SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SMc03882 FitnessBrowser__Smeli:SMc03882 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4isdA Crystal structure of glutathione transferase homolog from burkholderia gl bgr1, target efi-501803, with bound glutathione
28% identity, 93% coverage: 4:204/215 of query aligns to 5:185/187 of 4isdA

query
sites
4isdA

L

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binding glutathione: Q43 (≠ F47), R51 (= R55), V52 (= V56), E64 (= E68), S65 (= S69)

O43708 Maleylacetoacetate isomerase; MAAI; GSTZ1-1; Glutathione S-transferase zeta 1; EC 5.2.1.2; EC 2.5.1.18 from Homo sapiens (Human) (see 10 papers)
32% identity, 52% coverage: 2:112/215 of query aligns to 4:115/216 of O43708

query
sites
O43708

A

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N

K32 (≠ E32) to E: in allele GSTZ1*C; dbSNP:rs7975
R42 (≠ A42) to G: in allele GSTZ1*B and allele GSTZ1*C; dbSNP:rs7972
Q45 (vs. gap) binding
V59 (= V56) binding
M82 (≠ L79) to T: in dbSNP:rs1046428
V99 (≠ A96) to M: in MAAID; decreased maleylacetoacetate isomerase activity
Q111 (≠ R108) binding

Sites not aligning to the query:

133 N → H: in dbSNP:rs2234955
150 A → V: in MAAID; decreased maleylacetoacetate isomerase activity

Q9WVL0 Maleylacetoacetate isomerase; MAAI; GSTZ1-1; Glutathione S-transferase zeta 1; EC 5.2.1.2; EC 2.5.1.18 from Mus musculus (Mouse)
34% identity, 52% coverage: 2:112/215 of query aligns to 4:115/216 of Q9WVL0

query
sites
Q9WVL0

A

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Q45 (≠ R46) binding
V59 (= V56) binding
Q111 (≠ R108) binding

3qawA Crystal structure of a glutathione-s-transferase from antarctic clam laternula elliptica in a complex with glutathione (see paper)
24% identity, 86% coverage: 2:185/215 of query aligns to 1:199/219 of 3qawA

query
sites
3qawA

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S

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active site: S11 (≠ N12)
binding glutathione: S11 (≠ N12), P13 (≠ S14), F35 (= F38), H40 (vs. gap), K41 (≠ G43), Q53 (≠ R55), V54 (= V56), E66 (= E68), S67 (= S69)

2cz2A Crystal structure of glutathione transferase zeta 1-1 (maleylacetoacetate isomerase) from mus musculus (form-1 crystal)
34% identity, 52% coverage: 2:112/215 of query aligns to 1:112/212 of 2cz2A

query
sites
2cz2A

A

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N

binding glutathione: S11 (≠ N12), C13 (≠ S14), L35 (≠ F40), Q42 (≠ R46), Q55 (≠ R55), V56 (= V56), Q68 (≠ E68), S69 (= S69), Q108 (≠ R108), N112 (≠ G112)

Sites not aligning to the query:

binding glutathione: 114

1fw1A Glutathione transferase zeta/maleylacetoacetate isomerase (see paper)
33% identity, 51% coverage: 3:112/215 of query aligns to 1:111/208 of 1fw1A

query
sites
1fw1A

K

K

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N

binding glutathione: S10 (≠ N12), C12 (≠ S14), L34 (≠ F38), Q41 (vs. gap), Q54 (≠ R55), V55 (= V56), Q67 (≠ E68), S68 (= S69), N111 (≠ G112)
binding sulfate ion: S11 (≠ Y13), Q107 (≠ R108), Q110 (≠ R111)

Sites not aligning to the query:

3qagA Human glutathione transferase o2 with glutathione -new crystal form (see paper)
25% identity, 89% coverage: 13:204/215 of query aligns to 30:207/238 of 3qagA

query
sites
3qagA

Y

F

S
x
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W
x
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K

binding glutathione: C31 (≠ S14), Y33 (≠ W16), K58 (≠ G43), H70 (≠ R55), I71 (≠ V56), E84 (= E68), S85 (= S69)

4xt0A Crystal structure of beta-etherase ligf from sphingobium sp. Strain syk-6 (see paper)
38% identity, 39% coverage: 17:100/215 of query aligns to 14:100/243 of 4xt0A

query
sites
4xt0A

S

S

L

L

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W

L

L

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E

D

L

V

F

F

P

P

D

E

A

S

G

G

-

N

-

S

L

L

W

R

P

P

E

A

D

D

R

P

A

F

D

K

R

R

A

A

R

E

A

M

R

R

S

V

Y

W

S

T

binding glutathione: Q40 (≠ A42), H41 (≠ G43), Q53 (≠ R55), V54 (= V56), E66 (= E68), S67 (= S69)

Sites not aligning to the query:

binding glutathione: 13, 149

P77544 Glutathione S-transferase YfcF; EC 2.5.1.18 from Escherichia coli (strain K12) (see paper)
27% identity, 85% coverage: 4:186/215 of query aligns to 6:197/214 of P77544

query
sites
P77544

L

I

V

T

L

L

Y

W

V
x
S

G

D

N

A

K

H

N

F

Y

F

S
|
S

S

P

W

Y

S

V

L

L

R

S

P

A

W

W

L

V

A

A

L

L

T

Q

A

E

G

K

G

G

I

L

P

S

F

F

E

H

D

I

V

K

V

T

V

I

P

D

F

L

D

D

F

S

A

G

A

E

G

H

-

L

N

Q

P

P

R

T

F

W

H

Q

E

G

I

Y

S

G

P

Q

T

T

G

R

R

R

V

V

P

P

V

L

L

L

H

Q

H

I

G

D

E

D

I

F

R

E

I

L

W

S

E

E

S

S

L

S

A

A

I

I

I

A

E

E

Y

Y

V

L

A

E

E

D

L

R

F

F

P

A

D

P

A

P

G

T

-

W

-

E

-

R

L

I

W

Y

P

P

E

L

D

D

R

L

A

E

D

N

R

R

A

A

R

R

A

A

R

R

S

Q

Y

I

S

Q

M

A

E

W

M

L

L

R

S

S

G

D

F

L

R

M

A

P

L

I

R

R

G

E

A

E

C

R

P

P

M

T

N

D

I

V

-

V

-

F

R

A

R

G

P

A

R

K

K

K

A

A

I

P

A

L

L

T

P

A

D

E

G

G

V

-

E

-

D

-

V

K

A

A

R

S

I

A

E

E

T

K

I

L

W

F

-

A

-

M

R

A

E

E

S

H

V

L

A

L

Q

V

S

L

G

G

G

Q

P

P

F

N

L

L

F

F

G

G

R

E

F

W

T

C

A

I

A

A

D

D

A

T

M

D

F

L

A

A

P

L

V

M

V

I

N

N

R

R

F

L

-

V

-

L

-

H

-

G

-

D

E

E

T

V

Y

P

E

E

L

R

V

L

A

V

D

D

P

Y

A

A

T

T

L

F

A

Q

Y

W

S10 (≠ V8) mutation to A: Nearly no effect on GSH transferase activity.
S16 (= S14) mutation to G: 2% of wild-type GSH transferase activity.

4qq7A Crystal structure of putative stringent starvation protein a from burkholderia cenocepacia with bound glutathione
27% identity, 90% coverage: 4:196/215 of query aligns to 3:191/204 of 4qq7A

query
sites
4qq7A

L

M

V

V

L

L

Y

Y

V

S

G

G

N

T

K

T

N

-

Y

-

S
x
C

S

P

W
x
F

S

S

L

Q

R

R

P

C

W

R

L

L

A

V

L

L

T

F

A

E

G

K

G

G

I

M

P

D

F

F

E

E

D

-

V

-

V

I

V

R

P

D

F

V

D

D

F

L

A

F

A

N

G
x
K

N

P

P

E

R

D

F

I

H

S

E

V

I

M

S

N

P

P

T

Y

G

G

R
x
Q

V
|
V

P

P

V

I

L

L

H

V

H

E

G

R

E

D

I

L

R

I

I

L

W

Y

E
|
E

S
|
S

L

N

A

I

I

I

I

N

E

E

Y

Y

V

I

A

D

E

E

L

R

F

F

P

P

D

H

A

P

G

Q

L

L

W

M

P

P

E

A

D

D

R

P

A

V

D

Q

R

R

A

A

R

R

A

A

R

R

-

L

-
x
F

-

L

S

N

Y
x
F

S

E

M

K

E

E

M

L

L

F

S

V

G

H

F

V

R

S

A

T

L

L

R
x
E

G
x
N

-

E

-

K

-

G

-

K

A

A

C

A

P

E

M

K

N

N

I

H

R

E

R

K

P

A

R

R

K

L

A

A

I

I

A

R

L

-

P

-

D

-

G

-

V

-

E

D

D

R

V

L

A

T

R

Q

I

L

E

A

T

P

I
|
I

W

F

R

V

E

K

S

N

V

K

A

Y

Q
x
M

S

L

G

G

G

-

P

-

F

-

L

-

F

-

G

E

R

E

F

F

T

S

A

M

A

L

D

D

A

V

M

A

F

I

A

A

P

P

V

L

N

W

R

R

F

L

E

D

T

H

Y

Y

-

G

-

I

E

E

L

L

V

S

A

K

D

N

P

A

A

A

T

P

L

L

-

M

A

K

Y

Y

I

A

D

E

A

R

V

I

K

F

A

S

H

R

P

P

A

A

F

Y

binding D-glucose: F94 (vs. gap), F98 (≠ Y99), I138 (= I141), M145 (≠ Q148)
binding glutathione: C11 (≠ S14), F13 (≠ W16), K38 (≠ G43), Q50 (≠ R55), V51 (= V56), E63 (= E68), S64 (= S69), E110 (≠ R111), N111 (≠ G112)

7aiaAAA Ganglioside-induced differentiation-associated protein 1 (see paper)
32% identity, 54% coverage: 2:117/215 of query aligns to 2:112/259 of 7aiaAAA

query
sites
7aiaAAA

A

V

K

K

L

L

V

I

L

L

Y

Y

V

H

G

W

N

T

K

H

N

S

Y

F

S

S

W

Q

S

K

L

V

R

R

P

L

W

V

L

I

A

A

L

E

T

K

A

A

G

L

G

K

I

C

P

E

F

E

E

H

D

D

V

V

V

S

V

L

P

P

F

L

D

S

F

-

A

E

A

H

G

N

N

E

P

P

R

W

F

F

H

M

E

R

I

L

S

N

P

S

T

T

G

G

R

E

V

V

P

P

V

V

L

L

H

I

H

H

G

G

E

E

I

N

R

I

I

I

W

C

E

E

S

A

L

T

A

Q

I

I

E

D

Y

Y

V

L

A

E

E

Q

L

T

F

F

P

L

D

D

A

-

G

-

L

-

W

-

P

-

E

-

D

E

R

R

A

T

D

P

R

R

A

L

R

M

A

P

R

D

S

K

Y

E

S

S

M

M

E

Y

M

Y

-

P

-

R

L

V

S

Q

G

H

F

Y

R

R

A

E

L

L

R

L

G

D

A

S

C

L

P

P

M

M

N

D

Sites not aligning to the query:

binding hexadecanedioic acid: 192, 239, 243, 244

Q8TB36 Ganglioside-induced differentiation-associated protein 1; GDAP1 from Homo sapiens (Human) (see 12 papers)
32% identity, 53% coverage: 3:117/215 of query aligns to 25:134/358 of Q8TB36

query
sites
Q8TB36

K

K

L

L

V

I

L

L

Y

Y

V

H

G

W

N

T

K

H

N

S

Y

F

S

S

W

Q

S
x
K

L

V

R

R

P

L

W

V

L

I

A

A

L

E

T

K

A

A

G

L

G
x
K

I

C

P

E

F

E

E

H

D

D

V

V

V

S

V

L

P

P

F

L

D

S

F

-

A

E

A

H

G

N

N

E

P

P

R

W

F

F

H

M

E

R

I

L

S

N

P

S

T

T

G

G

R

E

V

V

P

P

V

V

L

L

H

I

H

H

G

G

E

E

I

N

R

I

I

I

W

C

E

E

S

A

L

T

A

Q

I

I

E

D

Y

Y

V

L

A

E

E

Q

L

T

F

F

P

L

D

D

A

-

G

-

L

-

W

-

P

-

E

-

D

E

R

R

A

T

D

P

R

R

A

L

R

M

A

P

R

D

S

K

Y

E

S

S

M
|
M

E

Y

M

Y

-

P

-
x
R

L

V

S

Q

G
x
H

F

Y

R

R

A
x
E

L

L

R

L

G

D

A

S

C

L

P

P

M

M

N

D

K39 (≠ S17) to N: found in a patient with hereditary motor neuropathy; uncertain significance; dbSNP:rs1586794314
K50 (≠ G28) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
M116 (= M101) mutation to H: Impairment in the ability to induce mitochondrial fragmentation.
R120 (vs. gap) to G: in CMT2K; dominant form; dbSNP:rs104894078; to Q: in CMT4A; no effect on mitochondrial localization but impairment in the ability to induce mitochondrial fragmentation; dbSNP:rs1174933176; to W: in CMT2K; dominant form; no effect on mitochondrial localization; dbSNP:rs104894078
H123 (≠ G106) to R: in CMT2K; dominant form;; dbSNP:rs397515442
E126 (≠ A109) to K: in CMT2K; uncertain significance; dbSNP:rs879254005

Sites not aligning to the query:

156 A → G: in CMT2K; dominant form; dbSNP:rs397515441
157 T→P: No effect on mitochondrial localization.
161 R → H: in CMT4A; no effect on mitochondrial localization but abolishes mitochondrial fission; dbSNP:rs104894076
172 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
173 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
188 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
190 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
203 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate
206 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
207 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
214 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
218 Q → E: in CMT2K; uncertain significance; dbSNP:rs121908113
226 R → S: in CMT2K; uncertain significance; dbSNP:rs267606842
247 A → V: in CMT2K; uncertain significance; dbSNP:rs1586807209
256 H → R: in CMT2K; recessive form; dbSNP:rs1476856429
282 R → C: in CMTRIA; no effect on mitochondrial localization but impairment in the ability to induce mitochondrial fragmentation; dbSNP:rs28937906; R → H: in CMT2K; recessive form; dbSNP:rs375431837
310 R → Q: in CMT2RV; Abolishes mitochondrial fission; dbSNP:rs1323153568; R → W: in CMT2K; recessive form; dbSNP:rs538389475

4kdxA Crystal structure of a glutathione transferase family member from burkholderia graminis, target efi-507264, bound gsh, ordered domains, space group p21, form(1)
28% identity, 72% coverage: 45:199/215 of query aligns to 42:201/207 of 4kdxA

query
sites
4kdxA

P

P

R

E

F

F

H

L

E

A

I

L

S

N

P

P

T

N

G

A

R
x
M

V
|
V

P

P

V

V

L

I

H

R

H

D

G

G

E

D

I

F

R

V

I

L

W

W

E
|
E

S
|
S

L

N

A

S

I

I

E

R

Y

Y

V

L

A

A

E

G

L

R

F

Y

P

G

D

G

A

E

G

W

L

L

W

Y

P

P

E

A

D

D

R

A

A

R

D

E

R

R

A

A

R

R

A

C

-

D

-

Q

-

W

-

I

-

D

-

W

-

Q

-

A

-

S

-

E

-

L

-

N

R

R

S

S

Y

W

S

S

M

Y

E

A

M

F

L

L

S

A

G

L

F

V

R

R

-

Q

-

S

-

P

A

A

L

H

R

R

G

D

A

A

C

Q

P

Q

M

I

N

E

I

A

R

S

R

R

P

A

R

N

-

W

-

A

K

K

A

H

I

M

A

A

L

I

P

V

D

E

G

G

V

Q

V

L

E

-

D

-

V

-

A

-

R

-

I

-

E

-

T

-

I

-

W

-

R

-

E

-

S

-

V

-

A

-

Q

Q

S

R

G

T

G

G

P

A

F

F

L

I

F

A

G

G

R

D

-

A

F

F

T

S

A

L

A

A

D

D

A

I

M

P

F

I

A

A

P

L

V

S

V

I

N

N

R

R

F

W

E

L

T

E

Y

T

E

P

L

I

V

A

A

R

D

D

-

D

-

L

P

P

A

A

T

V

L

D

A

A

Y

Y

I

M

D

T

A

R

V

L

K

A

A

S

H

R

P

D

A

A

F

Y

R

R

Q

E

W

Y

binding glutathione: M52 (≠ R55), V53 (= V56), E65 (= E68), S66 (= S69)

Sites not aligning to the query:

active site: 10
binding glutathione: 10, 12, 33, 34

3uarA Crystal structure of glutathione transferase (target efi-501774) from methylococcus capsulatus str. Bath with gsh bound
27% identity, 84% coverage: 17:197/215 of query aligns to 12:194/203 of 3uarA

query
sites
3uarA

S

S

L

L

R

A

P

P

W

H

L

I

A

V

L

L

T

R

A

E

G

A

G

G

I

L

P

D

F

F

E

E

-

L

-

E

D

N

V

V

V

D

V

L

P

G

F

T

D

K

F

K

A

T

A

G

G

S

N

G

P

A

R

D

F

F

H

L

E

Q

I

V

S

N

P

P

T

K

G

G

R
x
Y

V
|
V

P

P

V

A

L

L

H

Q

H

L

G

D

E

D

I

G

R

Q

I

V

W

L

-

T

E
|
E

S
x
D

L

Q

A

V

I

I

I

L

E

Q

Y

Y

V

L

A

A

E

D

L

L

F

K

P

P

D

E

A

S

G

G

L

L

W

M

P

P

E

P

D

S

R

G

A

T

-

F

D

E

R

R

A

Y

R

R

A

L

R

L

S

E

Y

W

-

L

-

A

-

F

-

I

S

S

M

T

E

E

M

I

L

H

S
x
K

G

T

F

F

R

G

A

P

L

F

R

-

G

-

A

-

C

-

P

-

M

W

N

N

I

P

R

E

R

S

P

P

R

E

K

A

A

S

I

K

A

Q

L

I

P

A

D

L

G

G

V

L

E

S

D

-

V

-

A

R

R

R

I

L

E

D

T

Y

I

V

W

-

R

-

E

E

S

D

V

R

A

L

Q

E

S

A

G

G

P

P

F

W

L

L

F

M

G

G

-

D

R

R

F

Y

T

S

A

V

A

A

D

D

A

A

M

Y

F

L

A

S

P

T

V

V

V

L

N

G

-
x
W

-

C

R

E

F

Y

E

L

T

K

Y

I

E

D

L

L

V

S

A

K

D

W

P

P

A

R

T

I

L

L

A

A

Y

Y

I

L

D

E

A

R

V

N

K

Q

A

A

H

R

P

P

A

A

F

V

R

Q

binding glutathione: Y52 (≠ R55), V53 (= V56), E66 (= E68), D67 (≠ S69), K108 (≠ S105), W166 (vs. gap)

Sites not aligning to the query:

active site: 10
binding glutathione: 11

7dweA Crystal structure of a glutathione s-transferase sbgstu7 from salix babylonica in complex with glutathione
28% identity, 56% coverage: 14:134/215 of query aligns to 11:133/212 of 7dweA

query
sites
7dweA

S
|
S

S

P

W
x
F

S

S

L

H

R

R

P

I

W

I

L

W

A

A

L

L

T

K

A

L

G

K

G

G

I

V

P

E

F

Y

E

E

D

-

V

-

V

Y

V

I

P

E

F

E

D

D

F
x
L

A

S

A

N

G
x
K

N

S

P

E

R

S

F

L

H

L

E

K

I

Y

S

N

P

P

T

V

-

Y

G

K

R
x
K

V
x
T

P

P

V

V

L

L

H

V

H

H

G

G

E

D

I

K

R

P

I

I

W

A

E
|
E

S
|
S

L

L

A

V

I

I

I

L

E

E

Y

Y

V

I

A

E

E

E

L

T

F

W

P

P

D

E

A

N

G

P

L

L

W

L

P

P

E

K

D

D

R

P

A

Y

D

E

R

R

A

A

R

M

A

A

R

R

-

F

-

W

-

I

S

Q

Y

Y

S

G

M

V

E

D

M

T

L

V

S

A

G

A

F

L

R

R

A

A

L

F

R

Y

G

L

A

G

C

S

P

G

M

E

N

E

I

L

R

E

R

K

P

A

R

A

K

K

A

E

I

L

A

S

L

E

P

C

D

L

G

K

V

I

V

L

E

E

D

E

binding glutathione: S11 (= S14), F13 (≠ W16), L35 (≠ F40), K38 (≠ G43), K51 (≠ R55), T52 (≠ V56), E64 (= E68), S65 (= S69)

8a0pA Crystal structure of poplar glutathione transferase u20 in complex with morin (see paper)
32% identity, 52% coverage: 14:125/215 of query aligns to 11:124/216 of 8a0pA

query
sites
8a0pA

S
|
S

S
x
P

W
x
F

S

N

L

Y

R

R

P

V

W

I

L

W

A

A

L

L

T

K

A

L

G

K

G

G

I

V

P

E

F

F

E

E

D

H

V

I

V

V

V

E

P

-

F

-

D

D

F

L

A

T

A

N

G

K

N

S

P

E

R

L

F

L

H

L

E

K

I

Y

S

N

P

P

T

V

-

Y

G

K

R

K

V

I

P

P

V

V

L

L

H

V

H

H

G

G

E

G

I

K

R

P

I

I

W

A

E

E

S

S

L

L

A

V

I

I

I

L

E

E

Y

Y

V

I

A

E

E

E

L

T

F

W

P

P

D

E

A

N

G

P

L

L

W

L

P

P

E

T

D

D

R

P

A

Y

D

E

R

R

A

A

R

M

A

A

R

R

-

F

-

W

-

I

S

Q

Y

Y

S

G

M

A

E

T

M

K

L

T

S

A

G

A

F
|
F

R

G

A

A

L

L

R

F

G

R

A

A

C

S

P

G

M

E

N

E

I

L

R

E

R

K

P

A

R

A

K

K

A

E

I

V

binding 2-[2,4-bis(oxidanyl)phenyl]-3,5,7-tris(oxidanyl)chromen-4-one: S11 (= S14), P12 (≠ S15), F13 (≠ W16), F106 (= F107)

Sites not aligning to the query:

binding 2-[2,4-bis(oxidanyl)phenyl]-3,5,7-tris(oxidanyl)chromen-4-one: 156, 164, 207, 208

8a0rA Crystal structure of poplar glutathione transferase u20 in complex with pinocembrin (see paper)
32% identity, 53% coverage: 14:126/215 of query aligns to 10:124/215 of 8a0rA

query
sites
8a0rA

S

S

S
x
P

W
x
F

S

N

L

Y

R

R

P

V

W

I

L

W

A

A

L

L

T

K

A

L

G

K

G

G

I

V

P

E

F

F

E

E

D

H

V

I

V

V

V

E

P

-

F

-

D

D

F

L

A

T

A

N

G

K

N

S

P

E

R

L

F

L

H

L

E

K

I

Y

S

N

P

P

T

V

-

Y

G

K

R

K

V

I

P

P

V

V

L

L

H

V

H

H

G

G

E

G

I

K

R

P

I

I

W

A

E

E

S

S

L

L

A

V

I

I

I

L

E

E

Y

Y

V

I

A

E

E

E

L

T

F

W

P

P

D

E

A

N

G

P

L

L

W

L

P

P

E

T

D

D

R

P

A

Y

D

E

R

R

A

A

R

M

A

A

R

R

-

F

-

W

-

I

S

Q

Y

Y

S

G

M

A

E

T

M

K

L

T

S

A

G

A

F
|
F

R

G

A

A

L

L

R

F

G

R

A

A

C

S

P

G

M

E

N

E

I

L

R

E

R

K

P

A

R

A

K

K

A

E

I

V

A

V

binding pinocembrin: P11 (≠ S15), F12 (≠ W16), F105 (= F107)

Sites not aligning to the query:

binding pinocembrin: 155, 163

8a0qA Crystal structure of poplar glutathione transferase u20 in complex with baicalein (see paper)
32% identity, 53% coverage: 14:126/215 of query aligns to 10:124/215 of 8a0qA

query
sites
8a0qA

S

S

S
x
P

W
x
F

S

N

L

Y

R

R

P

V

W

I

L

W

A

A

L

L

T

K

A

L

G

K

G

G

I

V

P

E

F

F

E

E

D

H

V

I

V

V

V

E

P

-

F

-

D

D

F

L

A

T

A

N

G

K

N

S

P

E

R

L

F

L

H

L

E

K

I

Y

S

N

P

P

T

V

-

Y

G

K

R

K

V

I

P

P

V

V

L

L

H

V

H

H

G

G

E

G

I

K

R

P

I

I

W

A

E

E

S

S

L

L

A

V

I

I

I

L

E

E

Y

Y

V

I

A

E

E

E

L

T

F

W

P

P

D

E

A

N

G

P

L

L

W

L

P

P

E

T

D

D

R

P

A

Y

D

E

R

R

A

A

R

M

A

A

R

R

-

F

-

W

-

I

S

Q

Y

Y

S

G

M

A

E

T

M

K

L

T

S

A

G

A

F
|
F

R

G

A

A

L

L

R

F

G

R

A

A

C

S

P

G

M

E

N

E

I

L

R

E

R

K

P

A

R

A

K

K

A

E

I

V

A

V

binding 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one: P11 (≠ S15), F12 (≠ W16), F105 (= F107)

Sites not aligning to the query:

binding 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one: 155, 159, 163

8a0oA Crystal structure of poplar glutathione transferase u20 in complex with galangin (see paper)
32% identity, 53% coverage: 14:126/215 of query aligns to 10:124/215 of 8a0oA

query
sites
8a0oA

S
|
S

S
x
P

W
x
F

S

N

L

Y

R

R

P

V

W

I

L

W

A

A

L

L

T

K

A

L

G

K

G

G

I

V

P

E

F

F

E

E

D

H

V

I

V

V

V

E

P

-

F

-

D

D

F

L

A

T

A

N

G

K

N

S

P

E

R

L

F

L

H

L

E

K

I

Y

S

N

P

P

T

V

-

Y

G

K

R

K

V

I

P

P

V

V

L

L

H

V

H

H

G

G

E

G

I

K

R

P

I

I

W

A

E

E

S

S

L

L

A

V

I

I

I

L

E

E

Y

Y

V

I

A

E

E

E

L

T

F

W

P

P

D

E

A

N

G

P

L

L

W

L

P

P

E

T

D

D

R

P

A

Y

D

E

R

R

A

A

R

M

A

A

R

R

-

F

-

W

-

I

S

Q

Y

Y

S

G

M

A

E

T

M

K

L
x
T

S

A

G

A

F

F

R

G

A

A

L

L

R

F

G

R

A

A

C

S

P

G

M

E

N

E

I

L

R

E

R

K

P

A

R

A

K

K

A

E

I

V

A

V

binding galangin: S10 (= S14), P11 (≠ S15), F12 (≠ W16), T102 (≠ L104)

Sites not aligning to the query:

binding galangin: 9, 155, 160, 163, 206

8a0iA Crystal structure of poplar glutathione transferase u20 in complex with glutathionylphenylacetophenone (see paper)
32% identity, 53% coverage: 14:126/215 of query aligns to 10:124/215 of 8a0iA

query
sites
8a0iA

S
|
S

S

P

W
x
F

S

N

L

Y

R

R

P

V

W

I

L

W

A

A

L

L

T

K

A

L

G

K

G

G

I

V

P

E

F

F

E

E

D

H

V

I

V

V

V

E

P

-

F

-

D

D

F
x
L

A

T

A

N

G

K

N

S

P

E

R

L

F

L

H

L

E

K

I

Y

S

N

P

P

T

V

-

Y

G

K

R
x
K

V
x
I

P

P

V

V

L

L

H

V

H

H

G

G

E

G

I

K

R

P

I

I

W

A

E
|
E

S
|
S

L

L

A

V

I

I

I

L

E

E

Y

Y

V

I

A

E

E

E

L

T

F

W

P

P

D

E

A

N

G

P

L

L

W

L

P

P

E

T

D

D

R

P

A

Y

D

E

R

R

A

A

R

M

A

A

R

R

-

F

-

W

-

I

S

Q

Y

Y

S

G

M

A

E

T

M

K

L

T

S

A

G

A

F

F

R
x
G

A

A

L

L

R
x
F

G

R

A

A

C

S

P

G

M

E

N

E

I

L

R

E

R

K

P

A

R

A

K

K

A

E

I

V

A

V

binding L-gamma-glutamyl-S-(2-biphenyl-4-yl-2-oxoethyl)-L-cysteinylglycine: S10 (= S14), F12 (≠ W16), L34 (≠ F40), K50 (≠ R55), I51 (≠ V56), E63 (= E68), S64 (= S69), G106 (≠ R108), F109 (≠ R111)

Sites not aligning to the query:

binding L-gamma-glutamyl-S-(2-biphenyl-4-yl-2-oxoethyl)-L-cysteinylglycine: 163, 207, 214

Query Sequence

>SMc03882 FitnessBrowser__Smeli:SMc03882
MAKLVLYVGNKNYSSWSLRPWLALTAGGIPFEDVVVPFDFAAGNPRFHEISPTGRVPVLH
HGEIRIWESLAIIEYVAELFPDAGLWPEDRADRARARSYSMEMLSGFRALRGACPMNIRR
PRKAIALPDGVVEDVARIETIWRESVAQSGGPFLFGRFTAADAMFAPVVNRFETYELVAD
PATLAYIDAVKAHPAFRQWEEAARVETWIVPEDEA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory