SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SMc03204 SMc03204 dihydrolipoamide dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6cmzA 2.3 angstrom resolution crystal structure of dihydrolipoamide dehydrogenase from burkholderia cenocepacia in complex with fad and NAD
59% identity, 100% coverage: 2:464/464 of query aligns to 1:462/462 of 6cmzA

query
sites
6cmzA

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active site: C42 (= C43), C47 (= C48), S50 (= S51), Y184 (= Y186), E188 (= E190), H441 (= H443), E446 (= E448)
binding flavin-adenine dinucleotide: I10 (≠ L11), G11 (= G12), G12 (≠ A13), G13 (= G14), P14 (= P15), G15 (= G16), V33 (= V34), E34 (= E35), R35 (≠ K36), D36 (≠ A37), G40 (= G41), T41 (= T42), C42 (= C43), G46 (= G47), C47 (= C48), S50 (= S51), K51 (= K52), G115 (= G117), E116 (= E118), A117 (≠ G119), A144 (= A146), T145 (= T147), G146 (= G148), S147 (= S149), S164 (= S166), V180 (≠ I182), G181 (= G183), G183 (= G185), Y184 (= Y186), I185 (= I187), V203 (≠ L205), E204 (= E206), A205 (= A207), P211 (= P213), H236 (≠ A240), K237 (= K241), V238 (≠ R242), A267 (≠ T269), V268 (= V270), G269 (= G271), R270 (= R272), R273 (≠ V275), F277 (≠ W279), R289 (= R291), G308 (= G310), D309 (= D311), M315 (= M317), L316 (= L318), A317 (= A319), H318 (= H320), A320 (= A322), H441 (= H443), P442 (= P444)
binding flavin mononucleotide: L152 (= L154), P153 (= P155), S154 (≠ D156), V268 (= V270), R289 (= R291)
binding nicotinamide-adenine-dinucleotide: L232 (≠ V234), W233 (≠ F235), H236 (≠ A240), Q257 (≠ F259)

6cmzB 2.3 angstrom resolution crystal structure of dihydrolipoamide dehydrogenase from burkholderia cenocepacia in complex with fad and NAD
59% identity, 99% coverage: 2:461/464 of query aligns to 1:459/459 of 6cmzB

query
sites
6cmzB

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active site: C42 (= C43), C47 (= C48), S50 (= S51), Y184 (= Y186), E188 (= E190), H441 (= H443), E446 (= E448)
binding adenosine-5'-diphosphate: V150 (= V152), L152 (= L154), P153 (= P155), G181 (= G183), A182 (≠ G184), G183 (= G185), E204 (= E206), A205 (= A207), P211 (= P213), A267 (≠ T269), V268 (= V270), G269 (= G271)
binding flavin-adenine dinucleotide: I10 (≠ L11), G11 (= G12), G12 (≠ A13), G13 (= G14), P14 (= P15), G15 (= G16), V33 (= V34), E34 (= E35), R35 (≠ K36), D36 (≠ A37), G40 (= G41), T41 (= T42), C42 (= C43), I45 (≠ V46), G46 (= G47), C47 (= C48), S50 (= S51), K51 (= K52), G115 (= G117), E116 (= E118), A117 (≠ G119), A144 (= A146), T145 (= T147), G146 (= G148), S147 (= S149), S164 (= S166), Y184 (= Y186), I185 (= I187), R270 (= R272), R273 (≠ V275), F277 (≠ W279), G308 (= G310), D309 (= D311), M315 (= M317), L316 (= L318), A317 (= A319), H318 (= H320), A320 (= A322), H441 (= H443), P442 (= P444)

P09063 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val; EC 1.8.1.4 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see paper)
57% identity, 100% coverage: 2:464/464 of query aligns to 3:459/459 of P09063

query
sites
P09063

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A

R

K

K

L

L

G

G

S

A

K

Q

V

V

T

S

V

V

L

V

E
|
E

A

A

L

R

D

E

R

R

I

I

L

L

P

P

Q

T

Y

Y

D

D

A

S

D

E

L

L

S

T

K

A

P

P

V

V

M

A

K

E

R

S

L

L

G

K

E

K

L

L

G

G

V

I

E

A

V

L

F

H

T

L

R

-

T

-

A

-

A

G

K

H

R

S

L
x
V

S

E

A

G

D

Y

R

E

R

N

G

G

L

C

L

L

A

L

E

A

E

N

N

D

G

G

R

K

A

G

-

G

-

Q

F

L

E

R

V

L

P

E

A

A

E

D

K

R

V

V

L

L

V

V

T
x
A

V
|
V

G
|
G

R
|
R

R

R

P

P

V

R

T

T

D

K

G

G

W

F

G

N

L

L

E

E

E

C

I

L

D

D

L

L

D

K

H

M

S

N

G

G

R

A

F

A

I

I

R

A

I

I

D

D

D

E

Q

R

C

C

R

Q

T

T

S

S

M

M

R

H

G

N

V

V

Y

W

A

A

I

I

G

G

D
|
D

V

V

T

A

G

G

E

E

P

P

M
|
M

L

L

A
|
A

H

H

R

R

A

A

M

M

A

A

Q

Q

G

G

E

E

M

M

V

V

A

A

E

E

I

I

V

I

A

A

G

G

H

K

K

A

R

R

S

R

W

F

D

E

K

P

R

A

C

A

I

I

P

A

A

A

V

V

C

C

F

F

T

T

D

D

P

P

E

E

I

V

V

V

G

V

A

V

G

G

L

K

S

T

P

P

E

E

E

Q

A

A

R

S

A

Q

G

G

I

L

D

D

V

C

K

I

I

V

G

A

Q

Q

F

F

P

P

F

F

Q

A

A

A

N

N

G

G

R

R

A

A

M

M

T

S

T

L

L

E

S

S

E

K

D

S

G

G

F

F

V

V

R

R

V

V

I

V

A

A

R

R

A

R

D

D

N

N

H

H

L

L

V

I

L

L

G

G

I

W

Q

Q

A

A

V

V

G

G

H

V

G

A

V

V

S

S

E

E

L

L

S

S

A

T

T

A

F

F

A

A

L

Q

A

S

I

L

E

E

M

M

G

G

A

A

R

C

L

L

E

E

D

D

I

V

A

A

G

G

T

T

I

I

H

H

A

A

H

H

P

P

T

T

Q

L

S

G

E

E

A

A

F

V

Q

Q

E

E

A

A

L

L

K

R

T

A

L

L

G

G

H

H

A

A

L

L

H

H

I

I

36:44 (vs. 35:43, 56% identical) binding
C44 (= C43) modified: Disulfide link with 49, Redox-active
C49 (= C48) modified: Disulfide link with 44, Redox-active
K53 (= K52) binding
A119 (≠ G119) binding
A142 (= A146) binding
GGGYI 179:183 (= GGGYI 183:187) binding
Y182 (= Y186) binding
E202 (= E206) binding
V236 (≠ L243) binding
AVGR 264:267 (≠ TVGR 269:272) binding
D306 (= D311) binding
M312 (= M317) binding
A314 (= A319) binding

1lvlA The refined structure of pseudomonas putida lipoamide dehydrogenase complexed with NAD+ at 2.45 angstroms resolution (see paper)
57% identity, 100% coverage: 2:464/464 of query aligns to 2:458/458 of 1lvlA

query
sites
1lvlA

K

Q

E

T

I

I

S

Q

C

T

K

T

L

L

V

I

L
x
I

G
|
G

A
x
G

G
|
G

P
|
P

G
|
G

G

G

Y

Y

V

V

A

A

I

I

R

R

A

A

G

G

Q

Q

L

L

G

G

V

I

N

P

T

T

V

V

I

L

V
|
V

E
|
E

K
x
G

A
x
Q

K

A

A
x
L

G

G

G
|
G

T
|
T

C
|
C

L

L

N

N

V
x
I

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

I

I

H

H

A

V

A

A

D

E

E

Q

Y

F

H

H

R

Q

L

-

R

A

A

S

A

R

A

F

S

T

G

E

K

P

G

S

P

P

L

L

G

G

L

I

S

S

L

V

S

A

A
x
S

P
|
P

A

R

I

L

D

D

L

I

R

G

R

Q

T

S

I

V

A

A

W

W

K

K

D

D

G

G

I

I

V

V

G

D

R

R

L

L

N

T

G

T

G

G

V

V

T

A

G

A

L

L

K

K

A

H

G

G

V

V

K

K

A

V

V

V

I

H

G
|
G

E
x
W

G
x
A

R

K

F

V

V

L

D

D

G

G

K

K

T

Q

V

V

D

E

V

V

E

D

T

G

E

-

T

-

G

-

L

-

Q

Q

R

R

I

I

R

Q

A

C

E

E

A

H

I

L

V

L

I

L

A
|
A

T
|
T

G
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G

S

S

A

S

P

S

V

V

E

E

L

L

P

P

D

M

L

L

P

P

F

L

G

G

S

P

V

V

I

I

S

S

S
|
S

T

T

Q

E

A

A

L

L

A

A

L

P

T

K

D

A

V

L

P

P

Q

Q

T

H

L

L

A

V

V

V

I
x
V

G
|
G

Y
|
Y

I
|
I

G

G

L

L

E
|
E

L

L

G

G

T

I

A

A

F

Y

A

R

K

K

L

L

G

G

S

A

K

Q

V

V

T

S

V

V

L
x
V

E
|
E

A
|
A

L
x
R

D

E

R

R

I

I

L

L

P
|
P

Q

T

Y

Y

D

D

A

S

D

E

L

L

S

T

K

A

P

P

V

V

M

A

K

E

R

S

L

L

G

K

E

K

L

L

G

G

V

I

E

A

V

L

F

H

T

L

R

-

T

-

A

-

A

G

K

H

R
x
S

L
x
V

S

E

A

G

D

Y

R

E

R

N

G

G

L

C

L

L

A

L

E

A

E

N

N

D

G

G

R

K

A

G

-

G

-

Q

F

L

E

R

V

L

P

E

A

A

E

D

K

R

V

V

L

L

V

V

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x
A

V
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V

G
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G

R
|
R

R

R

P

P

V
x
R

T

T

D

K

G

G

W
x
F

G

N

L

L

E

E

E

C

I

L

D

D

L

L

D

K

H

M

S

N

G

G

R

A

F

A

I

I

R

A

I

I

D

D

D

E

Q

R

C

C

R

Q

T

T

S

S

M

M

R

H

G

N

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Y

W

A

A

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I

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G

D
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D

V

V

T

A

G

G

E
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E

P
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P

M
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M

L
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L

A
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A

H
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H

R

R

A
|
A

M
|
M

A

A

Q

Q

G

G

E

E

M

M

V

V

A

A

E

E

I

I

V

I

A

A

G

G

H

K

K

A

R

R

S

R

W

F

D

E

K

P

R

A

C

A

I

I

P

A

A

A

V

V

C

C

F

F

T

T

D

D

P

P

E

E

I

V

V

V

G

V

A

V

G

G

L

K

S

T

P

P

E

E

E

Q

A

A

R

S

A

Q

G

G

I

L

D

D

V

C

K

I

I

V

G

A

Q

Q

F

F

P

P

F

F

Q

A

A

A

N

N

G

G

R

R

A

A

M

M

T

S

T

L

L

E

S

S

E

K

D

S

G

G

F

F

V

V

R

R

V

V

I

V

A

A

R

R

A

R

D

D

N

N

H

H

L

L

V

I

L

L

G

G

I

W

Q

Q

A

A

V

V

G

G

H

V

G

A

V

V

S

S

E

E

L

L

S

S

A

T

T

A

F

F

A

A

L

Q

A

S

I

L

E

E

M

M

G

G

A

A

R

C

L

L

E

E

D

D

I

V

A

A

G

G

T

T

I

I

H
|
H

A

A

H
|
H

P

P

T

T

Q

L

S

G

E
|
E

A

A

F

V

Q

Q

E

E

A

A

L

L

K

R

T

A

L

L

G

G

H

H

A

A

L

L

H

H

I

I

active site: P15 (= P15), L39 (≠ A39), C43 (= C43), C48 (= C48), S51 (= S51), S79 (≠ A80), P80 (= P81), Y181 (= Y186), E185 (= E190), M317 (= M323), H435 (= H441), H437 (= H443), E442 (= E448)
binding flavin-adenine dinucleotide: I11 (≠ L11), G12 (= G12), G13 (≠ A13), G14 (= G14), P15 (= P15), G16 (= G16), V34 (= V34), E35 (= E35), G36 (≠ K36), Q37 (≠ A37), G41 (= G41), T42 (= T42), C43 (= C43), I46 (≠ V46), G47 (= G47), C48 (= C48), K52 (= K52), G116 (= G117), W117 (≠ E118), A118 (≠ G119), A141 (= A146), T142 (= T147), G143 (= G148), S161 (= S166), Y181 (= Y186), I182 (= I187), R266 (= R272), R269 (≠ V275), F273 (≠ W279), G304 (= G310), D305 (= D311), M311 (= M317), L312 (= L318), A313 (= A319), H314 (= H320), A316 (= A322)
binding nicotinamide-adenine-dinucleotide: V177 (≠ I182), G178 (= G183), G179 (= G184), G180 (= G185), Y181 (= Y186), I182 (= I187), V200 (≠ L205), E201 (= E206), A202 (= A207), R203 (≠ L208), P208 (= P213), S234 (≠ R242), V235 (≠ L243), A263 (≠ T269), V264 (= V270), G265 (= G271), R266 (= R272), E309 (= E315), P310 (= P316), M311 (= M317)

P11959 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; EC 1.8.1.4 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
46% identity, 99% coverage: 4:464/464 of query aligns to 8:467/470 of P11959

query
sites
P11959

I

I

S

E

C

T

K

E

L

T

L

L

V

V

L

V

G

G

A

A

G

G

P

P

G

G

Y

Y

V

V

A

A

I

I

R

R

A

A

G

A

Q

Q

L

L

G

G

V

Q

N

K

T

V

V

T

I

I

V

V

E
|
E

K
|
K

A
x
G

K
x
N

A
x
L

G
|
G

T
x
V

C
|
C

L

L

N

N

V

V

G

G

C

C

I

I

P

P

S

S

K
|
K

A

A

L

L

I

I

H

S

A

A

A

S

D

H

E

R

Y

Y

H

E

R

Q

L

A

R

K

A

H

A

S

A

E

S

E

G

-

K

-

G

-

P

-

L

M

G

G

L

I

S

K

L

A

S

E

A

N

P

V

A

T

I

I

D

D

L

F

R

A

R

K

T

V

I

Q

A

E

W

W

K

K

D

A

G

S

I

V

V

V

G

K

R

K

L

L

N

T

G

G

V

V

T

E

G

G

L

L

K

K

K

G

A

N

G

K

V

V

K

E

A

I

V

V

I

K

G

G

E

E

G

A

R

Y

F

F

V

V

D

D

G

A

K

N

T

T

V

V

D

R

V

V

E

V

T

N

E

G

T

D

G

S

L

A

Q

Q

R

T

I

Y

R

T

A

F

E

K

A

N

I

A

V

I

I

I

A

A

T

T

G

G

S

S

A

R

P

P

V

I

E

E

L

L

P

P

D

N

L

F

P

K

F

F

G

S

G

N

S

R

V

I

I

L

S

D

S

S

T

T

Q

G

A

A

L

L

A

N

L

L

T

G

D

E

V

V

P

P

Q

K

T

S

L

L

A

V

V

V

I

I

G

G

Y

Y

I

I

G

G

L

I

E

E

L

L

G

G

T

T

A

A

F

Y

A

A

K

N

L

F

G

G

S

T

K

K

V

V

T

T

V

I

L

L

E

E

A

G

L

A

D

G

R

E

I

I

L

L

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S

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G

Y

F

D

E

A

K

D

Q

L

M

S

A

K

A

P

I

V

I

M

K

K

K

R

R

L

L

G

K

E

K

L

K

G

G

V

V

E

E

V

V

F

V

T

T

R

N

T

A

A

L

A

A

K

K

R

G

L

A

S

E

A

E

D

R

R

E

R

D

G

G

L

V

L

T

A

V

-

T

-

Y

E

E

E

A

N

N

G

G

R

E

A

T

F

K

E

T

V

I

P

D

A

A

E

D

K

Y

V

V

L

L

V

V

T

T

V

V

G

G

R

R

P

P

V

N

T

T

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D

G

E

W

L

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G

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L

E

E

E

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I

I

D

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L

I

D

K

H

M

S

T

G

N

R

R

-

G

F

L

I

I

R

E

I

V

D

D

D

Q

Q

Q

C

C

R

R

T

T

S

S

M

V

R

P

G

N

V

I

Y

F

A

A

I

I

G

G

D
|
D

V

I

T

V

G

P

E

G

P

P

M

A

L

L

A
|
A

H

H

R

K

A

A

M

S

A

Y

Q

E

G

G

E

K

M

V

V

A

A

A

E

E

I

A

V

I

A

A

G

G

H

H

K

P

R

S

S

A

W

V

D

D

K

Y

R

V

C

A

I

I

P

P

A

A

V

V

C

V

F

F

T

S

D

D

P

P

E

E

I

C

V

A

G

S

A

V

G

G

L

Y

S

F

P

E

E

Q

A

A

R

K

A

D

A

E

G

G

I

I

D

D

V

V

K

I

I

A

G

A

Q

K

F

F

P

P

F

F

Q

A

A

A

N

N

G

G

R

R

A

A

M

L

T

A

T

L

L

N

S

D

E

T

D

D

G

G

F

F

V

L

R

K

V

L

I

V

A

V

R

R

A

K

D

E

N

D

H

G

L

V

V

I

L

I

G

G

I

A

Q

Q

A

I

V

I

G

G

H

P

G

N

V

A

S

S

E

D

L

M

S

I

A

A

T

E

F

L

A

G

L

L

A

A

I

I

E

E

M

A

G

G

A

M

R

T

L

A

E

E

D

D

I

I

A

A

G

L

T

T

I

I

H

H

A

A

H

H

P

P

T

T

Q

L

S

G

E

E

A

I

F

A

Q

M

E

E

A

A

L

E

K

V

T

A

L

L

G

G

H

T

A

P

L

I

H

H

I

I

39:47 (vs. 35:43, 56% identical) binding
K56 (= K52) binding
D314 (= D311) binding
A322 (= A319) binding

1ebdA Dihydrolipoamide dehydrogenase complexed with the binding domain of the dihydrolipoamide acetylase (see paper)
46% identity, 97% coverage: 4:454/464 of query aligns to 2:451/455 of 1ebdA

query
sites
1ebdA

I

I

S

E

C

T

K

E

L

T

L

L

V

V

L
x
V

G
|
G

A

A

G
|
G

P
|
P

G
|
G

G

G

Y

Y

V

V

A

A

I

I

R

R

A

A

G

A

Q

Q

L

L

G

G

V

Q

N

K

T

V

V

T

I

I

V
|
V

E
|
E

K
|
K

A

G

K

N

A
x
L

G

G

G
|
G

T
x
V

C
|
C

L

L

N

N

V
|
V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

I

I

H

S

A

A

A

S

D

H

E

R

Y

Y

H

E

R

Q

L

A

R

K

A

H

A

S

A

E

S

E

G

-

K

-

G

-

P

-

L

M

G

G

L

I

S

K

L

A

S

E

A
x
N

P
x
V

A

T

I

I

D

D

L

F

R

A

R

K

T

V

I

Q

A

E

W

W

K

K

D

A

G

S

I

V

V

V

G

K

R

K

L

L

N

T

G

G

V

V

T

E

G

G

L

L

K

K

K

G

A

N

G

K

V

V

K

E

A

I

V

V

I

K

G
|
G

E
|
E

G
x
A

R

Y

F

F

V

V

D

D

G

A

K

N

T

T

V

V

D

R

V

V

E

V

T

N

E

G

T

D

G

S

L

A

Q

Q

R

T

I

Y

R

T

A

F

E

K

A

N

I

A

V

I

I

I

A
|
A

T
|
T

G
|
G

S

S

A

R

P

P

V

I

E

E

L

L

P

P

D

N

L

F

P

K

F

F

G

S

G

N

S

R

V

I

I

L

S

D

S
|
S

T

T

Q

G

A

A

L

L

A

N

L

L

T

G

D

E

V

V

P

P

Q

K

T

S

L

L

A

V

V

V

I

I

G

G

Y
|
Y

I
|
I

G

G

L

I

E
|
E

L

L

G

G

T

T

A

A

F

Y

A

A

K

N

L

F

G

G

S

T

K

K

V

V

T

T

V

I

L

L

E

E

A

G

L

A

D

G

R

E

I

I

L

L

P

S

Q

G

Y

F

D

E

A

K

D

Q

L

M

S

A

K

A

P

I

V

I

M

K

K

K

R

R

L

L

G

K

E

K

L

K

G

G

V

V

E

E

V

V

F

V

T

T

R

N

T

A

A

L

A

A

K

K

R

G

L

A

S

E

A

E

D

R

R

E

R

D

G

G

L

V

L

T

A

V

-

T

-

Y

E

E

E

A

N

N

G

G

R

E

A

T

F

K

E

T

V

I

P

D

A

A

E

D

K

Y

V

V

L

L

V

V

T

T

V

V

G

G

R
|
R

R

R

P

P

V

N

T

T

D

D

G

E

W

L

G

G

L

L

E

E

E

Q

I

I

D

G

L

I

D

K

H

M

S

T

G

N

R

R

-

G

F

L

I

I

R

E

I

V

D

D

D

Q

Q

Q

C

C

R

R

T

T

S

S

M

V

R

P

G

N

V

I

Y

F

A

A

I

I

G
|
G

D
|
D

V

I

T

V

G

P

E

G

P

P

M
x
A

L
|
L

A
|
A

H

H

R

K

A
|
A

M
x
S

A

Y

Q

E

G

G

E

K

M

V

V

A

A

A

E

E

I

A

V

I

A

A

G

G

H

H

K

P

R

S

S

A

W

V

D

D

K

Y

R

V

C

A

I

I

P

P

A

A

V

V

C

V

F

F

T

S

D

D

P

P

E

E

I

C

V

A

G

S

A

V

G

G

L

Y

S

F

P

E

E

Q

A

A

R

K

A

D

A

E

G

G

I

I

D

D

V

V

K

I

I

A

G

A

Q

K

F

F

P

P

F

F

Q

A

A

A

N

N

G

G

R

R

A

A

M

L

T

A

T

L

L

N

S

D

E

T

D

D

G

G

F

F

V

L

R

K

V

L

I

V

A

V

R

R

A

K

D

E

N

D

H

G

L

V

V

I

L

I

G

G

I

A

Q

Q

A

I

V

I

G

G

H

P

G

N

V

A

S

S

E

D

L

M

S

I

A

A

T

E

F

L

A

G

L

L

A

A

I

I

E

E

M

A

G

G

A

M

R

T

L

A

E

E

D

D

I

I

A

A

G

L

T

T

I

I

H
|
H

A

A

H
|
H

P
|
P

T

T

Q

L

S

G

E
|
E

A

I

F

A

Q

M

E

E

A

A

active site: P13 (= P15), L37 (≠ A39), C41 (= C43), C46 (= C48), S49 (= S51), N74 (≠ A80), V75 (≠ P81), Y180 (= Y186), E184 (= E190), S320 (≠ M323), H438 (= H441), H440 (= H443), E445 (= E448)
binding flavin-adenine dinucleotide: V9 (≠ L11), G10 (= G12), G12 (= G14), P13 (= P15), G14 (= G16), V32 (= V34), E33 (= E35), K34 (= K36), G39 (= G41), V40 (≠ T42), C41 (= C43), V44 (= V46), G45 (= G47), C46 (= C48), K50 (= K52), G111 (= G117), E112 (= E118), A113 (≠ G119), A140 (= A146), T141 (= T147), G142 (= G148), S160 (= S166), Y180 (= Y186), I181 (= I187), R268 (= R272), G307 (= G310), D308 (= D311), A314 (≠ M317), L315 (= L318), A316 (= A319), A319 (= A322), H440 (= H443), P441 (= P444)

P18925 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; EC 1.8.1.4 from Azotobacter vinelandii (see 2 papers)
44% identity, 98% coverage: 8:464/464 of query aligns to 7:472/477 of P18925

query
sites
P18925

L

V

L

I

V

V

L

I

G

G

A

A

G

G

P

P

G

G

Y

Y

V

V

A

A

I

I

R

K

A

S

G

A

Q

Q

L

L

G

G

V

L

N

K

T

T

V

A

I

L

V

I

E
|
E

K
|
K

A
x
Y

K
|
K

A
x
G

-
x
K

-
x
E

-
x
G

-
x
K

-
x
T

-
x
A

-
x
L

G
|
G

T
|
T

C
|
C

L

L

N

N

V

V

G

G

C
|
C

I

I

P

P

S

S

K
|
K

A

A

L

L

I

L

H

D

A

S

D

Y

E

K

Y

F

H

H

R

E

L

A

R

H

A

E

A

S

A

F

S

K

G

L

K

H

G

G

P

-

L

-

G

-

L

I

S

S

L

T

S

G

A

E

P

V

A

A

I

I

D

D

L

V

R

P

R

T

T

M

I

I

A

A

W

R

K

K

D

D

G

Q

I

I

V

V

G

R

R

N

L

L

N

T

G

G

V

V

T

A

G

S

L

L

L

I

K

K

K

A

A

N

G

G

V

V

K

T

A

L

V

F

I

E

G

G

E

H

G

G

R

K

F

L

V

L

D

A

G

G

K

K

T

K

V

V

D

E

V

V

E

T

T

A

E

A

T

D

G

G

L

S

-

S

Q

Q

R

V

I

L

R

D

A

T

E

E

A

N

I

V

V

I

I

L

A

A

T

S

G

G

S

S

A

K

P

P

V

V

E

E

L

I

P

P

D

P

L

A

P

P

F

V

G

D

G

Q

S

D

V

V

I

I

-

V

S

D

S

S

T

T

Q

G

A

A

L

L

A

D

L

F

T

Q

D

N

V

V

P

P

Q

G

T

K

L

L

A

G

V

V

I

I

G

G

G

A

G

G

Y

V

I

I

G

G

L

L

E

E

L

L

G

G

T

S

A

V

F

W

A

A

K

R

L

L

G

G

S

A

K

E

V

V

T

T

V

V

L

L

E

E

A

A

L

M

D

D

R

K

I

F

L

L

P

P

Q

A

Y

V

D

D

A

E

D

Q

L

V

S

A

K

K

P

E

V

A

M

Q

K

K

R

I

L

L

G

T

E

K

L

Q

G

G

V

L

E

K

V

I

F

L

-

L

-

G

-

A

-

R

-

V

T

T

R

G

T

T

A

E

A

V

K

K

R

N

L

K

S

Q

A

V

D

T

R

V

R

K

G

F

L

V

L

D

A

A

E

E

-

G

E

E

N

K

G

S

R

Q

A

A

F

F

E

D

V

-

P

-

A

-

E

-

K

K

V

L

L

I

V

V

T

A

V

V

G

G

R

R

P

P

V

V

T

T

D

T

G

D

W

L

G

L

L

A

E

A

E

D

-

S

-

G

I

V

D

T

L

L

D

D

H

E

S

R

G

G

R

-

F

F

I

I

R

Y

I

V

D

D

Q

Y

C

C

R

A

T

T

S

S

M

V

R

P

G

G

V

V

Y

Y

A

A

I

I

G

G

D
|
D

V

V

T

V

G

R

E

G

P

A

M

M

L

L

A
|
A

H

H

R

K

A

A

M

S

A

E

Q

E

G

G

E

V

M

V

V

V

A

A

E

E

I

R

V

I

A

A

G

G

H

H

K

K

R

A

S

Q

W

M

D

N

K

Y

R

D

C

L

I

I

P

P

A

A

V

V

C

I

F

Y

T

T

D

H

P

P

E

E

I

I

V

A

G

G

A

V

G

G

L

K

S

T

P

E

E

Q

E

A

A

L

R

K

A

A

A

E

G

G

I

V

D

A

V

I

K

N

I

V

G

G

Q

V

F

F

P

P

F

F

Q

A

A

A

N

S

G

G

R

R

A

A

M

M

T

A

L

N

S

D

E

T

D

A

G

G

F

F

V

V

R

K

V

V

I

I

A

A

R

D

A

A

D

K

N

T

H

D

L

R

V

V

L

L

G

G

I

V

Q

H

A

V

V

I

G

G

H

P

G

S

V

A

S

A

E

E

L

L

S

V

A

Q

T

Q

F

G

A

A

L

I

A

A

I

M

E

E

M

F

G

G

A

T

R

S

L

A

E

E

D

D

I

L

A

G

G

M

T

M

I

V

H

F

A

A

H

H

P

P

T

A

Q

L

S

S

E

E

A

A

F

L

Q

H

E

E

A

A

L

L

K

A

T

V

L

S

G

G

H

H

A

A

L

I

H

H

I

V

34:49 (vs. 35:43, 44% identical) binding
C49 (= C43) modified: Disulfide link with 54, Redox-active
C54 (= C48) modified: Disulfide link with 49, Redox-active
K58 (= K52) binding
D319 (= D311) binding
A327 (= A319) binding

3ladA Refined crystal structure of lipoamide dehydrogenase from azotobacter vinelandii at 2.2 angstroms resolution. A comparison with the structure of glutathione reductase (see paper)
44% identity, 98% coverage: 8:464/464 of query aligns to 6:471/472 of 3ladA

query
sites
3ladA

L

V

L

I

V

V

L
x
I

G
|
G

A
|
A

G
|
G

P
|
P

G
|
G

G

G

Y

Y

V

V

A

A

I

I

R

K

A

S

G

A

Q

Q

L

L

G

G

V

L

N

K

T

T

V

A

I

L

V
x
I

E
|
E

K
|
K

A
x
Y

K

K

A

G

-

K

-

E

-

G

-

K

-

T

-

A

-
x
L

G

G

G
|
G

T
|
T

C
|
C

L

L

N

N

V
|
V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

I

L

H

D

A

S

D

Y

E

K

Y

F

H

H

R

E

L

A

R

H

A

E

A

S

A

F

S

K

G

L

K

H

G

G

P

-

L

-

G

-

L

I

S

S

L

T

S

G

A

E

P

V

A

A

I

I

D

D

L

V

R

P

R

T

T

M

I

I

A

A

W

R

K

K

D

D

G

Q

I

I

V

V

G

R

R

N

L

L

N

T

G

G

V

V

T

A

G

S

L

L

L

I

K

K

K

A

A

N

G

G

V

V

K

T

A

L

V

F

I

E

G
|
G

E
x
H

G
|
G

R

K

F

L

V

L

D

A

G

G

K

K

T

K

V

V

D

E

V

V

E

T

T

A

E

A

T

D

G

G

L

S

-

S

Q

Q

R

V

I

L

R

D

A

T

E

E

A

N

I

V

V

I

I

L

A
|
A

T
x
S

G
|
G

S
|
S

A

K

P

P

V

V

E

E

L

I

P

P

D

P

L

A

P

P

F

V

G

D

G

Q

S

D

V

V

I

I

-

V

S

D

S
|
S

T

T

Q

G

A

A

L

L

A

D

L

F

T

Q

D

N

V

V

P

P

Q

G

T

K

L

L

A

G

V

V

I

I

G

G

G

A

G

G

Y
x
V

I
|
I

G

G

L

L

E
|
E

L

L

G

G

T

S

A

V

F

W

A

A

K

R

L

L

G

G

S

A

K

E

V

V

T

T

V

V

L

L

E

E

A

A

L

M

D

D

R

K

I

F

L

L

P

P

Q

A

Y

V

D

D

A

E

D

Q

L

V

S

A

K

K

P

E

V

A

M

Q

K

K

R

I

L

L

G

T

E

K

L

Q

G

G

V

L

E

K

V

I

F

L

-

L

-

G

-

A

-

R

-

V

T

T

R

G

T

T

A

E

A

V

K

K

R

N

L

K

S

Q

A

V

D

T

R

V

R

K

G

F

L

V

L

D

A

A

E

E

-

G

E

E

N

K

G

S

R

Q

A

A

F

F

E

D

V

-

P

-

A

-

E

-

K

K

V

L

L

I

V

V

T

A

V

V

G

G

R
|
R

R

R

P

P

V

V

T

T

D

T

G

D

W

L

G

L

L

A

E

A

E

D

-

S

-

G

I

V

D

T

L

L

D

D

H

E

S

R

G

G

R

-

F

F

I

I

R

Y

I

V

D

D

Q

Y

C

C

R

A

T

T

S

S

M

V

R

P

G

G

V

V

Y

Y

A

A

I

I

G
|
G

D
|
D

V

V

T

V

G

R

E

G

P

A

M
|
M

L
|
L

A
|
A

H
|
H

R

K

A
|
A

M

S

A

E

Q

E

G

G

E

V

M

V

V

V

A

A

E

E

I

R

V

I

A

A

G

G

H

H

K

K

R

A

S

Q

W

M

D

N

K

Y

R

D

C

L

I

I

P

P

A

A

V

V

C

I

F
x
Y

T

T

D

H

P

P

E

E

I

I

V

A

G

G

A

V

G

G

L

K

S

T

P

E

E

Q

E

A

A

L

R

K

A

A

A

E

G

G

I

V

D

A

V

I

K

N

I

V

G

G

Q

V

F

F

P

P

F

F

Q

A

A

A

N

S

G

G

R

R

A

A

M

M

T

A

L

N

S

D

E

T

D

A

G

G

F

F

V

V

R

K

V

V

I

I

A

A

R

D

A

A

D

K

N

T

H

D

L

R

V

V

L

L

G

G

I

V

Q

H

A

V

V

I

G

G

H

P

G

S

V

A

S

A

E

E

L

L

S

V

A

Q

T

Q

F

G

A

A

L

I

A

A

I

M

E

E

M

F

G

G

A

T

R

S

L

A

E

E

D

D

I

L

A

G

G

M

T

M

I

V

H
x
F

A

A

H
|
H

P
|
P

T

A

Q

L

S

S

E
|
E

A

A

F

L

Q

H

E

E

A

A

L

L

K

A

T

V

L

S

G

G

H

H

A

A

L

I

H

H

I

V

active site: L44 (vs. gap), C48 (= C43), C53 (= C48), S56 (= S51), V190 (≠ Y186), E194 (= E190), F448 (≠ H441), H450 (= H443), E455 (= E448)
binding flavin-adenine dinucleotide: I9 (≠ L11), G10 (= G12), A11 (= A13), G12 (= G14), P13 (= P15), G14 (= G16), I32 (≠ V34), E33 (= E35), K34 (= K36), Y35 (≠ A37), G46 (= G41), T47 (= T42), C48 (= C43), V51 (= V46), G52 (= G47), C53 (= C48), S56 (= S51), K57 (= K52), G119 (= G117), H120 (≠ E118), G121 (= G119), A149 (= A146), S150 (≠ T147), G151 (= G148), S152 (= S149), S170 (= S166), I191 (= I187), R278 (= R272), G317 (= G310), D318 (= D311), M324 (= M317), L325 (= L318), A326 (= A319), H327 (= H320), A329 (= A322), Y357 (≠ F350), H450 (= H443), P451 (= P444)

3urhB Crystal structure of a dihydrolipoamide dehydrogenase from sinorhizobium meliloti 1021
43% identity, 98% coverage: 8:463/464 of query aligns to 3:465/465 of 3urhB

query
sites
3urhB

L

L

L

I

V

V

L
x
I

G
|
G

A
x
S

G
|
G

P
|
P

G
|
G

G

G

Y

Y

V

V

A

C

A

A

I

I

R

K

A

A

G

A

Q

Q

L

L

G

G

V

M

N

K

T

V

V

A

I

V

V
|
V

E
|
E

K
|
K

-

R

A

S

K

T

A
x
Y

G

G

G
|
G

T
|
T

C
|
C

L

L

N

N

V
|
V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

A

A

L

L

I

L

H

H

A

A

A

S

D

E

E

M

Y

F

H

H

R

Q

L

-

R

-

A

-

A

A

A

Q

S

H

G

G

K

L

G

E

P

A

L

L

G

G

L

V

S

E

L

V

S

A

A

N

P

P

A

K

I

L

D

N

L

L

R

Q

R

K

T

M

I

M

A

A

W

H

K

K

D

D

G

A

I

T

V

V

G

K

R

S

L

N

N

V

G

D

G

G

V

V

T

S

G

F

L

L

L

F

K

K

A

N

G

K

V

I

K

D

A

G

V

F

I

Q

G
|
G

E
x
T

G
|
G

R

K

F

V

V

L

D

G

G

Q

K

G

T

K

V

V

D

S

V

V

E

T

T

N

E

E

T

K

G

G

L

E

Q

E

R

Q

I

V

-

L

R

E

A

A

E

K

A

N

I

V

V

V

I

I

A
|
A

T
|
T

G
|
G

S

S

A

D

P

V

V

A

E

G

L

I

P

P

D

G

L

V

P

E

F

V

G

A

-

F

-

D

-

E

G

K

S

T

V

I

I

V

S

S

S
|
S

T

T

Q

G

A

A

L

L

A

A

L

L

T

E

D

K

V

V

P

P

Q

A

T

S

L

M

A

I

V

V

I

V

G

G

Y
x
V

I
|
I

G

G

L

L

E
|
E

L

L

G

G

T

S

A

V

F

W

A

A

K

R

L

L

G

G

S

A

K

K

V

V

T

T

V

V

L

V

E

E

A

F

L

L

D

D

R

T

I

I

L

L

P

G

Q

G

Y

M

D

D

A

G

D

E

L

V

S

A

K

K

P

Q

V

L

M

Q

K

R

R

M

L

L

G

T

E

K

L

Q

G

G

V

I

E

D

V

F

F

-

T

-

R

K

T

L

A

G

A

A

K

K

R

V

L

T

S

G

A

A

D

V

R

K

R

S

G

G

L

D

L

G

A

A

E

K

-

V

-

T

-

F

-

E

-

P

-

V

E

K

N

G

G

G

R

E

A

A

F

T

E

T

V

L

P

D

A

A

E

E

K

V

V

V

L

L

V

I

T

A

V

T

G

G

R
|
R

R

K

P

P

V

S

T

T

D

D

G
|
G

W

L

G

G

L

L

E

A

E

K

-

A

-

G

I

V

D

V

L

L

D

D

H

S

S

R

G

G

R

R

F

-

I

V

R

E

I

I

D

D

D

R

Q

H

C

F

R

Q

T

T

S

S

M

I

R

A

G

G

V

V

Y

Y

A

A

I

I

G
|
G

D
|
D

V

V

T

V

G

R

E

G

P

P

M
|
M