SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Synpcc7942_1352 FitnessBrowser__SynE:Synpcc7942_1352 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
55% identity, 98% coverage: 13:652/656 of query aligns to 3:638/648 of Q89WV5

query
sites
Q89WV5

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G263 (= G273) mutation to I: Loss of activity.
G266 (= G276) mutation to I: Great decrease in activity.
K269 (= K279) mutation to G: Great decrease in activity.
E414 (= E424) mutation to Q: Great decrease in activity.

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
54% identity, 97% coverage: 20:654/656 of query aligns to 11:643/652 of P27550

query
sites
P27550

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K609 (= K621) modified: N6-acetyllysine; by autocatalysis

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
54% identity, 97% coverage: 20:654/656 of query aligns to 7:637/641 of 2p20A

query
sites
2p20A

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|
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x
V

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|
T

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Y

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|
P

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E

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|
D

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A

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D

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Y

Y

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Y

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W

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x
I

M

T

G

G

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|
R

V

V

D

D

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V

I

L

N
|
N

V

V

S

S

G

G

H

H

R
|
R

L

L

G

G

T

T

M

A

E

E

I

I

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A

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L

L

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L

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T

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G

K
|
K

I

I

M

M

R

R

L

I

L

L

R

R

S

K

L

I

A

A

G

G

Q

-

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V

T

S

S

G

N

D

D

T

T

S

S

T

T

L

L

E

A

D

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P

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V

V

L

V

D

E

K

K

L

L

R

L

Q

E

active site: T260 (= T271), T412 (= T423), E413 (= E424), N517 (= N533), R522 (= R538), K605 (= K621)
binding adenosine-5'-monophosphate-propyl ester: V306 (≠ I317), T307 (= T318), G383 (= G394), E384 (= E395), P385 (= P396), D407 (= D418), T408 (= T419), W409 (= W420), W410 (= W421), Q411 (= Q422), T412 (= T423), D496 (= D512), I508 (≠ V524), R511 (= R527), N517 (= N533), R522 (= R538)

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
54% identity, 97% coverage: 20:654/656 of query aligns to 11:643/652 of Q8ZKF6

query
sites
Q8ZKF6

A

A

E

N

F

I

A

A

S

D

A

R

A

C

R

L

I

I

N

N

P

P

E

E

A

Q

Y

Y

E

E

A

T

L

K

C

Y

Q

K

K

Q

A

S

A

I

A

N

D

D

P

P

V

D

A

T

F

F

W

W

G

G

E

E

L

-

A

Q

A

G

Q

K

E

I

L

L

D

D

W

W

F

I

E

T

P

P

W

Y

Q

Q

Q

K

T

V

L

K

D

N

W

T

S

S

N

F

P

A

P

P

-

G

-

N

-

V

F

S

A

I

K

K

W

W

F

Y

V

E

G

D

G

G

K

T

L

L

N

N

I

L

S

A

H

A

N

N

C

C

L

L

D

D

R

R

H

H

L

L

T

Q

T

E

W

-

R

N

K

G

N

D

K

R

A

T

A

A

I

I

W

W

E

E

G

G

E

D

-

D

P

T

G

S

D

Q

S

S

R

K

T

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L

I

T

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Y

Y

A

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Q

E

L

L

H

H

R

R

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D

V

V

C

C

Q

R

F

F

A

A

N

N

V

T

L

L

K

L

S

D

L

L

G

G

I

I

Q

K

K

K

G

G

D

D

V

V

G

A

V

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

A

A

I

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

S

S

V

V

V

I

F

F

G

G

F

F

S

S

A

P

E

E

A

A

L

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A

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I

D

D

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A

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L

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V

V

I

T

T

A

A

D

D

G

E

G

G

W

V

R

R

K

A

D

G

A
x
R

I

S

V

I

P

P

L

L

K

K

D

K

S

N

V

V

D

D

Q

D

A

A

L

L

E

K

G

N

N

P

A

N

C

V

P

T

S

S

V

V

Q

E

H

H

V

V

L

I

V

V

V

L

E

K

R

R

T

T

K

G

Q

S

D

D

I

I

H

D

M

W

E

Q

P

E

G

G

R

R

D

D

H

L

W

W

H

R

E

D

L

L

Q

I

Q

E

T

K

V

A

S

S

A

P

T

E

C

H

P

Q

A

P

E

E

P

A

M

M

D

N

S

A

E

E

D

D

L

P

L

L

F

F

V

I

L

L

Y

Y

T

T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

V

L

H

H

T

T

G

G

Y

Y

N

L

L

V

Y

Y

A

A

H

A

I

T

T

T

T

F

Q

K

W

Y

T

V

F

F

D

D

L

Y

Q

H

D

P

T

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

I

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

S

A

N

C

G

G

A

A

T

T

L

L

M

M

Y

F

E

E

G

G

A

V

P

P

R
x
N

A

W

S

P

N

T

P

P

G

A

C

R

F

M

W

C

D

Q

V

V

I

V

E

D

K

K

Y

H

G

Q

V

V

T

N

T

I

F

L

Y

Y

T

T

A
|
A

P

P

T

T

A

A

I

I

R

R

A

A

F

L

I

M

K

A

M

E

G

G

E

D

Q

K

H

A

P

I

A

E

A

G

R

T

D

D

L

R

S

S

L

L

R

R

L

I

L

L

G

G

T

S

V

V

G

G

E

E

P

P

I

I

N

N

P

P

E

E

A

A

W

W

I

E

W

W

Y

Y

H

W

R

K

V

K

I

I

G

G

G

K

D

E

R

K

C

C

P

P

I

V

V

V

D

D

T

T

W

W

Q

Q

T

T

E

E

T

T

G

G

H

F

M

M

I

I

T

T

S

P

L

L

P

P

G

G

A

A

V

I

P

E

T

L

K

K

P

A

G

G

S

S

A

A

T

T

K

R

P

P

F

F

P

F

G

G

I

V

L

Q

A

P

D

A

V

L

V

V

D

D

L

N

D

E

G

G

R

H

S

P

V

Q

P

E

D

G

N

A

E

T

G

E

G

G

Y

N

L

L

V

V

I

I

R

T

H

D

P

S

W

W

P

P

G

G

M

Q

M

A

R

R

T

T

V

L

Y

F

G

G

D

D

P

H

D

E

R

R

F

F

R

E

R

Q

T

T

Y

Y

W

F

E

S

H

T

I

F

P

-

Q

-

N

-

G

-

Q

K

Y

N

L

M

Y

Y

F

F

A

S

G

G

D

D

G

G

A

A

R

R

D

D

A

E

D

D

G

G

Y

Y

F

Y

W

W

V

I

M

T

G

G

R

R

V

V

D
|
D

D

D

V

V

I

L

N

N

V

V

S
|
S

G
|
G

H

H

R
|
R

L

L

G

G

T

T

M

A

E

E

I

I

E

E

S

S

A

A

L

L

V

V

S

A

H

H

P

P

A

K

V

I

A

A

E

E

A

A

V

V

G

G

R

I

P

P

D

H

D

A

L

I

K

K

G

G

E

Q

G

A

I

I

V

Y

A

A

F

Y

I

V

T

T

L

L

E

N

S

H

G

G

I

E

E

E

T

P

G

S

D

P

E

E

L

L

V

Y

K

A

D

E

L

V

K

R

K

N

H

W

V

V

A
x
R

Q

K

E

E

I

I

G

G

A

P

I

L

A

A

R

T

P

P

D

D

E

V

I

L

R

H

F

W

S

T

E

D

A

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

L

I

L

L

R

R

S

K

L

I

A

A

G

G

Q

D

E

T

V

S

S

N

-

L

G

G

D

D

T

T

S

S

T

T

L

L

E

A

D

D

R

P

S

G

V

V

L

V

D

E

K

K

L

L

R

L

Q

E

R194 (≠ A201) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T318) binding
N335 (≠ R342) binding
A357 (= A364) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D529) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (= S535) binding
G524 (= G536) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R538) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (≠ A596) binding ; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K621) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
54% identity, 97% coverage: 20:654/656 of query aligns to 7:636/640 of 5jrhA

query
sites
5jrhA

A

A

E

N

F

I

A

A

S

D

A

R

A

C

R

L

I

I

N

N

P

P

E

E

A

Q

Y

Y

E

E

A

T

L

K

C

Y

Q

K

K

Q

A

S

A

I

A

N

D

D

P

P

V

D

A

T

F

F

W

W

G

G

E

E

L

-

A

Q

A

G

Q

K

E

I

L

L

D

D

W

W

F

I

E

T

P

P

W

Y

Q

Q

Q

K

T

V

L

K

D

N

W

T

S

S

N

F

P

A

P

P

-

G

-

N

-

V

F

S

A

I

K

K

W

W

F

Y

V

E

G

D

G

G

K

T

L

L

N

N

I

L

S

A

H

A

N

N

C

C

L

L

D

D

R

R

H

H

L

L

T

Q

T

E

W

-

R

N

K

G

N

D

K

R

A

T

A

A

I

I

W
|
W

E

E

G

G

E

D

-

D

P

T

G

S

D

Q

S

S

R
x
K

T

H

L

I

T

S

Y

Y

A

R

Q

E

L

L

H

H

R

R

E

D

V

V

C

C

Q

R

F

F

A

A

N

N

V

T

L

L

K

L

S

D

L

L

G

G

I

I

Q

K

K

K

G

G

D

D

V

V

G

A

V

I

Y

Y

M

M

P

P

M

M

I
x
V

P

P

E
|
E

A

A

I

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

S

S

V

V

V

I

F
|
F

G
|
G

F

F

S

S

A

P

E

E

A

A

L

V

R

A

D
x
G

R
|
R

L

I

V

I

D

D

G

S

Q

S

A

S

K

R

L

L

V

V

V

I

T

T

A

A

D

D

G

E

G

G

W

V

R
|
R

K

A

D

G

A

R

I

S

V
x
I

P

P

L

L

K

K

D

K

S

N

V

V

D

D

Q

D

A

A

L

L

E

K

G

N

N

P

A

N

C

V

P

T

S

S

V

V

Q

E

H

H

V

V

L

I

V

V

V
x
L

E

K

R
|
R

T

T

K

G

Q

S

D

D

I

I

H

D

M

W

E

Q

P

E

G

G

R

R

D

D

H

L

W

W

H

R

E

D

L

L

Q

I

Q

E

T

K

V

A

S

S

A

P

T

E

C

H

P

Q

A

P

E

E

P

A

M

M

D

N

S

A

E

E

D

D

L

P

L

L

F

F

V

I

L

L

Y
|
Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K
|
K

P
|
P

K

K

G

G

V

V

V

L

H

H

T

T

G

G

Y

Y

N

L

L

V

Y

Y

A

A

H

A

I

T

T

T

T

F

Q

K

W

Y

T

V

F

F

D

D

L

Y

Q

H

D

P

T

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D
|
D

V

V

G

G

W

W

I

V

T

T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

S

A

N

C

G

G

A

A

T

T

L

L

M

M

Y

F

E

E

G

G

A

V

P

P

R

N

A

W

S

P

N

T

P

P

G

A

C

R

F

M

W

C

D

Q

V

V

I

V

E

D

K

K

Y

H

G

Q

V

V

T

N

T

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

A

A

F

L

I

M

K

A

M

E

G

G

E

D

Q

K

H

A

P

I

A

E

A

G

R

T

D

D

L

R

S

S

L

L

R

R

L

I

L

L

G

G

T

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

I

E

W

W

Y

Y

H

W

R

K

V

K

I

I

G

G

G

K

D

E

R

K

C

C

P

P

I

V

V

V

D
|
D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

H

F

M

M

I

I

T

T

S

P

L

L

P

P

G

G

A

A

V

I

P

E

T

L

K

K

P

A

G

G

S

S

A

A

T

T

K

R

P

P

F

F

P

F

G

G

I

V

L

Q

A

P

D

A

V

L

V

V

D

D

L

N

D

E

G

G

R

H

S

P

V

Q

P

E

D

G

N

A

E

T

G

E

G

G

Y

N

L

L

V

V

I

I

R

T

H

D

P

S

W

W

P

P

G

G

M

Q

M

A

R

R

T

T

V

L

Y

F

G

G

D

D

P

H

D

E

R

R

F

F

R

E

R

Q

T

T

Y

Y

W

F

E

S

H

T

I

F

P

-

Q

-

N

-

G

-

Q

K

Y

N

L

M

Y

Y

F

F

A

S

G

G

D
|
D

G

G

A

A

R

R

D

D

A

E

D

D

G

G

Y

Y

F

Y

W

W

V
x
I

M

T

G

G

R
|
R

V

V

D

D

V

V

I

L

N
|
N

V

V

S
|
S

G

G

H
|
H

R
|
R

L

L

G

G

T

T

M

A

E

E

I

I

E

E

S

S

A

A

L

L

V
|
V

S

A

H
|
H

P

P

A

K

V
x
I

A

A

E

E

A

A

V

V

G

G

R

I

P

P

D

H

D

A

L

I

K

K

G

G

E

Q

G

A

I

I

V

Y

A

A

F

Y

I

V

T

T

L

L

E

N

S

H

G

G

I

E

E

E

T

P

G

S

D

P

E

E

L

L

V

Y

K

A

D

E

L

V

K

R

K

N

H

W

V

V

A
x
R

Q

K

E

E

I

I

G

G

A
x
P

I

L

A

A

R

T

P

P

D

D

E

V

I

L

R

H

F

W

S

T

E

D

A

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

L

I

L

L

R

R

S

K

L

I

A

A

G

G

Q

D

E

-

V

-

S

L

G

G

D

D

T

T

S

S

T

T

L

L

E

A

D

D

R

P

S

G

V

V

L

V

D

E

K

K

L

L

R

L

Q

E

active site: T260 (= T271), T412 (= T423), E413 (= E424), N517 (= N533), R522 (= R538), K605 (= K621)
binding (r,r)-2,3-butanediol: W93 (= W105), K102 (≠ R113), V138 (≠ I149), E140 (= E151), G169 (≠ D180), R170 (= R181), L216 (≠ V227), R218 (= R229), Y259 (= Y270), K266 (= K277), P267 (= P278)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G394), E384 (= E395), P385 (= P396), D407 (= D418), T408 (= T419), W409 (= W420), W410 (= W421), Q411 (= Q422), T412 (= T423), D496 (= D512), I508 (≠ V524), R511 (= R527), N517 (= N533), R522 (= R538)
binding coenzyme a: F159 (= F170), G160 (= G171), G161 (= G172), R187 (= R198), I192 (≠ V203), D302 (= D313), S519 (= S535), H521 (= H537), R580 (≠ A596), P585 (≠ A601)
binding magnesium ion: V533 (= V549), H535 (= H551), I538 (≠ V554)

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
54% identity, 97% coverage: 20:654/656 of query aligns to 6:633/637 of 2p2fA

query
sites
2p2fA

A

A

E

N

F

I

A

A

S

D

A

R

A

C

R

L

I

I

N

N

P

P

E

E

A

Q

Y

Y

E

E

A

T

L

K

C

Y

Q

K

K

Q

A

S

A

I

A

N

D

D

P

P

V

D

A

T

F

F

W

W

G

G

E

E

L

-

A

Q

A

G

Q

K

E

I

L

L

D

D

W

W

F

I

E

T

P

P

W

Y

Q

Q

Q

K

T

V

L

K

D

N

W

T

S

S

N

F

P

A

P

P

-

G

-

N

-

V

F

S

A

I

K

K

W

W

F

Y

V

E

G

D

G

G

K

T

L

L

N

N

I

L

S

A

H

A

N

N

C

C

L

L

D

D

R

R

H

H

L

L

T

Q

T

E

W

-

R

N

K

G

N

D

K

R

A

T

A

A

I

I

W

W

E

E

G

G

E

D

-

D

P

T

G

S

D

Q

S

S

R

K

T

H

L

I

T

S

Y

Y

A

R

Q

E

L

L

H

H

R

R

E

D

V

V

C

C

Q

R

F

F

A

A

N

N

V

T

L

L

K

L

S

D

L

L

G

G

I

I

Q

K

K

K

G

G

D

D

V

V

G

A

V

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

A

A

I

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

S

S

V

V

V

I

F
|
F

G
|
G

F

F

S

S

A

P

E

E

A

A

L

V

R

A

D

G

R

R

L

I

V

I

D

D

G

S

Q

S

A

S

K

R

L

L

V

V

V

I

T

T

A

A

D

D

G

E

G

G

W

V

R
|
R

K

A

D

G

A

R

I

S

V
x
I

P

P

L

L

K

K

D

K

S

N

V

V

D

D

Q

D

A

A

L

L

E

K

G

N

N

P

A

N

C

V

P

T

S

S

V

V

Q

E

H

H

V

V

L

I

V

V

V

L

E

K

R

R

T

T

K

G

Q

S

D

D

I

I

H

D

M

W

E

Q

P

E

G

G

R

R

D

D

H

L

W

W

H

R

E

D

L

L

Q

I

Q

E

T

K

V

A

S

S

A

P

T

E

C

H

P

Q

A

P

E

E

P

A

M

M

D

N

S

A

E

E

D

D

L

P

L

L

F

F

V

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

V

L

H

H

T

T

G

G

Y

Y

N

L

L

V

Y

Y

A

A

H

A

I

T

T

T

T

F

Q

K

W

Y

T

V

F

F

D

D

L

Y

Q

H

D

P

T

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A
|
A

D

D

V

V

G

G

W
|
W

I

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

S

A

N

C

G

G

A

A

T

T

L

L

M

M

Y

F

E

E

G

G

A
x
V

P
|
P

R

N

A

W

S

P

N

T

P

P

G

A

C

R

F

M

W

C

D

Q

V

V

I

V

E

D

K

K

Y

H

G

Q

V

V

T

N

T

I

F

L

Y

Y

T

T

A
|
A

P

P

T
|
T

A
|
A

I

I

R

R

A

A

F

L

I

M

K

A

M

E

G

G

E

D

Q

K

H

A

P

I

A

E

A

G

R

T

D

D

L

R

S

S

L

L

R

R

L

I

L

L

G

G

T

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

I

E

W

W

Y

Y

H

W

R

K

V

K

I

I

G

G

G

K

D

E

R

K

C

C

P

P

I

V

V

V

D
|
D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

H

F

M

M

I

I

T

T

S

P

L

L

P

P

G

G

A

A

V

I

P

E

T

L

K

K

P

A

G

G

S

S

A

A

T

T

K

R

P

P

F

F

P

F

G

G

I

V

L

Q

A

P

D

A

V

L

V

V

D

D

L

N

D

E

G

G

R

H

S

P

V

Q

P

E

D

G

N

A

E

T

G

E

G

G

Y

N

L

L

V

V

I

I

R

T

H

D

P

S

W

W

P

P

G

G

M

Q

M

A

R

R

T

T

V

L

Y

F

G

G

D

D

P

H

D

E

R

R

F

F

R

E

R

Q

T

T

Y

Y

W

F

E

S

H

T

I

F

P

-

Q

-

N

-

G

-

Q

K

Y

N

L

M

Y

Y

F

F

A

S

G

G

D
|
D

G

G

A

A

R

R

D

D

A

E

D

D

G

G

Y

Y

F

Y

W

W

V
x
I

M

T

G

G

R
|
R

V

V

D

D

V

V

I

L

N
|
N

V

V

S
|
S

G
|
G

H

H

R
|
R

L

L

G

G

T

T

M

A

E

E

I

I

E

E

S

S

A

A

L

L

V

V

S

A

H

H

P

P

A

K

V

I

A

A

E

E

A

A

V

V

G

G

R

I

P

P

D

H

D

A

L

I

K

K

G

G

E

Q

G

A

I

I

V

Y

A

A

F

Y

I

V

T

T

L

L

E

N

S

H

G

G

I

E

E

E

T

P

G

S

D

P

E

E

L

L

V

Y

K

A

D

E

L

V

K

R

K

N

H

W

V

V

A
x
R

Q

K

E

E

I

I

G

G

A
x
P

I

L

A

A

R

T

P

P

D

D

E

V

I

L

R

H

F

W

S

T

E

D

A

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

L

I

L

L

R

R

S

K

L

I

A

A

G

G

Q

D

E

T

V

-

S

-

G

-

D

-

T

S

S

S

T

T

L

L

E

A

D

D

R

P

S

G

V

V

L

V

D

E

K

K

L

L

R

L

Q

E

active site: T259 (= T271), T411 (= T423), E412 (= E424), N516 (= N533), R521 (= R538), K604 (= K621)
binding adenosine monophosphate: G382 (= G394), E383 (= E395), P384 (= P396), D406 (= D418), T407 (= T419), W408 (= W420), W409 (= W421), Q410 (= Q422), T411 (= T423), D495 (= D512), I507 (≠ V524), R510 (= R527), N516 (= N533), R521 (= R538)
binding coenzyme a: F158 (= F170), G159 (= G171), G160 (= G172), R186 (= R198), I191 (≠ V203), A300 (= A312), W304 (= W316), T306 (= T318), V328 (≠ A340), P329 (= P341), A352 (= A364), T354 (= T366), A355 (= A367), S518 (= S535), G519 (= G536), R579 (≠ A596), P584 (≠ A601)

1pg3A Acetyl coa synthetase, acetylated on lys609 (see paper)
53% identity, 97% coverage: 20:654/656 of query aligns to 7:630/634 of 1pg3A

query
sites
1pg3A

A

A

E

N

F

I

A

A

S

D

A

R

A

C

R

L

I

I

N

N

P

P

E

E

A

Q

Y

Y

E

E

A

T

L

K

C

Y

Q

K

K

Q

A

S

A

I

A

N

D

D

P

P

V

D

A

T

F

F

W

W

G

G

E

E

L

-

A

Q

A

G

Q

K

E

I

L

L

D

D

W

W

F

I

E

T

P

P

W

Y

Q

Q

Q

K

T

V

L

K

D

N

W

T

S

S

N

F

P

A

P

P

-

G

-

N

-

V

F

S

A

I

K

K

W

W

F

Y

V

E

G

D

G

G

K

T

L

L

N

N

I

L

S

A

H

A

N

N

C

C

L

L

D

D

R

R

H

H

L

L

T

Q

T

E

W

-

R

N

K

G

N

D

K

R

A

T

A

A

I

I

W

W

E

E

G

G

E

D

-

D

P

T

G

S

D

Q

S

S

R

K

T

H

L

I

T

S

Y

Y

A

R

Q

E

L

L

H

H

R

R

E

D

V

V

C

C

Q

R

F

F

A

A

N

N

V

T

L

L

K

L

S

D

L

L

G

G

I

I

Q

K

K

K

G

G

D

D

V

V

G

A

V

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

A

A

I

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

S

S

V

V

V

I

F
|
F

G
|
G

F

F

S

S

A

P

E

E

A

A

L

V

R

A

D

G

R

C

L

I

V

I

D

D

G

S

Q

S

A

S

K

R

L

L

V

V

V

I

T

T

A

A

D

D

G

E

G

G

W

V

R
|
R

K

A

D

G

A
x
R

I

S

V
x
I

P

P

L

L

K

K

D

K

S

N

V

V

D

D

Q

D

A

A

L

L

E

K

G

N

N

P

A

N

C

V

P

T

S

S

V

V

Q

E

H

H

V

V

L

I

V

V

V

L

E

K

R

R

T

T

K

G

Q

S

D

D

I

I

H

D

M

W

E

Q

P

E

G

G

R

R

D

D

H

L

W

W

H

R

E

D

L

L

Q

I

Q

E

T

K

V

A

S

S

A

P

T

E

C

H

P

Q

A

P

E

E

P

A

M

M

D

N

S

A

E

E

D

D

L

P

L

L

F

F

V

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

V

L

H

H

T

T

G

G

Y

Y

N

L

L

V

Y

Y

A

A

H

A

I

T

T

T

T

F

Q

K

W

Y

T

V

F

F

D

D

L

Y

Q

H

D

P

T

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A
|
A

D

D

V

V

G

G

W

W

I
x
V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

S

A

N

C

G

G

A

A

T

T

L

L

M

M

Y

F

E

E

G

G

A
x
V

P
|
P

R
x
N

A

W

S

P

N

T

P

P

G

A

C

R

F

M

W

C

D

Q

V

V

I

V

E

D

K

K

Y

H

G

Q

V

V

T

N

T

I

F

L

Y

Y

T

T

A
|
A

P

P

T
|
T

A
|
A

I

I

R

R

A

A

F

L

I

M

K

A

M

E

G

G

E

D

Q

K

H

A

P

I

A

E

A

G

R

T

D

D

L

R

S

S

L

L

R

R

L

I

L

L

G

G

T

S

V
|
V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

I

E

W

W

Y

Y

H

W

R

K

V

K

I

I

G

G

G

K

D

E

R

K

C

C

P

P

I

V

V

V

D
|
D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

H

F

M

M

I

I

T

T

S

P

L

L

P

P

G

G

A

A

V

I

P

E

T

L

K

K

P

A

G

G

S

S

A

A

T

T

K

R

P

P

F

F

P

F

G

G

I

V

L

Q

A

P

D

A

V

L

V

V

D

D

L

N

D

E

G

G

R

H

S

P

V

Q

P

E

D

G

N

A

E

T

G

E

G

G

Y

N

L

L

V

V

I

I

R

T

H

D

P

S

W

W

P

P

G

G

M

Q

M

A

R

R

T

T

V

L

Y

F

G

G

D

D

P

H

D

E

R

R

F

F

R

E

R

Q

T

T

Y

Y

W

F

E

S

H

T

I

F

P

-

Q

-

N

-

G

-

Q

K

Y

N

L

M

Y

Y

F

F

A

S

G

G

D
|
D

G

G

A

A

R

R

D

D

A

E

D

D

G

G

Y

Y

F

Y

W

W

V
x
I

M

T

G

G

R
|
R

V

V

D

D

V

V

I

L

N
|
N

V

V

S
|
S

G
|
G

H
|
H

R
|
R

L

L

G

G

T

T

M

A

E

E

I

I

E

E

S

S

A

A

L

L

V
|
V

S

A

H
|
H

P

P

A

K

V
x
I

A

A

E

E

A

A

V

V

G

G

R

I

P

P

D

H

D

A

L

I

K

K

G

G

E

Q

G

A

I

I

V

Y

A

A

F

Y

I

V

T

T

L

L

E

N

S

H

G

G

I

E

E

E

T

P

G

S

D

P

E

E

L

L

V

Y

K

A

D

E

L

V

K

R

K

N

H

W

V

V

A
x
R

Q

K

E

E

I

I

G

G

A
x
P

I

L

A

A

R

T

P

P

D

D

E

V

I

L

R

H

F

W

S

T

E

D

A

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

L

I

L

L

R

R

S

K

L

I

A

A

G

G

Q

D

E

-

V

-

S

-

G

-

D

-

T

-

S

-

T

-

L

L

E

A

D

D

R

P

S

G

V

V

L

V

D

E

K

K

L

L

R

L

Q

E

active site: T260 (= T271), T412 (= T423), E413 (= E424), N517 (= N533), R522 (= R538), K605 (= K621)
binding coenzyme a: F159 (= F170), G160 (= G171), G161 (= G172), R187 (= R198), R190 (≠ A201), I192 (≠ V203),