex (see paper)
44% identity, 99% coverage: 5:272/272 of query aligns to 2:268/270 of 1druA
- active site: H156 (= H160), K160 (= K164)
- binding nicotinamide-adenine-dinucleotide: G9 (= G12), G12 (= G15), R13 (= R16), M14 (= M17), E35 (= E38), R36 (= R39), F76 (= F79), T77 (= T80), R78 (≠ A81), G81 (≠ A84), G99 (= G102), T100 (= T103), T101 (= T104), A124 (≠ G127), N125 (= N128), F126 (≠ M129), F240 (= F244)
1arzA Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
44% identity, 99% coverage: 5:272/272 of query aligns to 2:268/270 of 1arzA
1arzB Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
44% identity, 99% coverage: 5:272/272 of query aligns to 1:267/269 of 1arzB
- active site: H155 (= H160), K159 (= K164)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G8 (= G12), G10 (≠ A14), G11 (= G15), R12 (= R16), M13 (= M17), E34 (= E38), F75 (= F79), T76 (= T80), R77 (≠ A81), G80 (≠ A84), H84 (≠ F88), G98 (= G102), T100 (= T104), A123 (≠ G127), N124 (= N128), F125 (≠ M129), F239 (= F244)
- binding pyridine-2,6-dicarboxylic acid: T100 (= T104), H156 (= H161), K159 (= K164), S164 (= S169), G165 (= G170), T166 (= T171), F239 (= F244)
Q9X1K8 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
30% identity, 97% coverage: 6:269/272 of query aligns to 1:212/216 of Q9X1K8
1vm6B Crystal structure of dihydrodipicolinate reductase (tm1520) from thermotoga maritima at 2.27 a resolution
30% identity, 97% coverage: 6:269/272 of query aligns to 6:217/218 of 1vm6B
- active site: H132 (= H160), K136 (= K164)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), S14 (≠ A14), G15 (= G15), R16 (= R16), M17 (= M17), D37 (= D48), V38 (≠ A49), F53 (= F79), S54 (≠ T80), S55 (≠ A81), E57 (≠ A83), A58 (= A84), G76 (= G102), T78 (= T104), Y101 (≠ G127), N102 (= N128), F103 (≠ M129), F192 (= F244)
5z2fA NADPH/pda bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
27% identity, 95% coverage: 12:269/272 of query aligns to 9:265/265 of 5z2fA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R11 (≠ A14), G12 (= G15), K13 (≠ R16), M14 (= M17), D35 (≠ E38), H36 (≠ R39), K37 (≠ R47), L76 (≠ F79), T77 (= T80), G99 (= G102), T100 (= T103), T101 (= T104), P126 (≠ G127), N127 (= N128), F128 (≠ M129)
- binding pyridine-2,6-dicarboxylic acid: P126 (≠ G127), H155 (= H160), H156 (= H161), K159 (= K164), S164 (= S169), G165 (= G170), T166 (= T171), A215 (≠ G219)
5z2eA Dipicolinate bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
27% identity, 95% coverage: 12:269/272 of query aligns to 9:265/265 of 5z2eA
5eesA Crystal structure of dapb in complex with NADP+ from corynebacterium glutamicum (see paper)
30% identity, 98% coverage: 6:271/272 of query aligns to 2:247/247 of 5eesA
- active site: H133 (= H160), K137 (= K164)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), G11 (= G15), R12 (= R16), V13 (≠ M17), G34 (= G59), V35 (= V60), F53 (= F79), T54 (= T80), G76 (= G102), T78 (= T104), P104 (≠ G127), N105 (= N128), F106 (≠ M129), F220 (= F244)
- binding sulfate ion: H134 (= H161), K137 (= K164), K137 (= K164), G143 (= G170), T144 (= T171)
5eerA Crystal structure of dapb from corynebacterium glutamicum (see paper)
30% identity, 98% coverage: 6:271/272 of query aligns to 2:247/247 of 5eerA
5wolA Crystal structure of dihydrodipicolinate reductase dapb from coxiella burnetii
30% identity, 66% coverage: 73:252/272 of query aligns to 48:213/230 of 5wolA
- active site: H133 (= H160), K137 (= K164)
- binding pyridine-2-carboxylic acid: P104 (≠ G127), T144 (= T171), K147 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: F54 (= F79), T55 (= T80), T56 (≠ A81), S59 (≠ A84), G77 (= G102), T78 (= T103), T79 (= T104), P104 (≠ G127), N105 (= N128), F106 (≠ M129)
Sites not aligning to the query:
Query Sequence
>SMc02837 FitnessBrowser__Smeli:SMc02837
MNETDMKLVVVGAAGRMGQTLIRIIHETAGVRLHAAIERTGSPFIGRDAGELAGAGPMGV
AVTDKPLEAFVEAEGVLDFTAPAATVEFAGLAAQARIVHVVGTTGCSADDEARIRAAARH
ARVIKSGNMSLGVNLLGVLTEKAARALPAGGWDIEILEMHHKHKVDAPSGTALLLGEAAA
RGRGIDLADHSVRVRDGHTGARPEGSIGFATLRGGSVIGEHSVVIAGEGEMVTLSHSATD
RSIFARGAVAAALWGRSRKPGFYSMLDVLGLD
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory