SitesBLAST
Comparing SMc04390 FitnessBrowser__Smeli:SMc04390 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
39% identity, 95% coverage: 3:525/551 of query aligns to 5:451/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G9), G13 (= G11), S14 (≠ P12), A15 (= A13), E35 (= E33), A36 (= A34), W47 (= W59), P65 (≠ T77), G67 (≠ A79), V180 (= V217), A214 (≠ S251), G215 (= G252), A218 (≠ G255), T270 (≠ A319), Y391 (≠ H465), A424 (≠ S498), I435 (≠ T509), N436 (= N510)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
34% identity, 95% coverage: 3:526/551 of query aligns to 24:571/578 of 5nccA
- active site: R347 (≠ T316), L420 (≠ S379), I421 (≠ A380), S507 (≠ D464), A509 (≠ H466), G552 (≠ C507), Q553 (≠ N508)
- binding flavin-adenine dinucleotide: G30 (= G9), G32 (= G11), T33 (≠ P12), A34 (= A13), L53 (= L32), E54 (= E33), A55 (= A34), F74 (≠ T53), W80 (= W59), A98 (≠ T77), G100 (≠ A79), G105 (= G84), S106 (≠ G85), N110 (= N89), A111 (= A90), T112 (≠ Q91), L113 (= L92), V238 (= V217), A278 (≠ S251), H282 (≠ G255), L286 (= L259), N508 (≠ H465), Q553 (≠ N508), T554 (= T509), G555 (≠ N510), V558 (≠ T513)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
34% identity, 95% coverage: 3:526/551 of query aligns to 84:638/654 of A0A248QE08
- TA 93:94 (≠ PA 12:13) binding
- E114 (= E33) binding
- L162 (≠ V81) binding
- S166 (≠ G85) binding
- NATL 170:173 (≠ NAQL 89:92) binding
- V298 (= V217) binding
- C432 (≠ F336) binding
- R451 (≠ H356) binding
- Y466 (vs. gap) binding
- Q486 (≠ N378) binding
- G622 (≠ N510) binding
6yrvAAA structure of fap after illumination at 100k (see paper)
34% identity, 95% coverage: 3:526/551 of query aligns to 8:562/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ H356), N499 (≠ H465)
- binding flavin-adenine dinucleotide: G14 (= G9), G16 (= G11), T17 (≠ P12), A18 (= A13), L37 (= L32), E38 (= E33), A39 (= A34), F58 (≠ T53), W64 (= W59), A82 (≠ T77), G89 (= G84), S90 (≠ G85), N94 (= N89), A95 (= A90), T96 (≠ Q91), L97 (= L92), M191 (≠ T186), V222 (= V217), C264 (≠ S250), A265 (≠ S251), G266 (= G252), H269 (≠ G255), N499 (≠ H465), A534 (≠ S498), Q544 (≠ N508), T545 (= T509), G546 (≠ N510)
- binding heptadecane: V377 (≠ G358), G379 (= G360), M380 (≠ S361), G386 (= G366), T389 (vs. gap), Y390 (vs. gap), F393 (vs. gap), T408 (= T376), Q410 (≠ N378)
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
36% identity, 95% coverage: 3:525/551 of query aligns to 14:527/530 of 3ljpA
- active site: I333 (≠ T338), P377 (≠ S379), N378 (≠ A380), A464 (≠ D464), H466 (= H466), V509 (≠ C507), N510 (= N508)
- binding dihydroflavine-adenine dinucleotide: G22 (= G11), S23 (≠ P12), E44 (= E33), A45 (= A34), W71 (= W59), R89 (≠ Q78), A90 (= A79), G95 (= G84), C96 (≠ G85), H99 (≠ I88), N100 (= N89), S101 (≠ A90), I103 (≠ L92), A232 (≠ V217), T269 (≠ S251), D273 (≠ G255), Y465 (≠ H465), H466 (= H466), D499 (= D497), A500 (≠ S498), N510 (= N508), P511 (≠ T509), N512 (= N510), V515 (≠ T513)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
36% identity, 95% coverage: 3:525/551 of query aligns to 14:527/527 of 2jbvA
- active site: I333 (≠ T338), P377 (≠ S379), N378 (≠ A380), V464 (≠ D464), H466 (= H466), V509 (≠ C507), N510 (= N508)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G11), S23 (≠ P12), E44 (= E33), A45 (= A34), W71 (= W59), A90 (= A79), G95 (= G84), C96 (≠ G85), H99 (≠ I88), N100 (= N89), S101 (≠ A90), I103 (≠ L92), R231 (= R216), A232 (≠ V217), T269 (≠ S251), G270 (= G252), D273 (≠ G255), V464 (≠ D464), Y465 (≠ H465), H466 (= H466), D499 (= D497), A500 (≠ S498), N510 (= N508), P511 (≠ T509), N512 (= N510), V515 (≠ T513)
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
36% identity, 95% coverage: 3:525/551 of query aligns to 14:527/532 of 4mjwA
- active site: I333 (≠ T338), P377 (≠ S379), N378 (≠ A380), V464 (≠ D464), H466 (= H466), V509 (≠ C507), N510 (= N508)
- binding flavin-adenine dinucleotide: G20 (= G9), G22 (= G11), S23 (≠ P12), E44 (= E33), A45 (= A34), W71 (= W59), R89 (≠ Q78), A90 (= A79), G95 (= G84), C96 (≠ G85), H99 (≠ I88), N100 (= N89), S101 (≠ A90), I103 (≠ L92), R231 (= R216), A232 (≠ V217), T269 (≠ S251), G270 (= G252), D273 (≠ G255), Y465 (≠ H465), H466 (= H466), A500 (≠ S498), N510 (= N508), P511 (≠ T509), N512 (= N510), V515 (≠ T513)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
31% identity, 95% coverage: 3:526/551 of query aligns to 2:563/565 of 5oc1A
- active site: V339 (≠ A319), N413 (≠ S379), A414 (= A380), I499 (≠ D464), H501 (= H466), A544 (≠ C507), H545 (≠ N508)
- binding 4-methoxybenzoic acid: Y91 (≠ A90), I356 (vs. gap), I390 (≠ Q354), F396 (≠ I363), T412 (≠ N378), I499 (≠ D464), H501 (= H466), H545 (≠ N508)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), N11 (≠ P12), A12 (= A13), E32 (= E33), A33 (= A34), W60 (= W59), P78 (≠ T77), G80 (≠ A79), G85 (= G84), S86 (≠ G85), H90 (≠ N89), Y91 (≠ A90), V93 (≠ L92), V230 (= V217), S270 (= S250), A271 (≠ S251), G272 (= G252), F500 (≠ H465), H545 (≠ N508), T546 (= T509), Q547 (≠ N510), I550 (≠ T513)
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
31% identity, 95% coverage: 3:526/551 of query aligns to 2:563/565 of 3fimB
- active site: V339 (≠ A319), N413 (≠ S379), A414 (= A380), I499 (≠ D464), H501 (= H466), A544 (≠ C507), H545 (≠ N508)
- binding flavin-adenine dinucleotide: G8 (= G9), N11 (≠ P12), A12 (= A13), E32 (= E33), A33 (= A34), W60 (= W59), P78 (≠ T77), G80 (≠ A79), G85 (= G84), S86 (≠ G85), H90 (≠ N89), Y91 (≠ A90), V93 (≠ L92), V230 (= V217), S270 (= S250), A271 (≠ S251), F500 (≠ H465), H501 (= H466), H545 (≠ N508), T546 (= T509), Q547 (≠ N510), I550 (≠ T513)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
33% identity, 94% coverage: 4:521/551 of query aligns to 3:501/509 of 3t37A
- active site: F360 (≠ S379), G361 (≠ A380), H444 (≠ D464), H446 (= H466), G487 (≠ C507), P488 (≠ N508)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), S11 (≠ P12), A12 (= A13), E32 (= E33), A33 (= A34), W58 (= W59), R77 (≠ Q78), G78 (≠ A79), R79 (≠ K80), G83 (= G84), S84 (≠ G85), H88 (≠ N89), A89 (= A90), G91 (≠ L92), R217 (= R216), V218 (= V217), A251 (≠ S251), E255 (≠ G255), H445 (= H465), A478 (≠ S498), P488 (≠ N508), I489 (≠ T509), H490 (≠ N510)
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
33% identity, 94% coverage: 4:521/551 of query aligns to 3:501/508 of 4ha6A
- active site: F360 (≠ S379), G361 (≠ A380), H444 (≠ D464), H446 (= H466), G487 (≠ C507), P488 (≠ N508)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), S11 (≠ P12), A12 (= A13), E32 (= E33), A33 (= A34), W58 (= W59), R77 (≠ Q78), G78 (≠ A79), G83 (= G84), S84 (≠ G85), L87 (≠ I88), H88 (≠ N89), A89 (= A90), M90 (≠ Q91), G91 (≠ L92), V218 (= V217), A251 (≠ S251), G252 (= G252), E255 (≠ G255), H445 (= H465), A478 (≠ S498), P488 (≠ N508), I489 (≠ T509), H490 (≠ N510)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (= A90), S314 (≠ T338), H444 (≠ D464), H446 (= H466)
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
35% identity, 95% coverage: 3:524/551 of query aligns to 6:527/531 of E4QP00
- V101 (≠ I88) mutation to H: Abolishes activity.
- M103 (≠ A90) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (≠ G374) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ T376) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (≠ D464) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ H465) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H466) mutation to A: Abolishes activity.
- N511 (= N508) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
35% identity, 95% coverage: 3:524/551 of query aligns to 2:523/525 of 4udqA
- active site: L331 (= L325), F364 (≠ V375), W365 (≠ T376), V461 (≠ D464), H463 (= H466), A506 (≠ C507), N507 (= N508)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), T11 (≠ P12), A12 (= A13), E32 (= E33), A33 (= A34), W64 (= W59), G88 (≠ A79), G93 (= G84), G94 (= G85), N98 (= N89), M99 (≠ A90), V101 (≠ L92), V229 (= V217), T261 (≠ S250), A262 (≠ S251), W462 (≠ H465), H463 (= H466), A497 (≠ S498), N507 (= N508), T508 (= T509), N509 (= N510), T512 (= T513)
8bxlB Patulin synthase from penicillium expansum
30% identity, 95% coverage: 2:526/551 of query aligns to 13:588/590 of 8bxlB
- binding flavin-adenine dinucleotide: G20 (= G9), G22 (= G11), T23 (≠ P12), A24 (= A13), E44 (= E33), A45 (= A34), W80 (= W59), G100 (≠ A79), G105 (= G84), S106 (≠ G85), R109 (≠ I88), N110 (= N89), Y111 (≠ A90), A113 (≠ L92), L253 (≠ R216), A254 (≠ V217), A288 (≠ S251), Q292 (≠ G255), F525 (≠ H465), D559 (= D497), A560 (≠ S498), H570 (≠ N508), P571 (≠ T509), Q572 (≠ N510), L575 (≠ T513)
Q3L245 Pyranose dehydrogenase 1; PDH 1; Pyranose:quinone oxidoreductase 1; EC 1.1.99.29 from Leucoagaricus meleagris (Western flat-topped agaric) (Agaricus meleagris) (see 2 papers)
31% identity, 95% coverage: 3:524/551 of query aligns to 41:597/602 of Q3L245
- N100 (≠ G60) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H128 (≠ I88) modified: Tele-8alpha-FAD histidine
- N344 (= N288) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H537 (= H466) active site, Proton acceptor
- H581 (≠ N508) active site
Sites not aligning to the query:
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
31% identity, 95% coverage: 3:524/551 of query aligns to 16:572/577 of 4h7uA
- active site: A343 (= A311), V426 (vs. gap), Y510 (≠ D464), H512 (= H466), A555 (≠ C507), H556 (≠ N508)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G9), G24 (= G11), T25 (≠ P12), A26 (= A13), E46 (= E33), A47 (= A34), W74 (= W59), G99 (= G84), C100 (≠ G85), H103 (≠ I88), N104 (= N89), G105 (≠ A90), V107 (≠ L92), L242 (≠ R216), V243 (= V217), G282 (≠ S251), G283 (= G252), A286 (≠ G255), H512 (= H466), A546 (≠ S498), H556 (≠ N508), T557 (= T509), Q558 (≠ N510), V561 (≠ T513)
6h3oF Alcohol oxidase from phanerochaete chrysosporium mutant f101s (see paper)
28% identity, 95% coverage: 4:524/551 of query aligns to 7:619/650 of 6h3oF
- binding flavin-adenine dinucleotide: P15 (= P12), E37 (= E33), G38 (≠ A34), N90 (≠ K80), I91 (≠ V81), G94 (= G84), G95 (= G85), N99 (= N89), S100 (≠ A90), Q101 (= Q91), M102 (≠ L92), R227 (= R216), V228 (= V217), S271 (= S251), I279 (≠ L259), W559 (≠ H465), L593 (≠ S498), N603 (= N508), T604 (= T509), Y605 (≠ N510), A608 (≠ T513)
6h3gB Alcohol oxidase from phanerochaete chrysosporium (see paper)
28% identity, 95% coverage: 4:524/551 of query aligns to 5:617/642 of 6h3gB
- binding flavin-adenine dinucleotide: P13 (= P12), E35 (= E33), G36 (≠ A34), A87 (= A79), G92 (= G84), G93 (= G85), N97 (= N89), F98 (≠ A90), M100 (≠ L92), R225 (= R216), V226 (= V217), S269 (= S251), I277 (≠ L259), W557 (≠ H465), L591 (≠ S498), N601 (= N508), T602 (= T509), Y603 (≠ N510), A606 (≠ T513)
- binding glycerol: R54 (≠ K51), Q57 (≠ K54), N59 (vs. gap)
4yntA Crystal structure of aspergillus flavus fad glucose dehydrogenase (see paper)
30% identity, 95% coverage: 2:525/551 of query aligns to 4:564/570 of 4yntA
- active site: V342 (≠ C301), F413 (≠ Y381), W414 (vs. gap), N502 (≠ D464), H504 (= H466), G546 (≠ C507), H547 (≠ N508)
- binding dihydroflavine-adenine dinucleotide: G13 (= G11), T14 (≠ P12), S15 (≠ A13), E35 (= E33), A36 (= A34), F56 (≠ T53), W62 (= W59), R80 (≠ T77), G82 (≠ A79), G87 (= G84), T88 (≠ G85), N92 (= N89), G93 (≠ A90), M94 (≠ Q91), A95 (≠ L92), A234 (≠ V217), A274 (≠ S251), R278 (≠ G255), F503 (≠ H465), A537 (≠ S498), H547 (≠ N508), L548 (≠ T509), V549 (≠ N510), L552 (≠ T513)
7vzsA Fad-dpendent glucose dehydrogenase complexed with an inhibitor at ph7.56
30% identity, 95% coverage: 2:525/551 of query aligns to 3:563/566 of 7vzsA
- binding D-glucal: Y6 (= Y5), L22 (= L21), N25 (≠ D24), Y51 (≠ F49), I349 (≠ G309), Q356 (≠ T316), E411 (≠ A380), E444 (≠ P411), W445 (≠ H412), K448 (≠ Q415), R499 (≠ K462), N501 (≠ D464), H546 (≠ N508), K563 (≠ R525)
- binding flavin-adenine dinucleotide: G10 (= G9), G12 (= G11), T13 (≠ P12), S14 (≠ A13), E34 (= E33), A35 (= A34), Y51 (≠ F49), F55 (≠ T53), W61 (= W59), R79 (≠ T77), G81 (≠ A79), G86 (= G84), T87 (≠ G85), N91 (= N89), G92 (≠ A90), M93 (≠ Q91), A94 (≠ L92), T232 (≠ R216), A233 (≠ V217), A273 (≠ S251), G274 (= G252), R277 (≠ G255), F502 (≠ H465), A536 (≠ S498), H546 (≠ N508), L547 (≠ T509), V548 (≠ N510), L551 (≠ T513)
Sites not aligning to the query:
Query Sequence
>SMc04390 FitnessBrowser__Smeli:SMc04390
MTYDYIITGAGPAGCVLANRLSEDPDVRVLLLEAGGGDWNPLFHMPAGFAKMTKGVASWG
WHTVPQKHMKGRVLRYTQAKVIGGGSSINAQLYTRGNAADYDLWASEEGCAGWDYRSVLP
YFKRAEDNQRFADDYHSYGGPLGVSMPVSALPICDAYIRAGQELGIPYNHDFNGRQQAGV
GFYQLTQRNRRRSSASLAYLSPIKDRPNLTVRTGARVARIMLEGRRAVGVEVVTGRGSEI
IRADREVLVSSGAIGSPKLLLQSGIGPADHLRSVGVEVRHDLPGVGGNLQDHLDLFVISE
CTGDHTYDGVARLHRTLWAGLQYVLFRSGPVASSLFETGGFWYADPNARSPDIQFHLGLG
SGIEAGVARLKNAGVTLNSAYLHPRSRGTVRLSSADPAAAPLIDPNYWEDPHDRQMSLEG
LKIAREIMQQPALKPFVLDERLPGNGIRTDEQLFDYGCANAKTDHHPVGTCKMGTDAAAV
VDLELKVRGIEGLRVCDSSVMPRVPSCNTNGPTIMMGEKGADIIRGLPPLPPAVFQHERN
DARPRARAEVR
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory