SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Synpcc7942_0489 Synpcc7942_0489 aldehyde dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P30838 Aldehyde dehydrogenase, dimeric NADP-preferring; ALDHIII; Aldehyde dehydrogenase 3; Aldehyde dehydrogenase family 3 member A1; EC 1.2.1.5 from Homo sapiens (Human) (see 4 papers)
49% identity, 96% coverage: 10:448/459 of query aligns to 5:445/453 of P30838

query
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P30838

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S134 (≠ C139) to A: in dbSNP:rs887241
E210 (= E215) active site
C244 (= C249) active site; mutation to S: Abolishes activity.
P329 (= P334) to A: in allele ALDH3A1*2; dbSNP:rs2228100

4l2oA Crystal structure of human aldh3a1 with its selective inhibitor 1-(4- fluorophenyl)sulfonyl-2-methylbenzimidazole
49% identity, 96% coverage: 10:448/459 of query aligns to 4:444/446 of 4l2oA

query
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4l2oA

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active site: N114 (= N120), K137 (= K143), E209 (= E215), C243 (= C249), E333 (= E339), Y412 (= Y418)
binding 1-[(4-fluorophenyl)sulfonyl]-2-methyl-1H-benzimidazole: E61 (≠ S67), Y65 (≠ F71), Y115 (= Y121), N118 (≠ Q124), L119 (= L125), M237 (≠ F243), C243 (= C249), I391 (= I397), I394 (≠ V400), T395 (≠ G401), F401 (= F407), H413 (= H419)
binding nicotinamide-adenine-dinucleotide: T112 (≠ P118), W113 (= W119), N114 (= N120), L119 (= L125), E140 (= E146), V169 (≠ A175), T186 (= T192), G187 (= G193), S188 (≠ G194), V191 (≠ I197), E209 (= E215), L210 (= L216), G211 (= G217), C243 (= C249), H289 (= H295), E333 (= E339), F335 (= F341), F401 (= F407)

4h80A Crystal structure of human aldh3a1 with its isozyme selective inhibitor - n-[4-(4-methylsulfonyl-2-nitroanilino)phenyl]acetamide
49% identity, 96% coverage: 10:448/459 of query aligns to 4:444/446 of 4h80A

query
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4h80A

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N

R

D

Q

K

V

V

Q

I

E

K

R

K

V

M

L

I

A

A

E

E

T

T

S

S

A

S

G

G

S

G

V

V

C

A

L

A

N

N

D

D

T

V

I

I

L

V

Q

H

V
x
I

G
x
T

V

L

P

H

D

S

A

L

A

P

F
|
F

G

G

V

V

G

G

P

N

S

S

G

G

M

M

G

G

G

S

Y
|
Y

H

H

G

G

K

K

A

K

S

S

F

F

E

E

T

T

F

F

S

S

H

H

Y

R

K

R

L

S

V

C

L

L

K

V

R

R

P

P

F

L

W

M

L

N

D

D

-

E

-

G

L

L

A

K

L

V

R

R

Y

Y

P

P

active site: N114 (= N120), K137 (= K143), E209 (= E215), C243 (= C249), E333 (= E339), Y412 (= Y418)
binding N-(4-{[4-(methylsulfonyl)-2-nitrophenyl]amino}phenyl)acetamide: E61 (≠ S67), Y65 (≠ F71), Y115 (= Y121), N118 (≠ Q124), W233 (= W239), T242 (= T248), C243 (= C249), V244 (≠ I250), I394 (≠ V400), T395 (≠ G401), F401 (= F407)

3szbA Crystal structure of human aldh3a1 modified with the beta-elimination product of aldi-1; 1-phenyl- 2-propen-1-one (see paper)
49% identity, 96% coverage: 10:448/459 of query aligns to 4:444/447 of 3szbA

query
sites
3szbA

A

S

E

E

T

A

L

V

A

K

A

R

L

A

Q

R

A

A

T

A

F

F

D

S

R

S

G

G

D

R

T

T

R

R

T

P

L

L

A

Q

F

F

R

R

L

I

A

Q

R

Q

L

L

Q

E

D

A

L

L

A

Q

K

R

L

L

V

I

A

Q

D

E

N

Q

E

E

A

Q

E

E

L

L

L

V

Q

G

A

A

L

L

A

A

S

A

D

D

L

L

R

H

K

K

P

N

A

E

L

W

E

N

A

A

Y

Y

A

Y

S

E

E

E

I

V

Y

V

F

Y

V

V

R

L

D

E

Q

E

I

I

K

E

L

Y

T

M

C

I

K

Q

H

K

L

L

R

P

R

E

W

W

M

A

Q

A

P

D

E

E

K

P

Q

V

S

E

I

K

S

T

L

P

M

Q

Q

T

Q

Q

P

Q

G

D

Q

E

A

L

Y

Y

R

I

Q

H

A

S

E

E

P

P

L

L

G

G

V

V

L

L

I

V

I

I

G

G

P

T

W

W

N
|
N

Y
|
Y

P

P

F

F

Q

N

L
|
L

L

T

I

I

T

Q

P

P

L

M

I

V

G

G

A

A

I

I

A

A

G

G

N

N

C

A

A

V

V

V

L

L

K
|
K

P

P

S

S

E

E

L

L

A

S

P

E

A

N

T

M

S

A

S

S

L

L

I

L

Q

A

R

T

L

I

I

I

S

P

D

Q

R

Y

F

L

D

D

P

K

D

D

Y

L

I

Y

R

P

V

V

L

I

E

N

G

G

D

G

A

V

S

P

V

E

S

T

Q

T

A

E

L

L

I

L

T

K

Q

E

P

R

F

F

D

D

H

H

I

I

F

L

F

Y

T

T

G

G

G

S

T

T

A

G

I

V

G

G

R

K

K

I

V

I

M

M

A

T

A

A

E

K

N

H

L

L

T

T

P

P

V

V

T

T

L

L

E
|
E

L

L

G

G

K

K

S

S

P

P

C

C

I

Y

V

V

D

D

T

K

D

N

I

C

D

D

L

L

D

D

V

V

A

A

A

C

R

R

I

I

A

A

W

W

G

G

K

K

F

F

F

M

N

N

A

S

G

G

Q

Q

T

T

C
|
C

I

V

A

A

P

P

D

D

Y

Y

L

I

L

L

V

C

Q

D

R

P

T

S

V

I

A

Q

E

N

P

Q

F

I

I

V

E

E

A

K

L

L

I

K

D

K

N

S

I

L

Q

K

Q

E

F

F

Y

Y

G

G

E

E

D

D

P

A

Q

K

S

S

A

R

D

D

Y

Y

A

G

R

R

I

I

V

I

S

S

D

A

R

R

H

H

W

F

Q

Q

R

R

L

V

N

M

S

G

L

L

L

I

V

E

D

G

G

Q

T

K

I

V

R

A

H

Y

G

G

Q

T

V

G

D

D

R

A

S

A

D

T

R

R

Y

Y

I

I

A

A

P

P

T

T

L

I

I

L

T

T

D

D

V

V

N

D

W

P

R

Q

D

S

P

P

I

V

L

M

Q

Q

E

E

E
|
E

I

I

F

F

G

G

P

P

L

V

L

L

P

P

I

I

L

V

I

C

Y

V

D

R

Q

S

L

L

D

E

E

E

A

A

I

I

A

Q

Q

F

I

I

R

N

A

Q

Q

R

P

E

K

K

P

P

L

L

A

A

L

L

Y

Y

L

M

F

F

S

S

R

S

D

N

R

D

Q

K

V

V

Q

I

E

K

R

K

V

M

L

I

A

A

E

E

T

T

S

S

A

S

G

G

S

G

V

V

C

A

L

A

N

N

D

D

T

V

I

I

L

V

Q

H

V
x
I

G

T

V

L

P

H

D

S

A

L

A

P

F

F

G

G

V

V

G

G

P

N

S

S

G

G

M

M

G

G

G

S

Y
|
Y

H

H

G

G

K

K

A

K

S

S

F

F

E

E

T

T

F

F

S

S

H

H

Y

R

K

R

L

S

V

C

L

L

K

V

R

R

P

P

F

L

W

M

L

N

D

D

-

E

-

G

L

L

A

K

L

V

R

R

Y

Y

P

P

active site: N114 (= N120), K137 (= K143), E209 (= E215), C243 (= C249), E333 (= E339), Y412 (= Y418)
binding 1-phenylpropan-1-one: Y115 (= Y121), L119 (= L125), C243 (= C249), I394 (≠ V400)

8bb8A Crystal structure of human aldehyde dehydrogenase aldh3a1 in complex with octanal (see paper)
49% identity, 96% coverage: 10:448/459 of query aligns to 4:444/447 of 8bb8A

query
sites
8bb8A

A

S

E

E

T

A

L

V

A

K

A

R

L

A

Q

R

A

A

T

A

F

F

D

S

R

S

G

G

D

R

T

T

R

R

T

P

L

L

A

Q

F

F

R

R

L

I

A

Q

R

Q

L

L

Q

E

D

A

L

L

A

Q

K

R

L

L

V

I

A

Q

D

E

N

Q

E

E

A

Q

E

E

L

L

L

V

Q

G

A

A

L

L

A

A

S

A

D

D

L

L

R

H

K

K

P

N

A

E

L

W

E

N

A

A

Y

Y

A

Y

S

E

E

E

I

V

Y

V

F
x
Y

V

V

R

L

D

E

Q

E

I

I

K

E

L

Y

T

M

C

I

K

Q

H

K

L

L

R

P

R

E

W

W

M

A

Q

A

P

D

E

E

K

P

Q

V

S

E

I

K

S

T

L

P

M

Q

Q

T

Q

Q

P

Q

G

D

Q

E

A

L

Y

Y

R

I

Q

H

A

S

E

E

P

P

L

L

G

G

V

V

L

L

I

V

I

I

G

G

P

T

W

W

N

N

Y
|
Y

P

P

F

F

Q
x
N

L

L

L

T

I

I

T

Q

P

P

L

M

I

V

G

G

A

A

I

I

A

A

G

G

N

N

C

S

A

V

V

V

L

L

K

K

P

P

S

S

E

E

L

L

A

S

P

E

A

N

T

M

S

A

S

S

L

L

I

L

Q

A

R

T

L

I

I

I

S

P

D

Q

R

Y

F

L

D

D

P

K

D

D

Y

L

I

Y

R

P

V

V

L

I

E

N

G

G

D

G

A

V

S

P

V

E

S

T

Q

T

A

E

L

L

I

L

T

K

Q

E

P

R

F

F

D

D

H

H

I

I

F

L

F

Y

T

T

G

G

G

S

T

T

A

G

I

V

G

G

R

K

K

I

V

I

M

M

A

T

A

A

E

K

N

H

L

L

T

T

P

P

V

V

T

T

L

L

E

E

L

L

G

G

K

K

S

S

P

P

C

C

I

Y

V

V

D

D

T

K

D

N

I

C

D

D

L

L

D

D

V

V

A

A

A

C

R

R

I

I

A

A

W

W

G

G

K

K

F

F

F

M

N

N

A

S

G

G

Q

Q

T

T

C
|
C

I

V

A

A

P

P

D

D

Y

Y

L

I

L

L

V

C

Q

D

R

P

T

S

V

I

A

Q

E

N

P

Q

F

I

I

V

E

E

A

K

L

L

I

K

D

K

N

S

I

L

Q

K

Q

E

F

F

Y

Y

G

G

E

E

D

D

P

A

Q

K

S

S

A

R

D

D

Y

Y

A

G

R

R

I

I

V

I

S

S

D

A

R

R

H

H

W

F

Q

Q

R

R

L

V

N

M

S

G

L

L

L

I

V

E

D

G

G

Q

T

K

I

V

R

A

H

Y

G

G

Q

T

V

G

D

D

R

A

S

A

D

T

R

R

Y

Y

I

I

A

A

P

P

T

T

L

I

I

L

T

T

D

D

V

V

N

D

W

P

R

Q

D

S

P

P

I

V

L

M

Q

Q

E

E

I

I

F

F

G

G

P

P

L

V

L

L

P

P

I

I

L

V

I

C

Y

V

D

R

Q

S

L

L

D

E

E

E

A

A

I

I

A

Q

Q

F

I

I

R

N

A

Q

Q

R

P

E

K

K

P

P

L

L

A

A

L

L

Y

Y

L

M

F

F

S

S

R

S

D

N

R

D

Q

K

V

V

Q

I

E

K

R

K

V

M

L

I

A

A

E

E

T

T

S

S

A

S

G

G

S

G

V

V

C

A

L

A

N

N

D

D

T

V

I

I

L

V

Q

H

V
x
I

G
x
T

V

L

P

H

D

S

A

L

A

P

F

F

G

G

V

V

G

G

P

N

S

S

G

G

M

M

G

G

G

S

Y

Y

H
|
H

G

G

K

K

A

K

S

S

F

F

E

E

T

T

F

F

S

S

H

H

Y

R

K

R

L

S

V

C

L

L

K

V

R

R

P

P

F

L

W

M

L

N

D

D

-

E

-

G

L

L

A

K

L

V

R

R

Y

Y

P

P

binding octanal: Y65 (≠ F71), Y115 (= Y121), N118 (≠ Q124), C243 (= C249), I394 (≠ V400), T395 (≠ G401), H413 (= H419)

4l1oB Crystal structure of human aldh3a1 with inhibitor 1-{[4-(1,3- benzodioxol-5-ylmethyl)piperazin-1-yl]methyl}-1h-indole-2,3-dione
49% identity, 96% coverage: 10:448/459 of query aligns to 4:444/452 of 4l1oB

query
sites
4l1oB

A

S

E

E

T

A

L

V

A

K

A

R

L

A

Q

R

A

A

T

A

F

F

D

S

R

S

G

G

D

R

T

T

R

R

T

P

L

L

A

Q

F

F

R

R

L

I

A

Q

R

Q

L

L

Q

E

D

A

L

L

A

Q

K

R

L

L

V

I

A

Q

D

E

N

Q

E

E

A

Q

E

E

L

L

L

V

Q

G

A

A

L

L

A

A

S

A

D

D

L

L

R

H

K

K

P

N

A

E

L

W

E

N

A

A

Y

Y

A

Y

S

E

E

E

I

V

Y

V

F

Y

V

V

R

L

D

E

Q

E

I

I

K

E

L

Y

T

M

C

I

K

Q

H

K

L

L

R

P

R

E

W

W

M

A

Q

A

P

D

E

E

K

P

Q

V

S

E

I

K

S

T

L

P

M

Q

Q

T

Q

Q

P

Q

G

D

Q

E

A

L

Y

Y

R

I

Q

H

A

S

E

E

P

P

L

L

G

G

V

V

L

L

I

V

I

I

G

G

P

T

W

W

N
|
N

Y
|
Y

P

P

F

F

Q
x
N

L
|
L

L

T

I

I

T

Q

P

P

L

M

I

V

G

G

A

A

I

I

A

A

G

G

N

N

C

A

A

V

V

V

L

L

K
|
K

P

P

S

S

E

E

L

L

A

S

P

E

A

N

T

M

S

A

S

S

L

L

I

L

Q

A

R

T

L

I

I

I

S

P

D

Q

R

Y

F

L

D

D

P

K

D

D

Y

L

I

Y

R

P

V

V

L

I

E

N

G

G

D

G

A

V

S

P

V

E

S

T

Q

T

A

E

L

L

I

L

T

K

Q

E

P

R

F

F

D

D

H

H

I

I

F

L

F

Y

T

T

G

G

G

S

T

T

A

G

I

V

G

G

R

K

K

I

V

I

M

M

A

T

A

A

E

K

N

H

L

L

T

T

P

P

V

V

T

T

L

L

E
|
E

L

L

G

G

K

K

S

S

P

P

C

C

I

Y

V

V

D

D

T

K

D

N

I

C

D

D

L

L

D

D

V

V

A

A

A

C

R

R

I

I

A

A

W

W

G

G

K

K

F

F

F

M

N

N

A

S

G

G

Q

Q

T
|
T

C
|
C

I

V

A

A

P

P

D

D

Y

Y

L

I

L

L

V

C

Q

D

R

P

T

S

V

I

A

Q

E

N

P

Q

F

I

I

V

E

E

A

K

L

L

I

K

D

K

N

S

I

L

Q

K

Q

E

F

F

Y

Y

G

G

E

E

D

D

P

A

Q

K

S

S

A

R

D

D

Y

Y

A

G

R

R

I

I

V

I

S

S

D

A

R

R

H

H

W

F

Q

Q

R

R

L

V

N

M

S

G

L

L

L

I

V

E

D

G

G

Q

T

K

I

V

R

A

H

Y

G

G

Q

T

V

G

D

D

R

A

S

A

D

T

R

R

Y

Y

I

I

A

A

P

P

T

T

L

I

I

L

T

T

D

D

V

V

N

D

W

P

R

Q

D

S

P

P

I

V

L

M

Q

Q

E

E

E
|
E

I

I

F

F

G

G

P

P

L

V

L

L

P

P

I

I

L

V

I

C

Y

V

D

R

Q

S

L

L

D

E

E

E

A

A

I

I

A

Q

Q

F

I

I

R

N

A

Q

Q

R

P

E

K

K

P

P

L

L

A

A

L

L

Y

Y

L

M

F

F

S

S

R

S

D

N

R

D

Q

K

V

V

Q

I

E

K

R

K

V

M

L

I

A

A

E

E

T

T

S

S

A

S

G

G

S

G

V

V

C

A

L

A

N

N

D

D

T

V

I
|
I

L

V

Q

H

V
x
I

G

T

V

L

P

H

D

S

A

L

A

P

F
|
F

G

G

V

V

G

G

P

N

S

S

G

G

M

M

G

G

G

S

Y
|
Y

H
|
H

G

G

K

K

A

K

S

S

F

F

E

E

T

T

F

F

S

S

H

H

Y

R

K

R

L

S

V

C

L

L

K

V

R

R

P

P

F

L

W

M

L

N

D

D

-

E

-

G

L

L

A

K

L

V

R

R

Y

Y

P

P

active site: N114 (= N120), K137 (= K143), E209 (= E215), C243 (= C249), E333 (= E339), Y412 (= Y418)
binding (3S)-1-{[4-(1,3-benzodioxol-5-ylmethyl)piperazin-1-yl]methyl}-3-hydroxy-1,3-dihydro-2H-indol-2-one: Y115 (= Y121), N118 (≠ Q124), L119 (= L125), E209 (= E215), T242 (= T248), C243 (= C249), I391 (= I397), I394 (≠ V400), F401 (= F407), H413 (= H419)

1ad3A Class 3 aldehyde dehydrogenase complex with nicotinamide-adenine- dinucleotide (see paper)
48% identity, 96% coverage: 10:448/459 of query aligns to 3:443/446 of 1ad3A

query
sites
1ad3A

A

S

E

D

T

T

L

V

A

K

A

R

L

A

Q

R

A

E

T

A

F

F

D

N

R

S

G

G

D

K

T

T

R

R

T

S

L

L

A

Q

F

F

R

R

L

I

A

Q

R

Q

L

L

Q

E

D

A

L

L

A

Q

K

R

L

M

V

I

A

N

D

E

N

N

E

L

A

K

E

S

L

I

L

S

Q

G

A

A

L

L

A

A

S

S

D

D

L

L

R

G

K

K

P

N

A

E

L

W

E

T

A

S

Y

Y

A

Y

S

E

E

E

I

V

Y

A

F

H

V

V

R

L

D

E

Q

E

I

L

K

D

L

T

T

T

C

I

K

K

H

E

L

L

R

P

R

D

W

W

M

A

Q

E

P

D

E

E

K

P

Q

V

S

A

I

K

S

T

L

R

M

Q

Q

T

Q

Q

P

Q

G

D

Q

D

A

L

Y

Y

R

I

Q

H

A

S

E

E

P

P

L

L

G

G

V

V

L

L

I

V

I

I

G

G

P
x
A

W
|
W

N
|
N

Y

Y

P

P

F

F

Q

N

L

L

L

T

I

I

T

Q

P

P

L

M

I

V

G

G

A

A

I

V

A

A

G

G

N

N

C

A

A

V

V

I

L

L

K
|
K

P

P

S

S

E
|
E

L
x
V

A

S

P

G

A

H

T

M

S

A

S

D

L

L

I

L

Q

A

R

T

L

L

I

I

S

P

D

Q

R

Y

F

M

D

D

P

Q

D

N

Y

L

I

Y

R

L

V

V

L

V

E

K

G

G

D

G

A
x
V

S

P

V

E

S

T

Q

T

A

E

L

L

I

L

T

K

Q

E

P

R

F

F

D

D

H

H

I

I

F

M

F

Y

T

T

G
|
G

G

S

T

T

A

A

I
x
V

G

G

R

K

K

I

V

V

M

M

A

A

E

K

N

H

L

L

T

T

P

P

V

V

T

T

L

L

E
|
E

L

L

G

G

K

K

S

S

P

P

C

C

I

Y

V

V

D

D

T

K

D

D

I

C

D

D

L

L

D

D

V

V

A

A

A

C

R

R

I

I

A

A

W

W

G

G

K

K

F

F

F

M

N

N

A

S

G

G

Q

Q

T

T

C
|
C

I

V

A

A

P

P

D

D

Y

Y

L

I

L

L

V

C

Q

D

R

P

T

S

V

I

A

Q

E

N

P

Q

F

I

I

V

E

E

A

K

L

L

I

K

D

K

N

S

I

L

Q

K

Q

D

F

F

Y

Y

G

G

E

E

D

D

P

A

Q

K

Q

Q

S

S

A

R

D

D

Y

Y

A

G

R

R

I

I

V

I

S

N

D

D

R

R

H
|
H

W

F

Q

Q

R
|
R

L

V

N

K

S

G

L

L

L

I

V

D

D

N

G

Q

T

K

I

V

R

A

H

H

G

G

Q

T

V

W

D

D

R

Q

S

S

D

S

R

R

Y

Y

I

I

A

A

P

P

T

T

L

I

I

L

T

V

D

D

V

V

N

D

W

P

R

Q

D

S

P

P

I

V

L

M

Q

Q

E

E

E
|
E

I

I

F
|
F

G

G

P

P

L

V

L

M

P

P

I

I

L

V

I

C

Y

V

D

R

Q

S

L

L

D

E

E

E

A

A

I

I

A

Q

Q

F

I

I

R

N

A

Q

Q

R

P

E

K

K

P

P

L

L

A

A

L

L

Y

Y

L

V

F

F

S

S

R

N

D

N

R

E

Q

K

V

V

Q

I

E

K

R

K

V

M

L

I

A

A

E

E

T

T

S

S

A

S

G

G

S

G

V

V

C

T

L

A

N

N

D

D

T

V

I

I

L

V

Q

H

V

I

G

T

V

V

P

P

D

T

A

L

A

P

F

F

G

G

V

V

G

G

P

N

S

S

G

G

M

M

G

G

G

A

Y
|
Y

H

H

G

G

K

K

A

K

S

S

F

F

E

E

T

T

F

F

S

S

H

H

Y

R

K

R

L

S

V

C

L

L

K

V

R

K

P

S

F

L

W

L

L

N

D

E

L

E

A

A

-

H

-

K

L

A

R

R

Y

Y

P

P

active site: N113 (= N120), K136 (= K143), E208 (= E215), C242 (= C249), E332 (= E339), Y411 (= Y418)
binding nicotinamide-adenine-dinucleotide: A111 (≠ P118), W112 (= W119), N113 (= N120), E139 (= E146), V140 (≠ L147), V168 (≠ A175), G186 (= G193), V190 (≠ I197), H288 (= H295), R291 (= R298), E332 (= E339), F334 (= F341)

Q70DU8 Aldehyde dehydrogenase family 3 member H1; AtALDH4; Ath-ALDH4; EC 1.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
48% identity, 98% coverage: 9:459/459 of query aligns to 12:468/484 of Q70DU8

query
sites
Q70DU8

A

A

A

S

E

N

T

L

L

V

A

T

A

E

L

L

Q

R

A

R

T

S

F

F

D

D

R

D

G

G

D

V

T

T

R

R

T

G

L

Y

A

E

F

W

R

R

L

V

A

T

R

Q

L

L

Q

K

D

K

L

L

A

M

K

I

L

I

V
x
C

A

D

D

N

N

H

E

E

A

P

E

E

L

I

L

V

Q

A

A

A

L

L

A

R

S

D

D

D

L

L

R

G

K

K

P

P

A

E

L

L

E

E

A

S

Y

S

A

V

S

Y

E

E

I

V

Y

S

F

L

V

L

R

R

D

N

Q

S

I

I

K

K

L

L

T

A

C

L

K

K

H

Q

L

L

R

K

R

N

W

W

M

M

Q

A

P

P

E

E

K

K

Q

A

S

K

I

T

S

S

L

L

M

T

Q

T

Q

F

P

P

G

A

Q

S

A

A

Y

E

R

I

Q

V

A

S

E

E

P

P

L

L

G

G

V

V

L

L

I

V

I

I

G

S

P

A

W

W

N

N

Y

Y

P

P

F

F

Q

L

L

L

L

S

I

I

T

D

P

P

L

V

I

I

G

G

A

A

I

I

A

S

A

A

G

G

N

N

C

A

A

V

V

V

L

L

K

K

P

P

S

S

E
|
E

L

L

A

A

P

P

A

A

T

S

S

S

S

A

L

L

I

L

Q

T

R

K

L

L

I

L

S

E

D

Q

R

Y

F

L

D

D

P

P

D

S

Y

A

I

V

R

R

V

V

L

V

E

E

G

G

D

A

A
x
V

S

T

V

E

S

T

Q

S

A

A

L

L

I

L

T

E

Q

Q

P

K

F

W

D

D

H

K

I

I

F

F

F

Y

T

T

G

G

G

S

T

S

A

K

I
|
I

G

G

R

R

K

V

V

I

M

M

A

A

E

K

N

H

L

L

T

T

P

P

V

V

T

V

L

L

E

E

L

L

G

G

K

K

S

S

P

P

C

V

I

V

V

V

D

D

T

S

D

D

I

T

D

D

L

L

D

K

V

V

A

T

A

V

R

R

I

I

A

I

W

V

G

G

K

K

F

W

-

G

F
x
C

N

N

A

N

G

G

Q

Q

T

A

C
|
C

I

V

A

S

P

P

D

D

Y

Y

L

I

L

L

V

T

Q

T

R

K

T

E

V

Y

A

A

E

P

P

K

F

L

I

I

E

D

A

A

L

M

I

K

D

L

N

E

I

L

Q

E

Q

K

F

F

Y

Y

G

G

E

K

D

N

P

P

Q

I

Q

E

S

S

A

K

D

D

Y

M

A

S

R

R

I

I

V

V

S

N

D

S

R

N

H

H

W

F

Q

D

R

R

L

L

N

S

S

K

L

L

-

D

-

E

-

K

-

E

V

V

D

S

G

D

T

K

I

I

R

V

H

Y

G

G

Q

E

V

K

D

D

R

R

S

E

D

N

R

L

Y

K

I

I

A

A

P

P

T

T

L

I

I

L

T

L

D

D

V

V

N

P

W

L

R

D

D

S

P

L

I

I

L

M

Q

S

E

E

I

I

F

F

G

G

P

P

L

L

P

P

I

I

L

L

I

T

Y

L

D

N

Q

N

L

L

D

E

E

E

A

S

I

F

A

D

Q

V

I

I

R

R

A

S

Q

R

P

P

K

K

P

P

L

L

A

A

L

A

Y

Y

L

L

F

F

S

T

R

H

D

N

R

K

Q

K

V

L

Q

K

E

E

R

R

V

F

L

A

A

A

E

T

T

V

S

S

A

A

G

G

S

G

V

I

C

V

L

V

N

N

D

D

T

I

I

A

L

V

Q

H

V

L

G

A

V

L

P

H

D

T

A

L

A

P

F

F

G

G

V

V

G

G

P

E

S

S

G

G

M

M

G

G

G

A

Y

Y

H

H

G

G

K

K

A

F

S

S

F

F

E

D

T

A

F

F

S

S

H

H

Y

K

K

K

L

A

V

V

L

L

K

Y

R

R

P

S

F

L

W

F

L

G

D

D

L

S

A

A

L

V

R

R

Y

Y

P

P

Y

Y

G

S

-

R

D

G

K

K

I

L

N

R

L

L

F

L

R

K

K

A

L

L

C45 (≠ V42) mutation to S: Decreased solubility, loss of dimerization and strongly decreased activity.
E149 (= E146) mutation to D: Small effect on NAD(+) interaction, but 40% loss of efficiency. Ability to use NADP(+). 70% loss of efficiency with NAD(+); when associated with V-200. Impaired affinity for both cofactors and decreased catalytic efficiency; when associated with G-200.; mutation to N: Ability to use NADP(+) and 33% decreased efficiency with NAD(+). 70% loss of efficiency with NAD(+); when associated with V-200. Impaired affinity for both cofactors and decreased catalytic efficiency; when associated with G-200.; mutation to Q: Loss of specificity for NAD(+) and loss of 25% efficiency. 15% efficiency with NAD(+); when associated with V-200. Impaired affinity for both cofactors and decreased catalytic efficiency; when associated with G-200.; mutation to T: Loss of specificity and increased NADP(+) binding. Decreased catalytic efficiency. Loss of cofactor specificity and same lower efficiency with both; when associated with V-200. Impaired affinity for both cofactors and decreased catalytic efficiency; when associated with G-200. Changed coenzyme preference from NAD(+) to NADP(+), but no effect on the catalytic efficiency; when associated with R-178 and V-200.
V178 (≠ A175) mutation to R: Changed coenzyme preference from NAD(+) to NADP(+), but no effect on the catalytic efficiency; when associated with T-149 and V-200.
I200 (= I197) mutation to G: Changed coenzyme preference from NAD(+) to NADP(+), but impaired affinities for both cofactors. No effect on the interaction with the substrate. Impaired affinities for both cofactors and decreased catalytic efficiencies; when associated with D-149, Q-149, N-149 or T-149.; mutation to V: Also able to use NADP(+) as coenzyme, but no effect on the interaction with the substrate. 15% efficiency with NAD(+); when associated with Q-149. 70% loss of efficiency with NAD(+); when associated with D-149 or N-149. Loss of cofactor specificity and same lower efficiency with both; when associated with T-149. Changed coenzyme preference from NAD(+) to NADP(+), but no effect on the catalytic efficiency; when associated with T-149 and R-178.
C247 (≠ F243) mutation to S: No effect on solubility, but 10% loss of activity.
C253 (= C249) mutation to S: No effect on solubility, but loss of activity.

Q80VQ0 Aldehyde dehydrogenase family 3 member B1; Aldehyde dehydrogenase 7; EC 1.2.1.28; EC 1.2.1.5; EC 1.2.1.7 from Mus musculus (Mouse) (see paper)
47% identity, 96% coverage: 11:452/459 of query aligns to 6:449/468 of Q80VQ0

query
sites
Q80VQ0

E

D

T

K

L

L

A

Q

L

L

Q

R

A

E

T

A

F

F

D

K

R

E

G

G

D

R

T

T

R

R

T

S

L

A

A

E

F

F

R

R

L

A

A

A

R

Q

L

L

Q

Q

D

G

L

L

A

S

K

H

L

F

V

L

A

R

D

D

N

N

E

K

A

Q

E

Q

L

L

L

Q

Q

E

A

A

L

L

A

A

S

Q

D

D

L

L

R

H

K

K

P

S

A

A

L

F

E

E

A

A

Y

E

A

V

S

S

E

E

I

I

Y

A

F

I

V

S

R

Q

D

A

Q

E

I

V

K

D

L

L

T

A

C

L

K

R

H

N

L

L

R

R

R

S

W

W

M

M

Q

K

P

D

E

E

K

K

Q

V

S

S

I

K

S

N

L

L

M

A

Q

T

Q

Q

P

L

G

D

Q

S

A

A

Y

F

R

I

Q

R

A

K

E

E

P

P

L

F

G

G

V

L

V

V

L

L

I

I

G

V

P

P

W

W

N

N

Y

Y

P

P

F

I

Q

N

L

L

L

T

I

L

T

V

P

P

L

L

I

V

G

G

A

A

I

I

A

A

G

G

N

N

C

C

A

V

V

V

L

L

K

K

P

P

S

S

E

E

L

I

A

S

P

K

A

A

T

T

S

E

S

K

L

I

I

L

Q

A

R

E

L

V

I

L

S

P

D

R

R

Y

F

L

D

D

P

Q

D

S

Y

C

I

F

R

T

V

V

L

V

E

L

G

G

D

G

A

R

S

Q

V

E

S

T

Q

G

A

Q

L

L

I

L

T

E

Q

H

P

K

F

F

D

D

H

Y

I

I

F

F

T

T

G

G

G

N

T

A

A

Y

I

V

G

G

R

K

K

I

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V

M

M

A

A

E

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N

H

L

L

T

T

P

P

V

I

T

T

L

L

E

E

L

L

G

G

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K

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N

P

P

C

C

I

Y

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D

D

T

D

D

N

I

C

D

D

L

P

D

Q

V

I

A

V

A

A

R

N

R

R

I

V

A

A

W

W

G

F

K

R

F

Y

F

F

N

N

A

A

G

G

Q

Q

T

T

C

C

I

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A

A

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P

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D

Y

Y

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M

A

Q

E

E

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I

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A

A

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I

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D

N

N

A

I

I

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Y

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G

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A

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Y

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A

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I

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F

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Q

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G

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C

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V

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S

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E

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R

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I

I

A

A

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T

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D

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Q

W

E

R

T

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E

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Q

E

E

I

I

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F

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G

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P

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I

L

L

P

P

I

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L

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I

T

Y

V

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R

Q

S

L

L

D

D

E

E

A

A

I

I

A

E

Q

F

I

M

R

N

A

R

Q

R

P

E

K

K

P

P

L

L

A

A

L

L

Y

Y

L

A

F

F

S

S

R

K

D

R

R

S

Q

Q

V

V

Q

I

E

K

R

Q

V

V

L

L

A

A

E

R

T

T

S

S

A

S

G

G

S

G

V

F

C

C

L

G

N

N

D

D

T

G

I

F

L

M

Q

H

V

M

G

T

V

L

P

S

D

S

A

L

A

P

F

F

G

G

V

V

G

G

P

T

S

S

G

G

M

M

G

G

G

R

Y

Y

H

H

G

G

K

K

A

F

S

S

F

F

E

D

T

T

F

F

S

S

H

N

Y

Q

K

R

L

A

V

C

L

L

K

L

R

R

P

S

F

P

W

G

L

M

D

E

L

K

A

I

-

N

-

D

L

L

R

R

Y

Y

P

P

Y

Y

G

S

D

S

K

R

Sites not aligning to the query:

462 modified: S-palmitoyl cysteine; C→S: Reduces palmitoylation.
462:463 CC→SS: Abolishes palmitoylation.
463 modified: S-palmitoyl cysteine; C→S: Reduces palmitoylation.

P51648 Aldehyde dehydrogenase family 3 member A2; Aldehyde dehydrogenase 10; Fatty aldehyde dehydrogenase; Microsomal aldehyde dehydrogenase; EC 1.2.1.3; EC 1.2.1.94 from Homo sapiens (Human) (see 5 papers)
46% identity, 94% coverage: 16:448/459 of query aligns to 8:442/485 of P51648

query
sites
P51648

L

V

Q

R

A

Q

T

A

F

F

D

L

R

S

G

G

D

R

T

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L

L

A

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L

Q

E

D

A

L

L

A

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V

A

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E

N

R

E

E

A

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E

D

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I

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A

A

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x
I

A

A

S

A

D

D

L

L

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C

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K

P

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A

E

L

F

E

N

A

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Y

Y

A

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S

Q

E

E

I

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Y

I

F

T

V
|
V

R

L

D

G

Q

E

I

I

K

D

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C

L

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E

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W

W

M

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P

A

E

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V

S

K

I

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S

N

L

V

M

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Q

M

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Q

E

A

A

Y

Y

R

I

Q

Q

A

P

E

Q

P

P

L

L

G

G

V

V

L
|
L

I

I

G

G

P

A

W

W

N
|
N

Y

Y

P
|
P

F

F

Q

V

L

L

L

T

I

I

T

Q

P
|
P

L

L

I

I

G

G

A

A

I

I

A

A

G

G

N

N

C

A

A

V

V

I

L

I

K

K

P

P

S

S

E

E

L

L

A

S

P

E

A

N

T

T

S

A

S

K

L

I

I

L

Q

A

R

K

L

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I

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Y

F

L

D

D

P

Q

D

D

Y

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Y

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I

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V

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G

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F

D

D

H

H

I

I

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F

F

Y

T
|
T

G
|
G

G

N

T

T

A

A

I

V

G

G

R

K

K

I

V

V

M

M

A

E

A

A

E

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H

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L

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P

V

V

T

T

L

L

E
|
E

L

L

G

G

K

K

S

S

P

P

C
|
C

I

Y

V

I

D

D

T

K

D

D

I

C

D

D

L

L

D

D

V

I

A

V

A

C

R

R

R
|
R

I

I

A

T

W

W

G

G

K

K

F

Y

F

M

N

N

A
x
C

G

G

Q

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T

T

C
|
C

I

I

A

A

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P

D
|
D

Y

Y

L

I

L

L

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C

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E

R

A

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L

A

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A

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I

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N

T

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x
K

Q

E

F

F

Y

Y

G

G

E

E

D

N

P

I

Q

K

Q

E

S

S

A

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D

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Y
|
Y

A

E

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R

I

I

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A

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D

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|
E

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S

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G

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N

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T

V

L

P

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D

S

A

F

A

P

F

F

G

G

V

V

G

G

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S

S

S

G

G

M

M

G

G

G

A

Y
|
Y

H
|
H

G

G

K

K

A

H

S
|
S

F

F

E

D

T

T

F
|
F

S

S

H

H

Y

Q

K
x
R

L

P

V

C

L

L

K

L

R

K

P

S

F

L

W

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E

L

G

A

A

-

N

-

K

L

L

R

R

Y

Y

P

P

I45 (≠ L53) to F: in SLS; severe loss of activity
V64 (= V72) to D: in SLS; severe loss of activity; dbSNP:rs72547556
L106 (= L114) to R: in SLS; severe loss of activity; dbSNP:rs72547558
N112 (= N120) mutation to A: Loss of enzyme activity.
P114 (= P122) to L: in SLS; severe loss of activity; dbSNP:rs72547559
P121 (= P129) to L: in SLS; severe loss of activity; dbSNP:rs72547560
T184 (= T192) to M: in SLS; severe loss of activity; dbSNP:rs72547562; to R: in SLS; severe loss of activity
G185 (= G193) to A: in SLS; severe loss of activity; dbSNP:rs72547563
E207 (= E215) active site; mutation to Q: Loss of enzyme activity.
C214 (= C222) to Y: in SLS; 4% of activity; dbSNP:rs72547564
R228 (= R236) to C: in SLS; severe loss of activity; dbSNP:rs72547566
C237 (≠ A245) to Y: in SLS; severe loss of activity; dbSNP:rs72547567
C241 (= C249) active site; mutation to S: Loss of enzyme activity.
D245 (= D253) to N: in SLS; severe loss of activity; dbSNP:rs72547568
K266 (≠ Q274) to N: in SLS; mild reduction of activity; the underlying nucleotide substitution affects transcript stability; dbSNP:rs72547569
Y279 (= Y287) to N: in SLS; severe loss of activity; dbSNP:rs72547570
AP 314:315 (= AP 322:323) natural variant: AP -> GAKSTVGA (in SLS; 8% of activity)
P315 (= P323) to S: in SLS; common mutation in Europeans; severe loss of enzymatic activity; dbSNP:rs72547571
E331 (= E339) mutation to Q: Loss of enzyme activity.
S365 (= S373) to L: in SLS; severe loss of activity; dbSNP:rs72547573
Y410 (= Y418) mutation to F: Decreased enzyme activity with dodecanal and hexadecanal. No effect on enzyme activity with octanal.
H411 (= H419) to Y: in SLS; severe loss of activity
S415 (= S423) to N: in SLS; severe loss of activity
F419 (= F427) to S: in SLS; severe loss of activity; dbSNP:rs72547576
R423 (≠ K431) to H: in SLS; severe loss of activity; dbSNP:rs768290318

Sites not aligning to the query:

445:485 mutation Missing: Decreased enzyme activity with dodecanal. Strongly decreased enzyme activity with hexadecanal. No effect on enzyme activity with octanal.
447 K → E: in SLS; severe loss of activity; dbSNP:rs67939114

E9Q3E1 Aldehyde dehydrogenase family 3 member B2; Aldehyde dehydrogenase 8; EC 1.2.1.3 from Mus musculus (Mouse) (see paper)
46% identity, 96% coverage: 12:450/459 of query aligns to 20:460/479 of E9Q3E1

query
sites
E9Q3E1

T

T

L

L

A

H

A

S

L

L

Q

R

A

E

T

A

F

F

D

N

R

A

G

G

D

R

T

T

R

R

T

P

L

T

A

E

F

F

R

R

L

T

A

A

R

Q

L

L

Q

R

D

S

L

L

A

G

K

R

L

F

V

L

A

Q

D

E

N

N

E

K

A

E

E

L

L

L

L

Q

Q

D

A

A

L

L

A

A

S

K

D

D

L

V

R

G

K

K

P

S

A

G

L

F

E

E

A

S

Y

D

A

M

S

S

E

E

I

I

Y

I

F

L

V

C

R

E

D

N

Q

E

I

V

K

D

L

L

T

A

C

L

K

K

H

N

L

L

R

Q

R

T

W

W

M

M

Q

K

P

D

E

E

K

P

Q

V

S

S

I

T

S

N

L

L

M

L

Q

T

Q

K

P

L

G

S

Q

S

A

A

Y

F

R

I

Q

R

A

K

E

E

P

P

L

F

G

G

V

L

V

V

L

L

I

I

G

A

P

P

W

W

N

N

Y

Y

P

P

F

V

Q

N

L

L

L

M

I

I

T

I

P

P

L

L

I

V

G

G

A

A

I

I

A

A

G

G

N

N

C

C

A

V

V

V

L

L

K

K

P

P

S

S

E

E

L

I

A

S

P

K

A

N

T

T

S

E

S

K

L

V

I

L

Q

A

R

E

L

L

I

L

S

P

D

Q

R

Y

F

L

D

D

P

Q

D

S

Y

C

I

F

R

A

V

V

L

M

E

L

G

G

D

G

A

P

S

E

V

E

S

T

Q

R

A

Q

L

L

I

L

T

E

Q

H

P

K

F

F

D

D

H

Y

I

I

F

F

T

T

G

G

G

S

T

P

A

R

I

V

G

G

R

K

K

I

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V

M

M

A

T

A

A

E

K

N

H

L

L

T

T

P

P

V

I

T

T

L

L

E

E

L

L

G

G

K

K

S

N

P

P

C

C

I

Y

V

V

D

D

T

D

D

N

I

C

D

D

L

P

D

Q

V

T

A

V

A

A

R

N

R

R

I

V

A

A

W

W

G

F

K

R

F

Y

F

F

N

N

A

A

G

G

Q

Q

T

T

C

C

I

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A

A

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P

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D

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Y

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A

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P

A

A

L

L

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Q

D

N

N

S

I

I

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F

F

Y

Y

G

G

E

D

D

N

P

P

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Q

Q

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S

S

A

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D

N

Y

L

A

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I

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I

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F

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K

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L

L

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L

L

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C

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A

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I

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Q

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S

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Y

I

I

A

A

P

P

T

T

L

V

I

L

T

V

D

D

V

V

N

Q

W

E

R

T

D

E

P

P

I

V

L

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Q

Q

E

E

I

I

F

F

G

G

P

P

L

I

L

L

P

P

I

L

L

V

I

T

Y

V

D

R

Q

S

L

L

D

D

E

E

A

A

I

I

A

E

Q

F

I

I

R

N

A

R

Q

R

P

E

K

K

P

P

L

L

A

A

L

L

Y

Y

L

A

F

F

S

S

R

N

D

N

R

N

Q

Q

V

V

Q

V

E

N

R

Q

V

M

L

L

A

E

E

R

T

T

S

S

A

S

G

G

S

G

V

F

C

G

L

G

N

N

D

D

T

G

I

F

L

L

Q

Y

V

L

G

T

V

L

P

P

D

A

A

L

A

P

F

L

G

G

V

V

G

G

P

N

S

S

G

G

M

M

G

G

G

R

Y

Y

H

H

G

G

K

K

A

F

S

S

F

F

E

D

T

T

F

F

S

S

H

H

Y

H

K

R

L

A

V

C

L

L

K

L

R

R

P

S

F

P

W

G

L

M

D

E

L

K

A

L

-

N

-

D

L

L

R

R

Y

Y

P

P

Y

Y

G

G

Sites not aligning to the query:

462 W→A: Reduces lipid droplet localization.
469 W→A: Reduces lipid droplet localization.
476 C→S: Reduces lipid droplet localization.

P43353 Aldehyde dehydrogenase family 3 member B1; Aldehyde dehydrogenase 7; EC 1.2.1.28; EC 1.2.1.5; EC 1.2.1.7 from Homo sapiens (Human) (see paper)
47% identity, 96% coverage: 10:448/459 of query aligns to 5:445/468 of P43353

query
sites
P43353

A

G

E

D

T

T

L

L

A

R

L

L

Q

R

A

E

T

A

F

F

D

H

R

A

G

G

D

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T

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P

L

A

A

E

F

F

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R

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A

A

A

R

Q

L

L

Q

Q

D

G

L

L

A

G

K

R

L

F

V

L

A

Q

D

E

N

N

E

K

A

Q

E

L

L

L

L

H

Q

D

A

A

L

L

A

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Q

D

D

L

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H

K

K

P

S

A

A

L

F

E

E

A

S

Y

E

A

V

S

S

E

E

I

V

Y

A

F

I

V

S

R

Q

D

G

Q

E

I

V

K

T

L

L

T

A

C

L

K

R

H

N

L

L

R

R

R

A

W

W

M

M

Q

K

P

D

E

E

K

R

Q

V

S

P

I

K

S

N

L

L

M

A

Q

T

Q

Q

P

L

G

D

Q

S

A

A

Y

F

R

I

Q

R

A

K

E

E

P

P

L

F

G

G

V

L

V

V

L

L

I

I

G

A

P

P

W

W

N

N

Y

Y

P

P

F

L

Q

N

L

L

L

T

I

L

T

V

P

P

L

L

I

V

G

G

A

A

I

L

A

A

G

G

N

N

C

C

A

V

V

V

L

L

K

K

P

P

S

S

E

E

L

I

A

S

P

K

A

N

T

V

S

E

S

K

L

I

I

L

Q

A

R

E

L

V

I

L

S

P

D

Q

R

Y

F

V

D

D

P

Q

D

S

Y

C

I

F

R

A

V

V

L

V

E

L

G

G

D

G

A

P

S

Q

V

E

S

T

Q

G

A

Q

L

L

I

L

T

E

Q

H

P

R

F

F

D

D

H

Y

I

I

F

F

T

T

G

G

G

S

T

P

A

R

I

V

G

G

R

K

K

I

V

V

M

M

A

T

A

A

E

K

N

H

L

L

T

T

P

P

V

V

T

T

L

L

E

E

L

L

G

G

K

K

S

N

P

P

C

C

I

Y

V

V

D

D

T

D

D

N

I

C

D

D

L

P

D

Q

V

T

A

V

A

A

R

N

R

R

I

V

A

A

W

W

G

F

K

R

F

Y

F

F

N

N

A

A

G

G

Q

Q

T

T

C

C

I

V

A

A

P

P

D

D

Y

Y

L

V

L

L

V

C

Q

S

R

P

T

E

V

M

A

Q

E

E

P

R

F

L

I

L

E

P

A

A

L

L

I

Q

D

S

N

T

I

I

Q

T

Q

R

F

F

Y

Y

G

G

E

D

D

D

P

P

Q

Q

Q

S

S

S

A

P

D

N

Y

L

A

G

R

R

I

I

V

I

S

N

D

Q

R

K

H

Q

W

F

Q

Q

R

R

L

L

N

R

S

A

L

L

V

G

D

C

G

G

T

R

I

V

R

A

H

I

G

G

Q

Q

V

S

D

D

R

E

S

S

D

D

R

R

Y

Y

I

I

A

A

P

P

T

T

L

V

I

L

T

V

D

D

V

V

N

Q

W

E

R

M

D

E

P

P

I

V

L

M

Q

Q

E

E

I

I

F

F

G

G

P

P

L

I

L

L

P

P

I

I

L

V

I

N

Y

V

D

Q

Q

S

L

L

D

D

E

E

A

A

I

I

A

E

Q

F

I

I

R

N

A

R

Q

R

P

E

K

K

P

P

L

L

A

A

L

L

Y

Y

L

A

F

F

S

S

R

N

D

S

R

S

Q

Q

V

V

Q

V

E

K

R

R

V

V

L

L

A

T

E

Q

T

T

S

S

A

S

G

G

S

G

V

F

C

C

L

G

N

N

D

D

T

G

I

F

L

M

Q

H

V

M

G

T

V

L

P

A

D

S

A

L

A

P

F

F

G

G

V

V

G

G

P

A

S

S

G

G

M

M

G

G

G

R

Y

Y

H

H

G

G

K

K

A

F

S

S

F

F

E

D

T

T

F

F

S

S

H

H

Y

H

K

R

L

A

V

C

L

L

K

L

R

R

P

S

F

P

W

G

L

M

D

E

L

K

-

L

-

N

A

A

L

L

R

R

Y

Y

P

P

Sites not aligning to the query:

463 modified: S-palmitoyl cysteine
465 modified: S-geranylgeranyl cysteine

J3QMK6 Aldehyde dehydrogenase family 3 member B3; EC 1.2.1.3 from Mus musculus (Mouse) (see paper)
46% identity, 96% coverage: 11:449/459 of query aligns to 19:459/479 of J3QMK6

query
sites
J3QMK6

E

E

T

K

L

L

A

Q

A

R

L

L

Q

K

A

E

T

A

F

F

D

N

R

T

G

G

D

K

T

T

R

K

T

T

L

A

A

K

F

F

R

R

L

A

A

E

R

Q

L

L

Q

Q

D

S

L

L

A

G

K

R

L

F

V

L

A

Q

D

D

N

N

E

S

A

K

E

Q

L

L

L

H

Q

D

A

A

L

L

A

D

S

G

D

D

L

L

R

G

K

K

P

S

A

G

L

F

E

E

A

S

Y

D

A

M

S

S

E

E

I

I

Y

I

F

L

V

C

R

E

D

N

Q

E

I

V

K

D

L

L

T

A

C

L

K

K

H

N

L

L

R

Q

R

T

W

W

M

M

Q

K

P

D

E

E

K

P

Q

V

S

S

I

T

S

N

L

L

M

L

Q

T

Q

K

P

L

G

S

Q

T

A

A

Y

F

R

I

Q

R

A

K

E

E

P

P

L

F

G

G

V

L

V

V

L

L

I

I

G

A

P

P

W

W

N

N

Y

Y

P

P

F

V

Q

N

L

L

L

M

I

I

T

I

P

P

L

L

I

V

G

G

A

A

I

I

A

A

G

G

N

N

C

C

A

V

V

V

L

L

K

K

P

P

S

S

E

E

L

I

A

S

P

K

A

N

T

T

S

E

S

K

L

V

I

L

Q

A

R

E

L

L

I

L

S

P

D

Q

R

Y

F

L

D

D

P

Q

D

S

Y

C

I

F

R

A

V

V

L

M

E

L

G

G

D

G

A

P

S

E

V

E

S

T

Q

G

A

Q

L

L

I

L

T

E

Q

H

P

K

F

F

D

D

H

Y

I

I

F

F

T

T

G

G

G

S

T

P

A

R

I

V

G

G

R

K

K

I

V

V

M

M

A

T

A

A

E

K

N

H

L

L

T

T

P

P

V

I

T

T

L

L

E

E

L

L

G

G

K

K

S

N

P

P

C

C

I

Y

V

V

D

D

T

D

D

N

I

C

D

D

L

P

D

Q

V

T

A

V

A

A

R

N

R

R

I

V

A

A

W

W

G

F

K

R

F

Y

F

F

N

N

A

A

G

G

Q

Q

T

T

C

C

I

V

A

A

P

P

D

D

Y

Y

L

I

L

L

V

C

Q

S

R

Q

T

E

V

M

A

Q

E

E

P

Q

F

L

I

V

E

P

A

A

L

L

I

Q

D

N

N

A

I

I

Q

T

Q

R

F

F

Y

Y

G

G

E

D

D

N

P

P

Q

Q

Q

T

S

S

A

P

D

N

Y

L

A

G

R

R

I

I

V

I

S

N

D

Q

R

K

H

H

W

F

Q

K

R

R

L

L

N

Q

S

G

L

L

V

G

D

C

G

G

T

R

I

V

R

A

H

I

G

G

Q

Q

V

S

D

D

R

E

S

G

D

E

R

R

Y

Y

I

I

A

A

P

P

T

T

L

V

I

L

T

V

D

D

V

V

N

Q

W

E

R

T

D

E

P

P

I

V

L

M

Q

Q

E

E

I

I

F

F

G

G

P

P

L

I

L

L

P

P

I

L

L

V

I

T

Y

V

D

R

Q

S

L

L

D

D

E

E

A

A

I

I

A

E

Q

F

I

M

R

N

A

Q

Q

R

P

E

K

K

P

P

L

L

A

A

L

L

Y

Y

L

A

F

Y

S

S

R

N

D

N

R

A

Q

E

V

V

Q

I

E

K

R

Q

V

V

L

L

A

A

E

R

T

T

S

S

A

S

G

G

S

G

V

F

C

C

L

G

N

N

D

D

T

G

I

F

L

M

Q

Y

V

M

G

T

V

L

P

S

D

S

A

L

A

P

F

F

G

G

V

V

G

G

P

S

S

S

G

G

M

M

G

G

G

R

Y

Y

H

H

G

G

K

K

A

F

S

S

F

F

E

D

T

T

F

F

S

S

H

N

Y

Q

K

R

-

A

L

C

V

L

L

L

K

S

R

C

P

P

F

G

W

M

L

E

D

K

L

L

-

N

A

G

L

L

R

R

Y

Y

P

P

Y

Y

Sites not aligning to the query:

462:463 RR→AA: Reduces membrane localization.

Q8W033 Aldehyde dehydrogenase family 3 member I1, chloroplastic; AtALDH3; Ath-ALDH3; EC 1.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
46% identity, 98% coverage: 9:458/459 of query aligns to 75:529/550 of Q8W033

query
sites
Q8W033

A

A

E

L

T

L

L

V

A

D

A

E

L

L

Q

R

A

S

T

N

F

F

D

N

R

S

G

G

D

R

T

T

R

K

T

S

L

Y

A

E

F

W

R

R

L

I

A

S

R

Q

L

L

Q

Q

D

N

L

I

A

A

K

R

L

M

V

I

A

D

D

E

N

K

E

E

A

K

E
x
C

L

I

L

T

Q

E

A

A

L

L

A

Y

S

Q

D

D

L

L

R

S

K

K

P

P

A

E

L

L

E

E

A

A

Y

F

A

L

S

A

E

E

I

I

Y

S

F

N

V

T

R

K

D

S

Q

S

I
x
C

K

M

L

L

T

A

C

I

K

K

H

E

L

L

R

K

R

N

W

W

M

M

Q

A

P

P

E

E

K

T

Q

V

S

K

I

T

S

S

L

V

M

T

Q

T

Q

F

P

P

G

S

Q

S

A

A

Y

Q

R

I

Q

V

A

S

E

E

P

P

L

L

G

G

V

V

L

L

I

V

I

I

G

S

P

A

W

W

N

N

Y

F

P

P

F

F

Q

L

L

L

L

S

I

V

T

E

P

P

L

V

I

I

G

G

A

A

I

I

A

A

G

G

N

N

C

A

A

V

V

V

L

L

K

K

P

P

S

S

E

E

L

I

A

A

P

P

A

A

T

A

S

S

L

L

I

L

Q

A

R

K

L

L

I

F

S

S

D

E

R

Y

F

L

D

D

P

N

D

T

Y

T

I

I

R

R

V

V

L

I

E

E

G

G

D

G

A

V

S

P

V

E

S

T

Q

T

A

A

L

L

I

L

T

D

Q

Q

P

K

F

W

D

D

H

K

I

I

F

F

T

T

G

G

T

A

A

R

I
x
V

G

A

R

R

K

I

V

I

M

M

A

A

E

R

N

N

L

L

T

T

P

P

V

V

T

V

L

L

E

E

L

L

G

G

K

K

S
x
C

P

P

C

A

I

L

V

V

D

D

T

S

D

D

I

V

D

N

L

L

D

Q

V

V

A

A

R

R

I

I

A

I

W

A

G

G

K

K

F

W

-

A

F
x
C

N

N

A

S

G

G

Q

Q

T

A

C
|
C

I

I

A

G

P

V

D

D

Y

Y

L

V

L

I

V

T

Q

T

R

K

T

D

V

F

A

A

E

S

P

K

F

L

I

I

E

D

A

A

L

L

I

K

D

T

N

E

I

L

Q

E

Q

T

F

F

Y

F

G

G

E

Q

D

N

P

A

Q

L

Q

E

S

S

A

K

D

D

Y

L

A

S

R

R

I

I

V

V

S

N

D

S

R

F

H

H

W

F

Q

K

R

R

L

L

N

E

S

S

L

M

L

L

-

K

-

E

-

N

-

G

V

V

D

A

G

N

T

K

I

I

R

V

H

H

G

G

Q

R

V

I

D

T

R

E

S

D

D

K

R

L

Y

K

I

I

A

S

P

P

T

T

L

I

I

L

T

L

D

D

V

V

N

P

W

E

R

A

D

S

P

S

I

M

L

M

Q

Q

E

E

I

I

F

F

G

G

P

P

L

L

P

P

I

I

L

I

I

T

Y

V

D

Q

Q

K

L

I

D

E

E

D

A

G

I

F

A

Q

Q

V

I

I

R

R

A

S

Q

K

P

P

K

K

P

P

L

L

A

A

L

A

Y

Y

L

L

F

F

S

T

R

N

D

N

R

K

Q

E

V

L

Q

E

E

K

R

Q

V

F

L

V

A

Q

E

D

T

V

S

S

A

A

G

G

S

G

V

I

C

T

L

I

N

N

D

D

T

T

I

V

L

L

Q

H

V

V

G

T

V

V

P

K

D

D

A

L

A

P

F

F

G

G

V

V

G

G

P

E

S

S

G

G

M

I

G

G

G

A

Y

Y

H

H

G

G

K

K

A

F

S

S

F

Y

E

E

T

T

F

F

S

S

H

H

Y

K

K

K

L

G

V

V

L

L

K

Y

R

R

P

S

F

F

W

S

L

G

D

D

L

A

A

D

L

L

R

R

Y

Y

P

P

Y

Y

G

T

D

P

K

K

I

K

N

K

L

M

F

V

R

L

K

K

C114 (≠ E48) mutation to S: No effect on solubility, but loss of dimerization and 80% loss of activity.
C142 (≠ I76) mutation to S: No effect on solubility, but decreased activity.
V263 (≠ I197) mutation to I: No effect on substrate specificity, but decreased affinity for NADP(+) and increased affinity for NAD(+).
C286 (≠ S220) mutation to S: No effect on solubility, but no effect on activity.
C310 (≠ F243) mutation to S: No effect on solubility, but no effect on activity.
C316 (= C249) mutation to S: No effect on solubility, but loss of activity.

5ucdA Benzaldehyde dehydrogenase, a class 3 aldehyde dehydrogenase, with bound NADP+ and benzoate adduct (see paper)
42% identity, 93% coverage: 7:433/459 of query aligns to 5:431/435 of 5ucdA

query
sites
5ucdA

P

P

A

A

A

K

A

I

E

D

T

S

L

L

-

F

A

S

A

A

L

Q

Q

K

A

A

T

Y

F

F

D

A

R

T

G

R

D

A

T

T

R

A

T

D

L

V

A

G

F

F

R

R

L

K

A

Q

R

S

L

L

Q

E

D

R

L

L

A

K

K

E

L

A

V

V

A

I

D

N

N

N

E

K

A

E

E

A

L

L

L

Y

Q

S

A

A

L

L

A

A

S

E

D

D

L

L

R

G

K

K

P

P

A

K

L

D

E

V

A

V

Y

D

A

L

S

A

E

E

I

I

Y

G

F

A

V

V

R

L

D

H

Q

E

I

I

K

D

L

F

T

A

C

L

K

A

H

H

L

L

R

D

R

E

W

W

M

V

Q

A

P

P

E

V

K

S

Q

V

S

P

I

S

S

P

L

D

M

I

Q

I

Q

A

P

P

G

S

Q

E

A

C

Y

Y

R

V

Q

V

A

Q

E

E

P

P

L

Y

G

G

V

V

V

T

L

Y

I

I

I
|
I

G
|
G

P

P

W
x
F

N
|
N

Y

Y

P

P

F

V

Q

N

L

L

L

T

I

L

T

T

P

P

L

L

I

I

G

G

A

A

I

I

A

I

A

G

G

G

N

N

C

T

A

C

V

I

L

I

K
|
K

P

P

S
|
S

E
|
E

L

T

A

T

P

P

A

E

T

T

S

S

S

A

L

V

I

I

Q

E

R

K

L

I

I

I

S

A

D

E

R

A

F

F

D

A

P

P

D

E

Y

Y

I

V

R

A

V

V

L

I

E

Q

G

G

D

G

A
x
R

S

D

V

E

S

N

Q

S

A

H

L

L

I

L

T

S

Q

L

P

P

F

F

D

D

H

F

I

I

F

F

F
|
F

T
|
T

G
|
G

G
x
S

T

P

A

N

I
x
V

G

G

R

K

K

V

V

V

M

M

A

Q

A

A

E

K

N

H

L

L

T

T

P

P

V

V

T

V

L

L

E
|
E

L
|
L

G

G

K

K

S

C

P

P

C

L

I

I

V

V

D

L

T

P

D

D

I

A

D

D

L

L

D

D

V

Q

A

T

A

V

R

N

R

Q

I

L

A

M

W

F

G

G

K

K

F

F

F

I

N

N

A

S

G

G

Q

Q

T

T

C
|
C

I

I

A

A

P

P

D

D

Y

Y

L

L

L

Y

V

V

Q

H

R

Y

T

S

V

V

A

K

E

D

P

A

F

L

I

L

E

E

A

R

L

L

I

V

D

E

N

R

I

V

Q

K

Q

T

F

-

Y

-

G

-

E

E

D

L

P

P

Q

E

Q

I

S

N

A

S

D

T

Y

-

A

G

R

K

I

L

V

V

S

T

D

E

R

R

H

Q

W

V

Q

Q

R

R

L

L

N

V

S

S

L

L

-

E

-

A

V

T

D

Q

G

G

T

Q

I

V

R

L

H

V

G

G

G

S

Q

Q

V

A

D

D

R

V

S

S

D

K

R

R

Y

A

I

L

A

S

P

A

T

T

L

V

I

V

T

D

D

G

V

V

N

E

W

W

R

N

D

D

P

P

I

L

L

M

Q

S

E

E

E
|
E

I

L

F
|
F

G

G

P

P

L

I

L

L

P

P

I

V

L

L

I

E

Y

F

D

D

Q

S

L

V

D

R

E

T

A

A

I

I

A

D

Q

Q

I

V

-

N

R

K

A

H

Q

H

P

P

K

K

P

P

L

L

A

A

L

V

Y

Y

L

V

F

F

S

G

R

K

D

D

R

M

Q

D

V

V

Q

A

E

K

R

G

V

I

L

I

A

N

E

Q

T

I

S

Q

A

S

G

G

S

D

V

A

C

Q

L

V

N

N

D

G

T

V

I

M

L

L

Q

H

V

A

G

F

V

S

P

P

D

Y

A

L

A

P

F

F

G

G

V

I

G

G

P

A

S

S

G

G

M

M

G

G

G

E

Y
|
Y

H

H

G

G

K

H

A

F

S

S

F

Y

E

L

T

T

F

F

S

T

H

H

Y

K

K

K

L

S

V

V

active site: N119 (= N120), K142 (= K143), E214 (= E215), C248 (= C249), E336 (= E339), Y416 (= Y418)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I115 (= I116), G116 (= G117), F118 (≠ W119), N119 (= N120), K142 (= K143), S144 (= S145), E145 (= E146), R174 (≠ A175), F190 (= F191), T191 (= T192), G192 (= G193), S193 (≠ G194), V196 (≠ I197), E214 (= E215), L215 (= L216), C248 (= C249), E336 (= E339), F338 (= F341)

5nnoA Structure of tbaldh3 complexed with NAD and an3057 aldehyde (see paper)
40% identity, 95% coverage: 13:449/459 of query aligns to 16:472/484 of 5nnoA

query
sites
5nnoA

L

V

A

S

A

K

L

C

Q

R

A

E

T

A

F

F

D

N

R

D

G

D

D

A

T

N

R

R

T

D

L

L

A

K

F

K

R

R

L

K

A

Q

R

V

L

L

Q

R

D

S

L

L

A

L

K

N

L

L

V

V

A

E

D

E

N

N

E

T

A

D

E

E

L

F

L

C

Q

K

A

A

L

I

A

H

S

R

D

D

L

R

R

R

K

R

P

H

A

R

L

D

E

E

A

T

Y

V

A

V

S

M

E

E

I

I

Y

L

F
x
P

V

L

R

R

D

N

Q

E

I

V

K

W

L

H

T

L

C

I

K

E

H

H

L

M

R

D

R

E

W

Y

M

V

Q

K

P

P

E

V

K

K

Q

P

S

T

I

M

S

E

L

G

M

A

Q

A

P

L

G

D

Q

D

A

C

Y

E

R

L

Q

Q

A

Y

E

E

P

P

L

L

G

G

V

V

L

L

I

V

I
|
I

G
|
G

P

T

W
|
W

N
|
N

Y
|
Y

P

P

F

L

Q
x
L

L
|
L

L

I

I

L

T

Q

P

P

L

L

I

L

G

G

A

A

I

L

A

A

G

G

N

N

C

T

A

A

V

V

L

I

K
|
K

P

P

S

S

E
|
E

L

L

A

A

P

P

A

A

T

T

S

A

S

E

L

L

I

L

Q

T

R

K

L

L

I

L

S

P

D

K

R

Y

F

V

D

S

P

S

D

D

Y

V

I

V

R

G

V

I

L

V

E

N

G

G

D

G

A
x
V

S

S

V

E

S
x
T

Q

T

A

A

L

V

I

L

T

K

Q

E

P

R

F

F

D

D

H

H

I

I

F

L

F
x
Y

T
|
T

G
|
G

G
x
S

T

A

A

R

I
x
V

G

A

R

E

K

I

V

V

M

M

A

A

E

K

N

H

L

L

T

T

P

P

V

V

T

T

L

L

E
|
E

L
|
L

G

G

K

K

S

S

P

P

C

V

I

V

V

V

D

D

T

D

D

T

I

C

-

A

-

D

D

N

L

M

D

K

V

V

A

V

A

A

R

E

R

R

I

I

A

M

W

W

G

G

K

K

F

I

N

N

A

A

G

G

Q

Q

T
|
T

C
x
S

I

I

A

A

P

P

D

D

Y

Y

L

V

V

V

Q

E

R

K

T

S

V

M

A

E

E

S

P

V

F

L

I

V

E

D

A

A

L

L

I

A

D

E

N

A

I

R

Q

K

Q

A

F

M

Y

L

G

G

E

D

D

K

-

F

-

L

-

K

-

V

-

L

-

K

-

G

-

E

-

L

-

V

-

K

-

Q

P

K

Q

Q

-

F

-

L

-

E

S

E

A

S

D

D

Y

Y

A

P

R

R

I

I

V

V

S

N

D

A

R

S

H

H

W

F

Q

Q

R

R

L

L

N

M

S

E

L

F

L

M

V

K

D

G

G

G

T

K

I

V

R

A

H

V

G

G

Q

E

V

A

D

D

R

E

S

A

D

T

R

L

Y

T

I

I

A

A

P

P

T

T

L

I

I

L

T

T

D

N

V

I

N

D

W

P

R

T

D

H

P

P

I

V

L

M

Q

Q

E

E

E
|
E

I

I

F
|
F

G

G

P

P

L

I

L

L

P

P

I

V

L

L

I

T

Y

Y

D

E

Q

N

L

E

D

K

E

D

A

I

I

L

A

K

Q

I

I

I

R

N

A

S

Q

R

P

E

K

K

P

P

L

L

A

A

L

L

Y

Y

L

V

F

F

S

S

R

N

D

N

R

K

Q

R

V

F

Q

I

E

R

R

G

V

V

L

E

A

S

E

R

T

T

S

S

A

S

G

G

S

A

V

V

C

V

L

V

N

N

D

D

T

V

I

V

L

V

Q

H

V

A

G
|
G

V

A

P

D

D

G

A

L

A

P

F
|
F

G

G

V

V

G

G

P

R

S

S

G

G

M

M

G

G

G

A

Y
|
Y

H

H

G

G

K

R

A

Y

S

S

F

F

E

E

T

T

F

F

S

S

H

H

Y

R

K

R

L

P

V

V

L

M

K

R

R

R

P

G

F

F

W

L

L

F

D

S

L

S

-

I

-

D

A

T

L

V

R

R

Y

F

P

P

Y

Y

active site: N123 (= N120), K146 (= K143), E218 (= E215), S254 (≠ C249), E360 (= E339), Y439 (= Y418)
binding 4-[(1-oxidanyl-3~{H}-2,1-benzoxaborol-5-yl)oxy]benzaldehyde: P74 (≠ F71), Y124 (= Y121), L127 (≠ Q124), T253 (= T248), S254 (≠ C249), G422 (= G401)
binding nicotinamide-adenine-dinucleotide: I119 (= I116), G120 (= G117), W122 (= W119), N123 (= N120), L128 (= L125), K146 (= K143), E149 (= E146), V178 (≠ A175), T181 (≠ S178), Y194 (≠ F191), T195 (= T192), G196 (= G193), S197 (≠ G194), V200 (≠ I197), E218 (= E215), L219 (= L216), S254 (≠ C249), E360 (= E339), F362 (= F341), F428 (= F407)

Q04458 Fatty aldehyde dehydrogenase HFD1; Hexadecenal dehydrogenase; EC 1.2.1.3; EC 1.2.1.64 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
34% identity, 92% coverage: 26:448/459 of query aligns to 44:481/532 of Q04458

query
sites
Q04458

R

K

T

D

L

L

A

E

F

F

R

R

L

Q

A

L

R

Q

L

L

Q

K

D

K

L

L

A

Y

K

Y

L

A

V

V

A

K

D

D

N

H

E

E

A

E

E

E

L

L

L

I

Q

D

A

A

L

M

A

Y

S

K

D

D

L

F

R

H

K

R

P

N

A

K

L

I

E

E

A

S

Y

V

A

L

S

N

E

E

I

T

Y

T

F

K

V

L

R

M

D

N

Q

D

I

I

K

L

L

H

T

L

C

I

K

E

H

I

L

L

R

P

R

K

W

L

M

I

Q

K

P

P

E

R

K

R

Q

V

S

S

I

D

S

S

L

-

M

-

Q

S

Q

P

P

P

-

F

-

M

-

F

G

G

Q

K

A

T

Y

I

R

V

Q

E

A

K

E

I

P

S

L

R

G

G

V

S

V

V

L

L

I

I

G

A

P

P

W

F

N

N

Y

F

P

P

F

L

Q

L

L

L

L

A

I

F

T

A

P

P

L

L

I

A

G

A

A

A

I

L

A

A

G

G

N

N

C

T

A

I

V

V

L

L

K

K

P

P

S

S

E

E

L

L

A

T

P

P

A

H

T

T

S

A

S

V

L

V

I

M

Q

E

R

N

L

L

I

L

S

T

D

T

R

A

F

G

D

F

P

P

D

D

-

G

Y

L

I

I

R

Q

V

V

L

V

E

Q

G

G

D

A

A

I

S

D

V

E

S

T

Q

T

A

R

L

L

I

L

-

D

T

C

Q

G

P

K

F

F

D

D

H

L

I

I

F

F

F

Y

T

T

G

G

G

S

T

P

A

R

I

V

G

G

R

S

K

I

V

V

M

A

A

E

A

K

A

A

E

K

N

S

L

L

T

T

P

P

V

C

T

V

L

L

E

E

L

L

G

G

K

K

S
|
S

P

P

C

T

I

F

V

I

D

T

T

E

D

N

I

F

-

K

-

A

-

S

D

N

L

I

D

K

V

I

A

A

A

L

R

K

R

R

I

I

A

F

W

F

G

G

K

A

F

F

F

G

N

N

A

S

G

G

Q

Q

T

I

C
|
C

I

V

A

S

P

P

D

D

Y

Y

L

L

V

V

Q

H

R

K

T

S

V

I

A

Y

E

P

P

K

F

V

I

I

E

K

A

E

L

C

I

E

D

S

N

V

I

L

Q

N

Q

E

F

F

Y

Y

G

P

E

S

D

F

P

D

Q

E

Q

Q

S

T

A

-

D

D

Y

F

A

T

R

R

I

M

V

I

S

H

D

E

R

P

H

A

W

Y

Q

K

R

K

-

A

-

V

-

A

-

S

L

I

N

N

S

S

L

T

L

N

V

G

D

S

G

K

T

I

I

V

R

P

H

S

G

K

G

I

Q

S

V

I

D

N

R

S

S

D

D

T

R

E

-

D

-

L

-

C

Y

L

I

V

A

P

P

P

T

T

L

I

I

V

T

Y

D

N

V

I

N

G

W

W

R

D

D

D

P

P

I

L

L

M

Q

K

E

Q

E

E

I

N

F

F

G

A

P

P

L

V

L

L

P

P

I

I

L

I

I

E

Y

Y

D

E

Q

D

L

L

D

D

E

E

A

T

I

I

A

N

Q

K

I

I

-

I

R

E

A

E

Q

H

P

D

K

T

P

P

L

L

A

V

L

Q

Y

Y

L

I

F

F

S

S

R

D

D

S

R

Q

Q

T

V

E

Q

I

E

N

R

R

V

I

L

L

A

T

E

R

T

L

S

R

A

S

G

G

S

D

V

C

C

V

L

V

N

G

D

D

T

T

I

V

L

I

Q

H

V

V

G

G

V

I

P

T

D

D

A

A

A

P

F

F

G

G

V

I

G

G

P

T

S

S

G

G

M

Y

G

G

G

N

Y

Y

H

G

G

G

K

Y

A

Y

S

G

F

F

E

N

T

T

F

F

S

S

H

H

Y

E

K

R

L

T

V

I

L

F

K

K

R

Q

P

P

F

Y

W

W

L

N

D

D

L

F

A

T

L

L

-

F

-

M

R

R

Y

Y

P

P

S241 (= S220) mutation to L: Causes Q deficiency.
C273 (= C249) mutation to S: Abolishes catalytic activity.

P17202 Aminoaldehyde dehydrogenase BADH; 4-trimethylammoniobutyraldehyde dehydrogenase BADH; Aminobutyraldehyde dehydrogenase BADH; Betaine aldehyde dehydrogenase; SoBADH; EC 1.2.1.-; EC 1.2.1.47; EC 1.2.1.19; EC 1.2.1.8 from Spinacia oleracea (Spinach) (see 3 papers)
33% identity, 75% coverage: 90:435/459 of query aligns to 129:484/497 of P17202

query
sites
P17202

E

Q

K

K

Q

A

S

P

I

V

S

T

L

L

M

P

Q

M

Q

E

P

R

G

F

Q

K

A

S

Y

H

R

V

Q

L

A

R

E

Q

P

P

L

L

G

G

V

V

L

G

I

L

I

I

G
x
S

P
|
P

W
|
W

N

N

Y
|
Y

P

P

F

L

Q

L

L

M

L

A

I

T

T
x
W

P

K

L

I

I

A

G

P

A

A

I

L

A

A

G

G

N

C

C

T

A

A

V

V

L

L

K
|
K

P
|
P

S
|
S

E
|
E

L
|
L

A

A

P

S

A

V

T

T

S

C

S

L

L

E

I

F

Q

G

R

E

L

V

I

C

S

N

D

E

-

V

R

G

F

L

D

P

P

P

D

G

Y

V

I

L

R

N

V

I

L

L

E

T

G

G

D

L

A

G

S

P

V

D

S

A

Q

G

A

A

-

P

L

L

I

V

T

S

Q

H

P

P

-

D

F

V

D

D

H

K

I

I

F

A

F

F

T

T

G

G

G
x
S

T
x
S

A
|
A

I
x
T

G

G

R

S

K

K

V

V

M

M

A

A

A

S

A

A

E

Q

N

L

L
x
V

T

K

P

P

V

V

T

T

L

L

E

E

L
|
L

G

G

K

K

S

S

P

P

C

I

I

V

V

V

D

F

T

E

D

D

I

V

D

D

L

I

D

D

V

K

A

V

R

E

R

W

I

T

A

I

W

F

G

G

K

C

F

F

F
x
W

N

T

A

N

G

G

Q

Q

T

I

C

C

I

S

A

A

P

T

D

S

Y

R

L

L

V

V

Q

H

R

E

T

S

V

I

A

A

E

A

P

E

F

F

I

V

E

D

A

K

L

L

I

V

D

K

N

W

I

T

Q

K

Q

N

F

I

Y

K

G

I

E

S

D

D

P

P

-

F

Q

E

S

G

A

C

D

R

Y

L

A

G

R

P

I

V

V

I

S

S

D

K

R

G

H

Q

W

Y

Q

D

R

K

L

I

N

M

S

K

L

F

L

I

V

S

D

T

-

A

-

K

-

S

-

E

-

G

G

A

T

T

I

I

R

L

H

Y

G

G

Q

S

V

R

D

P

-

E

-

H

-

L

R

K

S

K

D

G

R

Y

Y

Y

I

I

A

E

P

P

T

T

L

I

I

V

T

T

D

D

V

I

N

S

W

T

R

S

D

M

P

Q

I

I

L

W

Q

K

E

E

E
|
E

I

V

F

F

G

G

P

P

L

V

L

L

P

C

I

V

L

K

I

T

Y

F

D

S

Q

S

L

E

D

D

E

E

A

A

I

I

A

A

Q

L

I

A

R

N

A

D

Q

T

P

E

K

Y

P

G

L

L

A

A

L

A

Y

A

L

V

F

F

S

S

R

N

D

D

R

L

Q

E

V

R

Q

C

E

E

R

R

V

I

L

T

A

K

E

A

T

L

S

E

A

V

G

G

S
x
A

V

V

C

W

L

V

N

N

D

C

T

S

I

-

L

-

Q

Q

V

P

G
x
C

V

F

P

V

D

Q

A

A

A

P

F
x
W

G

G

V

I

G
x
K

P

R

S

S

G

G

M

F

G

G

G

R

Y

E

H

L

G

G

K

E

A

W

S

G

F

I

E

Q

T

N

F

Y

S

L

H

N

Y

I

K

K

L

Q

V

V

L

T

K

Q

SPW 156:158 (≠ GPW 117:119) binding
Y160 (= Y121) mutation to A: Decreases binding affinity for betaine aldehyde; increases binding affinity for 4-(trimethylamino)butanal.
W167 (≠ T128) mutation to A: Decreases binding affinity for betaine aldehyde; increases binding affinity for 4-aminobutanal.
KPSE 182:185 (= KPSE 143:146) binding
L186 (= L147) binding
SSAT 236:239 (≠ GTAI 194:197) binding
V251 (≠ L209) binding in other chain
L258 (= L216) binding
W285 (≠ F243) mutation to A: Decreases binding affinity for betaine aldehyde.
E390 (= E339) binding
A441 (≠ S390) mutation to I: Decreases binding affinity for betaine aldehyde; increases binding affinity for 4-aminobutanal.
C450 (≠ G401) mutation to S: Loss of partial inactivation by betaine aldehyde in the absence of NAD(+).
W456 (≠ F407) binding ; mutation to A: Decreases binding affinity for betaine aldehyde.
K460 (≠ G411) binding

Sites not aligning to the query:

28 binding
96 binding

4v37A Crystal structure of betaine aldehyde dehydrogenase from spinach showing a thiohemiacetal with 3-aminopropionaldehyde
33% identity, 75% coverage: 90:435/459 of query aligns to 127:482/495 of 4v37A

query
sites
4v37A

E

Q

K

K

Q

A

S

P

I

V

S

T

L

L

M

P

Q

M

Q

E

P

R

G

F

Q

K

A

S

Y

H

R

V

Q

L

A

R

E

Q

P

P

L

L

G

G

V

V

L

G

I

L

I
|
I

G
x
S

P
|
P

W
|
W

N
|
N

Y

Y

P

P

F

L

Q

L

L
x
M

L

A

I

T

T

W

P

K

L

I

I

A

G

P

A

A

I

L

A

A

G

G

N

C

C

T

A

A

V

V

L

L

K
|
K

P

P

S
|
S

E
|
E

L

L

A

A

P

S

A

V

T

T

S

C

S

L

L

E

I

F

Q

G

R

E

L

V

I

C

S

N

D

E

-

V

R

G

F

L

D

P

P

P

D

G

Y

V

I

L

R

N

V

I

L

L

E

T

G

G

D

L

A
x
G

S

P

V

D

S

A

Q
x
G

A
|
A

-

P

L

L

I

V

T

S

Q

H

P

P

-

D

F

V

D

D

H

K

I

I

F

A

F

F

T
|
T

G
|
G

G
x
S

T

S

A

A

I
x
T

G

G

R

S

K

K

V

V

M

M

A

A

A

S

A

A

E

Q

N

L

L

V

T

K

P

P

V

V

T

T

L

L

E
|
E

L
|
L

G

G

K

K

S

S

P

P

C

I

I

V

V

V

D

F

T

E

D

D

I

V

D

D

L

I

D

D

V

K

A

V

R

E

R

W

I

T

A

I

W

F

G

G

K

C

F

F

F

W

N

T

A

N

G

G

Q

Q

T

I

C
x
A

I

S

A

A

P

T

D

S

Y

R

L

L

V

V

Q

H

R

E

T

S

V

I

A

A

E

A

P

E

F

F

I

V

E

D

A

K

L

L

I

V

D

K

N

W

I

T

Q

K

Q

N

F

I

Y

K

G

I

E

S

D

D

P

P

-

F

Q

E

S

G

A

C

D

R

Y

L

A

G

R

P

I

V

V

I

S

S

D

K

R

G

H

Q

W

Y

Q

D

R

K

L

I

N

M

S

K

L

F

L

I

V

S

D

T

-

A

-

K

-

S

-

E

-

G

G

A

T

T

I

I

R

L

H

Y

G

G

Q

S

V

R

D

P

-

E

-

H

-

L

R

K

S

K

D

G

R

Y

Y

Y

I

I

A

E

P

P

T

T

L

I

I

V

T

T

D

D

V

I

N

S

W

T

R

S

D

M

P

Q

I

I

L

W

Q

K

E

E

E
|
E

I

V

F
|
F

G

G

P

P

L

V

L

L

P

C

I

V

L

K

I

T

Y

F

D

S

Q

S

L

E

D

D

E

E

A

A

I

I

A

A

Q

L

I

A

R

N

A

D

Q

T

P

E

K

Y

P

G

L

L

A

A

L

A

Y

A

L

V

F

F

S

S

R

N

D

D

R

L

Q

E

V

R

Q

C

E

E

R

R

V

I

L

T

A

K

E

A

T

L

S

E

A

V

G

G

S

A

V

V

C

W

L

V

N

N

D

C

T

S

I

-

L

-

Q

Q

V

P

G
x
C

V

F

P

V

D

Q

A

A

A

P

F
x
W

G

G

V

I

G

K

P

R

S

S

G

G

M

F

G

G

G

R

Y
x
E

H

L

G

G

K

E

A

W

S

G

F

I

E

Q

T

N

F

Y

S

L

H

N

Y

I

K

K

L

Q

V

V

L

T

K

Q

active site: N157 (= N120), K180 (= K143), E255 (= E215), A289 (≠ C249), E388 (= E339), E465 (≠ Y418)
binding 3-aminopropan-1-ol: C448 (≠ G401), W454 (≠ F407)
binding nicotinamide-adenine-dinucleotide: I153 (= I116), S154 (≠ G117), P155 (= P118), W156 (= W119), N157 (= N120), M162 (≠ L125), K180 (= K143), S182 (= S145), E183 (= E146), G213 (≠ A175), G217 (≠ Q179), A218 (= A180), T232 (= T192), G233 (= G193), S234 (≠ G194), T237 (≠ I197), E255 (= E215), L256 (= L216), A289 (≠ C249), E388 (= E339), F390 (= F341)

Q9HTJ1 NAD/NADP-dependent betaine aldehyde dehydrogenase; BADH; EC 1.2.1.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
34% identity, 72% coverage: 104:433/459 of query aligns to 137:476/490 of Q9HTJ1

query
sites
Q9HTJ1

Y

Y

R

T

Q

R

A

R

E

E

P

P

L

L

G

G

V

V

L

A

I

G

I

I

G
|
G

P
x
A

W
|
W

N
|
N

Y

Y

P

P

F

V

Q

Q

L

I

L

A

I

L

T

W

P
x
K

L

S

I

A

G

P

A

A

I

L

A

A

G

G

N

N

C

A

A

M

V

I

L

F

K
|
K

P
|
P

S
|
S

E
|
E

L

V

A

T

P

P

A

L

T

T

S

A

S

L

L

K

I

L

Q

A

R

E

L

I

I

Y

S

T

D

E

R

A

F

G

D

V

P

P

D

D

Y

G

I

V

-

F

R

N

V

V

L

L

E

T

G

G

D

S

A
x
G

-

R

S

E

V

V

S

G

Q

Q

A

W

L

L

I

T

T

E

Q

H

P

P

F

L

-

I

D

E

H

K

I

I

F

S

F

F

T

T

G

G

G
|
G

T
|
T

A
x
S

I
x
T

G

G

R

K

K

K

V

V

M

M

A

A

-

S

A

A

A

S

E

S

N

S

L

L

T

K

P

E

V

V

T

T

L

M

E
|
E

L

L

G

G

K

K

S

S

P

P

C

L

I

I

V

I

D

F

T

P

D

D

I

A

D

D

L

L

D

D

V

R

A

A

R

D

R

I

I

A

A

V

W

M

G

A

K

N

F

F

N

S

A

S

G

G

Q

Q

T

V

C
|
C

I

T

A

N

P

G

D

T

Y

R

L

V

L

F

V

I

Q

H

R

R

T

S

V

Q

A

Q

E

A

P

R

F

F

I

E

E

A

A

K

L

V

I

L

D

E

N

R

I

V

Q

Q

Q

R

F

I

Y

R

G

L

E

G

D

D

P

P

Q

Q

-

D

Q

E

S

N

A

T

D

N

Y

F

A

G

R

P

I

L

V

V

S

S

D

F

R

P

H

H

W

M

Q

E

R

S

L

V

N

L

S

G

L

Y

L

I

V

E

D

S

G

G

T

K

I

A

R

Q

H

K

G

A

G

R

Q

L

V

L

D

C

R

G

S

G

D

E

R

R

-

V

-

T

-

D

-

G

-

A

-

F

-

G

-

K

-

G

-

A

Y

Y

I

V

A

A

P

P

T

T

L

V

I

F

T

T

D

D

V

C

N

R

W

D

R

D

D

M

P

T

I

I

L

V

Q

R

E

E

E
|
E

I

I

F

F

G

G

P

P

L

V

L

M

P

S

I

I

L

L

I

V

Y

Y

D

D

Q

D

L

E

D

D

E

E

A

A

I

I

A

R

Q

R

I

A

R

N

A

D

Q

T

P

E

K

Y

P

G

L

L

A

A

L

A

Y

G

L

V

F

V

S

T

R

Q

D

D

R

L

Q

A

V

R

Q

A

E

H

R

R

V

A

L

I

A

H

E

R

T

L

S

E

A

A

G

G

S

-

V

I

C

C

L

W

N

I

D

N

T

T

I

W

L

G

Q

E

V

-

G

S

V

P

P

A

D

E

A

M

A

P

F

V

G

G

V

Y

G

K

P

Q

S

S

G

G

M

V

G

G

G

R

Y
x
E

H

N

G

G

K

-

A

-

S

-

F

L

E

T

T

T

F

L

S

A

H

H

Y

Y

K

T

L

R

V

I

GAWN 150:153 (≠ GPWN 117:120) binding
K162 (≠ P129) active site, Charge relay system
KPSE 176:179 (= KPSE 143:146) binding
G209 (≠ A175) binding
GTST 230:233 (≠ GTAI 194:197) binding
E252 (= E215) active site, Proton acceptor
C286 (= C249) binding covalent; modified: Cysteine sulfenic acid (-SOH)
E387 (= E339) binding
E464 (≠ Y418) active site, Charge relay system

Query Sequence

>Synpcc7942_0489 Synpcc7942_0489 aldehyde dehydrogenase
MTAVVLPAAAETLAALQATFDRGDTRTLAFRLARLQDLAKLVADNEAELLQALASDLRKP
ALEAYASEIYFVRDQIKLTCKHLRRWMQPEKQSISLMQQPGQAYRQAEPLGVVLIIGPWN
YPFQLLITPLIGAIAAGNCAVLKPSELAPATSSLIQRLISDRFDPDYIRVLEGDASVSQA
LITQPFDHIFFTGGTAIGRKVMAAAAENLTPVTLELGGKSPCIVDTDIDLDVAARRIAWG
KFFNAGQTCIAPDYLLVQRTVAEPFIEALIDNIQQFYGEDPQQSADYARIVSDRHWQRLN
SLLVDGTIRHGGQVDRSDRYIAPTLITDVNWRDPILQEEIFGPLLPILIYDQLDEAIAQI
RAQPKPLALYLFSRDRQVQERVLAETSAGSVCLNDTILQVGVPDAAFGGVGPSGMGGYHG
KASFETFSHYKLVLKRPFWLDLALRYPPYGDKINLFRKL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory