SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SMc04386 FitnessBrowser__Smeli:SMc04386 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P58350 Aspartate aminotransferase; AAT; AspAT; Putative 2-aminoadipate transaminase; Transaminase A; EC 2.6.1.1; EC 2.6.1.39 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
100% identity, 100% coverage: 1:410/410 of query aligns to 1:410/410 of P58350

query
sites
P58350

M

M

T

T

I

I

N

N

A

A

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T

V

V

K

K

E

E

A

A

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G

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F

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Q

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R

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G

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K

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E

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A

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G

E

E

P

P

D

D

F

F

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P

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E

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H

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V

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K

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A

A

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D

A

A

I

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H

H

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R

G

G

E

E

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K

K

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A

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L

D

D

G

G

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T

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P

E

E

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L

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K

K

K

A

A

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I

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R

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E

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K

F

F

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L

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Y

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E

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L

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V

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A

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G

A

A

K

K

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Q

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L

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F

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M

M

M

A

A

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D

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P

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D

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E

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E

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G

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K

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G

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A

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D

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Y

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R

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P

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H

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K
|
K

A

A

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T

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G

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W

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G

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P

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Q

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V

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D

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D

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S

K249 (= K249) modified: N6-(pyridoxal phosphate)lysine

6f35A Crystal structure of the aspartate aminotranferase from rhizobium meliloti (see paper)
100% identity, 98% coverage: 11:410/410 of query aligns to 1:400/400 of 6f35A

query
sites
6f35A

G

G

F

F

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Q

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P

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A

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E

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G

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K

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M

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S

binding pyridoxal-5'-phosphate: G99 (= G109), A100 (= A110), K101 (= K111), D204 (= D214), M206 (= M216), Y207 (= Y217), S238 (= S248), K239 (= K249), R247 (= R257)

6f77A Crystal structure of the prephenate aminotransferase from rhizobium meliloti (see paper)
59% identity, 96% coverage: 15:406/410 of query aligns to 4:395/399 of 6f77A

query
sites
6f77A

A

A

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A

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E

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K

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G

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E

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D

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F

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P

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K

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K

A

A

A

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I

D

D

A

A

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D

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R

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G

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K

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G

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E

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K

E

A

A

I

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E

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K

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E

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Y

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G
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G

A
x
G

K
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K

Q

Q

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I

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L

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F

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N

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F

M

M

A

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P

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G

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D

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A

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Y

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E

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M

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A

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E

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T

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P

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P

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S

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S

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G

A

A

A

A

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Y

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S

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H

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H

H

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V

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W

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S

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K

A

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Y

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M

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T

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G

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Q

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D

L

L

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M

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L

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A

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K

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T

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P

E

E

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G

A

A

F

F

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G

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C

C

A

A

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G

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H

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N

F

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Y

A

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E

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A

A

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E

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E

A

A

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C

E

R

R

R

I

I

A

Q

A

R

A

F

C

C

binding pyridoxal-5'-phosphate: G98 (= G109), G99 (≠ A110), K100 (= K111), W124 (= W135), N170 (= N181), N174 (= N185), D203 (= D214), Y206 (= Y217), S237 (= S248), K238 (= K249), R246 (= R257)

Q02635 Aspartate/prephenate aminotransferase; AspAT / PAT; Transaminase A; EC 2.6.1.1; EC 2.6.1.79 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
59% identity, 96% coverage: 15:406/410 of query aligns to 5:396/400 of Q02635

query
sites
Q02635

A

A

S

D

R

A

I

L

S

S

S

R

I

V

G
x
K

V

P

S

S

E

A

I

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K

A

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K

A

A

A

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E

M

L

K

K

R

A

E

K

G

G

K

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D

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V

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I

I

G

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L

G

G

A

A

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G

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E

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P

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D

F

F

D

D

T

T

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P

E

D

H

N

V

I

K

K

Q

K

A

A

A

A

S

I

D

D

A

A

I

I

H

D

R

R

G

G

E

E

T

T

K

K

Y

Y

T

T

A

P

L

V

D

S

G

G

T

I

P

P

E

E

L

L

K

R

K

E

A

A

I

I

R

A

E

K

K

K

F

F

Q

K

R

R

E

E

N

N

G

N

L

L

A

D

Y

Y

E

T

L

A

D

A

E

Q

I

T

T

I

V

V

A

G

T

T

G

G

A

G

K

K

Q

Q

I

I

L

L

F

F

N

N

A

A

M

F

M

M

A

A

S

T

L

L

D

N

P

P

G

G

D

D

E

E

V

V

I

V

I

I

P

P

T

A

P

P

Y

Y

W

W

T

V

S

S

Y

Y

S

P

D

E

I

M

V

V

H

A

I

L

C

C

E

G

G

G

K

T

P

P

V

V

L

F

I

V

A

P

C

T

D

R

A

Q

S

E

S

N

G

N

F

F

R

K

L

L

T

K

A

A

E

E

K

D

L

L

E

D

A

R

A

A

I

I

T

T

P

P

R

K

T

T

R

K

W

W

V

F

L

V

L

F

N

N

S

S

P

P

S

S

N

N

P

P

S

S

G

G

A

A

A

A

Y

Y

S

S

A

H

A

E

D

E

Y

L

R

K

P

A

L

L

L

T

E

D

V

V

L

L

L

M

R

K

H

H

P

P

H

H

V

V

W

W

L

V

L

L

V

T

D

D

D

D

M

M

Y

Y

E

E

H

H

I

L

V

T

Y

Y

D

G

G

D

F

F

R

R

F

F

V

A

T

T

P

P

A

V

Q

E

L

V

E

E

P

P

G

G

L

L

K

Y

N

E

R

R

T

T

L

L

T

T

V

M

N

N

G

G

V

V

S

S

K

K

A

A

Y

Y

A

A

M

M

T

T

G

G

W

W

R

R

I

I

G

G

Y

Y

A

A

G

A

G

G

P

P

R

L

E

H

L

L

I

I

K

K

A

A

M

M

A

D

V

M

V

I

Q

Q

S

G

Q

Q

A

Q

T

T

S

S

C

G

P

A

S

A

S

S

I

I

S

A

Q

Q

A

W

A

A

S

A

V

V

A

E

A

A

L

L

N

N

G

G

P

P

Q

Q

D

D

F

F

L

I

K

G

E

R

R

N

T

K

E

E

S

I

F

F

Q

Q

R

G

R

R

R

R

D

D

L

L

V

V

V

V

N

S

G

M

L

L

N

N

A

Q

I

A

D

K

G

G

L

I

D

S

C

C

R

P

V

T

P

P

E

E

G

G

A

A

F

F

Y

Y

T

V

F

Y

S

P

G

S

C

C

A

A

G

G

V

L

L

I

G

G

K

K

V

T

T

A

P

P

S

S

G

G

K

K

R

V

I

I

K

E

T

T

D

D

T

E

D

D

F

F

C

V

A

S

Y

E

L

L

L

L

E

E

D

T

A

E

H

G

V

V

A

A

V

V

V

V

P

H

G

G

S

S

A

A

F

F

G

G

L

L

S

G

P

P

F

N

F

F

R

R

I

I

S

S

Y

Y

A

A

T

T

S

S

E

E

A

A

E

L

L

L

K

E

E

E

A

A

L

C

E

R

R

R

I

I

A

Q

A

R

A

F

C

C

K12 (≠ G22) Important for prephenate aminotransferase activity; mutation to G: 7-fold increase in Km for prephenate. Weakly affects Km for oxaloacetate.

8wkjA The crystal structure of aspartate aminotransferases lpg0070 from legionella pneumophila (see paper)
49% identity, 90% coverage: 40:406/410 of query aligns to 30:390/391 of 8wkjA

query
sites
8wkjA

G

G

K

L

P

D

V

I

I

I

I

G

L

L

G

G

A

T

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

E

Q

H

H

V

I

K

K

Q

L

A

A

A

A

S

I

D

S

A

A

I

I

H

E

R

A

G

G

E

D

T

T

K

K

Y

Y

T

T

A

A

L

V

D

D

G

G

T

I

P

V

E

E

L

L

K

K

K

E

A

A

I

V

R

K

E

N

K

K

F

F

Q

K

R

R

E

D

N

N

G

E

L

L

A

D

Y

Y

E

Q

L

L

D

N

E

Q

I

I

T

L

V

V

A

S

T

V

G
|
G

A
x
G

K
|
K

Q

Q

I

S

L

C

F

Y

N

N

A

L

M

C

M

Q

A

A

S

Y

L

L

D

N

P

P

G

G

D

D

E

E

V

V

I

I

I

I

P

P

T

A

P

P

Y

Y

W
|
W

T

V

S

S

Y

Y

S

P

D

D

I

M

V

V

H

L

I

L

C

A

E

D

G

G

K

V

P

P

V

V

L

I

I

I

A

E

C

T

D

T

A

P

S

A

S

Q

G

R

F

Y

R

K

L

I

T

N

A

A

E

Q

K

Q

L

L

E

E

A

Q

A

A

I

I

T

T

P

P

R

K

T

T

R

R

W

M

V

I

L

F

L

L

N
|
N

S

S

P

P

S

S

N
|
N

P

P

S

S

G

G

A

I

A

A

Y

Y

S

T

A

Q

A

N

D

E

Y

L

R

K

P

E

L

L

L

G

E

D

V

V

L

L

L

K

R

K

H

H

P

P

H

Q

V

I

W

L

L

I

L

A

V

T

D
|
D

D

D

M

M

Y
|
Y

E

E

H

H

I

I

V

I

Y

W

D

S

G

Q

F

-

R

P

F

F

V

T

T

N

P

I

A

L

Q

N

L

A

E

C

P

P

G

E

L

L

K

Y

N

D

R

R

T

T

L

I

T

V

V

L

N

N

G

G

V

V

S
|
S

K
|
K

A

A

Y

Y

A

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

A

A

G

A

G

G

P

P

R

A

E

P

L

L

I

I

K

N

A

A

M

M

A

K

V

T

V

I

Q

Q

S

S

Q

Q

A

S

T

T

S

S

C

N

P

P

S

C

S

S

I

I

S

A

Q

Q

A

R

A

A

S

A

V

V

A

A

A

A

L

L

N

N

G

G

P

S

Q

N

D

E

F

S

L

I

K

E

E

E

R

M

T

V

E

N

S

A

F

F

Q

H

R

Q

R

R

R

H

D

D

L

Y

V

V

V

A

N

D

G

R

L

L

N

Q

A

S

I

I

D

D

G

G

L

I

D

E

C

V

R

I

V

P

P

A

E

D

G

G

A

T

F

F

Y

Y

T

I

F

F

S

P

G

S

C

V

A

Q

G

A

V

I

L

I

G

E

K

K

V

-

T

-

P

-

S

-

G

-

K

R

R

G

I

Y

K

A

T

N

D

D

T

I

D

E

F

F

C

S

A

E

Y

K

L

L

L

L

E

N

D

E

A

V

H

G

V

V

A

A

V

L

V

V

P

P

G

G

S

S

A

A

F

F

G

G

L

T

S

E

P

G

F

C

F

I

R

R

I

I

S

S

Y

F

A

A

T

T

S

G

E

I

A

D

E

T

L

L

K

K

E

D

A

A

L

L

E

N

R

R

I

L

A

Q

A

R

A

F

C

C

binding pyridoxal-5'-phosphate: G99 (= G109), G100 (≠ A110), K101 (= K111), W125 (= W135), N171 (= N181), N175 (= N185), D204 (= D214), Y207 (= Y217), S237 (= S248), K238 (= K249), R246 (= R257)

1j32A Aspartate aminotransferase from phormidium lapideum
40% identity, 95% coverage: 15:402/410 of query aligns to 4:380/388 of 1j32A

query
sites
1j32A

A

A

S

A

R

R

I

V

S

E

S

S

I

V

G

S

V

P

S

S

E

M

I

T

L

L

K

I

I

I

G

D

A

A

R

K

A

A

A

K

A

A

M

M

K

K

R

A

E

E

G

G

K

I

P

D

V

V

I

C

I

S

L

F

G

S

A

A

G

G

E

E

P

P

D

D

F

F

D

N

T

T

P

P

E

K

H

H

V

I

K

V

Q

E

A

A

A

A

S

K

D

A

A

A

I

L

H

E

R

Q

G

G

E

K

T

T

K

R

Y

Y

T

G

A

P

L

A

D

A

G

G

T

E

P

P

E

R

L

L

K

R

K

E

A

A

I

I

R

A

E

Q

K

K

F

L

Q

Q

R

R

E

D

N

N

G

G

L

L

A

C

Y

Y

E

G

L

A

D

D

E

N

I

I

T

L

V

V

A

T

T

N

G
|
G

A
x
G

K
|
K

Q

Q

I

S

L

I

F

F

N

N

A

L

M

M

M

L

A

A

S

M

L

I

D

E

P

P

G

G

D

D

E

E

V

V

I

I

I

I

P

P

T

A

P

P

Y

F

W
|
W

T

V

S

S

Y

Y

S

P

D

E

I

M

V

V

H

K

I

L

C

A

E

E

G

G

K

T

P

P

V

V

L

I

I

L

A

P

C

T

D

T

A

V

S

E

S

T

G

Q

F

F

R

K

L

V

T

S

A

P

E

E

K

Q

L

I

E

R

A

Q

A

A

I

I

T

T

P

P

R

K

T

T

R

K

W

L

V

L

L

V

L

F

N

N

S

T

P

P

S

S

N
|
N

P

P

S

T

G

G

A

M

A

V

Y

Y

S

T

A

P

A

D

D

E

Y

V

R

R

P

A

L

I

L

A

E

Q

V

V

L

A

L

V

R

-

H

E

P

A

H

G

V

L

W

W

L

V

L

L

V

S

D
|
D

D

E

M
x
I

Y
|
Y

E

E

H

K

I

I

V

L

Y

Y

D

D

G

D

F

A

R

Q

F

H

V

L

T

S

P

I

A

G

Q

A

L

A

E

S

P

P

G

E

L

A

K

Y

N

E

R

R

T

S

L

V

T

V

V

C

N

S

G

G

V

F

S

A

K
|
K

A

T

Y

Y

A

A

M

M

T

T

G

G

W

W

R
|
R

I

V

G

G

Y

F

A

L

G

A

G

G

P

P

R

V

E

P

L

L

I

V

K

K

A

A

M

A

A

T

V

K

V

I

Q

Q

S

G

Q

H

A

S

T

T

S

S

C

N

P

V

S

C

S

T

I

F

S

A

Q

Q

A

Y

A

G

S

A

V

I

A

A

A

A

L

Y

N

E

G

N

P

S

Q

Q

D

D

F

C

L

V

K

Q

E

E

R

M

T

L

E

A

S

A

F

F

Q

A

R

E

R

R

R

R

D

R

L

Y

V

M

V

L

N

D

G

A

L

L

N

N

A

A

I

M

D

P

G

G

L

L

D

E

C

C

R

P

V

K

P

P

E

D

G

G

A

A

F

F

Y

Y

T

M

F

F

S

P

G

S

C

I

A

A

G

-

V

-

L

-

G

-

K

-

V

-

T

-

P

-

S

-

G

-

K

K

R

T

I

G

K

R

T

S

D

S

T

L

D

D

F

F

C

C

A

S

Y

E

L

L

L

L

E

D

D

Q

A

H

H

Q

V

V

A

A

V

T

V

V

P

P

G

G

S

A

A

A

F

F

G

G

L

A

S

D

P

D

F

C

F

I

R

R

I

L

S

S

Y

Y

A

A

T

T

S

D

E

L

A

D

E

T

L

I

K

K

E

R

A

G

L

M

E

E

R

R

I

L

active site: W124 (= W135), D202 (= D214), I204 (≠ M216), K237 (= K249)
binding pyridoxal-5'-phosphate: G98 (= G109), G99 (≠ A110), K100 (= K111), W124 (= W135), N174 (= N185), D202 (= D214), I204 (≠ M216), Y205 (= Y217), K237 (= K249), R245 (= R257)

Q56232 Aspartate/prephenate aminotransferase; AspAT / PAT; Transaminase A; EC 2.6.1.1; EC 2.6.1.78 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see 3 papers)
45% identity, 96% coverage: 17:408/410 of query aligns to 7:384/385 of Q56232

query
sites
Q56232

R

R

I

V

S

Q

S

A

I

M

G
x
K

V

P

S

S

E

A

I

T

L

V

K

A

I

V

G

N

A

A

R

K

A

A

A

L

A

E

M

L

K

R

R

R

E

Q

G

G

K

V

P

D

V

L

I

V

I

A

L

L

G

T

A

A

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

E

E

H

H

V

V

K

K

Q

E

A

A

A

A

S

R

D

R

A

A

I

L

H

A

R

Q

G

G

E

K

T

T

K

K

Y

Y

T

A

A

P

L

P

D

A

G

G

T

I

P

P

E

E

L

L

K

R

K

E

A

A

I

L

R

A

E

E

K

K

F

F

Q

R

R

R

E

E

N

N

G

G

L

L

A

S

Y

V

E

T

L

P

D

E

E

E

I

T

T

I

V

V

A

T

T

V

G

G

A

G

K

K

Q

Q

I

A

L

L

F

F

N

N

A

L

M

F

M

Q

A

A

S

I

L

L

D

D

P

P

G

G

D

D

E

E

V

V

I

I

I

V

P

L

T

S

P

P

Y

Y

W

W

T

V

S

S

Y

Y

S

P

D

E

I

M

V

V

H

R

I

F

C

A

E

G

G

G

K

V

P

V

V

V

L

E

I

V

A

E

C

T

D

L

A

P

S

E

S

E

G

G

F

F

R

V

L

P

T

D

A

P

E

E

K

R

L

V

E

R

A

R

A

A

I

I

T

T

P

P

R

R

T

T

R

K

W

A

V

L

L

V

L

V

N

N

S

S

P

P

S

N

N

N

P

P

S

T

G

G

A

A

A

V

Y

Y

S

P

A

K

A

E

D

V

Y

L

R

E

P

A

L

L

L

A

E

R

V

L

L

A

L

V

R

E

H

H

P

-

H

D

V

F

W

Y

L

L

L

V

V

S

D

D

D

E

M

I

Y

Y

E

E

H

H

I

L

V

L

Y

Y

D

E

G

G

F

E

R

H

F

F

V

-

T

S

P

P

A

G

Q

R

L

V

E

A

P

P

G

-

L

-

K

-

N

E

R

H

T

T

L

L

T

T

V

V

N

N

G

G

V

A

S

A

K
|
K

A

A

Y

F

A

A

M

M

T

T

G

G

W

W

R

R

I

I

G

G

Y

Y

A

A

G

C

G

G

P

P

R

K

E

E

L

V

I

I

K

K

A

A

M

M

A

A

V

S

V

V

Q

S

S

S

Q

Q

A

S

T

T

S

T

C

S

P

P

S

D

S

T

I

I

S

A

Q

Q

A

W

A

A

S

T

V

L

A

E

A

A

L

L

N

T

G

N

P

Q

Q

E

-

A

-

S

-

R

D

A

F

F

L

V

K

E

E

M

R

A

T

R

E

E

S

A

F

Y

Q

R

R

R

R

R

R

R

D

D

L

L

V

L

V

L

N

E

G

G

L

L

N

T

A

A

I

L

D

-

G

G

L

L

D

K

C

A

R

V

V

R

P

P

E

S

G

G

A

A

F

F

Y

Y

T

-

F

-

S

-

G

-

C

-

A

-

G

-

V

V

L

L

G

M

K

D

V

T

T

S

P

P

S

-

G

-

K

-

R

-

I

I

K

A

T

P

D

D

T

E

D

V

F

R

C

A

A

A

Y

E

L

R

L

L

E

L

D

E

A

A

H

G

V

V

A

A

V

V

V

V

P

P

G

G

S

T

A

D

F

F

G

A

L

A

S

F

P

G

F

H

F

V

R

R

I

L

S

S

Y

Y

A

A

T

T

S

S

E

E

A

E

E

N

L

L

K

R

E

K

A

A

L

L

E

E

R

R

I

F

A

A

A

R

A

V

C

L

D

G

R

R

K12 (≠ G22) Important for prephenate aminotransferase activity; mutation to G: 10-fold increase in Km for prephenate. Does not affect Km for oxaloacetate.
K234 (= K249) modified: N6-(pyridoxal phosphate)lysine

1bkgA Aspartate aminotransferase from thermus thermophilus with maleate (see paper)
45% identity, 94% coverage: 17:403/410 of query aligns to 7:379/382 of 1bkgA

query
sites
1bkgA

R

R

I

V

S

Q

S

A

I

M

G

K

V

P

S

S

E

A

I

T

L

V

K

A

I

V

G

N

A

A

R

K

A

A

A

L

A

E

M

L

K

R

R

R

E

Q

G

G

K

V

P

D

V

L

I

V

I

A

L

L

G

T

A

A

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

E

E

H

H

V

V

K

K

Q

E

A

A

A

A

S

R

D

R

A

A

I

L

H

A

R

Q

G

G

E

K

T

T

K

K

Y

Y

T

A

A

P

L

P

D

A

G

G

T

I

P

P

E

E

L

L

K

R

K

E

A

A

I

L

R

A

E

E

K

K

F

F

Q

R

R

R

E

E

N

N

G

G

L

L

A

S

Y

V

E

T

L

P

D

E

E

E

I

T

T

I

V

V

A

T

T

V

G
|
G

A
x
G

K
|
K

Q

Q

I

A

L

L

F

F

N

N

A

L

M

F

M

Q

A

A

S

I

L

L

D

D

P

P

G

G

D

D

E

E

V

V

I

I

I

V

P

L

T

S

P

P

Y

Y

W
|
W

T

V

S

S

Y

Y

S

P

D

E

I

M

V

V

H

R

I

F

C

A

E

G

G

G

K

V

P

V

V

V

L

E

I

V

A

E

C

T

D

L

A

P

S

E

S

E

G

G

F

F

R

V

L

P

T

D

A

P

E

E

K

R

L

V

E

R

A

R

A

A

I

I

T

T

P

P

R

R

T

T

R

K

W

A

V

L

L

V

L

V

N
|
N

S

S

P

P

S

N

N
|
N

P

P

S

T

G

G

A

A

A

V

Y

Y

S

P

A

K

A

E

D

V

Y

L

R

E

P

A

L

L

L

A

E

R

V

L

L

A

L

V

R

E

H

H

P

-

H

D

V

F

W

Y

L

L

L

V

V

S

D
|
D

D

E

M
x
I

Y
|
Y

E

E

H

H

I

L

V

L

Y

Y

D

E

G

G

F

E

R

H

F

F

V

-

T

S

P

P

A

G

Q

R

L

V

E

A

P

P

G

-

L

-

K

-

N

E

R

H

T

T

L

L

T

T

V

V

N

N

G

G

V

A

S

A

K
|
K

A

A

Y

F

A

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

A

A

G

C

G

G

P

P

R

K

E

E

L

V

I

I

K

K

A

A

M

M

A

A

V

S

V

V

Q

S

S

S

Q

Q

A

S

T

T

S

T

C

S

P

P

S

D

S

T

I

I

S

A

Q

Q

A

W

A

A

S

T

V

L

A

E

A

A

L

L

N

T

G

N

P

Q

Q

E

-

A

-

S

-

R

D

A

F

F

L

V

K

E

E

M

R

A

T

R

E

E

S

A

F

Y

Q

R

R

R

R

R

R

R

D

D

L

L

V

L

V

L

N

E

G

G

L

L

N

T

A

A

I

L

D

-

G

G

L

L

D

K

C

A

R

V

V

R

P

P

E

S

G

G

A

A

F

F

Y

Y

T

-

F

-

S

-

G

-

C

-

A

-

G

-

V

V

L

L

G

M

K

D

V

T

T

S

P

P

S

-

G

-

K

-

R

-

I

I

K

A

T

P

D

D

T

E

D

V

F

R

C

A

A

A

Y

E

L

R

L

L

E

L

D

E

A

A

H

G

V

V

A

A

V

V

V

V

P

P

G

G

S

T

A

D

F

F

G

A

L

A

S

F

P

G

F

H

F

V

R

R

I

L

S

S

Y

Y

A

A

T

T

S

S

E

E

A

E

E

N

L

L

K

R

E

K

A

A

L

L

E

E

R

R

I

F

A

A

active site: W125 (= W135), D203 (= D214), I205 (≠ M216), K234 (= K249)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G99 (= G109), G100 (≠ A110), K101 (= K111), W125 (= W135), N171 (= N181), N175 (= N185), D203 (= D214), I205 (≠ M216), Y206 (= Y217), K234 (= K249), R242 (= R257)

1bjwA Aspartate aminotransferase from thermus thermophilus (see paper)
45% identity, 94% coverage: 17:403/410 of query aligns to 7:379/382 of 1bjwA

query
sites
1bjwA

R

R

I

V

S

Q

S

A

I

M

G

K

V

P

S

S

E

A

I

T

L

V

K

A

I

V

G

N

A

A

R

K

A

A

A

L

A

E

M

L

K

R

R

R

E

Q

G

G

K

V

P

D

V

L

I

V

I

A

L

L

G

T

A

A

G
|
G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

E

E

H

H

V

V

K

K

Q

E

A

A

A

A

S

R

D

R

A

A

I

L

H

A

R

Q

G

G

E

K

T

T

K

K

Y

Y

T

A

A

P

L

P

D

A

G

G

T

I

P

P

E

E

L

L

K

R

K

E

A

A

I

L

R

A

E

E

K

K

F

F

Q

R

R

R

E

E

N

N

G

G

L

L

A

S

Y

V

E

T

L

P

D

E

E

E

I

T

T

I

V

V

A

T

T

V

G

G

A

G

K

K

Q

Q

I

A

L

L

F

F

N

N

A

L

M

F

M

Q

A

A

S

I

L

L

D

D

P

P

G

G

D

D

E

E

V

V

I

I

I

V

P

L

T

S

P

P

Y

Y

W
|
W

T

V

S

S

Y

Y

S

P

D

E

I

M

V

V

H

R

I

F

C

A

E

G

G

G

K

V

P

V

V

V

L

E

I

V

A

E

C

T

D

L

A

P

S

E

S

E

G

G

F

F

R

V

L

P

T

D

A

P

E

E

K

R

L

V

E

R

A

R

A

A

I

I

T

T

P

P

R

R

T

T

R

K

W

A

V

L

L

V

L

V

N

N

S

S

P

P

S

N

N
|
N

P

P

S

T

G

G

A

A

A

V

Y

Y

S

P

A

K

A

E

D

V

Y

L

R

E

P

A

L

L

L

A

E

R

V

L

L

A

L

V

R

E

H

H

P

-

H

D

V

F

W

Y

L

L

L

V

V

S

D
|
D

D

E

M
x
I

Y

Y

E

E

H

H

I

L

V

L

Y

Y

D

E

G

G

F

E

R

H

F

F

V

-

T

S

P

P

A

G

Q

R

L

V

E

A

P

P

G

-

L

-

K

-

N

E

R

H

T

T

L

L

T

T

V

V

N

N

G

G

V

A

S

A

K
|
K

A

A

Y

F

A

A

M

M

T

T

G

G

W

W

R

R

I

I

G

G

Y

Y

A

A

G

C

G

G

P

P

R

K

E

E

L

V

I

I

K

K

A

A

M

M

A

A

V

S

V

V

Q

S

S

S

Q

Q

A

S

T

T

S

T

C

S

P

P

S

D

S

T

I

I

S

A

Q

Q

A

W

A

A

S

T

V

L

A

E

A

A

L

L

N

T

G

N

P

Q

Q

E

-

A

-

S

-

R

D

A

F

F

L

V

K

E

E

M

R

A

T

R

E

E

S

A

F

Y

Q

R

R

R

R

R

R

R

D

D

L

L

V

L

V

L

N

E

G

G

L

L

N

T

A

A

I

L

D

-

G

G

L

L

D

K

C

A

R

V

V

R

P

P

E

S

G

G

A

A

F

F

Y

Y

T

-

F

-

S

-

G

-

C

-

A

-

G

-

V

V

L

L

G

M

K

D

V

T

T

S

P

P

S

-

G

-

K

-

R

-

I

I

K

A

T

P

D

D

T

E

D

V

F

R

C

A

A

A

Y

E

L

R

L

L

E

L

D

E

A

A

H

G

V

V

A

A

V

V

V

V

P

P

G

G

S

T

A

D

F

F

G

A

L

A

S

F

P

G

F

H

F

V

R
|
R

I

L

S

S

Y

Y

A

A

T

T

S

S

E

E

A

E

E

N

L

L

K

R

E

K

A

A

L

L

E

E

R

R

I

F

A

A

active site: W125 (= W135), D203 (= D214), I205 (≠ M216), K234 (= K249)
binding phosphate ion: G39 (= G49), W125 (= W135), N175 (= N185), R361 (= R385)

1b5oA Thermus thermophilus aspartate aminotransferase single mutant 1 (see paper)
45% identity, 94% coverage: 17:403/410 of query aligns to 7:379/382 of 1b5oA

query
sites
1b5oA

R

R

I

V

S

Q

S

A

I

M

G

K

V

P

S

S

E

A

I

T

L

V

K

A

I

V

G

N

A

A

R

K

A

A

A

L

A

E

M

L

K

R

R

R

E

Q

G

G

K

V

P

D

V

L

I

V

I

A

L

L

G

T

A

A

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

E

E

H

H

V

V

K

K

Q

E

A

A

A

A

S

R

D

R

A

A

I

L

H

A

R

Q

G

G

E

K

T

T

K

K

Y

Y

T

A

A

P

L

P

D

A

G

G

T

I

P

P

E

E

L

L

K

R

K

E

A

A

I

L

R

A

E

E

K

K

F

F

Q

R

R

R

E

E

N

N

G

G

L

L

A

S

Y

V

E

T

L

P

D

E

E

E

I

T

T

I

V

V

A

T

T

V

G
|
G

A
x
G

K
x
S

Q

Q

I

A

L

L

F

F

N

N

A

L

M

F

M

Q

A

A

S

I

L

L

D

D

P

P

G

G

D

D

E

E

V

V

I

I

I

V

P

L

T

S

P

P

Y

Y

W
|
W

T

V

S

S

Y

Y

S

P

D

E

I

M

V

V

H

R

I

F

C

A

E

G

G

G

K

V

P

V

V

V

L

E

I

V

A

E

C

T

D

L

A

P

S

E

S

E

G

G

F

F

R

V

L

P

T

D

A

P

E

E

K

R

L

V

E

R

A

R

A

A

I

I

T

T

P

P

R

R

T

T

R

K

W

A

V

L

L

V

L

V

N

N

S

S

P

P

S

N

N
|
N

P

P

S

T

G

G

A

A

A

V

Y

Y

S

P

A

K

A

E

D

V

Y

L

R

E

P

A

L

L

L

A

E

R

V

L

L

A

L

V

R

E

H

H

P

-

H

D

V

F

W

Y

L

L

L

V

V

S

D
|
D

D

E

M
x
I

Y
|
Y

E

E

H

H

I

L

V

L

Y

Y

D

E

G

G

F

E

R

H

F

F

V

-

T

S

P

P

A

G

Q

R

L

V

E

A

P

P

G

-

L

-

K

-

N

E

R

H

T

T

L

L

T

T

V

V

N

N

G

G

V

A

S
x
A

K
|
K

A

A

Y

F

A

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

A

A

G

C

G

G

P

P

R

K

E

E

L

V

I

I

K

K

A

A

M

M

A

A

V

S

V

V

Q

S

S

S

Q

Q

A

S

T

T

S

T

C

S

P

P

S

D

S

T

I

I

S

A

Q

Q

A

W

A

A

S

T

V

L

A

E

A

A

L

L

N

T

G

N

P

Q

Q

E

-

A

-

S

-

R

D

A

F

F

L

V

K

E

E

M

R

A

T

R

E

E

S

A

F

Y

Q

R

R

R

R

R

R

R

D

D

L

L

V

L

V

L

N

E

G

G

L

L

N

T

A

A

I

L

D

-

G

G

L

L

D

K

C

A

R

V

V

R

P

P

E

S

G

G

A

A

F

F

Y

Y

T

-

F

-

S

-

G

-

C

-

A

-

G

-

V

V

L

L

G

M

K

D

V

T

T

S

P

P

S

-

G

-

K

-

R

-

I

I

K

A

T

P

D

D

T

E

D

V

F

R

C

A

A

A

Y

E

L

R

L

L

E

L

D

E

A

A

H

G

V

V

A

A

V

V

V

V

P

P

G

G

S

T

A

D

F

F

G

A

L

A

S

F

P

G

F

H

F

V

R

R

I

L

S

S

Y

Y

A

A

T

T

S

S

E

E

A

E

E

N

L

L

K

R

E

K

A

A

L

L

E

E

R

R

I

F

A

A

active site: W125 (= W135), D203 (= D214), I205 (≠ M216), K234 (= K249)
binding pyridoxal-5'-phosphate: G99 (= G109), G100 (≠ A110), S101 (≠ K111), W125 (= W135), N175 (= N185), D203 (= D214), I205 (≠ M216), Y206 (= Y217), A233 (≠ S248), K234 (= K249), R242 (= R257)

1gc4A Thermus thermophilus aspartate aminotransferase tetra mutant 2 complexed with aspartate (see paper)
44% identity, 94% coverage: 17:403/410 of query aligns to 7:379/382 of 1gc4A

query
sites
1gc4A

R

R

I

V

S

Q

S

A

I

M

G

K

V

P

S

D

E

A

I

V

L

V

K

A

I

V

G

N

A

A

R

K

A

A

A

L

A

E

M

L

K

R

R

R

E

Q

G

G

K

V

P

D

V

L

I

V

I

A

L

L

G

T

A

A

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

E

E

H

H

V

V

K

K

Q

E

A

A

A

A

S

R

D

R

A

A

I

L

H

A

R

Q

G

G

E

K

T

T

K

K

Y
|
Y

T

A

A

P

L

P

D

A

G

G

T

I

P

P

E

E

L

L

K

R

K

E

A

A

I

L

R

A

E

E

K

K

F

F

Q

R

R

R

E

E

N

N

G

G

L

L

A

S

Y

V

E

T

L

P

D

E

E

E

I

T

T

I

V

V

A

T

T

V

G
|
G

A
x
G

K
x
S

Q

Q

I

A

L

L

F

F

N

N

A

L

M

F

M

Q

A

A

S

I

L

L

D

D

P

P

G

G

D

D

E

E

V

V

I

I

I

V

P

L

T

S

P

P

Y

Y

W
|
W

T

V

S

S

Y

Y

S

P

D

E

I

M

V

V

H

R

I

F

C

A

E

G

G

G

K

V

P

V

V

V

L

E

I

V

A

E

C

T

D

L

A

P

S

E

S

E

G

G

F

F

R

V

L

P

T

D

A

P

E

E

K

R

L

V

E

R

A

R

A

A

I

I

T

T

P

P

R

R

T

T

R

K

W

A

V

L

L

V

L

V

N

N

S

S

P

P

S

N

N
|
N

P

P

S

T

G

G

A

A

A

V

Y

Y

S

P

A

K

A

E

D

V

Y

L

R

E

P

A

L

L

L

A

E

R

V

L

L

A

L

V

R

E

H

H

P

-

H

D

V

F

W

Y

L

L

L

V

V

S

D
|
D

D

E

M

I

Y
|
Y

E

E

H

H

I

L

V

L

Y

Y

D

E

G

G

F

E

R

H

F

F

V

-

T

S

P

P

A

G

Q

R

L

V

E

A

P

P

G

-

L

-

K

-

N

E

R

H

T

T

L

L

T

T

V

V

N

N

G

G

V

A

S

A

K

K

A

A

Y

F

A

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

A

A

G

C

G

G

P

P

R

K

E

E

L

V

I

I

K

K

A

A

M

M

A

A

V

S

V

V

Q

S

S
x
R

Q

Q

A

S

T

T

S

T

C

S

P

P

S

D

S

T

I

I

S

A

Q

Q

A

W

A

A

S

T

V

L

A

E

A

A

L

L

N

T

G

N

P

Q

Q

E

-

A

-

S

-

R

D

A

F

F

L

V

K

E

E

M

R

A

T

R

E

E

S

A

F

Y

Q

R

R

R

R

R

R

R

D

D

L

L

V

L

V

L

N

E

G

G

L

L

N

T

A

A

I

L

D

-

G

G

L

L

D

K

C

A

R

V

V

R

P

P

E

S

G

G

A

A

F

F

Y
|
Y

T

-

F

-

S

-

G

-

C

-

A

-

G

-

V

V

L

L

G

M

K

D

V

T

T

S

P

P

S

-

G

-

K

-

R

-

I

I

K

A

T

P

D

D

T

E

D

V

F

R

C

A

A

A

Y

E

L

R

L

L

E

L

D

E

A

A

H

G

V

V

A

A

V

V

V

V

P

P

G

G

S

T

A

D

F

F

G

A

L

A

S

F

P

G

F

H

F

V

R
|
R

I

L

S

S

Y

Y

A

A

T

T

S

S

E

E

A

E

E

N

L

L

K

R

E

K

A

A

L

L

E

E

R

R

I

F

A

A

binding aspartic acid: Y64 (= Y74), W125 (= W135), N175 (= N185), R261 (≠ S276), Y322 (= Y335), R361 (= R385)
binding pyridoxal-5'-phosphate: G99 (= G109), G100 (≠ A110), S101 (≠ K111), W125 (= W135), N175 (= N185), D203 (= D214), Y206 (= Y217), R242 (= R257)

1gc3A Thermus thermophilus aspartate aminotransferase tetra mutant 2 complexed with tryptophan (see paper)
44% identity, 94% coverage: 17:403/410 of query aligns to 7:379/382 of 1gc3A

query
sites
1gc3A

R

R

I

V

S

Q

S

A

I

M

G

K

V

P

S

D

E

A

I
x
V

L

V

K

A

I

V

G
x
N

A

A

R

K

A

A

A

L

A

E

M

L

K

R

R

R

E

Q

G

G

K

V

P

D

V

L

I

V

I

A

L

L

G
x
T

A
|
A

G
|
G

E
|
E

P

P

D

D

F

F

D

D

T

T

P

P

E

E

H

H

V

V

K

K

Q

E

A

A

A

A

S

R

D

R

A

A

I

L

H

A

R

Q

G

G

E

K

T

T

K

K

Y

Y

T

A

A

P

L

P

D

A

G

G

T

I

P

P

E

E

L

L

K

R

K

E

A

A

I

L

R

A

E

E

K

K

F

F

Q

R

R

R

E

E

N

N

G

G

L

L

A

S

Y

V

E

T

L

P

D

E

E

E

I

T

T

I

V

V

A

T

T

V

G

G

A
x
G

K
x
S

Q

Q

I

A

L

L

F

F

N

N

A

L

M

F

M

Q

A

A

S

I

L

L

D

D

P

P

G

G

D

D

E

E

V

V

I

I

I

V

P

L

T

S

P

P

Y

Y

W
|
W

T

V

S

S

Y

Y

S

P

D

E

I

M

V

V

H

R

I

F

C

A

E

G

G

G

K

V

P

V

V

V

L

E

I

V

A

E

C

T

D

L

A

P

S

E

S

E

G

G

F

F

R

V

L

P

T

D

A

P

E

E

K

R

L

V

E

R

A

R

A

A

I

I

T

T

P

P

R

R

T

T

R

K

W

A

V

L

L

V

L

V

N

N

S

S

P

P

S

N

N
|
N

P

P

S

T

G

G

A

A

A

V

Y

Y

S

P

A

K

A

E

D

V

Y

L

R

E

P

A

L

L

L

A

E

R

V

L

L

A

L

V

R

E

H

H

P

-

H

D

V

F

W

Y

L

L

L

V

V

S

D
|
D

D

E

M

I

Y
|
Y

E

E

H

H

I

L

V

L

Y

Y

D

E

G

G

F

E

R

H

F

F

V

-

T

S

P

P

A

G

Q

R

L

V

E

A

P

P

G

-

L

-

K

-

N

E

R

H

T

T

L

L

T

T

V

V

N

N

G

G

V

A

S

A

K
|
K

A

A

Y

F

A

A

M

M

T

T

G

G

W

W

R
|
R

I

I

G

G

Y

Y

A

A

G

C

G

G

P

P

R

K

E

E

L

V

I

I

K

K

A

A

M

M

A

A

V

S

V

V

Q

S

S

R

Q

Q

A

S

T

T

S

T

C

S

P

P

S

D

S

T

I

I

S

A

Q

Q

A

W

A

A

S

T

V

L

A

E

A

A

L

L

N

T

G

N

P

Q

Q

E

-

A

-

S

-

R

D

A

F

F

L

V

K

E