SitesBLAST
Comparing Synpcc7942_2557 FitnessBrowser__SynE:Synpcc7942_2557 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7t30A Structure of electron bifurcating ni-fe hydrogenase complex hydabcsl in fmn/NAD(h) bound state (see paper)
43% identity, 87% coverage: 1:207/238 of query aligns to 1:207/666 of 7t30A
- binding fe2/s2 (inorganic) cluster: C36 (= C36), G45 (= G45), C47 (= C47), R48 (= R48), C50 (= C50), C64 (= C64)
- binding iron/sulfur cluster: F93 (= F93), H98 (= H98), F99 (≠ V99), C100 (= C100), C103 (= C103), Q105 (≠ A105), C109 (= C109), L141 (≠ F141), R147 (= R147), C148 (= C148), L150 (= L150), C151 (= C151), Q152 (≠ T152), C154 (= C154), C158 (= C158), C192 (= C192), V193 (≠ T193), N194 (= N194), C195 (= C195), G196 (= G196), A197 (≠ K197), C198 (= C198), C202 (= C202), P203 (= P203), T204 (= T204), G205 (= G205), T206 (≠ A206), I207 (≠ L207)
Sites not aligning to the query:
6tg9A Cryo-em structure of nadh reduced form of NAD+-dependent formate dehydrogenase from rhodobacter capsulatus (see paper)
39% identity, 91% coverage: 4:219/238 of query aligns to 19:246/949 of 6tg9A
- binding fe2/s2 (inorganic) cluster: C51 (= C36), V59 (= V44), G60 (= G45), C62 (= C47), C65 (= C50), C79 (= C64)
- binding iron/sulfur cluster: H111 (≠ G96), C115 (= C100), C118 (= C103), A120 (= A105), C124 (= C109), C176 (= C148), I177 (= I149), V178 (≠ L150), C179 (= C151), M180 (≠ T152), C182 (= C154), C186 (= C158), I206 (= I178), C218 (= C192), S220 (≠ N194), C221 (= C195), G222 (= G196), C224 (= C198), C228 (= C202), T230 (= T204), A231 (≠ G205)
Sites not aligning to the query:
- active site: 289, 380, 381, 545, 582, 583
- binding 2-amino-5,6-dimercapto-7-methyl-3,7,8a,9-tetrahydro-8-oxa-1,3,9,10-tetraaza-anthracen-4-one guanosine dinucleotide: 255, 289, 351, 352, 380, 414, 415, 419, 420, 421, 443, 444, 464, 467, 545, 546, 550, 582, 583, 583, 649, 650, 655, 680, 693, 698, 724, 820, 821, 823, 823, 824, 825, 829, 830, 833, 902, 918, 919
- binding iron/sulfur cluster: 252, 254, 255, 257, 259, 261, 287, 289, 423
8a5eA Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from acetobacterium woodii in the reduced state (see paper)
36% identity, 95% coverage: 1:225/238 of query aligns to 1:220/583 of 8a5eA
- binding fe2/s2 (inorganic) cluster: T34 (= T34), C36 (= C36), G45 (= G45), C47 (= C47), C50 (= C50), C64 (= C64)
- binding iron/sulfur cluster: H96 (≠ G96), N97 (= N97), C100 (= C100), C103 (= C103), S106 (≠ N106), C109 (= C109), C148 (= C148), C151 (= C151), K152 (≠ T152), C154 (= C154), C158 (= C158), V166 (= V166), C191 (= C192), C194 (= C195), G195 (= G196), C197 (= C198), C201 (= C202), P202 (= P203), V203 (≠ T204)
Sites not aligning to the query:
8a6tA Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from thermoanaerobacter kivui in the reduced state (see paper)
37% identity, 88% coverage: 1:210/238 of query aligns to 1:207/571 of 8a6tA
- binding fe2/s2 (inorganic) cluster: C36 (= C36), D37 (≠ H37), C47 (= C47), R48 (= R48), C50 (= C50), C63 (= C64)
- binding iron/sulfur cluster: H95 (≠ G96), C99 (= C100), C102 (= C103), C108 (= C109), C146 (= C148), C149 (= C151), G150 (≠ T152), K151 (≠ R153), C152 (= C154), C156 (= C158), C189 (= C192), C192 (= C195), C195 (= C198), C199 (= C202), G202 (= G205)
Sites not aligning to the query:
- binding 2 iron/2 sulfur/5 carbonyl/2 water inorganic cluster: 229, 230, 231, 298, 323, 352, 353, 357, 412, 500
- binding iron/sulfur cluster: 299, 354, 496, 500
5xf9B Crystal structure of NAD+-reducing [nife]-hydrogenase in the air- oxidized state (see paper)
39% identity, 87% coverage: 5:210/238 of query aligns to 4:206/234 of 5xf9B
- binding fe2/s2 (inorganic) cluster: C35 (= C36), G44 (= G45), C46 (= C47), R47 (= R48), C49 (= C50), C61 (= C64)
- binding iron/sulfur cluster: F90 (= F93), H95 (= H98), F96 (≠ V99), C97 (= C100), C100 (= C103), S103 (≠ N106), C106 (= C109), C145 (= C148), I146 (= I149), L147 (= L150), C148 (= C151), G149 (≠ T152), C151 (= C154), L174 (≠ I178), C198 (= C202), G201 (= G205), A202 (= A206)
7p8na Tmhydabc- t. Maritima hydrogenase with bridge closed (see paper)
36% identity, 93% coverage: 4:224/238 of query aligns to 1:210/642 of 7p8na
- binding fe2/s2 (inorganic) cluster: N32 (≠ T34), C34 (= C36), Y42 (≠ V44), G43 (= G45), C45 (= C47), R46 (= R48), C48 (= C50), C60 (= C64)
- binding iron/sulfur cluster: H92 (≠ G96), D95 (≠ V99), C96 (= C100), C99 (= C103), C105 (= C109), Q108 (= Q112), C143 (= C148), I144 (= I149), L145 (= L150), C146 (= C151), G147 (≠ T152), D148 (≠ R153), C149 (= C154), C153 (= C158), V161 (= V166), C186 (= C192), V187 (≠ T193), L188 (≠ N194), C189 (= C195), G190 (= G196), C192 (= C198), C196 (= C202), P197 (= P203), T198 (= T204), A200 (= A206), L201 (= L207)
Sites not aligning to the query:
- binding fe2/s2 (inorganic) cluster: 575, 577, 579, 580, 612, 613, 615, 616
- binding iron/sulfur cluster: 295, 350, 480, 481, 482, 486
7zm7A Cryoem structure of mitochondrial complex i from chaetomium thermophilum (inhibited by ddm) (see paper)
33% identity, 87% coverage: 4:210/238 of query aligns to 3:207/711 of 7zm7A
- binding fe2/s2 (inorganic) cluster: R33 (≠ T34), C35 (= C36), Y36 (≠ H37), G44 (= G45), C46 (= C47), R47 (= R48), C49 (= C50), C63 (= C64)
- binding iron/sulfur cluster: H95 (≠ G96), D98 (≠ V99), C99 (= C100), C102 (= C103), Q104 (≠ A105), G105 (≠ N106), C108 (= C109), Q111 (= Q112), C149 (= C148), I150 (= I149), C152 (= C151), T153 (= T152), C155 (= C154), I179 (= I178), C199 (= C202), V201 (≠ T204), L204 (= L207)
7q5yA Structure of nadh:ubichinon oxidoreductase (complex i) of the hyperthermophilic eubacterium aquifex aeolicus
34% identity, 87% coverage: 4:210/238 of query aligns to 2:211/626 of 7q5yA
- binding fe2/s2 (inorganic) cluster: C34 (= C36), Y35 (≠ H37), G43 (= G45), C45 (= C47), R46 (= R48), C48 (= C50), C62 (= C64)
- binding iron/sulfur cluster: H96 (≠ G96), D99 (≠ V99), C100 (= C100), C103 (= C103), A106 (≠ N106), C109 (= C109), Q112 (= Q112), C151 (= C148), V152 (≠ I149), V153 (≠ L150), C154 (= C151), Y155 (≠ T152), C157 (= C154), C161 (= C158), T167 (≠ A164), C193 (= C192), M195 (≠ N194), C196 (= C195), G197 (= G196), C199 (= C198), C203 (= C202), P204 (= P203), A207 (= A206), I208 (≠ L207)
Sites not aligning to the query:
P29915 NADH-quinone oxidoreductase chain 3; NADH dehydrogenase I, chain 3; NDH-1, chain 3; EC 7.1.1.- from Paracoccus denitrificans (see 2 papers)
35% identity, 86% coverage: 6:210/238 of query aligns to 7:216/673 of P29915
- H106 (≠ G96) mutation to A: Very little incorporation of iron-sulfur centers into protein in E.coli.; mutation to C: Alters the EPR signal of the N5 cluster; all 3 iron-sulfur clusters are less stable in E.coli.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
7b0nG 3.7-angstrom structure of Yarrowia lipolytica complex I with an R121M mutation in NUCM. (see paper)
30% identity, 87% coverage: 4:210/238 of query aligns to 3:207/694 of 7b0nG
- binding fe2/s2 (inorganic) cluster: C35 (= C36), G44 (= G45), C46 (= C47), R47 (= R48), C49 (= C50), C63 (= C64)
- binding iron/sulfur cluster: H95 (≠ G96), C99 (= C100), C102 (= C103), C108 (= C109), Q111 (= Q112), C149 (= C148), H151 (≠ L150), C152 (= C151), T153 (= T152), C155 (= C154), C199 (= C202), V201 (≠ T204)
7arcG Cryo-em structure of polytomella complex-i (peripheral arm) (see paper)
32% identity, 80% coverage: 20:210/238 of query aligns to 22:208/682 of 7arcG
- binding fe2/s2 (inorganic) cluster: R36 (≠ T34), C38 (= C36), Y39 (≠ H37), G47 (= G45), C49 (= C47), R50 (= R48), C52 (= C50), C66 (= C64)
- binding iron/sulfur cluster: H98 (≠ G96), D101 (≠ V99), C102 (= C100), C105 (= C103), Q107 (≠ A105), C111 (= C109), Q114 (= Q112), C150 (= C148), I151 (= I149), C153 (= C151), C156 (= C154), C200 (= C202), V202 (≠ T204), A204 (= A206), L205 (= L207)
Sites not aligning to the query:
8e9hG Mycobacterial respiratory complex i, fully-inserted quinone (see paper)
33% identity, 87% coverage: 1:207/238 of query aligns to 2:207/782 of 8e9hG
- binding fe2/s2 (inorganic) cluster: R35 (≠ T34), C37 (= C36), D38 (≠ H37), G46 (= G45), C48 (= C47), R49 (= R48), C51 (= C50), A63 (≠ P62), C65 (= C64)
- binding iron/sulfur cluster: H99 (≠ G96), D102 (≠ V99), C103 (= C100), C106 (= C103), G109 (≠ N106), C112 (= C109), Q115 (= Q112), C152 (= C148), V153 (≠ I149), L154 (= L150), C155 (= C151), A156 (≠ T152), R157 (= R153), C158 (= C154), C202 (= C202), P203 (= P203), V204 (≠ T204), A206 (= A206), L207 (= L207)
Sites not aligning to the query:
- binding guanosine-5'-triphosphate: 320, 486, 487, 554, 556, 559, 579, 580, 582, 627, 689, 695, 696, 697
- binding iron/sulfur cluster: 228, 231, 233, 235, 262, 263, 266, 391, 392
8eszS1 NADH-ubiquinone oxidoreductase chain 1 (see paper)
34% identity, 85% coverage: 8:210/238 of query aligns to 8:210/683 of 8eszS1
- binding fe2/s2 (inorganic) cluster: C36 (= C36), Y37 (≠ H37), A44 (≠ V44), G45 (= G45), C47 (= C47), R48 (= R48), C50 (= C50), C64 (= C64)
- binding iron/sulfur cluster: H96 (≠ G96), D99 (≠ V99), C100 (= C100), C103 (= C103), C109 (= C109), Q112 (= Q112), C152 (= C148), I153 (= I149), H154 (≠ L150), C155 (= C151), T156 (= T152), R157 (= R153), C158 (= C154), I182 (= I178), C202 (= C202), P203 (= P203), V204 (≠ T204)
7qsoG Bovine complex i in lipid nanodisc, state 3 (slack) (see paper)
31% identity, 88% coverage: 2:210/238 of query aligns to 3:207/700 of 7qsoG
- binding fe2/s2 (inorganic) cluster: R35 (≠ T34), C37 (= C36), Y38 (≠ H37), G46 (= G45), C48 (= C47), R49 (= R48), C51 (= C50), C65 (= C64)
- binding iron/sulfur cluster: H97 (≠ G96), D100 (≠ V99), C101 (= C100), C104 (= C103), Q106 (≠ A105), G107 (≠ N106), C110 (= C109), Q113 (= Q112), C149 (= C148), I150 (= I149), Q151 (≠ L150), C152 (= C151), T153 (= T152), R154 (= R153), C155 (= C154), C199 (= C202), P200 (= P203), V201 (≠ T204), G202 (= G205), A203 (= A206)
7v2cM Active state complex i from q10 dataset (see paper)
31% identity, 88% coverage: 2:210/238 of query aligns to 2:206/690 of 7v2cM
- binding fe2/s2 (inorganic) cluster: C36 (= C36), Y37 (≠ H37), G45 (= G45), C47 (= C47), R48 (= R48), C50 (= C50), C64 (= C64)
- binding magnesium ion: Q105 (≠ A105), C198 (= C202), V200 (≠ T204)
- binding iron/sulfur cluster: H96 (≠ G96), D99 (≠ V99), C100 (= C100), C103 (= C103), Q105 (≠ A105), C109 (= C109), Q112 (= Q112), C148 (= C148), C151 (= C151), T152 (= T152), R153 (= R153), C154 (= C154), C198 (= C202), V200 (≠ T204), A202 (= A206), L203 (= L207)
5gupG structure of mammalian respiratory supercomplex I1III2IV1 (see paper)
31% identity, 88% coverage: 2:210/238 of query aligns to 1:205/673 of 5gupG
- binding fe2/s2 (inorganic) cluster: C35 (= C36), Y36 (≠ H37), N45 (≠ A46), C46 (= C47), C49 (= C50), C63 (= C64)
- binding iron/sulfur cluster: H95 (≠ G96), C99 (= C100), C102 (= C103), Q104 (≠ A105), C108 (= C109), Q111 (= Q112), R146 (= R147), C147 (= C148), I148 (= I149), C150 (= C151), T151 (= T152), C153 (= C154), V177 (≠ I178), C197 (= C202), P198 (= P203), A201 (= A206), L202 (= L207)
Sites not aligning to the query:
5lc5G Structure of mammalian respiratory complex i, class2 (see paper)
31% identity, 86% coverage: 3:207/238 of query aligns to 1:201/685 of 5lc5G
- binding fe2/s2 (inorganic) cluster: R32 (≠ T34), C34 (= C36), G43 (= G45), C45 (= C47), C48 (= C50), C62 (= C64)
- binding iron/sulfur cluster: H94 (≠ G96), D97 (≠ V99), C98 (= C100), C101 (= C103), C107 (= C109), Q110 (= Q112), C146 (= C148), I147 (= I149), C149 (= C151), C152 (= C154), C196 (= C202), G199 (= G205)
4hea3 Crystal structure of the entire respiratory complex i from thermus thermophilus (see paper)
32% identity, 87% coverage: 3:208/238 of query aligns to 1:215/756 of 4hea3
- binding fe2/s2 (inorganic) cluster: C34 (= C36), S35 (≠ H37), G43 (= G45), C45 (= C47), R46 (= R48), C48 (= C50), C66 (= C64)
- binding iron/sulfur cluster: H98 (≠ G96), D101 (≠ V99), C102 (= C100), C105 (= C103), C111 (= C109), Q114 (= Q112), R159 (= R147), C160 (= C148), I161 (= I149), C163 (= C151), R165 (= R153), C166 (= C154), F181 (≠ V169), C209 (= C202), P210 (= P203), V211 (≠ T204), A213 (= A206), L214 (= L207)
Sites not aligning to the query:
- active site: 355, 356, 488, 516, 517
- binding iron/sulfur cluster: 235, 237, 238, 240, 241, 242, 270, 273, 387
3i9vC Crystal structure of the hydrophilic domain of respiratory complex i from thermus thermophilus, oxidized, 2 mol/asu (see paper)
32% identity, 87% coverage: 3:208/238 of query aligns to 1:213/754 of 3i9vC
- binding calcium ion: D142 (= D135), H145 (= H138)
- binding fe2/s2 (inorganic) cluster: C34 (= C36), S35 (≠ H37), G43 (= G45), A44 (= A46), C45 (= C47), R46 (= R48), C48 (= C50), C66 (= C64)
- binding iron/sulfur cluster: H98 (≠ G96), D101 (≠ V99), C102 (= C100), C105 (= C103), K107 (≠ A105), C111 (= C109), L113 (= L111), Q114 (= Q112), C158 (= C148), I159 (= I149), C161 (= C151), C164 (= C154), F179 (≠ V169), I188 (≠ V179), C207 (= C202), P208 (= P203), V209 (≠ T204), A211 (= A206)
Sites not aligning to the query:
- active site: 270, 353, 354, 486, 514, 515
- binding manganese (ii) ion: 251, 279
- binding iron/sulfur cluster: 233, 235, 236, 238, 239, 240, 268, 271, 384
3i9v3 Crystal structure of the hydrophilic domain of respiratory complex i from thermus thermophilus, oxidized, 2 mol/asu (see paper)
32% identity, 87% coverage: 3:208/238 of query aligns to 1:213/754 of 3i9v3
- binding fe2/s2 (inorganic) cluster: C34 (= C36), S35 (≠ H37), G43 (= G45), C45 (= C47), R46 (= R48), C48 (= C50), C66 (= C64)
- binding iron/sulfur cluster: H98 (≠ G96), D101 (≠ V99), C102 (= C100), C105 (= C103), C111 (= C109), Q114 (= Q112), C158 (= C148), I159 (= I149), C161 (= C151), C164 (= C154), F179 (≠ V169), I188 (≠ V179), C207 (= C202), P208 (= P203), V209 (≠ T204), A211 (= A206)
Sites not aligning to the query:
- active site: 353, 354, 486, 514, 515
- binding manganese (ii) ion: 251, 279
- binding iron/sulfur cluster: 233, 236, 238, 239, 240, 268, 271, 384
Query Sequence
>Synpcc7942_2557 FitnessBrowser__SynE:Synpcc7942_2557
MSVVTLQIDDQELAANVGQTVLQVAREASIPIPTLCHLQGVSDVGACRLCVVEVAGSPKL
QPACLLTVSEGLVVQTRSPRLERYRRQIVELFFAEGNHVCAICVANGNCELQDAAIAVGM
DHSRYPYRFPKRDVDLSHRFFGLDHNRCILCTRCVRVCDEIEGAHVWDVAMRGEHCRIVA
GMDQPWGAVDACTNCGKCIDACPTGALFHKGETTGEIERDRDKLAFLAEARGQRRWTR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory