SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Synpcc7942_0489 FitnessBrowser__SynE:Synpcc7942_0489 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P30838 Aldehyde dehydrogenase, dimeric NADP-preferring; ALDHIII; Aldehyde dehydrogenase 3; Aldehyde dehydrogenase family 3 member A1; EC 1.2.1.5 from Homo sapiens (Human) (see 4 papers)
49% identity, 96% coverage: 10:448/459 of query aligns to 5:445/453 of P30838

query
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P30838

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S134 (≠ C139) to A: in dbSNP:rs887241
E210 (= E215) active site
C244 (= C249) active site; mutation to S: Abolishes activity.
P329 (= P334) to A: in allele ALDH3A1*2; dbSNP:rs2228100

4l2oA Crystal structure of human aldh3a1 with its selective inhibitor 1-(4- fluorophenyl)sulfonyl-2-methylbenzimidazole
49% identity, 96% coverage: 10:448/459 of query aligns to 4:444/446 of 4l2oA

query
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4l2oA

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active site: N114 (= N120), K137 (= K143), E209 (= E215), C243 (= C249), E333 (= E339), Y412 (= Y418)
binding 1-[(4-fluorophenyl)sulfonyl]-2-methyl-1H-benzimidazole: E61 (≠ S67), Y65 (≠ F71), Y115 (= Y121), N118 (≠ Q124), L119 (= L125), M237 (≠ F243), C243 (= C249), I391 (= I397), I394 (≠ V400), T395 (≠ G401), F401 (= F407), H413 (= H419)
binding nicotinamide-adenine-dinucleotide: T112 (≠ P118), W113 (= W119), N114 (= N120), L119 (= L125), E140 (= E146), V169 (≠ A175), T186 (= T192), G187 (= G193), S188 (≠ G194), V191 (≠ I197), E209 (= E215), L210 (= L216), G211 (= G217), C243 (= C249), H289 (= H295), E333 (= E339), F335 (= F341), F401 (= F407)

4h80A Crystal structure of human aldh3a1 with its isozyme selective inhibitor - n-[4-(4-methylsulfonyl-2-nitroanilino)phenyl]acetamide
49% identity, 96% coverage: 10:448/459 of query aligns to 4:444/446 of 4h80A

query
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4h80A

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D

N

R

D

Q

K

V

V

Q

I

E

K

R

K

V

M

L

I

A

A

E

E

T

T

S

S

A

S

G

G

S

G

V

V

C

A

L

A

N

N

D

D

T

V

I

I

L

V

Q

H

V
x
I

G
x
T

V

L

P

H

D

S

A

L

A

P

F
|
F

G

G

V

V

G

G

P

N

S

S

G

G

M

M

G

G

G

S

Y
|
Y

H

H

G

G

K

K

A

K

S

S

F

F

E

E

T

T

F

F

S

S

H

H

Y

R

K

R

L

S

V

C

L

L

K

V

R

R

P

P

F

L

W

M

L

N

D

D

-

E

-

G

L

L

A

K

L

V

R

R

Y

Y

P

P

active site: N114 (= N120), K137 (= K143), E209 (= E215), C243 (= C249), E333 (= E339), Y412 (= Y418)
binding N-(4-{[4-(methylsulfonyl)-2-nitrophenyl]amino}phenyl)acetamide: E61 (≠ S67), Y65 (≠ F71), Y115 (= Y121), N118 (≠ Q124), W233 (= W239), T242 (= T248), C243 (= C249), V244 (≠ I250), I394 (≠ V400), T395 (≠ G401), F401 (= F407)

3szbA Crystal structure of human aldh3a1 modified with the beta-elimination product of aldi-1; 1-phenyl- 2-propen-1-one (see paper)
49% identity, 96% coverage: 10:448/459 of query aligns to 4:444/447 of 3szbA

query
sites
3szbA

A

S

E

E

T

A

L

V

A

K

A

R

L

A

Q

R

A

A

T

A

F

F

D

S

R

S

G

G

D

R

T

T

R

R

T

P

L

L

A

Q

F

F

R

R

L

I

A

Q

R

Q

L

L

Q

E

D

A

L

L

A

Q

K

R

L

L

V

I

A

Q

D

E

N

Q

E

E

A

Q

E

E

L

L

L

V

Q

G

A

A

L

L

A

A

S

A

D

D

L

L

R

H

K

K

P

N

A

E

L

W

E

N

A

A

Y

Y

A

Y

S

E

E

E

I

V

Y

V

F

Y

V

V

R

L

D

E

Q

E

I

I

K

E

L

Y

T

M

C

I

K

Q

H

K

L

L

R

P

R

E

W

W

M

A

Q

A

P

D

E

E

K

P

Q

V

S

E

I

K

S

T

L

P

M

Q

Q

T

Q

Q

P

Q

G

D

Q

E

A

L

Y

Y

R

I

Q

H

A

S

E

E

P

P

L

L

G

G

V

V

L

L

I

V

I

I

G

G

P

T

W

W

N
|
N

Y
|
Y

P

P

F

F

Q

N

L
|
L

L

T

I

I

T

Q

P

P

L

M

I

V

G

G

A

A

I

I

A

A

G

G

N

N

C

A

A

V

V

V

L

L

K
|
K

P

P

S

S

E

E

L

L

A

S

P

E

A

N

T

M

S

A

S

S

L

L

I

L

Q

A

R

T

L

I

I

I

S

P

D

Q

R

Y

F

L

D

D

P

K

D

D

Y

L

I

Y

R

P

V

V

L

I

E

N

G

G

D

G

A

V

S

P

V

E

S

T

Q

T

A

E

L

L

I

L

T

K

Q

E

P

R

F

F

D

D

H

H

I

I

F

L

F

Y

T

T

G

G

G

S

T

T

A

G

I

V

G

G

R

K

K

I

V

I

M

M

A

T

A

A

E

K

N

H

L

L

T

T

P

P

V

V

T

T

L

L

E
|
E

L

L

G

G

K

K

S

S

P

P

C

C

I

Y

V

V

D

D

T

K

D

N

I

C

D

D

L

L

D

D

V

V

A

A

A

C

R

R

I

I

A

A

W

W

G

G

K

K

F

F

F

M

N

N

A

S

G

G

Q

Q

T

T

C
|
C

I

V

A

A

P

P

D

D

Y

Y

L

I

L

L

V

C

Q

D

R

P

T

S

V

I

A

Q

E

N

P

Q

F

I

I

V

E

E

A

K

L

L

I

K

D

K

N

S

I

L

Q

K

Q

E

F

F

Y

Y

G

G

E

E

D

D

P

A

Q

K

S

S

A

R

D

D

Y

Y

A

G

R

R

I

I

V

I

S

S

D

A

R

R

H

H

W

F

Q

Q

R

R

L

V

N

M

S

G

L

L

L

I

V

E

D

G

G

Q

T

K

I

V

R

A

H

Y

G

G

Q

T

V

G

D

D

R

A

S

A

D

T

R

R

Y

Y

I

I

A

A

P

P

T

T

L

I

I

L

T

T

D

D

V

V

N

D

W

P

R

Q

D

S

P

P

I

V

L

M

Q

Q

E

E

E
|
E

I

I

F

F

G

G

P

P

L

V

L

L

P

P

I

I

L

V

I

C

Y

V

D

R

Q

S

L

L

D

E

E

E

A

A

I

I

A

Q

Q

F

I

I

R

N

A

Q

Q

R

P

E

K

K

P

P

L

L

A

A

L

L

Y

Y

L

M

F

F

S

S

R

S

D

N

R

D

Q

K

V

V

Q

I

E

K

R

K

V

M

L

I

A

A

E

E

T

T

S

S

A

S

G

G

S

G

V

V

C

A

L

A

N

N

D

D

T

V

I

I

L

V

Q

H

V
x
I

G

T

V

L

P

H

D

S

A

L

A

P

F

F

G

G

V

V

G

G

P

N

S

S

G

G

M

M

G

G

G

S

Y
|
Y

H

H

G

G

K

K

A

K

S

S

F

F

E

E

T

T

F

F

S

S

H

H

Y

R

K

R

L

S

V

C

L

L

K

V

R

R

P

P

F

L

W

M

L

N

D

D

-

E

-

G

L

L

A

K

L

V

R

R

Y

Y

P

P

active site: N114 (= N120), K137 (= K143), E209 (= E215), C243 (= C249), E333 (= E339), Y412 (= Y418)
binding 1-phenylpropan-1-one: Y115 (= Y121), L119 (= L125), C243 (= C249), I394 (≠ V400)

8bb8A Crystal structure of human aldehyde dehydrogenase aldh3a1 in complex with octanal (see paper)
49% identity, 96% coverage: 10:448/459 of query aligns to 4:444/447 of 8bb8A

query
sites
8bb8A

A

S

E

E

T

A

L

V

A

K

A

R

L

A

Q

R

A

A

T

A

F

F

D

S

R

S

G

G

D

R

T

T

R

R

T

P

L

L

A

Q

F

F

R

R

L

I

A

Q

R

Q

L

L

Q

E

D

A

L

L

A

Q

K

R

L

L

V

I

A

Q

D

E

N

Q

E

E

A

Q

E

E

L

L

L

V

Q

G

A

A

L

L

A

A

S

A

D

D

L

L

R

H

K

K

P

N

A

E

L

W

E

N

A

A

Y

Y

A

Y

S

E

E

E

I

V

Y

V

F
x
Y

V

V

R

L

D

E

Q

E

I

I

K

E

L

Y

T

M

C

I

K

Q

H

K

L

L

R

P

R

E

W

W

M

A

Q

A

P

D

E

E

K

P

Q

V

S

E

I

K

S

T

L

P

M

Q

Q

T

Q

Q

P

Q

G

D

Q

E

A

L

Y

Y

R

I

Q

H

A

S

E

E

P

P

L

L

G

G

V

V

L

L

I

V

I

I

G

G

P

T

W

W

N

N

Y
|
Y

P

P

F

F

Q
x
N

L

L

L

T

I

I

T

Q

P

P

L

M

I

V

G

G

A

A

I

I

A

A

G

G

N

N

C

S

A

V

V

V

L

L

K

K

P

P

S

S

E

E

L

L

A

S

P

E

A

N

T

M

S

A

S

S

L

L

I

L

Q

A

R

T

L

I

I

I

S

P

D

Q

R

Y

F

L

D

D

P

K

D

D

Y

L

I

Y

R

P

V

V

L

I

E

N

G

G

D

G

A

V

S

P

V

E

S

T

Q

T

A

E

L

L

I

L

T

K

Q

E

P

R

F

F

D

D

H

H

I

I

F

L

F

Y

T

T

G

G

G

S

T

T

A

G

I

V

G

G

R

K

K

I

V

I

M

M

A

T

A

A

E

K

N

H

L

L

T

T

P

P

V

V

T

T

L

L

E

E

L

L

G

G

K

K

S

S

P

P

C

C

I

Y

V

V

D

D

T

K

D

N

I

C

D

D

L

L

D

D

V

V

A

A

A

C

R

R

I

I

A

A

W

W

G

G

K

K

F

F

F

M

N

N

A

S

G

G

Q

Q

T

T

C
|
C

I

V

A

A

P

P

D

D

Y

Y

L

I

L

L

V

C

Q

D

R

P

T

S

V

I

A

Q

E

N

P

Q

F

I

I

V

E

E

A

K

L

L

I

K

D

K

N

S

I

L

Q

K

Q

E

F

F

Y

Y

G

G

E

E

D

D

P

A

Q

K

S

S

A

R

D

D

Y

Y

A

G

R

R

I

I

V

I

S

S

D

A

R

R

H

H

W

F

Q

Q

R

R

L

V

N

M

S

G

L

L

L

I

V

E

D

G

G

Q

T

K

I

V

R

A

H

Y

G

G

Q

T

V

G

D

D

R

A

S

A

D

T

R

R

Y

Y

I

I

A

A

P

P

T

T

L

I

I

L

T

T

D

D

V

V

N

D

W

P

R

Q

D

S

P

P

I

V

L

M

Q

Q

E

E

I

I

F

F

G

G

P

P

L

V

L

L

P

P

I

I

L

V

I

C

Y

V

D

R

Q

S

L

L

D

E

E

E

A

A

I

I

A

Q

Q

F

I

I

R

N

A

Q

Q

R

P

E

K

K

P

P

L

L

A

A

L

L

Y

Y

L

M

F

F

S

S

R

S

D

N

R

D

Q

K

V

V

Q

I

E

K

R

K

V

M

L

I

A

A

E

E

T

T

S

S

A

S

G

G

S

G

V

V

C

A

L

A

N

N

D

D

T

V

I

I

L

V

Q

H

V
x
I

G
x
T

V

L

P

H

D

S

A

L

A

P

F

F

G

G

V

V

G

G

P

N

S

S

G

G

M

M

G

G

G

S

Y

Y

H
|
H

G

G

K

K

A

K

S

S

F

F

E

E

T

T

F

F

S

S

H

H

Y

R

K

R

L

S

V

C

L

L

K

V

R

R

P

P

F

L

W

M

L

N

D

D

-

E

-

G

L

L

A

K

L

V

R

R

Y

Y

P

P

binding octanal: Y65 (≠ F71), Y115 (= Y121), N118 (≠ Q124), C243 (= C249), I394 (≠ V400), T395 (≠ G401), H413 (= H419)

4l1oB Crystal structure of human aldh3a1 with inhibitor 1-{[4-(1,3- benzodioxol-5-ylmethyl)piperazin-1-yl]methyl}-1h-indole-2,3-dione (see paper)
49% identity, 96% coverage: 10:448/459 of query aligns to 4:444/452 of 4l1oB

query
sites
4l1oB

A

S

E

E

T

A

L

V

A

K

A

R

L

A

Q

R

A

A

T

A

F

F

D

S

R

S

G

G

D

R

T

T

R

R

T

P

L

L

A

Q

F

F

R

R

L

I

A

Q

R

Q

L

L

Q

E

D

A

L

L

A

Q

K

R

L

L

V

I

A

Q

D

E

N

Q

E

E

A

Q

E

E

L

L

L

V

Q

G

A

A

L

L

A

A

S

A

D

D

L

L

R

H

K

K

P

N

A

E

L

W

E

N

A

A

Y

Y

A

Y

S

E

E

E

I

V

Y

V

F

Y

V

V

R

L

D

E

Q

E

I

I

K

E

L

Y

T

M

C

I

K

Q

H

K

L

L

R

P

R

E

W

W

M

A

Q

A

P

D

E

E

K

P

Q

V

S

E

I

K

S

T

L

P

M

Q

Q

T

Q

Q

P

Q

G

D

Q

E

A

L

Y

Y

R

I

Q

H

A

S

E

E

P

P

L

L

G

G

V

V

L

L

I

V

I

I

G

G

P

T

W

W

N
|
N

Y
|
Y

P

P

F

F

Q
x
N

L
|
L

L

T

I

I

T

Q

P

P

L

M

I

V

G

G

A

A

I

I

A

A

G

G

N

N

C

A

A

V

V

V

L

L

K
|
K

P

P

S

S

E

E

L

L

A

S

P

E

A

N

T

M

S

A

S

S

L

L

I

L

Q

A

R

T

L

I

I

I

S

P

D

Q

R

Y

F

L

D

D

P

K

D

D

Y

L

I

Y

R

P

V

V

L

I

E

N

G

G

D

G

A

V

S

P

V

E

S

T

Q

T

A

E

L

L

I

L

T

K

Q

E

P

R

F

F

D

D

H

H

I

I

F

L

F

Y

T

T

G

G

G

S

T

T

A

G

I

V

G

G

R

K

K

I

V

I

M

M

A

T

A

A

E

K

N

H

L

L

T

T

P

P

V

V

T

T

L

L

E
|
E

L

L

G

G

K

K

S

S

P

P

C

C

I

Y

V

V

D

D

T

K

D

N

I

C

D

D

L

L

D

D

V

V

A

A

A

C

R

R

I

I

A

A

W

W

G

G

K

K

F

F

F

M

N

N

A

S

G

G

Q

Q

T
|
T

C
|
C

I

V

A

A

P

P

D

D

Y

Y

L

I

L

L

V

C

Q

D

R

P

T

S

V

I

A

Q

E

N

P

Q

F

I

I

V

E

E

A

K

L

L

I

K

D

K

N

S

I

L

Q

K

Q

E

F

F

Y

Y

G

G

E

E

D

D

P

A

Q

K

S

S

A

R

D

D

Y

Y

A

G

R

R

I

I

V

I

S

S

D

A

R

R

H

H

W

F

Q

Q

R

R

L

V

N

M

S

G

L

L

L

I

V

E

D

G

G

Q

T

K

I

V

R

A

H

Y

G

G

Q

T

V

G

D

D

R

A

S

A

D

T

R

R

Y

Y

I

I

A

A

P

P

T

T

L

I

I

L

T

T

D

D

V

V

N

D

W

P

R

Q

D

S

P

P

I

V

L

M

Q

Q

E

E

E
|
E

I

I

F

F

G

G

P

P

L

V

L

L

P

P

I

I

L

V

I

C

Y

V

D

R

Q

S

L

L

D

E

E

E

A

A

I

I

A

Q

Q

F

I

I

R

N

A

Q

Q

R

P

E

K

K

P

P

L

L

A

A

L

L

Y

Y

L

M

F

F

S

S

R

S

D

N

R

D

Q

K

V

V

Q

I

E

K

R

K

V

M

L

I

A

A

E

E

T

T

S

S

A

S

G

G

S

G

V

V

C

A

L

A

N

N

D

D

T

V

I
|
I

L

V

Q

H

V
x
I

G

T

V

L

P

H

D

S

A

L

A

P

F
|
F

G

G

V

V

G

G

P

N

S

S

G

G

M

M

G

G

G

S

Y
|
Y

H
|
H

G

G

K

K

A

K

S

S

F

F

E

E

T

T

F

F

S

S

H

H

Y

R

K

R

L

S

V

C

L

L

K

V

R

R

P

P

F

L

W

M

L

N

D

D

-

E

-

G

L

L

A

K

L

V

R

R

Y

Y

P

P

active site: N114 (= N120), K137 (= K143), E209 (= E215), C243 (= C249), E333 (= E339), Y412 (= Y418)
binding (3S)-1-{[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]methyl}-3-hydroxy-1,3-dihydro-2H-indol-2-one: Y115 (= Y121), N118 (≠ Q124), L119 (= L125), E209 (= E215), T242 (= T248), C243 (= C249), I391 (= I397), I394 (≠ V400), F401 (= F407), H413 (= H419)

1ad3A Class 3 aldehyde dehydrogenase complex with nicotinamide-adenine- dinucleotide (see paper)
48% identity, 96% coverage: 10:448/459 of query aligns to 3:443/446 of 1ad3A

query
sites
1ad3A

A

S

E

D

T

T

L

V

A

K

A

R

L

A

Q

R

A

E

T

A

F

F

D

N

R

S

G

G

D

K

T

T

R

R

T

S

L

L

A

Q

F

F

R

R

L

I

A

Q

R

Q

L

L

Q

E

D

A

L

L

A

Q

K

R

L

M

V

I

A

N

D

E

N

N

E

L

A

K

E

S

L

I

L

S

Q

G

A

A

L

L

A

A

S

S

D

D

L

L

R

G

K

K

P

N

A

E

L

W

E

T

A

S

Y

Y

A

Y

S

E

E

E

I

V

Y

A

F

H

V

V

R

L

D

E

Q

E

I

L

K

D

L

T

T

T

C

I

K

K

H

E

L

L

R

P

R

D

W

W

M

A

Q

E

P

D

E

E

K

P

Q

V

S

A

I

K

S

T

L

R

M

Q

Q

T

Q

Q

P

Q

G

D

Q

D

A

L

Y

Y

R

I

Q

H

A

S

E

E

P

P

L

L

G

G

V

V

L

L

I

V

I

I

G

G

P
x
A

W
|
W

N
|
N

Y

Y

P

P

F

F

Q

N

L

L

L

T

I

I

T

Q

P

P

L

M

I

V

G

G

A

A

I

V

A

A

G

G

N

N

C

A

A

V

V

I

L

L

K
|
K

P

P

S

S

E
|
E

L
x
V

A

S

P

G

A

H

T

M

S

A

S

D

L

L

I

L

Q

A

R

T

L

L

I

I

S

P

D

Q

R

Y

F

M

D

D

P

Q

D

N

Y

L

I

Y

R

L

V

V

L

V

E

K

G

G

D

G

A
x
V

S

P

V

E

S

T

Q

T

A

E

L

L

I

L

T

K

Q

E

P

R

F

F

D

D

H

H

I

I

F

M

F

Y

T

T

G
|
G

G

S

T

T

A

A

I
x
V

G

G

R

K

K

I

V

V

M

M

A

A

E

K

N

H

L

L

T

T

P

P

V

V

T

T

L

L

E
|
E

L

L

G

G

K

K

S

S

P

P

C

C

I

Y

V

V

D

D

T

K

D

D

I

C

D

D

L

L

D

D

V

V

A

A

A

C

R

R

I

I

A

A

W

W

G

G

K

K

F

F

F

M

N

N

A

S

G

G

Q

Q

T

T

C
|
C

I

V

A

A

P

P

D

D

Y

Y

L

I

L

L

V

C

Q

D

R

P

T

S

V

I

A

Q

E

N

P

Q

F

I

I

V

E

E

A

K

L

L

I

K

D

K

N

S

I

L

Q

K

Q

D

F

F

Y

Y

G

G

E

E

D

D

P

A

Q

K

Q

Q

S

S

A

R

D

D

Y

Y

A

G

R

R

I

I

V

I

S

N

D

D

R

R

H
|
H

W

F

Q

Q

R
|
R

L

V

N

K

S

G

L

L

L

I

V

D

D

N

G

Q

T

K

I

V

R

A

H

H

G

G

Q

T

V

W

D

D

R

Q

S

S

D

S

R

R

Y

Y

I

I

A

A

P

P

T

T

L

I

I

L

T

V

D

D

V

V

N

D

W

P

R

Q

D

S

P

P

I

V

L

M

Q

Q

E

E

E
|
E

I

I

F
|
F

G

G

P

P

L

V

L

M

P

P

I

I

L

V

I

C

Y

V

D

R

Q

S

L

L

D

E

E

E

A

A

I

I

A

Q

Q

F

I

I

R

N

A

Q

Q

R

P

E

K

K

P

P

L

L

A

A

L

L

Y

Y

L

V

F

F

S

S

R

N

D

N

R

E

Q

K

V

V

Q

I

E

K

R

K

V

M

L

I

A

A

E

E

T

T

S

S

A

S

G

G

S

G

V

V

C

T

L

A

N

N

D

D

T

V

I

I

L

V

Q

H

V

I

G

T

V

V

P

P

D

T

A

L

A

P

F

F

G

G

V

V

G

G

P

N

S

S

G

G

M

M

G

G

G

A

Y
|
Y

H

H

G

G

K

K

A

K

S

S

F

F

E

E

T

T

F

F

S

S

H

H

Y

R

K

R

L

S

V

C

L

L

K

V

R

K

P

S

F

L

W

L

L

N

D

E

L

E

A

A

-

H

-

K

L

A

R

R

Y

Y

P

P

active site: N113 (= N120), K136 (= K143), E208 (= E215), C242 (= C249), E332 (= E339), Y411 (= Y418)
binding nicotinamide-adenine-dinucleotide: A111 (≠ P118), W112 (= W119), N113 (= N120), E139 (= E146), V140 (≠ L147), V168 (≠ A175), G186 (= G193), V190 (≠ I197), H288 (= H295), R291 (= R298), E332 (= E339), F334 (= F341)

Q70DU8 Aldehyde dehydrogenase family 3 member H1; AtALDH4; Ath-ALDH4; EC 1.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
48% identity, 98% coverage: 9:459/459 of query aligns to 12:468/484 of Q70DU8

query
sites
Q70DU8

A

A

A

S

E

N

T

L

L

V

A

T

A

E

L

L

Q

R

A

R

T

S

F

F

D

D

R

D

G

G

D

V

T

T

R

R

T

G

L

Y

A

E

F

W

R

R

L

V

A

T

R

Q

L

L

Q

K

D

K

L

L

A

M

K

I

L

I

V
x
C

A

D

D

N

N

H

E

E

A

P

E

E

L

I

L

V

Q

A

A

A

L

L

A

R

S

D

D

D

L

L

R

G

K

K

P

P

A

E

L

L

E

E

A

S

Y

S

A

V

S

Y

E

E

I

V

Y

S

F

L

V

L

R

R

D

N

Q

S

I

I

K

K

L

L

T

A

C

L

K

K

H

Q

L

L

R

K

R

N

W

W

M

M

Q

A

P

P

E

E

K

K

Q

A

S

K

I

T

S

S

L

L

M

T

Q

T

Q

F

P

P

G

A

Q

S

A

A

Y

E

R

I

Q

V

A

S

E

E

P

P

L

L

G

G

V

V

L

L

I

V

I

I

G

S

P

A

W

W

N

N

Y

Y

P

P

F

F

Q

L

L

L

L

S

I

I

T

D

P

P

L

V

I

I

G

G

A

A

I

I

A

S

A

A

G

G

N

N

C

A

A

V

V

V

L

L

K

K

P

P

S

S

E
|
E

L

L

A

A

P

P

A

A

T

S

S

S

S

A

L

L

I

L

Q

T

R

K

L

L

I

L

S

E

D

Q

R

Y

F

L

D

D

P

P

D

S

Y

A

I

V

R

R

V

V

L

V

E

E

G

G

D

A

A
x
V

S

T

V

E

S

T

Q

S

A

A

L

L

I

L

T

E

Q

Q

P

K

F

W

D

D

H

K

I

I

F

F

F

Y

T

T

G

G

G

S

T

S

A

K

I
|
I

G

G

R

R

K

V

V

I

M

M

A

A

E

K

N

H

L

L

T

T

P

P

V

V

T

V

L

L

E

E

L

L

G

G

K

K

S

S

P

P

C

V

I

V

V

V

D

D

T

S

D

D

I

T

D

D

L

L

D

K

V

V

A

T

A

V

R

R

I

I

A

I

W

V

G

G

K

K

F

W

-

G

F
x
C

N

N

A

N

G

G

Q

Q

T

A

C
|
C

I

V

A

S

P

P

D

D

Y

Y

L

I

L

L

V

T

Q

T

R

K

T

E

V

Y

A

A

E

P

P

K

F

L

I

I

E

D

A

A

L

M

I

K

D

L

N

E

I

L

Q

E

Q

K

F

F

Y

Y

G

G

E

K

D

N

P

P

Q

I

Q

E

S

S

A

K

D

D

Y

M

A

S

R

R

I

I

V

V

S

N

D

S

R

N

H

H

W

F

Q

D

R

R

L

L

N

S

S

K

L

L

-

D

-

E

-

K

-

E

V

V

D

S

G

D

T

K

I

I

R

V

H

Y

G

G

Q

E

V

K

D

D

R

R

S

E

D

N

R

L

Y

K

I

I

A

A

P

P

T

T

L

I

I

L

T

L

D

D

V

V

N

P

W

L

R

D

D

S

P

L

I

I

L

M

Q

S

E

E

I

I

F

F

G

G

P

P

L

L

P

P

I

I

L

L

I

T

Y

L

D

N

Q

N

L

L

D

E

E

E

A

S

I

F

A

D

Q

V

I

I

R

R

A

S

Q

R

P

P

K

K

P

P

L

L

A

A

L

A

Y

Y

L

L

F

F

S

T

R

H

D

N

R

K

Q

K

V

L

Q

K

E

E

R

R

V

F

L

A

A

A

E

T

T

V

S

S

A

A

G

G

S

G

V

I

C

V

L

V

N

N

D

D

T

I

I

A

L

V

Q

H

V

L

G

A

V

L

P

H

D

T

A

L

A

P

F

F

G

G

V

V

G

G

P

E

S

S

G

G

M

M

G

G

G

A

Y

Y

H

H

G

G

K

K

A

F

S

S

F

F

E

D

T

A

F

F

S

S

H

H

Y

K

K

K

L

A

V

V

L

L

K

Y

R

R

P

S

F

L

W

F

L

G

D

D

L

S

A

A

L

V

R

R

Y

Y

P

P

Y

Y

G

S

-

R

D

G

K

K

I

L

N

R

L

L

F

L

R

K

K

A

L

L

C45 (≠ V42) mutation to S: Decreased solubility, loss of dimerization and strongly decreased activity.
E149 (= E146) mutation to D: Small effect on NAD(+) interaction, but 40% loss of efficiency. Ability to use NADP(+). 70% loss of efficiency with NAD(+); when associated with V-200. Impaired affinity for both cofactors and decreased catalytic efficiency; when associated with G-200.; mutation to N: Ability to use NADP(+) and 33% decreased efficiency with NAD(+). 70% loss of efficiency with NAD(+); when associated with V-200. Impaired affinity for both cofactors and decreased catalytic efficiency; when associated with G-200.; mutation to Q: Loss of specificity for NAD(+) and loss of 25% efficiency. 15% efficiency with NAD(+); when associated with V-200. Impaired affinity for both cofactors and decreased catalytic efficiency; when associated with G-200.; mutation to T: Loss of specificity and increased NADP(+) binding. Decreased catalytic efficiency. Loss of cofactor specificity and same lower efficiency with both; when associated with V-200. Impaired affinity for both cofactors and decreased catalytic efficiency; when associated with G-200. Changed coenzyme preference from NAD(+) to NADP(+), but no effect on the catalytic efficiency; when associated with R-178 and V-200.
V178 (≠ A175) mutation to R: Changed coenzyme preference from NAD(+) to NADP(+), but no effect on the catalytic efficiency; when associated with T-149 and V-200.
I200 (= I197) mutation to G: Changed coenzyme preference from NAD(+) to NADP(+), but impaired affinities for both cofactors. No effect on the interaction with the substrate. Impaired affinities for both cofactors and decreased catalytic efficiencies; when associated with D-149, Q-149, N-149 or T-149.; mutation to V: Also able to use NADP(+) as coenzyme, but no effect on the interaction with the substrate. 15% efficiency with NAD(+); when associated with Q-149. 70% loss of efficiency with NAD(+); when associated with D-149 or N-149. Loss of cofactor specificity and same lower efficiency with both; when associated with T-149. Changed coenzyme preference from NAD(+) to NADP(+), but no effect on the catalytic efficiency; when associated with T-149 and R-178.
C247 (≠ F243) mutation to S: No effect on solubility, but 10% loss of activity.
C253 (= C249) mutation to S: No effect on solubility, but loss of activity.

Q80VQ0 Aldehyde dehydrogenase family 3 member B1; Aldehyde dehydrogenase 7; EC 1.2.1.28; EC 1.2.1.5; EC 1.2.1.7 from Mus musculus (Mouse) (see paper)
47% identity, 96% coverage: 11:452/459 of query aligns to 6:449/468 of Q80VQ0

query
sites
Q80VQ0

E

D

T

K

L

L

A

Q

L

L

Q

R

A

E

T

A

F

F

D

K

R

E

G

G

D

R

T

T

R

R

T

S

L

A

A

E

F

F

R

R

L

A

A

A

R

Q

L

L

Q

Q

D

G

L

L

A

S

K

H

L

F

V

L

A

R

D

D

N

N

E

K

A

Q

E

Q

L

L

L

Q

Q

E

A

A

L

L

A

A

S

Q

D

D

L

L

R

H

K

K

P

S

A

A

L

F

E

E

A

A

Y

E

A

V

S

S

E

E

I

I

Y

A

F

I

V

S

R

Q

D

A

Q

E

I

V

K

D

L

L

T

A

C

L

K

R

H

N

L

L

R

R

R

S

W

W

M

M

Q

K

P

D

E

E

K

K

Q

V

S

S

I

K

S

N

L

L

M

A

Q

T

Q

Q

P

L

G

D

Q

S

A

A

Y

F

R

I

Q

R

A

K

E

E

P

P

L

F

G

G

V

L

V

V

L

L

I

I

G

V

P

P

W

W

N

N

Y

Y

P

P

F

I

Q

N

L

L

L

T

I

L

T

V

P

P

L

L

I

V

G

G

A

A

I

I

A

A

G

G

N

N

C

C

A

V

V

V

L

L

K

K

P

P

S

S

E

E

L

I

A

S

P

K

A

A

T

T

S

E

S

K

L

I

I

L

Q

A

R

E

L

V

I

L

S

P

D

R

R

Y

F

L

D

D

P

Q

D

S

Y

C

I

F

R

T

V

V

L

V

E

L

G

G

D

G

A

R

S

Q

V

E

S

T

Q

G

A

Q

L

L

I

L

T

E

Q

H

P

K

F

F

D

D

H

Y

I

I

F

F

T

T

G

G

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Sites not aligning to the query:

462 modified: S-palmitoyl cysteine; C→S: Reduces palmitoylation.
462:463 CC→SS: Abolishes palmitoylation.
463 modified: S-palmitoyl cysteine; C→S: Reduces palmitoylation.

P51648 Aldehyde dehydrogenase family 3 member A2; Aldehyde dehydrogenase 10; Fatty aldehyde dehydrogenase; Microsomal aldehyde dehydrogenase; EC 1.2.1.3; EC 1.2.1.94 from Homo sapiens (Human) (see 5 papers)
46% identity, 94% coverage: 16:448/459 of query aligns to 8:442/485 of P51648

query
sites
P51648

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I45 (≠ L53) to F: in SLS; severe loss of activity
V64 (= V72) to D: in SLS; severe loss of activity; dbSNP:rs72547556
L106 (= L114) to R: in SLS; severe loss of activity; dbSNP:rs72547558
N112 (= N120) mutation to A: Loss of enzyme activity.
P114 (= P122) to L: in SLS; severe loss of activity; dbSNP:rs72547559
P121 (= P129) to L: in SLS; severe loss of activity; dbSNP:rs72547560
T184 (= T192) to M: in SLS; severe loss of activity; dbSNP:rs72547562; to R: in SLS; severe loss of activity
G185 (= G193) to A: in SLS; severe loss of activity; dbSNP:rs72547563
E207 (= E215) active site; mutation to Q: Loss of enzyme activity.
C214 (= C222) to Y: in SLS; 4% of activity; dbSNP:rs72547564
R228 (= R236) to C: in SLS; severe loss of activity; dbSNP:rs72547566
C237 (≠ A245) to Y: in SLS; severe loss of activity; dbSNP:rs72547567
C241 (= C249) active site; mutation to S: Loss of enzyme activity.
D245 (= D253) to N: in SLS; severe loss of activity; dbSNP:rs72547568
K266 (≠ Q274) to N: in SLS; mild reduction of activity; the underlying nucleotide substitution affects transcript stability; dbSNP:rs72547569
Y279 (= Y287) to N: in SLS; severe loss of activity; dbSNP:rs72547570
AP 314:315 (= AP 322:323) natural variant: AP -> GAKSTVGA (in SLS; 8% of activity)
P315 (= P323) to S: in SLS; common mutation in Europeans; severe loss of enzymatic activity; dbSNP:rs72547571
E331 (= E339) mutation to Q: Loss of enzyme activity.
S365 (= S373) to L: in SLS; severe loss of activity; dbSNP:rs72547573
Y410 (= Y418) mutation to F: Decreased enzyme activity with dodecanal and hexadecanal. No effect on enzyme activity with octanal.
H411 (= H419) to Y: in SLS; severe loss of activity
S415 (= S423) to N: in SLS; severe loss of activity
F419 (= F427) to S: in SLS; severe loss of activity; dbSNP:rs72547576
R423 (≠ K431) to H: in SLS; severe loss of activity; dbSNP:rs768290318

Sites not aligning to the query:

445:485 mutation Missing: Decreased enzyme activity with dodecanal. Strongly decreased enzyme activity with hexadecanal. No effect on enzyme activity with octanal.
447 K → E: in SLS; severe loss of activity; dbSNP:rs67939114

E9Q3E1 Aldehyde dehydrogenase family 3 member B2; Aldehyde dehydrogenase 8; EC 1.2.1.3 from Mus musculus (Mouse) (see paper)
46% identity, 96% coverage: 12:450/459 of query aligns to 20:460/479 of E9Q3E1

query
sites
E9Q3E1

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