SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Synpcc7942_1024 FitnessBrowser__SynE:Synpcc7942_1024 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

D7A5Q8 Aromatic amino acid exporter YddG from Starkeya novella (strain ATCC 8093 / DSM 506 / JCM 20403 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIMB 10456) (see paper)
32% identity, 53% coverage: 78:252/330 of query aligns to 62:234/287 of D7A5Q8

query
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D7A5Q8

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Y78 (= Y94) mutation to A: Shows moderate effects on the transport activities of both threonine and methionine.
H79 (≠ N95) mutation to A: Abolishes the transport activities for both threonine and methionine.
Y82 (≠ F98) mutation to A: Enhances methionine transport and slightly reduces threonine transport.
W101 (≠ N117) mutation to A: Exhibits decreased transport activity for threonine, but not for methionine.
W163 (= W177) mutation to A: Exhibits decreased transport activity for threonine, but not for methionine.

Query Sequence

>Synpcc7942_1024 FitnessBrowser__SynE:Synpcc7942_1024
MGDDQSDRLRCAGLPLKFPVSLQLAIATALWGGTFTAGRIAVQQLSPLAVACGRYLLATT
VLLLILWQREGWPPLNRRQQLLLFGLGVSGIALYNWLFFIGLSLIPASRAALIIALNPTA
IALGAAIWTGDRLRSWQWAGVGLSLIGAILLLGSRQAGALTLPGWGDLALVGCVLCWTVY
SLLARQALRSLSPLTVTTGACCWGSVLLIGLWLGQGAQLPVNVSFSTGSAIAFLGLGGTA
LAFCLYANGIERLGAARAGLFINLVPVFGSAIGALLLQEPLSGLTLLGGLLVLAGVGLGT
LQRLQPVPISTTEPVGGDRSPGPPALGHDR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory