SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Synpcc7942_0158 FitnessBrowser__SynE:Synpcc7942_0158 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
42% identity, 98% coverage: 10:391/391 of query aligns to 63:453/453 of Q9VLJ8

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Sites not aligning to the query:

62 modified: Phosphothreonine

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
43% identity, 97% coverage: 11:391/391 of query aligns to 55:460/460 of O95396

query
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158:238 (vs. 114:194, 53% identical) Interaction with NFS1
C239 (= C195) mutation to A: Impairs sulfurtransferase activity.
C316 (= C265) modified: Disulfide link with 324; mutation to A: Does not affect sulfurtransferase activity.
C324 (≠ A273) modified: Disulfide link with 316; mutation to A: Does not affect sulfurtransferase activity.
C365 (≠ A314) mutation to A: Does not affect sulfurtransferase activity.
C412 (= C350) active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; mutation to A: Abolishes sulfurtransferase activity.
K413 (= K351) mutation to R: Does not affect sulfurtransferase specificity and activity.
L414 (≠ M352) mutation to K: Does not affect sulfurtransferase specificity and activity.
G415 (= G353) mutation to A: Does not affect sulfurtransferase specificity and activity.
N416 (≠ G354) mutation to V: Does not affect sulfurtransferase specificity and activity.
D417 (≠ R355) mutation to R: Results in 470-fold increased activity.; mutation to T: Results in 90-fold increased activity.
P458 (= P389) mutation to G: Does not affect sulfurtransferase specificity and activity.
Y460 (= Y391) mutation to A: Does not affect sulfurtransferase specificity and activity.

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
53% identity, 66% coverage: 9:267/391 of query aligns to 1:270/271 of Q72J02

query
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C192 (= C195) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.
C268 (= C265) mutation to S: Still able to form a thioester complex with TtuB.

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
38% identity, 97% coverage: 11:391/391 of query aligns to 39:440/440 of P38820

query
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P38820

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N

A

A

L

F

S

D

I

I

L

F

E

K

P

G

Y

Y

D

N

V

Y

I

I

V

L

D

D

G

C

T

T

D

D

N

S

F

P

P

L

T

T

R

R

Y

Y

L

L

V

V

N

S

D

D

A

V

C

A

V

V

L

N

L

L

G

G

K

I

P

T

N

V

V

V

Y

S

G

A

S

S

I

G

F

L

R

G

F

T

E

E

G

G

Q

Q

A

L

T

T

V

I

F

L

N

N

Y

F

E

N

D

N

-

I

G

G

P

P

N

C

Y

Y

R

R

D

C

L

F

Y

Y

P

P

E

T

P

P

G

N

M

A

V

V

P

T

S

S

C
|
C

A

Q

E

E

G

G

V

V

L

I

G

G

I

P

L

C

P

I

G

G

I

L

I

V

G

G

V

T

I

M

Q

M

A

A

T

V

E

E

T

T

V

L

K

K

I

L

I

I

L

L

G

G

-

I

-

Y

Q

T

G

N

Q

E

T

N

L

F

S

S

G

P

R

F

L

L

L

M

L

L

Y

Y

N

S

A

G

L

F

-

P

D

Q

M

Q

K

S

F

L

R

R

E

T

L

F

K

K

L

M

R

R

P

G

N

R

P

Q

E

E

R

K

P

C

V

L

I

C

-

C

-

G

-

K

D

N

R

R

L

T

-

I

-

T

-

K

-

E

-

A

-

I

-

E

-

K

-

G

-

E

I

I

D

N

Y

Y

Q

E

E

L

F

F

C

C

G

G

I

A

P

R

Q

N

A

Y

Q

N

A

V

A

C

E

E

A

P

A

D

A

E

T

-

D

-

S

-

I

-

P

-

E

R

I

I

S

S

V

V

T

D

E

A

L

F

K

Q

A

R

I

I

L

Y

D

-

R

-

Q

K

D

D

P

D

E

E

V

F

L

L

-

A

-

K

-

H

-

I

L

F

I

L

D

D

V

V

R

R

N

P

P

S

H

H

E

H

A

Y

E

E

I

I

A

S

T

H

I

F

P

P

G

E

A

A

I

V

L

N

V

I

P

P

L

I

P

K

D

N

I

L

E

R

A

D

G

M

A

N

G

G

-

D

V

L

E

K

T

K

V

L

R

Q

E

E

L

K

L

L

-

P

-

S

-

V

N

E

G

K

K

D

R

S

E

N

L

I

I

V

V

I

H

L

C
|
C

K

R

M

Y

G

G

G

N

R

D

S

S

A

Q

K

L

A

A

L

T

G

R

I

L

L

L

K

K

E

D

A

K

-

F

G

G

I

F

T

S

G

N

T

V

-

R

N

D

V

V

A

R

G

G

I

Y

N

F

A

K

W

Y

S

I

Q

D

D

D

V

I

D

D

P

Q

S

T

V

I

P

P

Q

K

Y

Y

C225 (= C195) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.
C397 (= C350) mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
49% identity, 68% coverage: 2:267/391 of query aligns to 1:267/270 of D4GSF3

query
sites
D4GSF3

L

M

N

T

L

L

D

S

T

L

D

D

S

A

I

T

Q

Q

L

L

T

D

Q

-

D

-

Y

-

A

-

R

R

Y

Y

S

S

R

R

H

H

L

I

I

I

L

M

P

D

E

E

V

V

G

G

L

P

E

E

G

G

Q

Q

K

G

R

R

L

L

K

L

A

S

K

R

V

V

L

V

C

V

I

V

G

G

T

A

G

G

L

L

G

G

S

A

P

P

L

A

L

I

L

Q

Y

Y

L

L

A

A

A

V

G

G

I

V

G

G

R

E

I

L

G

V

I

V

V

V

D

D

F

D

D

D

I

V

V

V

D

E

H

R

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

V

I

V

H

H

G

C

T

D

S

D

W

D

V

V

G

G

K

T

P

P

K

K

I

A

E

E

S

S

A

A

K

A

N

A

R

F

I

V

H

R

E

G

I

L

N

N

P

P

Y

D

C

V

Q

S

V

V

D

E

L

P

Y

V

E

E

T

A

A

R

L

V

S

D

S

K

E

S

N

N

A

V

L

H

S

E

I

V

L

V

E

A

P

G

Y

S

D

D

V

V

I

V

V

V

D

D

G

A

T

S

D

D

N

N

F

F

P

P

T

T

R

R

Y

Y

L

L

V

L

N

N

D

D

A

V

C

C

V

R

L

F

L

E

G

G

K

I

P

P

N

L

V

V

Y

H

G

G

S

A

I

I

F

Y

R

K

F

F

E

E

G

G

Q

Q

A

A

T

T

V

T

F

L

N

-

Y

V

E

P

D

D

G

G

P

P

N

C

Y

Y

R

R

D

C

L

L

Y

F

P

P

E

E

P

A

P

P

P

E

P

P

G

G

M

T

V

V

P

P

S

D

C
|
C

A

A

E

T

G

T

G

G

V

V

L

L

G

G

I

V

L

L

P

P

G

G

I

T

I

V

G

G

V

C

I

I

Q

Q

A

A

T

T

E

E

T

A

V

M

K

K

I

L

L

L

G

D

Q

E

G

G

Q

E

T

A

L

L

S

D

G

G

R

R

L

L

L

F

Y

Y

N

D

A

A

L

M

D

D

M

M

K

T

F

F

R

E

E

T

L

V

K

P

L

Y

R

R

P

T

N

N

P

P

E

D

R

C

P

P

V

V

-

C

-

G

-

E

-

G

I

V

D

D

R

S

L

I

-

E

-

D

I

I

D

D

Y

Y

Q

V

E

E

F

S

C

C

G

A

I

I

C188 (= C195) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
36% identity, 97% coverage: 11:391/391 of query aligns to 61:482/482 of O59954

query
sites
O59954

L

L

T

H

Q

G

D

E

Y

Y

A

R

R

R

Y

Y

S

G

R

R

H

Q

L

M

I

I

L

V

P

P

E

Q

V

F

G

G

L

L

E

Q

G
|
G

Q

Q

K

L

R

K

L

L

K

R

A

D

A

A

K

K

V

V

L

L

C

I

I

V

G

G

T

A

G

G

L

L

G
|
G

S

C

P

P

L

A

L

L

Y

Y

L

L

A

A

A

G

A

A

G

G

I

V

G

G

R

T

I

I

G

G

I

L

V

V

D

D

F

G

D

D

I

T

V

V

D

E

H

A

S

S

N

N

L

L

Q

H

R
|
R

Q

Q

V

V

I

L

H

H

G

R

T

S

S

R

W

N

V

V

G

G

K

K

P

L

K

K

I

V

E

D

S

S

A

A

K

I

N

E

R

Y

I

L

H

R

E

E

I

L

N

N

P

P

Y

H

C

P

Q

T

V

Y

D

I

L

A

Y

H

E

Q

T

A

A

H

L

L

S

T

S

P

E

R

N

E

A

A

L

P

S

D

I

I

L

F

E

K

P

D

Y

Y

D

D

V

L

I

I

V

L

D

D

G
x
C

T

T

D

D

N

N

F

P

P

A

T

T

R

R

Y

Y

L

L

V

I

N

S

D

D

A

T

C

A

V

V

L

L

G

G

K

K

P

P

N

L

V

V

Y

S

G

A

S

S

I

A

F

L

R

R

F

T

E
|
E

G

G

Q

Q

A

L

T

M

V

V

F

L

N

N

-

N

-

P

-

Q

-

P

-

G

-

D

Y

K

E

T

D

G

G

G

P

P

N

C

Y

Y

R

R

D

C

L

V

Y

F

P

P

E

K

P

P

P

A

G

N

M

S

V

V

P

T

S

S

C

C

A

A

E

D

G

G

V

I

L

L

G

G

I

P

L

V

P

V

G
|
G

I

T

I

M

G

G

V

V

I

L

Q

Q

A

A

T

S

E

E

T

A

V

I

K

K

I

V

I

L

L

T

G

S

Q

A

G

G

Q

E

T

S

L

V

S

E

G

A

-

T

-

P

R

S

L

L

L

I

Y

F

N

S

A

A

L

Y

D

S

M

S

-

P

K

Q

F

F

R

R

E

T

L

I

K

K

L

L

R

R

P

-

N

-

P

S

E

R

R

R

P

P

-

N

-

C

-

A

-

V

-

C

-

S

-

A

-

E

-

A

-

T

-

V

-

T

-

L

-

E

-

S

V

V

I

R

D

S

R

G

L

S

I

M

D

D

Y

Y

Q

V

E

F

F

F

C

C

G

G

I

T

P

-

Q

-

A

V

Q

D

A

P

A

A

E

D

A

I

A

L

A

S

T

P

D

E

S

E

I

-

P

-

E

R

I

I

S

S

V

P

T

S

E

E

L

Y

K

G

A

N

I

V

L

-

D

D

R

S

Q

A

D

G

P

A

E

Q

V

R

L

H

L

I

I

I

D

D

V

V

R

R

N

E

P

K

H

V

E

Q

A

F

E

D

I

I

A

C

T

S

I

L

P

E

G

N

A

S

I

I

L

N

V

I

P

P

L

M

P

S

D

T

I

I

E

L

A

A

G

S

A

A

G

Y

V

S

E

A

T

P

V

T

R

L

E

D

L

A

L

D

N

E

G

P

K

K

R

R

-

L

-

P

-

S

-

W

-

L

-

P

-

E

-

V

-

A

-

H

-

E

-

S

-

N

-

K

E

P

L

I

I

Y

V

V

H

V

C

C

K

R

M

Q

G

G

G

N

R

D

S

S

A

Q

K

T

A

V

L

V

G

R

I

K

L

L

K

K

E

E

A

L

G

G

I

L

T

D

G

H

-

G

-

E

-

R

-

P

-

V

T

V

N

D

V

I

A

K

G

G

I

F

N

R

A

S

W

W

S

R

Q

E

D

Q

V

V

D

D

P

P

S

D

V

W

P

P

Q

D

Y

Y

G82 (= G32) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G50) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R80) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (≠ G135) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (= E165) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (= G206) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
34% identity, 97% coverage: 11:389/391 of query aligns to 6:421/423 of 6yubA

query
sites
6yubA

L

L

T

S

Q

K

D

E

Y

I

A

E

R

R

Y

Y

S

S

R

R

H

Q

L

M

I

I

L

V

P

P

E

G

V

M

G

G

L

K

E

E

G

G

Q

Q

K

L

R

R

L

L

K

M

A

N

A

A

K

K

V

V

L

L

C

I

I

I

G

G

T

A

G

G

L

L

G

G

S

C

P

P

L

A

L

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

I

V

G

G

R

T

I

I

G

G

I

I

V

V

D

D

F

G

D

D

I

S

V

V

D

E

H

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

I

A

H

H

G

A

T

T

S

K

W

R

V

V

G

G

K

M

P

L

K

K

I

V

E

D

S

S

A

L

K

I

N

T

R

H

I

L

H

I

E

E

I

I

N

N

P

P

Y

L

C

P

Q

V

V

Y

D

V

L

P

Y

Y

E

R

T

F

A

D

L

L

S

T

S

P

E

Q

N

N

A

A

L

A

S

Q

I

I

L

I

E

K

P

P

Y

W

D

D

V

V

I

I

V

L

D

D

G

C

T

T

D

D

N

N

F

P

P

A

T

T

R

R

Y

Y

L

L

V

I

N

S

D

D

A

V

C

C

V

V

L

L

G

G

K

K

P

P

N

L

V

V

Y

S

G

A

S

A

I

S

F

V

R

Q

F

K

E

S

G

G

Q

Q

A

L

T

I

V

V

F

L

-

N

-

C

-

P

-

T

-

P

-

Q

-

G

-

V

N

N

Y

K

E

K

D

A

G

A

P

P

N
x
C

Y

Y

R

R

D
x
C

L

C

Y

F

P

K

E

K

P

P

P

-

G

-

M

-

V

-

P

-

S

-

C

-

A

-

E

-

G

-

G

G

V

I

L

M

G

G

I

P

L

V

P

V

G

G

I

M

G

G

V

V

I

A

Q

Q

A

A

T

G

E

E

T

A

V

I

K

K

I

I

I

L

L

V

G

S

Q

Q

-

L

-

H

-

M

-

P

-

K

-

E

G

G

Q

E

T

E

L

V

S

S

G

P

R

E

-

K

-

N

-

L

-

V

-

Q

-

P

-

T

L

L

L

I

Y

Y

N

T

A

-

L

Y

D

D

M

L

-

N

-

S

-

A

-

I

-

G

-

P

-

Y

K

S

F

F

R

R

E

A

L

L

K

K

L

M

R

G

P

G

N

R

P

K

E

K

R

D

P
x
C

V

F

I

A

D
x
C

R

G

L

E

I

N

D

S

Y

T

Q

L

E

T

F

L

C

D

G

G

I

I

-

K

-

S

-

G

-

N

P

P

Q

N

A

Y

Q

V

A

G

A

N

E

M

A

T

A

Q

A

S

T

T

D

N

S

L

I

A

P

P

E

E

I

D

S

R

V

I

T

T

E

A

L

T

K

A

A

Y

I

N

L

E

D

K

R

R

Q

R

D

N

P

G

E

E

V

L

-

G

-

E

-

H

L

I

L

L

I

L

D

D

V

T

R

R

N

E

P

K

H

E

E

H

A

F

E

S

I

F

A

G

T

S

I

I

P

P

G

G

A

A

I

V

L

N

V

V

P

P

L

F

P

S

D

K

-

F

I

L

E

V

A

K

G

A

A

S

G

S

V

I

E

K

T

R

V

P

R

A

E

E

L

L

-

P

-

M

-

Q

-

P

-

A

N

S

G

D

K

E

R

A

E

P

L

I

I

V

V

V

H

V

C

C

K

R

M

R

G

G

G

Q

R

D

S

S

A

Q

K

E

A

V

L

V

G

E

I

K

L

L

K

K

E

E

A

L

G

G

I

L

T

D

G

N

-

G

-

K

-

R

-

K

-

I

T

M

N

D

V

I

A

V

G

G

I

M

N

K

A

A

W

W

S

R

Q

D

D

E

V

V

D

D

P

P

S

D

V

F

P

P

binding zinc ion: C183 (≠ N178), C186 (≠ D181), C266 (≠ P253), C269 (≠ D256)

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
39% identity, 62% coverage: 10:250/391 of query aligns to 3:242/249 of P12282

query
sites
P12282

Q

E

L

L

T

S

Q

D

D

Q

D

E

Y

M

A

L

R

R

Y

Y

S

N

R
|
R

H

Q

L

I

I

I

L

L

P

R

E

G

V

F

G

D

L

F

E

D

G

G

Q

Q

K

E

R

A

L

L

K

K

A

D

A

S

K

R

V

V

L

L

C

I

I

V

G

G

T

L

G

G

L

L

G

G

S
x
C

P

A

L

A

L

S

L

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

I

V

G

G

R

N

I

L

G

T

I

L

V

L

D

D

F

F

D

D

I

T

V

V

D

S

H

L

S

S

N

N

L

L

Q

Q

R
|
R

Q

Q

V

T

I

L

H

H

G

S

T

D

S

A

W

T

V

V

G

G

K

Q

P

P

K

K

I

V

E

E

S

S

A

A

K

R

N

D

R

A

I

L

H

T

E

R

I

I

N

N

P

P

Y

H

C

I

Q

A

V

I

D

T

L

P

Y

V

E

N

T

A

A

L

L

L

S

D

E

A

N

E

A

L

L

A

S

A

I

L

L

I

E

A

P

E

Y

H

D

D

V

L

I

V

V

L

D

D

G
x
C

T

T

D
|
D

N

N

F

V

P

A

T

V

R

R

Y

N

L

Q

V

L

N

N

D

A

A

G

C
|
C

V

F

L

A

G

K

K

V

P

P

N

L

V

V

Y

S

G

G

S

A

I

A

F

I

R

R

F

M

E

E

G

G

Q

Q

A

I

T

T

V

V

F

F

N

T

Y

Y

E

Q

D

D

G

G

-

E

P

P

N
x
C

Y

Y

R

R

D
x
C

L

L

Y

S

P

R

E

L

P

F

P

G

P

E

P

N

G

A

M

L

V

-

P

-

S

T

C
|
C

A

V

E

E

G

A

G

G

V

V

L

M

G

A

I

P

L

L

P

I

G

G

I

V

I

I

G

G

V

S

I

L

Q

Q

A

A

T

M

E

E

T

A

V

I

K

K

I

M

I

L

L

A

G

G

Q

Y

G

G

Q

K

T

P

L

A

S

S

G

G

R

K

L

I

L

V

L

M

Y

Y

N

D

A

A

L

M

D

T

M
x
C

K

Q

F

F

R

R

E

E

L

M

K

K

L

L

R

M

P

R

N

N

P

P

R14 (= R21) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (≠ S51) mutation to A: No effect.
R73 (= R80) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (≠ G135) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D137) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (= C149) mutation to A: No effect.
C172 (≠ N178) mutation to A: No zinc bound and no enzyme activity.
C175 (≠ D181) mutation to A: No zinc bound and no enzyme activity.
C187 (= C195) mutation to A: No effect.
C231 (≠ M239) mutation to A: No effect.

Sites not aligning to the query:

244 C→A: No zinc bound and almost no enzyme activity.
247 C→A: No zinc bound and almost no enzyme activity.

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
39% identity, 62% coverage: 10:250/391 of query aligns to 2:234/240 of 1jwbB

query
sites
1jwbB

Q

E

L

L

T

S

Q

D

D

Q

D

E

Y

M

A

L

R

R

Y

Y

S

N

R
|
R

H

Q

L

I

I

I

L

L

P

R

E

G

V

F

G

D

L

F

E

D

G

G

Q

Q

K

E

R

A

L

L

K

K

A

D

A

S

K

R

V

V

L

L

C

I

I

V

G
|
G

T

L

G
|
G

L

L

G

G

S

C

P

A

L

A

L

S

L

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

I

V

G

G

R

N

I

L

G

T

I

L

V

L

D
|
D

F
|
F

D

D

I

T

V

V

D

S

H

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

T

I

L

H

H

G

S

T

D

S

A

W

T

V

V

G

G

K

Q

P

P

K
|
K

I

V

E

E

S

S

A

A

K

R

N

D

R

A

I

L

H

T

E

R

I

I

N

N

P

P

Y

H

C

I

Q

A

V

I

D

T

L

P

Y

V

E

N

T

A

A
x
L

L

L

S

D

E

A

N

E

A

L

L

A

S

A

I

L

L

I

E

A

P

E

Y

H

D

D

V

L

I

V

V

L

D

D

G
x
C

T
|
T

D
|
D

N
|
N

F

V

P

A

T
x
V

R

R

Y

N

L

Q

V

L

N

N

D

A

A

G

C

C

V

F

L

A

G

K

K

V

P

P

N

L

V

V

Y

S

G

G

S

A

I

A

F

I

R

R

F

M

E

E

G

G

Q

Q

A

I

T

T

V

V

F

F

N

T

Y

Y

E

Q

D

D

G

G

-

E

P

P

N
x
C

Y

Y

R

R

D

C

L

L

Y

S

P

R

E

L

P

F

P

-

G

-

M

-

V

-

P

-

S

-

C

-

A

G

E

E

G

A

G

G

V

V

L

M

G

A

I

P

L

L

P

I

G

G

I

V

I

I

G

G

V

S

I

L

Q

Q

A

A

T

M

E

E

T

A

V

I

K

K

I

M

I

L

L

A

G

G

Q

Y

G

G

Q

K

T

P

L

A

S

S

G

G

R

K

L

I

L

V

L

M

Y

Y

N

D

A

A

L

M

D

T

M

C

K

Q

F

F

R

R

E

E

L

M

K

K

L

L

R

M

P

R

N

N

P

P

active site: R13 (= R21), D129 (= D137)
binding adenosine monophosphate: G37 (= G45), G39 (= G47), G40 (= G48), D61 (= D69), F62 (= F70), K85 (= K93), L108 (≠ A116), C127 (≠ G135), T128 (= T136), D129 (= D137), N130 (= N138), V133 (≠ T141)
binding zinc ion: C171 (≠ N178)

Sites not aligning to the query:

binding zinc ion: 236, 239

1jw9B Structure of the native moeb-moad protein complex (see paper)
39% identity, 62% coverage: 10:250/391 of query aligns to 2:234/240 of 1jw9B

query
sites
1jw9B

Q

E

L

L

T

S

Q

D

D

Q

D

E

Y

M

A

L

R

R

Y

Y

S

N

R
|
R

H

Q

L

I

I

I

L

L

P

R

E

G

V

F

G

D

L

F

E

D

G

G

Q

Q

K

E

R

A

L

L

K

K

A

D

A

S

K

R

V

V

L

L

C

I

I

V

G

G

T

L

G

G

L

L

G

G

S

C

P

A

L

A

L

S

L

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

I

V

G

G

R

N

I

L

G

T

I

L

V

L

D

D

F

F

D

D

I

T

V

V

D

S

H

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

T

I

L

H

H

G

S

T

D

S

A

W

T

V

V

G

G

K

Q

P

P

K

K

I

V

E

E

S

S

A

A

K

R

N

D

R

A

I

L

H

T

E

R

I

I

N

N

P

P

Y

H

C

I

Q

A

V

I

D

T

L

P

Y

V

E

N

T

A

A

L

L

L

S

D

E

A

N

E

A

L

L

A

S

A

I

L

L

I

E

A

P

E

Y

H

D

D

V

L

I

V

V

L

D

D

G

C

T

T

D
|
D

N

N

F

V

P

A

T

V

R

R

Y

N

L

Q

V

L

N

N

D

A

A

G

C

C

V

F

L

A

G

K

K

V

P

P

N

L

V

V

Y

S

G

G

S

A

I

A

F

I

R

R

F

M

E

E

G

G

Q

Q

A

I

T

T

V

V

F

F

N

T

Y

Y

E

Q

D

D

G

G

-

E

P

P

N
x
C

Y

Y

R

R

D

C

L

L

Y

S

P

R

E

L

P

F

P

-

G

-

M

-

V

-

P

-

S

-

C

-

A

G

E

E

G

A

G

G

V

V

L

M

G

A

I

P

L

L

P

I

G

G

I

V

I

I

G

G

V

S

I

L

Q

Q

A

A

T

M

E

E

T

A

V

I

K

K

I

M

I

L

L

A

G

G

Q

Y

G

G

Q

K

T

P

L

A

S

S

G

G

R

K

L

I

L

V

L

M

Y

Y

N

D

A

A

L

M

D

T

M

C

K

Q

F

F

R

R

E

E

L

M

K

K

L

L

R

M

P

R

N

N

P

P

active site: R13 (= R21), D129 (= D137)
binding zinc ion: C171 (≠ N178)

Sites not aligning to the query:

binding zinc ion: 236, 239

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
45% identity, 54% coverage: 11:223/391 of query aligns to 7:215/289 of 6yubB

query
sites
6yubB

L

L

T

S

Q

K

D

E

Y

I

A

E

R

R

Y

Y

S

S

R

R

H

Q

L

M

I

I

L

V

P

P

E

G

V

M

G

G

L

K

E

E

G

G

Q

Q

K

L

R

R

L

L

K

M

A

N

A

A

K

K

V

V

L

L

C

I

I

I

G

G

T

A

G

G

L

L

G

G

S

C

P

P

L

A

L

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

I

V

G

G

R

T

I

I

G

G

I

I

V

V

D

D

F

G

D

D

I

S

V

V

D

E

H

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

I

A

H

H

G

A

T

T

S

K

W

R

V

V

G

G

K

M

P

L

K

K

I

V

E

D

S

S

A

L

K

I

N

T

R

H

I

L

H

I

E

E

I

I

N

N

P

P

Y

L

C

P

Q

V

V

Y

D

V

L

P

Y

Y

E

R

T

F

A

D

L

L

S

T

S

P

E

Q

N

N

A

A

L

A

S

Q

I

I

L

I

E

K

P

P

Y

W

D

D

V

V

I

I

V

L

D

D

G

C

T

T

D

D

N

N

F

P

P

A

T

T

R

R

Y

Y

L

L

V

I

N

S

D

D

A

V

C

C

V

V

L

L

G

G

K

K

P

P

N

L

V

V

Y

S

G

A

S

A

I

S

F

V

R

Q

F

K

E

S

G

G

Q

Q

A

L

T

I

V

V

F

L

N

N

Y

C

E

-

D

-

G

-

P

-

N

-

Y

-

R

-

D

-

L

-

Y

-

P

-

E

P

P

P

P

T

P

P

P

Q

G

G

M

V

V

V

P

N

S

K

-

K

-

A

-

P

-
x
C

-

Y

-

R

C
|
C

A

C

E

F

G

K

G

G

V

I

L

M

G

G

I

P

L

V

P

V

G

G

I

M

G

G

V

V

I

A

Q

Q

A

A

T

G

E

E

T

A

V

I

K

K

I

I

I

L

L

V

G

S

Q

Q

binding zinc ion: C184 (vs. gap), C187 (= C195)

Sites not aligning to the query:

binding zinc ion: 265

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
36% identity, 61% coverage: 10:246/391 of query aligns to 2:215/217 of 1jwaB

query
sites
1jwaB

Q

E

L

L

T

S

Q

D

D

Q

D

E

Y

M

A

L

R

R

Y

Y

S

N

R
|
R

H

Q

L

I

I

I

L

L

P

R

E

G

V

F

G

D

L

F

E

D

G

G

Q

Q

K

E

R

A

L

L

K

K

A

D

A

S

K

R

V

V

L

L

C

I

I

V

G

G

T

L

G
|
G

L

L

G

G

S

C

P

A

L

A

L

S

L

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

I

V

G

G

R

N

I

L

G

T

I

L

V

L

D
|
D

F
|
F

D

D

I

T

V

V

D

S

H

L

S

S

N

N

L

L

Q

Q

R
|
R

Q

Q

V

T

I

L

H

H

G

S

T

D

S

A

W

T

V

V

G

G

K

Q

P

P

K
|
K

I

V

E

E

S

S

A

A

K

R

N

D

R

A

I

L

H

T

E

R

I

I

N

N

P

P

Y

H

C

I

Q

A

V

I

D

T

L

P

Y

V

E

N

T

A

A
x
L

L

L

S

D

E

A

N

E

A

L

L

A

S

A

I

L

L

I

E

A

P

E

Y

H

D

D

V

L

I

V

V

L

D

D

G

C

T

T

D
|
D

N
|
N

F

V

P

A

T
x
V

R

R

Y

N

L

Q

V

L

N

N

D

A

A

G

C

C

V

F

L

A

G

K

K

V

P

P

N

L

V

V

Y

S

G

G

S

A

I

A

F

I

R

R

F

M

E

E

G

G

Q

Q

A

I

T

T

V

V

F

F

N

T

Y

Y

E

-

D

-

G

-

P

-

N

-

Y

-

R

-

D

-

L

-

Y

-

P

-

E

-

P

-

G

-

M

-

V

-

P

-

S

-

C

-

A

-

E

E

G

A

G

G

V

V

L

M

G

A

I

P

L

L

P

I

G

G

I

V

I

I

G

G

V

S

I

L

Q

Q

A

A

T

M

E

E

T

A

V

I

K

K

I

M

I

L

L

A

G

G

Q

Y

G

G

Q

K

T

P

L

A

S

S

G

G

R

K

L

I

L

V

L

M

Y

Y

N

D

A

A

L

M

D

T

M

C

K

Q

F

F

R

R

E

E

L

M

K

K

L

L

active site: R13 (= R21), D129 (= D137)
binding adenosine-5'-triphosphate: G39 (= G47), G40 (= G48), D61 (= D69), F62 (= F70), R72 (= R80), K85 (= K93), L108 (≠ A116), D129 (= D137), N130 (= N138), V133 (≠ T141)

1zfnA Structural analysis of escherichia coli thif (see paper)
38% identity, 61% coverage: 15:254/391 of query aligns to 5:242/244 of 1zfnA

query
sites
1zfnA

D

D

Y

F

A

M

R

R

Y

Y

S

S

R
|
R

H

Q

L

I

I

L

L

L

P

D

E

D

V

I

G

A

L

L

E

D

G

G

Q

Q

K

Q

R

K

L

L

K

L

A

D

A

S

K

Q

V

V

L

L

C

I

I
|
I

G
|
G

T

L

G
|
G

L

L

G

G

S

T

P

P

L

A

L

L

Y

Y

L

L

A

A

A

G

A

A

G

G

I

V

G

G

R

T

I

L

G

V

I

L

V

A

D
|
D

F

D

D

D

I

D

V

V

D

H

H

L

S

S

N

N

L

L

Q

Q

R
|
R

Q
|
Q

V

I

I

L

H

F

G

T

T

T

S

E

W

D

V

I

G

D

K

R

P

P

K
|
K

I

S

E

Q

S

V

A

S

K

Q

N

Q

R

R

I

L

H

T

E

Q

I

L

N

N

P

P

Y

D

C

I

Q

Q

V

L

D

T

L

A

Y

L

E

Q

T

Q

A

R

L

L

S

T

S

G

E

E

N

A

A

L

L

K

S

D

I

A

L

V

E

A

P

R

Y

A

D

D

V

V

I

V

V

L

D

D

G

C

T
|
T

D
|
D

N

N

F

M

P

A

T
|
T

R

R

Y

Q

L

E

V

I

N

N

D

A

A

A

C

C

V

V

L

A

L

L

G

N

K

T

P

P

N

L

V

I

Y

T

G

A

S

S

I

A

F

V

R

G

F

F

E

G

G

G

Q

Q

A

L

T

M

V

V

F

L

N

T

-

P

Y

W

E

E

D

Q

G

G

P
x
C

N

-

Y

Y

R

R

D
x
C

L

L

Y

W

P

P

E

D

P

N

P

Q

P

E

P

P

G

E

M

R

V

-

P

-

S

N

C

C

A

R

E

T

G

A

G

G

V

V

L

V

G

G

I

P

L

V

P

V

G

G

I

V

I

M

G

G

V

T

I

L

Q

Q

A

A

T

L

E

E

T

A

V

I

K

K

I

L

L

S

G

G

Q

I

G

-

Q

E

T

T

L

P

S

A

G

G

R

E

L

L

L

R

L

L

Y

F

N

D

A

G

L

K

D

S

M

S

K

Q

F

W

R

R

E

S

L

L

K

A

L

L

R

R

P

R

N

A

P

S

E

G

R
x
C

P

P

V

V

active site: R11 (= R21), D127 (= D137)
binding adenosine-5'-triphosphate: I34 (= I44), G35 (= G45), G37 (= G47), G38 (= G48), D59 (= D69), R70 (= R80), Q71 (= Q81), K83 (= K93), T126 (= T136), D127 (= D137), T131 (= T141)
binding zinc ion: C169 (≠ P177), C172 (≠ D181), C240 (≠ R252)

Sites not aligning to the query:

binding zinc ion: 243

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
38% identity, 61% coverage: 15:254/391 of query aligns to 5:242/251 of P30138

query
sites
P30138

D

D

Y

F

A

M

R

R

Y

Y

S

S

R

R

H

Q

L

I

I

L

L

L

P

D

E

D

V

I

G

A

L

L

E

D

G

G

Q

Q

K

Q

R

K

L

L

K

L

A

D

A

S

K

Q

V

V

L

L

C

I

I

I

G

G

T

L

G

G

L

L

G

G

S

T

P

P

L

A

L

L

Y

Y

L

L

A

A

A

G

A

A

G

G

I

V

G

G

R

T

I

L

G

V

I

L

V

A

D

D

F

D

D

D

I

D

V

V

D

H

H

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

I

I

L

H

F

G

T

T

T

S

E

W

D

V

I

G

D

K

R

P

P

K

K

I

S

E

Q

S

V

A

S

K

Q

N

Q

R

R

I

L

H

T

E

Q

I

L

N

N

P

P

Y

D

C

I

Q

Q

V

L

D

T

L

A

Y

L

E

Q

T

Q

A

R

L

L

S

T

S

G

E

E

N

A

A

L

L

K

S

D

I

A

L

V

E

A

P

R

Y

A

D

D

V

V

I

V

V

L

D

D

G

C

T

T

D

D

N

N

F

M

P

A

T

T

R

R

Y

Q

L

E

V

I

N

N

D

A

A

A

C

C

V

V

L

A

L

L

G

N

K

T

P

P

N

L

V

I

Y

T

G

A

S

S

I

A

F

V

R

G

F

F

E

G

G

G

Q

Q

A

L

T

M

V

V

F

L

N

T

-

P

Y

W

E

E

D

Q

G

G

P
x
C

N

-

Y

Y

R

R

D
x
C

L

L

Y
x
W

P

P

E

D

P

N

P

Q

P

E

P

P

G

E

M

R

V

-

P

-

S

N

C
|
C

A

R

E

T

G

A

G

G

V

V

L

V

G

G

I

P

L

V

P

V

G

G

I

V

I

M

G

G

V

T

I

L

Q

Q

A

A

T

L

E

E

T

A

V

I

K

K

I

L

L

S

G

G

Q

I

G

-

Q

E

T

T

L

P

S

A

G

G

R

E

L

L

L

R

L

L

Y

F

N

D

A

G

L

K

D

S

M

S

K

Q

F

W

R

R

E

S

L

L

K

A

L

L

R

R

P

R

N

A

P

S

E

G

R
x
C

P

P

V

V

C169 (≠ P177) binding
C172 (≠ D181) binding
W174 (≠ Y183) mutation to A: No adenylation of ThiS.
C184 (= C195) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.
C240 (≠ R252) binding

Sites not aligning to the query:

243 binding

1zud3 Structure of this-thif protein complex (see paper)
37% identity, 61% coverage: 15:254/391 of query aligns to 5:237/240 of 1zud3

query
sites
1zud3

D

D

Y

F

A

M

R

R

Y

Y

S

S

R
|
R

H

Q

L

I

I
x
L

L

L

P
x
D

E

D

V

I

G

A

L

L

E

D

G

G

Q

Q

K

Q

R

K

L

L

K

L

A

D

A

S

K

Q

V

V

L

L

C

I

I

I

G

G

T

L

G

G

L

L

G

G

S

T

P

P

L

A

L

L

Y

Y

L

L

A

A

A

G

A

A

G

G

I

V

G

G

R

T

I

L

G

V

I

L

V

A

D

D

F

D

D

D

I

D

V

V

D

H

H

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

I

I

L

H

F

G

T

T

T

S

E

W

D

V

I

G

D

K

R

P

P

K

K

I

S

E

Q

S

V

A

S

K

Q

N

Q

R

R

I

L

H

T

E

Q

I

L

N

N

P

P

Y

D

C

I

Q

Q

V

L

D

T

L

A

Y

L

E

Q

T

Q

A

R

L

L

S

T

S

G

E

E

N

A

A

L

L

K

S

D

I

A

L

V

E

A

P

R

Y

A

D

D

V

V

I

V

V

L

D

D

G

C

T

T

D
|
D

N

N

F

M

P

A

T

T

R

R

Y

Q

L

E

V

I

N

N

D

A

A

A

C

C

V

V

L

A

L

L

G

N

K

T

P

P

N

L

V

I

Y

T

G

A

S

S

I

A

F

V

R

G

F

F

E

G

G

G

Q

Q

A

L

T

M

V

V

F

L

N

T

-

P

Y

W

E

E

D

Q

G

G

P

-

N
x
C

Y

Y

R

R

D

C

L

L

Y

W

P

P

E

D

P

N

P

Q

P

E

P

P

G

T

M

A

V

G

P

V

S

V

C

G

A

P

E

V

G

V

G

G

V

V

L

M

G

G

I

T

L

L

P

-

G

-

I

-

G

-

V

-

I

-

Q

Q

A

A

T

L

E

E

T

A

V

I

K

K

I

L

L

S

G

G

Q

I

G

-

Q

E

T

T

L

P

S

A

G

G

R

E

L

L

L

R

L

L

Y

F

N

D

A

G

L

K

D

S

M

S

K

Q

F

W

R

R

E

S

L

L

K

A

L

L

R

R

P

R

N

A

P

S

E

G

R
x
C

P

P

V

V

active site: R11 (= R21), D127 (= D137)
binding calcium ion: L14 (≠ I24), D16 (≠ P26)
binding zinc ion: C169 (≠ N178), C235 (≠ R252)

Sites not aligning to the query:

binding zinc ion: 238

O42939 Ubiquitin-activating enzyme E1-like; Pmt3-activating enzyme subunit 2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 44% coverage: 26:196/391 of query aligns to 13:182/628 of O42939

query
sites
O42939

P

P

E

L

V

T

G

F

L

V

E

E

G

A

Q

L

K

R

R

N

L

F

K

K

A

S

A

A

K

K

V

V

L

L

C

L

I

V

G

G

T

A

G

G

L

I

G

G

S

C

P

E

L

L

L

K

Y

N

L

L

A

L

A

M

A

S

G

G

I

V

G

K

R

E

I

V

G

H

I

I

V

I

D

D

F

L

D

D

I

T

V

I

D

D

H

L

S

S

N

N

L

L

Q

N

R

R

Q

Q

V

F

I

L

H

F

G

R

T

K

S

K

W

H

V

V

G

K

K

Q

P

P

K

K

I

A

E

I

S

V

A

A

K

A

N

K

R

T

I

A

H

S

E

S

I

F

N

N

P

P

Y

N

C

V

Q

K

V

L

D

E

L

A

Y

Y

E

H

T

A

A

N

L

I

S

K

S

E

E

D

N

R

-

F

A

N

L

V

S

A

I

W

L

F

E

R

P

Q

Y

F

D

D

V

L

I

V

V

F

D

N

G

A

T

L

D

D

N

N

F

L

P

D

T

A

R

R

Y

R

L

H

V

V

N

N

D

K

A

Q

C

C

V

L

L

L

L

A

G

S

K

V

P

P

N

L

V

I

Y

E

G

S

S

G

I

T

F

T

R

G

F

F

E

L

G

G

Q

Q

A

V

T

Q

V

V

F

I

N

I

Y

H

E

G

D

K

G

T

P

E

N

C

Y

Y

R

-

D

D

L

C

Y

N

P

P

E

K

P

-

P

E

P

P

G

K

M

T

V

Y

P

P

S

V

C

C

A

T

Sites not aligning to the query:

559 modified: Phosphoserine
563 modified: Phosphoserine

Q19360 NEDD8-activating enzyme E1 catalytic subunit; Ectopic membrane ruffles in embryo protein 1; Ubiquitin-activating enzyme 3 homolog; EC 6.2.1.64 from Caenorhabditis elegans (see paper)
32% identity, 46% coverage: 8:185/391 of query aligns to 14:190/430 of Q19360

query
sites
Q19360

S

S

I

I

Q

R

L

R

T

L

Q

T

D

D

D

R

Y

D

A

S

R

A

Y

Y

S

K

R

V

H

P

L

W

I

F

L

V

P

P

E

-

V

-

G

G

L

P

E

E

G

N

Q

F

K

E

R

A

L

L

K

Q

A

N

A

T

K

K

V

I

L

L

C

V

I

I

G

G

T

A

G

G

L

L

G

G

S

C

P

E

L

L

L

K

Y

N

L

L

A

A

A

L

A

S

G

G

I

F

G

R

R

T

I

I

G

E

I

V

V

I

D

D

F

M

D

D

I

T

V

I

D

D

H

V

S

S

N

N

L

L

Q

N

R

R

Q

Q

V

F

I

L

H

F

G

R

T

E

S

S

W

D

V

V

G

G

K

K

P

S

K

K

I

A

E

E

S

V

A

A

K

A

N

A

R

F

I

V

H

Q

E

Q

I

R

N

V

P

V

Y

G

C

C

Q

Q

V

V

D

T

L

A

Y

H

E

N

T

C

A

R

L

I

S

-

S

E

E

D

N

K

A

G

L

Q

S

E

I

F

L

Y

E

R

P

K

Y

F

D

S

V

I

I

I

V

I

D

C

G

G

T

L

D

D

N

S

F

I

P

P

T

A

R

R

Y

R

L

W

V

I

N

N

D

G

A

M

-

L

C

C

V

D

L

L

L

V

-

L

-

E

-

M

-

A

-

D

G

G

K

K

P

P

N

D

V

E

Y

N

G

T

S

I

I

I

F

P

R

M

F

I

E

D

G

G

Q

G

A

T

T

E

V

G

F

F

N

K

Y

-

E

-

D

-

G

-

P

G

N

N

Y

A

R

R

D

V

L

I

Y

Y

P

P

E

K

Sites not aligning to the query:

262 A→V: In or198; at 25 degrees Celsius, embryonic lethal due to incomplete cytokinesis with ectopic furrow formation and mis-orientation of mitotic spindle. Reduced cul-3 neddylation.

Q7SXG4 SUMO-activating enzyme subunit 2; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; EC 2.3.2.- from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
29% identity, 35% coverage: 36:170/391 of query aligns to 16:151/650 of Q7SXG4

query
sites
Q7SXG4

L

L

K

S

A

S

A

C

K

R

V

V

L

L

C

V

I

V

G

G

T

A

G

G

L

I

G

G

S

C

P

E

L

L

L

K

Y

N

L

L

A

V

A

L

A

T

G

G

I

F

G

K

R

N

I

I

G

E

I

V

V

I

D

D

F

L

D

D

I

T

V

I

D

D

H

V

S

S

N

N

L

L

Q

N

R

R

Q

Q

V

F

I

L

H

F

G

Q

T

K

S

K

W

H

V

V

G

G

K

K

P

S

K

K

I

A

E

Q

S

V

A

A

K

K

N

E

R

S

I

V

H

L

E

R

I

F

N

C

P

P

Y

S

C

A

Q

N

V

I

D

T

L

A

Y

Y

-

H

E

D

T

S

A

I

L

M

S

N

S

P

E

D

N

Y

A

N

L

V

S

E

I

F

L

F

E

R

P

N

Y

F

D

Q

V

L

I

V

V

M

D

N

G

A

T

L

D

D

N

N

F

R

P

A

T

A

R

R

Y

N

L

H

V

V

N

N

D

R

A

M

C

C

V

L

L

A

G

D

K

I

P

P

N

L

V

I

Y

E

G

S

S

G

I

T

F

A

R

G

F

Y

E

L

G

G

Q

Q

A

V

T

T

V

V

Sites not aligning to the query:

599 modified: Phosphoserine

3gznB Structure of nedd8-activating enzyme in complex with nedd8 and mln4924 (see paper)
30% identity, 47% coverage: 33:214/391 of query aligns to 32:192/429 of 3gznB

query
sites
3gznB

Q

Q

K

F

R

L

L

L

K

D

A

T

A

C

K

K

V

V

L

L

C

V

I

I

G

G

T

A

G
|
G

L

L

G

G

S

C

P

E

L

L

L

K

Y

N

L

L

A

A

A

L

A

S

G

G

I

F

G

R

R

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I

I

G

H

I

V

V

I

D
|
D

F
x
M

D
|
D

I

T

V

I

D

D

H

V

S

S

N

N

L

L

Q

N

R

R

Q

Q

V

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S

K

W

D

V

I

G

G

K

R

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P

K

K

I

A

E

E

S

V

A

A

K

A

N

E

R

F

I

L

H

N

E

D

I

R

N

V

P

P

Y

N

C

C

Q

N

V

V

D

V

L

P

Y

H

E

F

T

N

A
x
K

L
x
I

S
x
Q

S

D

E

F

N

N

A

D

L

-

S

T

I

F

L

Y

E

R

P

Q

Y

F

D

H

V

I

I

I

V

V

D

C

G

G

T
x
L

D
|
D

N

S

F

I

P

I

T
x
A

R

R

Y

R

L
x
W

V

I

N

N

D

G

A

M

C

L

V

I

L

-

G

-

K

-

P

-

N

-

V

-

Y

-

G

-

S

-

I

-

F

-

R

-

F

-

E

-

G

-

Q

-

A

-

T

S

V

L

F

L

N

N

Y

Y

E

E

D

D

G

G

P

-

N

-

Y

-

R

-

D

-

L

-

Y

-

P

-

E

V

P

L

P

D

P

P

P

S

G

S

M

I

V

V

P

P

S

-

C

L

A

I

E

D

G

G

V

T

L

E

G

G

-

F

-

K

-

G

-

N

-

A

-

R

-

V

I

I

L

L

P

P

G

G

I

M

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T

G

A

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C

I

I

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E

A
x
C

T

T

active site: D135 (= D137)
binding [(1S,2S,4R)-4-{4-[(1S)-2,3-dihydro-1H-inden-1-ylamino]-7H-pyrrolo[2,3-d]pyrimidin-7-yl}-2-hydroxycyclopentyl]methyl sulfamate: G46 (= G47), G47 (= G48), D68 (= D69), M69 (≠ F70), D70 (= D71), K115 (≠ A116), I116 (≠ L117), Q117 (≠ S118), L134 (≠ T136), D135 (= D137), A139 (≠ T141), W142 (≠ L144)
binding zinc ion: C191 (≠ A213)

Sites not aligning to the query:

active site: 10, 205, 206, 208
binding zinc ion: 332, 335

1tt5B Structure of appbp1-uba3-ubc12n26: a unique e1-e2 interaction required for optimal conjugation of the ubiquitin-like protein nedd8 (see paper)
30% identity, 47% coverage: 33:214/391 of query aligns to 35:195/414 of 1tt5B

query
sites
1tt5B

Q

Q

K
x
F

R

L

L

L

K

D

A

T

A

C

K

K

V

V

L

L

C

V

I

I

G

G

T

A

G

G

L

L

G

G

S

C

P

E

L

L

L

K

Y

N

L

L

A

A

A

L

A

S

G

G

I

F

G

R

R

Q

I

I

G

H

I

V

V

I

D

D

F

M

D

D

I

T

V

I

D

D

H

V

S

S

N

N

L

L

Q

N

R

R

Q

Q

V

F

I

L

H

F

G

R

T

P

S

K

W

D

V

I

G

G

K

R

P

P

K

K

I

A

E

E

S

V

A

A

K

A

N

E

R

F

I

L

H

N

E

D

I

R

N

V

P

P

Y

N

C

C

Q

N

V

V

D

V

L

P

Y

H

E

F

T

N

A

K

L

I

S

Q

S

D

E

F

N

N

A

D

L

-

S

T

I

F

L

Y

E
x
R

P

Q

Y
x
F

D

H

V

I

I

I

V

V

D

C

G

G

T

L

D
|
D

N

S

F

I

P

I

T

A

R

R

Y

R

L

W

V

I

N

N

D

G

A

M

C

L

V

I

L

-

G

-

K

-

P

-

N

-

V

-

Y

-

G

-

S

-

I

-

F

-

R

-

F

-

E

-

G

-

Q

-

A

-

T

S

V

L

F

L

N

N

Y

Y

E

E

D

D

G

G

P

-

N

-

Y

-

R

-

D

-

L

-

Y

-

P

-

E

V

P

L

P

D

P

P

P

S

G

S

M
x
I

V

V

P

P

S

-

C

L

A

I

E

D

G

G

V

T

L

E

G

G

-

F

-

K

-

G

-

N

-

A

-

R

-

V

I

I

L
|
L

P

P

G

G

I
x
M

I

T

G

A

V
x
C

I

I

Q

E

A

C

T

T

active site: D138 (= D137)
binding zinc ion: C191 (≠ V210)
binding : F36 (≠ K34), R128 (≠ E127), F130 (≠ Y129), I166 (≠ M191), L185 (= L204), M188 (≠ I207)

Sites not aligning to the query:

active site: 13, 208, 209, 211
binding zinc ion: 335, 338
binding : 24, 25, 33, 303

Query Sequence

>Synpcc7942_0158 FitnessBrowser__SynE:Synpcc7942_0158
MLNLDTDSIQLTQDDYARYSRHLILPEVGLEGQKRLKAAKVLCIGTGGLGSPLLLYLAAA
GIGRIGIVDFDIVDHSNLQRQVIHGTSWVGKPKIESAKNRIHEINPYCQVDLYETALSSE
NALSILEPYDVIVDGTDNFPTRYLVNDACVLLGKPNVYGSIFRFEGQATVFNYEDGPNYR
DLYPEPPPPGMVPSCAEGGVLGILPGIIGVIQATETVKIILGQGQTLSGRLLLYNALDMK
FRELKLRPNPERPVIDRLIDYQEFCGIPQAQAAEAAATDSIPEISVTELKAILDRQDPEV
LLIDVRNPHEAEIATIPGAILVPLPDIEAGAGVETVRELLNGKRELIVHCKMGGRSAKAL
GILKEAGITGTNVAGGINAWSQDVDPSVPQY

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory