SitesBLAST
Comparing Synpcc7942_0031 Synpcc7942_0031 aminotransferase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4cxqA Mycobaterium tuberculosis transaminase bioa complexed with substrate kapa (see paper)
41% identity, 98% coverage: 6:420/424 of query aligns to 13:421/427 of 4cxqA
- active site: Y18 (≠ F11), Y149 (= Y138), E212 (= E206), D246 (= D240), A249 (≠ M243), K275 (= K269), Y399 (= Y398)
- binding 7-keto-8-aminopelargonic acid: W56 (= W46), Y149 (= Y138), G308 (= G303), T310 (≠ S305), R392 (= R391)
- binding pyridoxal-5'-phosphate: G116 (= G106), S117 (= S107), Y149 (= Y138), H150 (= H139), G151 (= G140), E212 (= E206), D246 (= D240), I248 (≠ V242), K275 (= K269), P309 (≠ H304), T310 (≠ S305)
3du4A Crystal structure of 7-keto-8-aminopelargonic acid bound 7,8- diaminopelargonic acid synthase in bacillus subtilis (see paper)
39% identity, 100% coverage: 3:424/424 of query aligns to 9:443/448 of 3du4A
- active site: F17 (= F11), Y146 (= Y138), E217 (= E206), D251 (= D240), A254 (≠ M243), K280 (= K269), A417 (≠ Y398)
- binding 7-keto-8-aminopelargonic acid: L82 (≠ A75), Y146 (= Y138), G315 (= G303), S317 (= S305), R410 (= R391)
- binding pyridoxal-5'-phosphate: S112 (≠ N105), G113 (= G106), A114 (≠ S107), Y146 (= Y138), H147 (= H139), E217 (= E206), D251 (= D240), V253 (= V242), A254 (≠ M243), K280 (= K269), H316 (= H304), S317 (= S305)
P53555 L-Lysine--8-amino-7-oxononanoate transaminase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; Diaminopelargonic acid synthase; L-Lysine--8-amino-7-oxononanoate aminotransferase; EC 2.6.1.105 from Bacillus subtilis (strain 168) (see paper)
39% identity, 100% coverage: 3:424/424 of query aligns to 9:443/448 of P53555
- GA 113:114 (≠ GS 106:107) binding
- Y146 (= Y138) binding
- K280 (= K269) modified: N6-(pyridoxal phosphate)lysine
- G315 (= G303) binding
- HS 316:317 (= HS 304:305) binding
- R410 (= R391) binding
4w1vA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a thiazole inhibitor (see paper)
41% identity, 98% coverage: 6:420/424 of query aligns to 13:419/425 of 4w1vA
- active site: Y18 (≠ F11), Y147 (= Y138), E210 (= E206), D244 (= D240), A247 (≠ M243), K273 (= K269), Y397 (= Y398)
- binding dimethyl (2R)-5-(3-fluorophenyl)-1H-pyrrolo[1,2-c][1,3]thiazole-6,7-dicarboxylate 2-oxide: P17 (= P10), Y18 (≠ F11), W54 (= W46), M81 (≠ I73), G83 (≠ A75), Y147 (= Y138), G306 (= G303), P307 (≠ H304), T308 (≠ S305), F392 (≠ L393)
- binding pyridoxal-5'-phosphate: G114 (= G106), S115 (= S107), Y147 (= Y138), H148 (= H139), E210 (= E206), D244 (= D240), I246 (≠ V242), K273 (= K269)
4cxrA Mycobaterium tuberculosis transaminase bioa complexed with 1-(1,3- benzothiazol-2-yl)methanamine (see paper)
41% identity, 98% coverage: 6:420/424 of query aligns to 13:419/425 of 4cxrA
- active site: Y18 (≠ F11), Y147 (= Y138), E210 (= E206), D244 (= D240), A247 (≠ M243), K273 (= K269), Y397 (= Y398)
- binding 1-(1,3-benzothiazol-2-yl)methanamine: Y18 (≠ F11), W54 (= W46), W55 (= W47), A216 (= A212)
- binding pyridoxal-5'-phosphate: G114 (= G106), S115 (= S107), Y147 (= Y138), H148 (= H139), E210 (= E206), D244 (= D240), I246 (≠ V242), K273 (= K269), P307 (≠ H304), T308 (≠ S305)
P9WQ81 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; 7,8-diaminopelargonic acid synthase; DAPAS; Diaminopelargonic acid synthase; EC 2.6.1.62 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
41% identity, 98% coverage: 6:420/424 of query aligns to 20:429/437 of P9WQ81
- Y25 (≠ F11) mutation to A: Does not show detectable activity at 335 nm with SAM, even up to concentrations of 3 mM, and shows approximately 70% reduced activity with high concentrations of DAPA (0.5 mM).
- W64 (= W46) binding
- Y157 (= Y138) binding
- K283 (= K269) modified: N6-(pyridoxal phosphate)lysine
- G316 (= G303) binding
4wydA Adenosylmethionine-8-amino-7-oxononanoate aminotransferase from mycobacterium tuberculosis complexed with a fragment from dsf screening (see paper)
41% identity, 98% coverage: 6:420/424 of query aligns to 13:422/430 of 4wydA
- active site: Y18 (≠ F11), Y150 (= Y138), E213 (= E206), D247 (= D240), A250 (≠ M243), K276 (= K269), Y400 (= Y398)
- binding N-methyl-1-[4-(1H-pyrazol-1-ylmethyl)phenyl]methanamine: Y18 (≠ F11), W57 (= W46), W58 (= W47), Y150 (= Y138), A219 (= A212), G309 (= G303), T311 (≠ S305), F395 (≠ L393)
- binding pyridoxal-5'-phosphate: G117 (= G106), S118 (= S107), Y150 (= Y138), H151 (= H139), E213 (= E206), D247 (= D240), I249 (≠ V242), K276 (= K269), P310 (≠ H304), T311 (≠ S305)
5kgtA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with an inhibitor optimized from hts lead: 1-[4-[4-(3-chlorophenyl)carbonylpiperidin-1- yl]phenyl]ethanone (see paper)
41% identity, 98% coverage: 6:420/424 of query aligns to 13:422/429 of 5kgtA
- active site: Y18 (≠ F11), Y150 (= Y138), E213 (= E206), D247 (= D240), A250 (≠ M243), K276 (= K269), Y400 (= Y398)
- binding 1-[4-[4-(3-chlorophenyl)carbonylpiperidin-1-yl]phenyl]ethanone: M84 (≠ I73), G86 (≠ A75), G309 (= G303), T311 (≠ S305)
- binding pyridoxal-5'-phosphate: S116 (≠ N105), G117 (= G106), S118 (= S107), Y150 (= Y138), H151 (= H139), G152 (= G140), E213 (= E206), D247 (= D240), I249 (≠ V242), K276 (= K269)
5kgsA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with an inhibitor optimized from hts lead: 5-[4-(1,3-benzodioxol-5-ylcarbonyl)piperazin-1-yl]-2, 3-dihydroinden-1-one (see paper)
41% identity, 98% coverage: 6:420/424 of query aligns to 13:422/429 of 5kgsA
- active site: Y18 (≠ F11), Y150 (= Y138), E213 (= E206), D247 (= D240), A250 (≠ M243), K276 (= K269), Y400 (= Y398)
- binding 5-[4-(1,3-benzodioxol-5-ylcarbonyl)piperazin-1-yl]-2,3-dihydroinden-1-one: P17 (= P10), Y18 (≠ F11), W57 (= W46), M84 (≠ I73), G86 (≠ A75), Y150 (= Y138), D162 (≠ E150), G165 (≠ S152), G166 (≠ L153), P310 (≠ H304), T311 (≠ S305), F395 (≠ L393)
- binding pyridoxal-5'-phosphate: G117 (= G106), S118 (= S107), Y150 (= Y138), H151 (= H139), G152 (= G140), E213 (= E206), D247 (= D240), I249 (≠ V242), K276 (= K269)
4xjpA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with an inhibitor optimized from hts lead (see paper)
41% identity, 98% coverage: 6:420/424 of query aligns to 13:422/429 of 4xjpA
- active site: Y18 (≠ F11), Y150 (= Y138), E213 (= E206), D247 (= D240), A250 (≠ M243), K276 (= K269), Y400 (= Y398)
- binding 1-{4-[4-(1,3-benzodioxol-5-ylcarbonyl)piperazin-1-yl]phenyl}ethanone: P17 (= P10), Y18 (≠ F11), W57 (= W46), M84 (≠ I73), G86 (≠ A75), Y150 (= Y138), G165 (≠ S152), G166 (≠ L153), A219 (= A212), G220 (= G213), G309 (= G303), F395 (≠ L393)
- binding pyridoxal-5'-phosphate: G117 (= G106), S118 (= S107), Y150 (= Y138), H151 (= H139), G152 (= G140), E213 (= E206), D247 (= D240), I249 (≠ V242), K276 (= K269), P310 (≠ H304), T311 (≠ S305)
4xjmA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a hts lead compound
41% identity, 98% coverage: 6:420/424 of query aligns to 13:422/429 of 4xjmA
- active site: Y18 (≠ F11), Y150 (= Y138), E213 (= E206), D247 (= D240), A250 (≠ M243), K276 (= K269), Y400 (= Y398)
- binding 3-{1-[(5-acetylthiophen-2-yl)carbonyl]piperidin-4-yl}-N-(3-methoxyphenyl)propanamide: P17 (= P10), Y18 (≠ F11), W57 (= W46), M84 (≠ I73), G86 (≠ A75), Y150 (= Y138), M158 (= M146), G165 (≠ S152), G166 (≠ L153), M167 (≠ F154), W171 (≠ F158), M307 (≠ Y301), G309 (= G303), T311 (≠ S305)
- binding pyridoxal-5'-phosphate: G117 (= G106), S118 (= S107), Y150 (= Y138), H151 (= H139), G152 (= G140), E213 (= E206), D247 (= D240), I249 (≠ V242), K276 (= K269), P310 (≠ H304), T311 (≠ S305)
4xjlA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a hts lead compound
41% identity, 98% coverage: 6:420/424 of query aligns to 13:422/429 of 4xjlA
- active site: Y18 (≠ F11), Y150 (= Y138), E213 (= E206), D247 (= D240), A250 (≠ M243), K276 (= K269), Y400 (= Y398)
- binding N-(1,2,3-benzothiadiazol-5-yl)-4-phenylpiperazine-1-carboxamide: P17 (= P10), Y18 (≠ F11), W57 (= W46), M84 (≠ I73), G86 (≠ A75), Y150 (= Y138), C161 (≠ G149), G165 (≠ S152), G166 (≠ L153), A219 (= A212)
- binding pyridoxal-5'-phosphate: G117 (= G106), S118 (= S107), Y150 (= Y138), H151 (= H139), G152 (= G140), E213 (= E206), D247 (= D240), I249 (≠ V242), K276 (= K269), P310 (≠ H304), T311 (≠ S305)
4wygA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis complexed with a fragment hit (see paper)
41% identity, 98% coverage: 6:420/424 of query aligns to 13:422/429 of 4wygA
- active site: Y18 (≠ F11), Y150 (= Y138), E213 (= E206), D247 (= D240), A250 (≠ M243), K276 (= K269), Y400 (= Y398)
- binding 1-{4-[(4-chloro-1H-pyrazol-1-yl)methyl]phenyl}methanamine: Y18 (≠ F11), W57 (= W46), W58 (= W47), Y150 (= Y138), A219 (= A212), F395 (≠ L393)
- binding pyridoxal-5'-phosphate: G117 (= G106), S118 (= S107), Y150 (= Y138), H151 (= H139), E213 (= E206), D247 (= D240), I249 (≠ V242), K276 (= K269), P310 (≠ H304), T311 (≠ S305)
4wyeA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis complexed with a dsf fragment hit (see paper)
41% identity, 98% coverage: 6:420/424 of query aligns to 13:422/429 of 4wyeA
- active site: Y18 (≠ F11), Y150 (= Y138), E213 (= E206), D247 (= D240), A250 (≠ M243), K276 (= K269), Y400 (= Y398)
- binding phenyl(piperidin-4-yl)methanone: Y18 (≠ F11), W57 (= W46), W58 (= W47), A219 (= A212), F395 (≠ L393), Y400 (= Y398)
- binding pyridoxal-5'-phosphate: G117 (= G106), S118 (= S107), Y150 (= Y138), H151 (= H139), G152 (= G140), E213 (= E206), D247 (= D240), I249 (≠ V242), K276 (= K269), P310 (≠ H304), T311 (≠ S305)
4w1xA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with 1-(4-(4-(3-chlorobenzoyl) piperazin-1-yl)phenyl)ethanone (see paper)
41% identity, 98% coverage: 6:420/424 of query aligns to 13:422/429 of 4w1xA
- active site: Y18 (≠ F11), Y150 (= Y138), E213 (= E206), D247 (= D240), A250 (≠ M243), K276 (= K269), Y400 (= Y398)
- binding 1-{4-[4-(3-chlorobenzoyl)piperazin-1-yl]phenyl}ethanone: P17 (= P10), Y18 (≠ F11), W57 (= W46), M84 (≠ I73), G86 (≠ A75), Y150 (= Y138), G165 (≠ S152), G166 (≠ L153), A219 (= A212), G309 (= G303), T311 (≠ S305)
- binding pyridoxal-5'-phosphate: G117 (= G106), S118 (= S107), Y150 (= Y138), H151 (= H139), G152 (= G140), E213 (= E206), D247 (= D240), I249 (≠ V242), K276 (= K269), P310 (≠ H304), T311 (≠ S305)
4w1wA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with 7-(diethylamino)-3- (thiophene-2-carbonyl)-2h-chromen-2-one (see paper)
41% identity, 98% coverage: 6:420/424 of query aligns to 13:422/429 of 4w1wA
- active site: Y18 (≠ F11), Y150 (= Y138), E213 (= E206), D247 (= D240), A250 (≠ M243), K276 (= K269), Y400 (= Y398)
- binding 7-(diethylamino)-3-(thiophen-2-ylcarbonyl)-2H-chromen-2-one: P17 (= P10), Y18 (≠ F11), W57 (= W46), M84 (≠ I73), G86 (≠ A75), Y150 (= Y138), G165 (≠ S152), G309 (= G303), P310 (≠ H304), R393 (= R391)
- binding pyridoxal-5'-phosphate: G117 (= G106), S118 (= S107), Y150 (= Y138), H151 (= H139), G152 (= G140), E213 (= E206), D247 (= D240), I249 (≠ V242), K276 (= K269), P310 (≠ H304), T311 (≠ S305)
5te2A Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a mechanism-based inhibitor (see paper)
41% identity, 98% coverage: 6:420/424 of query aligns to 13:422/428 of 5te2A
- active site: Y18 (≠ F11), Y150 (= Y138), E213 (= E206), D247 (= D240), A250 (≠ M243), K276 (= K269), Y400 (= Y398)
- binding [5-hydroxy-4-({[6-(3-hydroxypropyl)-4-oxo-1,4-dihydropyridin-3-yl]amino}methyl)-6-methylpyridin-3-yl]methyl dihydrogen phosphate: Y18 (≠ F11), W57 (= W46), G117 (= G106), S118 (= S107), Y150 (= Y138), H151 (= H139), G152 (= G140), D247 (= D240), I249 (≠ V242), K276 (= K269), G309 (= G303), P310 (≠ H304), T311 (≠ S305)
4xjoA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with an inhibitor optimized from hts lead (see paper)
41% identity, 98% coverage: 6:420/424 of query aligns to 13:422/428 of 4xjoA
- active site: Y18 (≠ F11), Y150 (= Y138), E213 (= E206), D247 (= D240), A250 (≠ M243), K276 (= K269), Y400 (= Y398)
- binding 5-[4-(3-chlorobenzoyl)piperazin-1-yl]-1H-inden-1-one: P17 (= P10), Y18 (≠ F11), W57 (= W46), M84 (≠ I73), G86 (≠ A75), Y150 (= Y138), G165 (≠ S152), G166 (≠ L153), A219 (= A212), P310 (≠ H304), T311 (≠ S305)
- binding pyridoxal-5'-phosphate: G117 (= G106), S118 (= S107), Y150 (= Y138), H151 (= H139), G152 (= G140), E213 (= E206), D247 (= D240), I249 (≠ V242), K276 (= K269), P310 (≠ H304), T311 (≠ S305)
4xewA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a hts lead compound (see paper)
41% identity, 98% coverage: 6:420/424 of query aligns to 13:422/428 of 4xewA
- active site: Y18 (≠ F11), Y150 (= Y138), E213 (= E206), D247 (= D240), A250 (≠ M243), K276 (= K269), Y400 (= Y398)
- binding 6-(2-fluorophenyl)[1,3]dioxolo[4,5-g]quinolin-8(5H)-one: P17 (= P10), Y18 (≠ F11), W57 (= W46), Y150 (= Y138), P310 (≠ H304), T311 (≠ S305), R393 (= R391), F395 (≠ L393)
- binding pyridoxal-5'-phosphate: G117 (= G106), S118 (= S107), Y150 (= Y138), H151 (= H139), G152 (= G140), E213 (= E206), D247 (= D240), I249 (≠ V242), K276 (= K269), P310 (≠ H304), T311 (≠ S305)
4wyfA Crystal structure of 7,8-diaminopelargonic acid synthase (bioa) from mycobacterium tuberculosis, complexed with a dsf fragment hit (see paper)
41% identity, 98% coverage: 6:420/424 of query aligns to 13:422/428 of 4wyfA
- active site: Y18 (≠ F11), Y150 (= Y138), E213 (= E206), D247 (= D240), A250 (≠ M243), K276 (= K269), Y400 (= Y398)
- binding N-(1-oxo-1H-inden-5-yl)acetamide: M84 (≠ I73), G86 (≠ A75), G309 (= G303), P310 (≠ H304), T311 (≠ S305)
- binding pyridoxal-5'-phosphate: G117 (= G106), S118 (= S107), Y150 (= Y138), H151 (= H139), G152 (= G140), E213 (= E206), D247 (= D240), I249 (≠ V242), K276 (= K269), P310 (≠ H304), T311 (≠ S305)
Query Sequence
>Synpcc7942_0031 Synpcc7942_0031 aminotransferase
MPSHPHLWFPFTSVKDAPDPLKVVSGKGARLTLADGRELIDCISSWWVNLHGHAHLRIVE
AIAQQAATLEHVIFAGFSHEPAERLAMELCKILPEKLTRVFFSDNGSTAVEVALKMALQY
WHNLDQPRSRILAFDGAYHGDTFGAMSVGERSLFNAPFEKLLFSVEFLPYPETWWGDETV
EAKEAAAIAAVEQALAAGDVAAVIIEPLVQGAGGMRMARPQFLQQLAARVQAAGSLLIAD
EVMTGFGRTGAWFACQRAGIQPDLICLSKGLTGGFLPLSITVATEVIYDTFCSGNPDHTF
YHGHSYTANPLGCAAAIASLELLLDSEAIVQGLEDAHLPGLELLAQHPKVTRPRLTGGIA
ACDLVSDRGGYLDPIGLRVRQAAIARGLLLRPLGNVLYLLPPYCLTPTELQDIYAAIADL
LDEI
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory