SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Synpcc7942_B2623 FitnessBrowser__SynE:Synpcc7942_B2623 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
51% identity, 94% coverage: 4:312/329 of query aligns to 3:309/310 of P9WP55

query
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P9WP55

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K44 (= K47) modified: N6-(pyridoxal phosphate)lysine
N74 (= N77) binding
GTGGT 178:182 (= GTGGT 181:185) binding
S266 (= S269) binding

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
51% identity, 93% coverage: 4:309/329 of query aligns to 3:306/306 of 2q3dA

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2q3dA

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active site: K44 (= K47), S266 (= S269), P293 (= P296)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K47), T71 (= T74), S72 (≠ T75), N74 (= N77), T75 (= T78), Q144 (= Q147), V177 (= V180), G178 (= G181), T179 (= T182), G180 (= G183), T182 (= T185), G222 (= G225), I223 (≠ M226), S266 (= S269), P293 (= P296), D294 (≠ S297)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
51% identity, 91% coverage: 4:303/329 of query aligns to 3:300/300 of 3zeiA

query
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3zeiA

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active site: K44 (= K47), S266 (= S269), P293 (= P296)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T74), S72 (≠ T75), I126 (= I129), Q144 (= Q147), F145 (= F148), K215 (≠ A218), G222 (= G225), A225 (≠ P228), F227 (= F230)
binding pyridoxal-5'-phosphate: K44 (= K47), N74 (= N77), V177 (= V180), G178 (= G181), T179 (= T182), G180 (= G183), T182 (= T185), G222 (= G225), S266 (= S269), P293 (= P296), D294 (≠ S297)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
51% identity, 91% coverage: 4:303/329 of query aligns to 3:300/300 of 2q3cA

query
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2q3cA

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I

V

V

A

V

V

V

C

L

P
|
P

S

D

G

F

S

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K47), S266 (= S269), P293 (= P296)
binding : T71 (= T74), S72 (≠ T75), G73 (= G76), T75 (= T78), M122 (= M125), Q144 (= Q147), K215 (≠ A218), G222 (= G225), A225 (≠ P228)

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
48% identity, 95% coverage: 4:314/329 of query aligns to 13:322/323 of 4aecA

query
sites
4aecA

I

I

L

A

N

D

R

N

L

V

T

S

E

Q

W

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

R

Y

L

L

Q

N

E

S

T

I

A

A

Q

K

Q

-

Y

-

G

G

A

C

I

V

A

A

E

N

V

I

L

A

K

K

L

L

E

E

Y

I

L

M

N

E

P

P

L

C

G

C

S

S

V

V

K
|
K

D

D

R

R

T

I

A

G

L

Y

S

S

L

M

I

V

Q

T

S

D

A

A

R

E

S

Q

Q

K

G

G

L

F

I

I

T

S

A

P

G

G

-

K

T

S

T

V

L

L

I

V

E

E

P

P

T

T

T

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

F

F

A

I

A

A

A

S

E

R

Q

G

L

Y

R

R

L

L

I

I

L

L

V

T

M

M

P

P

D

A

R

S

V

M

S

S

A

M

E

E

R

R

I

R

R

V

L

L

A

L

K

K

L

A

L

F

G

G

A

A

K

E

V

L

V

V

L

L

T

T

P

D

A

P

A

A

E

K

A

G

M

M

L

T

G

G

C

A

I

V

A

Q

K

K

A

A

Q

E

A

E

L

I

Q

L

Q

K

Q

N

I

T

P

P

D

D

S

A

V

Y

I

M

L

L

Q

Q

F

F

Q

D

N

N

P

P

A

A

N

N

P

P

S

K

I

I

H

H

Q

Y

A

E

T

T

G

G

P

P

E

E

I

I

W

W

R

D

D

D

T

T

D

K

G

G

E

K

V

V

D

D

I

I

V

F

V

V

A

A

G

G

V
x
I

G
|
G

T
|
T

G
|
G

T
|
T

L

I

M

T

G

G

I

V

S

G

R

R

Y

F

L

I

K

K

P

E

L

K

R

N

P

P

R

K

L

T

Q

Q

S

V

I

I

A

G

V

V

Q

E

P

P

A

T

R

E

S

S

P

D

V

I

L

L

S

S

G

G

A

K

A

P

G

G

S

P

H

H

Q

K

L

I

T

Q

G
|
G

M

I

G

G

P

A

N

G

F

F

V

I

S

P

P

K

L

N

V

L

D

D

R

Q

S

K

L

I

I

M

D

D

E

E

I

V

L

I

S

A

A

I

Y

S

E

S

E

E

D

E

A

A

I

I

A

E

V

T

I

A

R

K

T

Q

C

L

A

A

A

L

R

K

E

E

G

G

I

L

P

M

L

V

G

G

V

I

S
|
S

S

S

G

G

A

A

I

A

V

A

W

A

A

A

L

I

Q

K

V

V

A

A

K

K

R

R

P

P

E

E

N

N

A

A

G

G

K

K

R

L

I

I

V

A

A

V

V

V

C

F

P
|
P

S

S

G

F

S

G

E

E

R

R

Y

Y

L

L

S

S

S

T

T

P

L

L

L

F

A

Q

D

S

I

I

S

R

L

E

E

E

active site: K54 (= K47), S277 (= S269)
binding pyridoxal-5'-phosphate: K54 (= K47), N85 (= N77), I188 (≠ V180), G189 (= G181), T190 (= T182), G191 (= G183), G192 (= G184), T193 (= T185), G233 (= G225), S277 (= S269), P304 (= P296)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
47% identity, 94% coverage: 3:311/329 of query aligns to 2:311/318 of 4lmaA

query
sites
4lmaA

K

R

I

I

L

A

N

H

R

D

L

V

T

T

E

E

W

L

I

V

G

G

N

R

T

T

P

P

L

L

V

V

R

Q

L

L

Q

N

E

R

T

I

A

P

Q

Q

Q

A

Y

E

G

G

A

C

I

L

A

G

E

R

V

I

L

V

L

M

K

K

L

L

E

E

Y

G

L

M

N

N

P

P

L

A

G

A

S

S

V

V

K
|
K

D

D

R

R

T

I

A

G

L

T

S

H

L

M

I

I

Q

N

S

S

A

A

R

E

S

K

Q

A

G

G

L

L

I

I

T

N

A

P

G

E

T

T

-

V

L

L

I

V

E

E

P

P

T

T

T

S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

A

A

F

M

A

T

A

A

E

K

Q

G

L

Y

R

R

L

L

I

I

L

L

V

T

M

M

P

P

D

E

R

T

V

M

S

S

A

L

E

E

R

R

I

R

R

A

L

M

A

L

K

K

L

A

L

Y

G

G

A

A

K

T

V

L

V

E

L

L

T

T

P

P

A

G

A

S

E

Q

A

G

M

M

L

K

G

G

C

A

I

I

A

L

K

R

A

A

Q

Q

A

Q

L

I

Q

V

Q

D

Q

S

I

I

P

P

D

G

S

A

V

Y

I

M

L

L

Q

Q

F

F

Q

R

N

N

P

P

A

S

N

N

P

P

S

E

I

I

H

H

Q

R

A

L

T

T

G

A

P

E

E

E

I

I

W

W

R

Q

D

D

T

T

D

E

G

G

E

Q

V

V

D

D

I

F

V

I

V

V

A

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

M

T

G

G

I

V

S

A

R

E

Y

V

L

I

K

K

P

S

L

R

R

K

P

P

R

S

L

F

Q

Q

S

V

I

V

A

A

V

V

Q

E

P

P

A

F

R

N

S

S

P

P

V

V

L

I

S

S

G

G

A

N

A

P

G

G

S

P

H

H

Q

K

L

I

T

Q

G
|
G

M

I

G

G

P

A

N

G

F

F

V

I

S

P

P

E

L

V

V

L

D

R

R

T

S

D

L

L

I

I

D

D

E

E

I

V

L

I

S

T

A

V

Y

S

E

D

E

E

D

E

A

A

I

F

A

Q

V

F

I

G

R

R

T

R

C

L

A

A

A

K

R

E

E

E

G

G

I

L

P

L

L

S

G

G

V

I

S
|
S

S

S

G

G

A

A

I

N

V

L

W

C

A

A

L

I

Q

Q

V

L

A

A

K

Q

R

R

P

P

E

E

N

N

A

E

G

G

K

K

R

L

I

I

V

V

A

V

V

I

C

Q

P
|
P

S

S

G

F

S

G

E

E

R

R

Y

Y

L

L

S

S

S

T

T

L

L

M

L

F

A

Q

D

N

I

I

active site: K46 (= K47), S269 (= S269)
binding pyridoxal-5'-phosphate: K46 (= K47), N77 (= N77), G181 (= G181), T182 (= T182), G183 (= G183), T185 (= T185), G225 (= G225), S269 (= S269), P296 (= P296)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
49% identity, 94% coverage: 4:311/329 of query aligns to 3:309/309 of 7n2tA

query
sites
7n2tA

I

I

L

A

N

K

R

D

L

V

T

T

E

E

W

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

R

Y

L

L

Q

N

E

R

T

V

A

V

Q

D

Q

-

Y

-

G

G

A

C

I

V

A

A

E

R

V

V

L

A

K

K

L

L

E

E

Y

M

L

M

N

E

P

P

L

C

G

S

S

S

V

V

K

K

D

D

R

R

T

I

A

G

L

Y

S

S

L

M

I

I

Q

S

S

D

A

A

R

E

S

N

Q

K

G

G

L

L

I

I

T

T

A

P

G

G

-

E

T

S

T

V

L

L

I

I

E

E

P

P

T

T

T

S

G

G

N

N

T

T

G

G

I

I

G

G

L

L

A

A

F

F

A

I

A

A

E

K

Q

G

L

Y

R

R

L

L

I

I

L

I

V

C

M

M

P

P

D

A

R

S

V

M

S

S

A

L

E

E

R

R

I

R

R

T

L

I

A

L

K

R

L

A

L

F

G

G

A

A

K

E

V

L

V

V

L

L

T

T

P

D

A

P

A

A

E

R

A

G

M

M

L

K

G

G

C

A

I

V

A

Q

K

K

A

A

Q

E

A

E

L

I

Q

K

Q

A

Q

K

I

T

P

P

D

N

S

S

V

Y

I

I

L

L

Q

Q

F

F

Q

E

N

N

P

P

A

A

N

N

P

P

S

K

I

I

H

H

Q

Y

A

E

T

T

G

G

P

P

E

E

I

I

W

W

R

R

D

G

T

S

D

G

G

G

E

K

V

I

D

D

I

A

V

L

V

V

A

S

G

G

V

I

G

G

T

T

G

G

T

T

L

V

M

T

G

G

I

A

S

G

R

K

Y

Y

L

L

K

K

P

E

L

Q

R

N

P

P

R

N

L

I

Q

K

S

L

I

Y

A

G

V

V

Q

E

P

P

A

V

R

E

S

S

P

A

V

I

L

L

S

S

G

G

A

K

A

P

G

G

S

P

H

H

Q

K

L

I

T

Q

G

G

M

I

G

G

P

A

N

G

F

F

V

I

S

P

P

G

L

V

V

L

D

D

R

V

S

N

L

L

I

L

D

D

E

E

I

V

L

I

S

Q

A

V

Y

S

E

S

E

E

D

E

A

S

I

I

A

E

V

T

I

A

R

K

T

L

C

L

A

A

A

L

R

K

E

E

G

G

I

L

P

L

L

V

G

G

V

I

S

S

G

G

A

A

I

A

V

A

W

A

A

A

L

I

Q
x
R

V

I

A

A

K

K

R
|
R

P

P

E

E

N

N

A

A

G

G

K

K

R

L

I

I

V

V

A

A

V

V

C

F

P

P

S

S

G

F

S

G

E

E

R

R

Y

Y

L

L

S

S

S

T

T

V

L

L

L

F

A

E

D

S

I

V

binding phosphate ion: R277 (≠ Q279), R281 (= R283)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
48% identity, 96% coverage: 4:318/329 of query aligns to 75:388/392 of P47999

query
sites
P47999

I

I

L

A

N

D

R

N

L

A

T

A

E

Q

W

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

R

Y

L

L

Q

N

E

N

T

V

A

V

Q

K

Q

-

Y

-

G

G

A

C

I

V

A

A

E

S

V

V

L

A

K

K

L

L

E

E

Y

I

L

M

N

E

P

P

L

C

G

C

S

S

V

V

K

K

D

D

R

R

T

I

A

G

L

Y

S

S

L

M

I

I

Q

T

S

D

A

A

R

E

S

E

Q

K

G

G

L

L

I

I

T

T

A

P

G

G

-

K

T

S

T

V

L

L

I

V

E

E

P

S

T

T

T

S

G

G

N

N

T

T

G

G

I

I

G

G

L

L

A

A

F

F

A

I

A

A

A

S

E

K

Q

G

L

Y

R

K

L

L

I

I

L

L

V

T

M

M

P

P

D

A

R

S

V

M

S

S

A

L

E

E

R

R

I

R

R

V

L

L

A

L

K

R

L

A

L

F

G

G

A

A

K

E

V

L

V

V

L

L

T

T

P

E

A

P

A

A

E

K

A

G

M

M

L

T

G

G

C

A

I

I

A

Q

K

K

A

A

Q

E

A

E

L

I

Q

L

Q

K

I

T

P

P

D

N

S

S

V

Y

I

M

L

L

Q

Q

F

F

Q

D

N

N

P

P

A

A

N

N

P

P

S

K

I

I

H

H

Q

Y

A

E

T

T

G

G

P

P

E

E

I

I

W

W

R

E

D

D

T

T

D

R

G

G

E

K

V

I

D

D

I

I

V

L

V

V

A

A

G

G

V

I

G

G

T

T

G

G

T

T

L

I

M

T

G

G

I

V

S

G

R

R

Y

F

L

I

K

K

P

E

L

R

R

K

P

P

R

E

L

L

Q

K

S

V

I

I

A

G

V

V

Q

E

P

P

A

T

R

E

S

S

P

A

V

I

L

L

S

S

G

G

A

K

A

P

G

G

S

P

H

H

Q

K

L

I

T

Q

G

G

M

I

G

G

P

A

N

G

F

F

V

V

S

P

P

K

L

N

V

L

D

D

R

L

S

A

L

I

I

V

D

D

E

E

I

Y

L

I

S

A

A

I

Y

S

E

S

E

E

D

E

A

A

I

I

A

E

V

T

I

S

R

K

T

Q

C

L

A

A

A

L

R

Q

E

E

G

G

I

L

P

L

L

V

G

G

V

I

S

S

G

G

A

A

I

A

V

A

W

A

A

A

L

I

Q

Q

V

V

A

A

K

K

R

R

P

P

E

E

N

N

A

A

G

G

K

K

R

L

I

I

V

A

A

V

V

V

C

F

P

P

S

S

G

F

S

G

E

E

R

R

Y

Y

L

L

S

S

S

T

T

Q

L

L

L

F

A

Q

D

S

I

I

S

R

L

E

E

E

S

C

D

E

D

Q

V

M

Sites not aligning to the query:

61 modified: N-acetylalanine

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
48% identity, 93% coverage: 4:310/329 of query aligns to 3:310/310 of 4lmbA

query
sites
4lmbA

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active site: K46 (= K47), S269 (= S269)
binding cysteine: K46 (= K47), T74 (= T74), S75 (≠ T75), N77 (= N77), T78 (= T78), M101 (≠ V101), M125 (= M125), M125 (= M125), Q147 (= Q147), F148 (= F148), Q224 (≠ T224), G225 (= G225), G225 (= G225), I226 (≠ M226), A228 (≠ P228)
binding pyridoxal-5'-phosphate: K46 (= K47), N77 (= N77), V180 (= V180), G181 (= G181), T182 (= T182), G183 (= G183), T185 (= T185), G225 (= G225), S269 (= S269), P296 (= P296)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
49% identity, 95% coverage: 1:311/329 of query aligns to 2:309/310 of 5xoqA

query
sites
5xoqA

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binding : T72 (= T74), S73 (≠ T75), G74 (= G76), T76 (= T78), M123 (= M125), Q144 (= Q147), R218 (≠ S220), H219 (= H221), Q222 (≠ T224), G223 (= G225), A226 (≠ P228)

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
47% identity, 93% coverage: 3:308/329 of query aligns to 2:306/320 of 2isqA

query
sites
2isqA

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active site: K44 (= K47), S267 (= S269)
binding pyridoxal-5'-phosphate: K44 (= K47), N75 (= N77), G177 (= G179), G179 (= G181), T180 (= T182), G181 (= G183), T183 (= T185), G223 (= G225), S267 (= S269), P294 (= P296)
binding : T72 (= T74), S73 (≠ T75), G74 (= G76), T76 (= T78), G122 (≠ A124), M123 (= M125), K124 (≠ L126), G217 (= G219), P218 (≠ S220), H219 (= H221), Q222 (≠ T224), G223 (= G225)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
47% identity, 93% coverage: 3:308/329 of query aligns to 4:308/322 of P47998

query
sites
P47998

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G

K

K

R

L

I

F

V

V

A

A

V

I

C

F

P

P

S

S

G

F

S

G

E

E

R

R

Y

Y

L

L

S

S

S

T

T

V

L

L

L

F

K46 (= K47) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T74) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (≠ T75) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N77) binding ; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T78) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q147) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H157) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G162) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (= GTGGT 181:185) binding
T182 (= T182) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T185) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ A217) mutation to A: Impaired interaction with SAT1.
H221 (= H221) mutation to A: Impaired interaction with SAT1.
K222 (≠ Q222) mutation to A: Impaired interaction with SAT1.
S269 (= S269) binding ; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

3vbeC Crystal structure of beta-cyanoalanine synthase in soybean (see paper)
44% identity, 96% coverage: 3:318/329 of query aligns to 10:324/329 of 3vbeC

query
sites
3vbeC

K

N

I

I

L

K

N

K

R

H

L

V

T

S

E

Q

W

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

R

Y

L

L

Q

N

E

K

T

V

A

T

Q

E

Q

G

Y

C

G

G

A

A

I

Y

A

-

E

-

V

V

L

A

L

V

K

K

L

Q

E

E

Y

M

L

M

N

Q

P

P

L

T

G

A

S

S

V

I

K
|
K

D

D

R

R

T

P

A

A

L

Y

S

A

L

M

I

I

Q

T

S

D

A

A

R

E

S

E

Q

K

G

N

L

L

I

I

T

T

A

P

G

G

-

K

T

T

L

L

I

I

E

E

P

P

T

T

T
x
S

G

G

N
|
N

T

M

G

G

I

I

G

S

L

M

A

A

F

F

A

M

A

A

A

M

E

K

Q

G

L

Y

R

K

L

M

I

V

L

L

V

T

M

M

P

P

D

S

R

Y

V

T

S

S

A

L

E

E

R

R

I

R

R

V

L

T

A

M

K

R

L

A

L

F

G

G

A

A

K

E

V

L

V

I

L

L

T

T

P

D

A

P

A

A

E

K

A

G

M

M

L

G

G

G

C

T

I

V

A

K

K

K

A

A

Q

Y

A

E

L

L

Q

L

Q

E

Q

N

I

T

P

P

D

N

S

A

V

H

I

M

L

L

Q

Q

F

F

Q

S

N

N

P

P

A

A

N

N

P

T

S

Q

I

V

H

H

Q

F

A

E

T

T

G

G

P

P

E

E

I

I

W

W

R

E

D

D

T

T

D

N

G

G

E

Q

V

V

D

D

I

I

V

F

V

V

A
x
M

G

G

V

I

G
|
G

T
x
S

G
|
G

G

G

T
|
T

L

V

M

S

G

G

I

V

S

G

R

Q

Y

Y

L

L

K

K

P

S

L

K

R

N

P

P

R

N

L

V

Q

K

S

I

I

Y

A

G

V

V

Q
x
E

P

P

A

S

R

E

S
|
S

P

N

V

V

L

L

S

N

G

G

A

K

A

P

G

G

S

P

H

H

Q

H

L

I

T

T

G
|
G

M

N

G

G

P

V

N

G

F

F

V

K

S

P

P

D

L

I

V

L

D

D

R

L

S

D

L

V

I

M

D

E

E

K

I

V

L

L

S

E

A

V

Y

S

E

S

E

E

D

D

A

A

I

V

A

N

V

M

I

A

R

R

T

V

C

L

A

A

A

L

R

K

E

E

G

G

I

L

P

M

L

V

G

G

V

I

S
|
S

S

S

G

G

A

A

I

N

V

T

W

V

A

A

L

L

Q

R

V

L

A

A

K

Q

R

L

P

P

E

E

N

N

A

K

G

G

K

K

R

L

I

I

V

V

A

T

V

V

C

H

P
|
P

S

S

G

F

S

G

E

E

R

R

Y

Y

L

L

S

S

T

V

L

L

L

F

A

Q

D

E

I

L

S

R

L

Q

E

E

S

A

D

E

D

N

V

M

active site: K52 (= K47), S81 (≠ T75), E212 (≠ Q206), S216 (= S210), S275 (= S269), P302 (= P296)
binding pyridoxal-5'-phosphate: K52 (= K47), N83 (= N77), M184 (≠ A178), G187 (= G181), S188 (≠ T182), G189 (= G183), T191 (= T185), G231 (= G225), S275 (= S269), P302 (= P296)

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
46% identity, 93% coverage: 3:308/329 of query aligns to 2:306/320 of 1z7yA

query
sites
1z7yA

K

R

I

I

L

A

N

K

R

D

L

V

T

T

E

E

W

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

R

Y

L

L

Q

N

E

N

T

V

A

A

Q

E

Q

-

Y

-

G

G

A

C

I

V

A

G

E

R

V

V

L

A

K

K

L

L

E

E

Y

M

L

M

N

E

P

P

L

C

G

S

S

S

V

V

K
x
A

D

D

R

R

T

I

A

G

L

F

S

S

L

M

I

I

Q

S

S

D

A

A

R

E

S

K

Q

K

G

G

L

L

I

I

T

K

A

P

G

G

-

E

T

S

T

V

L

L

I

I

E

E

P

P

T

T

T

S

G
|
G

N
|
N

T
|
T

G

G

I

V

G

G

L

L

A

A

F

F

A

T

A

A

E

K

Q

G

L

Y

R

K

L

L

I

I

L

I

V

T

M

M

P

P

D

A

R

S

V

M

S

S

A

T

E

E

R

R

I

R

R

I

L

I

A

L

K

L

L

A

L

F

G

G

A

V

K

E

V

L

V

V

L

L

T

T

P

D

A

P

A

A

E

K

A

G

M

M

L

K

G

G

C

A

I

I

A

A

K

K

A

A

Q

E

A

E

L

I

Q

L

Q

A

Q

K

I

T

P

P

D

N

S

G

V

Y

I

M

L

L

Q

Q

Q
|
Q

F

F

Q

E

N

N

P

P

A

A

N

N

P

P

S

K

I

I

H

H

Q

Y

A

E

T

T

G

G

P

P

E

E

I

I

W

W

R

K

D

G

T

T

D

G

G

G

E

K

V

I

D

D

I

G

V

F

V

V

A

S

G

G

V
x
I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

M

T

G

G

I

A

S

G

R

K

Y

Y

L

L

K

K

P

E

L

Q

R

N

P

A

R

N

L

V

Q

K

S

L

I

Y

A

G

V

V

Q

E

P

P

A

V

R

E

S

S

P

A

V

I

L

L

S

S

G

G

A

K

A

P

G

G

S

P

H

H

Q

K

L

I

T

Q

G
|
G

M

I

G

G

P

A

N

G

F

F

V

I

S

P

P

S

L

V

V

L

D

N

R

V

S

D

L

L

I

I

D

D

E

E

I

V

L

V

S

Q

A

V

Y

S

E

S

E

D

D

E

A

S

I

I

A

D

V

M

I

A

R

R

T

Q

C

L

A

A

A

L

R

K

E

E

G

G

I

L

P

L

L

V

G

G

V

I

S
|
S

S

S

G

G

A

A

I

A

V

A

W

A

A

A

L

I

Q

K

V

L

A

A

K

Q

R

R

P

P

E

E

N

N

A

A

G

G

K

K

R

L

I

F

V

V

A

A

V

I

C

F

P
|
P

S
|
S

G

F

S

G

E

E

R

R

Y

Y

L

L

S

S

S

T

T

V

L

L

L

F

active site: A44 (≠ K47), S267 (= S269)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G76), N75 (= N77), T76 (= T78), Q145 (= Q147), I178 (≠ V180), G179 (= G181), T180 (= T182), G181 (= G183), T183 (= T185), G223 (= G225), S267 (= S269), P294 (= P296), S295 (= S297)

3vc3A Crystal structure of beta-cyanoalanine synthase k95a mutant in soybean (see paper)
44% identity, 96% coverage: 3:318/329 of query aligns to 3:317/322 of 3vc3A

query
sites
3vc3A

K

N

I

I

L

K

N

K

R

H

L

V

T

S

E

Q

W

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

R

Y

L

L

Q

N

E

K

T

V

A

T

Q

E

Q

G

Y

C

G

G

A

A

I

Y

A

-

E

-

V

V

L

A

L

V

K

K

L

Q

E

E

Y

M

L

M

N

Q

P

P

L

T

G

A

S

S

V

I

K
x
A

D

D

R

R

T

P

A

A

L

Y

S

A

L

M

I

I

Q

T

S

D

A

A

R

E

S

E

Q

K

G

N

L

L

I

I

T

T

A

P

G

G

-

K

T

T

L

L

I

I

E

E

P

P

T
|
T

T
x
S

G

G

N
|
N

T
x
M

G

G

I

I

G

S

L

M

A

A

F

F

A

M

A

A

A

M

E

K

Q

G

L

Y

R

K

L

M

I

V

L

L

V

T

M

M

P

P

D

S

R

Y

V

T

S

S

A

L

E

E

R

R

I

R

R

V

L

T

A

M

K

R

L

A

L

F

G

G

A

A

K

E

V

L

V

I

L

L

T

T

P

D

A

P

A

A

E

K

A

G

M

M

L

G

G

G

C

T

I

V

A

K

K

K

A

A

Q

Y

A

E

L

L

Q

L

Q

E

Q

N

I

T

P

P

D

N

S

A

V

H

I

M

L

L

Q

Q

Q
|
Q

F

F

Q

S

N

N

P

P

A

A

N

N

P

T

S

Q

I

V

H

H

Q

F

A

E

T

T

G

G

P

P

E

E

I

I

W

W

R

E

D

D

T

T

D

N

G

G

E

Q

V

V

D

D

I

I

V

F

V

V

A
x
M

G

G

V

I

G
|
G

T
x
S

G
|
G

G

G

T
|
T

L

V

M

S

G

G

I

V

S

G

R

Q

Y

Y

L

L

K

K

P

S

L

K

R

N

P

P

R

N

L

V

Q

K

S

I

I

Y

A

G

V

V

Q

E

P

P

A

S

R

E

S

S

P

N

V

V

L

L

S

N

G

G

A

K

A

P

G

G

S

P

H

H

Q

H

L

I

T

T

G
|
G

M

N

G

G

P

V

N

G

F

F

V

K

S

P

P

D

L

I

V

L

D

D

R

L

S

D

L

V

I

M

D

E

E

K

I

V

L

L

S

E

A

V

Y

S

E

S

E

E

D

D

A

A

I

V

A

N

V

M

I

A

R

R

T

V

C

L

A

A

A

L

R

K

E

E

G

G

I

L

P

M

L

V

G

G

V

I

S
|
S

S

S

G

G

A

A

I

N

V

T

W

V

A

A

L

L

Q

R

V

L

A

A

K

Q

R

L

P

P

E

E

N

N

A

K

G

G

K

K

R

L

I

I

V

V

A

T

V

V

C

H

P
|
P

S

S

G

F

S

G

E

E

R

R

Y

Y

L

L

S

S

T

V

L

L

L

F

A

Q

D

E

I

L

S

R

L

Q

E

E

S

A

D

E

D

N

V

M

active site: A45 (≠ K47), S268 (= S269), P295 (= P296)
binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-cysteine: T73 (= T74), S74 (≠ T75), N76 (= N77), M77 (≠ T78), Q146 (= Q147), M177 (≠ A178), G180 (= G181), S181 (≠ T182), G182 (= G183), T184 (= T185), G224 (= G225), S268 (= S269), P295 (= P296)

Q93244 Cysteine synthase 1; O-acetylserine (thiol)-lyase 1; OAS-TL; EC 2.5.1.47 from Caenorhabditis elegans (see 2 papers)
48% identity, 91% coverage: 12:311/329 of query aligns to 15:313/341 of Q93244

query
sites
Q93244

I

I

G

G

N

N

T

T

P

P

L

M

V

V

R

Y

L

I

Q

N

E

K

T

L

A

T

Q

K

Q

-

Y

-

G

G

A

L

I

P

A

G

E

T

V

V

L

A

L

V

K

K

L

I

E

E

Y

Y

L

M

N

N

P

P

L

A

G

G

S

S

V

V

K

K

D

D

R

R

T

I

A

G

L

A

S

A

L

M

I

L

Q

A

S

A

A

A

R

E

S

K

Q

D

G

G

L

T

I

V

T

I

A

P

G

G

-

V

T

T

L

L

I

I

E

E

P
|
P

T

T

T

S

G

G

N

N

T

T

G

G

I

I

G

A

L

L

A

A

F

F

A

V

A
|
A

A

A

E

K

Q

G

L

Y

R

R

L

C

I

I

L

V

V

T

M

M

P

P

D

A

R

S

V

M

S

S

A

G

E

E

R

R

I

R

R

T

L

L

A

L

K

K

L

A

L

Y

G

G

A

S

K

E

V

V

L

L

T

T

P

D

A

P

A

A

E

K

A

G

M

M

L

K

G

G

C

A

I

I

A

D

K

M

A

A

Q

N

A

Q

L

L

Q

K

Q

E

Q

N

I

I

P

P

D

G

S
|
S

V

I

I

I

L

L

Q

A

Q

Q

F

F

Q

D

N

N

P

P

A

N

N

N

P

P

S

L

I

V

H

H

Q

Y

A

Q

T

T

G

G

P

P

E

E

I

I

W

W

R

R

D

Q

T

T

D

K

G

G

E

T

V

V

D

D

I

A

V

V

A

F

G
|
G

V

V

G
|
G

T

T

G

G

T

T

L

I

M

T

G

G

I

V

S

G

R

R

Y

Y

L

L

K

Q

P

E

L

Q

R

N

P

P

R

G

L

V

Q

R

S

V

I

F

A

A

V

V

Q

E

P

P

A

E

R

E

S

S

P

A

V

I

L

L

S

S

G

G

G

R

A

P

A

A

G

G

S

P

H

H

Q

K

L

I

T

Q

G

G

M

I

G
|
G

P

A

N

G

F

F

V

A

S

P

P

A

L

V

V

L

D

D

R

T

S

K

L

I

I

Y

D

E

E

D

I

V

L

I

S

R

A

I

Y

H

E

S

E

D

D

E

A

A

I

I

A

V

V

M

I

A

R

Q

T
x
R

C

L

A

S

A

Y

R

E

E

E

G

G

I

L

P

L

L

G

G

G

V

I

S

S

S
|
S

G

G

A

A

I

N

V

V

W

A

A

A

L

L

Q

Q

V

L

A

A

K

A

R

R

P

P

E

E

N

M

A

A

G

G

K

K

R

L

I

I

V

V

A
x
T

V

C

C

L

P

P

S

S

G

C

S

G

E

E

R

R

Y

Y

L

M

S

T

S

S

T

P

L

L

L

Y

A

T

D

D

I

I

P75 (= P73) mutation to L: In n5537; severe loss of protein stability.
A88 (= A86) mutation to V: In n5522; severe loss of protein stability.
S144 (= S142) mutation to F: In mr26; susceptible to high levels of hydrogen sulfide.
G181 (= G179) mutation to E: In n5521 and mr23; severe loss of protein stability. Susceptible to high levels of hydrogen sulfide.
G183 (= G181) mutation to R: In n5515; severe loss of protein stability.
G229 (= G227) mutation to E: In mr33; susceptible to high levels of hydrogen sulfide.
R259 (≠ T257) mutation to K: In n5519; no loss of protein stability. No effect on enzyme activity.
S272 (= S270) mutation to F: In mr29; susceptible to high levels of hydrogen sulfide.
T295 (≠ A293) mutation to I: In mr39; susceptible to high levels of hydrogen sulfide.

Q9FS29 Bifunctional L-3-cyanoalanine synthase/cysteine synthase 2, mitochondrial; EC 2.5.1.47; EC 4.4.1.9 from Solanum tuberosum (Potato) (see paper)
43% identity, 96% coverage: 4:318/329 of query aligns to 28:341/347 of Q9FS29

query
sites
Q9FS29

I

I

L

K

N

S

R

E

L

V

T

S

E

Q

W

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

R

Y

L

L

Q

K

E

K

T

V

A

T

Q

E

Q

G

Y

C

G

G

A

A

I

Y

A

-

E

-

V

I

L

A

L

V

K

K

L

Q

E

E

Y

M

L

F

N

Q

P

P

L

T

G

S

S

S

V

I

K

K

D

D

R

R

T

P

A

A

L

L

S

A

L

M

I

I

Q

N

S

D

A

A

R

E

S

K

Q

K

G

G

L

L

I

I

T

S

-

P

A

E

G

K

T

T

L

L

I

I

E

E

P

P

T

T

T

S

G

G

N

N

T

M

G

G

I

I

G

S

L

M

A

A

F

F

A

M

A

A

A

M

E

K

Q

G

L

Y

R

K

L

M

I

V

L

L

V

T

M

M

P

P

D

S

R

Y

V

T

S

S

A

M

E

E

R

R

I

R

R

V

L

T

A

M

K

R

L

A

L

F

G

G

A

A

K

D

V

L

V

I

L

L

T

T

P

D

A

P

A

T

E

K

A

G

M

M

L

G

G

G

C

T

I

V

A

K

K

K

A

A

Q

Y

A
x
E

L

L

Q

L

Q

E

Q

S

I

T

P

P

D

N

S

A

V

F

I

M

L

L

Q

Q

F

F

Q

S

N

N

P

P

A

A

N

N

P

T

S

Q

I

V

H

H

Q

F

A

D

T

T

G

G

P

P

E

E

I

I

W

W

R

E

D

E

T

T

D

L

G

G

E

N

V

V

D

D

I

I

V

F

V

V

A

M

G

G

V

I

G

G

T

S

G

G

T

T

L

V

M

T

G

G

I

V

S

G

R

L

Y

Y

L

L

K

K

P

S

L

K

R

N

P

P

R

N

L

V

Q

K

S

I

I

Y

A

G

V

L

Q

E

P

P

A

T

R

E

S

S

P

N

V

I

L

L

S

N

G

G

A

K

A

P

G

G

S

P

H

H

Q

H

L

I

T

T

G

G

M

N

G

G

P

V

N

G

F

S

V

K

S

P

P

D

L

I

V

V

D

D

R

M

S

D

L

L

I

M

D

E

E

E

I

V

L

L

S

M

A

V

Y

S

E

S

E

E

D

D

A

A

I

V

A

N

V

M

I

A

R

R

T

E

C

L

A

A

A

V

R

K

E

E

G

G

I

L

P

M

L

V

G

G

V

I

S

S

G

G

A

A

I

N

V

T

W

V

A

A

L

L

Q

R

V

L

A

A

K

Q

R

K

P

P

E

E

N

N

A

K

G

G

K

K

R

L

I

I

V

V

A

T

V

V

C

H

P

A

S

S

G

F

S

G

E

E

R

R

Y

Y

L

L

S

S

T

V

L

L

L

Y

A

Q

D

D

I

L

S

R

L

K

E

E

S

A

D

E

D

N

V

M

E157 (≠ A134) mutation E->N,Q: No effect on catalytic activities.

8b9wA Cysteine synthase from trypanosoma theileri with plp bound (see paper)
46% identity, 92% coverage: 12:314/329 of query aligns to 17:317/329 of 8b9wA

query
sites
8b9wA

I

I

G

G

N

N

T

T

P

P

L

A

V

V

R

Y

L

L

Q

R

E

N

T

M

A

N

Q

K

Q

-

Y

-

G

-

A

T

I

A

A

A

E

T

V

I

L

V

L

L

K

K

L

L

E

E

Y

S

L

E

N

N

P

P

L

M

G

A

S

S

V

V

K
|
K

D

D

R

R

T

L

A

A

L

Y

S

A

L

I

I

Y

Q

D

S

K

A

A

R

E

S

K

Q

E

G

G

L

K

I

I

T

K

A

R

G

G

T

K

T

S

L

V

I

I

-

V

E

E

P

A

T

T

T

S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

A

A

F

L

A

L

A

G

A

A

A

V

E

R

Q

G

L

Y

R

K

L

V

I

I

L

I

V

T

M

M

P

P

D

D

R

S

V

M

S

S

A

M

E

E

R

R

I

R

R

S

L

L

A

L

K

R

L

I

L

F

G

G

A

A

K

E

V

L

V

I

L

L

T

T

P

P

A

A

A

S

E

L

A

G

M

M

L

K

G

G

C

A

I

V

A

A

K

M

A

A

Q

N

A

R

L

I

Q

V

Q

S

Q

N

I

N

P

P

D

D

S

A

V

V

I

L

L

A

Q

D

Q

Q

F

F

Q

C

N

T

P

K

A

Y

N

N

P

A

S

Q

I

I

H

H

Q

E

A

E

T

T

G

G

P

P

E

E

I

I

W

W

R

R

D

Q

T

T

D

K

G

G

E

H

V

V

D

D

I

C

V

F

V

V

A

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

L

M

T

G

G

I

V

S

A

R

R

Y

F

L

L

K

K

P

S

L

V

R

G

P

C

R

N

L

A

Q

N

S

I

I

V

A

A

V

V

Q

E

P

P

A

Q

R

E

S

S

P

P

V

V

L

L

S

S

G

G

A

R

A

P

G

G

S

P

H

H

Q

R

L

I

T

Q

G
|
G

M

I

G

G

P

A

N

G

F

F

V

V

S

P

P

E

L

V

V

L

D

D

R

R

S

E

L

L

I

V

D

D

E

E

I

I

L

I

S

Q

A

V

Y

S

E

S

E

D

D

K

A

A

I

I

A

E

V

T

I

A

R

Q

T

K

C

L

A

S

A

R

R

M

E

D

G

G

I

I

P

F

L

C

G

G

V

F

S
|
S

S

A

G

G

A

A

I

N

V

V

W

F

A

A

L

L

Q

K

V

I

A

A

K

E

R

R

P

P

E

E

N

M

A

A

G

G

K

K

R

T

I

I

V

V

A

T

V

V

C

I

P
|
P

S

S

G

F

S

G

E

E

R

R

Y

Y

L

L

S

S

S

T

T

A

L

L

L

Y

A

A

D

S

I

V

S

R

L

E

E

E

binding pyridoxal-5'-phosphate: K49 (= K47), N80 (= N77), G184 (= G181), T185 (= T182), G186 (= G183), T188 (= T185), G228 (= G225), S272 (= S269), P299 (= P296)

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
45% identity, 96% coverage: 1:316/329 of query aligns to 1:320/323 of P0ABK5

query
sites
P0ABK5

M
|
M

P

S

K

K

I

I

L

F

N

E

R

D

L

N

T

S

E

L

W

T

I

I

G

G

N

H

T

T

P

P

L

L

V

V

R

R

L

L

Q

N

E

R

T

I

A

G

Q

N

Q

-

Y

-

G

-

A

-

I

-

A

G

E

R

V

I

L

L

L

A

K

K

L

V

E

E

Y

S

L

R

N

N

P

P

L

S

G

F

S

S

V

V

K
|
K

D

C

R

R

T

I

A

G

L

A

S

N

L

M

I

I

Q

W

S

D

A

A

R

E

S

K

Q

R

G

G

L

V

I

L

T

K

A

P

G

G

T

V

T

E

L

L

I

V

E

E

P

P

T

T

T

S

G

G

N

N

T

T

G

G

I

I

G

A

L

L

A

A

F

Y

A

V

A

A

E

R

Q

G

L

Y

R

K

L

L

I

T

L

L

V

T

M

M

P

P

D

E

R

T

V

M

S

S

A

I

E

E

R

R

I

R

R

K

L

L

A

L

K

K

L

A

L

L

G

G

A

A

K

N

V

L

V

V

L

L

T

T

P

E

A

G

A

A

E

K

A

G

M

M

L

K

G

G

C

A

I

I

A

Q

K

K

A

A

Q

E

A

E

L

I

Q

V

Q

A

Q

S

I

N

P

P

D

E

S

K

-

Y

V

L

I

L

L

L

Q

Q

F

F

Q

S

N

N

P

P

A

A

N

N

P

P

S

E

I

I

H

H

Q

E

A

K

T

T

G

G

P

P

E

E

I

I

W

W

R

E

D

D

T

T

D

D

G

G

E

Q

V

V

D

D

I

V

V

F

V

I

A

A

G

G

V

V

G

G

T

T

G

G

T

T

L

L

M

T

G

G

I

V

S

S

R

R

Y

Y

L

I

K

K

P

G

L

T

R

K

P

G

R

K

-

T

-

D

L

L

Q

I

S

S

I

V

A

A

V

V

Q

E

P

P

A

T

R

D

S

S

P

P

V

V

L

I

S

A

G

Q

G

A

A

L

A

A

G

G

S

E

-

E

-

I

-

K

-

P

-

G

-

P

H

H

Q

K

L

I

T

Q

G

G

M

I

G

G

P

A

N

G

F

F

V

I

S

P

P

A

L

N

V

L

D

D

R

L

S

K

L

L

I

V

D

D

E

K

I

V

L

I

S

G

A

I

Y

T

E

N

E

E

D

E

A

A

I

I

A

S

V

T

I

A

R

R

T

R

C

L

A

M

A

E

R

E

E

E

G

G

I

I

P

L

L

A

G

G

V

I

S

S

G

G

A

A

I

A

V

V

W

A

A

A

L

L

Q

K

V

L

A

Q

K

E

R

D

P

E

E

S

N

F

A

T

G

N

K

K

R

N

I

I

V

V

A

V

V

I

C

L

P

P

S

S

G

S

S

G

E

E

R

R

Y

Y

L

L

S

S

S

T

T

A

L

L

L

F

A

A

D

D

I

L

S

F

L

T

E

E

S

K

D

E

M1 (= M1) modified: Initiator methionine, Removed
K42 (= K47) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
47% identity, 91% coverage: 12:309/329 of query aligns to 7:302/302 of 2efyA

query
sites
2efyA

I

I

G

G

N

K

T

T

P

P

L

V

V

V

R

R

L

L

Q

A

E

K

T

V

A

V

Q

E

Q

P

Y

-

G

-

A

D

I

M

A

A

E

E

V

V

L

W

L

V

K

K

L

L

E

E

Y

G

L

L

N

N

P

P

L

G

G

G

S

S

V

I

K
|
K

D

D

R

R

T

P

A

A

L

W

S

Y

L

M

I

I

Q

K

S

D

A

A

R

E

S

E

Q

R

G

G

L

I

I

L

T

R

A

P

G

G

T

S

-

G

-

Q

T

V

L

I

I

V

E

E

P

P

T
|
T

T
x
S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

F

M

A

I

A

A

A

S

E

R

Q

G

L

Y

R

R

L

L

I

I

L

L

V

T

M

M

P

P

D

A

R

Q

V

M

S

S

A

E

E

E

R

R

I

K

R

R

L

V

A

L

K

K

L

A

L

F

G

G

A

A

K

E

V

L

V

V

L

L

T

T

P

D

A

P

A

E

E

R

A

R

M

M

L

L

G

A

C

A

I

R

A

E

K

E

A

A

Q

L

A

R

L

L

Q

K

Q

E

I

L

P

-

D

G

S

A

V

F

I

M

L

P

Q

D

Q
|
Q

F

F

Q

K

N

N

P

P

A

A

N

N

P

V

S

R

I

A

H

H

Q

Y

A

E

T

T

G

G

P

P

E

E

I

L

W

Y

R

E

D

A

T

L

D

E

G

G

E

R

V

I

D

D

I

A

V

F

V

V

A
x
Y

G

G

V

S

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

M

T

G

G

I

V

S

G

R

R

Y

Y

L

L

K

K

P

E

L

R

R

I

P

P

R

H

L

V

Q

K

S

V

I

I

A

A

V

V

Q
x
E

P

P

A

A

R

R

S
|
S

P

N

V

V

L

L

S

S

G

G

A

K

A

M

G

G

S

Q

H

H

Q

G

L

F

T

Q

G
|
G

M
|
M

G

G

P
|
P

N

G

F

F

V

I

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P

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L

N

V

L

D

D

R

L

S

S

L

L

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L

D

D

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G

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Q

A

V

Y

W

E

E

D

D

A

A

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F

A

P

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L

I

A

R

R

T

R

C

L

A

A

A

R

R

E

E

E

G

G

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G

G

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M

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S

S

S

G

G

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G

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W

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A

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L

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Q

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V

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A

K

-

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G

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K

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A

active site: K40 (= K47), S70 (≠ T75), E200 (≠ Q206), S204 (= S210), S263 (= S269)
binding 5-oxohexanoic acid: T69 (= T74), G71 (= G76), T73 (= T78), Q141 (= Q147), G175 (= G181), G219 (= G225), M220 (= M226), P222 (= P228)
binding pyridoxal-5'-phosphate: K40 (= K47), N72 (= N77), Y172 (≠ A178), G175 (= G181), T176 (= T182), G177 (= G183), T179 (= T185), G219 (= G225), S263 (= S269), P289 (= P296), D290 (≠ S297)

Query Sequence

>Synpcc7942_B2623 FitnessBrowser__SynE:Synpcc7942_B2623
MPKILNRLTEWIGNTPLVRLQETAQQYGAIAEVLLKLEYLNPLGSVKDRTALSLIQSARS
QGLITAGTTLIEPTTGNTGIGLAFAAAAEQLRLILVMPDRVSAERIRLAKLLGAKVVLTP
AAEAMLGCIAKAQALQQQIPDSVILQQFQNPANPSIHQATTGPEIWRDTDGEVDIVVAGV
GTGGTLMGISRYLKPLRPRLQSIAVQPARSPVLSGGAAGSHQLTGMGPNFVSPLVDRSLI
DEILSAYEEDAIAVIRTCAAREGIPLGVSSGAIVWAALQVAKRPENAGKRIVAVCPSGSE
RYLSSTLLADISLESDDVSDWVAIPQGQA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory