SitesBLAST

G98 (= G11) binding
F99 (= F12) binding
V100 (≠ I13) binding
D119 (= D32) binding
N120 (= N33) binding
F122 (= F35) binding
T123 (= T36) binding
G124 (= G37) binding
D144 (= D57) binding
V145 (≠ I58) binding
L149 (≠ I62) binding
Y150 (≠ R63) binding
L159 (= L72) binding
S161 (≠ C74) binding
K177 (= K90) binding
T178 (= T91) binding
N185 (= N98) binding
G188 (= G101) binding
K191 (= K104) binding
R192 (= R105) binding
A200 (= A113) binding
E204 (= E117) mutation to A: Reduced UDP-glucuronic acid decarboxylase activity.
Y231 (= Y144) active site, Proton acceptor; binding ; mutation to F: Abolished UDP-glucuronic acid decarboxylase activity.
K235 (= K148) binding
R236 (= R149) mutation to H: Strongly reduced UDP-glucuronic acid decarboxylase activity, caused by a local unfolding of the active site that allows for a rotation of the dimer interface, leading to the formation of a homohexamer.
Y245 (= Y158) binding
Q248 (= Q161) binding
E249 (≠ H162) binding
T261 (= T174) binding
H267 (≠ L180) binding
R272 (= R185) binding
R361 (= R274) mutation to Q: Strongly reduced UDP-glucuronic acid decarboxylase activity.

Sites not aligning to the query:

1 modified: N-acetylmethionine

2b69A Crystal structure of human udp-glucoronic acid decarboxylase
65% identity, 95% coverage: 3:312/325 of query aligns to 3:312/312 of 2b69A

query
sites
2b69A

R

R

I

I

L

L

V

I

T

T

G
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G

G

G

A

A

G
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G

F
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F

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x
V

G

G

S

S

H

H

L

L

I

T

D

D

R

K

L

L

M

M

S

M

A

D

G

G

H

H

E

E

V

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I

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C

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L

V

D
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D

N
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N

Y

F

F

F

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T

G
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G

R

R

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K

H

R

N

N

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Y

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M

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Y

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N

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K

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K

T
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F

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N

M

M

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G
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G

L
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L

A

A

K
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K

R
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R

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G

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A

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A

A

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E

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Y

G

G

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D

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H

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D

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K

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I
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I

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N

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F

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G

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R

M

M

L

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M

N

N

D

D

G

G

R
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R

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V

V

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N

F

F

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I

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A

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L

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G

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T

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Y

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R

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A

F

F

C

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Y

Y

V

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S

D

D

L

L

V

V

D

N

G

G

L

L

I

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R

A

L

L

M

M

N

N

G

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D

N

H

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G

S

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V

N

N

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G

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I

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A

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A

active site: T115 (= T115), S116 (= S116), E117 (= E117), Y144 (= Y144), K148 (= K148), R185 (= R185)
binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), F12 (= F12), V13 (≠ I13), D32 (= D32), N33 (= N33), T36 (= T36), G37 (= G37), D57 (= D57), V58 (≠ I58), L72 (= L72), A73 (= A73), S74 (≠ C74), A76 (= A76), T91 (= T91), T115 (= T115), Y144 (= Y144), K148 (= K148), I171 (= I171), N173 (= N173), R185 (= R185)
binding uridine-5'-diphosphate: P61 (= P61), L62 (≠ I62), Y63 (≠ R63), P78 (= P78), N98 (= N98), G101 (= G101), L102 (= L102), K104 (= K104), R105 (= R105), Y158 (= Y158), N173 (= N173), R185 (= R185), V186 (= V186), N189 (= N189), T201 (= T201), Y203 (= Y203), Q208 (= Q208), R210 (= R210), I244 (= I244), D270 (= D270)

Q6GMI9 UDP-glucuronic acid decarboxylase 1; UDP-glucuronate decarboxylase 1; UXS-1; EC 4.1.1.35 from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
66% identity, 93% coverage: 3:304/325 of query aligns to 88:389/418 of Q6GMI9

query
sites
Q6GMI9

R

R

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I

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T

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D

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N

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N

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M

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N

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F

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Q

A

A

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G

G

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A

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Y

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D

L

L

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N

G

G

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L

L

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M

N

N

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N

H

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P

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V

N

N

L

L

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G

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N

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T

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A

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L

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S

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F

R234 (= R149) mutation to H: In Man o' war (mow) mutant; characterized by severe craniofacial defects. Fishes show defective organization and morphogenesis of chondrocytes, perichondrium and bone.

4lk3B Crystal structure of human udp-xylose synthase r236a substitution (see paper)
56% identity, 95% coverage: 3:312/325 of query aligns to 3:274/274 of 4lk3B

query
sites
4lk3B

R

R

I

I

L

L

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I

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G
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G

G

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G

F
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F

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V

G

G

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H

L

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D

D

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G

H

H

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D

N
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N

Y

F

F

F

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T

G
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G

R

R

K

K

H

R

N

N

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V

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E

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I

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G

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F

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D

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L

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S

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P

A

A

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H

-

Y

-

Q

-

Y

Y

N

N

P

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I
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I

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K

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K
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K

T
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F

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G

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N

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G

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I

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F

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x
H

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N

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D

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N

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F

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R

S

A

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F

C

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Y

V

V

S

S

D

D

L

L

V

V

D

N

G

G

L

L

I

V

R

A

L

L

M

M

N

N

G

S

D

N

H

V

L

S

G

S

P

P

V

V

N

N

L

L

G

G

N

N

P

P

S

E

E

E

Y

H

T

T

I
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I

L

L

Q

E

L

F

A

A

E

Q

L

L

I

I

R

K

D

N

R

L

I

V

D

G

P

S

A

G

L

S

P

E

I

I

E

Q

F

F

R

L

P

-

L

-

P

-

Q

-

D

-

P

-

Q

-

R

R

R

K

P

P

D

D

I

I

S

K

R

K

A

A

Q

K

A

L

W

M

L

L

K

G

W

W

Q

E

P

P

L

V

V

V

S

P

V

L

Q

E

D

E

G

G

L

L

D

N

R

K

T

A

I

I

A

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D

Y

F

F

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R

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K

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L

Q

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A

Y

A

Q

A

A

active site: T112 (= T115), S113 (= S116), E114 (= E117), K119 (= K148), R156 (= R185)
binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), F12 (= F12), V13 (≠ I13), D32 (= D32), N33 (= N33), T36 (= T36), G37 (= G37), D57 (= D57), V58 (≠ I58), L72 (= L72), A73 (= A73), S74 (≠ C74), P75 (= P75), T88 (= T91), A110 (= A113), T112 (= T115), K119 (= K148), I142 (= I171), H151 (≠ L180)
binding uridine-5'-diphosphate: R156 (= R185), V157 (= V186), N160 (= N189), T172 (= T201), Y174 (= Y203), Q179 (= Q208), R181 (= R210), I215 (= I244)
binding uridine-5'-diphosphate-glucuronic acid: P61 (= P61), L62 (≠ I62), Y63 (≠ R63), I83 (= I86), K87 (= K90), N95 (= N98), G98 (= G101), L99 (= L102), K101 (= K104), Y129 (= Y158), E133 (≠ H162)

4lk3C Crystal structure of human udp-xylose synthase r236a substitution (see paper)
55% identity, 95% coverage: 3:312/325 of query aligns to 3:271/271 of 4lk3C

query
sites
4lk3C

R

R

I

I

L

L

V

I

T

T

G
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G

G

G

A

A

G
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G

F
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F

I
x
V

G

G

S

S

H

H

L

L

I

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D

D

R

K

L

L

M

M

S

M

A

D

G

G

H

H

E

E

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V

I

T

C

V

L

V

D
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D

N
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N

Y

F

F

F

T
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T

G
|
G

R

R

K

K

H

R

N

N

V

V

A

E

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H

W

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Y

I

G

G

H

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F

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E

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L

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H

D
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D

I
x
V

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P

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x
L

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x
Y

L

I

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E

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V

D

D

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Q

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Y

H

H

L
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L

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C
x
S

P
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P

A

A

S

S

P

-

V

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H

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Y

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Q

-

Y

Y

N

N

P

P

I

I

K

K

T

T

A

L

K

K

T
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T

S

N

F

T

L

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G

G

T

T

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L

N
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N

M

M

L

L

G
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G

L
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L

A

A

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K

R
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R

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G

A

A

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L

L

M

L

A

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T

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E
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E

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Y

G

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D

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Y

H

M

R

K

Q

Q

H
x
E

N

G

L

V

E

E

I

V

R

R

V

V

A

A

R

R

I
|
I

F

F

N

N

T

T

Y

F

G

G

P

P

R

R

M

M

L
x
H

E

M

N

N

D

D

G

G

R
|
R

V
|
V

V

V

S

S

N

N

F

F

I

I

V

L

Q

Q

A

A

L

L

Q

Q

G

G

Q

E

P

P

L

L

T

T

V

V

Y

Y

G

G

R

S

G

G

E

S

Q

Q

T

T

R

R

S

A

F

F

C

Q

Y

Y

V

V

S

S

D

D

L

L

V

V

D

N

G

G

L

L

I

V

R

A

L

L

M

M

N

N

G

S

D

N

H

V

L

S

G

S

P

P

V

V

N

N

L

L

G

G

N

N

P

P

S

E

E

E

Y

H

T

T

I

I

L

L

Q

E

L

F

A

A

E

Q

L

L

I

I

R

K

D

N

R

L

I

V

D

G

P

S

A

G

L

S

P

E

I

I

E

Q

F

F

R

-

P

-

L

-

P

-

Q

-

D

-

P

-

Q

-

R

L

R

K

P

P

D

D

I

I

S

K

R

K

A

A

Q

K

A

L

W

M

L

L

K

G

W

W

Q

E

P

P

L

V

V

V

S

P

V

L

Q

E

D

E

G

G

L

L

D

N

R

K

T

A

I

I

A

H

D

Y

F

F

R

R

D

K

R

E

Q

L

Q

E

A

Y

A

Q

A

A

active site: T110 (= T115), S111 (= S116), E112 (= E117), K117 (= K148), R154 (= R185)
binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), F12 (= F12), V13 (≠ I13), D32 (= D32), N33 (= N33), T36 (= T36), G37 (= G37), D57 (= D57), V58 (≠ I58), L72 (= L72), A73 (= A73), S74 (≠ C74), P75 (= P75), T86 (= T91), K117 (= K148), I140 (= I171), H149 (≠ L180)
binding pyrophosphate 2-: R154 (= R185), V155 (= V186)
binding uridine-5'-diphosphate-glucuronic acid: P61 (= P61), L62 (≠ I62), Y63 (≠ R63), N93 (= N98), G96 (= G101), L97 (= L102), K99 (= K104), R100 (= R105), Y127 (= Y158), E131 (≠ H162)

4m55E Crystal structure of human udp-xylose synthase r236h substitution (see paper)
46% identity, 72% coverage: 3:236/325 of query aligns to 1:156/164 of 4m55E

query
sites
4m55E

R

R

I

I

L

L

V

I

T

T

G
|
G

G

G

A

A

G
|
G

F
|
F

I
x
V

G

G

S

S

H

H

L

L

I

T

D

D

R

K

L

L

M

M

S

M

A

D

G

G

H

H

E

E

V

V

I

T

C

V

L

V

D
|
D

N
|
N

Y
x
F

F

F

T
|
T

G
|
G

R

R

K

K

H

R

N

N

V

V

A

E

Q

H

W

W

Y

I

G

G

H

H

P

E

R

N

F

F

E

E

L

L

I

I

R

N

H

H

D
|
D

I
x
V

T

V

D

E

P

P

I

L

R

Y

L

I

E

E

V

V

D

D

Q

Q

I

I

Y

Y

H

H

L

L

A

A

C
x
S

P
|
P

A

-

S

-

P

-

V

-

H

-

Y

-

Q

-

Y

-

N

N

P

P

I

I

K

K

T

T

A

L

K

K

T
|
T

S

N

F

T

L

I

G

G

T

T

V

L

N

N

M

M

L

L

G

G

L

L

A

A

K

K

R

R

V

V

K

G

A

A

R

R

L

L

M

L

A

A

S

S

T
|
T

S

S

E

-

V

-

Y

-

G

-

D

-

P

-

H

-

V

-

H

-

P

-

Q

-

T

-

E

-

D

-

Y

-

W

-

G

-

N

-

V

-

N

-

P

-

I

-

G

-

I

-

R

-

S

-

C

-

Y

-

D

-

E

-

G

-

K

-

R

-

V

V

A

A

E

E

T

T

L

M

C

C

F

Y

D

A

Y

Y

H

M

R

K

Q

Q

H

E

N

G

L

V

E

E

I

V

R

R

V

V

A

A

R

R

I

I

F

F

N

N

T
|
T

Y

-

G

-

P

-

R

-

M

-

L

-

E

-

N

-

D

-

G

-

R

-

V

-

S

-

N

-

F

-

I

-

V

-

Q

-

A

-

L

-

Q

-

G

-

Q

-

P

-

L

-

T

-

V

-

Y

-

G

-

R

-

G

-

E

-

Q

-

T

-

R

-

S

-

F

F

C

Q

Y

Y

V

V

S

S

D

D

L

L

V

V

D

N

G

G

L

L

I

V

R

A

L

L

M

M

N

N

G

S

D

N

H

V

L

S

G

S

P

P

V

V

N

N

L

L

active site: T105 (= T115)
binding nicotinamide-adenine-dinucleotide: G6 (= G8), G9 (= G11), F10 (= F12), V11 (≠ I13), D30 (= D32), N31 (= N33), F32 (≠ Y34), T34 (= T36), G35 (= G37), D55 (= D57), V56 (≠ I58), S72 (≠ C74), P73 (= P75), T81 (= T91), T105 (= T115), T131 (= T174)

6zldA Crystal structure of udp-glucuronic acid 4-epimerase from bacillus cereus in complex with udp-glucuronic acid and NAD (see paper)
31% identity, 91% coverage: 2:298/325 of query aligns to 1:304/314 of 6zldA

query
sites
6zldA

M

M

R

K

I

I

L

L

V

V

T

T

G
|
G

G

A

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

H

H

L

L

I

C

D

Q

R

A

L

L

M

L

-

K

S

N

A

S

G

A

H

Y

E

H

V

V

I

V

C

G

L

I

D
|
D

N
x
H

Y
x
F

F
x
I

-

G

-

P

-

T

-

P

-

A

T

T

G

L

R
x
K

K

T

H

G

N

N

V

I

A

Q

Q

S

W

L

Y

E

G

L

H

N

P

S

R

R

F

F

E

Q

L

F

I

I

R

R

H

E

D
|
D

I
|
I

-

L

-

N

T

T

D

D

P

L

I

S

R

K

L

L

-

L

-

Q

E

D

V

I

D

D

Q

V

I

V

Y

Y

H

H

L
|
L

A
|
A

C
x
A

-

I

P
|
P

A

G

S

V

P
x
R

V

T

H

S

Y

W

Q

G

Y

K

N

D

P

F

I

Q

K

P

T

Y

A

V

K

T

T

N

S

N

F

I

L

M

G

V

T

T

V

Q

N

Q

M

L

L

L

G

E

L

A

A

C

K

K

R

H

V

I

K

K

A

L

-

D

R

K

L

F

L

I

M

H

A
x
I

S

S

T
|
T

S
|
S

E
x
S

V

V

Y

Y

G

G

D

E

P

K

H

S

V

-

H

G

P

A

Q

V

T

S

E

E

D

D

Y

L

W

L

G

-

N

-

V

-

N

-

P

P

I

I

G

P

I

L

R

-

S

S

C

P

Y
|
Y

D

G

E

V

G

T

K
|
K

R

L

V

S

A

G

E

E

T

H

L

L

C

C

F

H

D

V

Y

Y

H

H

R

K

Q

N

H

F

N

H

L

I

E

P

I

I

R

V

V

I

A

L

R

R

I
x
Y

F
|
F

N
x
T

T

V

Y

Y

G

G

P

P

R

R

M

-

L

-

E

Q

N
x
R

D

P

G

D

R
x
M

V
x
A

V

F

S

H

N
x
R

F

L

I

I

V

K

Q

Q

A

M

L

L

Q

E

G

D

Q

K

P

P

L

L

T
|
T

V

I

Y
x
F

G

G

R

D

G

G

E

T

Q
|
Q

T

T

R
|
R

S

D

F

F

C

T

Y

Y

V

I

S

D

D

D

L

C

V

I

D

R

G

G

L

T

I

V

R

A

L

A

M

L

N

E

G

T

D

K

H

K

-

N

-

I

L

I

G

G

P

E

V

V

-

I

N

N

L

I

G

G

N

G

P

K

S

E

E

Q

Y

A

T

S

I
|
I

L

L

Q

D

L

I

A

I

E

S

L

M

I

L

R

E

D

K

R

I

I

S

D

G

P

K

A

S

L

A

P

T

I

K

E

N

F

F

R

L

P

K

L

S

P

V

Q

P

D

G

D
x
E

P

P

Q

K

Q

Q

R

T

R

W

P

A

D

D

I

I

S

S

R

K

A

A

Q

S

A

T

W

L

L

L

K

Q

W

Y

Q

S

P

P

L

T

V

V

S

S

V

L

Q

S

D

D

G

G

L

L

D

E

active site: T126 (= T115), S127 (= S116), S128 (≠ E117), Y149 (= Y144), K153 (= K148)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (= F12), I12 (= I13), D32 (= D32), H33 (≠ N33), F34 (≠ Y34), I35 (≠ F35), K43 (≠ R38), D62 (= D57), I63 (= I58), L81 (= L72), A82 (= A73), A83 (≠ C74), I124 (≠ A113), T126 (= T115), K153 (= K148), Y176 (≠ I171), T178 (≠ N173), R185 (≠ N182), M188 (≠ R185)
binding uridine-5'-diphosphate-glucuronic acid: P85 (= P75), R88 (≠ P78), T126 (= T115), S127 (= S116), S128 (≠ E117), Y149 (= Y144), F177 (= F172), T178 (≠ N173), R185 (≠ N182), M188 (≠ R185), A189 (≠ V186), R192 (≠ N189), T204 (= T201), F206 (≠ Y203), Q211 (= Q208), R213 (= R210), I250 (= I244), E276 (≠ D270)

6zl6A Crystal structure of udp-glucuronic acid 4-epimerase from bacillus cereus in complex with udp and NAD (see paper)
31% identity, 91% coverage: 2:298/325 of query aligns to 1:304/314 of 6zl6A

query
sites
6zl6A

M

M

R

K

I

I

L

L

V

V

T

T

G
|
G

G

A

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

H

H

L

L

I

C

D

Q

R

A

L

L

M

L

-

K

S

N

A

S

G

A

H

Y

E

H

V

V

I

V

C

G

L

I

D
|
D

N
x
H

Y
x
F

F
x
I

-

G

-

P

-

T

-

P

-

A

T

T

G

L

R
x
K

K

T

H

G

N

N

V

I

A

Q

Q

S

W

L

Y

E

G

L

H

N

P

S

R

R

F

F

E

Q

L

F

I

I

R

R

H

E

D
|
D

I
|
I

-

L

-

N

T

T

D

D

P

L

I

S

R

K

L

L

-

L

-

Q

E

D

V

I

D

D

Q

V

I

V

Y

Y

H

H

L
|
L

A
|
A

C
x
A

-

I

P

P

A

G

S

V

P

R

V

T

H

S

Y

W

Q

G

Y

K

N

D

P

F

I

Q

K

P

T

Y

A

V

K

T

T

N

S

N

F

I

L

M

G

V

T

T

V

Q

N

Q

M

L

L

L

G

E

L

A

A

C

K

K

R

H

V

I

K

K

A

L

-

D

R

K

L

F

L

I

M

H

A
x
I

S

S

T
|
T

S
|
S

E
x
S

V

V

Y

Y

G

G

D

E

P

K

H

S

V

-

H

G

P

A

Q

V

T

S

E

E

D

D

Y

L

W

L

G

-

N

-

V

-

N

-

P

P

I

I

G

P

I

L

R

-

S

S

C

P

Y
|
Y

D

G

E

V

G

T

K
|
K

R

L

V

S

A

G

E

E

T

H

L

L

C

C

F

H

D

V

Y

Y

H

H

R

K

Q

N

H

F

N

H

L

I

E

P

I

I

R

V

V

I

A

L

R

R

I
x
Y

F

F

N
x
T

T
x
V

Y

Y

G

G

P

P

R

R

M

-

L

-

E

Q

N
x
R

D

P

G

D

R
x
M

V
x
A

V

F

S

H

N
x
R

F

L

I

I

V

K

Q

Q

A

M

L

L

Q

E

G

D

Q

K

P

P

L

L

T
|
T

V

I

Y
x
F

G

G

R

D

G

G

E

T

Q
|
Q

T

T

R
|
R

S

D

F

F

C

T

Y

Y

V

I

S

D

D

D

L

C

V

I

D

R

G

G

L

T

I

V

R

A

L

A

M

L

N

E

G

T

D

K

H

K

-

N

-

I

L

I

G

G

P

E

V

V

-

I

N

N

L

I

G

G

N

G

P

K

S

E

E

Q

Y

A

T

S

I
|
I

L

L

Q

D

L

I

A

I

E

S

L

M

I

L

R

E

D

K

R

I

I

S

D

G

P

K

A

S

L

A

P

T

I

K

E

N

F

F

R

L

P

K

L

S

P

V

Q

P

D

G

D
x
E

P

P

Q

K

Q

Q

R

T

R

W

P

A

D

D

I

I

S

S

R

K

A

A

Q

S

A

T

W

L

L

L

K

Q

W

Y

Q

S

P

P

L

T

V

V

S

S

V

L

Q

S

D

D

G

G

L

L

D

E

active site: T126 (= T115), S127 (= S116), S128 (≠ E117), Y149 (= Y144), K153 (= K148)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (= F12), I12 (= I13), D32 (= D32), H33 (≠ N33), F34 (≠ Y34), I35 (≠ F35), K43 (≠ R38), D62 (= D57), I63 (= I58), L81 (= L72), A82 (= A73), A83 (≠ C74), I124 (≠ A113), T126 (= T115), K153 (= K148), Y176 (≠ I171), T178 (≠ N173), V179 (≠ T174), R185 (≠ N182), M188 (≠ R185)
binding uridine-5'-diphosphate: T178 (≠ N173), A189 (≠ V186), R192 (≠ N189), T204 (= T201), F206 (≠ Y203), Q211 (= Q208), R213 (= R210), I250 (= I244), E276 (≠ D270)

6zllA Crystal structure of udp-glucuronic acid 4-epimerase from bacillus cereus in complex with udp-galacturonic acid and NAD (see paper)
31% identity, 91% coverage: 2:298/325 of query aligns to 1:304/321 of 6zllA

query
sites
6zllA

M

M

R

K

I

I

L

L

V

V

T

T

G
|
G

G

A

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

H

H

L

L

I

C

D

Q

R

A

L

L

M

L

-

K

S

N

A

S

G

A

H

Y

E

H

V

V

I

V

C

G

L

I

D
|
D

N
x
H

Y
x
F

F
x
I

-

G

-

P

-

T

-

P

-

A

T

T

G

L

R
x
K

K

T

H

G

N

N

V

I

A

Q

Q

S

W

L

Y

E

G

L

H

N

P

S

R

R

F

F

E

Q

L

F

I

I

R

R

H

E

D
|
D

I
|
I

-

L

-

N

T

T

D

D

P

L

I

S

R

K

L

L

-

L

-

Q

E

D

V

I

D

D

Q

V

I

V

Y

Y

H

H

L
|
L

A
|
A

C
x
A

-

I

P
|
P

A

G

S
x
V

P
x
R

V

T

H

S

Y

W

Q

G

Y

K

N

D

P

F

I

Q

K

P

T

Y

A

V

K

T

T

N

S

N

F

I

L

M

G

V

T

T

V

Q

N

Q

M

L

L

L

G

E

L

A

A

C

K

K

R

H

V

I

K

K

A

L

-

D

R

K

L

F

L

I

M

H

A
x
I

S

S

T
|
T

S
|
S

E
x
S

V

V

Y

Y

G

G

D

E

P

K

H

S

V

-

H

G

P

A

Q

V

T

S

E

E

D

D

Y

L

W

L

G

-

N

-

V

-

N

-

P

P

I

I

G

P

I

L

R

-

S

S

C

P

Y
|
Y

D

G

E

V

G

T

K
|
K

R

L

V

S

A

G

E

E

T

H

L

L

C

C

F

H

D

V

Y

Y

H

H

R

K

Q

N

H

F

N

H

L

I

E

P

I

I

R

V

V

I

A

L

R

R

I
x
Y

F

F

N
x
T

T
x
V

Y

Y

G

G

P

P

R

R

M

-

L

-

E

Q

N
x
R

D

P

G

D

R
x
M

V
x
A

V

F

S

H

N
x
R

F

L

I

I

V

K

Q

Q

A

M

L

L

Q

E

G

D

Q

K

P

P

L

L

T
|
T

V

I

Y
x
F

G

G

R

D

G

G

E

T

Q
|
Q

T

T

R
|
R

S

D

F

F

C

T

Y

Y

V

I

S

D

D

D

L

C

V

I

D

R

G

G

L

T

I

V

R

A

L

A

M

L

N

E

G

T

D

K

H

K

-

N

-

I

L

I

G

G

P

E

V

V

-

I

N

N

L

I

G

G

N

G

P

K

S

E

E

Q

Y

A

T

S

I
|
I

L

L

Q

D

L

I

A

I

E

S

L

M

I

L

R

E

D

K

R

I

I

S

D

G

P

K

A

S

L

A

P

T

I

K

E

N

F

F

R

L

P

K

L

S

P

V

Q

P

D

G

D
x
E

P

P

Q

K

Q

Q

R

T

R

W

P

A

D

D

I

I

S

S

R

K

A

A

Q

S

A

T

W

L

L

L

K

Q

W

Y

Q

S

P

P

L

T

V

V

S

S

V

L

Q

S

D

D

G

G

L

L

D

E

active site: T126 (= T115), S127 (= S116), S128 (≠ E117), Y149 (= Y144), K153 (= K148)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (= F12), I12 (= I13), D32 (= D32), H33 (≠ N33), F34 (≠ Y34), I35 (≠ F35), K43 (≠ R38), D62 (= D57), I63 (= I58), L81 (= L72), A82 (= A73), A83 (≠ C74), I124 (≠ A113), T126 (= T115), Y149 (= Y144), K153 (= K148), Y176 (≠ I171), V179 (≠ T174), R185 (≠ N182), M188 (≠ R185)
binding (2S,3R,4S,5R,6R)-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4,5-trihydroxy-oxane-2-carboxylic acid: P85 (= P75), V87 (≠ S77), R88 (≠ P78), T126 (= T115), S127 (= S116), Y149 (= Y144), T178 (≠ N173), R185 (≠ N182), A189 (≠ V186), R192 (≠ N189), T204 (= T201), F206 (≠ Y203), Q211 (= Q208), R213 (= R210), I250 (= I244), E276 (≠ D270)

6zljA Crystal structure of udp-glucuronic acid 4-epimerase y149f mutant from bacillus cereus in complex with udp-4-deoxy-4-fluoro-glucuronic acid and NAD (see paper)
30% identity, 91% coverage: 2:298/325 of query aligns to 1:304/314 of 6zljA

query
sites
6zljA

M

M

R

K

I

I

L

L

V

V

T

T

G
|
G

G

A

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

H

H

L

L

I

C

D

Q

R

A

L

L

M

L

-

K

S

N

A

S

G

A

H

Y

E

H

V

V

I

V

C

G

L

I

D
|
D

N
x
H

Y
x
F

F
x
I

-

G

-

P

-

T

-

P

-

A

T

T

G

L

R
x
K

K

T

H

G

N

N

V

I

A

Q

Q

S

W

L

Y

E

G

L

H

N

P

S

R

R

F

F

E

Q

L

F

I

I

R

R

H

E

D
|
D

I
|
I

-

L

-

N

T

T

D

D

P

L

I

S

R

K

L

L

-

L

-

Q

E

D

V

I

D

D

Q

V

I

V

Y

Y

H

H

L
|
L

A
|
A

C
x
A

-

I

P
|
P

A

G

S

V

P
x
R

V

T

H

S

Y

W

Q

G

Y

K

N

D

P

F

I

Q

K

P

T

Y

A

V

K

T

T

N

S

N

F

I

L

M

G

V

T

T

V

Q

N

Q

M

L

L

L

G

E

L

A

A

C

K

K

R

H

V

I

K

K

A

L

-

D

R

K

L

F

L

I

M

H

A
x
I

S

S

T
|
T

S
|
S

E
x
S

V

V

Y

Y

G

G

D

E

P

K

H

S

V

G

H

A

P

V

Q

S

T

E

E

D

D

L

Y

L

W

P

G

I

N

P

V

L

N

S

P

P

I
x
F

G

G

I

V

R

T

S

-

C

-

Y

-

D

-

E

-

G

-

K
|
K

R

L

V

S

A

G

E

E

T

H

L

L

C

C

F

H

D

V

Y

Y

H

H

R

K

Q

N

H

F

N

H

L

I

E

P

I

I

R

V

V

I

A

L

R

R

I
x
Y

F
|
F

N
x
T

T
x
V

Y

Y

G

G

P

P

R

R

M

-

L

-

E

Q

N
x
R

D

P

G

D

R
x
M

V
x
A

V

F

S

H

N
x
R

F

L

I

I

V

K

Q

Q

A

M

L

L

Q

E

G

D

Q

K

P

P

L

L

T
|
T

V

I

Y
x
F

G

G

R

D

G

G

E

T

Q
|
Q

T

T

R
|
R

S

D

F

F

C

T

Y

Y

V

I

S

D

D

D

L

C

V

I

D

R

G

G

L

T

I

V

R

A

L

A

M

L

N

E

G

T

D

K

H

K

-

N

-

I

L

I

G

G

P

E

V

V

-

I

N

N

L

I

G

G

N

G

P

K

S

E

E

Q

Y

A

T

S

I
|
I

L

L

Q

D

L

I

A

I

E

S

L

M

I

L

R

E

D

K

R

I

I

S

D

G

P

K

A

S

L

A

P

T

I

K

E

N

F

F

R

L

P

K

L

S

P

V

Q

P

D

G

D
x
E

P

P

Q

K

Q

Q

R

T

R

W

P

A

D

D

I

I

S

S

R

K

A

A

Q

S

A

T

W

L

L

L

K

Q

W

Y

Q

S

P

P

L

T

V

V

S

S

V

L

Q

S

D

D

G

G

L

L

D

E

active site: T126 (= T115), S127 (= S116), S128 (≠ E117), F149 (≠ I138), K153 (= K148)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (= F12), I12 (= I13), D32 (= D32), H33 (≠ N33), F34 (≠ Y34), I35 (≠ F35), K43 (≠ R38), D62 (= D57), I63 (= I58), L81 (= L72), A82 (= A73), A83 (≠ C74), I124 (≠ A113), T126 (= T115), K153 (= K148), Y176 (≠ I171), T178 (≠ N173), V179 (≠ T174), R185 (≠ N182), M188 (≠ R185)
binding (2~{R},3~{S},4~{R},5~{R},6~{R})-6-[[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3-fluoranyl-4,5-bis(oxidanyl)oxane-2-carboxylic acid: P85 (= P75), R88 (≠ P78), T126 (= T115), S127 (= S116), S128 (≠ E117), F149 (≠ I138), F177 (= F172), T178 (≠ N173), R185 (≠ N182), M188 (≠ R185), A189 (≠ V186), R192 (≠ N189), T204 (= T201), F206 (≠ Y203), Q211 (= Q208), R213 (= R210), I250 (= I244), E276 (≠ D270)

6bwlA X-ray structure of pal from bacillus thuringiensis (see paper)
33% identity, 92% coverage: 2:300/325 of query aligns to 1:305/313 of 6bwlA

query
sites
6bwlA

M

M

R

K

I

I

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

F
|
F

I
|
I

G

G

S

R

H

W

L

V

I

V

D

K

R

R

L

L

M

L

S

Q

A

D

G

K

H

H

E

E

V

V

I

W

C

I

L

L

D
|
D

N
|
N

Y
x
L

F

A

T
x
N

G
x
S

R

T

K

T

H

A

N

N

V

I

A

T

Q

E

W

F

Y

A

G

H

H

D

P

L

R

N

F

L

-

K

E

Q

L

C

I

I

R

Q

H

G

D
|
D

I
|
I

T

K

D

D

P

K

I

K

R

L

L

V

-

A

-

Q

-

L

-

F

-

E

-

N

E

S

V

F

D

D

Q

L

I

C

Y

Y

H

H

L
|
L

A

-

C

-

P

-

A
|
A

S
x
A

P

S

V
x
I

H

N

Y

V

Q

Q

Y

-

N

D

P

S

I

I

K

D

T

D

A

A

K

R

T

A

S

T

F

F

-

E

-

N

-

D

-

T

L

I

G

G

T

T

V

F

N

N

M

L

L

L

G

E

L

Q

A

C

K

L

R

N

V

Y

K

D

A

V

R

K

L

M

L

V

M

F

A
x
M

S

S

T
|
T

S
x
C

E
x
M

V

V

Y

Y

G

-

D

D

P

K

H

A

V

T

H

N

P

I

Q

Q

T

G

E

-

D

-

Y

-

W

I

G

S

N

E

V

L

N

D

P

P

I

I

G

K

I

P

R

A

S

S

C

P

Y
|
Y

D

A

E

G

G

S

K
|
K

R

I

V

A

A

A

E

E

T

N

L

M

C

V

F

L

D

S

Y

Y

H

Y

R

Y

Q

A

H

Y

N

K

L

L

E

P

I

V

R

V

V

V

A

I

R

R

I

P

F

F

N
|
N

T
|
T

Y

Y

G

G

P

P

-

F

R

Q

M

K

L

T

E

G

N

G

D

E

G

G

R
x
G

V
|
V

V

V

S

A

N

I

F

F

I

I

V

N

Q

N

A

K

L

L

Q

D

G

N

Q

V

P

P

L

L

T
x
N

V
x
I

Y
|
Y

G

G

R

D

G

G

E

K

Q
|
Q

T

T

R
|
R

S

D

F

L

C

L

Y

Y

V

V

S

E

D

D

L

C

V

A

D

D

-

F

-

V

-

A

G

G

L

Y

I

S

R

A

L

K

M

A

N

N

G

G

D

-

H

H

L

I

G

-

P

-

V

I

N

N

L

A

G

G

N

T

P

G

S

Q

E

D

Y

I

T

S

I
|
I

L

N

Q

K

L

L

A

A

E

E

L

L

I

I

-

S

-

G

-

N

R

K

D

V

R

S

I

I

D

Q

P

H

A

V

L

T

P

H

I

I

E

H

F

-

R

-

P

-

L

-

P

P

Q

Q

D

S

D
x
E

P

I

Q

Q

Q

K

R

L

P

C

D

N

I

Y

S

E

R

K

A

A

Q

K

A

T

W

I

L

L

K

N

W

W

Q

E

P

P

L

K

V

V

S

S

V

L

Q

E

D

D

G

G

L

V

D

I

R

K

T

T

active site: T122 (= T115), C123 (≠ S116), M124 (≠ E117), Y147 (= Y144), K151 (= K148)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (= F12), I12 (= I13), D31 (= D32), N32 (= N33), L33 (≠ Y34), N35 (≠ T36), S36 (≠ G37), D57 (= D57), I58 (= I58), L79 (= L72), A80 (= A76), A81 (≠ S77), I83 (≠ V79), M120 (≠ A113), K151 (= K148), N176 (= N173), T177 (= T174)
binding uridine-5'-diphosphate: N176 (= N173), G189 (≠ R185), V190 (= V186), N205 (≠ T201), I206 (≠ V202), Y207 (= Y203), Q212 (= Q208), R214 (= R210), I250 (= I244), E275 (≠ D270)

4zrnA Crystal structure of udp-glucose 4-epimerase (tm0509) with udp-glucose from hyperthermophilic eubacterium thermotoga maritima (see paper)
30% identity, 94% coverage: 2:305/325 of query aligns to 1:305/309 of 4zrnA

query
sites
4zrnA

M

M

R

N

I

I

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

H

H

L

V

I

V

D

D

R

K

L

L

M

I

S

E

A

N

G

G

H

Y

E

G

V

V

I

I

C

V

L

V

D
|
D

N
|
N

Y

L

F
x
S

T
x
S

G
|
G

R

K

K

V

H

E

N

N

V

L

-

N

-

R

-

N

A

A

Q

L

W

F

Y

Y

G

-

H

-

P

-

R

-

F

-

E

-

L

-

I

-

R

E

H

Q

D
x
S

I
|
I

T

E

D

D

P

E

I

E

R

M

L

M

E

E

V

R

-

I

-

F

-

S

-

L

-

H

-

R

-

P

D

E

Q

Y

I

V

Y

F

H

H

L
|
L

A
|
A

C
x
A

P

Q

A

A

S

S

P

V

V

A

H

I

Y

S

Q

V

Y

R

N

E

P

P

I

A

K

R

T

D

A

A

K

K

T
|
T

S

N

F

I

L

I

G

G

T

S

V

L

N

V

M

L

L

L

-

E

-

K

G

S

L

I

A

K

K

Y

R

G

V

V

K

K

A

K

R

F

L

I

L

F

M
x
S

A
x
S

S
x
T

T

G

S
x
G

E
x
A

V

I

Y

Y

G

G

D

E

P

N

-

V

H

K

V

V

H

F

P

P

Q

T

T

P

E

E

D

T

Y

E

W

I

G

P

N

H

-

P

V

I

N

S

P

P

I
x
Y

G

G

I

I

R

-

S

-

C

-

Y

-

D

-

E

-

G

A

K
|
K

R

Y

V

S

A

T

E

E

T

M

L

Y

C

L

F

E

D

F

Y

F

H

A

R

R

Q

E

H

Y

N

G

L

L

E

K

I

Y

R

T

V

V

A

L

R

R

I
x
Y

F

A

N
|
N

T
x
V

Y

Y

G

G

P

P

R

R

M

Q

-

D

L

P

E
x
Y

N

G

D

E

G

A

R
x
G

V
|
V

V

V

S

A

N

I

F

F

I

T

V

E

Q

R

A

M

L

L

Q

R

G

G

Q

E

P

E

L

V

T
x
H

V

I

Y
x
F

G

G

R

D

G

G

E

E

Q
x
Y

T

V

R
|
R

S

D

F

Y

C

V

Y

Y

V

V

S

D

D

D

L

V

V

V

D

R

G

A

-

N

L

L

I

L

R

A

L

M

M

E

N

K

G

G

D

D

H

N

L

-

G

E

P

V

V

F

N

N

L

I

G

G

N

T

P

G

S

R

E

G

Y

T

T

T

I
x
V

L

N

Q

Q

L

L

A

F

E

K

L

L

I

L

R

K

D

E

R

I

I

T

D

G

P

Y

A

D

L

K

P

E

I

P

E

V

F

Y

R

K

P

P

L

P

P
x
R

Q

K

D

G

D
|
D

P

V

Q

R

Q

K

R

S

R

I

P

L

D

D

I

Y

S

T

R

K

A

A

Q

K

A

E

W

K

L

L

K

G

W

W

Q

E

P

P

L

K

V

V

S

S

V

L

Q

E

D

E

G

G

L

L

D

K

R

L

T

T

I

V

A

E

D

Y

F

F

R

R

active site: T117 (≠ S114), G119 (≠ S116), A120 (≠ E117), Y143 (≠ I138), K147 (= K148), Y181 (≠ E181), G185 (≠ R185)
binding nicotinamide-adenine-dinucleotide: G7 (= G8), G10 (= G11), F11 (= F12), I12 (= I13), D31 (= D32), N32 (= N33), S34 (≠ F35), S35 (≠ T36), G36 (= G37), S51 (≠ D57), I52 (= I58), L73 (= L72), A74 (= A73), A75 (≠ C74), T92 (= T91), S115 (≠ M112), S116 (≠ A113), Y143 (≠ I138), K147 (= K148), Y170 (≠ I171), V173 (≠ T174)
binding uridine-5'-diphosphate-glucose: T117 (≠ S114), G119 (≠ S116), A120 (≠ E117), Y143 (≠ I138), N172 (= N173), G185 (≠ R185), V186 (= V186), H201 (≠ T201), F203 (≠ Y203), Y208 (≠ Q208), R210 (= R210), V244 (≠ I244), R267 (≠ P267), D270 (= D270)

6pnlA Structure of epimerase mth375 from the thermophilic pseudomurein- containing methanogen methanothermobacter thermautotrophicus
30% identity, 91% coverage: 4:300/325 of query aligns to 18:319/336 of 6pnlA

query
sites
6pnlA

I

V

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

F
x
C

I

V

G

G

S

S

H

N

L

L

I

T

D

G

R

N

L

L

M

A

S

K

A

A

G

G

H

A

E

N

V

V

I

I

C

I

L

L

D
|
D

N
|
N

Y
x
L

F
x
S

T
x
S

G
x
S

R

Y

K

E

H

W

N

N

V

I

A

P

Q

E

W

-

Y

-

G

-

H

Y

P

E

R

N

F

I

E

E

L

F

I

V

R

K

H

G

D
|
D

I
|
I

T

L

D

D

P

D

I

E

R

V

L

L

E

K

V

R

-

V

-

F

-

K

-

E

-

R

-

P

D

D

Q

Y

I

V

Y

F

H

H

L
|
L

A

A

C
x
A

P

H

A
x
F

S

A

P

N

V

Q

H

N

Y

S

Q

V

Y

D

N

N

P

P

I

E

K

K

T

D

A

L

K

L

T
x
V

S

N

F

G

L

L

G

G

T

I

V

L

N

K

M

V

L

L

G

E

L

Y

A

A

K

Q

R

L

V

V

K

G

A

V

R

E

L

R

L

F

M

V

A

Y

S
|
S

T

S

S
|
S

-

G

-
x
C

E
x
G

V

V

Y

Y

G

G

-

L

D

D

P

S

H

K

V

I

H

P

P

F

Q

E

T

E

E

H

D

D

Y

I

W

-

G

-

N

-

V

-

N

-

P

S

I

I

G

S

I

L

R

H

S

T

C

P

Y
|
Y

D

Q

E

V

G

T

K
|
K

R

L

V

L

A

G

E

E

T

L

L

Y

C

T

F

N

D

Y

Y

F

H

H

R

N

Q

L

H

Y

N

E

L

M

E

P

I

I

R

V

V

N

A

A

R

R

I
x
F

F

F

N
|
N

T
x
V

Y

F

G

G

P

P

R

G

M

E

L

V

E

P

N

G

D

K

G

Y

R
|
R

-
x
N

V
|
V

V

I

S

P

N

N

F
|
F

I

F

V

Y

Q

W

A

A

L

M

Q

N

G

Q

Q

Q

P

P

L

L

T
x
P

V
x
I

Y
x
T

G

G

R

D

G

G

E

S

Q

E

T

T

R
|
R

S

D

F

W

C

T

Y

F

V

V

S

E

D

D

L

I

V

V

D

R

G

G

L

L

I

M

R

A

L

M

-

G

M

V

N

R

G

R

D

E

H

A

L

I

G

G

-

E

P

A

V

I

N

N

L

L

G

G

N

S

P

G

S

T

E

E

Y

H

T

Q

I
x
V

L

I

Q

E

L

M

A

A

E

G

L

I

I

I

R

N

D

E

R

L

I

T

D

E

P

N

A

P

L

A

P

G

I

V

E

V

F

Y

R

R

P

P

L

R

P
x
R

Q

D

D

W

D

D

P

A

Q

K

Q

T

R

R

-

L

R

L

P

S

D

S

I

I

S

D

R

K

A

A

Q

R

A

R

W

L

L

L

K

D

W

Y

Q

E

P

P

L

Q

V

V

S

S

V

F

Q

R

D

E

G

G

L

L

D

E

R

R

T

T

active site: S133 (= S116), C135 (vs. gap), G136 (≠ E117), Y159 (= Y144), K163 (= K148)
binding nicotinamide-adenine-dinucleotide: G22 (= G8), G25 (= G11), C26 (≠ F12), D46 (= D32), N47 (= N33), L48 (≠ Y34), S49 (≠ F35), S50 (≠ T36), S51 (≠ G37), D68 (= D57), I69 (= I58), L89 (= L72), A91 (≠ C74), F93 (≠ A76), V108 (≠ T91), S131 (= S114), S133 (= S116), Y159 (= Y144), K163 (= K148), F186 (≠ I171), N188 (= N173), V189 (≠ T174), R200 (= R185)
binding uridine-5'-diphosphate: N188 (= N173), N201 (vs. gap), V202 (= V186), F206 (= F190), P217 (≠ T201), I218 (≠ V202), T219 (≠ Y203), R226 (= R210), V262 (≠ I244), R285 (≠ P267)

6pmhA Structure of epimerase mth375 from the thermophilic pseudomurein- containing methanogen methanothermobacter thermautotrophicus
30% identity, 91% coverage: 4:300/325 of query aligns to 12:313/330 of 6pmhA

query
sites
6pmhA

I

V

L

L

V

V

T

T

G
|
G

G

G

A

A

G
|
G

F

C

I

V

G

G

S

S

H

N

L

L

I

T

D

G

R

N

L

L

M

A

S

K

A

A

G

G

H

A

E

N

V

V

I

I

C

I

L

L

D
|
D

N
|
N

Y

L

F
x
S

T
x
S

G
x
S

R

Y

K

E

H

W

N

N

V

I

A

P

Q

E

W

-

Y

-

G

-

H

Y

P

E

R

N

F

I

E

E

L

F

I

V

R

K

H

G

D
|
D

I
|
I

T

L

D

D

P

D

I

E

R

V

L

L

E

K

V

R

-

V

-

F

-

K

-

E

-

R

-

P

D

D

Q

Y

I

V

Y

F

H

H

L

L

A
|
A

C

A

P

H

A
x
F

S

A

P

N

V

Q

H

N

Y

S

Q

V

Y

D

N

N

P

P

I

E

K

K

T

D

A

L

K

L

T

V

S

N

F

G

L

L

G

G

T

I

V

L

N

K

M

V

L

L

G

E

L

Y

A

A

K

Q

R

L

V

V

K

G

A

V

R

E

L

R

L

F

M

V

A

Y

S

S

T

S

S
|
S

-

G

-
x
C

E
x
G

V

V

Y

Y

G

G

-

L

D

D

P

S

H

K

V

I

H

P

P

F

Q

E

T

E

E

H

D

D

Y

I

W

-

G

-

N

-

V

-

N

-

P

S

I

I

G

S

I

L

R

H

S

T

C

P

Y
|
Y

D

Q

E

V

G

T

K
|
K

R

L

V

L

A

G

E

E

T

L

L

Y

C

T

F

N

D

Y

Y

F

H

H

R

N

Q

L

H

Y

N

E

L

M

E

P

I

I

R

V

V

N

A

A

R

R

I

F

F

F

N
|
N

T

V

Y

F

G

G

P

P

R

G

M

E

L

V

E

P

N

G

D

K

G

Y

R
|
R

-
x
N

V
|
V

V

I

S

P

N

N

F
|
F

I

F

V

Y

Q

W

A

A

L

M

Q

N

G

Q

Q

Q

P

P

L

L

T
x
P

V
x
I

Y
x
T

G

G

R

D

G

G

E

S

Q

E

T

T

R
|
R

S

D

F

W

C

T

Y

F

V

V

S

E

D

D

L

I

V

V

D

R

G

G

L

L

I

M

R

A

L

M

-

G

M

V

N

R

G

R

D

E

H

A

L

I

G

G

-

E

P

A

V

I

N

N

L

L

G

G

N

S

P

G

S

T

E

E

Y

H

T

Q

I
x
V

L

I

Q

E

L

M

A

A

E

G

L

I

I

I

R

N

D

E

R

L

I

T

D

E

P

N

A

P

L

A

P

G

I

V

E

V

F

Y

R

R

P

P

L

R

P
x
R

Q

D

D

W

D

D

P

A

Q

K

Q

T

R

R

-

L

R

L

P

S

D

S

I

I

S

D

R

K

A

A

Q

R

A

R

W

L

L

L

K

D

W

Y

Q

E

P

P

L

Q

V

V

S

S

V

F

Q

R

D

E

G

G

L

L

D

E

R

R

T

T

active site: S127 (= S116), C129 (vs. gap), G130 (≠ E117), Y153 (= Y144), K157 (= K148)
binding adenosine monophosphate: G16 (= G8), G19 (= G11), D40 (= D32), N41 (= N33), S43 (≠ F35), S44 (≠ T36), S45 (≠ G37), D62 (= D57), I63 (= I58), A84 (= A73), F87 (≠ A76), R194 (= R185)
binding uridine-5'-diphosphate: C129 (vs. gap), N182 (= N173), N195 (vs. gap), V196 (= V186), F200 (= F190), P211 (≠ T201), I212 (≠ V202), T213 (≠ Y203), R220 (= R210), V256 (≠ I244), R279 (≠ P267)

6h0pA The structure of c100a mutant of arabidopsis thaliana udp-apiose/udp- xylose synthase in complex with nadh and udp-d-glucuronic acid (see paper)
28% identity, 96% coverage: 2:313/325 of query aligns to 11:372/375 of 6h0pA

query
sites
6h0pA

M

L

R

T

I

I

L

C

V

M

T

I

G
|
G

G

A

A

G

G
|
G

F
|
F

I
|
I

G

G

S

S

H

H

L

L

I

C

D

E

R

K

L

L

M

L

S

T

A

E

G

T

-

P

H

H

E

K

V

V

I

L

C

A

L

L

D
|
D

N

V

Y

Y

F

N

T

D

G

K

R

I

K

K

H

H

-

L

-

E

-

P

N

D

V

T

A

V

Q

E

W

W

Y

S

G

G

H

-

P

-

R

R

F

I

E

Q

L

F

I

H

R

R

H

I

D
x
N

I
|
I

T

K

D

H

P

D

I

S

R

R

L

L

E

E

-

G

-

L

-

V

-

K

-

M

V

A

D

D

Q

L

I

I

Y

I

H

N

L
|
L

A
|
A

C
x
A

P

I

A
|
A

S

T

P
|
P

V

A

H

D

Y
|
Y

Q

N

Y

T

N

R

P

P

I

L

K

D

T

T

A

I

K

Y

T

S

S

N

F

F

L

I

G

D

T

A

V

L

N

P

M

V

L

V

G

K

L

Y

A

C

K

S

R

E

V

N

K

N

A

K

R

R

L

L

L

I

M

H

A
x
F

S

S

T
|
T

S

C

E
|
E

V

V

Y

Y

G

G

D

K

-

T

-

I

-

G

-

S

-

F

-

L

P

P

H

K

V

D

H

H

P

P

Q

L

T

R

E

D

D

D

Y

P

W

A

G

F

N

Y

V

V

N

L

P

K

I

E

G

D

I

I

R

S

S

P

C

C

-

I

-

F

-

G

-

S

-

I

-

E

-

K

-

Q

-
x
R

-

W

-

S

Y
|
Y

D

A

E

C

G

A

K
|
K

R

Q

V

L

A

I

E

E

T

R

L

L

C

V

F

Y

D

A

Y

E

H

G

R

A

Q

E

H

N

N

G

L

L

E

E

I

F

R

T

V

I

A

V

R

R

I

P

F

F

N
|
N

T

W

Y

I

G

G

P

P

R

R

M

M

-

D

-

F

-

I

-

P

-

G

-

I

-

D

-

G

-

P

L

S

E

E

N

G

D

V

G

P

R
|
R

V
|
V

V

L

S

A

N
x
C

F
|
F

I

S

V

N

Q

N

A

L

L

L

Q

R

G

R

Q

E

P

P

L

L

T

K

V

L

Y
x
V

G

D

R

G

G

G

E

E

Q

S

T

Q

R
|
R

S

T

F

F

C

V

Y

Y

V

I

S

N

D

D

L

A

V

I

D

E

G

A

L

V

I

L

R

L

L

M

M

I

N

E

G

N

D

P

H

E

L

R

G

A

P

N

-

G

-

H

-

I

V

F

N

N

L

V

G

G

N

N

P

P

-

N

S

N

E

E

Y

V

T

T

I
x
V

L

R

Q

Q

L

L

A

A

E

E

L

M

I

M

R

T

D

E

R

V

I

Y

-

A

-

K

-

V

-

S

-

G

-

E

-

G

-

A

-

I

-

E

D

S

P

P

A

T

L

V

P

D

I

V

-

S

-

K

E

E

F
|
F

R
x
Y

P

G

L

E

P

G

Q
x
Y

D

D

D
|
D

P

S

Q

D

Q

K

R
|
R

R

I

P

P

D

D

I

M

S

T

R

I

A

I

Q

N

A

R

W

Q

L

L

K

G

W

W

Q

N

P

P

L

K

V

T

S

S

V

L

Q

W

D

D

G

L

L

L

D

E

R

S

T

T

I

L

A

T

D

-

F

Y

R

Q

D

H

R

R

Q

T

Q

Y

A

A

A

E

A

A

T

V

binding nicotinamide-adenine-dinucleotide: G17 (= G8), G20 (= G11), F21 (= F12), I22 (= I13), D42 (= D32), N69 (≠ D57), I70 (= I58), L89 (= L72), A90 (= A73), A91 (≠ C74), A93 (= A76), F130 (≠ A113), Y178 (= Y144), K182 (= K148)
binding uridine-5'-diphosphate-glucuronic acid: P95 (= P78), Y98 (= Y81), T132 (= T115), E134 (= E117), R175 (vs. gap), Y178 (= Y144), N207 (= N173), R228 (= R185), V229 (= V186), C232 (≠ N189), F233 (= F190), V246 (≠ Y203), R253 (= R210), V291 (≠ I244), F323 (= F263), Y324 (≠ R264), Y328 (≠ Q268), D330 (= D270), R334 (= R274)

8vr2B Crystal structure of the pcryo_0617 oxidoreductase/decarboxylase from psychrobacter cryohalolentis k5 in the presence of NAD and udp
30% identity, 92% coverage: 3:300/325 of query aligns to 9:307/321 of 8vr2B

query
sites
8vr2B

R

K

I

I

L

F

V

I

T

T

G
|
G

G

G

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

H

T

L

L

I

I

D

G

R

R

L

L

M

I

S

E

A

-

G

N

H

N

E

E

V

M

I

I

C

V

L

Y

D
|
D

N
|
N

Y

L

F

-

T

-

G

-

R

-

K
x
E

H
x
R

N

N

V

T

A

L

Q

K

-

S

-

Q

-

P

W

F

Y

A

G

N

H

H

P

K

R

N

F

L

E

T

L

L

I

I

R

Q

H

G

D
x
N

I
x
V

T

L

D

D

P

Q

I

E

R

K

L

I

-

I

-

E

-

A

E

K

V

G

D

S

Q

E

I

I

Y

F

-

I

H

H

L
x
A

A
|
A

C
x
A

P

I

A

A

S

G

P

I

V

D

H

N

Y

T

Q

V

Y

K

N

S

P

P

I

V

K

R

T

T

A

M

K

T

T

V

S

N

F

M

L

I

G

G

T

T

V

A

N

N

M

A

L

L

G

E

L

A

A

A

K

H

R

Q

V

A

K

G

A

T

-

V

-

Q

R

R

L

F

L

L

M

E

A
x
F

S

S

T

T

S

S

E

E

V

V

Y

F

G

G

D

S

P

R

H

A

V

Y

H

R

P

V

Q

D

T

E

E

T

D

G

Y

A

W

V

G

G

N

E

V

A

N

-

P

-

I

-

G

-

I

-

R
|
R

S

W

C

T

Y

Y

D

A

E

V

G

S

K
|
K

R

L

V

A

A

G

E

E

T

H

L

L

C

T

F

H

D

A

Y

Y

H

N

R

R

Q

E

H

H

N

G

L

L

E

P

I

T

R

V

V

T

A

F

R

R

I
x
P

F

F

N
|
N

T

V

Y

Y

G

G

P

P

R

G

M

Q

L

I

E

G

N

E

D
x
G

G
x
A

R

-

V

-

V

I

S

S

N

I

F
x
M

I

I

V

R

Q

K

A

A

L

L

Q

N

G

N

Q

E

P

D

L

I

T
x
Y

V
x
I

Y
x
F

G

G

R

D

G

G

E

S

Q

Q

T

I

R
|
R

S

A

F

W

C

C

Y

Y

V

V

S

D

D

D

L

M

V

I

D

D

G

A

L

L

I

M

R

K

L

A

M

L

N

S

-

V

-

P

-

Q

-

A

-

I

G

G

D

E

H

S

L

F

G

-

P

-

V

-

N

N

L

I

G

G

N

N

P

A

S

R

E

A

Y

I

-

T

T

T

I
|
I

L

Y

Q

G

L

L

A

A

E

Q

L

T

I

I

R

C

D

R

R

V

I

L

D

N

P

S

A

K

L

S

P

E

I

I

E

I

F

F

R

R

P

E

L

A

P

L

Q

S

D

A

D

D

P

I

Q

E

Q

L

R

R

R

I

P

P

D

N

I

V

S

D

R

K

A

S

Q

E

A

E

W

L

L

L

K

G

W

F

Q

K

P

A

L

Q

V

V

S

D

V

L

Q

E

D

E

G

G

L

L

D

I

R

R

T

T

binding nicotinamide-adenine-dinucleotide: G14 (= G8), G17 (= G11), F18 (= F12), I19 (= I13), D37 (= D32), N38 (= N33), E40 (≠ K39), R41 (≠ H40), N61 (≠ D57), V62 (≠ I58), A81 (≠ L72), A82 (= A73), A83 (≠ C74), F124 (≠ A113), K154 (= K148), P177 (≠ I171), N179 (= N173)
binding uridine-5'-diphosphate: R147 (= R141), G189 (≠ D183), A190 (≠ G184), M194 (≠ F190), Y205 (≠ T201), I206 (≠ V202), F207 (≠ Y203), R214 (= R210), I251 (= I244)

6h0nA The structure of wild-type arabidopsis thaliana udp-apiose/udp-xylose synthase in complex with NAD+ and udp (see paper)
28% identity, 96% coverage: 2:313/325 of query aligns to 11:372/377 of 6h0nA

query
sites
6h0nA

M

L

R

T

I

I

L

C

V

M

T

I

G
|
G

G

A

A

G

G
|
G

F
|
F

I
|
I

G

G

S

S

H

H

L

L

I

C

D

E

R

K

L

L

M

L

S

T

A

E

G

T

-

P

H

H

E

K

V

V

I

L

C

A

L

L

D
|
D

N
x
V

Y

Y

F

N

T

D

G

K

R

I

K

K

H

H

-

L

-

E

-

P

N

D

V

T

A

V

Q

E

W

W

Y

S

G

G

H

-

P

-

R

R

F

I

E

Q

L

F

I

H

R

R

H
x
I

D
x
N

I
|
I

T

K

D

H

P

D

I

S

R

R

L

L

E

E

-

G

-

L

-

V

-

K

-

M

V

A

D

D

Q

L

I

I

Y

I

H

N

L
|
L

A
|
A

C
x
A

P

I

A
x
C

S

T

P
|
P

V

A

H

D

Y

Y

Q

N

Y

T

N

R

P

P

I

L

K

D

T

T

A

I

K

Y

T

S

S

N

F

F

L

I

G

D

T

A

V

L

N

P

M

V

L

V

G

K

L

Y

A

C

K

S

R

E

V

N

K

N

A

K

R

R

L

L

L

I

M

H

A

F

S
|
S

T
|
T

S

C

E

E

V

V

Y

Y

G

G

D

K

-

T

-

I

-

G

-

S

-

F

-

L

P

P

H

K

V

D

H

H

P

P

Q

L

T

R

E

D

D

D

Y

P

W

A

G

F

N

Y

V

V

N

L

P

K

I

E

G

D

I

I

R

S

S

P

C

C

-

I

-

F

-

G

-

S

-

I

-

E

-

K

-

Q

-
x
R

-

W

-

S

Y
|
Y

D

A

E

C

G

A

K
|
K

R

Q

V

L

A

I

E

E

T

R

L

L

C

V

F

Y

D

A

Y

E

H

G

R

A

Q

E

H

N

N

G

L

L

E

E

I

F

R

T

V

I

A

V

R

R

I
x
P

F

F

N
|
N

T
x
W

Y

I

G

G

P

P

R

R

M

M

-

D

-

F

-

I

-

P

-

G

-

I

-

D

-

G

-

P

L

S

E

E

N

G

D

V

G

P

R
|
R

V
|
V

V

L

S

A

N
x
C

F

F

I

S

V

N

Q

N

A

L

L

L

Q

R

G

R

Q

E

P

P

L

L

T

K

V

L

Y
x
V

G

D

R

G

G

G

E

E

Q

S

T

Q

R
|
R

S

T

F

F

C

V

Y

Y

V

I

S

N

D

D

L

A

V

I

D

E

G

A

L

V

I

L

R

L

L

M

M

I

N

E

G

N

D

P

H

E

L

R

G

A

P

N

-

G

-

H

-

I

V

F

N

N

L

V

G

G

N

N

P

P

-

N

S

N

E

E

Y

V

T

T

I
x
V

L

R

Q

Q

L

L

A

A

E

E

L

M

I

M

R

T

D

E

R

V

I

Y

-

A

-

K

-

V

-

S

-

G

-

E

-

G

-

A

-

I

-

E

D

S

P

P

A

T

L

V

P

D

I

V

-

S

-

K

E

E

F
|
F

R
x
Y

P

G

L

E

P

G

Q
x
Y

D

D

D
|
D

P

S

Q

D

Q

K

R
|
R

R

I

P

P

D

D

I

M

S

T

R

I

A

I

Q

N

A

R

W

Q

L

L

K

G

W

W

Q

N

P

P

L

K

V

T

S

S

V

L

Q

W

D

D

G

L

L

L

D

E

R

S

T

T

I

L

A

T

D

-

F

Y

R

Q

D

H

R

R

Q

T

Q

Y

A

A

A

E

A

A

T

V

binding nicotinamide-adenine-dinucleotide: G17 (= G8), G20 (= G11), F21 (= F12), I22 (= I13), D42 (= D32), V43 (≠ N33), I68 (≠ H56), N69 (≠ D57), I70 (= I58), L89 (= L72), A90 (= A73), A91 (≠ C74), C93 (≠ A76), S131 (= S114), T132 (= T115), Y178 (= Y144), K182 (= K148), P205 (≠ I171), N207 (= N173), W208 (≠ T174), R228 (= R185)
binding uridine-5'-diphosphate: P95 (= P78), R175 (vs. gap), R228 (= R185), V229 (= V186), C232 (≠ N189), V246 (≠ Y203), R253 (= R210), V291 (≠ I244), F323 (= F263), Y324 (≠ R264), Y328 (≠ Q268), D330 (= D270), R334 (= R274)

6dntA Udp-n-acetylglucosamine 4-epimerase from methanobrevibacter ruminantium m1 in complex with udp-n-acetylmuramic acid (see paper)
30% identity, 86% coverage: 3:280/325 of query aligns to 5:282/310 of 6dntA

query
sites
6dntA

R

N

I

V

L

V

V

V

T

T

G
|
G

G

G

A

L

G
|
G

F
|
F

I
|
I

G

G

S

S

H

H

L

I

I

V

D

D

R

A

L

L

M

I

S

D

A

-

G

D

H

N

E

K

V

V

I

T

C

I

L

I

D
|
D

N
|
N

Y
x
L

F
x
S

T
x
S

G
|
G

R

K

K

M

H

E

N

N

V

L

A

N

Q

N

W

P

Y

-

G

N

H

H

P

E

R

N

F

L

E

T

L

I

I

I

R

K

H

E

D
|
D

I
x
L

T

M

D

D

P

A

-

D

-

L

-

E

-

K

I

I

R

L

L

K

E

D

V

K

D

D

Q

Y

I

V

Y

F

H

H

L
|
L

A
|
A

C
x
A

P

L

A
|
A

S

S

-

V

-

P

-

G

-

S

-

V

-

A

-

E

P

P

V

L

H

R

Y

Y

Q

N

Y

Q

N

N

P

N

I

I

K

D

T

A

A

S

K

L

T

K

S

L

F

F

L

I

G

A

T

C

V

K

N

N

M

-

L

-

G

-

L

-

A

-

K

N

R

N

V

I

K

K

A

-

R

K

L

V

L

I

M

F

A
x
S

S
|
S

T
x
S

S
|
S

E
x
A

V

V

Y

Y

G

G

D

E

P

N

H

P

V

N

H

M

P

P

Q

L

T

K

E

E

D

S

Y

-

W

-

G

E

N

N

V

F

N

L

P

P

I

C

G

-

I

-

R

-

S

S

C

P

Y
|
Y

D

A

E

A

G

Q

K
|
K

R

A

V

S

A

C

E

E

T

L

L

Y

C

L

F

K

D

S

Y

F

H

H

R

E

Q

S

H

Y

N

G

L

L

E

D

I

Y

R

V

V

A

A

L

R

R

I
x
Y

F
|
F

N
|
N

T
x
V

Y

F

G

G

P

P

R

R

M

Q

L

D

E

E

N

N

D

S

-

P

-

Y

G

A

R
x
A

V
|
V

V

I

S

P

N
x
K

F

F

I

I

V

S

Q

A

A

I

L

L

Q

N

G

G

Q

E

P

S

L

P

T
x
V

V
x
I

Y
|
Y

G

G

R

D

G

G

E
|
E

Q
|
Q

T

S

R
|
R

S

D

F

F

C

I

Y

Y

V

V

S

K

D

E

L

I

V

A

D

K

G

A

L

N

I

I

R

L

L

S

M

A

N

E

G

S

D

D

H

Y

L

N

G

G

P

V

V

I

N

N

L

V

G

A

N

L

P

G

S

K

E

S

Y

M

T

T

I
|
I

L

N

Q

R

L

L

A

F

E

E

L

I

I

I

R

S

D

D

R

V

I

L

D

E

P

S

A

D

L

I

P

D

I

V

E

K

F

Y

R

L

P

D

L

E

P
x
R

Q

P

D

G

D
|
D

P

I

Q

K

Q
x
H

R

S

R

L

P

A

D

D

I

I

S

S

R

N

active site: S120 (≠ T115), S121 (= S116), A122 (≠ E117), Y144 (= Y144), K148 (= K148), A187 (≠ R185)
binding (2R)-2-{[(2R,3R,4R,5S,6R)-3-(acetylamino)-2-{[(S)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-5-hydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-4-yl]oxy}propanoic acid: A80 (= A76), S120 (≠ T115), S121 (= S116), Y144 (= Y144), F172 (= F172), N173 (= N173), A187 (≠ R185), V188 (= V186), K191 (≠ N189), V203 (≠ T201), I204 (≠ V202), Y205 (= Y203), Q210 (= Q208), R212 (= R210), I246 (= I244), R269 (≠ P267), D272 (= D270)
binding nicotinamide-adenine-dinucleotide: G10 (= G8), G13 (= G11), F14 (= F12), I15 (= I13), D33 (= D32), N34 (= N33), L35 (≠ Y34), S36 (≠ F35), S37 (≠ T36), G38 (= G37), D57 (= D57), L58 (≠ I58), L76 (= L72), A77 (= A73), A78 (≠ C74), A80 (= A76), S118 (≠ A113), S119 (= S114), Y144 (= Y144), K148 (= K148), Y171 (≠ I171), V174 (≠ T174)
binding zinc ion: E209 (= E207), H275 (≠ Q273)

3eheA Crystal structure of udp-glucose 4 epimerase (gale-1) from archaeoglobus fulgidus
28% identity, 93% coverage: 4:304/325 of query aligns to 2:284/290 of 3eheA

query
sites
3eheA

I

I

L

V

V

V

T

T

G
|
G

G

G

A

A

G
|
G

F
|
F

I
|
I

G

G

S

S

H

H

L

V

I

V

D

D

R

K

L

L

M

-

S

S

A

E

G

S

H

N

E

E

V

I

I

V

C

V

L

I

D
|
D

N
|
N

Y
x
L

F
x
S

T
x
S

G
|
G

R

N

K

E

H

E

N

F

V

V

A

N

Q

E

W

A

Y

A

G

R

H

L

P

V

R

K

F

A

E
x
D

L
|
L

I

A

R

A

H

D

D

D

I

I

T

K

D

D

P

Y

I

L

R

K

L

-

E

G

V

A

D

E

Q

E

I

V

Y

W

H

H

L
x
I

A
|
A

C

-

P

-

A
|
A

S

N

P

P

-

D

V

V

H

R

Y

G

Q

A

Y

E

N

N

P

P

I

D

K

E

T

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active site: T111 (= T115), S112 (= S116), T113 (≠ E117), Y135 (= Y144), K139 (= K148)
binding nicotinamide-adenine-dinucleotide: G6 (= G8), G9 (= G11), F10 (= F12), I11 (= I13), D29 (= D32), N30 (= N33), L31 (≠ Y34), S32 (≠ F35), S33 (≠ T36), G34 (= G37), D49 (≠ E52), L50 (= L53), I68 (≠ L72), A69 (= A73), A70 (= A76), T109 (≠ A113), Y135 (= Y144), K139 (= K148), F162 (≠ I171), A163 (≠ F172), N164 (= N173), V165 (≠ T174)

8gjhA Salmonella arna (see paper)
27% identity, 93% coverage: 2:304/325 of query aligns to 299:632/639 of 8gjhA

query
sites
8gjhA

M

I

R

R

I

V

L

L

V

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T

L

G

G

G

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A

N

G

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F

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Y

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D

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binding uridine-5'-diphosphate-glucuronic acid: A376 (= A76), P378 (= P78), Y381 (= Y81), S416 (= S116), R443 (= R141), N475 (= N173), R493 (= R185), A494 (≠ V186), K509 (≠ T201), I511 (≠ Y203), R518 (= R210), I557 (= I244), Y592 (vs. gap), R602 (= R274)

Query Sequence

>Synpcc7942_1149 FitnessBrowser__SynE:Synpcc7942_1149
MMRILVTGGAGFIGSHLIDRLMSAGHEVICLDNYFTGRKHNVAQWYGHPRFELIRHDITD
PIRLEVDQIYHLACPASPVHYQYNPIKTAKTSFLGTVNMLGLAKRVKARLLMASTSEVYG
DPHVHPQTEDYWGNVNPIGIRSCYDEGKRVAETLCFDYHRQHNLEIRVARIFNTYGPRML
ENDGRVVSNFIVQALQGQPLTVYGRGEQTRSFCYVSDLVDGLIRLMNGDHLGPVNLGNPS
EYTILQLAELIRDRIDPALPIEFRPLPQDDPQQRRPDISRAQAWLKWQPLVSVQDGLDRT
IADFRDRQQAAATLIADRLPEGSIS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory