SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SMc02864 FitnessBrowser__Smeli:SMc02864 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
47% identity, 96% coverage: 7:250/254 of query aligns to 64:300/453 of Q9VLJ8

query
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Q9VLJ8

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Sites not aligning to the query:

62 modified: Phosphothreonine

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
54% identity, 96% coverage: 10:254/254 of query aligns to 6:248/271 of Q72J02

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Q72J02

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C192 (= C198) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.

Sites not aligning to the query:

268 C→S: Still able to form a thioester complex with TtuB.

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
52% identity, 96% coverage: 6:250/254 of query aligns to 4:240/270 of D4GSF3

query
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D4GSF3

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C188 (= C198) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
46% identity, 94% coverage: 11:250/254 of query aligns to 65:309/482 of O59954

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G82 (= G28) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G46) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R76) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (≠ G131) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (≠ D161) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (= G209) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
44% identity, 95% coverage: 2:243/254 of query aligns to 34:272/440 of P38820

query
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P38820

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F

L

L

L

M

L

L

Y

Y

D

S

A

G

L

F

A

P

A

Q

Q

Q

C225 (= C198) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.

Sites not aligning to the query:

397 mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
51% identity, 94% coverage: 5:244/254 of query aligns to 53:285/460 of O95396

query
sites
O95396

A

A

T

A

L

L

A

S

P

R

S

D

E

E

I

I

A

L

R

R

Y

Y

A

S

R

R

H

Q

I

L

V

V

L

L

P

P

E

E

I

L

G

G

G

V

P

H

G

G

Q

Q

Q

L

K

R

L

L

K

G

A

T

A

A

R

C

V

V

L

L

V

I

I

V

G

G

A

C

G

G

L

L

G

G

A

C

P

P

A

L

L

A

Q

Q

Y

Y

L

L

A

A

G

G

V

V

G

G

T

R

L

L

G

G

I

L

V

V

D

D

D

Y

D

D

V

V

S

E

L

M

S

S

N

N

L

L

Q

A

R

R

Q

Q

V

V

I

L

H

H

A

G

T

E

A

A

D

L

I

A

G

G

R

Q

P

A

K

K

V

A

E

F

S

S

A

A

A

S

I

L

D

R

A

R

L

L

N

N

P

S

H

A

V

V

K

E

V

C

V

V

A

P

H

Y

P
x
T

V
x
Q

R
x
A

L
|
L

G
x
T

P
|
P

A
|
A

N
x
T

A
|
A

D
x
L

A
x
D

L
|
L

F
x
V

R
|
R

Q
x
R

Y
|
Y

D
|
D

L
x
V

V
|
V

V
x
A

D
|
D

G
x
C

S
|
S

D
|
D

N
|
N

F
x
V

D
x
P

T
|
T

R
|
R

Y
|
Y

L
|
L

A
x
V

A
x
N

D
|
D

T
x
A

A
x
C

E
x
V

A
x
L

A
|
A

G
|
G

V
x
R

A
x
P

L
|
L

V
|
V

T
x
S

G
x
A

A
x
S

V
x
A

G
x
L

R
|
R

F
|
F

D
x
E

G
|
G

S
x
Q

V
x
I

T
|
T

V
|
V

L

-

K

-

P

-

Y
|
Y

G
x
H

T
x
Y

D
|
D

A
x
G

E

-

G

-

H

-

P

-

N
x
G

P
|
P

S
x
C

Y
|
Y

R
|
R

D
x
C

L
x
I

F
|
F

P
|
P

E
x
Q

P
|
P

P
x
A

G
x
E

T
|
T

V
|
V

P
x
T

T
x
N

C
|
C

A

A

E

D

A

G

G

G

V

V

L

L

G

G

A

V

L

V

T

T

G

G

V

V

I

L

G

G

T

C

L

L

Q

Q

A

A

M

L

E

E

A

V

I

L

K

K

L

I

V

A

T

A

G

G

I

L

G

G

E

P

P

S

L

Y

I

S

G

G

R

S

L

L

Y

F

D

D

A

A

L

L

A

R

A

G

Q

H

F

F

158:238 (vs. 110:197, 44% identical) Interaction with NFS1
C239 (= C198) mutation to A: Impairs sulfurtransferase activity.

Sites not aligning to the query:

316 modified: Disulfide link with 324; C→A: Does not affect sulfurtransferase activity.
324 modified: Disulfide link with 316; C→A: Does not affect sulfurtransferase activity.
365 C→A: Does not affect sulfurtransferase activity.
412 active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; C→A: Abolishes sulfurtransferase activity.
413 K→R: Does not affect sulfurtransferase specificity and activity.
414 L→K: Does not affect sulfurtransferase specificity and activity.
415 G→A: Does not affect sulfurtransferase specificity and activity.
416 N→V: Does not affect sulfurtransferase specificity and activity.
417 D→R: Results in 470-fold increased activity.; D→T: Results in 90-fold increased activity.
458 P→G: Does not affect sulfurtransferase specificity and activity.
460 Y→A: Does not affect sulfurtransferase specificity and activity.

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
45% identity, 97% coverage: 5:251/254 of query aligns to 2:240/249 of P12282

query
sites
P12282

A

A

T

E

L

L

A

S

P

D

S

Q

E

E

I

M

A

L

R

R

Y

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

P

R

E

G

I

F

G

D

G

F

P

D

G

G

Q

Q

Q

E

K

A

L

L

K

K

A

D

A

S

R

R

V

V

L

L

V

I

I

V

G

G

A

L

G

G

L

L

G

G

A
x
C

P

A

A

A

L

S

Q

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

L

L

G

T

I

L

V

L

D

D

D

F

D

D

V

T

V

V

S

S

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q

Q

V

T

I

L

H

H

A

S

T

D

A

A

D

T

I

V

G

G

R

Q

P

P

K

K

V

V

E

E

S

S

A

A

A

R

A

D

A

A

I

L

D

T

A

R

L

I

N

N

P

P

H

H

V

I

K

A

V

I

V

T

A

P

H

V

P

N

V

A

R

L

L

L

G

D

P

D

A

A

N

E

A

L

D

A

A

A

L

L

F

I

R

A

Q

E

Y

H

D

D

L

L

V

V

V

L

D

D

G
x
C

S

T

D
|
D

N

N

F

V

D

A

T

V

R

R

Y

N

L

Q

A

L

A

N

D

A

T

G

A
x
C

E

F

A

A

G

K

V

V

A

P

L

L

V

V

T

S

G

G

A

A

V

A

G

I

R

R

F

M

D

E

G

G

S

Q

V

I

T

T

V

V

L

F

K

-

P

-

Y

-

G

-

T

T

D

Y

A

Q

E

D

G

G

H

E

P

-

N

-

P

P

S
x
C

Y

Y

R

R

-
x
C

-

L

-

S

D

R

L

L

F

F

P

G

E

E

P

-

G

-

T

N

V

A

P

L

T

T

C
|
C

A

V

E

E

A

A

G

G

V

V

L

M

G

A

A

P

L

L

T

I

G

G

V

V

I

I

G

G

T

S

L

L

Q

Q

A

A

M

M

E

E

A

A

I

I

K

K

L

M

V

L

T

A

G

G

I

Y

G

G

E

K

P

P

L

A

I

S

G

G

R

K

L

I

L

V

L

M

Y

Y

D

D

A

A

L

M

A

T

A
x
C

Q

Q

F

F

N

R

T

E

I

M

R

K

Y

L

R

M

R

R

R14 (= R17) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (≠ A47) mutation to A: No effect.
R73 (= R76) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (≠ G131) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D133) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (≠ A145) mutation to A: No effect.
C172 (≠ S181) mutation to A: No zinc bound and no enzyme activity.
C175 (vs. gap) mutation to A: No zinc bound and no enzyme activity.
C187 (= C198) mutation to A: No effect.
C231 (≠ A242) mutation to A: No effect.

Sites not aligning to the query:

244 C→A: No zinc bound and almost no enzyme activity.
247 C→A: No zinc bound and almost no enzyme activity.

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
44% identity, 97% coverage: 5:251/254 of query aligns to 1:232/240 of 1jwbB

query
sites
1jwbB

A

A

T

E

L

L

A

S

P

D

S

Q

E

E

I

M

A

L

R

R

Y

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

P

R

E

G

I

F

G

D

G

F

P

D

G

G

Q

Q

Q

E

K

A

L

L

K

K

A

D

A

S

R

R

V

V

L

L

V

I

I

V

G
|
G

A

L

G
|
G

L

L

G

G

A

C

P

A

A

A

L

S

Q

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

L

L

G

T

I

L

V

L

D
|
D

D
x
F

D

D

V

T

V

V

S

S

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

T

I

L

H

H

A

S

T

D

A

A

D

T

I

V

G

G

R

Q

P

P

K
|
K

V

V

E

E

S

S

A

A

A

R

A

D

A

A

I

L

D

T

A

R

L

I

N

N

P

P

H

H

V

I

K

A

V

I

V

T

A

P

H

V

P

N

V

A

R
x
L

L

L

G

D

P

D

A

A

N

E

A

L

D

A

A

A

L

L

F

I

R

A

Q

E

Y

H

D

D

L

L

V

V

V

L

D

D

G
x
C

S
x
T

D
|
D

N
|
N

F

V

D

A

T
x
V

R

R

Y

N

L

Q

A

L

A

N

D

A

T

G

A

C

E

F

A

A

G

K

V

V

A

P

L

L

V

V

T

S

G

G

A

A

V

A

G

I

R

R

F

M

D

E

G

G

S

Q

V

I

T

T

V

V

L

F

K

-

P

-

Y

-

G

-

T

T

D

Y

A

Q

E

D

G

G

H

E

P

-

N

-

P

P

S
x
C

Y

Y

R

R

D

C

L

L

F

-

P

-

E

-

P

-

G

-

T

-

V

S

P

R

T

L

C

F

A

G

E

E

A

A

G

G

V

V

L

M

G

A

A

P

L

L

T

I

G

G

V

V

I

I

G

G

T

S

L

L

Q

Q

A

A

M

M

E

E

A

A

I

I

K

K

L

M

V

L

T

A

G

G

I

Y

G

G

E

K

P

P

L

A

I

S

G

G

R

K

L

I

L

V

L

M

Y

Y

D

D

A

A

L

M

A

T

A

C

Q

Q

F

F

N

R

T

E

I

M

R

K

Y

L

R

M

R

R

active site: R13 (= R17), D129 (= D133)
binding adenosine monophosphate: G37 (= G41), G39 (= G43), G40 (= G44), D61 (= D65), F62 (≠ D66), K85 (= K89), L108 (≠ R112), C127 (≠ G131), T128 (≠ S132), D129 (= D133), N130 (= N134), V133 (≠ T137)
binding zinc ion: C171 (≠ S181)

Sites not aligning to the query:

binding zinc ion: 236, 239

1jw9B Structure of the native moeb-moad protein complex (see paper)
44% identity, 97% coverage: 5:251/254 of query aligns to 1:232/240 of 1jw9B

query
sites
1jw9B

A

A

T

E

L

L

A

S

P

D

S

Q

E

E

I

M

A

L

R

R

Y

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

P

R

E

G

I

F

G

D

G

F

P

D

G

G

Q

Q

Q

E

K

A

L

L

K

K

A

D

A

S

R

R

V

V

L

L

V

I

I

V

G

G

A

L

G

G

L

L

G

G

A

C

P

A

A

A

L

S

Q

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

L

L

G

T

I

L

V

L

D

D

D

F

D

D

V

T

V

V

S

S

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

T

I

L

H

H

A

S

T

D

A

A

D

T

I

V

G

G

R

Q

P

P

K

K

V

V

E

E

S

S

A

A

A

R

A

D

A

A

I

L

D

T

A

R

L

I

N

N

P

P

H

H

V

I

K

A

V

I

V

T

A

P

H

V

P

N

V

A

R

L

L

L

G

D

P

D

A

A

N

E

A

L

D

A

A

A

L

L

F

I

R

A

Q

E

Y

H

D

D

L

L

V

V

V

L

D

D

G

C

S

T

D
|
D

N

N

F

V

D

A

T

V

R

R

Y

N

L

Q

A

L

A

N

D

A

T

G

A

C

E

F

A

A

G

K

V

V

A

P

L

L

V

V

T

S

G

G

A

A

V

A

G

I

R

R

F

M

D

E

G

G

S

Q

V

I

T

T

V

V

L

F

K

-

P

-

Y

-

G

-

T

T

D

Y

A

Q

E

D

G

G

H

E

P

-

N

-

P

P

S
x
C

Y

Y

R

R

D

C

L

L

F

-

P

-

E

-

P

-

G

-

T

-

V

S

P

R

T

L

C

F

A

G

E

E

A

A

G

G

V

V

L

M

G

A

A

P

L

L

T

I

G

G

V

V

I

I

G

G

T

S

L

L

Q

Q

A

A

M

M

E

E

A

A

I

I

K

K

L

M

V

L

T

A

G

G

I

Y

G

G

E

K

P

P

L

A

I

S

G

G

R

K

L

I

L

V

L

M

Y

Y

D

D

A

A

L

M

A

T

A

C

Q

Q

F

F

N

R

T

E

I

M

R

K

Y

L

R

M

R

R

active site: R13 (= R17), D129 (= D133)
binding zinc ion: C171 (≠ S181)

Sites not aligning to the query:

binding zinc ion: 236, 239

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
42% identity, 97% coverage: 5:251/254 of query aligns to 1:217/217 of 1jwaB

query
sites
1jwaB

A

A

T

E

L

L

A

S

P

D

S

Q

E

E

I

M

A

L

R

R

Y

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

P

R

E

G

I

F

G

D

G

F

P

D

G

G

Q

Q

Q

E

K

A

L

L

K

K

A

D

A

S

R

R

V

V

L

L

V

I

I

V

G

G

A

L

G
|
G

L

L

G

G

A

C

P

A

A

A

L

S

Q

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

L

L

G

T

I

L

V

L

D
|
D

D
x
F

D

D

V

T

V

V

S

S

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q

Q

V

T

I

L

H

H

A

S

T

D

A

A

D

T

I

V

G

G

R

Q

P

P

K
|
K

V

V

E

E

S

S

A

A

A

R

A

D

A

A

I

L

D

T

A

R

L

I

N

N

P

P

H

H

V

I

K

A

V

I

V

T

A

P

H

V

P

N

V

A

R
x
L

L

L

G

D

P

D

A

A

N

E

A

L

D

A

A

A

L

L

F

I

R

A

Q

E

Y

H

D

D

L

L

V

V

V

L

D

D

G

C

S

T

D
|
D

N
|
N

F

V

D

A

T
x
V

R

R

Y

N

L

Q

A

L

A

N

D

A

T

G

A

C

E

F

A

A

G

K

V

V

A

P

L

L

V

V

T

S

G

G

A

A

V

A

G

I

R

R

F

M

D

E

G

G

S

Q

V

I

T

T

V

V

L

F

K

-

P

-

Y

-

G

-

T

-

D

-

A

-

E

-

G

-

H

-

P

-

N

-

P

-

S

T

Y

Y

R

-

D

-

L

-

F

-

P

-

E

-

P

-

G

-

T

-

V

-

P

-

T

-

C

-

A

-

E

E

A

A

G

G

V

V

L

M

G

A

A

P

L

L

T

I

G

G

V

V

I

I

G

G

T

S

L

L

Q

Q

A

A

M

M

E

E

A

A

I

I

K

K

L

M

V

L

T

A

G

G

I

Y

G

G

E

K

P

P

L

A

I

S

G

G

R

K

L

I

L

V

L

M

Y

Y

D

D

A

A

L

M

A

T

A

C

Q

Q

F

F

N

R

T

E

I

M

R

K

Y

L

R

M

R

R

active site: R13 (= R17), D129 (= D133)
binding adenosine-5'-triphosphate: G39 (= G43), G40 (= G44), D61 (= D65), F62 (≠ D66), R72 (= R76), K85 (= K89), L108 (≠ R112), D129 (= D133), N130 (= N134), V133 (≠ T137)

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
41% identity, 87% coverage: 2:223/254 of query aligns to 1:213/423 of 6yubA

query
sites
6yubA

T

S

T

T

D

Q

A

K

T

S

L

L

A

S

P

K

S

E

E

E

I

I

A

E

R

R

Y

Y

A

S

R

R

H

Q

I

M

V

I

L

V

P

P

E

G

I

M

G

G

G

K

P

E

G

G

Q

Q

Q

L

K

R

L

L

K

M

A

N

A

A

R

K

V

V

L

L

V

I

I

I

G

G

A

A

G

G

L

L

G

G

A

C

P

P

A

A

L

A

Q

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

I

G

G

I

I

V

V

D

D

D

G

D

D

V

S

V

V

S

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

I

A

H

H

A

A

T

T

A

K

D

R

I

V

G

G

R

M

P

L

K

K

V

V

E

D

S

S

A

L

A

I

A

T

A

H

I

L

D

I

A

E

L

I

N

N

P

P

H

L

V

P

K

V

V

Y

V

V

A

P

H

Y

P

R

V

F

R

D

L

L

G

T

P

P

A

Q

N

N

A

A

D

A

A

Q

L

I

F

I

R

K

Q

P

Y

W

D

D

L

V

V

I

V

L

D

D

G

C

S

T

D

D

N

N

F

P

D

A

T

T

R

R

Y

Y

L

L

A

I

A

S

D

D

T

V

A

C

E

V

A

L

G

G

V

K

A

P

L

L

V

V

T

S

G

A

A

A

V

S

G

V

R

Q

F

K

D

S

G

G

S

Q

V

L

T

I

V

V

L

L

K

N

-

C

-

P

-

T

P

P

Y

Q

G

G

T

V

D

-

A

V

E

N

G

K

H

K

P

A

N

A

P

P

S
x
C

Y

Y

R

R

D
x
C

L

C

F

F

P

K

E

K

P

P

P

-

G

-

T

-

V

-

P

-

T

-

C

-

A

-

E

-

A

-

G

G

V

I

L

M

G

G

A

P

L

V

T

V

G

G

V

M

I

M

G

G

T

V

L

A

Q

Q

A

A

M

G

E

E

A

A

I

I

K

K

L

I

V

L

binding zinc ion: C183 (≠ S181), C186 (≠ D184)

Sites not aligning to the query:

binding zinc ion: 266, 269

1zfnA Structural analysis of escherichia coli thif (see paper)
43% identity, 96% coverage: 11:253/254 of query aligns to 5:238/244 of 1zfnA

query
sites
1zfnA

E

D

I

F

A

M

R

R

Y

Y

A

S

R
|
R

H

Q

I

I

V

L

L

L

P

D

E

D

I

I

G

A

G

L

P

D

G

G

Q

Q

K

K

L

L

K

L

A

D

A

S

R

Q

V

V

L

L

V

I

I
|
I

G
|
G

A

L

G
|
G

L

L

G

G

A

T

P

P

A

A

L

A

Q

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

L

G

V

I

L

V

A

D
|
D

D

D

V

D

V

V

S

H

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q
|
Q

V

I

I

L

H

F

A

T

T

T

A

E

D

D

I

I

G

D

R

R

P

P

K
|
K

V

S

E

Q

S

V

A

S

A

Q

A

R

I

L

D

T

A

Q

L

L

N

N

P

P

H

D

V

I

K

Q

V

L

V

T

A

A

H

L

P

Q

V

Q

R

R

L

L

-

T

G

G

P

E

A

A

N

L

A

K

D

D

A

A

L

V

F

A

R

R

Q

-

Y

A

D

D

L

V

V

V

V

L

D

D

G

C

S
x
T

D
|
D

N

N

F

M

D

A

T
|
T

R

R

Y

Q

L

E

A

I

A

N

D

A

T

A

A

C

E

V

A

A

A

L

G

N

V

T

A

P

L

L

V

I

T

T

G

A

A

S

V

A

G

V

R

G

F

F

D

G

G

G

S

Q

V

L

T

M

V

V

L

L

K

T

P

P

Y

-

G

-

T

-

D

-

A

-

E

-

G

P

H

W

P

E

N

Q

P

G

S
x
C

Y

Y

R

R

D
x
C

L

L

F

W

P

P

E

D

P

N

P

Q

P

E

P

P

G

E

T

R

V

-

P

-

T

N

C

C

A

R

E

T

A

A

G

G

V

V

L

V

G

G

A

P

L

V

T

V

G

G

V

V

I

M

G

G

T

T

L

L

Q

Q

A

A

M

L

E

E

A

A

I

I

K

K

L

L

V

L

T

S

G

G

I

I

G

E

E

T

P

P

L

-

I

A

G

G

R

E

L

L

L

R

L

L

Y

F

D

D

A

G

L

K

A

S

Q

Q

F

W

N

R

T

S

I

L

R

A

Y

L

R

R

G

A

T

S

active site: R11 (= R17), D127 (= D133)
binding adenosine-5'-triphosphate: I34 (= I40), G35 (= G41), G37 (= G43), G38 (= G44), D59 (= D65), R70 (= R76), Q71 (= Q77), K83 (= K89), T126 (≠ S132), D127 (= D133), T131 (= T137)
binding zinc ion: C169 (≠ S181), C172 (≠ D184)

Sites not aligning to the query:

binding zinc ion: 240, 243

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
43% identity, 96% coverage: 11:253/254 of query aligns to 5:238/251 of P30138

query
sites
P30138

E

D

I

F

A

M

R

R

Y

Y

A

S

R

R

H

Q

I

I

V

L

L

L

P

D

E

D

I

I

G

A

G

L

P

D

G

G

Q

Q

K

K

L

L

K

L

A

D

A

S

R

Q

V

V

L

L

V

I

I

I

G

G

A

L

G

G

L

L

G

G

A

T

P

P

A

A

L

A

Q

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

L

G

V

I

L

V

A

D

D

V

D

V

V

S

H

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

I

I

L

H

F

A

T

T

T

A

E

D

D

I

I

G

D

R

R

P

P

K

K

V

S

E

Q

S

V

A

S

A

Q

A

R

I

L

D

T

A

Q

L

L

N

N

P

P

H

D

V

I

K

Q

V

L

V

T

A

A

H

L

P

Q

V

Q

R

R

L

L

-

T

G

G

P

E

A

A

N

L

A

K

D

D

A

A

L

V

F

A

R

R

Q

-

Y

A

D

D

L

V

V

V

V

L

D

D

G

C

S

T

D

D

N

N

F

M

D

A

T

T

R

R

Y

Q

L

E

A

I

A

N

D

A

T

A

A

C

E

V

A

A

A

L

G

N

V

T

A

P

L

L

V

I

T

T

G

A

A

S

V

A

G

V

R

G

F

F

D

G

G

G

S

Q

V

L

T

M

V

V

L

L

K

T

P

P

Y

P

G

W

T

E

D

Q

A

G

E

-

G

-

H

-

P

-

N

-

P

-

S
x
C

Y

Y

R

R

D
x
C

L

L

F
x
W

P

P

E

D

P

N

P

Q

P

E

P

P

G

E

T

R

V

-

P

-

T

N

C
|
C

A

R

E

T

A

A

G

G

V

V

L

V

G

G

A

P

L

V

T

V

G

G

V

V

I

M

G

G

T

T

L

L

Q

Q

A

A

M

L

E

E

A

A

I

I

K

K

L

L

V

L

T

S

G

G

I

I

G

E

E

T

P

P

L

-

I

A

G

G

R

E

L

L

L

R

L

L

Y

F

D

D

A

G

L

K

A

S

Q

Q

F

W

N

R

T

S

I

L

R

A

Y

L

R

R

G

A

T

S

C169 (≠ S181) binding
C172 (≠ D184) binding
W174 (≠ F186) mutation to A: No adenylation of ThiS.
C184 (= C198) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.

Sites not aligning to the query:

240 binding
243 binding

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
40% identity, 87% coverage: 2:223/254 of query aligns to 2:212/289 of 6yubB

query
sites
6yubB

T

S

T

T

D

Q

A

K

T

S

L

L

A

S

P

K

S

E

E

E

I

I

A

E

R

R

Y

Y

A

S

R

R

H

Q

I

M

V

I

L

V

P

P

E

G

I

M

G

G

G

K

P

E

G

G

Q

Q

Q

L

K

R

L

L

K

M

A

N

A

A

R

K

V

V

L

L

V

I

I

I

G

G

A

A

G

G

L

L

G

G

A

C

P

P

A

A

L

A

Q

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

I

G

G

I

I

V

V

D

D

D

G

D

D

V

S

V

V

S

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

I

A

H

H

A

A

T

T

A

K

D

R

I

V

G

G

R

M

P

L

K

K

V

V

E

D

S

S

A

L

A

I

A

T

A

H

I

L

D

I

A

E

L

I

N

N

P

P

H

L

V

P

K

V

V

Y

V

V

A

P

H

Y

P

R

V

F

R

D

L

L

G

T

P

P

A

Q

N

N

A

A

D

A

A

Q

L

I

F

I

R

K

Q

P

Y

W

D

D

L

V

V

I

V

L

D

D

G

C

S

T

D

D

N

N

F

P

D

A

T

T

R

R

Y

Y

L

L

A

I

A

S

D

D

T

V

A

C

E

V

A

L

G

G

V

K

A

P

L

L

V

V

T

S

G

A

A

A

V

S

G

V

R

Q

F

K

D

S

G

G

S

Q

V

L

T

I

V

V

L

L

K

N

-

C

-

P

-

T

P

P

Y

Q

G

G

T

V

D

-

A

V

E

N

G

K

H

K

P

A

N

A

P

P

S
x
C

Y

Y

R

R

D

-

L

-

F

-

P

-

E

-

P

-

G

-

T

-

V

-

P

-

T

-

C
|
C

A

C

E

F

A

K

G

G

V

I

L

M

G

G

A

P

L

V

T

V

G

G

V

M

I

M

G

G

T

V

L

A

Q

Q

A

A

M

G

E

E

A

A

I

I

K

K

L

I

V

L

binding zinc ion: C184 (≠ S181), C187 (= C198)

Sites not aligning to the query:

binding zinc ion: 265

1zud3 Structure of this-thif protein complex (see paper)
43% identity, 96% coverage: 11:253/254 of query aligns to 5:233/240 of 1zud3

query
sites
1zud3

E

D

I

F

A

M

R

R

Y

Y

A

S

R
|
R

H

Q

I

I

V
x
L

L

L

P
x
D

E

D

I

I

G

A

G

L

P

D

G

G

Q

Q

K

K

L

L

K

L

A

D

A

S

R

Q

V

V

L

L

V

I

I

I

G

G

A

L

G

G

L

L

G

G

A

T

P

P

A

A

L

A

Q

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

L

G

V

I

L

V

A

D

D

V

D

V

V

S

H

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

I

I

L

H

F

A

T

T

T

A

E

D

D

I

I

G

D

R

R

P

P

K

K

V

S

E

Q

S

V

A

S

A

Q

A

R

I

L

D

T

A

Q

L

L

N

N

P

P

H

D

V

I

K

Q

V

L

V

T

A

A

H

L

P

Q

V

Q

R

R

L

L

-

T

G

G

P

E

A

A

N

L

A

K

D

D

A

A

L

V

F

A

R

R

Q

-

Y

A

D

D

L

V

V

V

V

L

D

D

G

C

S

T

D
|
D

N

N

F

M

D

A

T

T

R

R

Y

Q

L

E

A

I

A

N

D

A

T

A

A

C

E

V

A

A

A

L

G

N

V

T

A

P

L

L

V

I

T

T

G

A

A

S

V

A

G

V

R

G

F

F

D

G

G

G

S

Q

V

L

T

M

V

V

L

L

K

T

P

P

Y

-

G

-

T

-

D

-

A

-

E

-

G

P

H

W

P

E

N

Q

P

G

S
x
C

Y

Y

R

R

D

C

L

L

F

W

P

P

E

D

P

N

P

Q

P

E

P

P

G

-

T

-

V

-

P

-

T

-

C

-

A

-

E

T

A

A

G

G

V

V

L

V

G

G

A

P

L

V

T

V

G

G

V

V

I

M

G

G

T

T

L

L

Q

Q

A

A

M

L

E

E

A

A

I

I

K

K

L

L

V

L

T

S

G

G

I

I

G

E

E

T

P

P

L

-

I

A

G

G

R

E

L

L

L

R

L

L

Y

F

D

D

A

G

L

K

A

S

Q

Q

F

W

N

R

T

S

I

L

R

A

Y

L

R

R

G

A

T

S

active site: R11 (= R17), D127 (= D133)
binding calcium ion: L14 (≠ V20), D16 (≠ P22)
binding zinc ion: C169 (≠ S181)

Sites not aligning to the query:

binding zinc ion: 235, 238

O42939 Ubiquitin-activating enzyme E1-like; Pmt3-activating enzyme subunit 2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
34% identity, 67% coverage: 30:198/254 of query aligns to 21:181/628 of O42939

query
sites
O42939

Q

R

K

N

L

F

K

K

A

S

A

A

R

K

V

V

L

L

V

L

I

V

G

G

A

A

G

G

L

I

G

G

A

C

P

E

A

L

L

L

Q

K

Y

N

L

L

A

L

A

M

A

S

G

G

V

V

G

K

T

E

L

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G

H

I

I

V

I

D

D

D

L

D

D

V

T

V

I

S

D

L

L

S

S

N

N

L

L

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N

R

R

Q

Q

V

F

I

L

H

F

A

R

T

K

A

K

D

H

I

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G

K

R

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P

K

K

V

A

E

I

S

V

A

A

A

K

A

T

I

A

D

S

A

S

L

F

N

N

P

P

H

N

V

V

K

K

V

L

V

E

A

A

H

Y

P

H

V

A

R

N

L

I

G

K

P

E

A

D

N

R

A

F

D

N

-

V

A

A

L

W

F

F

R

R

Q

Q

Y

F

D

D

L

L

V

V

V

F

D

N

G

A

S

L

D

D

N

N

F

L

D

D

T

A

R

R

Y

R

L

H

A

V

A

N

D

K

T

Q

A

C

E

L

A

L

A

A

G

S

V

V

A

P

L

L

V

I

T

E

G

S

A

G

V

T

G

T

R

G

F

F

D

L

G

G

S

Q

V

V

T

Q

V

V

L

I

K

-

P

-

Y

-

G

-

T

-

D

-

A

-

E

-

G

I

H

H

P

G

N

K

P

T

S

E

Y

C

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Y

D

D

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C

F

N

P

P

E

K

P

-

P

E

P

P

G

K

T

T

V

Y

P

P

T

V

C

C

Sites not aligning to the query:

559 modified: Phosphoserine
563 modified: Phosphoserine

3kydB Human sumo e1~sumo1-amp tetrahedral intermediate mimic (see paper)
30% identity, 81% coverage: 36:240/254 of query aligns to 16:227/477 of 3kydB

query
sites
3kydB

R

R

V

V

L

L

V

V

I

V

G

G

A

A

G

G

L
x
I

G

G

A

C

P

E

A

L

L

L

Q

K

Y

N

L

L

A

V

A

L

A

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G

G

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F

G

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T

H

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I

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I

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|
D

D
x
L

D

D

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T

V

I

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D

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S

S

N

N

L

L

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N

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A

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A

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G

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K

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A

A

A

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A

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A

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A

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F

N

Y

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P

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K

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A

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N

V

I

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A

A

-

Y

H

H

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x
S

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x
I

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N

P

P

A

D

N

Y

A

N

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A

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F

F

F

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R

Q

Q

Y

F

D

I

L

L

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V

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N

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x
L

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D

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N

F

R

D

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A

R

R

Y

N

L

H

A

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A

N

D

R

T

M

A

C

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A

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D

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A

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T

T

V

T

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I

K

K

P

K

Y

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G

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T

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E

A
x
C

-

Y

E

E

G
x
C

H

H

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K

P

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S

T

Y

Q

R

R

D

-

L

T

F

F

P

P

-

G

-
x
C

-
x
T

-

I

-
x
R

-

N

E

T

P

P

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S

P

E

P

P

G

I

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C

P

I

T

V

C

W

A

A

E

K

A

Y

G

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F

L

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G

Q

A

L

L

F

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T

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K

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E

I

W

G

A

T

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Q

T

A

G

M

Y

E

D

A

P

I

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K

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T

T

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K

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I

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Y

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L

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Y

M

D

D

A

K

L

L

active site: D114 (= D133), C170 (vs. gap), T171 (vs. gap), R173 (vs. gap)
binding 5'-{[(3-aminopropyl)sulfonyl]amino}-5'-deoxyadenosine: I25 (≠ L45), D45 (= D65), L46 (≠ D66), K69 (= K89), S92 (≠ V111), I93 (≠ R112), L113 (≠ S132), D114 (= D133), N115 (= N134), C170 (vs. gap), R173 (vs. gap)
binding zinc ion: C155 (≠ A174), C158 (≠ G176)

Sites not aligning to the query:

binding zinc ion: 370, 373

3kycB Human sumo e1 complex with a sumo1-amp mimic (see paper)
34% identity, 60% coverage: 36:187/254 of query aligns to 15:167/548 of 3kycB

query
sites
3kycB

R

R

V

V

L

L

V

V

I

V

G

G

A

A

G
|
G

L

I

G

G

A

C

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E

A

L

L

L

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Y

N

L

L

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A

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D

D
x
L

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D

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T

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D

L

V

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S

N

N

L

L

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N

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A

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K

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A

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A

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A

S

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L

A

Q

L

F

N

Y

P

P

H

K

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K

N

V

I

V

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A

A

-

Y

H

H

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x
S

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x
I

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M

G

N

P

P

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D

N

Y

A

N

D

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A

E

L

F

F

F

R

R

Q

Q

Y

F

D

I

L

L

V

V

V

M

D

N

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x
L

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|
D

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|
N

F

R

D

A

T

A

R

R

Y

N

L

H

A

V

A

N

D

R

T

M

A

C

E

L

A

A

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D

V

V

A

P

L

L

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I

T

E

G

S

A

G

V

T

G

A

R

G

F

Y

D

L

G

G

S

Q

V

V

T

T

V

T

L

I

K

K

P

K

Y

G

G

V

T

T

D

E

A
x
C

-

Y

E

E

G
x
C

H

H

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P

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P

P

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T

Y

Q

R

R

D

-

L

T

F

F

P

P

active site: D113 (= D133)
binding 5'-deoxy-5'-(sulfamoylamino)adenosine: G22 (= G43), G23 (= G44), D44 (= D65), L45 (≠ D66), K68 (= K89), S91 (≠ V111), I92 (≠ R112), L112 (≠ S132), D113 (= D133), N114 (= N134)
binding zinc ion: C154 (≠ A174), C157 (≠ G176)

Sites not aligning to the query:

active site: 169, 170, 172
binding zinc ion: 407, 410

1y8qB Sumo e1 activating enzyme sae1-sae2-mg-atp complex (see paper)
34% identity, 60% coverage: 36:187/254 of query aligns to 14:166/510 of 1y8qB

query
sites
1y8qB

R

R

V

V

L

L

V

V

I

V

G

G

A

A

G

G

L

I

G

G

A

C

P

E

A

L

L

L

Q

K

Y

N

L

L

A

V

A

L

A

T

G

G

V

F

G

S

T

H

L

I

G

D

I

L

V

I

D
|
D

D
x
L

D

D

V

T

V

I

S

D

L

V

S

S

N

N

L

L

Q

N

R

R

Q

Q

V

F

I

L

H

F

A

Q

T

K

A

K

D

H

I

V

G

G

R

R

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K
|
K

V

A

E

Q

S

V

A

A

A

K

A

E

A

S

I

V

D

L

A

Q

L

F

N

Y

P

P

H

K

V

A

K

N

V

I

V

V

A

A

-

Y

H

H

P

D

V
x
S

R
x
I

L

M

G

N

P

P

A

D

N

Y

A

N

D

V

A

E

L

F

F

F

R

R

Q

Q

Y

F

D

I

L

L

V

V

V

M

D

N

G

A

S
x
L

D
|
D

N
|
N

F

R

D

A

T

A

R

R

Y

N

L

H

A

V

A

N

D

R

T

M

A

C

E

L

A

A

G

D

V

V

A

P

L

L

V

I

T

E

G

S

A

G

V

T

G

A

R

G

F

Y

D

L

G

G

S

Q

V

V

T

T

V

T

L

I

K

K

P

K

Y

G

G

V

T

T

D

E

A
x
C

-

Y

E

E

G
x
C

H

H

P

P

N

K

P

P

S

T

Y

Q

R

R

D

-

L

T

F

F

P

P

active site: D112 (= D133)
binding adenosine-5'-triphosphate: D43 (= D65), L44 (≠ D66), K67 (= K89), S90 (≠ V111), I91 (≠ R112), L111 (≠ S132), D112 (= D133), N113 (= N134)
binding zinc ion: C153 (≠ A174), C156 (≠ G176)

Sites not aligning to the query:

6xogB Structure of sumo1-ml786519 adduct bound to sae (see paper)
33% identity, 58% coverage: 36:182/254 of query aligns to 12:160/500 of 6xogB

query
sites
6xogB

R

R

V

V

L

L

V

V

I

V

G

G

A

A

G

G

L

I

G

G

A

C

P

E

A

L

L

L

Q

K

Y

N

L

L

A

V

A

L

A

T

G

G

V

F

G

S

T

H

L

I

G

D

I

L

V

I

D
|
D

D
x
L

D

D

V

T

V

I

S

D

L

V

S

S

N

N

L

L

Q

N

R

R

Q

Q

V

F

I

L

H

F

A

Q

T

K

A

K

D

H

I

V

G

G

R

R

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S

K
|
K

V

A

E

Q

S

V

A

A

A

K

A

E

A

S

I

V

D

L

A

Q

L

F

N

Y

P

P

H

K

V

A

K

N

V

I

V

V

A

A

-

Y

H

H

P

D

V
x
S

R
x
I

L
x
M

G

N

P

P

A

D

N

Y

A

N

D

V

A

E

L

F

F

F

R

R

Q

Q

Y

F

D

I

L

L

V

V

V

M

D

N

G
x
A

S

L

D
|
D

N
|
N

F

R

D

A

T

A

R

R

Y

N

L

H

A

V

A

N

D

R

T

M

A

C

E

L

A

A

G

D

V

V

A

P

L

L

V

I

T

E

G

S

A

G

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T

G

A

R

G

F

Y

D

L

G

G

S

Q

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T

T

V

T

L

I

K

K

P

K

Y

G

G

V

T

T

D

E

A
x
C

-

Y

E

E

G
x
C

H

H

P

P

N

K

P

P

S

T

Y

F

active site: D110 (= D133)
binding {(1R,2R,3S,4R)-4-[(5-{4-[(1S)-1-(6-bromopyridin-2-yl)-1-hydroxyethyl]thiophene-2-carbonyl}pyrimidin-4-yl)amino]-2,3-dihydroxycyclopentyl}methyl sulfamate: D41 (= D65), L42 (≠ D66), K65 (= K89), S88 (≠ V111), I89 (≠ R112), M90 (≠ L113), A108 (≠ G131), D110 (= D133), N111 (= N134)
binding zinc ion: C151 (≠ A174), C154 (≠ G176)

Sites not aligning to the query:

active site: 163, 164, 166
binding zinc ion: 398, 401

Query Sequence

>SMc02864 FitnessBrowser__Smeli:SMc02864
MTTDATLAPSEIARYARHIVLPEIGGPGQQKLKAARVLVIGAGGLGAPALQYLAAAGVGT
LGIVDDDVVSLSNLQRQVIHATADIGRPKVESAAAAIDALNPHVKVVAHPVRLGPANADA
LFRQYDLVVDGSDNFDTRYLAADTAEAAGVALVTGAVGRFDGSVTVLKPYGTDAEGHPNP
SYRDLFPEPPPPGTVPTCAEAGVLGALTGVIGTLQAMEAIKLVTGIGEPLIGRLLLYDAL
AAQFNTIRYRRGTR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory