SitesBLAST

P61459 Pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha; DCoH; Dimerization cofactor of HNF1; Phenylalanine hydroxylase-stimulating protein; Pterin carbinolamine dehydratase; PCD; EC 4.2.1.96 from Rattus norvegicus (Rat) (see 2 papers)
37% identity, 65% coverage: 48:135/135 of query aligns to 8:99/104 of P61459

query
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P61459

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C82 (≠ A118) mutation to R: Reduced dehydratase activity. No impact on hydroxytetrahydrobiopterin-binding.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

P61457 Pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha; DCoH; Dimerization cofactor of HNF1; Phenylalanine hydroxylase-stimulating protein; Pterin carbinolamine dehydratase; PCD; EC 4.2.1.96 from Homo sapiens (Human) (see 6 papers)
37% identity, 65% coverage: 48:135/135 of query aligns to 8:99/104 of P61457

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P61457

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T79 (= T115) to I: in HPABH4D; increased proteolytic degradation; reduced interaction with HNF1B; loss of HNF1B-coactivator activity; dbSNP:rs121913014
C82 (≠ A118) to R: in HPABH4D; increased proteolytic degradation; reduced dehydratase activity; no impact on hydroxytetrahydrobiopterin-binding; reduced interaction with HNF1B; partial impact on HNF1B-coactivator activity; dbSNP:rs104894177
R88 (≠ Q124) to Q: in HPABH4D; when associated in cis with 27-E--T-104 del; uncertain significance; no impact on HNF1B interaction; no impact on HNF1B-coactivator activity; dbSNP:rs115117837
E97 (≠ S133) to K: in HPABH4D; increased proteolytic degradation; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs397518416

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
27:104 natural variant: Missing (in HPABH4D; when associated in cis with Q-88; reduced protein levels; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs727505360)
87:104 natural variant: Missing (in HPABH4D; increased proteolytic degradation; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs104894172)
98:104 natural variant: Missing (in HPABH4D; increased proteolytic degradation; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs121913015)

1dcpA Dcoh, a bifunctional protein-binding transcriptional coactivator, complexed with biopterin (see paper)
37% identity, 65% coverage: 48:135/135 of query aligns to 3:94/99 of 1dcpA

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1dcpA

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active site: E53 (= E94), H57 (= H98), H58 (= H99), H75 (= H116), E76 (≠ D117), D84 (= D125)
binding 7,8-dihydrobiopterin: D56 (≠ G97), H57 (= H98), H58 (= H99), T74 (= T115), H75 (= H116)

1f93A Crystal structure of a complex between the dimerization domain of hnf-1 alpha and the coactivator dcoh (see paper)
36% identity, 58% coverage: 58:135/135 of query aligns to 19:98/103 of 1f93A

query
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1f93A

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active site: E57 (= E94), H61 (= H98), H62 (= H99), H79 (= H116), E80 (≠ D117), D88 (= D125)
binding : N43 (≠ P80), R44 (≠ E81), G47 (≠ A84), R51 (= R88), L54 (≠ E91)

Q02I31 Putative pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Pterin carbinolamine dehydratase; PCD; EC 4.2.1.96 from Pseudomonas aeruginosa (strain UCBPP-PA14) (see paper)
28% identity, 65% coverage: 48:135/135 of query aligns to 19:110/118 of Q02I31

query
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Q02I31

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: Phosphothreonine

Query Sequence

>Synpcc7942_1636 FitnessBrowser__SynE:Synpcc7942_1636
MPRLRWTSYLKVLVISLIAGTLSPVVASLPSQGTRPSLARSMEMNSVLSAAEIQTRLLRL
PAWRLDGQSIVCDRRFAGFPEAIAFLNRLVEPAEAAGHHPDLEVSYNRVIIRLTTHDAGG
LTDQDFSLAEKISQL

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory