SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Synpcc7942_0427 Synpcc7942_0427 ATPase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
43% identity, 99% coverage: 4:281/282 of query aligns to 5:278/285 of 4yerA

query
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4yerA

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binding adenosine-5'-diphosphate: F14 (= F13), F17 (≠ R16), A19 (≠ V18), P38 (= P37), N39 (= N38), G40 (= G39), A41 (= A40), G42 (= G41), K43 (= K42), T44 (≠ S43), T45 (= T44), T135 (= T134), F136 (≠ L135), S137 (= S136)

Q9BZC7 ATP-binding cassette sub-family A member 2; ATP-binding cassette transporter 2; ATP-binding cassette 2; EC 7.6.2.- from Homo sapiens (Human) (see paper)
37% identity, 81% coverage: 4:231/282 of query aligns to 2050:2282/2435 of Q9BZC7

query
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Q9BZC7

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Sites not aligning to the query:

271 modified: N5-methylglutamine; Q→R: Abolishes methylation by N6AMT1.

7e7qA Cryo-em structure of human abca4 in atp-bound state (see paper)
36% identity, 87% coverage: 19:262/282 of query aligns to 1679:1928/1958 of 7e7qA

query
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7e7qA

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binding adenosine-5'-triphosphate: N1698 (= N38), G1699 (= G39), A1700 (= A40), G1701 (= G41), K1702 (= K42), T1703 (≠ S43), T1704 (= T44), Q1743 (= Q83), T1794 (= T134), S1796 (= S136), G1798 (= G138), H1852 (= H192)
binding magnesium ion: T1703 (≠ S43), Q1743 (= Q83)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 801, 804, 808, 827, 828, 830, 831, 832, 833, 834, 873, 917, 924, 926, 928, 929, 981, 1671
binding magnesium ion: 833, 873

E9Q876 Glucosylceramide transporter ABCA12; ATP-binding cassette sub-family A member 12; EC 7.6.2.1 from Mus musculus (Mouse) (see 2 papers)
38% identity, 80% coverage: 3:228/282 of query aligns to 1345:1571/2595 of E9Q876

query
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E9Q876

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1388:1461 (vs. 46:117, 28% identical) mutation to M: In a mouse model for harlequin ichthyosis (HI), smooth skin (smsk) mutant mice show a pronounced perinatal lethal skin phenotype in 25% of the offspring and newborn mutant pups die within a few hours after birth, and appear severely dehydrated with dry cracking skin. Smsk homozygous mutants embryos show a normal appearance at 14.5 dpc, but at 16.5 dpc develop a partial absence of normal skin folds around the trunk and limbs, and by 18.5 dpc develop a taut, thick skin and limb contractures.

Sites not aligning to the query:

1996 G→D: In a mouse model for harlequin ichthyosis (HI), homozygous mice are embryonic lethal but occasionally pups are found in the first few hours after birth but die and are severely dehydrated and fail to suckle normally. Homozygous pups show hallmarks of HI desease including hyperkeratosis, abnormal extracellular lipid lamellae and defects in cornified envelope processing. At 14.5 dpc and 15.5 dpc homozygous embryos appear normal; however from 16.5 dpc onwards they are characterized by an absence of normal skin folds around the trunk and limbs. As development progressed, embryos develop a taut, thick epidermis and multiple contractures affecting the limbs. Late stage embryos are smaller.

F1MWM0 Retinal-specific phospholipid-transporting ATPase ABCA4; ATP-binding cassette sub-family A member 4; RIM ABC transporter; RIM protein; RmP; Retinal-specific ATP-binding cassette transporter; EC 7.6.2.1 from Bos taurus (Bovine) (see 2 papers)
38% identity, 84% coverage: 18:254/282 of query aligns to 1952:2188/2281 of F1MWM0

query
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F1MWM0

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L

L

V

L

L

D

D

E

E

P

P

T

T

V

T

G

G

L

M

D

D

P

P

Q

Q

T

A

R

R

R

M

I

L

W

W

E

N

I

T

I

I

R

M

D

G

L

I

N

I

R

R

Q

E

G

G

M

R

T

A

V

V

L

V

L

L

T

T

T

S

H

H

Y

S

M

M

E

E

V

C

E

E

S

A

L

L

C

C

D

T

R

R

I

L

G

A

I

I

L

M

D

V

G

K

G

G

Q

A

L

F

I

Q

A

C

L

L

G

G

T

T

L

I

A

Q

E

H

L

L

R

K

R

S

Q

K

H

F

G

G

E

D

G

G

L

Y

V

I

V

V

K

T

Q

M

K

K

G

I

D

R

R

S

W

P

D

K

Y

D

H

D

F

L

P

P

T

D

E

L

A

G

E

P

A

V

E

E

H

Q

F

F

L

F

E

Q

Sites not aligning to the query:

415 modified: carbohydrate, N-linked (Hex...) asparagine
504 modified: carbohydrate, N-linked (Hex...) asparagine
901 modified: Phosphothreonine; T→A: Decreases expression level. Affects subcellular location.
1185 modified: Phosphoserine; S→A: Does not affect subcellular location. Does not affect expression level. Does not affect ATPase activity. Reduces the stimulating effect of all-trans-retinal on ATP hydrolysis.
1309 Cleavage; by trypsin
1313 modified: Phosphothreonine; T→A: Does not affect subcellular location. Does not affect expression level. Does not affect ATPase activity. Reduces the stimulating effect of all-trans-retinal on ATP hydrolysis.
1317 modified: Phosphoserine; S→A: Does not affect subcellular location. Does not affect expression level. Affects both the basal and stimulated ATPase activity.
1319 modified: Phosphoserine
1455 modified: carbohydrate, N-linked (Hex...) asparagine
1527 modified: carbohydrate, N-linked (Hex...) asparagine
1660 modified: carbohydrate, N-linked (Hex...) asparagine

7e7oA Cryo-em structure of human abca4 in nrpe-bound state (see paper)
38% identity, 77% coverage: 19:234/282 of query aligns to 1720:1935/2003 of 7e7oA

query
sites
7e7oA

V

V

N

D

Q

R

L

L

S

C

F

V

A

G

L

V

A

R

P

P

G

G

E

E

S

C

F

F

G

G

L

L

G

G

P

V

N

N

G

G

A

A

G

G

K

K

S

T

T

T

I

F

R

K

M

M

I

L

T

T

T

G

L

D

T

T

R

T

P

V

S

T

Q

S

G

G

Q

D

I

A

T

T

I

V

A

A

G

G

Y

K

D

S

V

I

Q

L

R

T

D

N

R

I

D

S

R

E

V

V

R

H

S

Q

Q

N

L

M

G

G

V

Y

V

C

L

P

Q

Q

Q

F

V

D

S

A

V

I

K

D

N

E

D

L

F

L

T

T

V

G

W

R

E

E

N

H

L

L

E

Y

Y

L

H

Y

G

A

R

R

L

L

H

R

H

G

I

V

P

P

D

A

R

E

E

E

R

I

Q

E

E

K

R

V

I

A

N

N

R

W

W

S

L

I

D

K

Y

S

V

L

E

G

L

L

S

T

D

V

R

Y

R

A

N

D

D

C

R

L

V

A

Q

G

T

T

L

Y

S

S

G

G

M

N

K

K

R

R

R

K

L

L

Q

S

I

T

A

A

R

I

A

A

L

L

L

I

H

G

E

C

P

P

S

P

I

L

L

V

L

L

D

D

E

E

P

P

T

T

V

T

G

G

L

M

D

D

P

P

Q

Q

T

A

R

R

R

M

I

L

W

W

E

N

I

V

I

I

R

V

D

S

L

I

N

I

R

R

Q

E

G

G

M

R

T

A

V

V

L

V

L

L

T

T

T

S

H

H

Y

S

M

M

E

E

V

C

E

E

S

A

L

L

C

C

D

T

R

R

I

L

G

A

I

I

L

M

D

V

G

K

G

G

Q

A

L

F

I

R

A

C

L

M

G

G

T

T

L

I

A

Q

E

H

L

L

R

K

R

S

Q

K

H

F

G

G

E

D

G

G

L

Y

V

I

V

V

K

T

Q

M

K

K

Sites not aligning to the query:

binding [(2S)-3-[2-[(E)-[(2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohexen-1-yl)nona-2,4,6,8-tetraenylidene]amino]ethoxy-oxidanyl-phosphoryl]oxy-2-[(Z)-octadec-9-enoyl]oxy-propyl] (Z)-octadec-9-enoate: 285, 290, 526, 527, 588, 589, 592, 593, 1327, 1454

7lkpA Structure of atp-free human abca4 (see paper)
38% identity, 77% coverage: 19:234/282 of query aligns to 1648:1863/1941 of 7lkpA

query
sites
7lkpA

V

V

N

D

Q

R

L

L

S

C

F

V

A

G

L

V

A

R

P

P

G

G

E

E

S

C

F

F

G

G

L

L

G

G

P

V

N

N

G

G

A

A

G

G

K

K

S

T

T

T

I

F

R

K

M

M

I

L

T

T

T

G

L

D

T

T

R

T

P

V

S

T

Q

S

G

G

Q

D

I

A

T

T

I

V

A

A

G

G

Y

K

D

S

V

I

Q

L

R

T

D

N

R

I

D

S

R

E

V

V

R

H

S

Q

Q

N

L

M

G

G

V

Y

V

C

L

P

Q

Q

Q

F

V

D

S

A

V

I

K

D

N

E

D

L

F

L

T

T

V

G

W

R

E

E

N

H

L

L

E

Y

Y

L

H

Y

G

A

R

R

L

L

H

R

H

G

I

V

P

P

D

A

R

E

E

E

R

I

Q

E

E

K

R

V

I

A

N

N

R

W

W

S

L

I

D

K

Y

S

V

L

E

G

L

L

S

T

D

V

R

Y

R

A

N

D

D

C

R

L

V

A

Q

G

T

T

L

Y

S

S

G

G

M

N

K

K

R

R

R

K

L

L

Q

S

I

T

A

A

R

I

A

A

L

L

L

I

H

G

E

C

P

P

S

P

I

L

L

V

L

L

D

D

E

E

P

P

T

T

V

T

G

G

L

M

D

D

P

P

Q

Q

T

A

R

R

R

M

I

L

W

W

E

N

I

V

I

I

R

V

D

S

L

I

N

I

R

R

Q

E

G

G

M

R

T

A

V

V

L

V

L

L

T

T

T

S

H

H

Y

S

M

M

E

E

V

C

E

E

S

A

L

L

C

C

D

T

R

R

I

L

G

A

I

I

L

M

D

V

G

K

G

G

Q

A

L

F

I

R

A

C

L

M

G

G

T

T

L

I

A

Q

E

H

L

L

R

K

R

S

Q

K

H

F

G

G

E

D

G

G

L

Y

V

I

V

V

K

T

Q

M

K

K

Sites not aligning to the query:

binding Digitonin: 518, 519, 522, 526, 530, 533, 534, 612, 613, 616, 617, 819, 863, 1493, 1496
binding cholesterol: 9, 10, 13, 21, 29, 560, 617, 621, 1066, 1070, 1072, 1073, 1074, 1076, 1402, 1433, 1433, 1436, 1436, 1483, 1486, 1487, 1491, 1492, 1493, 1495, 1496, 1496, 1499, 1500, 1595, 1599, 1603

P78363 Retinal-specific phospholipid-transporting ATPase ABCA4; ATP-binding cassette sub-family A member 4; RIM ABC transporter; RIM proteinv; RmP; Retinal-specific ATP-binding cassette transporter; Stargardt disease protein; EC 7.6.2.1 from Homo sapiens (Human) (see 41 papers)
36% identity, 84% coverage: 18:254/282 of query aligns to 1954:2190/2273 of P78363

query
sites
P78363

V
x
A

V
|
V

N
x
D

Q
x
R

L
|
L

S
x
C

F
x
V

A
x
G

L
x
V

A
x
R

P
|
P

G
|
G

E
|
E

S
x
C

F
|
F

G
|
G

L
|
L

G
|
G

P
x
V

N
|
N

G
|
G

A
|
A

G
|
G

K
|
K

S
x
T

T
|
T

I
x
F

R
x
K

M
|
M

I
x
L

T
|
T

T
x
G

L
x
D

T
|
T

R
x
T

P
x
V

S
x
T

Q
x
S

G
|
G

Q
x
D

I
x
A

T
|
T

I
x
V

A
|
A

G
|
G

Y
x
K

D
x
S

V
x
I

Q
x
L

R
x
T

D
x
N

R
x
I

D
x
S

R
x
E

V
|
V

R
x
H

S
x
Q

Q
x
N

L
x
M

G
|
G

V
x
Y

V
x
C

L
x
P

Q
|
Q

Q
x
F

V
x
D

S
x
A