SitesBLAST

P78 (≠ N49) to R: in CBSD; severe form; associated in cis with N-102; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs786204608
G85 (= G56) to R: in CBSD; loss of cystathionine beta-synthase activity; dbSNP:rs863223435
T87 (= T58) to N: in CBSD; decreased cystathionine beta-synthase activity; increased aggregation
L101 (vs. gap) to P: in CBSD; common mutation in Irish population; loss of activity; dbSNP:rs786204757
K102 (vs. gap) to N: in CBSD; associated in cis with R-78; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs786204609; to Q: in dbSNP:rs34040148
C109 (≠ A78) to R: in CBSD; loss of activity; dbSNP:rs778220779
A114 (≠ P83) to V: in CBSD; mild form; when linked with W-58 severe form; decreased cystathionine beta-synthase activity; decreases homotetramer formation by promoting formation of larger aggregates; dbSNP:rs121964964
K119 (= K88) modified: N6-(pyridoxal phosphate)lysine
R125 (≠ F94) to Q: in CBSD; severe form; when linked with D-131 moderate form; loss of cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs781444670; to W: in CBSD; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; dbSNP:rs886057100
M126 (≠ I95) to V: in CBSD; loss of activity
E131 (= E100) to D: in CBSD; linked with Q-125; loss of activity; dbSNP:rs1555875351
G139 (= G108) to R: in CBSD; mild form; dbSNP:rs121964965
I143 (≠ V112) to M: in CBSD; 4% of activity; stable; dbSNP:rs370167302
E144 (= E113) to K: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs121964966
G148 (= G117) to R: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; loss of homotetramer formation; dbSNP:rs755952006
N149 (= N118) binding
L154 (= L123) to Q: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity
A155 (= A124) to T: in CBSD; complete loss of activity; severely affects homotetramer formation by promoting formation of larger aggregates; dbSNP:rs1429138569; to V: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity
C165 (= C134) to Y: in CBSD; severe form; protein expression is comparable to wild-type; loss of cystathionine beta-synthase activity; no effect on homotetramer formation; dbSNP:rs1347651454
M173 (≠ Q142) to V: in CBSD; presents 40% of the wild-type activity; highly reduced capacity to form multimeric quaternary structure; natural variant: Missing (in CBSD; loss of activity)
E176 (= E145) to K: in CBSD; severe form; loss of cystathionine beta-synthase activity; inhibited by AdoMet; severely decreases homotetramer formation by promoting formation of larger aggregates; dbSNP:rs762065361
V180 (≠ L149) to A: in CBSD; decreased cystathionine beta-synthase activity; decreases homotetramer formation; dbSNP:rs1555875010
T191 (= T159) to M: in CBSD; moderate and severe forms; loss of cystathionine beta-synthase activity; absent capacity to form multimeric quaternary structure; dbSNP:rs121964973
K211 (≠ A179) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
A226 (≠ L194) to T: in CBSD; presents 20% of the wild-type activity; dramatically reduced capacity to form multimeric quaternary structure; dbSNP:rs763835246
N228 (= N196) to K: in CBSD; loss of cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs1464223176; to S: in CBSD; has significantly decreased levels of enzyme activity; dbSNP:rs1555874803
A231 (= A199) to P: in CBSD; has significantly decreased levels of enzyme activity
D234 (≠ A202) to N: in CBSD; decreased cystathionine beta-synthase activity; changed localization; decreased interaction with pyridoxal 5'-phosphate; no effect on homotetramer formation; dbSNP:rs773734233; natural variant: Missing (in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity)
GTGGT 256:260 (= GTGGT 224:228) binding
T257 (= T225) to M: in CBSD; moderate to severe form; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs758236584
T262 (≠ A230) to M: in CBSD; moderate form; dbSNP:rs149119723; to R: in CBSD; severe form; loss of cystathionine beta-synthase activity; loss of homotetramer formation
R266 (≠ L234) to K: in CBSD; mild form; decreased cystathionine beta-synthase activity; decreased homotetramer formation; no effect on heme-binding; decreased stability; dbSNP:rs121964969
K269 (= K237) natural variant: Missing (in CBSD; loss of expression)
C272 (≠ S240) mutation to A: Reduced heme content and cystathionine beta-synthase activity.
C275 (≠ V243) to Y: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; mutation to S: Reduced heme content and cystathionine beta-synthase activity.
I278 (≠ V246) to S: in CBSD; loss of activity; to T: in CBSD; mild to severe form; common mutation; decreased expression; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; severely affects homotetramer formation by promoting formation of larger aggregates; dbSNP:rs5742905
D281 (= D249) to N: in CBSD; loss of activity
A288 (≠ Y256) to T: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs141502207
E302 (≠ S270) to K: in CBSD; no effect on cystathionine beta-synthase activity; inhibited by AdoHcy and impaired activation by AdoMet; no effect on homotetramer formation; dbSNP:rs779270933
G305 (= G274) to R: in CBSD; loss of cystathionine beta-synthase activity; no effect on homotetramer formation
G307 (= G276) to S: in CBSD; moderate to severe form; linked with D-534; common mutation; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; no effect on homotetramer formation; dbSNP:rs121964962
V320 (≠ I289) to A: in CBSD; has 36% of wild-type enzyme activity; dbSNP:rs781567152
D321 (= D290) to V: in CBSD; loss of activity
R336 (≠ Y305) to C: in CBSD; protein expression is comparable to wild-type; loss of activity; absent capacity to form multimeric quaternary structure; dbSNP:rs398123151; to H: in CBSD; mild form; no effect on expression; exhibits an activity lower than 4% of the wild-type enzyme; altered stimulation by AdoMet; absent capacity to form multimeric quaternary structure; dbSNP:rs760417941
L338 (= L307) to P: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
G347 (= G316) to S: in CBSD; protein expression is comparable to wild-type; loss of activity; dbSNP:rs771298943
S349 (= S318) binding ; to N: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
T353 (≠ N322) to M: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs121964972
R369 (≠ T338) to C: in CBSD; when linked with C-491 severe form; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs117687681
D376 (= D345) to N: in CBSD; has significantly decreased levels of enzyme activity; dbSNP:rs1170128038
R379 (≠ A348) to Q: in CBSD; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; dbSNP:rs763036586
K384 (≠ R353) to E: in CBSD; severe form; dbSNP:rs121964967

Sites not aligning to the query:

18 R → C: results in 1/3 to 2/3 the enzyme activity of the wild-type; dbSNP:rs201827340
49 P → L: in CBSD; decreased expression; no effect on cystathionine beta-synthase activity; increased homotetramer formation; dbSNP:rs148865119
52 binding axial binding residue
58 R → W: in CBSD; linked with V-114; 18% of activity; dbSNP:rs555959266
65 binding axial binding residue; H → R: in CBSD; decreased cystathionine beta-synthase activity; inhibited by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs1191141364
69 A → P: in dbSNP:rs17849313
422 P → L: in CBSD; changed cystathionine beta-synthase activity; impaired stimulation by AdoMet; does not affect homotetramer formation; dbSNP:rs28934892
427 P → L: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; dbSNP:rs863223434
435 I → T: in CBSD; no effect on cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; does not affect homotetramer formation
439 R → Q: in CBSD; no effect on cystathionine beta-synthase activity; increased homotetramer formation; dbSNP:rs756467921
444 D → N: in CBSD; decreased expression; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; increased homotetramer formation; dbSNP:rs28934891
449 V → G: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet
456 L → P: in CBSD; severe; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
466 S → L: in CBSD; increased cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs121964971
500 S → L: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; dbSNP:rs755106884
526 Q → K: in CBSD; has significantly decreased levels of enzyme activity
539 L → S: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; loss of homotetramer formation; dbSNP:rs121964968
540 L → Q: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet
548 R → Q: presents 60% of the wild-type activity; highly reduced capacity to form multimeric quaternary structure; dbSNP:rs150828989

7qgtB Crystal structure of human cystathionine beta-synthase (delta516-525) in complex with aoaa. (see paper)
41% identity, 85% coverage: 51:365/372 of query aligns to 40:356/500 of 7qgtB

query
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7qgtB

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binding protoporphyrin ix containing fe: A186 (≠ L194), P189 (≠ R197), L190 (≠ Q198), Y193 (= Y201), R226 (≠ L234)
binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: K79 (= K88), T106 (= T115), S107 (≠ A116), N109 (= N118), T110 (= T119), Q182 (= Q190), G216 (= G224), T217 (= T225), G218 (= G226), T220 (= T228), G265 (= G274), S309 (= S318), P335 (≠ C344), D336 (= D345)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 10, 11, 12, 13, 14, 23, 24, 25

7qgtA Crystal structure of human cystathionine beta-synthase (delta516-525) in complex with aoaa. (see paper)
41% identity, 85% coverage: 51:365/372 of query aligns to 40:356/500 of 7qgtA

query
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7qgtA

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binding protoporphyrin ix containing fe: A186 (≠ L194), P189 (≠ R197), L190 (≠ Q198), Y193 (= Y201), R226 (≠ L234)
binding pyridoxal-5'-phosphate: K79 (= K88), N109 (= N118), G216 (= G224), T217 (= T225), G218 (= G226), T220 (= T228), G265 (= G274), S309 (= S318), P335 (≠ C344), D336 (= D345)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 11, 12, 13, 14, 18, 23, 24, 25

P9WP51 Probable cystathionine beta-synthase Rv1077; Beta-thionase; Serine sulfhydrase; EC 4.2.1.22 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
43% identity, 86% coverage: 45:365/372 of query aligns to 1:316/464 of P9WP51

query
sites
P9WP51

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R

H

D

D

P

P

N

A

N

S

Y

Y

V

Y

K

S

V

V

S

S

G

D

R

R

L

L

A

V

E

R

E

D

L

I

D

D

N

G

A

A

I

W

W

K

A

P

N

D

Q

Q

F

Y

D

A

N

N

L

P

A

E

N

G

R

P

Q

A

A

S

H

H

Y

Y

A

V

T

T

G

G

P

P

E

E

I

I

W

W

Q

A

Q

D

T

T

E

E

G

G

T

K

V

V

D

T

A

H

W

F

V

V

A

A

A

G

T

I

G

G

T

T

G

G

T

T

Y

I

A

T

G

G

V

A

A

G

L

R

Y

Y

L

L

K

K

E

E

Q

V

S

S

-

G

S

G

K

R

V

V

R

R

C

I

V

V

V

G

A

A

D

D

P

P

M

E

G

G

S

S

G

-

L

V

Y

Y

S

S

W

G

V

-

K

G

T

A

G

G

E

R

I

P

H

Y

L

L

E

-

G

-

S

-

V

-

T

V

E

E

G

G

I

V

G

G

N

E

S

D

R

F

I

W

T

P

A

A

N

A

M

Y

Q

D

D

P

V

S

P

V

I

P

D

D

D

E

A

I

I

I

Q

A

I

V

A

S

D

D

P

S

E

D

A

S

L

F

E

D

I

M

I

T

Y

R

Q

R

L

L

L

A

H

R

R

E

D

E

G

A

L

M

F

L

M

V

G

G

S

S

V

C

G

G

I

M

N

A

V

V

A

V

A

A

Y

L

R

K

L

V

A

A

K

E

E

E

M

A

G

G

P

P

G

D

H

A

T

L

I

I

V

V

T

V

V

L

L

L

C

P

D

D

G

G

A

R

R

G

Y

Y

Q

M

S

S

R

K

L

I

F

F

N

N

A

D

E

A

W

W

L

M

A

S

S

S

K

Y

G

G

L

F

Sites not aligning to the query:

428 modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7xoyA Cystathionine beta-synthase of mycobacterium tuberculosis in the presence of s-adenosylmethionine and serine. (see paper)
44% identity, 84% coverage: 53:365/372 of query aligns to 7:314/458 of 7xoyA

query
sites
7xoyA

D

E

T

L

I

I

G

G

N

G

T

T

P

P

L

L

I

V

R

R

L

L

N

N

S

S

L

V

S

V

E

P

A

D

T

G

G

A

C

G

N

T

I

V

L

A

G

A

K

K

A

V

E

E

F

Y

L

L

N

N

P

P

G

G

S

S

V

S

K
|
K

D

D

R

R

A

I

A

A

L

V

F

K

I

M

I

I

E

E

D

A

A

A

E

E

R

A

Q

S

G

G

K

Q

L

L

R

K

P

P

G

G

T

T

V

I

V

V

E

E

G

P

T

T

A
x
S

G

G

N
|
N

T
|
T

G

G

I

V

G

G

L

L

A

A

H

L

I

V

C

A

N

Q

A

R

K

R

G

G

Y

Y

R

K

C

C

L

V

I

F

V

V

I

C

P

P

E

D

T

K

Q

V

S

S

Q

E

E

D

K

K

I

R

D

N

L

V

L

L

R

I

T

A

L

Y

G

G

A

A

E

E

V

V

R

V

T

V

V

C

P

P

-

T

A

A

V

V

P

P

Y

P

R

H

D

D

P

P

N

A

N

S

Y

Y

V

Y

K

S

V

V

S

S

G

D

R

R

L

L

A

V

E

R

E

D

L

I

D

D

N

G

A

A

I

W

W

K

A

P

N

D

Q

Q

F

Y

D

A

N

N

L

P

A

E

N

G

R

P

Q

A

A

S

H

H

Y

Y

A

V

T

T

G

G

P

P

E

E

I

I

W

W

Q

A

Q

D

T

T

E

E

G

G

T

K

V

V

D

T

A

H

W

F

V

V

A

A

A

G

T

I

G

G

T
|
T

G

G

G
|
G

T
|
T

Y

I

A

T

G

G

V

A

A

G

L

R

Y

Y

L

L

K

K

E

E

Q

V

S

S

-

G

S

G

K

R

V

V

R

R

C

I

V

V

V

G

A

A

D

D

P

P

M

E

G

G

S

S

G

-

L

V

Y

Y

S

S

W

G

V

-

K

G

T

A

G

G

E

R

I

P

H

Y

L

L

E

-

G

-

S

-

V

-

T

V

E

E

G

G

I

V

G

G

N

E

S

D

R

F

I

W

T

P

A

A

N

A

M

Y

Q

D

D

P

V

S

P

V

I

P

D

D

D

E

A

I

I

I

Q

A

I

V

A

S

D

D

P

S

E

D

A

S

L

F

E

D

I

M

I

T

Y

R

Q

R

L

L

L

A

H

R

R

E

D

E

G

A

L

M

F

L

M

V

G

G

S
|
S

V

C

G

G

I

M

N

A

V

V

A

V

A

A

Y

L

R

K

L

V

A

A

K

E

E

E

M

A

G

G

P

P

G

D

H

A

T

L

I

I

V

V

T

V

V

L

L

L

C
x
P

D

D

G

G

A

R

R

G

Y

Y

Q

M

S

S

R

K

L

I

F

F

N

N

A

D

E

A

W

W

L

M

A

S

S

S

K

Y

G

G

L

F

binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: K42 (= K88), S70 (≠ A116), N72 (= N118), T73 (= T119), T180 (= T225), G182 (= G227), T183 (= T228), S267 (= S318), P293 (≠ C344)

7xnzB Native cystathionine beta-synthase of mycobacterium tuberculosis. (see paper)
44% identity, 84% coverage: 53:365/372 of query aligns to 7:314/458 of 7xnzB

query
sites
7xnzB

D

E

T

L

I

I

G

G

N

G

T

T

P

P

L

L

I

V

R

R

L

L

N

N

S

S

L

V

S

V

E

P

A

D

T

G

G

A

C

G

N

T

I

V

L

A

G

A

K

K

A

V

E

E

F

Y

L

L

N

N

P

P

G

G

S

S

V

S

K
|
K

D

D

R

R

A

I

A

A

L

V

F

K

I

M

I

I

E

E

D

A

A

A

E

E

R

A

Q

S

G

G

K

Q

L

L

R

K

P

P

G

G

T

T

V

I

V

V

E

E

G

P

T

T

A

S

G

G

N

N

T

T

G

G

I

V

G

G

L

L

A

A

H

L

I

V

C

A

N

Q

A

R

K

R

G

G

Y

Y

R

K

C

C

L

V

I

F

V

V

I

C

P

P

E

D

T

K

Q

V

S

S

Q

E

E

D

K

K

I

R

D

N

L

V

L

L

R

I

T

A

L

Y

G

G

A

A

E

E

V

V

R

V

T

V

V

C

P

P

-

T

A

A

V

V

P

P

Y

P

R

H

D

D

P

P

N

A

N

S

Y

Y

V

Y

K

S

V

V

S

S

G

D

R

R

L

L

A

V

E

R

E

D

L

I

D

D

N

G

A

A

I

W

W

K

A

P

N

D

Q

Q

F

Y

D

A

N

N

L

P

A

E

N

G

R

P

Q

A

A

S

H

H

Y

Y

A

V

T

T

G

G

P

P

E

E

I

I

W

W

Q

A

Q

D

T

T

E

E

G

G

T

K

V

V

D

T

A

H

W

F

V

V

A

A

A

G

T

I

G
|
G

T
|
T

G
|
G

T
|
T

Y

I

A

T

G

G

V

A

A

G

L

R

Y

Y

L

L

K

K

E

E

Q

V

S

S

-

G

S

G

K

R

V

V

R

R

C

I

V

V

V

G

A

A

D

D

P

P

M

E

G

G

S

S

G

-

L

V

Y

Y

S

S

W

G

V

-

K

G

T

A

G

G

E

R

I

P

H

Y

L

L

E

-

G

-

S

-

V

-

T

V

E

E

G

G

I

V

G

G

N

E

S

D

R

F

I

W

T

P

A

A

N

A

M

Y

Q

D

D

P

V

S

P

V

I

P

D

D

D

E

A

I

I

I

Q

A

I

V

A

S

D

D

P

S

E

D

A

S

L

F

E

D

I

M

I

T

Y

R

Q

R

L

L

L

A

H

R

R

E

D

E

G

A

L

M

F

L

M

V

G

G

S
|
S

V

C

G

G

I

M

N

A

V

V

A

V

A

A

Y

L

R

K

L

V

A

A

K

E

E

E

M

A

G

G

P

P

G

D

H

A

T

L

I

I

V

V

T

V

V

L

L

L

C
x
P

D
|
D

G

G

A

R

R

G

Y

Y

Q

M

S

S

R

K

L

I

F

F

N

N

A

D

E

A

W

W

L

M

A

S

S

S

K

Y

G

G

L

F

binding pyridoxal-5'-phosphate: K42 (= K88), G179 (= G224), T180 (= T225), G181 (= G226), G182 (= G227), T183 (= T228), S267 (= S318), P293 (≠ C344), D294 (= D345)

6xwlC Cystathionine beta-synthase from toxoplasma gondii (see paper)
37% identity, 86% coverage: 47:365/372 of query aligns to 8:329/477 of 6xwlC

query
sites
6xwlC

I

I

R

L

N

D

G

S

F

V

I

L

D

D

T

A

I

I

G

G

N

N

T

T

P

P

L

M

I

V

R

R

L

M

N

K

S

R

L

L

S

A

E

K

A

V

T

Y

G

G

-

L

-

E

C

C

N

D

I

L

L

L

G

A

K

K

A

C

E

E

F

F

L

M

N

S

P

A

G

G

S

S

V

V

K
|
K

D

D

R

R

A

I

A

G

L

K

F

A

I

M

I

V

E

E

D

K

A

A

E

E

R

R

Q

E

G

G

K

R

L

L

R

K

P

A

G

G

G

D

T

T

V

L

V

I

E

E

G

P

T

T

A

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

H

L

I

A

C

A

N

A

A

V

K

R

G

G

Y

Y

R

R

C

M

L

I

I

V

V

T

I

M

P

P

E

A

T

K

Q

M

S

S

Q

A

E

E

K

K

I

S

D

N

L

I

L

M

R

K

T

C

L

L

G

G

A

A

E

E

-

I

V

V

R

R

T

T

V

P

P

T

A

E

V

A

P

A

Y

W

R

N

D

D

P

E

N

N

N

S

Y

H

V

M

K

G

V

V

S

A

G

A

R

K

L

L

A

Q

E

R

E

E

L

L

D

E

N

N

A

A

I

H

W

I

A

L

N

D

Q

Q

F

Y

D

N

N

N

L

T

A

A

N

N

R

P

Q

M

A

V

H

H

Y

Y

A

D

T

V

T

T

G

A

P

E

E

E

I

I

W

I

Q

T

Q

Q

T

C

E

D

G

G

T

D

V

I

D

D

A

M

W

V

V

V

A

I

A

G

T

A

G
|
G

T
|
T

G
|
G

G

G

T
|
T

Y

I

A

T

G

G

V

I

A

G

L

R

Y

K

L

I

K

K

E

E

Q

R

S

C

S

P

K

K

V

C

R

K

C

V

V

V

V

G

A

V

D

D

P

P

M

K

G

G

S

S

G

I

L

L

Y

A

S

V

W

P

V

D

K

S

T

L

G

N

E

D

I

E

H

K

L

R

E

L

G

Q

S

S

S

Y

V

E

T

V

E

E

G
|
G

I

I

G

G

N

Y

S

D

R

F

I

V

T

P

A

G

N

V

M

L

Q

D

D

R

V

K

P

V

I

V

D

D

D

E

A

W

I

V

Q

K

I

V

A

G

D

D

P

A

E

E

A

S

L

F

E

T

I

T

I

A

Y

R

Q

A

L

I

H

R

R

N

D

E

G

G

L

L

F

F

M

V

G

G

S
|
S

V

S

G

G

I

A

N

N

V

V

A

W

A

G

A

A

Y

L

R

Q

L

A

A

A

K

R

E

Q

M

L

G

K

P

K

G

G

H

Q

T

K

I

C

V

V

T

V

V

L

L

L

C
x
P

D
|
D

G

S

A

R

R

N

Y

Y

Q

M

S

S

R

K

L

F

F

I

N

S

A

D

E

E

W

W

L

M

A

A

S

E

K

H

G

G

L

F

binding pyridoxal-5'-phosphate: K51 (= K88), N81 (= N118), G188 (= G224), T189 (= T225), G190 (= G226), T192 (= T228), G238 (= G274), S282 (= S318), P308 (≠ C344), D309 (= D345)

6xylA Crystal structure of delta466-491 cystathionine beta-synthase from toxoplasma gondii with l-serine (see paper)
37% identity, 86% coverage: 47:365/372 of query aligns to 8:329/468 of 6xylA

query
sites
6xylA

I

I

R

L

N

D

G

S

F

V

I

L

D

D

T

A

I

I

G

G

N

N

T

T

P

P

L

M

I

V

R

R

L

M

N

K

S

R

L

L

S

A

E

K

A

V

T

Y

G

G

-

L

-

E

C

C

N

D

I

L

L

L

G

A

K

K

A

C

E

E

F

F

L

M

N

S

P

A

G

G

S

S

V

V

K
|
K

D

D

R

R

A

I

A

G

L

K

F

A

I

M

I

V

E

E

D

K

A

A

E

E

R

R

Q

E

G

G

K

R

L

L

R

K

P

A

G

G

G

D

T

T

V

L

V

I

E

E

G

P

T

T

A

S

G

G

N

N

T
|
T

G

G

I

I

G

G

L

L

A

A

H

L

I

A

C

A

N

A

A

V

K

R

G

G

Y

Y

R

R

C

M

L

I

I

V

V

T

I

M

P

P

E

A

T

K

Q

M

S

S

Q

A

E

E

K

K

I

S

D

N

L

I

L

M

R

K

T

C

L

L

G

G

A

A

E

E

-

I

V

V

R

R

T

T

V

P

P

T

A

E

V

A

P

A

Y

W

R

N

D

D

P

E

N

N

N

S

Y

H

V

M

K

G

V

V

S

A

G

A

R

K

L

L

A

Q

E

R

E

E

L

L

D

E

N

N

A

A

I

H

W

I

A

L

N

D

Q
|
Q

F

Y

D

N

N

N

L

T

A

A

N

N

R

P

Q

M

A

V

H

H

Y

Y

A

D

T

V

T

T

G

A

P

E

E

E

I

I

W

I

Q

T

Q

Q

T

C

E

D

G

G

T

D

V

I

D

D

A

M

W

V

V

V

A

I

A

G

T

A

G
|
G

T
|
T

G
|
G

G

G

T
|
T

Y

I

A

T

G

G

V

I

A

G

L

R

Y

K

L

I

K

K

E

E

Q

R

S

C

S

P

K

K

V

C

R

K

C

V

V

V

V

G

A

V

D

D

P

P

M

K

G

G

S

S

G

I

L

L

Y

A

S

V

W

P

V

D

K

S

T

L

G

N

E

D

I

E

H

K

L

R

E

L

G

Q

S

S

S

Y

V

E

T

V

E

E

G
|
G

I
|
I

G

G

N
x
Y

S

D

R

F

I

V

T

P

A

G

N

V

M

L

Q

D

D

R

V

K

P

V

I

V

D

D

D

E

A

W

I

V

Q

K

I

V

A

G

D

D

P

A

E

E

A

S

L

F

E

T

I

T

I

A

Y

R

Q

A

L

I

H

R

R

N

D

E

G

G

L

L

F

F

M

V

G

G

S
|
S

V

S

G

G

I

A

N

N

V

V

A

W

A

G

A

A

Y

L

R

Q

L

A

A

A

K

R

E

Q

M

L

G

K

P

K

G

G

H

Q

T

K

I

C

V

V

T

V

V

L

L

L

C
x
P

D
|
D

G

S

A

R

R

N

Y

Y

Q

M

S

S

R

K

L

F

F

I

N

S

A

D

E

E

W

W

L

M

A

A

S

E

K

H

G

G

L

F

binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K51 (= K88), T82 (= T119), Q154 (= Q190), G188 (= G224), T189 (= T225), G190 (= G226), T192 (= T228), G238 (= G274), I239 (= I275), Y241 (≠ N277), S282 (= S318), P308 (≠ C344), D309 (= D345)

6c2zA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the plp-aminoacrylate intermediate (see paper)
37% identity, 87% coverage: 41:365/372 of query aligns to 1:336/345 of 6c2zA

query
sites
6c2zA

T

S

E

E

K

Q

R

Q

M

A

D

D

I

S

R

R

N

H

G

N

F

V

I

I

D

D

T

L

I

V

G

G

N

N

T

T

P

P

L

L

I

I

R

A

L

L

N

K

S

K

L

L

S

P

E

K

A

A

T

L

G

G

C

I

N

K

-

P

-

Q

I

I

L

Y

G

A

K

K

A

L

E

E

F

L

L

Y

N

N

P

P

G

G

S

S

V

I

K
|
K

D

D

R

R

A

I

A

A

L

K

F

S

I

M

I

V

E

E

D

E

A

A

E

E

R

A

Q

S

G

G

K

R

L

I

R

H

P

P

G

S

-

R

G

S

T

T

V

L

V

I

E

E

G

P

T
|
T

A
x
S

G

G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

H

L

I

I

C

G

N

A

A

I

K

K

G

G

Y

Y

R

R

C

T

L

I

I

I

V

T

I

L

P

P

E

E

T

K

Q

M

S

S

Q

N

E

E

K

K

I

V

D

S

L

V

L

L

R

K

T

A

L

L

G

G

A

A

E

E

-

I

V

I

R

R

T

T

V

P

P

T

A

A

V

A

P

A

Y

W

R

D

D

S

P

P

N

E

N

S

Y

H

V

I

K

G

V

V

S

A

G

K

R

K

L

L

A

E

E

K

E

E

L

I

D

P

N

G

A

A

I

V

W

I

A

L

N

D

Q
|
Q

F

Y

D

N

N

N

L

M

A

M

N

N

R

P

Q

E

A

A

H

H

Y

Y

A

F

T

G

T

T

G

G

P

R

E

E

I

I

W

Q

Q

R

Q

Q

T

L

E

E

-

D

-

L

-
x
N

-

L

-

F

G
x
D

T
x
N

V

L

D

R

A

A

W

V

V

V

A

A

A

G

T
x
A

G
|
G

T
|
T

G
|
G

G

G

T
|
T

Y

I

A

S

G

G

V

I

A

S

L

K

Y

Y

L

L

K

K

E

E

Q

Q

S

N

S

D

K

K

V

I

R

Q

C

I

V

V

V

G

A

A

D

D

P

P

M

F

G

G

S

S

G

I

L

L

Y

A

S

Q

W

P

V

E

K

N

T

L

G

N

E

K

I

T

H

D

L

I

E

T

G

D

S

Y

S

K

V

V

T

-

E

E

G
|
G

I

I

G

G

N

Y

S

D

R

F

I

V

T

P

A

Q

N

V

M

L

Q

D

D

R

V

K

P

L

I

I

D

D

D

V

A

W

I

Y

Q

K

I

T

A

D

D

D

P

K

E

P

A

S

L

F

E

K

I

Y

I

A

Y

R

Q

Q

L

L

L

I

H

S

R

N

D

E

G

G

L

V

F

L

M

V

G

G

S
|
S

V

S

G

G

I

S

N

A

V

F

A

T

A

A

A

V

Y

V

R

K

L

Y

A

C

K

E

-

D

-

H

-

P

E

E

M

L

G

T

P

E

G

D

H

D

T

V

I

I

V

V

T

A

V

I

L

F

C
x
P

D
|
D

G

S

G

I

A

R

R

S

Y

Y

Q

L

S

T

R

K

L

F

F

V

N

D

A

D

E

E

W

W

L

L

A

K

S

K

K

N

G

N

L

L

binding calcium ion: N180 (vs. gap), D183 (≠ G214), N184 (≠ T215)
binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K50 (= K88), T78 (= T115), S79 (≠ A116), N81 (= N118), T82 (= T119), Q154 (= Q190), A192 (≠ T223), G193 (= G224), T194 (= T225), G195 (= G226), T197 (= T228), G242 (= G274), S286 (= S318), P315 (≠ C344), D316 (= D345)

6c2qA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the plp-l-serine intermediate (see paper)
37% identity, 87% coverage: 41:365/372 of query aligns to 1:336/345 of 6c2qA

query
sites
6c2qA

T

S

E

E

K

Q

R

Q

M

A

D

D

I

S

R

R

N

H

G

N

F

V

I

I

D

D

T

L

I

V

G

G

N

N

T

T

P

P

L

L

I

I

R

A

L

L

N

K

S

K

L

L

S

P

E

K

A

A

T

L

G

G

C

I

N

K

-

P

-

Q

I

I

L

Y

G

A

K

K

A

L

E

E

F

L

L

Y

N

N

P

P

G

G

S

S

V

I

K
|
K

D

D

R

R

A

I

A

A

L

K

F

S

I

M

I

V

E

E

D

E

A

A

E

E

R

A

Q

S

G

G

K

R

L

I

R

H

P

P

G

S

-

R

G

S

T

T

V

L

V

I

E

E

G

P

T
|
T

A
x
S

G

G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

H

L

I

I

C

G

N

A

A

I

K

K

G

G

Y

Y

R

R

C

T

L

I

I

I

V

T

I

L

P

P

E

E

T

K

Q

M

S

S

Q

N

E

E

K

K

I

V

D

S

L

V

L

L

R

K

T

A

L

L

G

G

A

A

E

E

-

I

V

I

R

R

T

T

V

P

P

T

A

A

V

A

P

A

Y

W

R

D

D

S

P

P

N

E

N

S

Y

H

V

I

K

G

V

V

S

A

G

K

R

K

L

L

A

E

E

K

E

E

L

I

D

P

N

G

A

A

I

V

W

I

A

L

N

D

Q
|
Q

F

Y

D

N

N

N

L

M

A

M

N

N

R

P

Q

E

A

A

H

H

Y

Y

A

F

T

G

T

T

G

G

P

R

E

E

I

I

W

Q

Q

R

Q

Q

T

L

E

E

-

D

-

L

-
x
N

-

L

-

F

G
x
D

T
x
N

V

L

D

R

A

A

W

V

V

V

A

A

A

G

T
x
A

G
|
G

T
|
T

G
|
G

G

G

T
|
T

Y

I

A

S

G

G

V

I

A

S

L

K

Y

Y

L

L

K

K

E

E

Q

Q

S

N

S

D

K

K

V

I

R

Q

C

I

V

V

V

G

A

A

D

D

P

P

M

F

G

G

S

S

G

I

L

L

Y

A

S

Q

W

P

V

E

K

N

T

L

G

N

E

K

I

T

H

D

L

I

E

T

G

D

S

Y

S

K

V

V

T

-

E

E

G
|
G

I

I

G

G

N
x
Y

S

D

R

F

I

V

T

P

A

Q

N

V

M

L

Q

D

D

R

V

K

P

L

I

I

D

D

D

V

A

W

I

Y

Q

K

I

T

A

D

D

D

P

K

E

P

A

S

L

F

E

K

I

Y

I

A

Y

R

Q

Q

L

L

L

I

H

S

R

N

D

E

G

G

L

V

F

L

M

V

G

G

S
|
S

V

S

G

G

I

S

N

A

V

F

A

T

A

A

A

V

Y

V

R

K

L

Y

A

C

K

E

-

D

-

H

-

P

E

E

M

L

G

T

P

E

G

D

H

D

T

V

I

I

V

V

T

A

V

I

L

F

C
x
P

D
|
D

G

S

G

I

A

R

R

S

Y

Y

Q

L

S

T

R

K

L

F

F

V

N

D

A

D

E

E

W

W

L

L

A

K

S

K

K

N

G

N

L

L

binding calcium ion: N180 (vs. gap), D183 (≠ G214), N184 (≠ T215)
binding L-Serine, N-[[3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]-4-pyridinyl]methylene]: K50 (= K88), T78 (= T115), S79 (≠ A116), N81 (= N118), T82 (= T119), Q154 (= Q190), A192 (≠ T223), G193 (= G224), T194 (= T225), G195 (= G226), T197 (= T228), G242 (= G274), Y245 (≠ N277), S286 (= S318), P315 (≠ C344), D316 (= D345)

6c2hA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the catalytic core (see paper)
37% identity, 87% coverage: 41:365/372 of query aligns to 1:336/345 of 6c2hA

query
sites
6c2hA

T

S

E

E

K

Q

R

Q

M

A

D

D

I

S

R

R

N

H

G

N

F

V

I

I

D

D

T

L

I

V

G

G

N

N

T

T

P

P

L

L

I

I

R

A

L

L

N

K

S

K

L

L

S

P

E

K

A

A

T

L

G

G

C

I

N

K

-

P

-

Q

I

I

L

Y

G

A

K

K

A

L

E

E

F

L

L

Y

N

N

P

P

G

G

S

S

V

I

K
|
K

D

D

R

R

A

I

A

A

L

K

F

S

I

M

I

V

E

E

D

E

A

A

E

E

R

A

Q

S

G

G

K

R

L

I

R

H

P

P

G

S

-

R

G

S

T

T

V

L

V

I

E

E

G

P

T

T

A

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

H

L

I

I

C

G

N

A

A

I

K

K

G

G

Y

Y

R

R

C

T

L

I

I

I

V

T

I

L

P

P

E

E

T

K

Q

M

S

S

Q

N

E

E

K

K

I

V

D

S

L

V

L

L

R

K

T

A

L

L

G

G

A

A

E

E

-

I

V

I

R

R

T

T

V

P

P

T

A

A

V

A

P

A

Y

W

R

D

D

S

P

P

N

E

N

S

Y

H

V

I

K

G

V

V

S

A

G

K

R

K

L

L

A

E

E

K

E

E

L

I

D

P

N

G

A

A

I

V

W

I

A

L

N

D

Q

Q

F

Y

D

N

N

N

L

M

A

M

N

N

R

P

Q

E

A

A

H

H

Y

Y

A

F

T

G

T

T

G

G

P

R

E

E

I

I

W

Q

Q

R

Q

Q

T

L

E

E

-

D

-

L

-
x
N

-

L

-

F

G
x
D

T
x
N

V

L

D

R

A

A

W

V

V

V

A

A

A

G

T
x
A

G
|
G

T
|
T

G
|
G

G

G

T
|
T

Y

I

A

S

G

G

V

I

A

S

L

K

Y

Y

L

L

K

K

E

E

Q

Q

S

N

S

D

K

K

V

I

R

Q

C

I

V

V

V

G

A

A

D

D

P

P

M

F

G

G

S

S

G

I

L

L

Y

A

S

Q

W

P

V

E

K

N

T

L

G

N

E

K

I

T

H

D

L

I

E

T

G

D

S

Y

S

K

V

V

T

-

E

E

G
|
G

I

I

G

G

N

Y

S

D

R

F

I

V

T

P

A

Q

N

V

M

L

Q

D

D

R

V

K

P

L

I

I

D

D

D

V

A

W

I

Y

Q

K

I

T

A

D

D

D

P

K

E

P

A

S

L

F

E

K

I

Y

I

A

Y

R

Q

Q

L

L

L

I

H

S

R

N

D

E

G

G

L

V

F

L

M

V

G

G

S
|
S

V

S

G

G

I

S

N

A

V

F

A

T

A

A

A

V

Y

V

R

K

L

Y

A

C

K

E

-

D

-

H

-

P

E

E

M

L

G

T

P

E

G

D

H

D

T

V

I

I

V

V

T

A

V

I

L

F

C
x
P

D
|
D

G

S

G

I

A

R

R

S

Y

Y

Q

L

S

T

R

K

L

F

F

V

N

D

A

D

E

E

W

W

L

L

A

K

S

K

K

N

G

N

L

L

binding calcium ion: N180 (vs. gap), D183 (≠ G214), N184 (≠ T215)
binding pyridoxal-5'-phosphate: K50 (= K88), N81 (= N118), A192 (≠ T223), G193 (= G224), T194 (= T225), G195 (= G226), T197 (= T228), G242 (= G274), S286 (= S318), P315 (≠ C344), D316 (= D345)

5ohxA Structure of active cystathionine b-synthase from apis mellifera (see paper)
40% identity, 87% coverage: 47:368/372 of query aligns to 31:350/488 of 5ohxA

query
sites
5ohxA

I

I

R

M

N

P

G

D

F

I

I

L

D

T

T

A

I

I

G

G

N

Q

T

T

P

P

L

L

I

I

R

K

L

L

N

N

S

N

L

I

S

P

E

K

A

S

T

Y

G

G

-

I

-

K

C

C

N

E

I

I

L

Y

G

A

K

K

A

C

E

E

F

F

L

L

N

N

P

P

G

G

S

S

V

V

K
|
K

D

D

R

R

A

I

A

A

L

Y

F

R

I

M

I

I

E

Q

D

D

A

A

E

E

R

D

Q

K

G

G

K

L

L

L

R

K

P

P

G

G

G

C

T

T

V

I

E

E

G

P

T

T

A

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

H

M

I

A

C

A

N

A

A

V

K

R

G

G

Y

Y

R

K

C

C

L

I

I

I

V

V

I

M

P

P

E

E

T

K

Q

M

S

S

Q

D

E

E

K

K

I

I

D

S

L

T

L

L

R

Y

T

A

L

L

G

G

A

A

E

K

-

I

V

I

R

R

T

T

V

P

P

T

A

E

V

A

P

S

Y

W

R

H

D

S

P

P

N

E

N

A

Y

H

V

I

K

S

V

V

S

A

G

Q

R

K

L

L

A

Q

E

K

E

E

L

I

D

P

N

N

A

S

I

I

W

I

A

L

N

D

Q

Q

F

Y

D

T

N

N

L
x
P

A

G

N

N

R
x
P

Q

L

A

A

H

H

Y
|
Y

A

D

T

Q

T

T

G

A

P

I

E

E

I

I

W

W

Q

K

Q

Q

T

C

E

E

G

G

T

K

V

I

D

D

A

Y

W

L

V

V

A

A

A
x
G

T

A

G
|
G

T
|
T

G
|
G

T
|
T

Y

I

A

S

G

G

V

I

A

G

L
x
R

Y

K

L

L

K

K

E

E

Q

L

S

S

S

P

K

N

V

I

R

K

C

I

V

I

V

A

A

V

D

D

P

P

M

K

G

G

S

S

G

I

L

L

Y

D

S

P

W

-

V

-

K

S

T

S

G

D

E

E

I

V

H

-

L

-

E

-

G

G

S

F

S

Y

V

E

T

V

E

E

G
|
G

I

I

G

G

N

Y

S

D

R

F

I

I

T

P

A

T

N

V

M

L

Q

D

D

R

V

N

P

V

I

I

D

D

D

K

A

W

I

I

Q

K

I

T

A

E

D

D

P

N

E

E

A

S

L

L

E

N

I

A

Y

R

Q

M

L

L

L

I

H

R

R

Q

D

E

G

G

L

L

F

L

M

C

G

G

S
|
S

V

S

G

G

I

A

N

A

V

L

A

I

A

A

Y

L

R

K

L

I

A

A

K

K

E

D

M

I

G

P

P

E

G

E

H

K

T

R

I

M

V

V

T

I

V

I

L

L

C
x
P

D
|
D

G

G

G

I

A

R

R

N

Y

Y

Q

L

S

T

R

K

L

F

F

V

N

S

A

E

E

Y

W

W

L

M

A

E

S

T

K

R

G

G

L

F

Q

L

M

Q

P

P

binding protoporphyrin ix containing fe: P181 (≠ L194), P184 (≠ R197), Y188 (= Y201), R221 (≠ L234)
binding pyridoxal-5'-phosphate: K74 (= K88), N104 (= N118), G209 (≠ A222), G211 (= G224), T212 (= T225), G213 (= G226), G214 (= G227), T215 (= T228), G256 (= G274), S300 (= S318), P326 (≠ C344), D327 (= D345)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 4, 7, 8, 9, 10, 14, 16, 17, 18, 19

6c4pA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the pmp complex (see paper)
38% identity, 87% coverage: 42:365/372 of query aligns to 1:335/344 of 6c4pA

query
sites
6c4pA

E

E

K

Q

R

Q

M

A

D

D

I

S

R

R

N

H

G

N

F

V

I

I

D

D

T

L

I

V

G

G

N

N

T

T

P

P

L

L

I

I

R

A

L

L

N

K

S

K

L

L

S

P

E

K

A

A

T

L

G

G

C

I

N

K

-

P

-

Q

I

I

L

Y

G

A

K

K

A

L

E

E

F

L

L

Y

N

N

P

P

G

G

S

S

V

I

K
|
K

D

D

R

R

A

I

A

A

L

K

F

S

I

M

I

V

E

E

D

E

A

A

E

E

R

A

Q

S

G

G

K

R

L

I

R

H

P

P

G

S

-

R

G

S

T

T

V

L

V

I

E

E

G

P

T

T

A

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

H

L

I

I

C

G

N

A

A

I

K

K

G

G

Y

Y

R

R

C

T

L

I

I

I

V

T

I

L

P

P

E

E

T

K

Q

M

S

S

Q

N

E

E

K

K

I

V

D

S

L

V

L

L

R

K

T

A

L

L

G

G

A

A

E

E

-

I

V

I

R

R

T

T

V

P

P

T

A

A

V

A

P

A

Y

W

R

D

D

S

P

P

N

E

N

S

Y

H

V

I

K

G

V

V

S

A

G

K

R

K

L

L

A

E

E

K

E

E

L

I

D

P

N

G

A

A

I

V

W

I

A

L

N

D

Q

Q

F

Y

D

N

N

N

L

M

A

M

N

N

R

P

Q

E

A

A

H

H

Y

Y

A

F

T

G

T

T

G

G

P

R

E

E

I

I

W

Q

Q

R

Q

Q

T

L

E

E

-

D

-

L

-
x
N

-

L

-

F

G
x
D

T
x
N

V

L

D

R

A

A

W

V

V

V

A

A

A

G

T
x
A

G
|
G

T
|
T

G
|
G

G

G

T
|
T

Y

I

A

S

G

G

V

I

A

S

L

K

Y

Y

L

L

K

K

E

E

Q

Q

S

N

S

D

K

K

V

I

R

Q

C

I

V

V

V

G

A

A

D

D

P

P

M

F

G

G

S

S

G

I

L

L

Y

A

S

Q

W

P

V

E

K

N

T

L

G

N

E

K

I

T

H

D

L

I

E

T

G

D

S

Y

S

K

V

V

T

-

E

E

G
|
G

I

I

G

G

N

Y

S

D

R

F

I

V

T

P

A

Q

N

V

M

L

Q

D

D

R

V

K

P

L

I

I

D

D

D

V

A

W

I

Y

Q

K

I

T

A

D

D

D

P

K

E

P

A

S

L

F

E

K

I

Y

I

A

Y

R

Q

Q

L

L

L

I

H

S

R

N

D

E

G

G

L

V

F

L

M

V

G

G

S
|
S

V

S

G

G

I

S

N

A

V

F

A

T

A

A

A

V

Y

V

R

K

L

Y

A

C

K

E

-

D

-

H

-

P

E

E

M

L

G

T

P

E

G

D

H

D

T

V

I

I

V

V

T

A

V

I

L

F

C
x
P

D
|
D

G

S

G

I

A

R

R

S

Y

Y

Q

L

S

T

R

K

L

F

F

V

N

D

A

D

E

E

W

W

L

L

A

K

S

K

K

N

G

N

L

L

binding calcium ion: N179 (vs. gap), D182 (≠ G214), N183 (≠ T215)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: K49 (= K88), N80 (= N118), A191 (≠ T223), G192 (= G224), T193 (= T225), G194 (= G226), T196 (= T228), G241 (= G274), S285 (= S318), P314 (≠ C344), D315 (= D345)

Q2V0C9 Cystathionine beta-synthase; EC 4.2.1.22 from Apis mellifera (Honeybee) (see paper)
41% identity, 84% coverage: 55:368/372 of query aligns to 43:357/504 of Q2V0C9

query
sites
Q2V0C9

I

I

G

G

N

Q

T

T

P

P

L

L

I

I

R

K

L

L

N

N

S

N

L

I

S

P

E

K

A

S

T

Y

G

G

-

I

-

K

C

C

N

E

I

I

L

Y

G

A

K

K

A

C

E

E

F

F

L

L

N

N

P

P

G

G

S

S

V

V

K
|
K

D

D

R

R

A

I

A

A

L

Y

F

R

I

M

I

I

E

Q

D

D

A

A

E

E

R

D

Q

K

G

G

K

L

L

L

R

K

P

P

G

G

G

C

T

T

V

I

E

E

G

P

T

T

A

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

H

M

I

A

C

A

N

A

A

V

K

R

G

G

Y

Y

R

K

C

C

L

I

I

I

V

V

I

M

P

P

E

E

T

K

Q

M

S

S

Q

D

E

E

K

K

I

I

D

S

L

T

L

L

R

Y

T

A

L

L

G

G

A

A

E

K

-

I

V

I

R

R

T

T

V

P

P

T

A

E

V

A

P

S

Y

W

R

H

D

S

P

P

N

E

N

A

Y

H

V

I

K

S

V

V

S

A

G

Q

R

K

L

L

A

Q

E

K

E

E

L

I

D

P

N

N

A

S

I

I

W

I

A

L

N

D

Q

Q

F

Y

D

T

N

N

L

P

A

G

N

N

R

P

Q

L

A

A

H

H

Y

Y

A

D

T

Q

T

T

G

A

P

I

E

E

I

I

W

W

Q

K

Q

Q

T

C

E

E

G

G

T

K

V

I

D

D

A

Y

W

L

V

V

A

A

A

G

T

A

G
|
G

T
|
T

G
|
G

T
|
T

Y

I

A

S

G

G

V

I

A

G

L

R

Y

K

L

L

K

K

E

E

Q

L

S

S

S

P

K

N

V

I

R

K

C

I

V

I

V

A

A

V

D

D

P

P

M

K

G

G

S

S

G

I

L

L

Y

D

-

P

S

S

W

S

V

D

K

S

T

Q

G

N

E

E

I

V

H

-

L

-

E

-

G

G

S

F

S

Y

V

E

T

V

E

E

G

G

I

I

G

G

N

Y

S

D

R

F

I

I

T

P

A

T

N

V

M

L

Q

D

D

R

V

N

P

V

I

I

D

D

D

K

A

W

I

I

Q

K

I

T

A

E

D

D

P

N

E

E

A

S

L

L

E

N

I

A

Y

R

Q

M

L

L

L

I

H

R

R

Q

D

E

G

G

L

L

F

L

M

C

G

G

S
|
S

V

S

G

G

I

A

N

A

V

L

A

I

A

A

Y

L

R

K

L

I

A

A

K

K

E

D

M

I

G

P

P

E

G

E

H

K

T

R

I

M

V

V

T

I

V

I

L

L

C

P

D

D

G

G

G

I

A

R

R

N

Y

Y

Q

L

S

T

R

K

L

F

F

V

N

S

A

E

E

Y

W

W

L

M

A

E

S

T

K

R

G

G

L

F

Q

L

M

Q

P

P

K78 (= K88) modified: N6-(pyridoxal phosphate)lysine
N108 (= N118) binding
GTGGT 215:219 (= GTGGT 224:228) binding
S307 (= S318) binding

Sites not aligning to the query:

12 binding axial binding residue
23 binding axial binding residue

3pc2A Full length structure of cystathionine beta-synthase from drosophila (see paper)
38% identity, 87% coverage: 44:368/372 of query aligns to 34:360/500 of 3pc2A

query
sites
3pc2A

R

R

M

Q

D

Q

I

I

R

T

N

P

G

N

F

I

I

L

D

E

T

V

I

I

G

G

N

C

T

T

P

P

L

L

I

V

R

K

L

L

N

N

S

N

L

I

S

P

E

A

A

S

T

D

G

G

-

I

-

E

C

C

N

E

I

M

L

Y

G

A

K

K

A

C

E

E

F

F

L

L

N

N

P

P

G

G

S

S

V

V

K
|
K

D

D

R

R

A

I

A

G

L

Y

F

R

I

M

I

V

E

Q

D

D

A

A

E

E

R

E

Q

Q

G

G

K

L

L

L

R

K

P

P

G

G

G

Y

T

T

V

I

E

E

G

P

T

T

A

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

H

M

I

A

C

C

N

A

A

V

K

K

G

G

Y

Y

R

K

C

C

L

I

I

I

V

V

I

M

P

P

E

E

T

K

Q

M

S

S

Q

N

E

E

K

K

I

V

D

S

L

A

L

L

R

R

T

T

L

L

G

G

A

A

E

K

-

I

V

I

R

R

T

T

V

P

P

T

A

E

V

A

P

A

Y

Y

R

D

D

S

P

P

N

E

N

G

Y

L

V

I

K

Y

V

V

S

A

G

Q

R

Q

L

L

A

Q

E

R

E

E

L

T

D

P

N

N

A

S

I

I

W

V

A

L

N

D

Q

Q

F

Y

D

R

N

N

L
x
A

A

G

N

N

R
x
P

Q
x
L

A

A

H

H

Y
|
Y

A

D

T

G

T

T

G

A

P

A

E

E

I

I

W

L

Q

W

Q

Q

T

L

E

D

G

N

T

K

V

V

D

D

A

M

W

I

V

V

A

V

A

S

T
x
A

G
|
G

T
|
T

G
x
A

G

G

T
|
T

Y

I

A

S

G

G

V

I

A

G

L
x
R

Y

K

L

I

K

K

E

E

Q

Q

S

V

S

P

K

S

V

C

R

Q

C

I

V

V

V

G

A

V

D

D

P

P

M

Y

G

G

S

S

G

I

L

L

Y

A

S

R

W

P

V

A

K

E

T

L

G

N

E

K

I

T

H

D

L

V

E

Q

G

F

S

Y

S

E

V

V

T

-

E

E

G
|
G

I

I

G

G

N

Y

S

D

R

F

I

P

T

P

A

T

N

V

M

F

Q

D

D

D

V

T

P

V

I

V

D

D

D

V

A

W

I

T

Q

K

I

I

A

G

D

D

P

S

E

D

A

C

L

F

E

P

I

M

I

S

Y

R

Q

R

L

L

L

N

H

A

R

E

D

E

G

G

L

L

F

L

M

C

G

G

S
|
S

V

S

G

G

I

G

N

A

V

M

A

H

A

A

Y

L

R

E

L

H

A

A

K

R

E

K

M

L

G

K

P

K

G

G

H

Q

T

R

I

C

V

V

T

V

V

I

L

L

C
x
P

D
|
D

G

G

G

I

A

R

R

N

Y

Y

Q

M

S

T

R

K

L

F

F

V

N

S

A

D

E

N

W

W

L

M

A

E

S

A

K

R

G

N

L

F

Q

K

M

E

P

P

active site: K80 (= K88), S310 (= S318)
binding protoporphyrin ix containing fe: A187 (≠ L194), P190 (≠ R197), L191 (≠ Q198), Y194 (= Y201), R227 (≠ L234)
binding pyridoxal-5'-phosphate: K80 (= K88), N110 (= N118), A216 (≠ T223), G217 (= G224), T218 (= T225), A219 (≠ G226), T221 (= T228), G266 (= G274), S310 (= S318), P336 (≠ C344), D337 (= D345)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 12, 13, 14, 15, 16, 24, 25, 26

3pc4A Full length structure of cystathionine beta-synthase from drosophila in complex with serine (see paper)
38% identity, 87% coverage: 44:368/372 of query aligns to 36:362/504 of 3pc4A

query
sites
3pc4A

R

R

M

Q

D

Q

I

I

R

T

N

P

G

N

F

I

I

L

D

E

T

V

I

I

G

G

N

C

T

T

P

P

L

L

I

V

R

K

L

L

N

N

S

N

L

I

S

P

E

A

A

S

T

D

G

G

-

I

-

E

C

C

N

E

I

M

L

Y

G

A

K

K

A

C

E

E

F

F

L

L

N

N

P

P

G

G

S

S

V

V

K
|
K

D

D

R

R

A

I

A

G

L

Y

F

R

I

M

I

V

E

Q

D

D

A

A

E

E

R

E

Q

Q

G

G

K

L

L

L

R

K

P

P

G

G

G

Y

T

T

V

I

E

E

G

P

T
|
T

A
x
S

G

G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

H

M

I

A

C

C

N

A

A

V

K

K

G

G

Y

Y

R

K

C

C

L

I

I

I

V

V

I

M

P

P

E

E

T

K

Q

M

S

S

Q

N

E

E

K

K

I

V

D

S

L

A

L

L

R

R

T

T

L

L

G

G

A

A

E

K

-

I

V

I

R

R

T

T

V

P

P

T

A

E

V

A

P

A

Y

Y

R

D

D

S

P

P

N

E

N

G

Y

L

V

I

K

Y

V

V

S

A

G

Q

R

Q

L

L

A

Q

E

R

E

E

L

T

D

P

N

N

A

S

I

I

W

V

A

L

N

D

Q
|
Q

F

Y

D

R

N

N

L
x
A

A

G

N

N

R
x
P

Q
x
L

A

A

H

H

Y
|
Y

A

D

T

G

T

T

G

A

P

A

E

E

I

I

W

L

Q

W

Q

Q

T

L

E

D

G

N

T

K

V

V

D

D

A

M

W

I

V

V

A

V

A

S

T
x
A

G
|
G

T
|
T

G
x
A

G

G

T
|
T

Y

I

A

S

G

G

V

I

A

G

L
x
R

Y

K

L

I

K

K

E

E

Q

Q

S

V

S

P

K

S

V

C

R

Q

C

I

V

V

V

G

A

V

D

D

P

P

M

Y

G

G

S

S

G

I

L

L

Y

A

S

R

W

P

V

A

K

E

T

L

G

N

E

K

I

T

H

D

L

V

E

Q

G

F

S

Y

S

E

V

V

T

-

E

E

G
|
G

I
|
I

G

G

N
x
Y

S

D

R

F

I

P

T

P

A
x
T

N

V

M

F

Q

D

D

D

V

T

P

V

I

V

D

D

D

V

A

W

I

T

Q

K

I

I

A

G

D

D

P

S

E

D

A

C

L

F

E

P

I

M

I

S

Y

R

Q

R

L

L

L

N

H

A

R

E

D

E

G

G

L

L

F

L

M

C

G

G

S
|
S

V

S

G

G

I

G

N

A

V

M

A

H

A

A

Y

L

R

E

L

H

A

A

K

R

E

K

M

L

G

K

P

K

G

G

H

Q

T

R

I

C

V

V

T

V

V

I

L

L

C
x
P

D
|
D

G

G

G

I

A

R

R

N

Y

Y

Q

M

S

T

R

K

L

F

F

V

N

S

A

D

E

N

W

W

L

M

A

E

S

A

K

R

G

N

L

F

Q

K

M

E

P

P

active site: K82 (= K88), S312 (= S318)
binding protoporphyrin ix containing fe: A189 (≠ L194), P192 (≠ R197), L193 (≠ Q198), Y196 (= Y201), R229 (≠ L234), T276 (≠ A282)
binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: K82 (= K88), T109 (= T115), S110 (≠ A116), N112 (= N118), T113 (= T119), Q185 (= Q190), A218 (≠ T223), G219 (= G224), T220 (= T225), A221 (≠ G226), T223 (= T228), G268 (= G274), I269 (= I275), Y271 (≠ N277), S312 (= S318), P338 (≠ C344), D339 (= D345)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 14, 15, 16, 17, 18, 26, 27, 28

3pc3A Full length structure of cystathionine beta-synthase from drosophila in complex with aminoacrylate (see paper)
38% identity, 87% coverage: 44:368/372 of query aligns to 36:362/504 of 3pc3A

query
sites
3pc3A

R

R

M

Q

D

Q

I

I

R

T

N

P

G

N

F

I

I

L

D

E

T

V

I

I

G

G

N

C

T

T

P

P

L

L

I

V

R

K

L

L

N

N

S

N

L

I

S

P

E

A

A

S

T

D

G

G

-

I

-

E

C

C

N

E

I

M

L

Y

G

A

K

K

A

C

E

E

F

F

L

L

N

N

P

P

G

G

S

S

V

V

K
|
K

D

D

R

R

A

I

A

G

L

Y

F

R

I

M

I

V

E

Q

D

D

A

A

E

E

R

E

Q

Q

G

G

K

L

L

L

R

K

P

P

G

G

G

Y

T

T

V

I

E

E

G

P

T
|
T

A
x
S

G

G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

H

M

I

A

C

C

N

A

A

V

K

K

G

G

Y

Y

R

K

C

C

L

I

I

I

V

V

I

M

P

P

E

E

T

K

Q

M

S

S

Q

N

E

E

K

K

I

V

D

S

L

A

L

L

R

R

T

T

L

L

G

G

A

A

E

K

-

I

V

I

R

R

T

T

V

P

P

T

A

E

V

A

P

A

Y

Y

R

D

D

S

P

P

N

E

N

G

Y

L

V

I

K

Y

V

V

S

A

G

Q

R

Q

L

L

A

Q

E

R

E

E

L

T

D

P

N

N

A

S

I

I

W

V

A

L

N

D

Q
|
Q

F

Y

D

R

N

N

L
x
A

A

G

N

N

R
x
P

Q
x
L

A

A

H

H

Y
|
Y

A

D

T

G

T

T

G

A

P

A

E

E

I

I

W

L

Q

W

Q

Q

T

L

E

D

G

N

T

K

V

V

D

D

A

M

W

I

V

V

A

V

A

S

T
x
A

G
|
G

T
|
T

G
x
A

G

G

T
|
T

Y

I

A

S

G

G

V

I

A

G

L
x
R

Y

K

L

I

K

K

E

E

Q

Q

S

V

S

P

K

S

V

C

R

Q

C

I

V

V

V

G

A

V

D

D

P

P

M

Y

G

G

S

S

G

I

L

L

Y

A

S

R

W

P

V

A

K

E

T

L

G

N

E

K

I

T

H

D

L

V

E

Q

G

F

S

Y

S

E

V

V

T

-

E

E

G
|
G

I
|
I

G

G

N

Y

S

D

R

F

I

P

T

P

A

T

N

V

M

F

Q

D

D

D

V

T

P

V

I

V

D

D

D

V

A

W

I

T

Q

K

I

I

A

G

D

D

P

S

E

D

A

C

L

F

E

P

I

M

I

S

Y

R

Q

R

L

L

L

N

H

A

R

E

D

E

G

G

L

L

F

L

M

C

G

G

S
|
S

V

S

G

G

I

G

N

A

V

M

A

H

A

A

Y

L

R

E

L

H

A

A

K

R

E

K

M

L

G

K

P

K

G

G

H

Q

T

R

I

C

V

V

T

V

V

I

L

L

C
x
P

D
|
D

G

G

G

I

A

R

R

N

Y

Y

Q

M

S

T

R

K

L

F

F

V

N

S

A

D

E

N

W

W

L

M

A

E

S

A

K

R

G

N

L

F

Q

K

M

E

P

P

active site: K82 (= K88), S312 (= S318)
binding protoporphyrin ix containing fe: A189 (≠ L194), P192 (≠ R197), L193 (≠ Q198), Y196 (= Y201), R229 (≠ L234)
binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K82 (= K88), T109 (= T115), S110 (≠ A116), N112 (= N118), T113 (= T119), Q185 (= Q190), A218 (≠ T223), G219 (= G224), T220 (= T225), A221 (≠ G226), T223 (= T228), G268 (= G274), I269 (= I275), S312 (= S318), P338 (≠ C344), D339 (= D345)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 14, 15, 16, 17, 18, 26, 27, 28

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
41% identity, 80% coverage: 54:352/372 of query aligns to 6:297/302 of 2efyA

query
sites
2efyA

T

A

I

I

G

G

N

K

T

T

P

P

L

V

I

V

R

R

L

L

N

A

S

K

L

V

S

V

E

E

A

P

T

D

G

M

C

A

N

E

I

V

L

W

G

V

K

K

A

L

E

E

F

G

L

L

N

N

P

P

G

G

S

S

V

I

K
|
K

D

D

R

R

A

P

A

A

L

W

F

Y

I

M

I

I

E

K

D

D

A

A

E

E

R

E

Q

R

G

G

K

I

L

L

R

R

P

P

G

G

G

S

-

G

-

Q

T

V

V

I

V

V

E

E

G

P

T
|
T

A
x
S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

H

M

I

I

C

A

N

A

A

S

K

R

G

G

Y

Y

R

R

C

L

L

I

I

L

V

T

I

M

P

P

E

A

T

Q

Q

M

S

S

Q

E

E

E

K

R

I

K

D

R

L

V

L

L

R

K

T

A

L

F

G

G

A

A

E

E

V

L

R

-

T

-

V

V

P

L

A

T

V

D

P

P

Y

E

R

R

D

R

P

M

N

L

N

A

Y

A

V

R

K

E

V

E

S

A

G

L

R

R

L

L

A

K

E

E

L

L

D

-

N

G

A

A

I

F

W

M

A

P

N

D

Q
|
Q

F

F

D

K

N

N

L

P

A

A

N

N

R

V

Q

R

A

A

H

H

Y

Y

A

E

T

T

G

G

P

P

E

E

I

L

W

Y

Q

E

Q

A

T

L

E

E

G

G

T

R

V

I

D

D

A

A

W

F

V

V

A
x
Y

A

G

T

S

G
|
G

T
|
T

G
|
G

G

G

T
|
T

Y

I

A

T

G

G

V

V

A

G

L

R

Y

Y

L

L

K

K

E

E

Q

R

S

I

S

P

K

H

V

V

R

K

C

V

V

I

V

A

A

V

D
x
E

P

P

M

A

G

R

S
|
S

G

N

L

V

Y

L

S

S

W

G

V

G

K

K

T

M

G

G

E

Q

I

H

H

G

L

F

E

-

G

-

S

-

V

-

T

-

E

Q

G
|
G

I
x
M

G

G

N
x
P

S

G

R

F

I

I

T

P

A

E

N

N

M

L

Q

D

D

L

V

S

P

L

I

L

D

D

D

G

A

V

I

I

Q

Q

I

V

A

W

D

E

P

E

E

D

A

A

L

F

E

P

I

L

I

A

Y

R

Q

R

L

L

L

A

H

R

R

E

D

E

G

G

L

L

F

F

M

L

G

G

G

M

S
|
S

V

S

G

G

I

G

N

I

V

V

A

W

A

A

Y

L

R

Q

L

V

A

A

K

R

E

E

M

L

G

G

P

P

G

G

H

K

T

R

I

V

V

A

T

C

V

I

L

S

C
x
P

D
|
D

G

G

A

W

R

K

Y

Y

Q

L

S

S

active site: K40 (= K88), S70 (≠ A116), E200 (≠ D249), S204 (= S253), S263 (= S318)
binding 5-oxohexanoic acid: T69 (= T115), G71 (= G117), T73 (= T119), Q141 (= Q190), G175 (= G224), G219 (= G274), M220 (≠ I275), P222 (≠ N277)
binding pyridoxal-5'-phosphate: K40 (= K88), N72 (= N118), Y172 (≠ A221), G175 (= G224), T176 (= T225), G177 (= G226), T179 (= T228), G219 (= G274), S263 (= S318), P289 (≠ C344), D290 (= D345)

2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
41% identity, 80% coverage: 54:352/372 of query aligns to 6:297/302 of 2ecqA

query
sites
2ecqA

T

A

I

I

G

G

N

K

T

T

P

P

L

V

I

V

R

R

L

L

N

A

S

K

L

V

S

V

E

E

A

P

T

D

G

M

C

A

N

E

I

V

L

W

G

V

K

K

A

L

E

E

F

G

L

L

N

N

P

P

G

G

S

S

V

I

K
|
K

D

D

R

R

A

P

A

A

L

W

F

Y

I

M

I

I

E

K

D

D

A

A

E

E

R

E

Q

R

G

G

K

I

L

L

R

R

P

P

G

G

G

S

-

G

-

Q

T

V

V

I

V

V

E

E

G

P

T

T

A
x
S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

H

M

I

I

C

A

N

A

A

S

K

R

G

G

Y

Y

R

R

C

L

L

I

I

L

V

T

I

M

P

P

E

A

T

Q

Q

M

S

S

Q

E

E

E

K

R

I

K

D

R

L

V

L

L

R

K

T

A

L

F

G

G

A

A

E

E

V

L

R

-

T

-

V

V

P

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active site: K40 (= K88), S70 (≠ A116), E200 (≠ D249), S204 (= S253), S263 (= S318)
binding (3s)-3-hydroxybutanoic acid: K40 (= K88), G71 (= G117), T73 (= T119), Q141 (= Q190), G219 (= G274)
binding pyridoxal-5'-phosphate: K40 (= K88), N72 (= N118), Y172 (≠ A221), G173 (≠ A222), G175 (= G224), T176 (= T225), T179 (= T228), G219 (= G274), S263 (= S318), P289 (≠ C344)

Query Sequence

>Synpcc7942_0171 FitnessBrowser__SynE:Synpcc7942_0171
MAARDIHFANRDRRPSPLVGAFLLRVREPKQVGRIKPGCPTEKRMDIRNGFIDTIGNTPL
IRLNSLSEATGCNILGKAEFLNPGGSVKDRAALFIIEDAERQGKLRPGGTVVEGTAGNTG
IGLAHICNAKGYRCLIVIPETQSQEKIDLLRTLGAEVRTVPAVPYRDPNNYVKVSGRLAE
ELDNAIWANQFDNLANRQAHYATTGPEIWQQTEGTVDAWVAATGTGGTYAGVALYLKEQS
SKVRCVVADPMGSGLYSWVKTGEIHLEGSSVTEGIGNSRITANMQDVPIDDAIQIADPEA
LEIIYQLLHRDGLFMGGSVGINVAAAYRLAKEMGPGHTIVTVLCDGGARYQSRLFNAEWL
ASKGLQMPQVTT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory