SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SMc03840 FitnessBrowser__Smeli:SMc03840 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 14 hits to proteins with known functional sites (download)

4jxrB Crystal structure of a gnat superfamily phosphinothricin acetyltransferase (pat) from sinorhizobium meliloti in complex with accoa
100% identity, 100% coverage: 1:185/185 of query aligns to 1:185/185 of 4jxrB

query
sites
4jxrB

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binding acetyl coenzyme *a: A29 (= A29), D86 (= D86), S87 (= S87), I88 (= I88), Y89 (= Y89), L90 (= L90), P92 (= P92), R95 (= R95), G96 (= G96), G100 (= G100), H127 (= H127), S129 (= S129), S130 (= S130), H134 (= H134)

5dwnA Crystal structure of phosphinothricin n-acetyltransferase from brucella ovis in complex with acetylcoa
53% identity, 92% coverage: 4:173/185 of query aligns to 5:175/181 of 5dwnA

query
sites
5dwnA

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binding acetyl coenzyme *a: S87 (= S87), I88 (= I88), Y89 (= Y89), I90 (≠ L90), K95 (≠ R95), G96 (= G96), G98 (= G98), G100 (= G100), K101 (= K101), G131 (≠ S129), S132 (= S130), K134 (≠ A132), L135 (= L133), H136 (= H134)

A6VCX3 L-methionine sulfoximine/L-methionine sulfone acetyltransferase; Methionine derivative detoxifier A; MDDA; EC 2.3.1.- from Pseudomonas aeruginosa (strain PA7) (see paper)
44% identity, 89% coverage: 1:165/185 of query aligns to 1:165/172 of A6VCX3

query
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A6VCX3

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RPF 75:77 (≠ RNR 75:77) binding
EHS 85:87 (≠ EDS 85:87) binding

2j8mA Structure of p. Aeruginosa acetyltransferase pa4866 (see paper)
43% identity, 89% coverage: 2:165/185 of query aligns to 1:164/171 of 2j8mA

query
sites
2j8mA

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binding azide ion: W73 (≠ F74), R74 (= R75)

2j8rA Structure of p. Aeruginosa acetyltransferase pa4866 solved in complex with l-methionine sulfoximine (see paper)
44% identity, 88% coverage: 3:165/185 of query aligns to 1:163/170 of 2j8rA

query
sites
2j8rA

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binding azide ion: W42 (≠ R44), Y66 (= Y68), S85 (= S87)
binding (2s)-2-amino-4-(methylsulfonimidoyl)butanoic acid: A27 (= A29), I28 (≠ T30), W29 (≠ Y31), R73 (= R75), F75 (≠ R77), F78 (≠ Y80), E83 (= E85)

2jlmF Structure of a putative acetyltransferase (aciad1637) from acinetobacter baylyi adp1 (see paper)
38% identity, 81% coverage: 16:165/185 of query aligns to 22:171/180 of 2jlmF

query
sites
2jlmF

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binding azide ion: T34 (≠ V28), A35 (= A29), Y95 (= Y89), I96 (≠ L90), D130 (≠ G124), N133 (≠ H127)

3dr8A Structure of ynca, a putative acetyltransferase from salmonella typhimurium with its cofactor acetyl-coa
38% identity, 90% coverage: 4:170/185 of query aligns to 4:169/173 of 3dr8A

query
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3dr8A

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A

A

binding acetyl coenzyme *a: A29 (= A29), H85 (≠ D86), S86 (= S87), V87 (≠ I88), Y88 (= Y89), V89 (≠ L90), Q94 (≠ R95), G95 (= G96), G97 (= G98), G99 (= G100), R100 (≠ K101), A127 (≠ P128), A128 (≠ S129), R131 (≠ A132), H133 (= H134), S135 (≠ A136)

Q8ZPD3 L-methionine sulfoximine/L-methionine sulfone acetyltransferase; L-amino acid N-acyltransferase; Methionine derivative detoxifier A; MDDA; EC 2.3.1.-; EC 2.3.1.- from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
38% identity, 90% coverage: 4:170/185 of query aligns to 2:167/171 of Q8ZPD3

query
sites
Q8ZPD3

T

T

L

I

R

R

D

F

A

A

V

D

A

K

A

A

D

D

L

C

R

A

S

A

I

I

T

T

E

E

I

I

Y

Y

R

N

E

H

S

A

V

V

L

L

N

H

G

T

V

A

A

A

T

I

Y

W

E

N

E

D

T

R

P

T

P

V

S

D

E

T

A

D

E

N

M

R

A

L

L

A

R

W

F

Y

S

E

T

A

I

R

T

Q

G

L

N

L

G

G

Y

Y

P

P

Y

V

V

L

V

V

A

S

L

-

D

E

E

E

R

N

G

G

A

V

V

V

I

T

G

G

Y

Y

A

A

Y

S

A

F

S

G

A

D

F

W

R

R

N

S

R

F

T

D

A

G

Y

F

R

R

F

Y

L

T

V

V

E
|
E

D

H

S

S

I
x
V

Y
|
Y

L
x
V

S

H

P

P

E

A

A

H

R

Q

G
|
G

K
|
K

G
|
G

I
x
L

G
|
G

K
x
R

A

K

L

L

S

S

E

R

L

L

V

I

G

D

R

E

C

A

T

R

A

R

L

C

G

G

F

K

R

H

Q

V

M

M

I

V

A

A

V

G

I

I

G

E

G

S

A

Q

H
x
N

P

A

S

A

S

S

I

I

A

R

L

L

H

H

R

H

A
x
S

L

L

G

G

F

F

E

T

L

V

Q

T

G

A

L

Q

M

M

K

P

A

Q

T

V

G

G

F

V

K

K

H

F

G

G

R

R

W

W

L

L

D

D

T

L

A

T

F

F

M

M

Q

Q

R

L

P

Q

L

L

G

D

E

E

G

H

T

A

A

A

E82 (= E85) mutation to Q: Loss of acetyltransferase activity.
VYV 85:87 (≠ IYL 88:90) binding
GKGLGR 93:98 (≠ GKGIGK 96:101) binding
N124 (≠ H127) binding
S133 (≠ A136) binding

4mbuA Crystal structure of n-acetyltransferase from staphylococcus aureus mu50 (see paper)
35% identity, 89% coverage: 2:165/185 of query aligns to 2:164/165 of 4mbuA

query
sites
4mbuA

T

N

A

A

T

M

L

I

R

R

D

C

A

A

V

K

A

K

A

E

D

D

L

L

R

N

S

A

I

I

T

L

E

A

I

I

Y

Y

R

N

E

D

S

A

V

I

L

I

N

N

G

T

V

T

A

A

T

V

Y

Y

E

T

E

Y

T

K

P

P

P

Q

S

T

E

I

A

D

E

E

M

R

A

I

L

A

R

W

F

F

S

E

T

T

I

K

T

Q

G

R

N

N

G

H

Y

E

P

P

Y

-

V

I

V

F

A

V

L

F

D

E

E

E

R

N

G

G

A

S

V

V

I

L

G

G

Y

F

A

A

Y

T

A

F

S

G

A

S

F

F

R

R

N

P

R

W

T

P

A

A

Y

Y

R

Q

F

Y

L

T

V

I

E

E

D

H

S

S

I

I

Y

Y

L

V

S

D

P

A

E

S

A

A

R

R

G

G

K

K

G

G

I

I

G

A

K

S

A

Q

L

L

S

Q

E

R

L

L

V

I

G

V

R

E

C

A

T

K

A

A

L

K

G

G

F

Y

R

R

Q

T

M

L

I

V

A

A

V

G

I

I

G

D

G

A

A

S

H

N

P

E

S

A

S

S

I

I

A

K

L

L

H

H

R
x
Q

A

K

L

F

G

N

F

F

E
x
K

L
x
H

Q

A

G

G

L

T

M

L

K

T

A

N

T

V

G

G

F

Y

K

K

H

F

G

D

R

Y

W

W

L

L

D

D

T

L

A

A

F

F

M

Y

Q

E

R

L

P

D

L

L

binding phosphate ion: Q134 (≠ R135), K139 (≠ E140), H140 (≠ L141)

4jwpA Crystal structure of ribosomal-protein-alanine n-acetyltransferase from brucella melitensis in complex with acetyl coa
39% identity, 89% coverage: 1:165/185 of query aligns to 2:165/165 of 4jwpA

query
sites
4jwpA

M

M

T

T

A

L

T

L

L

I

R

R

D

H

A

A

V

T

A

E

A

A

D

D

L

L

R

P

S

A

I

L

T

L

E

A

I

I

Y

Y

R

N

E

D

S

A

V

V

L

E

N

N

G

T

V

L

A
|
A

T

I

Y

W

E

N

E

E

T

T

P

L

P

V

S

D

E

L

A

E

E

N

M

R

A

H

L

Q

R

W

F

L

S

E

T

N

I

R

T

N

G

R

N

D

G

G

Y

F

P

P

Y

V

V

L

V

V

A

A

L

-

D

E

E

R

R

E

G

G

A

Q

V

V

I

V

G

G

Y

Y

A

A

Y

S

A

Y

S

G

A

P

F

F

R

R

N

P

R

F

T

E

A

G

Y

F

R

R

F

H

L

S

V

S

E

E

D

L

S

S

I
x
V

Y
|
Y

L
x
V

S

A

P

S

E

N

A

A

R
|
R

G
|
G

K

G

G
|
G

I

I

G
|
G

K
x
R

A

T

L

L

S

A

E

E

L

L

V

I

G

E

R
x
E

C

A

T

R

A
x
E

L

R

G

K

F

V

R

H

Q

V

M

L

I

I

A

A

V
x
G

I

I

G

E

G

A

A

G

H

N

P

A

S
x
A

S
|
S

I

I

A
|
A

L

L

H

H

R

R

A

S

L

Q

G

G

F

F

E

E

L

E

Q

C

G

G

L

T

M

L

K

K

A

Q

T

V

G

G

F

Q

K

K

H

F

G

G

R

R

W

W

L

L

D

D

T

L

A

L

F

F

M

M

Q

Q

R

K

P

I

L

L

binding acetyl coenzyme *a: A30 (= A29), V88 (≠ I88), Y89 (= Y89), V90 (≠ L90), R95 (= R95), G96 (= G96), G98 (= G98), G100 (= G100), R101 (≠ K101), G122 (≠ V122), A129 (≠ S129), S130 (= S130), A132 (= A132)
binding calcium ion: E110 (≠ R110), E113 (≠ A113)

5t7eD Crystal structure of streptomyces hygroscopicus bialaphos resistance (bar) protein in complex with coenzyme a and l-phosphinothricin (see paper)
33% identity, 86% coverage: 3:162/185 of query aligns to 2:159/175 of 5t7eD

query
sites
5t7eD

A

A

T

D

L

I

R

R

D

R

A

A

V

T

A

E

A

A

D

D

L

M

R

P

S

A

I

V

T

C

E

T

I

I

Y

V

R

N

E

H

S
x
Y

V

I

L

E

N

T

G

S

V

T

A
x
V

T

N

Y
x
F

E

R

E

T

T

E

P

P

P

Q

S

E

E

P

A

Q

E

E

M

W

A

T

L

D

R

D

F

L

S

V

T

R

I

L

T

R

G

E

N

R

G

-

Y

Y

P

P

Y

W

V

L

V

V

A

A

L

-

D

E

E

V

R

D

G

G

A

E

V

V

I

A

G

G

Y

I

A

A

Y

Y

A

A

S

G

A

P

F

W

R
x
K

N

A

R
|
R

T

N

A

A

Y
|
Y

R

D

F

W

L

T

V

A

E

E

D

S

S
x
T

I
x
V

Y
|
Y

L
x
V

S

S

P

P

E

R

A

H

R
x
Q

G
x
R

K
x
T

G
|
G

I

L

G
|
G

K
x
S

A

T

L

L

L

Y

S

T

E

H

L

L

V

L

G

K

R

S

C

L

T

E

A

A

L

Q

G

G

F

F

R

K

Q

S

M

V

I

V

A

A

V
|
V

I

I

G
|
G

G

L

A

P

H

N

P

D

S
x
P

S
|
S

I

V

A

R

L
x
M

H

H

R

E

A

A

L

L

G

G

F

Y

E

A

L

P

Q

R

G

G

L

M

M

L

K

R

A

A

T

A

G

G

F

F

K

K

H

H

G

G

R

N

W

W

L

H

D

D

T

V

A

G

F

F

M

W

Q

Q

binding coenzyme a: Y22 (≠ S23), V28 (≠ A29), V85 (≠ I88), Y86 (= Y89), V87 (≠ L90), Q92 (≠ R95), R93 (≠ G96), T94 (≠ K97), G95 (= G98), G97 (= G100), S98 (≠ K101), P126 (≠ S129), S127 (= S130), M130 (≠ L133)
binding phosphinothricin: F30 (≠ Y31), K72 (≠ R75), R74 (= R77), Y77 (= Y80), T84 (≠ S87), Y86 (= Y89), V119 (= V122), G121 (= G124)

5t7dA Crystal structure of streptomyces hygroscopicus bialaphos resistance (bar) protein in complex with acetyl coenzyme a (see paper)
33% identity, 86% coverage: 3:162/185 of query aligns to 1:158/173 of 5t7dA

query
sites
5t7dA

A

A

T

D

L

I

R

R

D

R

A

A

V

T

A

E

A

A

D

D

L

M

R

P

S

A

I

V

T

C

E

T

I

I

Y

V

R

N

E

H

S
x
Y

V

I

L

E

N

T

G

S

V

T

A
x
V

T

N

Y

F

E

R

E

T

T

E

P

P

P

Q

S

E

E

P

A

Q

E

E

M

W

A

T

L

D

R

D

F

L

S

V

T

R

I

L

T

R

G

E

N

R

G

-

Y

Y

P

P

Y

W

V

L

V

V

A

A

L

-

D

E

E

V

R

D

G

G

A

E

V

V

I

A

G

G

Y

I

A

A

Y

Y

A

A

S

G

A

P

F

W

R

K

N

A

R

R

T

N

A

A

Y

Y

R

D

F

W

L

T

V

A

E

E

D

S

S
x
T

I
x
V

Y
|
Y

L
x
V

S

S

P

P

E

R

A

H

R
x
Q

G
x
R

K

T

G
|
G

I

L

G
|
G

K
x
S

A

T

L

L

L

Y

S

T

E

H

L

L

V

L

G

K

R

S

C

L

T

E

A

A

L

Q

G

G

F

F

R

K

Q

S

M

V

I

V

A

A

V
|
V

I

I

G

G

G

L

A

P

H

N

P

D

S
x
P

S
|
S

I

V

A
x
R

L
x
M

H

H

R

E

A

A

L

L

G

G

F

Y

E

A

L

P

Q

R

G

G

L

M

M

L

K

R

A

A

T

A

G

G

F

F

K

K

H

H

G

G

R

N

W

W

L

H

D

D

T

V

A

G

F

F

M

W

Q

Q

binding acetyl coenzyme *a: Y21 (≠ S23), V27 (≠ A29), T83 (≠ S87), V84 (≠ I88), Y85 (= Y89), V86 (≠ L90), Q91 (≠ R95), R92 (≠ G96), G94 (= G98), G96 (= G100), S97 (≠ K101), V118 (= V122), P125 (≠ S129), S126 (= S130), R128 (≠ A132), M129 (≠ L133)

5wphA Crystal structure of arsn, n-acetyltransferase with substrate ast from pseudomonas putida kt2440 (see paper)
37% identity, 95% coverage: 5:180/185 of query aligns to 4:170/179 of 5wphA

query
sites
5wphA

L

I

R

R

D

V

A

A

V

R

A

P

A

E

D

D

L

A

R

E

S

E

I

I

T

Q

E

I

I

I

Y

Y

R

A

E

P

S

I

V

V

L

L

N

N

G

T

V

A

A

I

T
x
S

Y
x
F

E

E

T

A

P

V

P

P

S

S

E

V

A

E

E

Q

M

M

A

R

L

E

R

R

F

I

S

S

T

T

I

-

T

T

G

L

N

Q

G

T

Y

Y

P

P

Y

Y

V

L

V

V

A

A

L

V

D

R

E

E

R

-

G

G

A

R

V

V

I

V

G

G

Y

Y

A

A

Y

Y

A

A

S

S

A

Q

F

H

R
|
R

N

A

R
|
R

T

A

A

A

Y
|
Y

R

R

F

W

L

A

V

V

E

D

D

V

S

T

I

V

Y

Y

L

V

S

A

P

E

E

G

A

Q

R

R

G

R

K

S

G

G

I

I

G

A

K

R

A

Q

L

L

L

Y

S

D

E

V

L

L

V

L

G

P

R

V

C

L

T

K

A

R

L

L

G

G

F

Y

R

R

Q

S

M

A

I

Y

A

A

V

G

I

I

G

A

G

L

A

P

H

N

P

E

S

G

S

S

I

V

A

G

L

L

H

H

R

E

A

R

L

L

G

G

F

F

E

Q

L

H

Q

I

G

G

L

T

M

F

K

P

A

Q

T

V

G

G

F

F

K

K

H

L

G

D

R

A

W

W

L

H

D

D

T

V

A

G

F

Y

M

W

Q

R

R

F

P

D

L

F

G

G

E

D

G

-

T

-

A

-

T

-

K

-

P

-

T

-

E

E

G

G

V

L

Y

H

P

P

D

E

binding (2S)-2-amino-4-[hydroxy(methyl)arsoryl]butanoic acid: S29 (≠ T30), F30 (≠ Y31), R72 (= R75), R74 (= R77), Y77 (= Y80)

6m7gA Crystal structure of arsn, n-acetyltransferase with substrate phosphinothricin from pseudomonas putida kt2440 (see paper)
37% identity, 95% coverage: 5:180/185 of query aligns to 4:170/176 of 6m7gA

query
sites
6m7gA

L

I

R

R

D

V

A

A

V

R

A

P

A

E

D

D

L

A

R

E

S

E

I

I

T

Q

E

I

I

I

Y

Y

R

A

E

P

S

I

V

V

L

L

N

N

G

T

V

A

A

I

T

S

Y

F

E

E

T

A

P

V

P

P

S

S

E

V

A

E

E

Q

M

M

A

R

L

E

R

R

F

I

S

S

T

T

I

-

T

T

G

L

N

Q

G

T

Y

Y

P

P

Y

Y

V

L

V

V

A

A

L

V

D

R

E

E

R

-

G

G

A

R

V

V

I

V

G

G

Y

Y

A

A

Y

Y

A

A

S

S

A

Q

F

H

R

R

N

A

R
|
R

T

A

A

A

Y
|
Y

R

R

F

W

L

A

V

V

E

D

D

V

S

T

I

V

Y

Y

L

V

S

A

P

E

E

G

A

Q

R

R

G

R

K

S

G

G

I

I

G

A

K

R

A

Q

L

L

L

Y

S

D

E

V

L

L

V

L

G

P

R

V

C

L

T

K

A

R

L

L

G

G

F

Y

R

R

Q

S

M

A

I

Y

A

A

V

G

I

I

G

A

G

L

A

P

H

N

P

E

S

G

S

S

I

V

A

G

L

L

H

H

R

E

A

R

L

L

G

G

F

F

E

Q

L

H

Q

I

G

G

L

T

M

F

K

P

A

Q

T

V

G

G

F

F

K

K

H

L

G

D

R

A

W

W

L

H

D

D

T

V

A

G

F

Y

M

W

Q

R

R

F

P

D

L

F

G

G

E

D

G

-

T

-

A

-

T

-

K

-

P

-

T

-

E

E

G

G

V

L

Y

H

P

P

D

E

binding phosphinothricin: R74 (= R77), Y77 (= Y80)

Query Sequence

>SMc03840 FitnessBrowser__Smeli:SMc03840
MTATLRDAVAADLRSITEIYRESVLNGVATYEETPPSEAEMALRFSTITGNGYPYVVALD
ERGAVIGYAYASAFRNRTAYRFLVEDSIYLSPEARGKGIGKALLSELVGRCTALGFRQMI
AVIGGAHPSSIALHRALGFELQGLMKATGFKHGRWLDTAFMQRPLGEGTATKPTEGVYPD
TLYRS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory