SitesBLAST
Comparing SMc04455 FitnessBrowser__Smeli:SMc04455 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1t9cA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
29% identity, 97% coverage: 3:534/548 of query aligns to 9:557/596 of 1t9cA
- active site: Y29 (≠ V23), G31 (= G25), G32 (≠ E26), A33 (≠ S27), I34 (≠ Y28), E55 (= E48), T78 (= T71), F117 (= F110), Q118 (= Q111), E119 (= E112), K167 (≠ E160), R227 (≠ A215), M263 (≠ I251), V290 (≠ P278), V406 (≠ A384), L431 (vs. gap), G432 (= G409), M434 (= M411), D459 (= D436), N486 (= N463), E488 (≠ I465), Q489 (≠ Y466), M491 (≠ T468), V492 (≠ I469), W495 (≠ H472), L517 (= L494), G522 (= G499), L523 (≠ G500), K556 (≠ P533)
- binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (≠ E26), V107 (= V100), P108 (≠ Q101), F117 (= F110), K167 (≠ E160), D288 (≠ E276), R289 (≠ M277), W495 (≠ H472)
- binding flavin-adenine dinucleotide: R157 (= R150), G216 (= G205), A217 (vs. gap), G218 (= G206), N221 (≠ W209), T243 (≠ S231), L244 (≠ F232), Q245 (≠ R233), L261 (≠ V249), M263 (≠ I251), H264 (≠ G252), G283 (= G271), A284 (≠ G272), R285 (= R273), D287 (≠ S275), R289 (≠ M277), V290 (≠ P278), E316 (≠ H298), V317 (≠ P299), N321 (≠ E303), G334 (≠ A316), D335 (vs. gap), A336 (≠ S317), M411 (≠ T389), G429 (≠ A407), G430 (≠ S408)
- binding magnesium ion: D459 (= D436), N486 (= N463), E488 (≠ I465)
1t9dA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
29% identity, 97% coverage: 3:534/548 of query aligns to 9:557/596 of 1t9dA
- active site: Y29 (≠ V23), G31 (= G25), G32 (≠ E26), A33 (≠ S27), I34 (≠ Y28), E55 (= E48), T78 (= T71), F117 (= F110), Q118 (= Q111), E119 (= E112), K167 (≠ E160), R227 (≠ A215), M263 (≠ I251), V290 (≠ P278), V406 (≠ A384), L431 (vs. gap), G432 (= G409), M434 (= M411), D459 (= D436), N486 (= N463), E488 (≠ I465), Q489 (≠ Y466), M491 (≠ T468), V492 (≠ I469), W495 (≠ H472), L517 (= L494), G522 (= G499), L523 (≠ G500), K556 (≠ P533)
- binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (≠ E26), A33 (≠ S27), V107 (= V100), P108 (≠ Q101), F117 (= F110), K167 (≠ E160), M263 (≠ I251), D288 (≠ E276), R289 (≠ M277), W495 (≠ H472)
- binding flavin-adenine dinucleotide: R157 (= R150), G216 (= G205), A217 (vs. gap), G218 (= G206), N221 (≠ W209), T243 (≠ S231), L244 (≠ F232), Q245 (≠ R233), M260 (≠ D248), L261 (≠ V249), H264 (≠ G252), G283 (= G271), A284 (≠ G272), R285 (= R273), D287 (≠ S275), R289 (≠ M277), V290 (≠ P278), E316 (≠ H298), V317 (≠ P299), N321 (≠ E303), G334 (≠ A316), D335 (vs. gap), A336 (≠ S317), Q410 (≠ A388), M411 (≠ T389), G429 (≠ A407), G430 (≠ S408)
- binding magnesium ion: D459 (= D436), N486 (= N463), E488 (≠ I465)
- binding 2,5-dimethyl-pyrimidin-4-ylamine: E55 (= E48), P81 (= P74), Q118 (= Q111), G432 (= G409), M434 (= M411), M464 (= M441)
1t9aA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
29% identity, 97% coverage: 3:534/548 of query aligns to 10:558/597 of 1t9aA
- active site: Y30 (≠ V23), G32 (= G25), G33 (≠ E26), A34 (≠ S27), I35 (≠ Y28), E56 (= E48), T79 (= T71), F118 (= F110), Q119 (= Q111), E120 (= E112), K168 (≠ E160), R228 (≠ A215), M264 (≠ I251), V291 (≠ P278), V407 (≠ A384), L432 (vs. gap), G433 (= G409), M435 (= M411), D460 (= D436), N487 (= N463), E489 (≠ I465), Q490 (≠ Y466), M492 (≠ T468), V493 (≠ I469), W496 (≠ H472), L518 (= L494), G523 (= G499), L524 (≠ G500), K557 (≠ P533)
- binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: G33 (≠ E26), V108 (= V100), P109 (≠ Q101), F118 (= F110), K168 (≠ E160), M264 (≠ I251), D289 (≠ E276), R290 (≠ M277), M492 (≠ T468), V493 (≠ I469), W496 (≠ H472)
- binding flavin-adenine dinucleotide: R158 (= R150), G217 (= G205), A218 (vs. gap), G219 (= G206), N222 (≠ W209), T244 (≠ S231), L245 (≠ F232), Q246 (≠ R233), L262 (≠ V249), M264 (≠ I251), H265 (≠ G252), G284 (= G271), A285 (≠ G272), R286 (= R273), D288 (≠ S275), R290 (≠ M277), V291 (≠ P278), E317 (≠ H298), V318 (≠ P299), N322 (≠ E303), G335 (≠ A316), D336 (vs. gap), A337 (≠ S317), Q411 (≠ A388), M412 (≠ T389), G430 (≠ A407), G431 (≠ S408)
- binding magnesium ion: D460 (= D436), N487 (= N463), E489 (≠ I465)
- binding propyl trihydrogen diphosphate: V407 (≠ A384), G408 (= G385), Q409 (≠ N386), H410 (≠ Y387), M435 (= M411), G459 (= G435), D460 (= D436), A461 (≠ G437), S462 (≠ C438), N487 (= N463), E489 (≠ I465), Q490 (≠ Y466), G491 (= G467), M492 (≠ T468)
- binding 5-{[ethyl(methyl)amino]methyl}-2-methyl-5,6-dihydropyrimidin-4-amine: G433 (= G409), M435 (= M411), M465 (= M441)
1t9dB Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
29% identity, 97% coverage: 3:534/548 of query aligns to 8:543/582 of 1t9dB
- active site: Y28 (≠ V23), G30 (= G25), G31 (≠ E26), A32 (≠ S27), I33 (≠ Y28), E54 (= E48), T77 (= T71), F116 (= F110), Q117 (= Q111), E118 (= E112), K166 (≠ E160), R213 (≠ A215), M249 (≠ I251), V276 (≠ P278), V392 (≠ A384), L417 (vs. gap), G418 (= G409), M420 (= M411), D445 (= D436), N472 (= N463), E474 (≠ I465), Q475 (≠ Y466), M477 (≠ T468), V478 (≠ I469), W481 (≠ H472), L503 (= L494), G508 (= G499), L509 (≠ G500), K542 (≠ P533)
- binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G31 (≠ E26), A32 (≠ S27), V106 (= V100), P107 (≠ Q101), F116 (= F110), K166 (≠ E160), M249 (≠ I251), D274 (≠ E276), R275 (≠ M277), W481 (≠ H472)
- binding flavin-adenine dinucleotide: R156 (= R150), G202 (= G205), A203 (vs. gap), G204 (= G206), N207 (≠ W209), T229 (≠ S231), L230 (≠ F232), Q231 (≠ R233), L247 (≠ V249), M249 (≠ I251), H250 (≠ G252), G269 (= G271), A270 (≠ G272), R271 (= R273), D273 (≠ S275), R275 (≠ M277), V276 (≠ P278), E302 (≠ H298), V303 (≠ P299), N307 (≠ E303), G320 (≠ A316), D321 (vs. gap), A322 (≠ S317), Q396 (≠ A388), M397 (≠ T389), G415 (≠ A407), G416 (≠ S408)
- binding magnesium ion: D445 (= D436), N472 (= N463), E474 (≠ I465)
- binding 2,5-dimethyl-pyrimidin-4-ylamine: E54 (= E48), P80 (= P74), G418 (= G409), M420 (= M411), M450 (= M441)
1t9bA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
29% identity, 97% coverage: 3:534/548 of query aligns to 9:544/583 of 1t9bA
- active site: Y29 (≠ V23), G31 (= G25), G32 (≠ E26), A33 (≠ S27), I34 (≠ Y28), E55 (= E48), T78 (= T71), F117 (= F110), Q118 (= Q111), E119 (= E112), K167 (≠ E160), R214 (≠ A215), M250 (≠ I251), V277 (≠ P278), V393 (≠ A384), L418 (vs. gap), G419 (= G409), M421 (= M411), D446 (= D436), N473 (= N463), E475 (≠ I465), Q476 (≠ Y466), M478 (≠ T468), V479 (≠ I469), W482 (≠ H472), L504 (= L494), G509 (= G499), L510 (≠ G500), K543 (≠ P533)
- binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: V107 (= V100), P108 (≠ Q101), F117 (= F110), D275 (≠ E276), R276 (≠ M277), M478 (≠ T468), W482 (≠ H472)
- binding flavin-adenine dinucleotide: R157 (= R150), G203 (= G205), A204 (vs. gap), G205 (= G206), N208 (≠ W209), T230 (≠ S231), L231 (≠ F232), Q232 (≠ R233), M247 (≠ D248), L248 (≠ V249), M250 (≠ I251), H251 (≠ G252), G270 (= G271), A271 (≠ G272), R272 (= R273), D274 (≠ S275), R276 (≠ M277), V277 (≠ P278), E303 (≠ H298), V304 (≠ P299), N308 (≠ E303), D322 (vs. gap), A323 (≠ S317), Q397 (≠ A388), M398 (≠ T389), G416 (≠ A407), G417 (≠ S408)
- binding magnesium ion: D446 (= D436), N473 (= N463), E475 (≠ I465)
5wkcA Saccharomyces cerevisiae acetohydroxyacid synthase in complex with the herbicide penoxsulam (see paper)
29% identity, 97% coverage: 3:534/548 of query aligns to 9:552/591 of 5wkcA
- active site: Y29 (≠ V23), G31 (= G25), G32 (≠ E26), A33 (≠ S27), I34 (≠ Y28), E55 (= E48), T78 (= T71), F117 (= F110), Q118 (= Q111), E119 (= E112), K167 (≠ E160), R222 (≠ A215), M258 (≠ I251), V285 (≠ P278), V401 (≠ A384), L426 (vs. gap), G427 (= G409), M429 (= M411), D454 (= D436), N481 (= N463), E483 (≠ I465), Q484 (≠ Y466), M486 (≠ T468), V487 (≠ I469), W490 (≠ H472), L512 (= L494), G517 (= G499), L518 (≠ G500), K551 (≠ P533)
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V401 (≠ A384), G402 (= G385), Q403 (≠ N386), H404 (≠ Y387), G427 (= G409), M429 (= M411), G453 (= G435), D454 (= D436), A455 (≠ G437), S456 (≠ C438), M459 (= M441), N481 (= N463), E483 (≠ I465), Q484 (≠ Y466), G485 (= G467), M486 (≠ T468), V487 (≠ I469)
- binding ethaneperoxoic acid: G32 (≠ E26), Q118 (= Q111)
- binding flavin-adenine dinucleotide: R157 (= R150), G211 (= G205), A212 (vs. gap), G213 (= G206), N216 (≠ W209), T238 (≠ S231), L239 (≠ F232), Q240 (≠ R233), L256 (≠ V249), M258 (≠ I251), G278 (= G271), A279 (≠ G272), R280 (= R273), R284 (≠ M277), V285 (≠ P278), E311 (≠ H298), V312 (≠ P299), N316 (≠ E303), D330 (vs. gap), A331 (≠ S317), M406 (≠ T389), G424 (≠ A407)
- binding magnesium ion: D454 (= D436), N481 (= N463), E483 (≠ I465)
- binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: G32 (≠ E26), A33 (≠ S27), V107 (= V100), F117 (= F110), K167 (≠ E160), M258 (≠ I251), R284 (≠ M277), M486 (≠ T468), W490 (≠ H472)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: P30 (= P24), E55 (= E48)
1n0hA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorimuron ethyl (see paper)
28% identity, 97% coverage: 3:534/548 of query aligns to 11:560/599 of 1n0hA
- active site: Y31 (≠ V23), G33 (= G25), G34 (≠ E26), A35 (≠ S27), I36 (≠ Y28), E57 (= E48), T80 (= T71), F119 (= F110), Q120 (= Q111), E121 (= E112), K169 (≠ E160), R230 (≠ A215), M266 (≠ I251), V293 (≠ P278), V409 (≠ A384), L434 (vs. gap), G435 (= G409), M437 (= M411), D462 (= D436), N489 (= N463), E491 (≠ I465), Q492 (≠ Y466), M494 (≠ T468), V495 (≠ I469), W498 (≠ H472), L520 (= L494), G525 (= G499), L526 (≠ G500), K559 (≠ P533)
- binding 4-{[(4'-amino-2'-methylpyrimidin-5'-yl)methyl]amino}pent-3-enyl diphosphate: V409 (≠ A384), G410 (= G385), Q411 (≠ N386), H412 (≠ Y387), G435 (= G409), M437 (= M411), G461 (= G435), D462 (= D436), A463 (≠ G437), S464 (≠ C438), M467 (= M441), N489 (= N463), E491 (≠ I465), Q492 (≠ Y466), G493 (= G467), V495 (≠ I469)
- binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: G34 (≠ E26), A35 (≠ S27), V109 (= V100), P110 (≠ Q101), F119 (= F110), K169 (≠ E160), M266 (≠ I251), D291 (≠ E276), R292 (≠ M277), V495 (≠ I469), W498 (≠ H472)
- binding flavin-adenine dinucleotide: R159 (= R150), G219 (= G205), A220 (vs. gap), G221 (= G206), N224 (≠ W209), T246 (≠ S231), L247 (≠ F232), Q248 (≠ R233), L264 (≠ V249), G265 (= G250), M266 (≠ I251), H267 (≠ G252), G286 (= G271), A287 (≠ G272), R288 (= R273), D290 (≠ S275), R292 (≠ M277), V293 (≠ P278), E319 (≠ H298), V320 (≠ P299), N324 (≠ E303), G337 (≠ A316), D338 (vs. gap), A339 (≠ S317), M414 (≠ T389), G432 (≠ A407), G433 (≠ S408)
- binding magnesium ion: D462 (= D436), N489 (= N463), E491 (≠ I465)
- binding thiamine diphosphate: Y31 (≠ V23), E57 (= E48), P83 (= P74)
4rjiC Acetolactate synthase from bacillus subtilis bound to thdp - crystal form i (see paper)
29% identity, 97% coverage: 4:532/548 of query aligns to 4:533/555 of 4rjiC
- binding magnesium ion: D438 (= D436), D465 (≠ N463), T467 (≠ I465)
- binding thiamine diphosphate: P24 (= P24), E48 (= E48), P74 (= P74), S387 (≠ N386), H388 (≠ Y387), Q411 (vs. gap), G437 (= G435), D438 (= D436), G439 (= G437), G440 (≠ C438), T467 (≠ I465), Y468 (= Y466), D469 (≠ G467), M470 (≠ T468), V471 (≠ I469)
Sites not aligning to the query:
4rjkF Acetolactate synthase from bacillus subtilis bound to lthdp - crystal form ii (see paper)
29% identity, 97% coverage: 4:532/548 of query aligns to 3:532/552 of 4rjkF
- binding magnesium ion: D437 (= D436), D464 (≠ N463), T466 (≠ I465)
- binding pyruvic acid: A25 (≠ E26), K26 (≠ S27)
- binding thiamine diphosphate: P23 (= P24), E47 (= E48), P73 (= P74), G385 (= G385), S386 (≠ N386), H387 (≠ Y387), Q410 (vs. gap), L412 (vs. gap), G436 (= G435), D437 (= D436), G438 (= G437), G439 (≠ C438), T466 (≠ I465), Y467 (= Y466), D468 (≠ G467), M469 (≠ T468), V470 (≠ I469)
Sites not aligning to the query:
4rjkG Acetolactate synthase from bacillus subtilis bound to lthdp - crystal form ii (see paper)
29% identity, 97% coverage: 4:532/548 of query aligns to 3:532/553 of 4rjkG
- binding magnesium ion: D437 (= D436), D464 (≠ N463), T466 (≠ I465)
- binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: E47 (= E48), Q110 (= Q111)
- binding thiamine diphosphate: I384 (≠ A384), G385 (= G385), S386 (≠ N386), H387 (≠ Y387), Q410 (vs. gap), L412 (vs. gap), G436 (= G435), D437 (= D436), G438 (= G437), G439 (≠ C438), T466 (≠ I465), Y467 (= Y466), D468 (≠ G467), M469 (≠ T468), V470 (≠ I469)
Sites not aligning to the query:
1t9bB Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
28% identity, 97% coverage: 3:534/548 of query aligns to 9:556/595 of 1t9bB
- active site: Y29 (≠ V23), G31 (= G25), G32 (≠ E26), A33 (≠ S27), I34 (≠ Y28), E55 (= E48), T78 (= T71), F117 (= F110), Q118 (= Q111), E119 (= E112), K167 (≠ E160), R226 (≠ A215), M262 (≠ I251), V289 (≠ P278), V405 (≠ A384), L430 (vs. gap), G431 (= G409), M433 (= M411), D458 (= D436), N485 (= N463), E487 (≠ I465), Q488 (≠ Y466), M490 (≠ T468), V491 (≠ I469), W494 (≠ H472), L516 (= L494), G521 (= G499), L522 (≠ G500), K555 (≠ P533)
- binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: V107 (= V100), P108 (≠ Q101), D287 (≠ E276), R288 (≠ M277), M490 (≠ T468), W494 (≠ H472)
- binding flavin-adenine dinucleotide: R157 (= R150), G215 (= G205), A216 (vs. gap), G217 (= G206), N220 (≠ W209), T242 (≠ S231), L243 (≠ F232), Q244 (≠ R233), M259 (≠ D248), L260 (≠ V249), M262 (≠ I251), H263 (≠ G252), G282 (= G271), A283 (≠ G272), R284 (= R273), D286 (≠ S275), R288 (≠ M277), V289 (≠ P278), E315 (≠ H298), V316 (≠ P299), N320 (≠ E303), G333 (≠ A316), D334 (vs. gap), A335 (≠ S317), Q409 (≠ A388), M410 (≠ T389), G428 (≠ A407), G429 (≠ S408)
- binding magnesium ion: D458 (= D436), N485 (= N463), E487 (≠ I465)
6lpiB Crystal structure of ahas holo-enzyme (see paper)
30% identity, 97% coverage: 3:534/548 of query aligns to 7:522/539 of 6lpiB
- active site: I27 (≠ V23), G29 (= G25), G30 (≠ E26), S31 (= S27), I32 (≠ Y28), E53 (= E48), C76 (≠ T71), F115 (= F110), Q116 (= Q111), E117 (= E112), K165 (≠ E160), M256 (≠ I251), A283 (≠ S279), V375 (≠ A384), G401 (= G409), M403 (= M411), D428 (= D436), N455 (= N463), A457 (≠ I465), L458 (≠ Y466), L460 (≠ T468), V461 (≠ I469), Q464 (≠ H472)
- binding flavin-adenine dinucleotide: R155 (= R150), G212 (= G205), G213 (= G206), G214 (≠ T207), T236 (≠ S231), L237 (≠ F232), M238 (≠ R233), L254 (≠ V249), M256 (≠ I251), H257 (≠ G252), G276 (= G271), A277 (≠ G272), R278 (= R273), D280 (≠ S275), R282 (≠ M277), A283 (≠ S279), D300 (≠ H298), I301 (≠ P299), D319 (≠ S317), V320 (≠ P318), M380 (≠ T389), G398 (≠ A404)
- binding magnesium ion: D428 (= D436), N455 (= N463)
- binding thiamine diphosphate: E53 (= E48), C76 (≠ T71), P79 (= P74), G376 (= G385), Q377 (≠ N386), H378 (≠ Y387), G401 (= G409), M403 (= M411), G427 (= G435), D428 (= D436), G429 (= G437), S430 (≠ C438), M433 (= M441), N455 (= N463), A457 (≠ I465), L458 (≠ Y466), G459 (= G467), L460 (≠ T468), V461 (≠ I469)
6u9dB Saccharomyces cerevisiae acetohydroxyacid synthase (see paper)
28% identity, 97% coverage: 3:534/548 of query aligns to 13:568/607 of 6u9dB
- active site: Y33 (≠ V23), G35 (= G25), G36 (≠ E26), A37 (≠ S27), I38 (≠ Y28), E59 (= E48), T82 (= T71), F121 (= F110), Q122 (= Q111), E123 (= E112), K171 (≠ E160), M274 (≠ I251), V301 (≠ P278), V417 (≠ A384), G443 (= G409), M445 (= M411), D470 (= D436), N497 (= N463), E499 (≠ I465), Q500 (≠ Y466), M502 (≠ T468), V503 (≠ I469), W506 (≠ H472)
- binding methyl 2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoylmethyl]benzoate: G36 (≠ E26), V111 (= V100), P112 (≠ Q101), F121 (= F110), K171 (≠ E160), D299 (≠ E276), R300 (≠ M277), M502 (≠ T468), W506 (≠ H472)
- binding flavin-adenine dinucleotide: R161 (= R150), A228 (vs. gap), G229 (= G206), N232 (≠ W209), T254 (≠ S231), L255 (≠ F232), Q256 (≠ R233), L272 (≠ V249), M274 (≠ I251), G294 (= G271), R296 (= R273), D298 (≠ S275), R300 (≠ M277), V301 (≠ P278), E327 (≠ H298), V328 (≠ P299), N332 (≠ E303), D346 (vs. gap), A347 (≠ S317), M422 (≠ T389), G440 (≠ A407), G441 (≠ S408)
- binding magnesium ion: D470 (= D436), N497 (= N463)
- binding thiamine diphosphate: E59 (= E48), P85 (= P74), V417 (≠ A384), G418 (= G385), Q419 (≠ N386), H420 (≠ Y387), G443 (= G409), M445 (= M411), A471 (≠ G437), S472 (≠ C438), N497 (= N463), E499 (≠ I465), Q500 (≠ Y466), G501 (= G467), M502 (≠ T468), V503 (≠ I469)
P07342 Acetolactate synthase catalytic subunit, mitochondrial; Acetohydroxy-acid synthase catalytic subunit; AHAS; ALS; EC 2.2.1.6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
28% identity, 97% coverage: 3:532/548 of query aligns to 93:646/687 of P07342
- R241 (= R150) binding
- 355:376 (vs. 252:273, 27% identical) binding
- 407:426 (vs. 298:316, 15% identical) binding
7egvA Acetolactate synthase from trichoderma harzianum with inhibitor harzianic acid (see paper)
28% identity, 97% coverage: 2:534/548 of query aligns to 7:551/590 of 7egvA
- active site: Y28 (≠ V23), G30 (= G25), G31 (≠ E26), A32 (≠ S27), I33 (≠ Y28), E54 (= E48), T77 (= T71), F116 (= F110), Q117 (= Q111), K166 (≠ E160), E220 (≠ A215), M256 (≠ I251), V283 (≠ P278), V400 (≠ A384), L425 (vs. gap), G426 (= G409), M428 (= M411), Q483 (≠ Y466), M485 (≠ T468), V486 (≠ I469), W489 (≠ H472), L511 (= L494), G516 (= G499), I517 (≠ G500)
- binding flavin-adenine dinucleotide: R156 (= R150), G209 (= G205), Q210 (vs. gap), G211 (= G206), T236 (≠ S231), L237 (≠ F232), H238 (≠ R233), G276 (= G271), S277 (≠ G272), R278 (= R273), D280 (≠ S275), R282 (≠ M277), V283 (≠ P278), E309 (≠ H298), I310 (≠ P299), D328 (= D320), V329 (≠ F321), M405 (≠ T389), G423 (≠ A407), G424 (≠ S408)
- binding (2S)-3-methyl-2-[[(2S,4R)-1-methyl-4-[(2E,4E)-octa-2,4-dienoyl]-3,5-bis(oxidanylidene)pyrrolidin-2-yl]methyl]-2-oxidanyl-butanoic acid: F493 (≠ E476), Y494 (= Y477)
- binding magnesium ion: D453 (= D436), N480 (= N463), E482 (≠ I465)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: P29 (= P24), E54 (= E48), Q117 (= Q111), V400 (≠ A384), G401 (= G385), Q402 (≠ N386), H403 (≠ Y387), G426 (= G409), M428 (= M411), D453 (= D436), A454 (≠ G437), S455 (≠ C438), E482 (≠ I465), Q483 (≠ Y466), G484 (= G467), M485 (≠ T468), V486 (≠ I469)
1jscA Crystal structure of the catalytic subunit of yeast acetohydroxyacid synthase: a target for herbicidal inhibitors (see paper)
28% identity, 97% coverage: 3:534/548 of query aligns to 11:541/541 of 1jscA
- active site: Y31 (≠ V23), G33 (= G25), G34 (≠ E26), A35 (≠ S27), I36 (≠ Y28), E57 (= E48), T80 (= T71), F119 (= F110), Q120 (= Q111), E121 (= E112), K169 (≠ E160), M263 (≠ I251), V290 (≠ P278), V406 (≠ A384), G432 (= G409), M434 (= M411), D459 (= D436), N486 (= N463), E488 (≠ V481), K540 (≠ P533)
- binding dihydrogenphosphate ion: G33 (= G25), G34 (≠ E26), Q120 (= Q111)
- binding flavin-adenine dinucleotide: R159 (= R150), G216 (= G205), A217 (vs. gap), G218 (= G206), N221 (≠ W209), T243 (≠ S231), L244 (≠ F232), L261 (≠ V249), G262 (= G250), H264 (≠ G252), G283 (= G271), A284 (≠ G272), R285 (= R273), D287 (≠ S275), R289 (≠ M277), V290 (≠ P278), E316 (≠ H298), V317 (≠ P299), N321 (≠ E303), G334 (≠ A316), D335 (vs. gap), A336 (≠ S317)
- binding magnesium ion: D459 (= D436), N486 (= N463)
- binding thiamine diphosphate: Y31 (≠ V23), P32 (= P24), E57 (= E48), P83 (= P74), V406 (≠ A384), G407 (= G385), Q408 (≠ N386), H409 (≠ Y387), M434 (= M411), D459 (= D436), A460 (≠ G437), S461 (≠ C438), N486 (= N463)
6bd3A Saccharomyces cerevisiae acetohydroxyacid synthase
28% identity, 97% coverage: 3:534/548 of query aligns to 11:538/538 of 6bd3A
- active site: Y31 (≠ V23), G33 (= G25), G34 (≠ E26), A35 (≠ S27), I36 (≠ Y28), E57 (= E48), T80 (= T71), F119 (= F110), Q120 (= Q111), E121 (= E112), K169 (≠ E160), R225 (≠ A215), M261 (≠ I251), V288 (≠ P278), V404 (≠ A384), L429 (vs. gap), G430 (= G409), M432 (= M411), D457 (= D436), N484 (= N463), L498 (= L494), G503 (= G499), L504 (≠ G500), K537 (≠ P533)
- binding flavin-adenine dinucleotide: R159 (= R150), G214 (= G205), A215 (vs. gap), G216 (= G206), N219 (≠ W209), T241 (≠ S231), L242 (≠ F232), Q243 (≠ R233), L259 (≠ V249), G260 (= G250), H262 (≠ G252), G281 (= G271), A282 (≠ G272), R283 (= R273), D285 (≠ S275), R287 (≠ M277), V288 (≠ P278), E314 (≠ H298), V315 (≠ P299), D333 (vs. gap), A334 (≠ S317)
- binding 2-acetyl-thiamine diphosphate: P32 (= P24), E57 (= E48), P83 (= P74)
- binding magnesium ion: D457 (= D436), N484 (= N463)
- binding oxygen molecule: A35 (≠ S27), T80 (= T71), S81 (≠ R72), Q120 (= Q111)
- binding thiamine diphosphate: V404 (≠ A384), G405 (= G385), Q406 (≠ N386), H407 (≠ Y387), G430 (= G409), M432 (= M411), D457 (= D436), A458 (≠ G437), S459 (≠ C438), M462 (= M441), N484 (= N463)
6denA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide iodomuron ethyl (see paper)
28% identity, 96% coverage: 3:529/548 of query aligns to 15:555/599 of 6denA
- active site: Y35 (≠ V23), G37 (= G25), G38 (≠ E26), A39 (≠ S27), I40 (≠ Y28), E61 (= E48), T84 (= T71), F123 (= F110), Q124 (= Q111), E125 (= E112), K173 (≠ E160), K230 (≠ A215), M266 (≠ I251), V293 (≠ P278), V409 (≠ A384), L434 (vs. gap), G435 (= G409), M437 (= M411), D462 (= D436), N489 (= N463), E491 (≠ I465), Q492 (≠ Y466), M494 (≠ T468), V495 (≠ I469), W498 (≠ H472), L520 (= L494), N525 (≠ G499), V526 (≠ G500)
- binding flavin-adenine dinucleotide: R163 (= R150), G219 (= G205), A220 (vs. gap), G221 (= G206), N224 (≠ W209), T246 (≠ S231), L247 (≠ F232), Q248 (≠ R233), L264 (≠ V249), G286 (= G271), A287 (≠ G272), R288 (= R273), D290 (≠ S275), R292 (≠ M277), V293 (≠ P278), E319 (≠ H298), I320 (≠ P299), N324 (≠ E303), D338 (= D320), V339 (≠ F321), M414 (≠ T389), G432 (≠ A407)
- binding ethyl 2-{[(4-iodo-6-methoxypyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: M266 (≠ I251), D291 (≠ E276), R292 (≠ M277), W498 (≠ H472)
- binding magnesium ion: D462 (= D436), N489 (= N463), E491 (≠ I465)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V409 (≠ A384), G410 (= G385), Q411 (≠ N386), H412 (≠ Y387), G435 (= G409), M437 (= M411), G461 (= G435), D462 (= D436), A463 (≠ G437), S464 (≠ C438), N489 (= N463), E491 (≠ I465), Q492 (≠ Y466), G493 (= G467), M494 (≠ T468), V495 (≠ I469)
6demA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide bensulfuron methyl (see paper)
28% identity, 96% coverage: 3:529/548 of query aligns to 13:553/597 of 6demA
- active site: Y33 (≠ V23), G35 (= G25), G36 (≠ E26), A37 (≠ S27), I38 (≠ Y28), E59 (= E48), T82 (= T71), F121 (= F110), Q122 (= Q111), E123 (= E112), K171 (≠ E160), K228 (≠ A215), M264 (≠ I251), V291 (≠ P278), V407 (≠ A384), L432 (vs. gap), G433 (= G409), M435 (= M411), D460 (= D436), N487 (= N463), E489 (≠ I465), Q490 (≠ Y466), M492 (≠ T468), V493 (≠ I469), W496 (≠ H472), L518 (= L494), N523 (≠ G499), V524 (≠ G500)
- binding methyl 2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoylmethyl]benzoate: M264 (≠ I251), D289 (≠ E276), R290 (≠ M277), M492 (≠ T468), W496 (≠ H472)
- binding flavin-adenine dinucleotide: R161 (= R150), G217 (= G205), A218 (vs. gap), G219 (= G206), N222 (≠ W209), T244 (≠ S231), L245 (≠ F232), Q246 (≠ R233), L262 (≠ V249), G284 (= G271), A285 (≠ G272), R286 (= R273), D288 (≠ S275), R290 (≠ M277), V291 (≠ P278), E317 (≠ H298), I318 (≠ P299), N322 (≠ E303), D336 (= D320), V337 (≠ F321), M412 (≠ T389), G430 (≠ A407)
- binding magnesium ion: D460 (= D436), N487 (= N463), E489 (≠ I465)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (≠ A384), G408 (= G385), Q409 (≠ N386), H410 (≠ Y387), M435 (= M411), G459 (= G435), D460 (= D436), A461 (≠ G437), S462 (≠ C438), M465 (= M441), N487 (= N463), E489 (≠ I465), Q490 (≠ Y466), G491 (= G467), M492 (≠ T468), V493 (≠ I469)
Sites not aligning to the query:
6delA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide chlorimuron ethyl (see paper)
28% identity, 96% coverage: 3:529/548 of query aligns to 13:553/597 of 6delA
- active site: Y33 (≠ V23), G35 (= G25), G36 (≠ E26), A37 (≠ S27), I38 (≠ Y28), E59 (= E48), T82 (= T71), F121 (= F110), Q122 (= Q111), E123 (= E112), K171 (≠ E160), K228 (≠ A215), M264 (≠ I251), V291 (≠ P278), V407 (≠ A384), L432 (vs. gap), G433 (= G409), M435 (= M411), D460 (= D436), N487 (= N463), E489 (≠ I465), Q490 (≠ Y466), M492 (≠ T468), V493 (≠ I469), W496 (≠ H472), L518 (= L494), N523 (≠ G499), V524 (≠ G500)
- binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: D289 (≠ E276), R290 (≠ M277), W496 (≠ H472)
- binding flavin-adenine dinucleotide: R161 (= R150), G217 (= G205), A218 (vs. gap), G219 (= G206), N222 (≠ W209), T244 (≠ S231), L245 (≠ F232), Q246 (≠ R233), L262 (≠ V249), G284 (= G271), A285 (≠ G272), R286 (= R273), D288 (≠ S275), R290 (≠ M277), V291 (≠ P278), E317 (≠ H298), I318 (≠ P299), N322 (≠ E303), D336 (= D320), V337 (≠ F321), M412 (≠ T389), G430 (≠ A407)
- binding (3Z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl](formyl)amino}-3-sulfanylpent-3-en-1-yl trihydrogen diphosphate: V407 (≠ A384), G408 (= G385), Q409 (≠ N386), H410 (≠ Y387), G433 (= G409), M435 (= M411), G459 (= G435), D460 (= D436), A461 (≠ G437), S462 (≠ C438), M465 (= M441), N487 (= N463), E489 (≠ I465), Q490 (≠ Y466), G491 (= G467), M492 (≠ T468), V493 (≠ I469)
- binding magnesium ion: D460 (= D436), N487 (= N463), E489 (≠ I465)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (≠ A384), G408 (= G385), Q409 (≠ N386), H410 (≠ Y387), G433 (= G409), M435 (= M411), G459 (= G435), D460 (= D436), A461 (≠ G437), S462 (≠ C438), M465 (= M441), N487 (= N463), E489 (≠ I465), Q490 (≠ Y466), G491 (= G467), M492 (≠ T468), V493 (≠ I469)
Query Sequence
>SMc04455 FitnessBrowser__Smeli:SMc04455
MKTGGQLVVEALAANGVKRISCVPGESYLAVLDALYDADIEVVVCRQEGGAAMMADAWGR
LTGEPGICMVTRGPGATNASAGLHVARQDSVPMILFIGQVQREAREREAFQEIEYRRAFT
EVAKWVGEIDDPARIPEFVTRAFAVATSGRPGPVVLTLPEDMLTQSTEAPAARAYQPVES
HPGPSQIAKLAELLSTAKRPIAILGGTRWSAESVAGFQSFAERWQLPVGCSFRRQMLFDH
LHPNYAGDVGIGINPSLAGEIREADLVLLVGGRFSEMPSSGYTLIDVPYPRQTLVHVHPD
PGELGRVYRPDLAIAASPRDFVAALSNLTPSAEPHWSARTGEMHAAYLKWSTPPEKGPGD
VQMGPIVNWLEANTGPETIFTNGAGNYATWLHRFHRFRRYGTQAAPASGSMGYGLPAAVA
AKHLHPDREVVCFAGDGCFLMHGQEFATAVRYELPIIVLVVNNGIYGTIRMHQEREYPGR
VSATDLTNPDFAALARAYGGHGETVARTEEFAGAFLRARESGKPAIIEIKLDPEAITPTR
TLSEIRKG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory