SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Synpcc7942_1198 FitnessBrowser__SynE:Synpcc7942_1198 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6awaA 1.83 angstrom resolution crystal structure of dihydrolipoyl dehydrogenase from pseudomonas putida in complex with fad and adenosine-5'-monophosphate.
38% identity, 97% coverage: 7:470/479 of query aligns to 5:461/475 of 6awaA

query
sites
6awaA

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active site: L45 (= L40), C49 (= C44), C54 (= C49), S57 (= S52), V191 (≠ Y188), E195 (= E192), F449 (≠ H458), H451 (= H460), E456 (= E465)
binding adenosine monophosphate: I187 (= I184), E211 (= E208), A212 (= A209), L213 (= L210), V245 (= V241), V277 (≠ T277)
binding flavin-adenine dinucleotide: I10 (= I12), G13 (= G15), P14 (≠ Y16), G15 (= G17), E34 (= E36), K35 (≠ A37), T48 (= T43), C49 (= C44), G53 (= G48), C54 (= C49), K58 (= K53), H121 (≠ R118), G122 (= G119), S151 (≠ T148), G152 (= G149), I192 (= I189), R279 (= R279), G318 (= G323), D319 (= D324), M325 (= M330), L326 (= L331), A327 (= A332), Y358 (≠ F363)

Sites not aligning to the query:

active site: 474, 475

2yquB Crystal structures and evolutionary relationship of two different lipoamide dehydrogenase(e3s) from thermus thermophilus
37% identity, 97% coverage: 7:470/479 of query aligns to 2:444/455 of 2yquB

query
sites
2yquB

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active site: P11 (≠ Y16), L36 (= L40), C40 (= C44), C45 (= C49), S48 (= S52), G72 (= G80), V73 (= V81), V177 (≠ Y188), E181 (= E192), S314 (≠ A336), H432 (= H458), H434 (= H460), E439 (= E465)
binding carbonate ion: A310 (= A332), S314 (≠ A336), S423 (= S449), D426 (≠ E452)
binding flavin-adenine dinucleotide: G8 (= G13), G10 (= G15), P11 (≠ Y16), G12 (= G17), E31 (= E36), K32 (≠ A37), G38 (= G42), T39 (= T43), C40 (= C44), R42 (≠ N46), G44 (= G48), C45 (= C49), K49 (= K53), T110 (≠ R118), A111 (≠ G119), T137 (= T148), G138 (= G149), I178 (= I189), Y265 (≠ S282), G301 (= G323), D302 (= D324), M308 (= M330), L309 (= L331), A310 (= A332), H311 (= H333)

2yquA Crystal structures and evolutionary relationship of two different lipoamide dehydrogenase(e3s) from thermus thermophilus
37% identity, 97% coverage: 7:470/479 of query aligns to 2:444/455 of 2yquA

query
sites
2yquA

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T

V

P

P

-

E

-

A

-

K

G

G

S

A

P

R

V

V

V

E

I

L

E

E

L

G

A

G

D

-

F

-

E

-

T

-

K

-

E

-

L

-

V

-

E

E

V

V

L

L

E

E

V

A

D

D

A

R

C

V

L

L

V

V

A

A

T

V

G

G

R

R

I

R

P

P

S
x
Y

T

T

K

E

N

G

L

L

G

S

L

L

E

E

T

N

V

A

A

G

V

L

E

S

V

T

D

D

R

E

R

R

G

G

F

R

I

I

P

P

V

V

D

D

D

E

G

H

M

L

R

R

V

T

L

R

R

-

D

-

S

-

K

-

P

-

V

V

P

P

H

H

L

I

F

Y

A

A

V

I

G
|
G

D
|
D

A

V

T

V

G

R

K

G

L

P

M
|
M

L
|
L

A
|
A

H

H

A

K

A

A

A
x
S

A

E

Q

E

G

G

V

I

V

A

A

A

I

V

E

E

N

H

I

M

T

V

G

R

H

G

P

F

R

G

S

H

V

V

D

D

Y

Y

R

Q

S

A

I

I

P

P

A

S

A

V

T

V

F

Y

T

T

H

H

P

P

E

E

I

I

S

A

V

V

G

G

L

Y

S

T

E

E

A

E

D

E

A

L

K

K

A

A

L

Q

G

G

G

I

Q

P

E

-

G

-

F

Y

E

K

V

V

G

G

S

-

V

-

R

K

S

F

Y

P

F

Y

K

S

A

A

N

S

S

G

K

R

A

A

L

R

A

A

E

M

A

G

D

E

A

T

D

E

G

G

F

F

A

I

K

K

V

V

L

L

F

A

R

H

K

A

D

K

T

T

G

D

E

R

V

I

L

L

G

G

A

V

H

H

I

G

F

I

G

G

L

A

H

R

A

V

A

G

D

D

L

V

I

L

Q

A

E

E

V

A

A

A

N

L

A

A

I

L

A

F

R

F

R

K

Q

A

S

S

V

A

K

E

E

D

L

L

A

G

F

R

E

A

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H
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T

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H
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H

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T

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L

L

S

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E
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E

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L

E

K

S

E

A

A

active site: P11 (≠ Y16), L36 (= L40), C40 (= C44), C45 (= C49), S48 (= S52), G72 (= G80), V73 (= V81), V177 (≠ Y188), E181 (= E192), S314 (≠ A336), H432 (= H458), H434 (= H460), E439 (= E465)
binding flavin-adenine dinucleotide: G8 (= G13), G10 (= G15), P11 (≠ Y16), G12 (= G17), E31 (= E36), K32 (≠ A37), G38 (= G42), T39 (= T43), C40 (= C44), R42 (≠ N46), G44 (= G48), C45 (= C49), K49 (= K53), T110 (≠ R118), A111 (≠ G119), T137 (= T148), G138 (= G149), S157 (= S168), I178 (= I189), Y265 (≠ S282), G301 (= G323), D302 (= D324), M308 (= M330), L309 (= L331), A310 (= A332)

2eq7A Crystal structure of lipoamide dehydrogenase from thermus thermophilus hb8 with psbdo
37% identity, 97% coverage: 7:470/479 of query aligns to 2:444/452 of 2eq7A

query
sites
2eq7A

Y

Y

D

D

V

L

I

L

V

V

I

I

G

G

A

A

G
|
G

Y
x
P

G
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G

G

G

F

Y

D

V

A

A

K

I

H

R

A

A

C

A

E

Q

K

L

G

G

L

M

K

K

T

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A

G

I

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V

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E
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E

A
x
K

-

E

R

K

D

A

L
|
L

G

G

G
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G

T
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T

C
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C

V

L

N
x
R

R

V

G
|
G

C
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C

V

I

P

P

S
|
S

K
|
K

A

A

L

L

A

E

A

T

S

T

G

E

R

R

V

I

R

Y

E

E

I

A

T

K

D

K

T

G

D

-

H

-

L

-

Q

-

N

L

F

L

G

G

I

A

Q

K

V

V

Q

K

G
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G

V
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V

S

E

F

L

D

D

R

L

Q

P

A

A

I

L

A

M

D

A

H

H

A

K

A

D

N

K

L

V

V

V

D

Q

T

A

V

N

R

T

T

Q

N

G

L

V

G

E

N

F

T

L

L

F

K

K

R

K

L

N

G

G

A

I

E

A

I

R

L

H

M

Q

G

G

R
x
T

G
x
A

R

R

L

F

A

L

G

S

S

E

Q

R

R

K

V

V

T

L

V

V

T

-

A

-

D

E

G

E

T

T

E

G

K

E

T

E

Y

L

S

E

A

A

R

R

D

Y

V

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I

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L

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A

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T
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T

G
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A

P

P

F

L

V

I

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P

G
x
W

I

A

E

Q

I

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D

G

Y

K

E

T

R

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V

F

V

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T

S
|
S

D

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D

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A

A

L

L

K

S

L

F

E

P

T

E

L

V

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P

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K

W

R

I

L

A

I

I

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G
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G

S

G

G
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G

Y
x
V

I
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I

G

G

L

L

E
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E

F

L

S

G

D

V

V

V

Y

W

T

H

A

R

L

L

G

G

C

A

E

E

V

V

T

I

M

V

I

L

E
|
E

A
x
Y

L

M

D

D

R

R

L

I

L

L

P

P

T

T

F

M

D

D

P

L

D

E

V

V

A

S

K

R

V

A

A

A

Q

E

R

R

S

-

L

V

L

F

D

K

G

K

R

Q

D

G

I

L

E

T

T

I

R

R

T

T

G

G

V

V

L

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A
x
V

R

T

K

A

I

V

T

V

P

P

-

E

-

A

-

K

G

G

S

A

P

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V

E

I

L

E

E

L

G

A

G

D

-

F

-

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-

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-

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-

L

-

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-

E

E

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L

E

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V

A

D

D

A

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C

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L

L

V

V

A

A

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x
V

G
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G

R
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R

I

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P

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S
x
Y

T

T

K

E

N

G

L

L

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L

L

E

E

T

N

V

A

A

G

V

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E

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R

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G

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R

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P

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D

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H

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R

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L

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R

-

D

-

S

-

K

-

P

-

V

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P

H

H

L

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Y

A

A

V

I

G

G

D
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D

A

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V

G

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G

L

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M
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M

L
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L

A
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A

H
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H

A

K

A

A

A
x
S

A

E

Q

E

G

G

V

I

V

A

A

A

I

V

E

E

N

H

I

M

T

V

G

R

H

G

P

F

R

G

S

H

V

V

D

D

Y

Y

R

Q

S

A

I

I

P

P

A

S

A
x
V

T

V

F
x
Y

T

T

H

H

P

P

E

E

I

I

S

A

V

V

G

G

L

Y

S

T

E

E

A

E

D

E

A

L

K

K

A

A

L

Q

G

G

G

I

Q

P

E

-

G

-

F

Y

E

K

V

V

G

G

S

-

V

-

R

K

S

F

Y

P

F

Y

K

S

A

A

N

S

S

G

K

R

A

A

L

R

A

A

E

M

A

G

D

E

A

T

D

E

G

G

F

F

A

I

K

K

V

V

L

L

F

A

R

H

K

A

D

K

T

T

G

D

E

R

V

I

L

L

G

G

A

V

H

H

I

G

F

I

G

G

L

A

H

R

A

V

A

G

D

D

L

V

I

L

Q

A

E

E

V

A

A

A

N

L

A

A

I

L

A

F

R

F

R

K

Q

A

S

S

V

A

K

E

E

D

L

L

A

G

F

R

E

A

V

P

H
|
H

T

A

H
|
H

P

P

T

S

L

L

S

S

E
|
E

V

I

I

L

E

K

S

E

A

A

active site: P11 (≠ Y16), L36 (= L40), C40 (= C44), C45 (= C49), S48 (= S52), G72 (= G80), V73 (= V81), V177 (≠ Y188), E181 (= E192), S314 (≠ A336), H432 (= H458), H434 (= H460), E439 (= E465)
binding flavin-adenine dinucleotide: G10 (= G15), P11 (≠ Y16), G12 (= G17), E31 (= E36), K32 (≠ A37), G38 (= G42), T39 (= T43), C40 (= C44), R42 (≠ N46), G44 (= G48), C45 (= C49), K49 (= K53), T110 (≠ R118), A111 (≠ G119), T137 (= T148), G138 (= G149), S157 (= S168), I178 (= I189), R262 (= R279), Y265 (≠ S282), D302 (= D324), M308 (= M330), L309 (= L331), A310 (= A332), H311 (= H333), Y341 (≠ F363)
binding nicotinamide-adenine-dinucleotide: W146 (≠ G157), G174 (= G185), G176 (= G187), V177 (≠ Y188), I178 (= I189), E197 (= E208), Y198 (≠ A209), V231 (≠ A243), V260 (≠ T277), G261 (= G278), R262 (= R279), M308 (= M330), L309 (= L331), V339 (≠ A361)

P11959 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; EC 1.8.1.4 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
36% identity, 96% coverage: 5:466/479 of query aligns to 8:452/470 of P11959

query
sites
P11959

F

I

D

E

Y

T

D

E

V

T

I

L

V

V

I

V

G

G

A

A

G

G

Y

P

G

G

F

Y

D

V

A

A

K

I

H

R

A

A

C

A

E

Q

K

L

G

G

L

Q

K

K

T

V

A

T

I

I

V

V

E
|
E

A
x
K

R
x
G

D
x
N

L
|
L

G
|
G

T
x
V

C
|
C

V

L

N

N

R

V

G

G

C

C

V

I

P

P

S

S

K
|
K

A

A

L

L

L

I

A

S

A

A

S

S

G

H

R

R

V

Y

R

E

E

Q

I

-

T

-

D

-

T

A

D

K

H

H

L

S

Q

E

N

E

F

M

G

G

I

I

Q

K

V

A

Q

E

G

N

V

V

S

T

F

I

D

D

R

F

Q

A

A

K

I

V

A

Q

D

E

H

W

A

K

A

A

N

S

L

V

V

V

D

K

T

K

V

L

R

T

T

G

N

G

L

V

G

E

N

G

T

L

L

L

K

K

R

G

L

N

G

K

A

V

E

E

I

I

L

V

M

K

G

G

R

E

G

A

R

Y

L

F

A

V

G

D

S

A

Q

N

R

T

V

V

T

R

V

V

T

V

A

N

A

G

D

D

G

-

T

S

E

A

K

Q

T

T

Y

Y

S

T

A

F

R

K

D

N

V

A

I

I

L

I

A

A

T

T

G

G

S

S

D

R

P

P

F

I

V

E

P

L

P

P

G

N

I

F

E

K

I

F

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K

R

T

-

V

I

F

L

T

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S

S

D

T

D

G

A

A

L

L

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N

L

L

E

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E

L

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W

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I

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A

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I

V

I

I

G

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G

G

G

Y

Y

I

I

G

G

L

I

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E

F

L

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G

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T

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A

Y

Y

T

A

A

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L

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G

G

C

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T

M

I

I

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E

E

A

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A

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E

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L

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G

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F

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D

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A

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K

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A

I

Q

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S

R

L

L

L

-

D

K

G

K

R

K

D

G

I

V

E

E

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V

R

V

T

T

G

N

V

A

L

L

A

A

R

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-

G

-

A

-

E

K

E

I

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T

E

P

D

G

G

S

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T

V

V

V

T

I

Y

E

E

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-

A

A

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N

F

G

E

E

T

T

K

K

E

-

L

-

V

-

E

-

V

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A

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D

A

Y

C

V

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L

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V

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N

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L

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K

-

P

S

V

V

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P

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N

L

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F

A

A

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G

D
|
D

A

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G

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M

A

L

L

A
|
A

H

H

A

K

A

A

A

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A

Y

Q

E

G

G

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K

V

V

A

A

I

A

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E

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A

I

I

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A

G

G

H

H

P

P

R

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S

A

V

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D

D

Y

Y

R

V

S

A

I

I

P

P

A

A

A

V

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V

F

F

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S

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D

P

P

E

E

I

C

S

A

S

S

V

V

G

G

L

Y

S

F

E

E

A

Q

D

Q

A

A

K

K

A

-

L

-

G

-

Q

D

E

E

G

G

F

I

E

D

V

V

G

I

S

A

V

A

R

K

S

F

Y

P

F

F

K

A

A

A

N

N

S

G

K

R

A

A

L

L

A

A

E

L

A

N

D

D

A

T

D

D

G

G

F

F

A

L

K

K

V

L

L

V

F

V

R

R

K

K

D

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T

D

G

G

E

V

V

I

L

I

G

G

A

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I

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F

I

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G

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P

H

N

A

A

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D

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M

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A

E

E

V

L

A

G

N

L

A

A

I

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A

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R

A

R

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M

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A

K

E

E

D

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I

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A

F

L

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I

H

H

T

A

H

H

P

P

T

T

L

L

S

G

E

E

V

I

39:47 (vs. 36:44, 56% identical) binding
K56 (= K53) binding
D314 (= D324) binding
A322 (= A332) binding

1ebdA Dihydrolipoamide dehydrogenase complexed with the binding domain of the dihydrolipoamide acetylase (see paper)
36% identity, 96% coverage: 5:466/479 of query aligns to 2:446/455 of 1ebdA

query
sites
1ebdA

F

I

D

E

Y

T

D

E

V

T

I

L

V

V

I

V

G
|
G

A

A

G
|
G

Y
x
P

G

G

F

Y

D

V

A

A

K

I

H

R

A

A

C

A

E

Q

K

L

G

G

L

Q

K

K

T

V

A

T

I

I

V
|
V

E
|
E

A
x
K

R

G

D

N

L
|
L

G

G

G
|
G

T
x
V

C
|
C

V

L

N

N

R

V

G
|
G

C
|
C

V

I

P

P

S
|
S

K
|
K

A

A

L

L

L

I

A

S

A

A

S

S

G

H

R

R

V

Y

R

E

E

Q

I

-

T

-

D

-

T

A

D

K

H

H

L

S

Q

E

N

E

F

M

G

G

I

I

Q

K

V

A

Q

E

G
x
N

V
|
V

S

T

F

I

D

D

R

F

Q

A

A

K

I

V

A

Q

D

E

H

W

A

K

A

A

N

S

L

V

V

V

D

K

T

K

V

L

R

T

T

G

N

G

L

V

G

E

N

G

T

L

L

L

K

K

R

G

L

N

G

K

A

V

E

E

I

I

L

V

M

K

G

G

R
x
E

G
x
A

R

Y

L

F

A

V

G

D

S

A

Q

N

R

T

V

V

T

R

V

V

T

V

A

N

A

G

D

D

G

-

T

S

E

A

K

Q

T

T

Y

Y

S

T

A

F

R

K

D

N

V

A

I

I

L

I

A

A

T
|
T

G
|
G

S

S

D

R

P

P

F

I

V

E

P

L

P

P

G

N

I

F

E

K

I

F

D

S

G

N

K

R

T

-

V

I

F

L

T

D

S

S

D

T

D

G

A

A

L

L

K

N

L

L

E

G

T

E

L

V

P

P

Q

K

W

S

I

L

A

V

I

V

I

I

G

G

S

G

G

G

Y
|
Y

I
|
I

G

G

L

I

E
|
E

F

L

S

G

D

T

V

A

Y

Y

T

A

A

N

L

F

G

G

C

T

E

K

V

V

T

T

M

I

I

L

E

E

A

G

L

A

D

G

R

E

L

I

L

L

P

S

T

G

F

F

D

E

P

K

D

Q

V

M

A

A

K

A

V

I

A

I

Q

K

R

K

S

R

L

L

L

-

D

K

G

K

R

K

D

G

I

V

E

E

T

V

R

V

T

T

G

N

V

A

L

L

A

A

R

K

-

G

-

A

-

E

K

E

I

R

T

E

P

D

G

G

S

V

P

T

V

V

V

T

I

Y

E

E

L

-

A

A

D

N

F

G

E

E

T

T

K

K

E

-

L

-

V

-

E

-

V

T

L

I

E

D

V

A

D

D

A

Y

C

V

L

L

V

V

A

T

T

V

G

G

R
|
R

I

R

P

P

S

N

T

T

K

D

N

E

L

L

G

G

L

L

E

E

T

Q

V

I

A

G

V

I

E

K

V

M

D

T

R

N

R

R

G

G

F

L

I

I

P

E

V

V

D

D

D

Q

G

Q

M

C

R

R

V

T

L

-

R

-

D

-

S

-

K

-

P

S

V

V

P

P

H

N

L

I

F

F

A

A

V

I

G

G

D
|
D

A

I

T

V

G

P

K

G

L

P

M
x
A

L
|
L

A
|
A

H

H

A

K

A

A

A
x
S

A

Y

Q

E

G

G

V

K

V

V

A

A

I

A

E

E

N

A

I

I

T

A

G

G

H

H

P

P

R

S

S

A

V

V

D

D

Y

Y

R

V

S

A

I

I

P

P

A

A

A

V

T

V

F

F

T

S

H

D

P

P

E

E

I

C

S

A

S

S

V

V

G

G

L

Y

S

F

E

E

A

Q

D

Q

A

A

K

K

A

-

L

-

G

-

Q

D

E

E

G

G

F

I

E

D

V

V

G

I

S

A

V

A

R

K

S

F

Y

P

F

F

K

A

A

A

N

N

S

G

K

R

A

A

L

L

A

A

E

L

A

N

D

D

A

T

D

D

G

G

F

F

A

L

K

K

V

L

L

V

F

V

R

R

K

K

D

E

T

D

G

G

E

V

V

I

L

I

G

G

A

A

H

Q

I

I

F

I

G

G

L

P

H

N

A

A

A

S

D

D

L

M

I

I

Q

A

E

E

V

L

A

G

N

L

A

A

I

I

A

E

R

A

R

G

Q

M

S

T

V

A

K

E

E

D

L

I

A

A

F

L

E

T

V

I

H
|
H

T

A

H
|
H

P

P

T

T

L

L

S

G

E
|
E

V

I

active site: P13 (≠ Y16), L37 (= L40), C41 (= C44), C46 (= C49), S49 (= S52), N74 (≠ G80), V75 (= V81), Y180 (= Y188), E184 (= E192), S320 (≠ A336), H438 (= H458), H440 (= H460), E445 (= E465)
binding flavin-adenine dinucleotide: G10 (= G13), G12 (= G15), P13 (≠ Y16), V32 (= V35), E33 (= E36), K34 (≠ A37), G39 (= G42), V40 (≠ T43), C41 (= C44), G45 (= G48), C46 (= C49), K50 (= K53), E112 (≠ R118), A113 (≠ G119), T141 (= T148), G142 (= G149), Y180 (= Y188), I181 (= I189), R268 (= R279), D308 (= D324), A314 (≠ M330), L315 (= L331), A316 (= A332)

P14218 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex; EC 1.8.1.4 from Pseudomonas fluorescens (see 2 papers)
38% identity, 97% coverage: 7:470/479 of query aligns to 5:461/478 of P14218

query
sites
P14218

Y

F

D

D

V

V

I

V

V

V

I

I

G

G

A

A

G

G

Y

P

G

G

F

Y

D

V

A

A

K

I

H

R

A

A

C

A

E

Q

K

L

G

G

L

L

K

K

T

T

A

A

I

C

V

I

E
|
E

A
x
K

R
x
Y

-
x
I

-
x
G

-
x
K

-
x
E

-
x
G

-
x
K

-
x
V

D
x
A

L
|
L

G
|
G

T
|
T

C
|
C

V

L

N

N

R

V

G

G

C
|
C

V

I

P

P

S

S

K
|
K

A

A

L

L

A

D

A

S

S

S

G

Y

R

K

V

Y

R

H

E

E

I

A

T

K

D

E

T

A

D

-

H

-

L

F

Q

K

N

V

F

H

G

G

I

I

Q

E

V

A

Q

K

G

G

V

V

S

T

F

I

D

D

R

V

Q

P

A

A

I

M

A

V

D

A

H

R

A

K

A

A

N

N

L

I

V

V

D

K

T

N

V

L

R

T

T

G

N

G

L

I

G

A

N

T

T

L

L

F

K

K

R

A

L

N

G

G

A

V

E

T

I

S

L

F

M

E

G

G

R

H

G
|
G

R

K

L

L

A

L

G

A

S

N

Q

K

R

Q

V

V

T

E

V

V

T

T

A

G

A

L

D

D

G

G

T

K

E

T

K

Q

T

V

Y

L

S

E

A

A

R

E

D

N

V

V

I

I

L

I

A

A

T

S

G

G

S

S

D

R

P

P

F

V

V

E

P

I

P

P

G

P

I

A

E

P

I

L

D

S

G

D

K

D

T

I

V

I

F

V

T

D

S

S

D

T

D

G

A

A

L

L

K

E

L

F

E

Q

T

A

L

V

P

P

Q

K

W

K

I

L

A

G

I

V

I

I

G

G

S

A

G

G

Y

V

I

I

G

G

L

L

E

E

F

L

S

G

D

S

V

V

Y

W

T

A

A

R

L

L

G

G

C

A

E

E

V

V

T

T

M

V

I

L

E

E

A

A

L

L

D

D

R

K

L

F

L

L

P

P

T

A

F

A

D

D

P

E

D

Q

V

I

A

A

K

K

V

E

A

A

Q

L

R

K

S

V

L

L

L

T

-

K

D

Q

G

G

R

L

D

N

I

I

E

R

T

L

R

G

T

A

G

R

V

V

L

T

A

A

R

S

K

E

I

V

T

K

P

K

G

K

S

Q

-

V

P

T

V

V

V

T

I

F

E

T

L

D

A

A

D

N

F

G

E

E

T

Q

K

K

E

E

L

-

V

-

E

-

V

-

L

-

E

T

V

F

D

D

A

K

C

L

L

I

V

V

A

A

T

V

G

G

R

R

I

R

P

P

S

V

T

T

K

T

N

D

L

L

G

L

L

A

E

A

T

D

V

S

A

G

V

V

E

T

V

L

D

D

R

E

R

R

G

G

F

F

I

I

P

Y

V

V

D

D

G

H

M

C

R

K

V

T

L

-

R

-

D

-

S

-

K

-

P

S

V

V

P

P

H

G

L

V

F

F

A

A

V

I

G

G

D
|
D

A

V

T

V

G

R

K

G

L

A

M

M

L

L

A
|
A

H

H

A

K

A

A

A

S

A

E

Q

E

G

G

V

V

V

M

A

V

I

A

E

E

N

R

I

I

T

A

G

G

H

H

P

K

R

A

S

Q

V

M

D

N

Y

Y

R

D

S

L

I

I

P

P

A

S

A

V

T

I

F

Y

T

T

H

H

P

P

E

E

I

I

S

A

S

W

V

V

G

G

L

K

S

T

E

E

A

Q

D

T

A

L

K

K

A

A

L

-

G

-

Q

-

E

E

G

G

F

V

E

E

V

V

G

N

S

V

V

G

R

T

S

F

Y

P

F

F

K

A

A

A

N

S

S

G

K

R

A

A

L

M

A

A

E

A

A

N

D

D

A

T

D

T

G

G

F

L

A

V

K

K

V

V

L

I

F

A

R

D

K

A

D

K

T

T

G

D

E

R

V

V

L

L

G

G

A

V

H

H

I

V

F

I

G

G

L

P

H

S

A

A

D

E

L

L

I

V

Q

Q

E

Q

V

G

A

A

N

I

A

G

I

M

A

E

R

F

R

G

Q

T

S

S

V

A

K

E

E

D

L

L

A

G

F

M

E

M

V

V

H

F

T

S

H

H

P

P

T

T

L

L

S

S

E

E

V

A

I

L

E

H

S

E

A

A

34:49 (vs. 36:44, 38% identical) binding
C49 (= C44) modified: Disulfide link with 54, Redox-active
C54 (= C49) modified: Disulfide link with 49, Redox-active
K58 (= K53) binding
G122 (= G119) binding
D319 (= D324) binding
A327 (= A332) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

5u8wA Dihydrolipoamide dehydrogenase (lpdg) from pseudomonas aeruginosa bound to nadh (see paper)
38% identity, 97% coverage: 7:470/479 of query aligns to 4:460/473 of 5u8wA

query
sites
5u8wA

Y

F

D

D

V

V

I

V

V

V

I
|
I

G

G

A

A

G
|
G

Y
x
P

G
|
G

G

G

F

Y

D

V

A

A

K

I

H

R

A

A

C

A

E

Q

K

L

G

G

L

L

K

K

T

T

A

A

I

C

V

I

E
|
E

A
x
K

R

Y

-

I

-

G

-

K

-

E

-

G

-

K

-

V

D

A

L
|
L

G

G

G
|
G

T
|
T

C
|
C

V

L

N

N

R

V

G
|
G

C
|
C

V

I

P

P

S
|
S

K
|
K

A

A

L

L

A

D

A

S

S

S

G

Y

R

K

V

Y

R

H

E

E

I

A

T

K

D

E

T

A

D

-

H

-

L

F

Q

K

N

V

F

H

G

G

I

I

Q

E

V

A

Q

K

G
|
G

V
|
V

S

T

F

I

D

D

R

V

Q

P

A

A

I

M

A

V

D

A

H

R

A

K

A

A

N

N

L

I

V

V

D

K

T

N

V

L

R

T

T

G

N

G

L

I

G

A

N

T

T

L

L

F

K

K

R

A

L

N

G

G

A

V

E

T

I

S

L

F

M

E

G

G

R
x
H

G
|
G

R

K

L

L

A

L

G

A

S

N

Q

K

R

Q

V

V

T

E

V

V

T

T

A

G

A

L

D

D

G

G

T

K

E

T

K

Q

T

V

Y

L

S

E

A

A

R

E

D

N

V

V

I

I

L

I

A
|
A

T
x
S

G
|
G

S

S

D

R

P

P

F

V

V

E

P

I

P

P

G

P

I

A

E

P

I

L

D

T

G

D

K

D

T

I

V

I

F

V

T

D

S
|
S

D

T

D

G

A

A

L

L

K

E

L

F

E

Q

T

A

L

V

P

P

Q

K

W

K

I

L

A

G

I

V

I
|
I

G

G

S

A

G
|
G

Y
x
V

I
|
I

G

G

L

L

E
|
E

F

L

S

G

D

S

V

V

Y

W

T

A

A

R

L

L

G

G

C

A

E

E

V

V

T

T

M

V

I

L

E
|
E

A
|
A

L
|
L

D

D

R

K

L

F

L

L

P

P

T

A

F

A

D

D

P

E

D

Q

V

I

A

A

K

K

V

E

A

A

Q

L

R

K

S

V

L

L

L

T

-

K

D

Q

G

G

R

L

D

N

I

I

E

R

T

L

R

G

T

A

G

R

V

V

L

T

A

A

R

S

K

E

I

V

T

K

P

K

G

K

S

Q

-

V

P

T

V

V

V

T

I

F

E

T

L

D

A

A

D

N

F

G

E

E

T

Q

K

K

E

E

L

-

V

-

E

-

V

-

L

-

E

T

V

F

D

D

A

K

C

L

L

I

V

V

A
|
A

T
x
V

G
|
G

R
|
R

I

R

P

P

S

V

T

T

K

T

N

D

L

L

G

L

L

A

E

A

T

D

V

S

A

G

V

V

E

T

V

L

D

D

R

E

R

R

G

G

F

F

I

I

P

Y

V

V

D

D

G

H

M

C

R

K

V

T

L

-

R

-

D

-

S

-

K

-

P

S

V

V

P

P

H

G

L

V

F

F

A

A

V

I

G
|
G

D
|
D

A

V

T

V

G

R

K

G

L

A

M
|
M

L
|
L

A
|
A

H
|
H

A

K

A

A

A
x
S

A

E

Q

E

G

G

V

V

V

M

A

V

I

A

E

E

N

R

I

I

T

A

G

G

H

H

P

K

R

A

S

Q

V

M

D

N

Y

Y

R

D

S

L

I

I

P

P

A

S

A
x
V

T

I

F
x
Y

T

T

H

H

P

P

E

E

I

I

S

A

S

W

V

V

G

G

L

K

S

T

E

E

A

Q

D

T

A

L

K

K

A

A

L

-

G

-

Q

-

E

E

G

G

F

V

E

E

V

V

G

N

S

V

V

G

R

T

S

F

Y

P

F

F

K

A

A

A

N

S

S

G

K

R

A

A

L

M

A

A

E

A

A

N

D

D

A

T

D

T

G

G

F

L

A

V

K

K

V

V

L

I

F

A

R

D

K

A

D

K

T

T

G

D

E

R

V

V

L

L

G

G

A

V

H

H

I

V

F

I

G

G

L

P

H

S

A

A

D

E

L

L

I

V

Q

Q

E

Q

V

G

A

A

N

I

A

G

I

M

A

E

R

F

R

G

Q

T

S

S

V

A

K

E

E

D

L

L

A

G

F

M

E

M

V

V

H
x
F

T

S

H
|
H

P
|
P

T

T

L

L

S

S

E
|
E

V

A

I

L

E

H

S

E

A

A

active site: P13 (≠ Y16), L44 (= L40), C48 (= C44), C53 (= C49), S56 (= S52), G82 (= G80), V83 (= V81), V190 (≠ Y188), E194 (= E192), S330 (≠ A336), F448 (≠ H458), H450 (= H460), E455 (= E465)
binding flavin-adenine dinucleotide: I9 (= I12), G12 (= G15), P13 (≠ Y16), G14 (= G17), E33 (= E36), K34 (≠ A37), G46 (= G42), T47 (= T43), C48 (= C44), G52 (= G48), C53 (= C49), K57 (= K53), H120 (≠ R118), G121 (= G119), A149 (= A147), S150 (≠ T148), G151 (= G149), S170 (= S168), G317 (= G323), D318 (= D324), M324 (= M330), L325 (= L331), A326 (= A332), H327 (= H333), Y357 (≠ F363), H450 (= H460), P451 (= P461)
binding 1,4-dihydronicotinamide adenine dinucleotide: I186 (= I184), G189 (= G187), V190 (≠ Y188), I191 (= I189), E194 (= E192), E210 (= E208), A211 (= A209), L212 (= L210), A275 (= A276), V276 (≠ T277), G277 (= G278), R278 (= R279), M324 (= M330), L325 (= L331), V355 (≠ A361), Y357 (≠ F363)

Sites not aligning to the query:

active site: 473

5u8vA Dihydrolipoamide dehydrogenase (lpdg) from pseudomonas aeruginosa bound to NAD+ (see paper)
38% identity, 97% coverage: 7:470/479 of query aligns to 3:459/472 of 5u8vA

query
sites
5u8vA

Y

F

D

D

V

V

I

V

V

V

I
|
I

G

G

A

A

G
|
G

Y
x
P

G
|
G

G

G

F

Y

D

V

A

A

K

I

H

R

A

A

C

A

E

Q

K

L

G

G

L

L

K

K

T

T

A

A

I

C

V

I

E
|
E

A

K

R

Y

-

I

-

G

-

K

-

E

-

G

-

K

-

V

D

A

L
|
L

G

G

G
|
G

T
|
T

C
|
C

V

L

N

N

R

V

G
|
G

C
|
C

V

I

P

P

S
|
S

K
|
K

A

A

L

L

A

D

A

S

S

S

G

Y

R

K

V

Y

R

H

E

E

I

A

T

K

D

E

T

A

D

-

H

-

L

F

Q

K

N

V

F

H

G

G

I

I

Q

E

V

A

Q

K

G
|
G

V
|
V

S

T

F

I

D

D

R

V

Q

P

A

A

I

M

A

V

D

A

H

R

A

K

A

A

N

N

L

I

V

V

D

K

T

N

V

L

R

T

T

G

N

G

L

I

G

A

N

T

T

L

L

F

K

K

R

A

L

N

G

G

A

V

E

T

I

S

L

F

M

E

G

G

R
x
H

G
|
G

R

K

L

L

A

L

G

A

S

N

Q

K

R

Q

V

V

T

E

V

V

T

T

A

G

A

L

D

D

G

G

T

K

E

T

K

Q

T

V

Y

L

S

E

A

A

R

E

D

N

V

V

I

I

L

I

A
|
A

T
x
S

G
|
G

S

S

D

R

P

P

F

V

V

E

P

I

P

P

G

P

I

A

E

P

I

L

D

T

G

D

K

D

T

I

V

I

F

V

T

D

S
|
S

D

T

D

G

A

A

L

L

K

E

L

F

E

Q

T

A

L

V

P

P

Q

K

W

K

I

L

A

G

I

V

I
|
I

G
|
G

S

A

G
|
G

Y
x
V

I
|
I

G

G

L

L

E
|
E

F

L

S

G

D

S

V

V

Y

W

T

A

A

R

L

L

G

G

C

A

E

E

V

V

T

T

M

V

I
x
L

E
|
E

A
|
A

L

L

D

D

R

K

L

F

L

L

P

P

T

A

F

A

D

D

P

E

D

Q

V

I

A

A

K

K

V

E

A

A

Q

L

R

K

S

V

L

L

L

T

-

K

D

Q

G

G

R

L

D

N

I

I

E

R

T

L

R

G

T

A

G

R

V
|
V

L

T

A

A

R

S

K

E

I

V

T

K

P

K

G

K

S

Q

-

V

P

T

V

V

V

T

I

F

E

T

L

D

A

A

D

N

F

G

E

E

T

Q

K

K

E

E

L

-

V

-

E

-

V

-

L

-

E

T

V

F

D

D

A

K

C

L

L

I

V

V

A

A

T
x
V

G
|
G

R
|
R

I

R

P

P

S

V

T

T

K

T

N

D

L

L

G

L

L

A

E

A

T

D

V

S

A

G

V

V

E

T

V

L

D

D

R

E

R

R

G

G

F

F

I

I

P

Y

V

V

D

D

G

H

M

C

R

K

V

T

L

-

R

-

D

-

S

-

K

-

P

S

V

V

P

P

H

G

L

V

F

F

A

A

V

I

G
|
G

D
|
D

A

V

T

V

G

R

K

G

L

A

M
|
M

L
|
L

A
|
A

H
|
H

A

K

A

A

A
x
S

A

E

Q

E

G

G

V

V

V

M

A

V

I

A

E

E

N

R

I

I

T

A

G

G

H

H

P

K

R

A

S

Q

V

M

D

N

Y

Y

R

D

S

L

I

I

P

P

A

S

A

V

T

I

F

Y

T

T

H

H

P

P

E

E

I

I

S

A

S

W

V

V

G

G

L

K

S

T

E

E

A

Q

D

T

A

L

K

K

A

A

L

-

G

-

Q

-

E

E

G

G

F

V

E

E

V

V

G

N

S

V

V

G

R

T

S

F

Y

P

F

F

K

A

A

A

N

S

S

G

K

R

A

A

L

M

A

A

E

A

A

N

D

D

A

T

D

T

G

G

F

L

A

V

K

K

V

V

L

I

F

A

R

D

K

A

D

K

T

T

G

D

E

R

V

V

L

L

G

G

A

V

H

H

I

V

F

I

G

G

L

P

H

S

A

A

D

E

L

L

I

V

Q

Q

E

Q

V

G

A

A

N

I

A

G

I

M

A

E

R

F

R

G

Q

T

S

S

V

A

K

E

E

D

L

L

A

G

F

M

E

M

V

V

H
x
F

T

S

H
|
H

P
|
P

T

T

L

L

S

S

E
|
E

V

A

I

L

E

H

S

E

A

A

active site: P12 (≠ Y16), L43 (= L40), C47 (= C44), C52 (= C49), S55 (= S52), G81 (= G80), V82 (= V81), V189 (≠ Y188), E193 (= E192), S329 (≠ A336), F447 (≠ H458), H449 (= H460), E454 (= E465)
binding flavin-adenine dinucleotide: I8 (= I12), G11 (= G15), P12 (≠ Y16), G13 (= G17), E32 (= E36), G45 (= G42), T46 (= T43), C47 (= C44), G51 (= G48), C52 (= C49), K56 (= K53), H119 (≠ R118), G120 (= G119), A148 (= A147), S149 (≠ T148), G150 (= G149), S169 (= S168), I190 (= I189), R277 (= R279), G316 (= G323), D317 (= D324), M323 (= M330), L324 (= L331), A325 (= A332), H326 (= H333), H449 (= H460), P450 (= P461)
binding nicotinamide-adenine-dinucleotide: I185 (= I184), G186 (= G185), G188 (= G187), V189 (≠ Y188), I190 (= I189), L208 (≠ I207), E209 (= E208), A210 (= A209), V243 (= V241), V275 (≠ T277), G276 (= G278)

Sites not aligning to the query:

active site: 472

5u8uD Dihydrolipoamide dehydrogenase (lpdg) from pseudomonas aeruginosa (see paper)
38% identity, 97% coverage: 7:470/479 of query aligns to 7:463/477 of 5u8uD

query
sites
5u8uD

Y

F

D

D

V

V

I

V

V

V

I
|
I

G

G

A

A

G
|
G

Y
x
P

G
|
G

G

G

F

Y

D

V

A

A

K

I

H

R

A

A

C

A

E

Q

K

L

G

G

L

L

K

K

T

T

A

A

I

C

V

I

E
|
E

A
x
K

R

Y

-

I

-

G

-

K

-

E

-

G

-

K

-

V

D

A

L
|
L

G

G

G
|
G

T
|
T

C
|
C

V

L

N

N

R

V

G
|
G

C
|
C

V

I

P

P

S
|
S

K
|
K

A

A

L

L

A

D

A

S

S

S

G

Y

R

K

V

Y

R

H

E

E

I

A

T

K

D

E

T

A

D

-

H

-

L

F

Q

K

N

V

F

H

G

G

I

I

Q

E

V

A

Q

K

G
|
G

V
|
V

S

T

F

I

D

D

R

V

Q

P

A

A

I

M

A

V

D

A

H

R

A

K

A

A

N

N

L

I

V

V

D

K

T

N

V

L

R

T

T

G

N

G

L

I

G

A

N

T

T

L

L

F

K

K

R

A

L

N

G

G

A

V

E

T

I

S

L

F

M

E

G

G

R
x
H

G
|
G

R

K

L

L

A

L

G

A

S

N

Q

K

R

Q

V

V

T

E

V

V

T

T

A

G

A

L

D

D

G

G

T

K

E

T

K

Q

T

V

Y

L

S

E

A

A

R

E

D

N

V

V

I

I

L

I

A
|
A

T
x
S

G
|
G

S

S

D

R

P

P

F

V

V

E

P

I

P

P

G

P

I

A

E

P

I

L

D

T

G

D

K

D

T

I

V

I

F

V

T

D

S

S

D

T

D

G

A

A

L

L

K

E

L

F

E

Q

T

A

L

V

P

P

Q

K

W

K

I

L

A

G

I

V

I

I

G

G

S

A

G

G

Y
x
V

I
|
I

G

G

L

L

E
|
E

F

L

S

G

D

S

V

V

Y

W

T

A

A

R

L

L

G

G

C

A

E

E

V

V

T

T

M

V

I

L

E

E

A

A

L

L

D

D

R

K

L

F

L

L

P

P

T

A

F

A

D

D

P

E

D

Q

V

I

A

A

K

K

V

E

A

A

Q

L

R

K

S

V

L

L

L

T

-

K

D

Q

G

G

R

L

D

N

I

I

E

R

T

L

R

G

T

A

G

R

V

V

L

T

A

A

R

S

K

E

I

V

T

K

P

K

G

K

S

Q

-

V

P

T

V

V

V

T

I

F

E

T

L

D

A

A

D

N

F

G

E

E

T

Q

K

K

E

E

L

-

V

-

E

-

V

-

L

-

E

T

V

F

D

D

A

K

C

L

L

I

V

V

A

A

T

V

G

G

R
|
R

I

R

P

P

S

V

T

T

K

T

N

D

L

L

G

L

L

A

E

A

T

D

V

S

A

G

V

V

E

T

V

L

D

D

R

E

R

R

G

G

F

F

I

I

P

Y

V

V

D

D

G

H

M

C

R

K

V

T

L

-

R

-

D

-

S

-

K

-

P

S

V

V

P

P

H

G

L

V

F

F

A

A

V

I

G
|
G

D
|
D

A

V

T

V

G

R

K

G

L

A

M
|
M

L
|
L

A
|
A

H
|
H

A

K

A

A

A
x
S

A

E

Q

E

G

G

V

V

V

M

A

V

I

A

E

E

N

R

I

I

T

A

G

G

H

H

P

K

R

A

S

Q

V

M

D

N

Y

Y

R

D

S

L

I

I

P

P

A

S

A

V

T

I

F

Y

T

T

H

H

P

P

E

E

I

I

S

A

S

W

V

V

G

G

L

K

S

T

E

E

A

Q

D

T

A

L

K

K

A

A

L

-

G

-

Q

-

E

E

G

G

F

V

E

E

V

V

G

N

S

V

V

G

R

T

S

F

Y

P

F

F

K

A

A

A

N

S

S

G

K

R

A

A

L

M

A

A

E

A

A

N

D

D

A

T

D

T

G

G

F

L

A

V

K

K

V

V

L

I

F

A

R

D

K

A

D

K

T

T

G

D

E

R

V

V

L

L

G

G

A

V

H

H

I

V

F

I

G

G

L

P

H

S

A

A

D

E

L

L

I

V

Q

Q

E

Q

V

G

A

A

N

I

A

G

I

M

A

E

R

F

R

G

Q

T

S

S

V

A

K

E

E

D

L

L

A

G

F

M

E

M

V

V

H
x
F

T

S

H
|
H

P
|
P

T

T

L

L

S

S

E
|
E

V

A

I

L

E

H

S

E

A

A

active site: P16 (≠ Y16), L47 (= L40), C51 (= C44), C56 (= C49), S59 (= S52), G85 (= G80), V86 (= V81), V193 (≠ Y188), E197 (= E192), S333 (≠ A336), F451 (≠ H458), H453 (= H460), E458 (= E465)
binding flavin-adenine dinucleotide: I12 (= I12), G15 (= G15), P16 (≠ Y16), G17 (= G17), E36 (= E36), K37 (≠ A37), G49 (= G42), T50 (= T43), C51 (= C44), G55 (= G48), C56 (= C49), K60 (= K53), H123 (≠ R118), G124 (= G119), A152 (= A147), S153 (≠ T148), G154 (= G149), I194 (= I189), R281 (= R279), G320 (= G323), D321 (= D324), M327 (= M330), L328 (= L331), A329 (= A332), H330 (= H333), H453 (= H460), P454 (= P461)

Sites not aligning to the query:

active site: 476, 477

1zmdA Crystal structure of human dihydrolipoamide dehydrogenase complexed to nadh (see paper)
37% identity, 97% coverage: 5:470/479 of query aligns to 3:460/472 of 1zmdA

query
sites
1zmdA

F

I

D

D

Y

A

D

D

V

V

I

T

V

V

I
|
I

G
|
G

A

S

G
|
G

Y
x
P

G
|
G

G

G

F

Y

D

V

A

A

K

I

H

K

A

A

C

A

E

Q

K

L

G

G

L

F

K

K

T

T

A

V

I

C

V

I

E
|
E

A
x
K

R
x
N

D

E

-

T

L
|
L

G

G

G
|
G

T
|
T

C
|
C

V

L

N

N

R

V

G
|
G

C
|
C

V

I

P

P

S
|
S

K
|
K

A

A

L

L

A

N

S

S

G

H

R

Y

V

Y

R

H

E

M

I

A

T

H

D

G

T

T

D

D

H

-

L

F

Q

A

N

S

F

R

G

G

I

I

Q

E

V

M

Q

S

G

E

V

V

S

R

F

L

D

N

R

L

Q

D

A

K

I

M

A

M

D

E

H

Q

A

K

A

S

N

T

L

A

V

V

D

K

T

A

V

L

R

T

T

G

N

G

L

I

G

A

N

H

T

L

L

F

K

K

R

Q

L

N

G

K

A

V

E

V

I

H

L

V

M

N

G

G

R
x
Y

G
|
G

R

K

L

I

A

T

G

G

S

K

Q

N

R

Q

V

V

T

T

V

A

T

T

A

K

A

A

D

D

G

G

T

G

E

T

K

Q

T

V

Y

I

S

D

A

T

R

K

D

N

V

I

I

L

L

I

A

A

T
|
T

G
|
G

S

S

D

E

-

V

-

T

P

P

F

F

V

-

P

-

P

P

G

G

I

I

E

T

I

I

D

D

G

E

K

D

T

T

V

I

F

V

T

S

S
|
S

D

T

D

G

A

A

L

L

K

S

L

L

E

K

T

K

L

V

P

P

Q

E

W

K

I

M

A

V

I

V

I
|
I

G
|
G

S

A

G
|
G

Y
x
V

I
|
I

G

G

L

V

E
|
E

F

L

S

G

D

S

V

V

Y

W

T

Q

A

R

L

L

G

G

C

A

E

D

V

V

T

T

M

A

I

V

E
|
E

A
x
F

L
|
L

D

G

R

H

L

V

L

G

P

G

T

V

-

G

F

I

D

D

P

M

D

E

V

I

A

S

K

K

V

N

A

F

Q

Q

R

R

S

I

L

L

L

Q

-

K

D

Q

G

G

R

F

D

K

I

F

E

K

T

L

R

N

T

T

G

K

V

V

L

T

-

G

A

A

R

T

K

K

I

K

T

S

P

D

G

G

S

K

-

I

P

D

V

V

V

S

I

I

E

E

L

A

A

A

D

-

F

-

E

-

T

S

K

G

E

G

L

K

V

A

E

E

V

V

L

I

E

T

V

C

D

D

A

V

C

L

L

L

V

V

A

C

T
x
I

G
|
G

R
|
R

I

R

P

P

S
x
F

T

T

K

K

N

N

L

L

G

G

L

L

E

E

T

E

V

L

A

G

V

I

E

E

V

L

D

D

R

P

R

R

G

G

F

R

I

I

P

P

V

V

D

N

D

T

G

-

M

-

R

-

V

-

L

-

R

R

D

F

S

Q

K

T

P

K

V

I

P

P

H

N

L

I

F

Y

A

A

V

I

G
|
G

D
|
D

A

V

T

V

G

A

K

G

L

P

M
|
M

L
|
L

A
|
A

H
|
H

A

K

A

A

A

E

A

D

Q

E

G

G

V

I

A

C

I

V

E

E

N

G

I

M

T

A

G

G

H

G

P

A

R

V

S

H

V

I

D

D

Y

Y

R

N

S

C

I

V

P

P

A

S

A
x
V

T

I

F
x
Y

T

T

H

H

P

P

E

E

I

V

S

A

S

W

V

V

G

G

L

K

S

S

E

E

A

E

D

Q

A

L

K

K

A

-

L

-

G

-

Q

E

E

E

G

G

F

I

E

E

V

Y

G

K

S

V

V

G

R

K

S

F

Y

P

F

F

K

A

A

A

N

N

S

S

K

R

A

A

L

K

A

T

E

N

A

A

D

D

A

T

D

D

G

G

F

M

A

V

K

K

V

I

L

L

F

G

R

Q

K

K

D

S

T

T

G

D

E

R

V

V

L

L

G

G

A

A

H

H

I

I

F

L

G

G

L

P

H

G

A

A

A

G

D

E

L

M

I

V

Q

N

E

E

V

A

A

A

N

L

A

A

I

L

A

E

R

Y

R

G

Q

A

S

S

V

C

K

E

E

D

L

I

A

A

F

R

E

V

V

C

H
|
H

T

A

H
|
H

P

P

T

T

L

L

S

S

E
|
E

V

A

I

F

E

R

S

E

A

A

active site: L39 (= L40), C43 (= C44), C48 (= C49), S51 (= S52), V186 (≠ Y188), E190 (= E192), H448 (= H458), H450 (= H460), E455 (= E465)
binding flavin-adenine dinucleotide: I10 (= I12), G11 (= G13), G13 (= G15), P14 (≠ Y16), G15 (= G17), E34 (= E36), K35 (≠ A37), N36 (≠ R38), G41 (= G42), T42 (= T43), C43 (= C44), G47 (= G48), C48 (= C49), K52 (= K53), Y116 (≠ R118), G117 (= G119), T146 (= T148), G147 (= G149), S166 (= S168), R278 (= R279), F281 (≠ S282), G317 (= G323), D318 (= D324), M324 (= M330), L325 (= L331), A326 (= A332), H327 (= H333)
binding 1,4-dihydronicotinamide adenine dinucleotide: I182 (= I184), G183 (= G185), G185 (= G187), V186 (≠ Y188), I187 (= I189), E190 (= E192), E206 (= E208), F207 (≠ A209), L208 (= L210), I276 (≠ T277), G277 (= G278), R278 (= R279), M324 (= M330), L325 (= L331), V355 (≠ A361), Y357 (≠ F363)

1zmcA Crystal structure of human dihydrolipoamide dehydrogenase complexed to NAD+ (see paper)
37% identity, 97% coverage: 5:470/479 of query aligns to 3:460/472 of 1zmcA

query
sites
1zmcA

F

I

D

D

Y

A

D

D

V

V

I

T

V

V

I
|
I

G
|
G

A

S

G
|
G

Y
x
P

G
|
G

G

G

F

Y

D

V

A

A

K

I

H

K

A

A

C

A

E

Q

K

L

G

G

L

F

K

K

T

T

A

V

I

C

V

I

E
|
E

A
x
K

R
x
N

D

E

-

T

L
|
L

G

G

G
|
G

T
|
T

C
|
C

V

L

N

N

R

V

G
|
G

C
|
C

V

I

P

P

S
|
S

K
|
K

A

A

L

L

A

N

S

S

G

H

R

Y

V

Y

R

H

E

M

I

A

T

H

D

G

T

T

D

D

H

-

L

F

Q

A

N

S

F

R

G

G

I

I

Q

E

V

M

Q

S

G

E

V

V

S

R

F

L

D

N

R

L

Q

D

A

K

I

M

A

M

D

E

H

Q

A

K

A

S

N

T

L

A

V

V

D

K

T

A

V

L

R

T

T

G

N

G

L

I

G

A

N

H

T

L

L

F

K

K

R

Q

L

N

G

K

A

V

E

V

I

H

L

V

M

N

G

G

R
x
Y

G
|
G

R

K

L

I

A

T

G

G

S

K

Q

N

R

Q

V

V

T

T

V

A

T

T

A

K

A

A

D

D

G

G

T

G

E

T

K

Q

T

V

Y

I

S

D

A

T

R

K

D

N

V

I

I

L

L

I

A

A

T
|
T

G
|
G

S

S

D

E

-

V

-

T

P

P

F

F

V

-

P

-

P

P

G

G

I

I

E

T

I

I

D

D

G

E

K

D

T

T

V

I

F

V

T

S

S
|
S

D

T

D

G

A

A

L

L

K

S

L

L

E

K

T

K

L

V

P

P

Q

E

W

K

I

M

A

V

I

V

I

I

G
|
G

S

A

G
|
G

Y
x
V

I
|
I

G

G

L

V

E
|
E

F

L

S

G

D

S

V

V

Y

W

T

Q

A

R

L

L

G

G

C

A

E

D

V

V

T

T

M

A

I
x
V

E
|
E

A
x
F

L
|
L

D

G

R

H

L

V

L

G

P

G

T

V

-

G

F

I

D

D

P

M

D

E

V

I

A

S

K

K

V

N

A

F

Q

Q

R

R

S

I

L

L

L

Q

-

K

D

Q

G

G

R

F

D

K

I

F

E

K

T

L

R

N

T

T

G
x
K

V
|
V

L

T

-

G

A

A

R

T

K

K

I

K

T

S

P

D

G

G

S

K

-

I

P

D

V

V

V

S

I

I

E

E

L

A

A

A

D

-

F

-

E

-

T

S

K

G

E

G

L

K

V

A

E

E

V

V

L

I

E

T

V

C

D

D

A

V

C

L

L

L

V

V

A

C

T
x
I

G
|
G

R
|
R

I

R

P

P

S
x
F

T

T

K

K

N

N

L

L

G

G

L

L

E

E

T

E

V

L

A

G

V

I

E

E

V

L

D

D

R

P

R
|
R

G

G

F

R

I

I

P

P

V

V

D

N

D

T

G

-

M

-

R

-

V

-

L

-

R

R

D

F

S

Q

K

T

P

K

V

I

P

P

H

N

L

I

F

Y

A

A

V

I

G
|
G

D
|
D

A

V

T

V

G

A

K

G

L

P

M
|
M

L
|
L

A
|
A

H
|
H

A

K

A

A

A

E

A

D

Q

E

G

G

V

I

A

C

I

V

E

E

N

G

I

M

T

A

G

G

H

G

P

A

R

V

S

H

V

I

D

D

Y

Y

R

N

S

C

I

V

P

P

A

S

A

V

T

I

F

Y

T

T

H

H

P

P

E

E

I

V

S

A

S

W

V

V

G

G

L

K

S

S

E

E

A

E

D

Q

A

L

K

K

A

-

L

-

G

-

Q

E

E

E

G

G

F

I

E

E

V

Y

G

K

S

V

V

G

R

K

S

F

Y

P

F

F

K

A

A

A

N

N

S

S

K

R

A

A

L

K

A

T

E

N

A

A

D

D

A

T

D

D

G

G

F

M

A

V

K

K

V

I

L

L

F

G

R

Q

K

K

D

S

T

T

G

D

E

R

V

V

L

L

G

G

A

A

H

H

I

I

F

L

G

G

L

P

H

G

A

A

A

G

D

E

L

M

I

V

Q

N

E

E

V

A

A

A

N

L

A

A

I

L

A

E

R

Y

R

G

Q

A

S

S

V

C

K

E

E

D

L

I

A

A

F

R

E

V

V

C

H
|
H

T

A

H
|
H

P

P

T

T

L

L

S

S

E
|
E

V

A

I

F

E

R

S

E

A

A

active site: L39 (= L40), C43 (= C44), C48 (= C49), S51 (= S52), V186 (≠ Y188), E190 (= E192), H448 (= H458), H450 (= H460), E455 (= E465)
binding flavin-adenine dinucleotide: I10 (= I12), G11 (= G13), G13 (= G15), P14 (≠ Y16), G15 (= G17), E34 (= E36), K35 (≠ A37), N36 (≠ R38), G41 (= G42), T42 (= T43), C43 (= C44), G47 (= G48), C48 (= C49), K52 (= K53), Y116 (≠ R118), G117 (= G119), T146 (= T148), G147 (= G149), S166 (= S168), I187 (= I189), F281 (≠ S282), G317 (= G323), D318 (= D324), M324 (= M330), L325 (= L331), A326 (= A332), H327 (= H333)
binding nicotinamide-adenine-dinucleotide: G183 (= G185), G185 (= G187), V205 (≠ I207), E206 (= E208), F207 (≠ A209), L208 (= L210), K240 (≠ G240), V241 (= V241), I276 (≠ T277), G277 (= G278), R278 (= R279), R297 (= R298), M324 (= M330)

P09622 Dihydrolipoyl dehydrogenase, mitochondrial; Dihydrolipoamide dehydrogenase; Glycine cleavage system L protein; EC 1.8.1.4 from Homo sapiens (Human) (see 14 papers)
37% identity, 97% coverage: 5:470/479 of query aligns to 40:497/509 of P09622

query
sites
P09622

F

I

D

D

Y

A

D

D

V

V

I

T

V

V

I

I

G

G

A

S

G

G

Y

P

G

G

F

Y

D

V

A

A

K

I

H

K

A

A

C

A

E

Q

K

L

G

G

L

F

K

K

T

T

A

V

I

C

V

I

E
|
E

A
x
K

R
x
N

D
x
E

-
x
T

L
|
L

G
|
G

T
|
T

C
|
C

V

L

N

N

R

V

G

G

C

C

V

I

P

P

S

S

K
|
K

A

A

L

L

A

N

S

S

G

-

R

H

V

Y

R

Y

E

H

I

M

T

A

D

H

T

G

D
x
K

H

D

L

F

Q

A

N

S

F

R

G

G

I

I

Q

E

V

M

Q

S

G

E

V

V

S

R

F

L

D

N

R

L

Q

D

A

K

I

M

A

M

D

E

H

Q

A

K

A

S

N

T

L

A

V

V

D

K

T

A

V

L

R

T

T

G

N

G

L

I

G

A

N

H

T

L

L

F

K

K

R

Q

L

N

G

K

A

V

E

V

I

H

L

V

M

N

G

G

R

Y

G
|
G

R

K

L

I

A

T

G

G

S

K

Q

N

R

Q

V

V

T

T

V

A

T

T

A

K

A

A

D

D

G

G

T

G

E

T

K

Q

T

V

Y

I

S

D

A

T

R

K

D

N

V

I

I

L

L

I

A

A

T
|
T

G
|
G

S
|
S

D

E

-

V

-

T

P

P

F

F

V

-

P

-

P

P

G

G

I

I

E

T

I

I

D

D

G

E

K

D

T

T

V

I

F

V

T

S

S

S

D

T

D

G

A

A

L

L

K

S

L

L

E

K

T

K

L

V

P

P

Q

E

W

K

I

M

A

V

I

V

I

I

G
|
G

S
x
A

G
|
G

Y
x
V

I
|
I

G
|
G

L
x
V

E
|
E

F

L

S

G

D

S

V

V

Y

W

T

Q

A

R

L

L

G

G

C

A

E

D

V

V

T

T

M

A

I

V

E
|
E

A

F

L

L

D

G

R

H

L

V

L

G

P

G

T

V

-

G

F

I

D

D

P

M

D

E

V

I

A

S

K

K

V

N

A

F

Q

Q

R

R

S

I

L

L

L

Q

-

K

D

Q

G

G

R

F

D

K

I

F

E

K

T

L

R

N

T

T

G

K

V
|
V

L

T

-

G

A

A

R

T

K

K

I

K

T

S

P

D

G

G

S

K

-

I

P

D

V

V

V

S

I

I

E

E

L

A

A

A

D

-

F

-

E

-

T

S

K

G

E

G

L

K

V

A

E

E

V

V

L

I

E

T

V

C

D

D

A

V

C

L

L

L

V

V

A

C

T

I

G
|
G

R

R

I

R

P

P

S

F

T

T

K

K

N

N

L

L

G

G

L

L

E

E

T

E

V

L

A

G

V

I

E

E

V

L

D

D

R

P

R

R

G

G

F

R

I

I

P

P

V

V

D

N

D

T

G

-

M

-

R

-

V

-

L

-

R

R

D

F

S

Q

K

T

P

K

V

I

P

P

H

N

L

I

F

Y

A

A

V

I

G

G

D
|
D

A

V

T

V

G

A

K

G

L

P

M
|
M

L
|
L

A
|
A

H
|
H

A

K

A

A

A

E

A

D

Q

E

G

G

V

I

A

C

I

V

E
|
E

N

G

I

M

T

A

G

G

H

G

P

A

R

V

S
x
H

V

I

D

D

Y

Y

R

N

S

C

I

V

P

P

A

S

A

V

T

I

F

Y

T

T

H

H

P

P

E

E

I

V

S

A

S

W

V

V

G

G

L

K

S

S

E

E

A

E

D

Q

A

L

K

K

A

-

L

-

G

-

Q

E

E

E

G

G

F

I

E

E

V

Y

G

K

S

V

V

G

R

K

S

F

Y

P

F

F

K

A

A

A

N

N

S

S

K

R

A

A

L

K

A

T

E

N

A

A

D

D

A

T

D

D

G

G

F

M

A

V

K

K

V

I

L

L

F

G

R

Q

K

K

D

S

T

T

G
x
D

E

R

V

V

L

L

G

G

A

A

H

H

I

I

F

L

G

G

L

P

H

G

A

A

A

G

D

E

L

M

I

V

Q

N

E
|
E

V

A

A

A

N

L

A

A

I

L

A

E

R
x
Y

R

G

Q

A

S

S

V

C

K

E

E
x
D

L

I

A

A

F
x
R

E

V

V

C

H
|
H

T

A

H

H

P
|
P

T

T

L

L

S
|
S

E
|
E

V

A

I

F

E
x
R

S

E

A

A

71:80 (vs. 36:44, 60% identical) binding
K72 (≠ A37) to E: in DLDD; reduced dihydrolipoyl dehydrogenase activity; no effect on interaction with PDHX; dbSNP:rs121964987
K89 (= K53) binding ; mutation to E: Abolishes dihydrolipoyl dehydrogenase activity. Does not affect interaction with PDHX.
K104 (≠ D69) to T: in dbSNP:rs1130477
G154 (= G119) binding
TGS 183:185 (= TGS 148:150) binding
220:227 (vs. 185:192, 63% identical) binding
E243 (= E208) binding
V278 (= V241) binding
G314 (= G278) binding
D355 (= D324) binding
MLAH 361:364 (= MLAH 330:333) binding
E375 (= E344) to K: in DLDD; loss of enzyme activity; abolished interaction with PDHX; dbSNP:rs121964992
H383 (≠ S352) mutation to A: Reduces dihydrolipoyl dehydrogenase activity.; mutation to L: Reduces dihydrolipoyl dehydrogenase activity.
D448 (≠ G421) Important for interaction with PDHX and activity of multienzyme pyruvate dehydrogenase complex; mutation to A: Reduces interaction with PDHX. Inhibits multienzyme pyruvate dehydrogenase complex activity. Does not affect dihydrolipoyl dehydrogenase activity.; mutation to N: Does not affect dihydrolipoyl dehydrogenase activity.
E466 (= E439) mutation to A: Decreases dehydrogenase activity. Loss of proteolytic activity.
Y473 (≠ R446) Important for interaction with PDHX and activity of multienzyme pyruvate dehydrogenase complex; mutation to A: Reduces interaction with PDHX. Inhibits multienzyme pyruvate dehydrogenase complex activity. Does not affect dihydrolipoyl dehydrogenase activity.; mutation to F: Does not affect dihydrolipoyl dehydrogenase activity.; mutation to H: Reduces interaction with PDHX. Inhibits multienzyme pyruvate dehydrogenase complex activity. Does not affect dihydrolipoyl dehydrogenase activity.
D479 (≠ E452) to V: in DLDD; reduced dehydrogenase activity; increased proteolytic activity; dbSNP:rs397514649
R482 (≠ F455) to G: in DLDD; reduced enzyme activity; dbSNP:rs397514650; mutation to A: Does not affect dihydrolipoyl dehydrogenase activity.; mutation to M: Does not affect interaction with PDHX.
H485 (= H458) mutation to A: Loss of dehydrogenase activity. Increases proteolytic activity.
P488 (= P461) to L: in DLDD; no effect on interaction with PDHX; dbSNP:rs121964988
S491 (= S464) mutation to A: Loss of proteolytic activity. Does not affect dehydrogenase activity.
E492 (= E465) mutation to Q: Reduces dihydrolipoyl dehydrogenase activity. Does not affect interaction with PDHX.
R495 (≠ E468) to G: in DLDD; loss of enzyme activity; reduced interaction with PDHX; dbSNP:rs121964989

Sites not aligning to the query:

505 K→M: Reduces dihydrolipoyl dehydrogenase activity. Does not affect interaction with PDHX.

3ladA Refined crystal structure of lipoamide dehydrogenase from azotobacter vinelandii at 2.2 angstroms resolution. A comparison with the structure of glutathione reductase (see paper)
37% identity, 97% coverage: 7:470/479 of query aligns to 4:460/472 of 3ladA

query
sites
3ladA

Y

F

D

D

V

V

I

I

V

V

I
|
I

G
|
G

A

A

G
|
G

Y
x
P

G

G

F

Y

D

V

A

A

K

I

H

K

A

S

C

A

E

Q

K

L

G

G

L

L

K

K

T

T

A

A

I

L

V

I

E
|
E

A
x
K

R

Y

D

K

-

G

-

K

-

E

-

G

-

K

-

T

-

A

L
|
L

G

G

G
|
G

T
|
T

C
|
C

V

L

N

N

R

V

G
|
G

C
|
C

V

I

P

P

S
|
S

K

K

A

A

L

L

A

D

A

S

S

S

G

Y

R

K

V

F

R

H

E

E

I

A

T

H

D

E

T

S

D

F

H

K

L

L

Q

H

N

-

F

-

G

G

I

I

Q

S

V

T

Q

G

G

E

V

V

S

A

F

I

D

D

R

V

Q

P

A

T

I

M

A

I

D

A

H

R

A

K

A

D

N

Q

L

I

V

V

D

R

T

N

V

L

R

T

T

G

N

G

L

V

G

A

N

S

T

L

L

I

K

K

R

A

L

N

G

G

A

V

E

T

I

L

L

F

M

E

G

G

R
x
H

G
|
G

R

K

L

L

A

L

G

A

S

G

Q

K

R

K

V

V

T

E

V

V

T

T

A

A

D

D

G

G

T

S

E

S

K

Q

T

V

Y

L

S

D

A

T

R

E

D

N

V

V

I

I

L

L

A
|
A

T
x
S

G
|
G

S

S

D

K

P

P

F

V

V

E

P

I

P

P

G

P

I

A

E

P

I

V

D

D

G

Q

K

D

T

V

V

I

F

V

T

D

S

S

D

T

D

G

A

A

L

L

K

D

L

F

E

Q

T

N

L

V

P

P

Q

G

W

K

I

L

A

G

I

V

I

I

G

G

S

A

G

G

Y
x
V

I
|
I

G

G

L

L

E
|
E

F

L

S

G

D

S

V

V

Y

W

T

A

A

R

L

L

G

G

C

A

E

E

V

V

T

T

M

V

I

L

E

E

A

A

L

M

D

D

R

K

L

F

L

L

P

P

T

A

F

V

D

D

P

E

D

Q

V

V

A

A

K

K

V

E

A

A

Q

Q

R

K

S

I

L

L

L

-

D

-

G

-

R

-

D

-

I

-

E

-

T

T

R

K

T

Q

G

G

-

L

-

K

-

I

V

L

L

L

A

G

R

A

K

R

I

V

T

T

-

G

-

T

-

E

-

V

P

K

G

N

S

K

P

Q

V

V

V

T

I

V

E

K

L

F

A

V

D

D

F

A

E

E

T

G

K

E

E

-

L

-

V

-

E

K

V

S

L

Q

E

A

V

F

D

D

A

K

C

L

L

I

V

V

A

A

T

V

G

G

R
|
R

I

R

P

P

S

V

T

T

K

T

N

D

L

L

G

L

L

A

E

A

T

D

V

S

A

G

V

V

E

T

V

L

D

D

R

E

R

R

G

G

F

F

I

I

P

Y

V

V

D

D

G

-

M

-

R

-

V

-

L

-

R

Y

D

C

S

A

K

T

P

S

V

V

P

P

H

G

L

V

F

Y

A

A

V

I

G

G

D
|
D

A

V

T

V

G

R

K

G

L

A

M

M

L
|
L

A
|
A

H

H

A

K

A

A

A

S

A

E

Q

E

G

G

V

V

A

V

I

A

E

E

N

R

I

I

T

A

G

G

H

H

P

K

R

A

S

Q

V

M

D

N

Y

Y

R

D

S

L

I

I

P

P

A

A

A

V

T

I

F

Y

T

T

H

H

P

P

E

E

I

I

S

A

S

G

V

V

G

G

L

K

S

T

E

E

A

Q

D

A

A

L

K

K

A

A

L

-

G

-

Q

-

E

E

G

G

F

V

E

A

V

I

G

N

S

V

V

G

R

V

S

F

Y

P

F

F

K

A

A

A

N

S

S

G

K

R

A

A

L

M

A

A

E

A

A

N

D

D

A

T

D

A

G

G

F

F

A

V

K

K

V

V

L

I

F

A

R

D

K

A

D

K

T

T

G

D

E

R

V

V

L

L

G

G

A

V

H

H

I

V

F

I

G

G

L

P

H

S

A

A

D

E

L

L

I

V

Q

Q

E

Q

V

G

A

A

N

I

A

A

I

M

A

E

R

F

R

G

Q

T

S

S

V

A

K

E

E

D

L

L

A

G

F

M

E

M

V

V

H
x
F

T

A

H
|
H

P

P

T

A

L

L

S

S

E
|
E

V

A

I

L

E

H

S

E

A

A

active site: L44 (= L40), C48 (= C44), C53 (= C49), S56 (= S52), V190 (≠ Y188), E194 (= E192), F448 (≠ H458), H450 (= H460), E455 (= E465)
binding flavin-adenine dinucleotide: I9 (= I12), G10 (= G13), G12 (= G15), P13 (≠ Y16), E33 (= E36), K34 (≠ A37), G46 (= G42), T47 (= T43), C48 (= C44), G52 (= G48), C53 (= C49), H120 (≠ R118), G121 (= G119), A149 (= A147), S150 (≠ T148), G151 (= G149), I191 (= I189), R278 (= R279), D318 (= D324), L325 (= L331), A326 (= A332)

6hg8B Crystal structure of the r460g disease-causing mutant of the human dihydrolipoamide dehydrogenase.
37% identity, 97% coverage: 5:470/479 of query aligns to 13:470/482 of 6hg8B

query
sites
6hg8B

F

I

D

D

Y

A

D

D

V

V

I

T

V

V

I
|
I

G

G

A

S

G
|
G

Y
x
P

G
|
G

G

G

F

Y

D

V

A

A

K

I

H

K

A

A

C

A

E

Q

K

L

G

G

L

F

K

K

T

T

A

V

I

C

V

I

E
|
E

A
x
K

R
x
N

D

E

-

T

L

L

G

G

G
|
G

T
|
T

C
|
C

V

L

N

N

R

V

G
|
G

C
|
C

V

I

P

P

S
|
S

K
|
K

A

A

L

L

A

N

S

S

G

-

R

H

V

Y

R

Y

E

H

I

M

T

A

D

H

T

G

D

K

H

D

L

F

Q

A

N

S

F

R

G

G

I

I

Q

E

V

M

Q

S

G

E

V

V

S

R

F

L

D

N

R

L

Q

D

A

K

I

M

A

M

D

E

H

Q

A

K

A

S

N

T

L

A

V

V

D

K

T

A

V

L

R

T

T

G

N

G

L

I

G

A

N

H

T

L

L

F

K

K

R

Q

L

N

G

K

A

V

E

V

I

H

L

V

M

N

G

G

R
x
Y

G
|
G

R

K

L

I

A

T

G

G

S

K

Q

N

R

Q

V

V

T

T

V

A

T

T

A

K

A

A

D

D

G

G

T

G

E

T

K

Q

T

V

Y

I

S

D

A

T

R

K

D

N

V

I

I

L

L

I

A

A

T
|
T

G
|
G

S

S

D

E

-

V

-

T

P

P

F

F

V

-

P

-

P

P

G

G

I

I

E

T

I

I

D

D

G

E

K

D

T

T

V

I

F

V

T

S

S

S

D

T

D

G

A

A

L

L

K

S

L

L

E

K

T

K

L

V

P

P

Q

E

W

K

I

M

A

V

I

V

I

I

G

G

S

A

G

G

Y
x
V

I
|
I

G

G

L

V

E
|
E

F

L

S

G

D

S

V

V

Y

W

T

Q

A

R

L

L

G

G

C

A

E

D

V

V

T

T

M

A

I

V

E

E

A

F

L

L

D

G

R

H

L

V

L

G

P

G

T

V

-

G

F

I

D

D

P

M

D

E

V

I

A

S

K

K

V

N

A

F

Q

Q

R

R

S

I

L

L

L

Q

-

K

D

Q

G

G

R

F

D

K

I

F

E

K

T

L

R

N

T

T

G

K

V

V

L

T

-

G

A

A

R

T

K

K

I

K

T

S

P

D

G

G

S

K

-

I

P

D

V

V

V

S

I

I

E

E

L

A

A

A

D

-

F

-

E

-

T

S

K

G

E

G

L

K

V

A

E

E

V

V

L

I

E

T

V

C

D

D

A

V

C

L

L

L

V

V

A

C

T

I

G

G

R
|
R

I

R

P

P

S
x
F

T

T

K

K

N

N

L

L

G

G

L

L

E

E

T

E

V

L

A

G

V

I

E

E

V

L

D

D

R

P

R

R

G

G

F

R

I

I

P

P

V

V

D

N

D

T

G

-

M

-

R

-

V

-

L

-

R

R

D

F

S

Q

K

T

P

K

V

I

P

P

H

N

L

I

F

Y

A

A

V

I

G
|
G

D
|
D

A

V

T

V

G

A

K

G

L

P

M
|
M

L
|
L

A
|
A

H
|
H

A

K

A

A

A

E

A

D

Q

E

G

G

V

I

A

C

I

V

E

E

N

G

I

M

T

A

G

G

H

G

P

A

R

V

S

H

V

I

D

D

Y

Y

R

N

S

C

I

V

P

P

A

S

A

V

T

I

F

Y

T

T

H

H

P

P

E

E

I

V

S

A

S

W

V

V

G

G

L

K

S

S

E

E

A

E

D

Q

A

L

K

K

A

-

L

-

G

-

Q

E

E

E

G

G

F

I

E

E

V

Y

G

K

S

V

V

G

R

K

S

F

Y

P

F

F

K

A

A

A

N

N

S

S

K

R

A

A

L

K

A

T

E

N

A

A

D

D

A

T

D

D

G

G

F

M

A

V

K

K

V

I

L

L

F

G

R

Q

K

K

D

S

T

T

G

D

E

R

V

V

L

L

G

G

A

A

H

H

I

I

F

L

G

G

L

P

H

G

A

A

A

G

D

E

L

M

I

V

Q

N

E

E

V

A

A

A

N

L

A

A

I

L

A

E

R

Y

R

G

Q

A

S

S

V

C

K

E

E

D

L

I

A

A

F

R

E

V

V

C

H

H

T

A

H
|
H

P

P

T

T

L

L

S

S

E
|
E

V

A

I

F

E

G

S

E

A

A

active site: C53 (= C44), C58 (= C49), S61 (= S52), V196 (≠ Y188), E200 (= E192), H460 (= H460), E465 (= E465)
binding flavin-adenine dinucleotide: I20 (= I12), G23 (= G15), P24 (≠ Y16), G25 (= G17), E44 (= E36), K45 (≠ A37), N46 (≠ R38), G51 (= G42), T52 (= T43), C53 (= C44), G57 (= G48), C58 (= C49), K62 (= K53), Y126 (≠ R118), G127 (= G119), T156 (= T148), G157 (= G149), I197 (= I189), R288 (= R279), F291 (≠ S282), G327 (= G323), D328 (= D324), M334 (= M330), L335 (= L331), A336 (= A332), H337 (= H333)

P18925 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; EC 1.8.1.4 from Azotobacter vinelandii (see 2 papers)
37% identity, 97% coverage: 7:470/479 of query aligns to 5:461/477 of P18925

query
sites
P18925

Y

F

D

D

V

V

I

I

V

V

I

I

G

G

A

A

G

G

Y

P

G

G

F

Y

D

V

A

A

K

I

H

K

A

S

C

A

E

Q

K

L

G

G

L

L

K

K

T

T

A

A

I

L

V

I

E
|
E

A
x
K

R
x
Y

D
x
K

-
x
G

-
x
K

-
x
E

-
x
G

-
x
K

-
x
T

-
x
A

L
|
L

G
|
G

T
|
T

C
|
C

V

L

N

N

R

V

G

G

C
|
C

V

I

P

P

S

S

K
|
K

A

A

L

L

A

D

A

S

S

S

G

Y

R

K

V

F

R

H

E

E

I

A

T

H

D

E

T

S

D

F

H

K

L

L

Q

H

N

-

F

-

G

G

I

I

Q

S

V

T

Q

G

G

E

V

V

S

A

F

I

D

D

R

V

Q

P

A

T

I

M

A

I

D

A

H

R

A

K

A

D

N

Q

L

I

V

V

D

R

T

N

V

L

R

T

T

G

N

G

L

V

G

A

N

S

T

L

L

I

K

K

R

A

L

N

G

G

A

V

E

T

I

L

L

F

M

E

G

G

R

H

G

G

R

K

L

L

A

L

G

A

S

G

Q

K

R

K

V

V

T

E

V

V

T

T

A

A

D

D

G

G

T

S

E

S

K

Q

T

V

Y

L

S

D

A

T

R

E

D

N

V

V

I

I

L

L

A

A

T

S

G

G

S

S

D

K

P

P

F

V

V

E

P

I

P

P

G

P

I

A

E

P

I

V

D

D

G

Q

K

D

T

V

V

I

F

V

T

D

S

S

D

T

D

G

A

A

L

L

K

D

L

F

E

Q

T

N

L

V

P

P

Q

G

W

K

I

L

A

G

I

V

I

I

G

G

S

A

G

G

Y

V

I

I

G

G

L

L

E

E

F

L

S

G

D

S

V

V

Y

W

T

A

A

R

L

L

G

G

C

A

E

E

V

V

T

T

M

V

I

L

E

E

A

A

L

M

D

D

R

K

L

F

L

L

P

P

T

A

F

V

D

D

P

E

D

Q

V

V

A

A

K

K

V

E

A

A

Q

Q

R

K

S

I

L

L

L

-

D

-

G

-

R

-

D

-

I

-

E

-

T

T

R

K

T

Q

G

G

-

L

-

K

-

I

V

L

L

L

A

G

R

A

K

R

I

V

T

T

-

G

-

T

-

E

-

V

P

K

G

N

S

K

P

Q

V

V

V

T

I

V

E

K

L

F

A

V

D

D

F

A

E

E

T

G

K

E

E

-

L

-

V

-

E

K

V

S

L

Q

E

A

V

F

D

D

A

K

C

L

L

I

V

V

A

A

T

V

G

G

R

R

I

R

P

P

S

V

T

T

K

T

N

D

L

L

G

L

L

A

E

A

T

D

V

S

A

G

V

V

E

T

V

L

D

D

R

E

R

R

G

G

F

F

I

I

P

Y

V

V

D

D

G

-

M

-

R

-

V

-

L

-

R

Y

D

C

S

A

K

T

P

S

V

V

P

P

H

G

L

V

F

Y

A

A

V

I

G

G

D
|
D

A

V

T

V

G

R

K

G

L

A

M

M

L

L

A
|
A

H

H

A

K

A

A

A

S

A

E

Q

E

G

G

V

V

A

V

I

A

E

E

N

R

I

I

T

A

G

G

H

H

P

K

R

A

S

Q

V

M

D

N

Y

Y

R

D

S

L

I

I

P

P

A

A

A

V

T

I

F

Y

T

T

H

H

P

P

E

E

I

I

S

A

S

G

V

V

G

G

L

K

S

T

E

E

A

Q

D

A

A

L

K

K

A

A

L

-

G

-

Q

-

E

E

G

G

F

V

E

A

V

I

G

N

S

V

V

G

R

V

S

F

Y

P

F

F

K

A

A

A

N

S

S

G

K

R

A

A

L

M

A

A

E

A

A

N

D

D

A

T

D

A

G

G

F

F

A

V

K

K

V

V

L

I

F

A

R

D

K

A

D

K

T

T

G

D

E

R

V

V

L

L

G

G

A

V

H

H

I

V

F

I

G

G

L

P

H

S

A

A

D

E

L

L

I

V

Q

Q

E

Q

V

G

A

A

N

I

A

A

I

M

A

E

R

F

R

G

Q

T

S

S

V

A

K

E

E

D

L

L

A

G

F

M

E

M

V

V

H

F

T

A

H

H

P

P

T

A

L

L

S

S

E

E

V

A

I

L

E

H

S

E

A

A

34:49 (vs. 36:44, 38% identical) binding
C49 (= C44) modified: Disulfide link with 54, Redox-active
C54 (= C49) modified: Disulfide link with 49, Redox-active
K58 (= K53) binding
D319 (= D324) binding
A327 (= A332) binding

6uziC Crystal structure of dihydrolipoyl dehydrogenase from elizabethkingia anophelis nuhp1
35% identity, 97% coverage: 7:470/479 of query aligns to 7:458/470 of 6uziC

query
sites
6uziC

Y

F

D

D

V

V

I

A

V

V

I
|
I

G
|
G

A

S

G
|
G

Y
x
P

G
|
G

G

G

F

Y

D

V

A

A

K

I

H

R

A

C

C

A

E

Q

K

L

G

G

L

F

K

K

T

T

A

V

I

I

V

I

E
|
E

A
x
K

R

Y

D

S

-

T

L

L

G

G

G
|
G

T
|
T

C
|
C

V

L

N

N

R

V

G
|
G

C
|
C

V

I

P

P

S
|
S

K
|
K

A

A

L

L

A

D

A

S

S

S

G

E

R

H

V

F

R

E

E

N

I

A

T

K

D

H

T

T

D

-

H

-

L

F

Q

A

N

T

F

H

G

G

I

I

Q

L

V

I

Q

D

G

E

V

P

S

K

F

V

D

D

R

I

Q

A

A

Q

I

M

A

I

D

S

H

R

A

K

A

N

N

D

L

V

V

V

D

D

T

Q

V

T

R

T

T

K

N

G

L

I

G

N

N

F

T

L

L

M

K

D

R

K

L

N

G

K

A

I

E

T

I

V

L

L

M

Q

G

G

R
x
V

G
|
G

R

S

L

F

A

E

G

S

S

A

Q

T

R

Q

V

I

T

K

V

V

T

T

A

K

A

A

D

D

G

G

T

S

E

S

K

E

T

V

Y

I

S

E

A

A

R

K

D

N

V

T

I

I

L

I

A

A

T
|
T

G
|
G

S

S

D

K

P

P

F

S

V

S

P

L

P

P

G

F

I

I

E

T

I

L

D

D

G

K

K

E

T

R

V

V

F

I

T

T

S

S

D

T

D

E

A

A

L

L

K

N

L

L

E

K

T

E

L

V

P

P

Q

K

W

H

I

L

A

I

I

V

I

I

G

G

S

G

G

G

Y
x
V

I
|
I

G

G

L

L

E
|
E

F

L

S

G

D

S

V

V

Y

Y

T

L

A

R

L

L

G

G

C

S

E

D

V

V

T

T

M

V

I

V

E

E

A

Y

L

L

D

D

R

K

L

I

P

P

T

G

F

M

D

D

P

G

D

T

V

L

A

S

K

K

V

E

A

L

Q

Q

R

K

S

T

L

L

-

K

L

K

D

Q

G

G

R

M

D

K

I

F

E

M

T

L

R

S

T

T

G

A

V

V

L

S

A

G

R

V

K

E

I

-

T

R

P

N

G

G

S

D

P

T

V

V

V

K

I

V

E

T

L

A

A

K

D

D

F

K

E

K

T

G

K

E

E

D

L

V

V

-

E

-

V

V

L

V

E

E

V

G

D

D

A

Y

C

C

L

L

V

V

A

S

T

V

G

G

R
|
R

I

R

P

P

S

Y

T

T

K

D

N

G

L

L

G

G

L

L

E

E

T

K

V

A

A

G

V

V

E

E

V

L

D

D

R

E

R

R

G

G

F

R

I

V

P

K

V

T

D

N

D

D

G

H

M

L

R

Q

V

T

L

-

R

-

D

-

S

-

K

-

P

N

V

V

P

P

H

N

L

I

F

Y

A

A

V

I

G

G

D
|
D

A

V

T

V

G

K

K

G

L

A

M
|
M

L
|
L

A
|
A

H

H

A

K

A

A

A

E

Q

E

G

G

V

V

V

F

A

V

I

A

E

E

N

T

I

L

T

A

G

G

H

E

P

K

R

P

S

H

V

V

D

N

Y

Y

R

N

S

L

I

I

P

P

A

G

A

V

T

V

F

Y

T

T

H

W

P

P

E

E

I

V

S

A

S

G

V

V

G

G

L

K

S

T

E

E

A

E

D

Q

A

L

K

K

A

E

L

A

G

G

G

-

Q

-

E

V

G

A

F

Y

E

K

V

T

G

G

S

S

V

F

R

P

S

-

Y

-

F

M

K

R

A

A

N

L

S

G

K

R

A

S

L

R

A

A

E

S

A

M

D

D

A

T

D

D

G

G

F

V

A

I

K

K

V

I

L

L

F

A

R

D

K

E

D

K

T

T

G

D

E

E

V

I

L

L

G

G

A

V

H

H

I

M

F

I

G

G

L

A

H

R

A

A

D

D

L

M

I

I

Q

A

E

E

V

A

A

V

N

V

A

A

I

M

A

E

R

F

R

R

Q

A

S

S

V

A

K

E

E

D

L

I

A

A

F

R

E

I

V

S

H

H

T

A

H
|
H

P

P

T

T

L

Y

S

T

E
|
E

V

A

I

I

E

K

S

E

A

A

active site: C45 (= C44), C50 (= C49), S53 (= S52), V187 (≠ Y188), E191 (= E192), H448 (= H460), E453 (= E465)
binding flavin-adenine dinucleotide: I12 (= I12), G13 (= G13), G15 (= G15), P16 (≠ Y16), G17 (= G17), E36 (= E36), K37 (≠ A37), G43 (= G42), T44 (= T43), C45 (= C44), G49 (= G48), C50 (= C49), S53 (= S52), K54 (= K53), V117 (≠ R118), G118 (= G119), T147 (= T148), G148 (= G149), I188 (= I189), R276 (= R279), D316 (= D324), M322 (= M330), L323 (= L331), A324 (= A332)
binding zinc ion: H448 (= H460), E453 (= E465)

3urhB Crystal structure of a dihydrolipoamide dehydrogenase from sinorhizobium meliloti 1021
36% identity, 97% coverage: 7:470/479 of query aligns to 1:455/465 of 3urhB

query
sites
3urhB

Y

Y

D

D

V

L

I

I

V

V

I
|
I

G
|
G

A

S

G
|
G

Y
x
P

G
|
G

G

G

F

Y

D

V

A

C

A

A

K

I

H

K

A

A

C

A

E

Q

K

L

G

G

L

M

K

K

T

V

A

A

I

V

V

V

E
|
E

A
x
K

R

R

D

S

-

T

L
x
Y

G

G

G
|
G

T
|
T

C
|
C

V

L

N

N

R

V

G
|
G

C
|
C

V

I

P

P

S
|
S

K
|
K

A

A

L

L

A

H

A

A

S

S

G

E

R

M

V

F

R

H

E

Q

I

-

T

-

D

-

T

A

D

Q

H

H

-

G

L

L

Q

E

N

A

F

L

G

G

I

V

Q

E

V

V

Q

A

G

N

V

P

S

K

F

L

D

N

R

L

Q

Q

A

K

I

M

A

M

D

A

H

H

A

K

A

D

N

A

L

T

V

V

D

K

T

S

V

N

R

V

T

D

N

G

L

V

G

S

N

F

T

L

L

F

K

K

R

K

L

N

G

K

A

I

E

D

I

G

L

F

M

Q

G

G

R
x
T

G
|
G

R

K

L

V

A

L

G

G

S

Q

Q

G

R

K

V

V

T

S

V

V

T

T

A

N

A

E

D

K

G

G

T

E

E

E

K

Q

T

V

Y

L

S

E

A

A

R

K

D

N

V

V

I

V

L

I

A
|
A

T
|
T

G
|
G

S

S

D

D

P

V

F

A

V

G

P

I

P

P

G

G

I

V

E

E

I

V

-

A

-

F

D

D

G

E

K

K

T

T

V

I

F

V

T

S

S

S

D

T

D

G

A

A

L

L

K

A

L

L

E

E

T

K

L

V

P

P

Q

A

W

S

I

M

A

I

I

V

G

G

S

G

G

G

Y
x
V

I
|
I

G

G

L

L

E
|
E

F

L

S

G

D

S

V

V

Y

W

T

A

A

R

L

L

G

G

C

A

E

K

V

V

T

T

M

V

I

V

E

E

A

F

L

L

D

D

R

T

L

I

L

L

P

G

T

G

F

M

D

D

P

G

D

E

V

V

A

A

K

K

V

Q

A

L

Q

Q

R

R

S

-

L

M

L

L

D

T

G

K

R

Q

D

G

I

I

E

D

T

F

R

K

T

L

G

G

V

A

-

K

L

V

A

T

R

G

K

A

I

V

T

K

P

S

G

G

S

D

P

G

V

A

V

K

I

V

E

T

L

F

A

E

D

P

F

V

E

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A

active site: Y35 (≠ L40), C39 (= C44), C44 (= C49), S47 (= S52), V183 (≠ Y188), E187 (= E192), H443 (= H458), H445 (= H460), E450 (= E465)
binding flavin-adenine dinucleotide: I6 (= I12), G7 (= G13), G9 (= G15), P10 (≠ Y16), G11 (= G17), E30 (= E36), K31 (≠ A37), G37 (= G42), T38 (= T43), C39 (= C44), G43 (= G48), C44 (= C49), K48 (= K53), T111 (≠ R118), G112 (= G119), A140 (= A147), T141 (= T148), G142 (= G149), I184 (= I189), R273 (= R279), G312 (= G323), D313 (= D324), M319 (= M330), L320 (= L331), A321 (= A332), H322 (= H333)

P31023 Dihydrolipoyl dehydrogenase, mitochondrial; Dihydrolipoamide dehydrogenase; Glycine cleavage system L protein; Pyruvate dehydrogenase complex E3 subunit; E3; PDC-E3; EC 1.8.1.4 from Pisum sativum (Garden pea) (Lathyrus oleraceus) (see 2 papers)
35% identity, 97% coverage: 6:470/479 of query aligns to 37:490/501 of P31023

query
sites
P31023

D

E

Y

N

D

D

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V

I

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V

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I

I

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A

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x
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R

D
x
G

-
x
A

L
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L

G
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G

T
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T

C
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C

V

L

N

N

R

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G

G

C
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C

V

I

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P

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S

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K

A

A

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L

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H

A

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S

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M

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Y

R

H

E

E

I

A

T

K

D

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S

D

-

H

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L

F

Q

A

N

N

F

H

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G

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K

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Q

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G

N

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E

F

I

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D

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A

A

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A

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A

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K

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T

R

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L

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A

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A

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A

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67:76 (vs. 36:44, 70% identical) binding
C76 (= C44) modified: Disulfide link with 81, Redox-active
C81 (= C49) modified: Disulfide link with 76, Redox-active
G149 (= G119) binding
D348 (= D324) binding
MLAH 354:357 (= MLAH 330:333) binding

Sites not aligning to the query:

1:31 modified: transit peptide, Mitochondrion

1dxlA Dihydrolipoamide dehydrogenase of glycine decarboxylase from pisum sativum (see paper)
35% identity, 97% coverage: 6:470/479 of query aligns to 3:456/467 of 1dxlA

query
sites
1dxlA

D

E

Y

N

D

D

V

V

I

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V

I

I
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I

G

G

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G

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x
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A

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A

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E

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x
K

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G

-

A

L
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L

G

G

G
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G

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T

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L

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N

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K

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A

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L

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A

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R

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E

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D

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N

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A

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L

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L

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x
Y

G
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G

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R

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Y

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E

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E

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T

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D

P

A

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K

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A

F

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Q

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D

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L

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A

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R

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N

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G

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A

A

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N

S

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R

A

A

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K

A

A

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I

A

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N

A

A

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A

A

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I

A

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K

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A

active site: L38 (= L40), C42 (= C44), C47 (= C49), S50 (= S52), Y184 (= Y188), E188 (= E192), H444 (= H458), H446 (= H460), E451 (= E465)
binding flavin-adenine dinucleotide: I9 (= I12), P13 (≠ Y16), G14 (= G17), E33 (= E36), K34 (≠ A37), R35 (= R38), G40 (= G42), T41 (= T43), C42 (= C44), G46 (= G48), C47 (= C49), K51 (= K53), Y114 (≠ R118), G115 (= G119), T144 (= T148), G145 (= G149), Y184 (= Y188), I185 (= I189), R274 (= R279), D314 (= D324), M320 (= M330), L321 (= L331), A322 (= A332), H323 (= H333)

Query Sequence

>Synpcc7942_1198 FitnessBrowser__SynE:Synpcc7942_1198
VSSEFDYDVIVIGAGYGGFDAAKHACEKGLKTAIVEARDLGGTCVNRGCVPSKALLAASG
RVREITDTDHLQNFGIQVQGVSFDRQAIADHAANLVDTVRTNLGNTLKRLGAEILMGRGR
LAGSQRVTVTAADGTEKTYSARDVILATGSDPFVPPGIEIDGKTVFTSDDALKLETLPQW
IAIIGSGYIGLEFSDVYTALGCEVTMIEALDRLLPTFDPDVAKVAQRSLLDGRDIETRTG
VLARKITPGSPVVIELADFETKELVEVLEVDACLVATGRIPSTKNLGLETVAVEVDRRGF
IPVDDGMRVLRDSKPVPHLFAVGDATGKLMLAHAAAAQGVVAIENITGHPRSVDYRSIPA
ATFTHPEISSVGLSEADAKALGGQEGFEVGSVRSYFKANSKALAEADADGFAKVLFRKDT
GEVLGAHIFGLHAADLIQEVANAIARRQSVKELAFEVHTHPTLSEVIESAYKQAATAIA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory