SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Synpcc7942_1414 FitnessBrowser__SynE:Synpcc7942_1414 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
51% identity, 93% coverage: 8:230/241 of query aligns to 1:228/232 of 1f3oA

query
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binding adenosine-5'-diphosphate: Y11 (= Y18), G41 (= G48), G43 (= G50), K44 (= K51), S45 (= S52), T46 (= T53)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
51% identity, 93% coverage: 8:230/241 of query aligns to 1:228/230 of 1l2tA

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binding adenosine-5'-triphosphate: Y11 (= Y18), S40 (= S47), G41 (= G48), S42 (= S49), G43 (= G50), K44 (= K51), S45 (= S52), T46 (= T53), F138 (≠ L142), Q145 (= Q148), S147 (= S150), G149 (= G152), Q150 (= Q153), H204 (= H206)

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
49% identity, 93% coverage: 8:230/241 of query aligns to 3:225/615 of 5lilA

query
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5lilA

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binding adenosine-5'-triphosphate: F13 (≠ Y18), V22 (≠ A27), S42 (= S47), G43 (= G48), G45 (= G50), K46 (= K51), S47 (= S52), T48 (= T53), Q92 (= Q97), K136 (≠ R141), Q143 (= Q148), S145 (= S150), G147 (= G152), Q148 (= Q153)
binding magnesium ion: S47 (= S52), Q92 (= Q97)

5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
49% identity, 93% coverage: 8:230/241 of query aligns to 3:225/592 of 5lj7A

query
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5lj7A

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binding adenosine-5'-triphosphate: V22 (≠ A27), S42 (= S47), G43 (= G48), G45 (= G50), K46 (= K51), S47 (= S52), T48 (= T53), Q92 (= Q97), K136 (≠ R141), Q143 (= Q148), S145 (= S150), G147 (= G152), Q148 (= Q153)
binding magnesium ion: S47 (= S52), Q92 (= Q97)

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
50% identity, 92% coverage: 8:229/241 of query aligns to 3:224/226 of 5xu1B

query
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binding magnesium ion: G45 (= G50), K46 (= K51), S47 (= S52)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
51% identity, 94% coverage: 7:233/241 of query aligns to 3:226/648 of P75831

query
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P75831

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T

A

S

G

G

R

T

Y

Y

Y

R

L

V

D

A

G

G

T

Q

D

D

V

V

A

A

D

T

L

L

D

D

D

A

D

D

A

A

L

L

A

A

A

Q

V

L

R

R

N

R

R

E

K

H

I

F

G

G

F

F

V

I

F

F

Q

Q

Q

R

F

Y

H

H

L

L

L

L

P

S

Q

H

L

L

S

T

A

A

V

E

E

Q

N

N

V

V

M

E

L

V

P

P

M

A

I

V

Y

Y

A

A

G

G

I

L

S

E

Q

R

Q

K

E

Q

R

R

R

L

D

L

R

R

A

A

V

Q

A

E

A

L

L

L

T

Q

Q

R

V

L

G

G

L

L

A

E

Q

D

R

R

L

T

D

E

N

Y

K

Y

P

P

N

A

Q

Q

L

L

S

S

G

G

G

G

Q

Q

Q

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

I

L

V

M

N

N

Q

G

P

G

V

Q

L

V

L

I

L

L

A

A

D
|
D

E

E

P

P

T

T

G

G

A

A

L

L

D

D

S

S

Q

H

T

S

T

G

E

E

E

E

V

V

L

M

N

A

I

I

F

L

D

H

Q

Q

L

L

H

R

Q

D

R

R

G

G

I

H

T

T

I

V

V

I

I

I

V

V

T

T

H

H

E

D

A

P

E

Q

V

V

A

A

D

A

R

Q

A

A

E

E

R

R

V

V

I

I

W

E

F

I

R

R

D

D

G

G

Q

E

I

I

Q

V

R

R

E

-

T

-

Q

-

N

N

P

P

P

P

K47 (= K51) mutation to L: Lack of activity.
D169 (= D173) mutation to N: Lack of activity.

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
47% identity, 94% coverage: 8:233/241 of query aligns to 4:229/650 of 5ws4A

query
sites
5ws4A

V

L

I

L

Q

E

F

V

E

S

H

N

V

L

S

V

K

R

I

E

Y

F

G

P

E

A

G

G

E

E

T

S

T

T

V

I

R

Q

A

I

L

L

D

K

H

G

V

I

D

D

F

L

Q

T

V

I

R

Y

A

E

G

G

E

E

Y

L

C

V

A

A

I

I

M

V

G

G

A

Q

S
|
S

G
|
G

S

S

G

G

K

K

S
|
S

T

T

A

L

M

M

N

N

L

I

I

L

G

G

C

C

L

L

D

D

R

R

P

P

T

T

A

S

G

G

R

S

Y

Y

Y

K

L

V

D

N

G

G

T

Q

D

E

V

T

A

G

D

K

L

L

D

E

D

P

D

D

A

Q

L

L

A

A

A

Q

V

L

R

R

N

R

R

E

K

Y

I

F

G

G

F

F

V

I

F

F

Q

Q

Q

R

F

Y

H

H

L

L

L

L

P

G

Q

D

L

L

S

S

A

A

V

E

E

G

N

N

V

V

M

E

L

V

P

P

M

A

I

V

Y

Y

A

A

G

G

I

V

S

T

Q

P

Q

A

E

D

R

R

R

K

D

Q

R

R

A

A

V

T

A

A

A

L

L

L

T

T

Q

E

V

L

G

G

L

L

A

G

Q

T

R

K

L

T

D

Q

N

N

K

R

P

P

N

S

Q

Q

L

L

S

S

G

G

G

G

Q

Q

Q

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

I

L

V

M

N

N

Q

G

P

G

V

D

L

V

L

I

L

L

A

A

D

D

E

E

P

P

T

T

G

G

A

A

L

L

D

D

S

S

Q

H

T

S

T

G

E

V

E

E

V

V

L

M

N

R

I

I

F

L

D

R

Q

E

L

L

H

N

Q

A

R

A

G

G

I

H

T

T

I

I

V

I

I

L

V

V

T

T

H

H

E

D

A

M

E

Q

V

V

A

A

D

K

R

N

A

A

E

T

R

R

V

I

I

I

W

E

F

I

R

S

D

D

G

G

Q

E

I

I

Q

I

R

S

E

D

T

R

Q

P

N

N

P

V

P

P

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S43 (= S47), G44 (= G48), S48 (= S52)

2pclA Crystal structure of abc transporter with complex (aq_297) from aquifex aeolicus vf5
47% identity, 82% coverage: 28:225/241 of query aligns to 19:216/223 of 2pclA

query
sites
2pclA

L

L

D

K

H

G

V

I

D

S

F

L

Q

S

V

V

R

K

A

K

G

G

E

E

Y

F

C

V

A

S

I

I

M

I

G

G

A

A

S

S

G

G

S

S

G

G

K

K

S

S

T

T

A

L

M

L

N

Y

L

I

I

L

G

G

C

L

L

L

D

D

R

A

P

P

T

T

A

E

G

G

R

K

Y

V

Y

F

L

L

D

E

G

G

T

K

D

E

V

V

A

D

D

Y

L

T

D

N

D

E

D

K

A

E

L

L

A

S

A

L

V

L

R

R

N

N

R

R

K

K

I

L

G

G

F

F

V

V

F

F

Q

Q

Q

F

F

H

H

Y

L

L

L

I

P

P

Q

E

L

L

S

T

A

A

V

L

E

E

N

N

V

V

M

I

L

V

P

P

M

M

I

L

Y

K

A

M

G

G

I

K

S

P

Q

K

Q

K

E

E

R

A

R

K

D

E

R

R

A

G

V

E

A

Y

A

L

L

L

T

S

Q

E

V

L

G

G

L

L

A

G

Q

D

R

K

L

L

D

S

N

R

K

K

P

P

N

Y

Q

E

L

L

S

S

G

G

G

G

Q

E

Q

Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

N

N

Q

E

P

P

V

I

L

L

L

L

L

F

A

A

D

D

E
|
E

P
|
P

T
|
T

G

G

A

N

L

L

D

D

S

S

Q

A

T

N

T

T

E

K

E

R

V

V

L

M

N

D

I

I

F

F

D

L

Q

K

L

I

H

N

Q

E

R

G

G

G

I

T

T

S

I

I

V

V

I

M

V

V

T
|
T

H

H

E

E

A

R

E

E

V

L

A

A

D

E

R

L

A

T

E

H

R

R

V

T

I

L

W

E

F

M

R

K

D

D

G

G

Q

K

I

V

binding magnesium ion: E165 (= E174), P166 (= P175), T167 (= T176), T196 (= T205)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
42% identity, 92% coverage: 8:228/241 of query aligns to 5:226/233 of P75957

query
sites
P75957

V

L

I

L

Q

Q

F

C

E

D

H

N

V

L

S

C

K

K

I

R

Y

Y

G

Q

E

E

G

G

E

S

T

V

T

Q

V

T

R

D

A

V

L

L

D

H

H

N

V

V

D

S

F

F

Q

S

V

V

R

G

A

E

G

G

E

E

Y

M

C

M

A

A

I

I

M

V

G
|
G

A

S

S

S

G

G

S

S

G

G

K

K

S

S

T

T

A

L

M

L

N

H

L

L

I

L

G

G

C

G

L

L

D

D

R

T

P

P

T

T

A

S

G

G

R

D

Y

V

Y

I

L

F

D

N

G

G

T

Q

D

P

V

M

A

S

D

K

L

L

D

S

D

S

D

A

A

A

L

K

A

A

A

E

V

L

R

R

N

N

R

Q

K

K

I

L

G

G

F

F

V

I

F

Y

Q

Q

Q

F

F

H

H

H

L

L

L

L

P

P

Q

D

L

F

S

T

A

A

V

L

E

E

N

N

V

V

M

A

L

M

P

P

M

L

I

L

Y

I

A

G

G

K

I

K

S

K

Q

P

Q

A

E

E

R

I

R

N

D

S

R

R

A

A

V

L

A

E

A

M

L

L

T

K

Q

A

V

V

G

G

L

L

A

D

Q

H

R

R

L

A

D

N

N

H

K

R

P

P

N

S

Q

E

L

L

S

S

G

G

G

G

Q

E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

V

N

N

Q

N

P

P

V

R

L

L

L

V

L

L

A

A

D

D

E

E

P

P

T

T

G

G

A

N

L

L

D

D

S

A

Q

R

T

N

T

A

E

D

E

S

V

I

L

F

N

Q

I

L

F

L

D

G

Q

E

L

L

H

N

Q

R

-

L

R

Q

G

G

I

T

T

A

I

F

V

L

I

V

V

V

T

T

H

H

E

D

A

L

E

Q

V

L

A

A

D

K

R

R

A

M

E

S

R

R

V

Q

I

L

W

E

F

M

R

R

D

D

G

G

Q

R

I

L

Q

T

R

A

E

E

G42 (= G45) mutation to D: Loss of lipoprotein release when overexpressed.

7mdyC Lolcde nucleotide-bound
42% identity, 92% coverage: 8:228/241 of query aligns to 2:223/226 of 7mdyC

query
sites
7mdyC

V

L

I

L

Q

Q

F

C

E

D

H

N

V

L

S

C

K

K

I

R

Y
|
Y

G

Q

E

E

G

G

E

S

T

V

T

Q

V

T

R

D

A

V

L

L

D

H

H

N

V

V

D

S

F

F

Q

S

V

V

R

G

A

E

G

G

E

E

Y

M

C

M

A

A

I

I

M

V

G

G

A

S

S

S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

A

L

M

L

N

H

L

L

I

L

G

G

C

G

L

L

D

D

R

T

P

P

T

T

A

S

G

G

R

D

Y

V

Y

I

L

F

D

N

G

G

T

Q

D

P

V

M

A

S

D

K

L

L

D

S

D

S

D

A

A

A

L

K

A

A

A

E

V

L

R

R

N

N

R

Q

K

K

I

L

G

G

F

F

V

I

F

Y

Q
|
Q

Q

F

F

H

H

H

L

L

L

L

P

P

Q

D

L

F

S

T

A

A

V

L

E

E

N

N

V

V

M

A

L

M

P

P

M

L

I

L

Y

I

A

G

G

K

I

K

S

K

Q

P

Q

A

E

E

R

I

R

N

D

S

R

R

A

A

V

L

A

E

A

M

L

L

T

K

Q

A

V

V

G

G

L

L

A

D

Q

H

R

R

L

A

D

N

N
x
H

K

R

P

P

N

S

Q
x
E

L

L

S
|
S

G
|
G

G
|
G

Q

E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

V

N

N

Q

N

P

P

V

R

L

L

L

V

L

L

A

A

D

D

E
|
E

P

P

T

T

G

G

A
x
N

L

L

D

D

S

A

Q

R

T

N

T

A

E

D

E

S

V

I

L

F

N

Q

I

L

F

L

D

G

Q

E

L

L

H

N

Q

R

-

L

R

Q

G

G

I

T

T

A

I

F

V

L

I

V

V

V

T

T

H
|
H

E

D

A

L

E

Q

V

L

A

A

D

K

R

R

A

M

E

S

R

R

V

Q

I

L

W

E

F

M

R

R

D

D

G

G

Q

R

I

L

Q

T

R

A

E

E

binding adp orthovanadate: Y12 (= Y18), G42 (= G48), S43 (= S49), G44 (= G50), K45 (= K51), S46 (= S52), T47 (= T53), Q91 (= Q97), H138 (≠ N144), E142 (≠ Q148), S144 (= S150), G145 (= G151), G146 (= G152), E168 (= E174), N172 (≠ A178), H201 (= H206)
binding magnesium ion: S46 (= S52), Q91 (= Q97)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
42% identity, 90% coverage: 8:225/241 of query aligns to 2:220/222 of 7arlD

query
sites
7arlD

V

L

I

L

Q

Q

F

C

E

D

H

N

V

L

S

C

K

K

I

R

Y

Y

G

Q

E

E

G

G

E

S

T

V

T

Q

V

T

R

D

A

V

L

L

D

H

H

N

V

V

D

S

F

F

Q

S

V

V

R

G

A

E

G

G

E

E

Y

M

C

M

A

A

I

I

M

V

G

G

A

S

S

S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T

T

A

L

M

L

N

H

L

L

I

L

G

G

C

G

L

L

D

D

R

T

P

P

T

T

A

S

G

G

R

D

Y

V

Y

I

L

F

D

N

G

G

T

Q

D

P

V

M

A

S

D

K

L

L

D

S

D

S

D

A

A

A

L

K

A

A

A

E

V

L

R

R

N

N

R

Q

K

K

I

L

G

G

F

F

V

I

F

Y

Q

Q

Q

F

F

H

H

H

L

L

L

L

P

P

Q

D

L

F

S

T

A

A

V

L

E

E

N

N

V

V

M

A

L

M

P

P

M

L

I

L

Y

I

A

G

G

K

I

K

S

K

Q

P

Q

A

E

E

R

I

R

N

D

S

R

R

A

A

V

L

A

E

A

M

L

L

T

K

Q

A

V

V

G

G

L

L

A

D

Q

H

R

R

L

A

D

N

N

H

K

R

P

P

N

S

Q

E

L

L

S

S

G

G

G

G

Q

E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

V

N

N

Q

N

P

P

V

R

L

L

L

V

L

L

A

A

D

D

E

E

P

P

T

T

G

G

A

N

L

L

D

D

S

A

Q

R

T

N

T

A

E

D

E

S

V

I

L

F

N

Q

I

L

F

L

D

G

Q

E

L

L

H

N

Q

R

-

L

R

Q

G

G

I

T

T

A

I

F

V

L

I

V

V

V

T

T

H

H

E

D

A

L

E

Q

V

L

A

A

D

K

R

R

A

M

E

S

R

R

V

Q

I

L

W

E

F

M

R

R

D

D

G

G

Q

R

I

L

binding adenosine-5'-diphosphate: G42 (= G48), G44 (= G50), K45 (= K51), S46 (= S52)

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
41% identity, 92% coverage: 8:228/241 of query aligns to 4:225/229 of 7v8iD

query
sites
7v8iD

V

L

I

L

Q

Q

F

C

E

D

H

N

V

L

S

C

K

K

I

R

Y

Y

G

Q

E

E

G

G

E

S

T

V

T

Q

V

T

R

D

A
x
V

L

L

D

H

H

N

V

V

D

S

F

F

Q

S

V

V

R

G

A

E

G

G

E

E

Y

M

C

M

A

A

I

I

M

V

G

G

A

S

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

A

L

M

L

N

H

L

L

I

L

G

G

C

G

L

L

D

D

R

T

P

P

T

T

A

S

G

G

R

D

Y

V

Y

I

L

F

D

N

G

G

T

Q

D

P

V

M

A

S

D

K

L

L

D

S

D

S

D

A

A

A

L

K

A

A

A

E

V

L

R

R

N

N

R

Q

K

K

I

L

G

G

F

F

V

I

F

Y

Q
|
Q

Q

F

F

H

H

H

L

L

L

L

P

P

Q

D

L

F

S

T

A

A

V

L

E

E

N

N

V

V

M

A

L

M

P

P

M

L

I

L

Y

I

A

G

G

K

I

K

S

K

Q

P

Q

A

E

E

R

I

R

N

D

S

R

R

A

A

V

L

A

E

A

M

L

L

T

K

Q

A

V

V

G

G

L

L

A

D

Q

H

R
|
R

L

A

D

N

N
x
H

K

R

P

P

N

S

Q
x
E

L

L

S
|
S

G

G

G
|
G

Q
x
E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

V

N

N

Q

N

P

P

V

R

L

L

L

V

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

A

N

L

L

D

D

S

A

Q

R

T

N

T

A

E

D

E

S

V

I

L

F

N

Q

I

L

F

L

D

G

Q

E

L

L

H

N

Q

R

-

L

R

Q

G

G

I

T

T

A

I

F

V

L

I

V

V

V

T

T

H
|
H

E

D

A

L

E

Q

V

L

A

A

D

K

R

R

A

M

E

S

R

R

V

Q

I

L

W

E

F

M

R

R

D

D

G

G

Q

R

I

L

Q

T

R

A

E

E

binding phosphoaminophosphonic acid-adenylate ester: V23 (≠ A27), S43 (= S47), G44 (= G48), G46 (= G50), K47 (= K51), S48 (= S52), T49 (= T53), Q93 (= Q97), R137 (= R141), H140 (≠ N144), E144 (≠ Q148), S146 (= S150), G148 (= G152), E149 (≠ Q153), H203 (= H206)
binding magnesium ion: S48 (= S52), Q93 (= Q97)

P0A9R7 Cell division ATP-binding protein FtsE from Escherichia coli (strain K12) (see paper)
43% identity, 91% coverage: 8:226/241 of query aligns to 1:216/222 of P0A9R7

query
sites
P0A9R7

V

M

I

I

Q

R

F

F

E

E

H

H

V

V

S

S

K

K

I

A

Y

Y

G

L

E

G

G

G

E

R

T

-

T

-

V

-

R

Q

A

A

L

L

D

Q

H

G

V

V

D

T

F

F

Q

H

V

M

R

Q

A

P

G

G

E

E

Y

M

C

A

A

F

I

L

M

T

G

G

A

H

S

S

G

G

S

A

G

G

K
|
K

S

S

T

T

A

L

M

L

N

K

L

L

I

I

G
x
C

C

G

L

I

D

E

R

R

P

P

T

S

A

A

G

G

R

K

Y

I

Y

W

L

F

D

S

G

G

T

H

D

D

V

I

A

T

D

R

L

L

D

K

D

N

D

R

A

E

L

V

A

P

A

F

V

L

R

R

N

-

R

R

K

Q

I

I

G

G

F

M

V

I

F

F

Q

Q

Q

D

F

H

H

H

L

L

L

L

P

M

Q

D

L

R

S

T

A

V

V

Y

E

D

N

N

V

V

M

A

L

I

P

P

M

L

I

I

Y

I

A

A

G

G

I

A

S

S

Q

G

Q

D

E

D

R

I

R

R

D

R

R

R

A

V

V

S

A

A

A

A

L

L

T

D

Q

K

V

V

G

G

L

L

A

L

Q

D

R

K

L

A

D

K

N

N

K

F

P

P

N

I

Q

Q

L

L

S

S

G

G

G

G

Q

E

Q

Q

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

I

V

V

V

N

N

Q

K

P

P

V

A

L

V

L

L

L

L

A

A

D

D

E

E

P

P

T

T

G

G

A

N

L

L

D

D

S

D

Q

A

T

L

T

S

E

E

E

G

V

I

L

L

N

R

I

L

F

F

D

E

Q

E

L

F

H

N

Q

R

R

V

G

G

I

V

T

T

I

V

V

L

I

M

V

A

T

T

H

H

E

D

A

I

E

N

-

L

V

I

A

S

D

R

R

R

A

S

E

Y

R

R

V

M

I

L

W

T

F

L

R

S

D

D

G

G

Q

H

I

L

Q

H

K41 (= K51) mutation to R: Does not bind ATP.
C49 (≠ G59) mutation to A: Prevents dimer formation. Does not alter ATP-binding.

8w6iD Cryo-em structure of escherichia coli str k12 ftsex complex with atp- gamma-s in peptidisc
43% identity, 91% coverage: 8:226/241 of query aligns to 1:216/219 of 8w6iD

query
sites
8w6iD

V

M

I

I

Q

R

F

F

E

E

H

H

V

V

S

S

K

K

I

A

Y
|
Y

G

L

E

G

G

G

E

R

T

-

T

-

V

-

R

Q

A

A

L

L

D

Q

H

G

V

V

D

T

F

F

Q

H

V

M

R

Q

A

P

G

G

E

E

Y

M

C

A

A

F

I

L

M

T

G

G

A

H

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

A

L

M

L

N

K

L

L

I

I

G

C

C

G

L

I

D

E

R

R

P

P

T

S

A

A

G

G

R

K

Y

I

Y

W

L

F

D

S

G

G

T

H

D

D

V

I

A

T

D

R

L

L

D

K

D

N

D

R

A

E

L

V

A

P

A

F

V

L

R

R

N

-

R

R

K

Q

I

I

G

G

F

M

V

I

F

F

Q
|
Q

Q

D

F

H

H

H

L

L

L

L

P

M

Q

D

L

R

S

T

A

V

V

Y

E

D

N

N

V

V

M

A

L

I

P

P

M

L

I

I

Y

I

A

A

G

G

I

A

S

S

Q

G

Q

D

E

D

R

I

R

R

D

R

R

R

A

V

V

S

A

A

A

A

L

L

T

D

Q

K

V

V

G

G

L

L

A

L

Q

D

R
x
K

L

A

D

K

N

N

K

F

P

P

N

I

Q
|
Q

L

L

S
|
S

G
|
G

G
|
G

Q
x
E

Q

Q

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

I

V

V

V

N

N

Q

K

P

P

V

A

L

V

L

L

L

L

A

A

D

D

E

E

P

P

T

T

G

G

A

N

L

L

D

D

S

D

Q

A

T

L

T

S

E

E

E

G

V

I

L

L

N

R

I

L

F

F

D

E

Q

E

L

F

H

N

Q

R

R

V

G

G

I

V

T

T

I

V

V

L

I

M

V

A

T

T

H
|
H

E

D

A

I

E

N

-

L

V

I

A

S

D

R

R

R

A

S

E

Y

R

R

V

M

I

L

W

T

F

L

R

S

D

D

G

G

Q

H

I

L

Q

H

binding phosphothiophosphoric acid-adenylate ester: Y11 (= Y18), S37 (= S47), G38 (= G48), A39 (≠ S49), G40 (= G50), K41 (= K51), S42 (= S52), T43 (= T53), Q86 (= Q97), K130 (≠ R141), Q137 (= Q148), S139 (= S150), G140 (= G151), G141 (= G152), E142 (≠ Q153), H195 (= H206)

8hd0A Cell divisome spg hydrolysis machinery ftsex-envc
42% identity, 91% coverage: 8:226/241 of query aligns to 1:216/218 of 8hd0A

query
sites
8hd0A

V

M

I

I

Q

R

F

F

E

E

H

H

V

V

S

S

K

K

I

A

Y
|
Y

G

L

E

G

G

G

E
x
R

T

-

T

-

V

-

R

Q

A
|
A

L

L

D

Q

H

G

V

V

D

T

F

F

Q

H

V

M

R

Q

A

P

G

G

E

E

Y

M

C

A

A

F

I

L

M

T

G

G

A

H

S

S

G

G

S

A

G
|
G

K
|
K

S
|
S

T
|
T

A

L

M

L

N

K

L

L

I

I

G

C

C

G

L

I

D

E

R

R

P

P

T

S

A

A

G

G

R

K

Y

I

Y

W

L

F

D

S

G

G

T

H

D

D

V

I

A

T

D

R

L

L

D

K

D

N

D

R

A

E

L

V

A

P

A

F

V

L

R

R

N

-

R

R

K

Q

I

I

G

G

F

M

V

I

F

F

Q

Q

Q

D

F

H

H

H

L

L

L

L

P

M

Q

D

L

R

S

T

A

V

V

Y

E

D

N

N

V

V

M

A

L

I

P

P

M

L

I

I

Y

I

A

A

G

G

I

A

S

S

Q

G

Q

D

E

D

R

I

R

R

D

R

R

R

A

V

V

S

A

A

A

A

L

L

T

D

Q

K

V

V

G

G

L

L

A

L

Q

D

R

K

L

A

D

K

N

N

K

F

P

P

N

I

Q

Q

L

L

S
|
S

G

G

G
|
G

Q

E

Q

Q

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

I

V

V

V

N

N

Q

K

P

P

V

A

L

V

L

L

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

A

N

L

L

D

D

S

D

Q

A

T

L

T

S

E

E

E

G

V

I

L

L

N

R

I

L

F

F

D

E

Q

E

L

F

H

N

Q

R

R

V

G

G

I

V

T

T

I

V

V

L

I

M

V

A

T

T

H

H

E

D

A

I

E

N

-

L

V

I

A

S

D

R

R

R

A

S

E

Y

R

R

V

M

I

L

W

T

F

L

R

S

D

D

G

G

Q

H

I

L

Q

H

binding adenosine-5'-triphosphate: Y11 (= Y18), R15 (≠ E22), A17 (= A27), G40 (= G50), K41 (= K51), S42 (= S52), T43 (= T53), S139 (= S150), G141 (= G152)

8g4cB Bceabs atpgs high res tm (see paper)
36% identity, 90% coverage: 8:225/241 of query aligns to 3:221/248 of 8g4cB

query
sites
8g4cB

V

I

I

L

Q

E

F

A

E

N

H

K

V

I

S

R

K

K

I

S

Y
|
Y

G

G

E

N

G

K

E

L

T

N

T

K

V

Q

R

E

A

V

L

L

D

K

H

G

V

I

D

D

F

I

Q

H

V

I

R

E

A

K

G

G

E

E

Y

F

C

V

A

S

I

I

M

M

G

G

A

A

S
|
S

G

G

S
|
S

G

G

K

K

S
x
T

T

T

A

L

M

L

N

N

L

V

I

L

G

S

C

S

L

I

D

D

R

Q

P

V

T

S

A

H

G

G

R

T

Y

I

Y

H

L

I

D

N

G

G

T

N

D

D

V

M

A

T

D

A

L

M

D

K

D

E

D

K

A

Q

L

L

A

A

A

E

V

F

R

R

N

K

R

Q

K

H

I

L

G

G

F

F

V

I

F

F

Q

Q

Q

D

F

Y

H

N

L

L

L

L

P

D

Q

T

L

L

S

T

A

V

V

K

E

E

N

N

V

I

M

L

L

L

P

P

M

L

I

S

Y

I

A

T

G

K

I

L

S

S

Q

K

Q

K

E

E

R

A

R

N

D

R

R

K

A

F

V

E

A

E

A

V

L

A

T

K

Q

E

V

L

G

G

L

I

A

Y

Q

E

R

L

L

R

D

D

N

K

K

Y

P

P

N

N

Q

E

L

I

S

S

G

G

G

G

Q

Q

Q

K

Q

Q

R

R

V

T

A

S

I

A

A

G

R

R

A

A

I

F

V

I

N

H

Q

D

P

P

V

S

L

I

L

I

L

F

A

A

D
|
D

E

E

P

P

T

T

G

G

A

A

L

L

D

D

S

S

Q

K

T

S

T

A

E

S

E

D

V

L

L

L

N

N

I

K

F

L

D

S

Q

Q

L

L

H

N

Q

Q

-

K

R

R

G

N

I

A

T

T

I

I

V

I

I

M

V

V

T

T

H

H

E

D

A

P

E

V

V

A

A

A

D

S

R

Y

A

C

E

G

R

R

V

V

I

I

W

F

F

I

R

K

D

D

G

G

Q

Q

I

M

binding phosphothiophosphoric acid-adenylate ester: Y13 (= Y18), S42 (= S47), S44 (= S49), T47 (≠ S52), D168 (= D173)

7tchB Bceab e169q variant atp-bound conformation (see paper)
36% identity, 90% coverage: 8:225/241 of query aligns to 2:220/245 of 7tchB

query
sites
7tchB

V

I

I

L

Q

E

F

A

E

N

H

K

V

I

S

R

K

K

I

S

Y
|
Y

G

G

E

N

G

K

E

L

T

N

T

K

V
x
Q

R

E

A
x
V

L

L

D

K

H

G

V

I

D

D

F

I

Q

H

V

I

R

E

A

K

G

G

E

E

Y

F

C

V

A

S

I

I

M

M

G

G

A

A

S
|
S

G
|
G

S
|
S

G

G

K
|
K

S
x
T

T

T

A

L

M

L

N

N

L

V

I

L

G

S

C

S

L

I

D

D

R

Q

P

V

T

S

A

H

G

G

R

T

Y

I

Y

H

L

I

D

N

G

G

T

N

D

D

V

M

A

T

D

A

L

M

D

K

D

E

D

K

A

Q

L

L

A

A

A

E

V

F

R

R

N

K

R

Q

K

H

I

L

G

G

F

F

V

I

F

F

Q

Q

Q

D

F

Y

H

N

L

L

L

L

P

D

Q

T

L

L

S

T

A

V

V

K

E

E

N

N

V

I

M

L

L

L

P

P

M

L

I

S

Y

I

A

T

G

K

I

L

S

S

Q

K

Q

K

E

E

R

A

R

N

D

R

R

K

A

F

V

E

A

E

A

V

L

A

T

K

Q

E

V

L

G

G

L

I

A

Y

Q

E

R

L

L

R

D

D

N

K

K

Y

P

P

N

N

Q
x
E

L

I

S

S

G
|
G

G
|
G

Q

Q

Q

K

Q

Q

R

R

V

T

A

S

I

A

A

G

R

R

A

A

I

F

V

I

N

H

Q

D

P

P

V

S

L

I

L

I

L

F

A

A

D

D

E

Q

P

P

T

T

G

G

A

A

L

L

D

D

S

S

Q

K

T

S

T

A

E

S

E

D

V

L

L

L

N

N

I

K

F

L

D

S

Q

Q

L

L

H

N

Q

Q

-

K

R

R

G

N

I

A

T

T

I

I

V

I

I

M

V

V

T

T

H

H

E

D

A

P

E

V

V

A

A

A

D

S

R

Y

A

C

E

G

R

R

V

V

I

I

W

F

F

I

R

K

D

D

G

G

Q

Q

I

M

binding adenosine-5'-triphosphate: Y12 (= Y18), Q19 (≠ V25), V21 (≠ A27), S41 (= S47), G42 (= G48), S43 (= S49), K45 (= K51), T46 (≠ S52), E142 (≠ Q148), G145 (= G151), G146 (= G152)

8tzjA Cryo-em structure of vibrio cholerae ftse/ftsx complex (see paper)
44% identity, 85% coverage: 8:212/241 of query aligns to 2:202/220 of 8tzjA

query
sites
8tzjA

V

V

I

I

Q

R

F

F

E

Q

H

Q

V

V

S

S

K

K

I

A

Y
|
Y

G

R

E

G

G

G

E
x
R

T

-

T

-

V

-

R

Q

A
|
A

L

L

D

Q

H

K

V

V

D

D

F

F

Q

H

V

L

R

R

A

R

G

G

E

E

Y

M

C

A

A

F

I

L

M

G

G

G

A

H

S

S

G
|
G

S

A

G
|
G

K
|
K

S
|
S

T

T

A

L

M

L

N

K

L

L

I

I

G

C

C

A

L

I

D

E

R

R

P

P

T

T

A

D

G

G

R

K

Y

I

Y

S

L

F

D

N

G

G

T

H

D

D

V

I

A

T

D

R

L

I

D

P

D

N

D

K

A

D

L

I

A

P

A

F

V

L

R

R

N

-

R

R

K

N

I

I

G

G

F

I

V

V

F

F

Q

Q

Q

D

F

H

H

R

L

L

L

L

P

M

Q

D

L

R

S

S

A

I

V

Y

E

D

N

N

V

V

M

A

L

L

P

P

M

M

I

R

Y

I

A

E

G

S

I

I

S

S

Q

E

Q

N

E

E

R

I

R

K

D

R

R

R

A

V

V

S

A

A

A

A

L

L

T

D

Q

K

V

T

G

G

L

L

A

L

Q

D

R

K

L

A

D

R

N

C

K

L

P

P

N

S

Q

Q

L

L

S

S

G

G

G

G

Q

E

Q

Q

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

I

V

V

V

N

N

Q

R

P

P

V

T

L

L

L

L

L

L

A

A

D

D

E

E

P

P

T

T

G

G

A

N

L

L

D

D

S

P

Q

E

T

L

T

S

E

S

E

R

V

V

L

L

N

R

I

L

F

F

D

E

Q

E

L

F

H

N

Q

R

R

A

G

G

I

V

T

T

I

I

V

L

I

L

V

A

T

T

H

H

E

D

A

I

E

H

V

L

A

V

D

N

binding adenosine-5'-diphosphate: Y12 (= Y18), R16 (≠ E22), A18 (= A27), G39 (= G48), G41 (= G50), K42 (= K51), S43 (= S52)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
43% identity, 85% coverage: 24:228/241 of query aligns to 13:217/240 of 4ymuJ

query
sites
4ymuJ

T

S

V

L

R

E

A
x
V

L

L

D

K

H

G

V

V

D

T

F

L

Q

K

V

V

R

N

A

K

G

G

E

E

Y

V

C

V

A

V

I

I

M

I

G

G

A

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

A

L

M

L

N

R

L

C

I

I

G

N

C

L

L

L

D

E

R

E

P

P

T

T

A

K

G

G

R

E

Y

V

Y

F

L

I

D

D

G

G

T

V

D

K

V

I

A

N

D

N

L

-

D

G

D

K

D

V

A

N

L

I

A

N

A

K

V

V

R

R

N

-

R

Q

K

K

I

V

G

G

F

M

V

V

F

F

Q

Q

Q

H

F

F

H

N

L

L

L

F

P

P

Q

H

L

L

S

T

A

A

V

I

E

E

N

N

V

I

M

T

L

L

-

A

P

P

M

V

I

K

Y

V

A

K

G

K

I

M

S

N

Q

K

Q

K

E

E

R

A

R

E

D

E

R

L

A

A

V

V

A

D

A

L

L

L

T

A

Q

K

V

V

G

G

L

L

A

L

Q

D

R

K

L

K

D

D

N

Q

K

Y

P

P

N

I

Q

K

L

L

S

S

G

G

G

G

Q

Q

Q

K

Q

Q

R

R

V

L

A

A

I

I

A

A

R

R

A

A

I

L

V

A

N

M

Q

Q

P

P

V

E

L

V

L

M

L

L

A

F

D

D

E
|
E

P

P

T

T

G

S

A

A

L

L

D

D

S

P

Q

E

T

M

T

V

E

K

E

E

V

V

L

L

N

N

I

V

F

M

D

K

Q

Q

L

L

H

A

Q

N

R

E

G

G

I

M

T

T

I

M

V

V

I

V

V

V

T

T

H
|
H

E

E

A

M

E

G

V

F

A

A

-

R

D

E

R

V

A

G

E

D

R

R

V

V

I

I

W

F

F

M

R

D

D

D

G

G

Q

V

I

I

Q

V

R

E

E

E

binding adenosine-5'-triphosphate: V16 (≠ A27), S36 (= S47), G37 (= G48), S38 (= S49), G39 (= G50), K40 (= K51), S41 (= S52), T42 (= T53), E162 (= E174), H194 (= H206)
binding magnesium ion: S41 (= S52), E162 (= E174)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 11

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
40% identity, 85% coverage: 8:213/241 of query aligns to 1:206/343 of P30750

query
sites
P30750

V

M

I

I

Q

K

F

L

E

S

H

N

V

I

S

T

K

K

I

V

Y

F

G

H

E

Q

G

G

E

T

T

R

T

T

V

I

R

Q

A

A

L

L

D

N

H

N

V

V

D

S

F

L

Q

H

V

V

R

P

A

A

G

G

E

Q

Y

I

C

Y

A

G

I

V

M

I

G

G

A

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

A

L

M

I

N

R

L

C

I

V

G

N

C

L

L

L

D

E

R

R

P

P

T

T

A

E

G

G

R

S

Y

V

Y

L

L

V

D

D

G

G

T

Q

D

E

V

L

A

T

D

T

L

L

D

S

D

E

D

S

A

E

L

L

A

T

A

K

V

A

R

R

N

-

R

R

K

Q

I

I

G

G

F

M

V

I

F

F

Q

Q

Q

H

F

F

H

N

L

L

L

L

P

S

Q

S

L

R

S

T

A

V

V

F

E

G

N

N

V

V

M

A

L

L

P

P

M

L

I

E

Y

L

A

D

G

N

I

T

S

P

Q

K

Q

D

E

E

R

V

R

K

D

R

R

R

A

V

V

T

A

E

A

L

L

L

T

S

Q

L

V

V

G

G

L

L

A

G

Q

D

R

K

L

H

D

D

N

S

K

Y

P

P

N

S

Q

N

L

L

S

S

G

G

G

G

Q

Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

N

S

Q

N

P

P

V

K

L

V

L

L

L

L

A

C

D

D

E
|
E

P

A

T

T

G

S

A

A

L

L

D

D

S

P

Q

A

T

T

T

T

E

R

E

S

V

I

L

L

N

E

I

L

F

L

D

K

Q

D

L

I

H

N

Q

R

R

R

-

L

G

G

I

L

T

T

I

I

V

L

I

L

V

I

T

T

H

H

E

E

A

M

E

D

V

V

A

V

D

K

R

R

40:46 (vs. 47:53, 86% identical) binding
E166 (= E174) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

Query Sequence

>Synpcc7942_1414 FitnessBrowser__SynE:Synpcc7942_1414
MAESAPPVIQFEHVSKIYGEGETTVRALDHVDFQVRAGEYCAIMGASGSGKSTAMNLIGC
LDRPTAGRYYLDGTDVADLDDDALAAVRNRKIGFVFQQFHLLPQLSAVENVMLPMIYAGI
SQQERRDRAVAALTQVGLAQRLDNKPNQLSGGQQQRVAIARAIVNQPVLLLADEPTGALD
SQTTEEVLNIFDQLHQRGITIVIVTHEAEVADRAERVIWFRDGQIQRETQNPPRPVLAAT
F

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory