SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing SMc04395 FitnessBrowser__Smeli:SMc04395 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

7cagA Mycobacterium smegmatis lpqy-sugabc complex in the catalytic intermediate state (see paper)
26% identity, 94% coverage: 15:289/292 of query aligns to 13:280/285 of 7cagA

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binding alpha-D-glucopyranose: E159 (≠ A164), K162 (≠ Q167), R216 (≠ L221), D219 (≠ L224)

P94529 Arabinooligosaccharides transport system permease protein AraP from Bacillus subtilis (strain 168) (see paper)
28% identity, 87% coverage: 12:266/292 of query aligns to 32:286/313 of P94529

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E205 (= E186) mutation to A: Has no effect on the growth rate.
E208 (≠ K189) mutation to A: Small negative impact in the uptake of arabinotriose. Shows an increase of about 10% in the doubling time.
D213 (= D194) mutation to A: Severely impairs the uptake of arabinotriose. Shows a 3.6-fold increase of the doubling time.

Query Sequence

>SMc04395 FitnessBrowser__Smeli:SMc04395
MDSRNRLQELLPKLVLAPSFLIVLVFVYGFIAYTGFLSMTDSKMLPSYNFVGLSNYSRLW
ALPHWWRAVSNLAIFATLYIAVCSVLGLALAILLDQKIRAEGFLRPVYLYPMALSFIVTG
TAWKWFLDPGIGLENTMHLWGWESFSFNWIKDRNYAIYCVVIAAVWQSTGFIMAMFLAGL
RGVDNEIIKAAQIDGATTPTIYRRIIIPLMRPVFLSAFVVLAHLAIKAYDLIIALTGGGP
GQATELPATFMYSYTFTRNQMAIGASSAIVMLVMIFSIIVPYLYSEVRGGKR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory