SitesBLAST
Comparing Synpcc7942_0116 Synpcc7942_0116 fructokinase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q8ZKR2 Aminoimidazole riboside kinase; AIRs kinase; EC 2.7.1.223 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
31% identity, 93% coverage: 24:323/324 of query aligns to 16:309/319 of Q8ZKR2
- D16 (= D24) binding
- G31 (= G39) binding
- Y101 (≠ I113) binding
- R162 (= R176) binding
- A180 (≠ L194) binding
- A181 (≠ P195) binding
- A183 (≠ I197) binding
- G213 (≠ Q225) binding
- D246 (= D258) binding
- T248 (= T260) binding
- D252 (= D264) binding
- A287 (≠ T301) binding
- A290 (≠ N304) binding
- G292 (= G306) binding
5eynA Crystal structure of fructokinase from vibrio cholerae o395 in fructose, adp, beryllium trifluoride and calcium ion bound form
31% identity, 92% coverage: 24:321/324 of query aligns to 13:305/306 of 5eynA
- active site: G246 (= G261), A247 (= A262), G248 (= G263), D249 (= D264)
- binding adenosine-5'-diphosphate: H91 (≠ Q102), T217 (= T232), G219 (= G234), A220 (≠ D235), G222 (= G237), A238 (≠ P253), V239 (= V254), P241 (≠ A256), T244 (= T259), G246 (= G261), A247 (= A262), G248 (= G263), F251 (= F266), N279 (≠ S295), G282 (= G298), A283 (= A299)
- binding beryllium trifluoride ion: N157 (= N174), R159 (= R176), T245 (= T260), G246 (= G261), A247 (= A262), G248 (= G263), D249 (= D264)
- binding beta-D-fructofuranose: D13 (= D24), G27 (= G38), G28 (= G39), A29 (= A40), N32 (= N43), V84 (= V95), F96 (= F107), F98 (≠ G109), I127 (= I144), N157 (= N174), R159 (= R176), G246 (= G261), D249 (= D264)
Sites not aligning to the query:
5yggA Crystal structure of fructokinase double-mutant (t261c-h108c) from vibrio cholerae o395 in fructose, adp and potassium ion bound form (see paper)
30% identity, 92% coverage: 24:321/324 of query aligns to 17:309/310 of 5yggA
- binding adenosine-5'-diphosphate: K188 (= K201), S190 (≠ A203), T221 (= T232), G223 (= G234), A224 (≠ D235), G226 (= G237), K241 (≠ F252), A242 (≠ P253), V243 (= V254), A251 (= A262), G252 (= G263), F255 (= F266), N283 (≠ S295), G286 (= G298), A287 (= A299)
- binding beta-D-fructofuranose: D17 (= D24), G31 (= G38), G32 (= G39), A33 (= A40), N36 (= N43), V88 (= V95), F100 (= F107), F102 (≠ G109), I131 (= I144), N161 (= N174), R163 (= R176), D253 (= D264)
Sites not aligning to the query:
3lkiB Crystal structure of fructokinase with bound atp from xylella fastidiosa
30% identity, 98% coverage: 7:323/324 of query aligns to 4:319/322 of 3lkiB
- active site: A250 (≠ G261), A251 (= A262), G252 (= G263), D253 (= D264)
- binding adenosine-5'-triphosphate: R161 (= R176), K186 (= K201), T221 (= T232), D222 (= D233), A223 (≠ G234), A224 (≠ D235), V227 (≠ C238), T240 (≠ S251), F241 (= F252), V243 (= V254), V245 (≠ A256), A250 (≠ G261), A251 (= A262), G252 (= G263), F255 (= F266), A291 (≠ S295), G294 (= G298), A295 (= A299)
3iq0B Crystal structure of a putative ribokinase ii in complex with atp and mg+2 from e.Coli
26% identity, 98% coverage: 8:323/324 of query aligns to 4:307/308 of 3iq0B
- active site: G252 (= G261), A253 (= A262), G254 (= G263), D255 (= D264)
- binding adenosine-5'-triphosphate: N164 (= N174), S192 (≠ R204), K223 (≠ T232), R224 (≠ D233), G225 (= G234), N226 (≠ D235), G228 (= G237), Y243 (≠ F252), V245 (= V254), E247 (≠ A256), A253 (= A262), G254 (= G263), F257 (= F266), N279 (≠ S295), G282 (= G298), A283 (= A299), V286 (≠ T302)
1tz3A Crystal structure of aminoimidazole riboside kinase complexed with aminoimidazole riboside (see paper)
30% identity, 93% coverage: 24:323/324 of query aligns to 12:298/299 of 1tz3A
- active site: C24 (≠ W36), F88 (≠ A101), G238 (= G261), A239 (= A262), G240 (= G263), D241 (= D264)
- binding 5-aminoimidazole ribonucleoside: D12 (= D24), G26 (= G38), G27 (= G39), A28 (= A40), L83 (≠ Q96), F88 (≠ A101), Y90 (≠ G103), R151 (= R176), M154 (≠ F179), D241 (= D264)
Sites not aligning to the query:
1tz6A Crystal structure of aminoimidazole riboside kinase from salmonella enterica complexed with aminoimidazole riboside and atp analog (see paper)
30% identity, 92% coverage: 24:322/324 of query aligns to 12:297/297 of 1tz6A
- active site: C24 (≠ W36), F88 (≠ A101), G238 (= G261), A239 (= A262), G240 (= G263), D241 (= D264)
- binding phosphomethylphosphonic acid adenylate ester: D147 (= D172), N149 (= N174), K176 (= K201), S178 (≠ A203), E181 (= E206), S209 (≠ T232), G211 (= G234), A212 (≠ D235), G214 (= G237), A228 (≠ S251), V231 (= V254), V233 (≠ A256), A239 (= A262), G240 (= G263), F243 (= F266), N270 (≠ S295), G273 (= G298), A274 (= A299), V277 (≠ T302)
- binding 5-aminoimidazole ribonucleoside: D12 (= D24), G26 (= G38), G27 (= G39), A28 (= A40), N31 (= N43), L83 (≠ Q96), F88 (≠ A101), Y90 (≠ G103), R151 (= R176), M154 (≠ F179), T237 (= T260), D241 (= D264)
Sites not aligning to the query:
Q53W83 2-dehydro-3-deoxygluconokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; EC 2.7.1.45 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
29% identity, 89% coverage: 36:324/324 of query aligns to 31:304/309 of Q53W83
8cqxA Ribokinase from t.Sp mutant a92g
30% identity, 97% coverage: 8:321/324 of query aligns to 2:298/300 of 8cqxA
- binding adenosine-5'-diphosphate: N179 (≠ A203), E180 (≠ R204), T217 (= T232), L218 (≠ D233), G219 (= G234), A220 (≠ D235), G222 (= G237), A235 (≠ S251), F236 (= F252), V238 (= V254), A246 (= A262), G247 (= G263), F250 (= F266), N272 (≠ S295), G275 (= G298), A276 (= A299), T279 (= T302)
- binding magnesium ion: D242 (= D258), T244 (= T260), A278 (≠ T301), G283 (= G306), S287 (≠ A310)
1v1aA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound 2- keto-3-deoxygluconate and adp (see paper)
29% identity, 88% coverage: 36:321/324 of query aligns to 31:301/301 of 1v1aA
- active site: G248 (= G261), A249 (= A262), G250 (= G263), D251 (= D264)
- binding adenosine-5'-diphosphate: K219 (≠ T232), G221 (= G234), A222 (≠ D235), F239 (= F252), V241 (= V254), A243 (= A256), A249 (= A262), G250 (= G263), F253 (= F266), N275 (≠ S295), G278 (= G298), A279 (= A299), A282 (≠ T302)
- binding 2-keto-3-deoxygluconate: G33 (= G38), G34 (= G39), A35 (= A40), N38 (= N43), Y89 (vs. gap), Y103 (≠ R99), R105 (≠ A101), I134 (≠ T143), R167 (= R176), G248 (= G261), D251 (= D264), D287 (≠ A307)
Sites not aligning to the query:
1v1bA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound atp (see paper)
29% identity, 88% coverage: 36:320/324 of query aligns to 31:300/300 of 1v1bA
- active site: G248 (= G261), A249 (= A262), G250 (= G263), D251 (= D264)
- binding adenosine-5'-triphosphate: N165 (= N174), S193 (≠ A203), K219 (≠ T232), G221 (= G234), G224 (= G237), A238 (≠ S251), F239 (= F252), V241 (= V254), P246 (≠ T259), V247 (≠ T260), G248 (= G261), A249 (= A262), G250 (= G263), D251 (= D264), F253 (= F266), N275 (≠ S295), G278 (= G298), A279 (= A299), A282 (≠ T302)
3ih0A Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with amp-pnp
27% identity, 82% coverage: 8:272/324 of query aligns to 3:254/304 of 3ih0A
- active site: G243 (= G261), A244 (= A262), G245 (= G263), D246 (= D264)
- binding phosphoaminophosphonic acid-adenylate ester: K189 (≠ R204), T214 (= T232), G216 (= G234), P217 (≠ D235), G219 (= G237), F220 (≠ C238), S233 (= S251), V236 (= V254), P238 (≠ A256), T241 (= T259), A244 (= A262), G245 (= G263)
Sites not aligning to the query:
3gbuA Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with atp
27% identity, 82% coverage: 8:272/324 of query aligns to 2:253/302 of 3gbuA
- active site: G242 (= G261), A243 (= A262), G244 (= G263), D245 (= D264)
- binding adenosine-5'-triphosphate: K188 (≠ R204), T213 (= T232), G215 (= G234), P216 (≠ D235), G218 (= G237), F219 (≠ C238), S232 (= S251), V235 (= V254), P237 (≠ A256), T240 (= T259), A243 (= A262), G244 (= G263)
Sites not aligning to the query:
6znxC Ribokinase from thermus species
31% identity, 90% coverage: 31:321/324 of query aligns to 16:263/265 of 6znxC