SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Synpcc7942_B2664 FitnessBrowser__SynE:Synpcc7942_B2664 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
69% identity, 97% coverage: 7:326/329 of query aligns to 4:321/323 of P0ABK5

query
sites
P0ABK5

F

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F

F

A

E

D

D

N

N

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Q

L

T

T

I

I

G

G

K

H

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T

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P

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L

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L

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K

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Y

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K
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K

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C

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K

G

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L

K42 (= K48) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P0A1E3 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; EC 2.5.1.47 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
69% identity, 97% coverage: 7:326/329 of query aligns to 4:321/323 of P0A1E3

query
sites
P0A1E3

F

I

F

Y

A

E

D

D

N

N

S

S

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L

T

T

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I

G

G

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P

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L

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L

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G

G

A

-

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-

A

-

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L

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A

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R

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K

G

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L

N72 (= N78) binding
S273 (= S278) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6z4nAAA structure of oass complexed with upar inhibitor (see paper)
69% identity, 97% coverage: 7:324/329 of query aligns to 5:320/321 of 6z4nAAA

query
sites
6z4nAAA

F

I

F

Y

A

E

D

D

N

N

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S

Q

L

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T

I

I

G

G

K

H

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T

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P

L

L

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V

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R

L

L

N

N

R

R

I

I

V

G

K

N

G

G

A

-

P

-

A

-

T

R

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I

L

L

A

A

K

K

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V

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E

G

S

R

R

N

N

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P

A

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F

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K
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K

C

C

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R

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I

G

G

A

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A

N

M

M

I

I

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W

D

D

A

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P

G

G

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V

E

E

L

L

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E

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P

T
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T

S

S

G
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G

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N

T
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T

G

G

I

I

A

A

L

L

A

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Y

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L

Q

Q

Q
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F
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N

N

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A

A

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V

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G

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T

G
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G

G

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T

I

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T

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R

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Y

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I

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K

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G

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K

G

G

K

K

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A

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A

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G

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E

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K

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H

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K

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Q

G
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G

I
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I

G

G

A
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A

G

G

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F

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G

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N

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N

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K

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P

D

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S

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G

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E

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T

A

A

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L

F

F

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A

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F

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T

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E

Q

K

binding pyridoxal-5'-phosphate: K43 (= K48), N73 (= N78), V177 (= V182), G178 (= G183), T179 (= T184), G180 (= G185), T182 (= T187), G230 (= G234), S274 (= S278), P301 (= P305)
binding (1~{S},2~{S})-1-[(4-methylphenyl)methyl]-2-phenyl-cyclopropane-1-carboxylic acid: K43 (= K48), T70 (= T75), G72 (= G77), N73 (= N78), T74 (= T79), Q144 (= Q149), F145 (= F150), Q229 (= Q233), G230 (= G234), I231 (= I235), A233 (= A237)

1d6sA Crystal structure of the k41a mutant of o-acetylserine sulfhydrylase complexed in external aldimine linkage with methionine (see paper)
68% identity, 97% coverage: 7:326/329 of query aligns to 3:320/322 of 1d6sA

query
sites
1d6sA

F

I

F

Y

A

E

D

D

N

N

S

S

Q

L

T

T

I

I

G

G

K

H

T

T

P

P

L

L

V

V

R

R

L

L

N

N

R

R

I

I

V

G

K

N

G

G

A

-

P

-

A

-

T

R

V

I

L

L

A

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K

I

V

E

E

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S

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R

N

N

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P

A

S

Y

F

S

S

V

V

K
x
A

C

C

R

R

I

I

G

G

A

A

A

N

M

M

I

I

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W

D

D

A

A

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E

Q

K

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L

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L

L

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P

P

G

G

K

V

E

E

L

L

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E

P

P

T
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T

S
x
N

G

G

N
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N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

Y

V

V

A

A

R

R

G

G

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K

L

L

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L

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T

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M

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P

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S

L

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E

E

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R

K

K

L

L

A

K

A

A

Y

L

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G

A

A

K

N

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L

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V

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L

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T

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E

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K

G

G

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G

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P

D

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R

K

Y

Y

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L

L

L

Q

Q

Q
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Q

F
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F

R

S

N

N

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P

A

A

N

N

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P

A

E

I

I

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H

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K

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T

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T

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G

G

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T

I

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T

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K

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K

G

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K

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E

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K

K

P

P

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G

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P

H

H

K

K

I

I

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Q

G
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G

I

I

G

G

A

A

G

G

F

F

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I

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P

E

G

N

N

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D

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K

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K

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N

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E

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S

C

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G

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K

L

L

A

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E

S

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F

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G

N

K

K

T

N

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I

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I

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L

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P

D

S

S

S

G

G

E

E

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R

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L

L

S

S

T

T

A

A

L

L

F

F

D

A

G

D

I

L

F

F

N

T

E

E

Q

K

G

E

L

L

active site: A41 (≠ K48), G228 (= G234)
binding methionine: T68 (= T75), N69 (≠ S76), N71 (= N78), T72 (= T79), Q142 (= Q149), F143 (= F150), G176 (= G183), G228 (= G234)
binding pyridoxal-5'-phosphate: N71 (= N78), G176 (= G183), T177 (= T184), G178 (= G185), T180 (= T187), G228 (= G234), S272 (= S278), P299 (= P305)

1fcjA Crystal structure of oass complexed with chloride and sulfate (see paper)
68% identity, 92% coverage: 7:308/329 of query aligns to 3:302/302 of 1fcjA

query
sites
1fcjA

F

I

F

Y

A

E

D

D

N

N

S

S

Q

L

T

T

I

I

G

G

K

H

T

T

P

P

L

L

V

V

R

R

L

L

N

N

R

R

I

I

V

G

K

N

G

G

A

-

P

-

A

-

T

R

V

I

L

L

A

A

K

K

I

V

E

E

G

S

R

R

N

N

P

P

A

S

Y

F

S

S

V

V

K
|
K

C

C

R

R

I

I

G

G

A

A

A

N

M

M

I

I

W

W

D

D

A

A

E

E

Q

K

R

R

G

G

L

V

L

L

G

K

P

P

G

G

K

V

E

E

L

L

I

V

E

E

P

P

T

T

S

N

G
|
G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

Y

V

V

A

A

R

R

G

G

I

Y

P

K

L

L

T

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

L

I

E

E

R

R

K

K

L

L

A

K

A

A

Y

L

G

G

A

A

K

N

L

L

V

V

L

L

T

T

E

E

G

G

V

A

K

K

G

G

M

M

T

K

G

G

A

A

V

I

R

Q

R

K

A

A

E

E

D

E

I

I

A

V

A

A

S

S

D

D

P

P

D

Q

R

K

Y

Y

V

L

L

L

Q

Q

Q
|
Q

F

F

R

S

N

N

P

P

A

A

N

N

P

P

A

E

I

I

H

H

E

E

Q

K

T

T

G

G

P

P

E

E

I

I

W

W

E

E

D

D

T

T

G

D

G

G

A

Q

I

V

D

D

I

V

L

F

V

I

S

S

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

S

T

R

R

Y

Y

I

I

K

K

Q

G

T

T

Q

K

G

G

K

K

-

T

P

D

I

L

L

I

S

T

V

V

A

A

V

V

E

E

P

P

E

T

A

D

S

S

P

P

V

V

I

I

S

A

Q

Q

Q

A

R

L

S

A

G

G

L

E

P

E

L

I

K

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

G

N

N

L

L

D

D

L

L

S

K

L

L

V

I

D

D

Q

K

V

V

E

V

R

G

V

I

S

T

N

N

E

E

A

A

I

I

A

S

Y

T

A

A

R

R

L

L

A

M

Q

E

E

E

G

G

L

I

I

L

S

A

G

G

I

I

S
|
S

C

S

G

G

A

A

V

V

A

A

V

L

R

K

L

L

A

Q

Q

E

Q

D

S

E

E

S

H

F

A

T

G

N

K

K

T

N

I

I

V

V

V

I

L

L

P
|
P

D

S

S

S

G

G

active site: K41 (= K48), G228 (= G234), S272 (= S278)
binding pyridoxal-5'-phosphate: K41 (= K48), N71 (= N78), V175 (= V182), G176 (= G183), T177 (= T184), G178 (= G185), T180 (= T187), G228 (= G234), S272 (= S278), P299 (= P305)
binding sulfate ion: G70 (= G77), T72 (= T79), Q142 (= Q149)

5dbhX Crystal structure of o-acetylserine sulfhydrylase from haemophilus influenzae in complex with reaction intermediate alpha-aminoacrylate
66% identity, 95% coverage: 7:319/329 of query aligns to 2:312/312 of 5dbhX

query
sites
5dbhX

F

I

F

Y

A

A

D

D

N

N

S

S

Q

Y

T

S

I

I

G

G

K

N

T

T

P

P

L

L

V

V

R

R

L

L

N

K

R

H

I

F

V

-

K

-

G

G

A

H

P

N

A

G

T

N

V

V

L

V

A

V

K

K

I

I

E

E

G

G

R

R

N

N

P

P

A

S

Y

Y

S

S

V

V

K
|
K

C

C

R

R

I

I

G

G

A

A

A

N

M

M

I

V

W

W

D

Q

A

A

E

E

Q

K

R

D

G

G

L

T

L

L

G

T

P

K

G

G

K

K

E

E

L

I

I

V

E

D

P

A

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

Y

V

V

A

A

R

R

G

G

I

Y

P

K

L

I

T

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

L

L

E

E

R

R

R

K

K

R

L

L

A

C

A

G

Y

L

G

G

A

V

K

N

L

L

V

V

L

L

T

T

E

E

G

G

V

A

K

K

G

G

M

M

T

K

G

G

A

A

V

I

R

A

R

K

A

A

E

E

D

E

I

I

A

V

A

A

S

S

D

D

P

P

D

S

R

R

Y

Y

V

V

L

M

L

L

Q

K

Q
|
Q

F

F

R

E

N

N

P

P

A

A

N

N

P

P

A

Q

I

I

H

H

E

R

Q

E

T

T

G

G

P

P

E

E

I

I

W

W

E

K

D

D

T

T

G

D

G

G

A

K

I

V

D

D

I

V

L

V

V

V

S

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
x
S

I

I

T

T

G

G

V

I

S

S

R

R

Y

A

I

I

K

K

Q

L

T

D

Q

F

G

G

K

K

P

Q

I

I

L

T

S

S

V

V

A

A

V

V

E

E

P

P

E

V

A

E

S

S

P

P

V

V

I

I

S

S

Q

Q

Q

T

R

L

S

A

G

G

L

E

P

E

L

V

K

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

K

N

N

L

L

D

D

L

L

S

S

L

I

V

I

D

D

Q

R

V

V

E

E

R

T

V

V

S

D

N

S

E

D

E

T

A

A

I

L

A

A

Y

T

A

A

R

R

L

L

A

M

Q

A

E

E

G

G

L

I

I

L

S

A

G

G

I

I

S
|
S

C

S

G

G

A

A

V

V

A

A

V

D

R

R

L

L

A

A

Q

K

Q

L

S

P

E

E

H

F

A

A

G

D

K

K

T

L

I

I

V

V

V

I

L

L

P
|
P

D

S

S

A

G

S

E

E

R

R

Y

Y

L

L

S

S

T

T

A

A

L

L

F

F

D

E

G

G

binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: K41 (= K48), T68 (= T75), S69 (= S76), N71 (= N78), T72 (= T79), Q142 (= Q149), G176 (= G183), T177 (= T184), G178 (= G185), S180 (≠ T187), G227 (= G234), S271 (= S278), P298 (= P305)

1y7lA O-acetylserine sulfhydrylase complex (see paper)
66% identity, 95% coverage: 7:317/329 of query aligns to 2:310/310 of 1y7lA

query
sites
1y7lA

F

I

F

Y

A

A

D

D

N

N

S

S

Q

Y

T

S

I

I

G

G

K

N

T

T

P

P

L

L

V

V

R

R

L

L

N

K

R

H

I

F

V

-

K

-

G

G

A

H

P

N

A

G

T

N

V

V

L

V

A

V

K

K

I

I

E

E

G

G

R

R

N

N

P

P

A

S

Y

Y

S

S

V

V

K
|
K

C

C

R

R

I

I

G

G

A

A

A

N

M

M

I

V

W

W

D

Q

A

A

E

E

Q

K

R

D

G

G

L

T

L

L

G

T

P

K

G

G

K

K

E

E

L

I

I

V

E

D

P

A

T
|
T

S
|
S

G

G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

Y

V

V

A

A

R

R

G

G

I

Y

P

K

L

I

T

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

L

L

E

E

R

R

R

K

K

R

L

L

A

C

A

G

Y

L

G

G

A

V

K

N

L

L

V

V

L

L

T

T

E

E

G

G

V

A

K

K

G

G

M
|
M

T

K

G

G

A

A

V

I

R

A

R

K

A

A

E

E

D

E

I

I

A

V

A

A

S

S

D

D

P

P

D

S

R

R

Y

Y

V

V

L

M

L

L

Q

K

Q
|
Q

F

F

R

E

N

N

P

P

A

A

N

N

P

P

A

Q

I

I

H

H

E

R

Q

E

T

T

G

G

P

P

E

E

I

I

W

W

E

K

D

D

T

T

G

D

G

G

A

K

I

V

D

D

I

V

L

V

V

V

S

A

G

G

V

V

G

G

T

T

G

G

T

S

I

I

T

T

G

G

V

I

S

S

R

R

Y

A

I

I

K

K

Q

L

T

D

Q

F

G

G

K

K

P

Q

I

I

L

T

S

S

V

V

A

A

V

V

E

E

P

P

E

V

A

E

S

S

P

P

V

V

I

I

S

S

Q

Q

Q

T

R

L

S

A

G

G

L

E

P

E

L

V

K

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

K

N

N

L

L

D

D

L

L

S

S

L

I

V

I

D

D

Q

R

V

V

E

E

R

T

V

V

S

D

N

S

E

D

E

T

A

A

I

L

A

A

Y

T

A

A

R

R

L

L

A

M

Q

A

E

E

G

G

L

I

I

L

S

A

G

G

I

I

S
|
S

C

S

G

G

A

A

V

V

A

A

V

D

R

R

L

L

A

A

Q

K

Q

L

S

P

E

E

H

F

A

A

G

D

K

K

T

L

I

I

V

V

V

I

L

L

P
|
P

D

S

S

A

G

S

E

E

R

R

Y

Y

L

L

S

S

T

T

A

A

L

L

F

F

active site: K41 (= K48), S271 (= S278), P298 (= P305)
binding : K41 (= K48), T68 (= T75), S69 (= S76), T72 (= T79), M119 (= M126), Q142 (= Q149)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
63% identity, 95% coverage: 8:320/329 of query aligns to 5:309/310 of 5xoqA

query
sites
5xoqA

F

F

A

K

D

D

N

N

S

S

Q

E

T

S

I

I

G

G

K

R

T

T

P

P

L

L

V

V

R

Q

L

I

N

N

R

R

I

L

V

T

K

A

G

G

A

L

P

S

A

S

T

R

V

V

L

L

A

A

K

K

I

I

E

E

G

G

R

R

N

N

P

P

A

A

Y

Y

S

S

V

V

K

K

C

C

R

R

I

I

G

G

A

A

M

M

I

I

W

W

D

D

A

A

E

E

Q

Q

R

S

G

G

L

K

L

L

G

K

P

P

G

G

K

M

E

H

L

V

I

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

F

V

V

A

C

A

A

R

R

G

G

I

Y

P

K

L

L

T

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

L

I

E

E

R

R

K

M

L

M

L

L

A

K

A

S

Y

F

G

G

A

A

K

D

L

L

V

V

L

L

T

T

E

P

G

G

V

A

K

D

G

G

M
|
M

T

K

G

G

A

A

V

I

R

S

R

K

A

A

E

E

D

E

I

L

A

A

S

Q

D

-

P

-

D

P

R

G

Y

W

V

F

L

I

L

P

Q

Q

Q
|
Q

F

F

R

K

N

N

P

P

A

A

N

N

P

P

A

A

I

I

H

H

E

V

Q

K

T

T

G

G

P

P

E

E

I

I

W

W

E

N

D

D

T

T

G

E

G

G

A

Q

I

V

D

D

I

V

L

F

V

V

S

A

G

G

V

V

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

S

A

R

R

Y

F

I

L

K

K

Q

H

T

E

Q

K

G

K

K

H

P

P

I

V

L

H

S

V

V

V

A

A

V

V

E

E

P

P

E

A

A

A

S

S

P

P

V

V

I

L

S

-

Q

-

R

-

S

A

G

G

L

G

P

P

L

A

K

-

P

-

G

G

P
x
R

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

I

V

P

P

E

D

N

T

L

F

D

D

L

R

S

S

L

V

V

V

D

D

Q

E

V

I

E

L

R

S

V

V

S

T

N

D

E

D

E

E

A

A

I

I

A

E

Y

T

A

A

R

R

R

K

L

L

A

A

Q

M

E

E

G

G

L

I

I

S

S

C

G

G

I

I

S

S

C

C

G

G

A

A

V

M

A

A

A

G

A

A

V

L

R

K

L

V

A

A

Q

A

Q

R

S

P

E

E

H

F

A

A

G

G

K

K

T

T

I

I

V

V

V

T

V

V

L

L

P

P

D

D

S

A

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

A

L

L

F

F

D

E

G

N

I

L

binding : T72 (= T75), S73 (= S76), G74 (= G77), T76 (= T79), M123 (= M126), Q144 (= Q149), R218 (≠ P229), H219 (= H230), Q222 (= Q233), G223 (= G234), A226 (= A237)

4zu6X Crystal structure of o-acetylserine sulfhydrylase from haemophilus influenzae in complex with pre-reactive o-acetyl serine, alpha- aminoacrylate reaction intermediate and peptide inhibitor at the resolution of 2.25a
66% identity, 95% coverage: 7:317/329 of query aligns to 3:309/309 of 4zu6X

query
sites
4zu6X

F

I

F

Y

A

A

D

D

N

N

S

S

Q

Y

T

S

I

I

G

G

K

N

T

T

P

P

L

L

V

V

R

R

L

L

N

K

R

H

I

F

V

-

K

-

G

G

A

H

P

N

A

G

T

N

V

V

L

V

A

V

K

K

I

I

E

E

G

G

R

R

N

N

P

P

A

S

Y

Y

S

S

V

V

K
|
K

C

C

R

R

I

I

G

G

A

A

A

N

M

M

I

V

W

W

D

Q

A

A

E

E

Q

K

R

D

G

G

L

T

L

L

G

T

P

K

G

G

K

K

E

E

L

I

I

V

E

D

P

A

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

Y

V

V

A

A

R

R

G

G

I

Y

P

K

L

I

T

T

L

L

T

T

M

M

P

P

E

E

T
|
T

M
|
M

S

S

L

L

E

E

R

R

R

K

K

R

L

L

A

C

A

G

Y

L

G

G

A

V

K

N

L

L

V

V

L

L

T

T

E

E

G

G

V

A

K
|
K

G
|
G

M

-

T

-

G

G

A

A

V

I

R

A

R

K

A

A

E

E

D

E

I

I

A

V

A

A

S

S

D

D

P

P

D

S

R

R

Y

Y

V

V

L

M

L

L

Q

K

Q
|
Q

F

F

R

E

N

N

P

P

A

A

N

N

P

P

A

Q

I

I

H

H

E

R

Q

E

T

T

G

G

P

P

E

E

I

I

W

W

E

K

D

D

T

T

G

D

G

G

A

K

I

V

D

D

I

V

L

V

V

V

S

A

G

G

V
|
V

G
|
G

T
|
T

G
|
G

T
x
S

I

I

T

T

G

G

V

I

S

S

R

R

Y

A

I

I

K

K

Q

L

T

D

Q

F

G

G

K

K

P

Q

I

I

L

T

S

S

V

V

A

A

V

V

E

E

P

P

E

V

A

E

S

S

P

P

V

V

I

I

S

S

Q

Q

Q

T

R

L

S

A

G

G

L

E

P

E

L

V

K

K

P

P

G

G

P
|
P

H
|
H

K
|
K

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

I

I

P

P

E

K

N

N

L

L

D

D

L

L

S

S

L

I

V

I

D

D

Q

R

V

V

E

E

R

T

V

V

S

D

N

S

E

D

E

T

A

A

I

L

A

A

Y

T

A

A

R

R

L

L

A

M

Q

A

E

E

G

G

L

I

I

L

S

A

G

G

I

I

S
|
S

C

S

G

G

A

A

V

V

A

A

V

D

R

R

L

L

A

A

Q

K

Q

L

S

P

E

E

H

F

A

A

G

D

K

K

T

L

I

I

V

V

V

I

L

L

P
|
P

D

S

S

A

G

S

E

E

R

R

Y

Y

L

L

S

S

T

T

A

A

L

L

F

F

binding o-acetylserine: T69 (= T75), S70 (= S76), T73 (= T79), Q141 (= Q149), G175 (= G183), G226 (= G234)
binding pyridoxal-5'-phosphate: K42 (= K48), N72 (= N78), V174 (= V182), G175 (= G183), T176 (= T184), G177 (= G185), G178 (= G186), S179 (≠ T187), G226 (= G234), S270 (= S278), P297 (= P305)
binding : S70 (= S76), T95 (= T101), M96 (= M102), K118 (= K124), G119 (= G125), P221 (= P229), H222 (= H230), K223 (= K231), A229 (= A237)

4oreX Cyrstal structure of o-acetylserine sulfhydrylase ternary complex from haemophilus influenzae at 2.2 a
66% identity, 95% coverage: 7:317/329 of query aligns to 2:308/308 of 4oreX

query
sites
4oreX

F

I

F

Y

A

A

D

D

N

N

S

S

Q

Y

T

S

I

I

G

G

K

N

T

T

P

P

L

L

V

V

R

R

L

L

N

K

R

H

I

F

V

-

K

-

G

G

A

H

P

N

A

G

T

N

V

V

L

V

A

V

K

K

I

I

E

E

G

G

R

R

N

N

P

P

A

S

Y

Y

S

S

V

V

K
|
K

C

C

R

R

I

I

G

G

A

A

A

N

M

M

I

V

W

W

D

Q

A

A

E

E

Q

K

R

D

G

G

L

T

L

L

G

T

P

K

G

G

K

K

E

E

L

I

I

V

E

D

P

A

T
|
T

S

-

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

Y

V

V

A

A

R

R

G

G

I

Y

P

K

L

I

T

T

L

L

T

T

M

M

P
|
P

E

E

T

T

M

M

S

S

L

L

E

E

R

R

R

K

K

R

L

L

A

C

A

G

Y

L

G

G

A

V

K

N

L

L

V

V

L

L

T

T

E

E

G

A

V

-

K
|
K

G
|
G

M
|
M

T

K

G

G

A

A

V

I

R

A

R

K

A

A

E

E

D

E

I

I

A

V

A

A

S

S

D

D

P

P

D

S

R

R

Y

Y

V

V

L

M

L

L

Q

K

Q

Q

F
|
F

R

E

N

N

P

P

A

A

N

N

P

P

A

Q

I

I

H

H

E

R

Q

E

T

T

G

G

P

P

E

E

I

I

W

W

E

K

D

D

T

T

G

D

G

G

A

K

I

V

D

D

I

V

L

V

V

V

S

A

G

G

V

V

G

G

T

T

G

G

T

S

I

I

T

T

G

G

V

I

S

S

R

R

Y

A

I

I

K

K

Q

L

T

D

Q

F

G

G

K

K

P

Q

I

I

L

T

S

S

V

V

A

A

V

V

E

E

P

P

E

V

A

E

S

S

P

P

V

V

I

I

S

S

Q

Q

Q

T

R

L

S

A

G

G

L

E

P

E

L

V

K

K

P

P

G
|
G

P
|
P

H
|
H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

I

I

P

P

E

K

N

N

L

L

D

D

L

L

S

S

L

I

V

I

D

D

Q

R

V

V

E

E

R

T

V

V

S

D

N

S

E

D

E

T

A

A

I

L

A

A

Y

T

A

A

R

R

L

L

A

M

Q

A

E

E

G

G

L

I

I

L

S

A

G

G

I

I

S

S

C

S

G

G

A

A

V

V

A

A

V

D

R

R

L

L

A

A

Q

K

Q

L

S

P

E

E

H

F

A

A

G

D

K

K

T

L

I

I

V

V

V

I

L

L

P

P

D

S

S

A

G

S

E

E

R

R

Y

Y

L

L

S

S

T

T

A

A

L

L

F

F

binding o-acetylserine: K115 (= K124), G116 (= G125), M117 (= M126)
binding : K41 (= K48), T68 (= T75), G69 (= G77), T71 (= T79), P91 (= P99), M117 (= M126), F141 (= F150), G219 (= G228), P220 (= P229), H221 (= H230), G225 (= G234), A228 (= A237)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
60% identity, 97% coverage: 5:323/329 of query aligns to 72:384/392 of P47999

query
sites
P47999

G

G

T

L

F

N

F

I

A

A

D

D

N

N

S

A

-

A

Q

Q

T

L

I

I

G

G

K

K

T

T

P

P

L

M

V

V

R

Y

L

L

N

N

R

N

I

V

V

V

K

K

G

G

A

C

P

V

A

A

T

S

V

V

L

A

A

A

K

K

I

L

E

E

G

I

R

M

N

E

P

P

A

C

Y

C

S

S

V

V

K

K

C

D

R

R

I

I

G

G

A

Y

A

S

M

M

I

I

W

T

D

D

A

A

E

E

Q

E

R

K

G

G

L

L

L

I

G

T

P

P

G

G

K

K

E

S

-

V

L

L

I

V

E

E

P

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

F

F

V

I

A

A

A

S

R

K

G

G

I

Y

P

K

L

L

T

I

L

L

T

T

M

M

P

P

E

A

T

S

M

M

S

S

L

L

E

E

R

R

K

V

L

L

A

R

A

A

Y

F

G

G

A

A

K

E

L

L

V

V

L

L

T

T

E

E

G

P

V

A

K

K

G

G

M

M

T

T

G

G

A

A

V

I

R

Q

R

K

A

A

E

E

D

E

I

I

A

L

A

K

S

K

D

T

P

P

D

N

R

S

Y

Y

V

-

L

M

L

L

Q

Q

F

F

R

D

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

E

Y

Q

E

T

T

G

G

P

P

E

E

I

I

W

W

E

E

D

D

T

T

G

R

G

G

A

K

I

I

D

D

I

I

L

L

V

V

S

A

G

G

V

I

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

S

G

R

R

Y

F

I

I

K

K

Q

E

T

R

Q

-

G

-

K

K

P

P

I

E

L

L

S

K

V

V

-

I

A

G

V

V

E

E

P

P

E

T

A

E

S

S

P

A

V

I

I

L

S

S

Q

G

R

-

S

-

G

-

L

-

P

-

L

-

K

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

V

P

P

E

K

N

N

L

L

D

D

L

L

S

A

L

I

V

V

D

D

Q

E

V

Y

E

I

R

A

V

I

S

S

N

S

E

E

A

A

I

I

A

E

Y

T

A

S

R

K

R

Q

L

L

A

A

Q

L

E

Q

E

E

G

G

L

L

I

L

S

V

G

G

I

I

S

S

C

S

G

G

A

A

V

A

A

A

V

I

R

Q

L

V

A

A

Q

K

Q

R

S

P

E

E

H

N

A

A

G

G

K

K

T

L

I

I

V

A

V

V

L

F

P

P

D

S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

Q

L

L

F

F

D

Q

G

S

I

I

F

R

N

E

E

E

Sites not aligning to the query:

61 modified: N-acetylalanine

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
59% identity, 97% coverage: 4:323/329 of query aligns to 9:322/323 of 4aecA

query
sites
4aecA

S

D

G

G

T

L

F

N

F

I

A

A

D

D

N

N

-

V

S

S

Q

Q

T

L

I

I

G

G

K

K

T

T

P

P

L

M

V

V

R

Y

L

L

N

N

R

S

I

I

V

A

K

K

G

G

A

C

P

V

A

A

T

N

V

I

L

A

A

A

K

K

I

L

E

E

G

I

R

M

N

E

P

P

A

C

Y

C

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

Y

A

S

M

M

I

V

W

T

D

D

A

A

E

E

Q

Q

R

K

G

G

L

F

L

I

G

S

P

P

G

G

K

K

E

S

-

V

L

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

G

L

L

A

A

F

F

V

I

A

A

A

S

R

R

G

G

I

Y

P

R

L

L

T

I

L

L

T

T

M

M

P

P

E

A

T

S

M

M

S

S

L

M

E

E

R

R

K

V

L

L

A

K

A

A

Y

F

G

G

A

A

K

E

L

L

V

V

L

L

T

T

E

D

G

P

V

A

K

K

G

G

M

M

T

T

G

G

A

A

V

V

R

Q

R

K

A

A

E

E

D

E

I

I

A

L

A

K

S

N

D

T

P

P

D

D

R

A

Y

Y

V

-

L

M

L

L

Q

Q

F

F

R

D

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

E

Y

Q

E

T

T

G

G

P

P

E

E

I

I

W

W

E

D

D

D

T

T

G

K

G

G

A

K

I

V

D

D

I

I

L

F

V

V

S

A

G

G

V
x
I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

V

S

G

R

R

Y

F

I

I

K

K

Q

E

T

K

Q

N

G

P

K

K

P

T

I

Q

L

V

S

-

V

I

A

G

V

V

E

E

P

P

E

T

A

E

S

S

P

D

V

I

I

L

S

S

Q

G

R

-

S

-

G

-

L

-

P

-

L

-

K

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

K

N

N

L

L

D

D

L

Q

S

K

L

I

V

M

D

D

Q

E

V

V

E

I

R

A

V

I

S

S

N

S

E

E

A

A

I

I

A

E

Y

T

A

A

R

K

R

Q

L

L

A

A

Q

L

E

K

E

E

G

G

L

L

I

M

S

V

G

G

I

I

S
|
S

C

S

G

G

A

A

V

A

A

A

V

I

R

K

L

V

A

A

Q

K

Q

R

S

P

E

E

H

N

A

A

G

G

K

K

T

L

I

I

V

A

V

V

L

F

P
|
P

D

S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

P

L

L

F

F

D

Q

G

S

I

I

F

R

N

E

E

E

active site: K54 (= K48), S277 (= S278)
binding pyridoxal-5'-phosphate: K54 (= K48), N85 (= N78), I188 (≠ V182), G189 (= G183), T190 (= T184), G191 (= G185), G192 (= G186), T193 (= T187), G233 (= G234), S277 (= S278), P304 (= P305)

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
60% identity, 94% coverage: 10:317/329 of query aligns to 6:305/306 of 2q3dA

query
sites
2q3dA

D

D

N

I

S

T

Q

Q

T

L

I

I

G

G

K

R

T

T

P

P

L

L

V

V

R

R

L

L

N

R

R

R

I

V

V

T

K

D

G

G

A

A

P

V

A

A

T

D

V

I

L

V

A

A

K

K

I

L

E

E

G

F

R

F

N

N

P

P

A

A

Y

N

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

V

A

A

M

M

I

L

W

Q

D

A

A

A

E

E

Q

Q

R

A

G

G

L

L

L

I

G

K

P

P

G

D

K

T

E

I

L

I

I

L

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

M

V

V

A

C

A

A

R

R

G

G

I

Y

P

R

L

C

T

V

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

L

L

E

E

R

R

K

M

L

L

A

R

A

A

Y

Y

G

G

A

A

K

E

L

L

V

I

L

L

T

T

E

P

G

G

V

A

K

D

G

G

M

M

T

S

G

G

A

A

V

I

R

A

R

K

A

A

E

E

D

E

I

L

A

A

A

K

S

T

D

D

P

-

D

Q

R

R

Y

Y

V

F

L

V

L

P

Q

Q

Q
|
Q

F

F

R

E

N

N

P

P

A

A

N

N

P

P

A

A

I

I

H

H

E

R

Q

V

T

T

G

A

P

E

E

E

I

V

W

W

E

R

D

D

T

T

G

D

G

G

A

K

I

V

D

D

I

I

L

V

V

V

S

A

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

A

R

Q

Y

V

I

I

K

K

Q

E

T

R

Q

K

G

P

K

S

P

A

I

R

L

F

S

-

V

V

A

A

V

V

E

E

P

P

E

A

A

A

S

S

P

P

V

V

I

L

S

S

Q

G

R

Q

S

K

G

-

L

-

P

-

L

-

K

-

P

-

G

G

P

P

H

H

K

P

I

I

Q

Q

G
|
G

I
|
I

G

G

A

A

G

G

F

F

I

V

P

P

E

P

N

V

L

L

D

D

L

Q

S

D

L

L

V

V

D

D

Q

E

V

I

E

I

R

T

V

V

S

G

N

N

E

E

E

D

A

A

I

L

A

N

Y

V

A

A

R

R

L

L

A

A

Q

R

E

E

G

G

L

L

I

L

S

V

G

G

I

I

S
|
S

C

S

G

G

A

A

V

T

A

V

A

A

V

L

R

Q

L

V

A

A

Q

R

S

P

E

E

H

N

A

A

G

G

K

K

T

L

I

I

V

V

L

L

P
|
P

D
|
D

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

P

L

L

F

F

active site: K44 (= K48), S266 (= S278), P293 (= P305)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K48), T71 (= T75), S72 (= S76), N74 (= N78), T75 (= T79), Q144 (= Q149), V177 (= V182), G178 (= G183), T179 (= T184), G180 (= G185), T182 (= T187), G222 (= G234), I223 (= I235), S266 (= S278), P293 (= P305), D294 (= D306)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
60% identity, 94% coverage: 10:317/329 of query aligns to 6:305/310 of P9WP55

query
sites
P9WP55

D

D

N

I

S

T

Q

Q

T

L

I

I

G

G

K

R

T

T

P

P

L

L

V

V

R

R

L

L

N

R

R

R

I

V

V

T

K

D

G

G

A

A

P

V

A

A

T

D

V

I

L

V

A

A

K

K

I

L

E

E

G

F

R

F

N

N

P

P

A

A

Y

N

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

V

A

A

M

M

I

L

W

Q

D

A

A

A

E

E

Q

Q

R

A

G

G

L

L

L

I

G

K

P

P

G

D

K

T

E

I

L

I

I

L

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

F

M

V

V

A

C

A

A

R

R

G

G

I

Y

P

R

L

C

T

V

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

L

L

E

E

R

R

K

M

L

L

A

R

A

A

Y

Y

G

G

A

A

K

E

L

L

V

I

L

L

T

T

E

P

G

G

V

A

K

D

G

G

M

M

T

S

G

G

A

A

V

I

R

A

R

K

A

A

E

E

D

E

I

L

A

A

A

K

S

T

D

D

P

-

D

Q

R

R

Y

Y

V

F

L

V

L

P

Q

Q

F

F

R

E

N

N

P

P

A

A

N

N

P

P

A

A

I

I

H

H

E

R

Q

V

T

T

G

A

P

E

E

E

I

V

W

W

E

R

D

D

T

T

G

D

G

G

A

K

I

V

D

D

I

I

L

V

V

V

S

A

G

G

V

V

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

V

S

A

R

Q

Y

V

I

I

K

K

Q

E

T

R

Q

K

G

P

K

S

P

A

I

R

L

F

S

-

V

V

A

A

V

V

E

E

P

P

E

A

A

A

S

S

P

P

V

V

I

L

S

S

Q

G

R

Q

S

K

G

-

L

-

P

-

L

-

K

-

P

-

G

G

P

P

H

H

K

P

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

V

P

P

E

P

N

V

L

L

D

D

L

Q

S

D

L

L

V

V

D

D

Q

E

V

I

E

I

R

T

V

V

S

G

N

N

E

E

E

D

A

A

I

L

A

N

Y

V

A

A

R

R

L

L

A

A

Q

R

E

E

G

G

L

L

I

L

S

V

G

G

I

I

S
|
S

C

S

G

G

A

A

V

T

A

V

A

A

V

L

R

Q

L

V

A

A

Q

R

S

P

E

E

H

N

A

A

G

G

K

K

T

L

I

I

V

V

L

L

P

P

D

D

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

P

L

L

F

F

K44 (= K48) modified: N6-(pyridoxal phosphate)lysine
N74 (= N78) binding
GTGGT 178:182 (= GTGGT 183:187) binding
S266 (= S278) binding

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
60% identity, 92% coverage: 10:312/329 of query aligns to 6:300/300 of 3zeiA

query
sites
3zeiA

D

D

N

I

S

T

Q

Q

T

L

I

I

G

G

K

R

T

T

P

P

L

L

V

V

R

R

L

L

N

R

R

R

I

V

V

T

K

D

G

G

A

A

P

V

A

A

T

D

V

I

L

V

A

A

K

K

I

L

E

E

G

F

R

F

N

N

P

P

A

A

Y

N

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

V

A

A

M

M

I

L

W

Q

D

A

A

A

E

E

Q

Q

R

A

G

G

L

L

L

I

G

K

P

P

G

D

K

T

E

I

L

I

I

L

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

F

M

V

V

A

C

A

A

R

R

G

G

I

Y

P

R

L

C

T

V

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

L

L

E

E

R

R

K

M

L

L

A

R

A

A

Y

Y

G

G

A

A

K

E

L

L

V

I

L

L

T

T

E

P

G

G

V

A

K

D

G

G

M

M

T

S

G

G

A

A

V
x
I

R

A

R

K

A

A

E

E

D

E

I

L

A

A

A

K

S

T

D

D

P

-

D

Q

R

R

Y

Y

V

F

L

V

L

P

Q

Q

Q
|
Q

F
|
F

R

E

N

N

P

P

A

A

N

N

P

P

A

A

I

I

H

H

E

R

Q

V

T

T

G

A

P

E

E

E

I

V

W

W

E

R

D

D

T

T

G

D

G

G

A

K

I

V

D

D

I

I

L

V

V

V

S

A

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

A

R

Q

Y

V

I

I

K

K

Q

E

T

R

Q

K

G

P

K

S

P

A

I

R

L

F

S

-

V

V

A

A

V

V

E

E

P

P

E

A

A

A

S

S

P

P

V

V

I

L

S

S

Q

G

R

Q

S
x
K

G

-

L

-

P

-

L

-

K

-

P

-

G

G

P

P

H

H

K

P

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F
|
F

I

V

P

P

E

P

N

V

L

L

D

D

L

Q

S

D

L

L

V

V

D

D

Q

E

V

I

E

I

R

T

V

V

S

G

N

N

E

E

E

D

A

A

I

L

A

N

Y

V

A

A

R

R

L

L

A

A

Q

R

E

E

G

G

L

L

I

L

S

V

G

G

I

I

S
|
S

C

S

G

G

A

A

V

T

A

V

A

A

V

L

R

Q

L

V

A

A

Q

R

S

P

E

E

H

N

A

A

G

G

K

K

T

L

I

I

V

V

L

L

P
|
P

D
|
D

S

F

G

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K48), S266 (= S278), P293 (= P305)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T75), S72 (= S76), I126 (≠ V130), Q144 (= Q149), F145 (= F150), K215 (≠ S221), G222 (= G234), A225 (= A237), F227 (= F239)
binding pyridoxal-5'-phosphate: K44 (= K48), N74 (= N78), V177 (= V182), G178 (= G183), T179 (= T184), G180 (= G185), T182 (= T187), G222 (= G234), S266 (= S278), P293 (= P305), D294 (= D306)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
60% identity, 92% coverage: 10:312/329 of query aligns to 6:300/300 of 2q3cA

query
sites
2q3cA

D

D

N

I

S

T

Q

Q

T

L

I

I

G

G

K

R

T

T

P

P

L

L

V

V

R

R

L

L

N

R

R

R

I

V

V

T

K

D

G

G

A

A

P

V

A

A

T

D

V

I

L

V

A

A

K

K

I

L

E

E

G

F

R

F

N

N

P

P

A

A

Y

N

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

V

A

A

M

M

I

L

W

Q

D

A

A

A

E

E

Q

Q

R

A

G

G

L

L

L

I

G

K

P

P

G

D

K

T

E

I

L

I

I

L

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

M

V

V

A

C

A

A

R

R

G

G

I

Y

P

R

L

C

T

V

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

L

L

E

E

R

R

K

M

L

L

A

R

A

A

Y

Y

G

G

A

A

K

E

L

L

V

I

L

L

T

T

E

P

G

G

V

A

K

D

G

G

M
|
M

T

S

G

G

A

A

V

I

R

A

R

K

A

A

E

E

D

E

I

L

A

A

A

K

S

T

D

D

P

-

D

Q

R

R

Y

Y

V

F

L

V

L

P

Q

Q

Q
|
Q

F

F

R

E

N

N

P

P

A

A

N

N

P

P

A

A

I

I

H

H

E

R

Q

V

T

T

G

A

P

E

E

E

I

V

W

W

E

R

D

D

T

T

G

D

G

G

A

K

I

V

D

D

I

I

L

V

V

V

S

A

G

G

V

V

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

S

A

R

Q

Y

V

I

I

K

K

Q

E

T

R

Q

K

G

P

K

S

P

A

I

R

L

F

S

-

V

V

A

A

V

V

E

E

P

P

E

A

A

A

S

S

P

P

V

V

I

L

S

S

Q

G

R

Q

S
x
K

G

-

L

-

P

-

L

-

K

-

P

-

G

G

P

P

H

H

K

P

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

I

V

P

P

E

P

N

V

L

L

D

D

L

Q

S

D

L

L

V

V

D

D

Q

E

V

I

E

I

R

T

V

V

S

G

N

N

E

E

E

D

A

A

I

L

A

N

Y

V

A

A

R

R

L

L

A

A

Q

R

E

E

G

G

L

L

I

L

S

V

G

G

I

I

S
|
S

C

S

G

G

A

A

V

T

A

V

A

A

V

L

R

Q

L

V

A

A

Q

R

S

P

E

E

H

N

A

A

G

G

K

K

T

L

I

I

V

V

L

L

P
|
P

D

D

S

F

G

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K48), S266 (= S278), P293 (= P305)
binding : T71 (= T75), S72 (= S76), G73 (= G77), T75 (= T79), M122 (= M126), Q144 (= Q149), K215 (≠ S221), G222 (= G234), A225 (= A237)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
59% identity, 95% coverage: 10:320/329 of query aligns to 6:311/318 of 4lmaA

query
sites
4lmaA

D

D

N

V

S

T

Q

E

T

L

I

V

G

G

K

R

T

T

P

P

L

L

V

V

R

Q

L

L

N

N

R

R

I

I

-

P

-

Q

V

A

K

E

G

G

A

C

P

L

A

G

T

R

V

I

L

V

A

M

K

K

I

L

E

E

G

G

R

M

N

N

P

P

A

A

Y

A

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

T

A

H

M

M

I

I

W

N

D

S

A

A

E

E

Q

K

R

A

G

G

L

L

L

I

G

N

P

P

G

E

K

T

E

T

-

V

L

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

F

M

V

T

A

A

R

K

G

G

I

Y

P

R

L

L

T

I

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

L

L

E

E

R

R

K

A

L

M

L

L

A

K

A

A

Y

Y

G

G

A

A

K

T

L

L

V

E

L

L

T

T

E

P

G

G

V

S

K

Q

G

G

M

M

T

K

G

G

A

A

V

I

R

L

R

R

A

A

E

Q

D

Q

I

I

A

V

A

D

S

S

D

I

P

P

D

G

R

A

Y

Y

V

-

L

M

L

L

Q

Q

F

F

R

R

N

N

P

P

A

S

N

N

P

P

A

E

I

I

H

H

E

R

Q

L

T

T

G

A

P

E

E

E

I

I

W

W

E

Q

D

D

T

T

G

E

G

G

A

Q

I

V

D

D

I

F

L

I

V

V

S

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

A

R

E

Y

V

I

I

K

K

Q

S

T

R

Q

-

G

-

K

K

P

P

I

S

L

F

S

Q

V

V

-

V

A

A

V

V

E

E

P

P

E

F

A

N

S

S

P

P

V

V

I

I

S

S

Q

G

R

-

S

-

G

-

L

-

P

-

L

-

K

N

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

E

N

V

L

L

D

R

L

T

S

D

L

L

V

I

D

D

Q

E

V

V

E

I

R

T

V

V

S

S

N

D

E

E

A

A

I

F

A

Q

Y

F

A

G

R

R

L

L

A

A

Q

K

E

E

G

G

L

L

I

L

S

S

G

G

I

I

S
|
S

C

S

G

G

A

A

A

N

V

L

A

C

A

A

V

I

R

Q

L

L

A

A

Q

Q

Q

R

S

P

E

E

H

N

A

E

G

G

K

K

T

L

I

I

V

V

V

I

L

Q

P
|
P

D

S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

L

L

M

F

F

D

Q

G

N

I

I

active site: K46 (= K48), S269 (= S278)
binding pyridoxal-5'-phosphate: K46 (= K48), N77 (= N78), G181 (= G183), T182 (= T184), G183 (= G185), T185 (= T187), G225 (= G234), S269 (= S278), P296 (= P305)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
58% identity, 96% coverage: 6:320/329 of query aligns to 2:309/309 of 7n2tA

query
sites
7n2tA

T

T

F

I

F

A

A

K

D

D

N

V

S

T

Q

E

T

L

I

I

G

G

K

N

T

T

P

P

L

L

V

V

R

Y

L

L

N

N

R

R

I

V

V

V

K

D

G

G

A

C

P

V

A

A

T

R

V

V

L

A

A

A

K

K

I

L

E

E

G

M

R

M

N

E

P

P

A

C

Y

S

S

S

V

V

K

K

C

D

R

R

I

I

G

G

A

Y

A

S

M

M

I

I

W

S

D

D

A

A

E

E

Q

N

R

K

G

G

L

L

L

I

G

T

P

P

G

G

K

E

E

S

-

V

L

L

I

I

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

F

F

V

I

A

A

R

K

G

G

I

Y

P

R

L

L

T

I

L

I

T

C

M

M

P

P

E

A

T

S

M

M

S

S

L

L

E

E

R

R

K

T

L

I

L

L

A

R

A

A

Y

F

G

G

A

A

K

E

L

L

V

V

L

L

T

T

E

D

G

P

V

A

K

R

G

G

M

M

T

K

G

G

A

A

V

V

R

Q

R

K

A

A

E

E

D

E

I

I

A

K

A

A

S

K

D

T

P

P

D

N

R

S

Y

Y

V

I

L

L

L

-

Q

Q

F

F

R

E

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

E

Y

Q

E

T

T

G

G

P

P

E

E

I

I

W

W

E

R

D

G

T

S

G

G

A

K

I

I

D

D

I

A

L

L

V

V

S

S

G

G

V

I

G

G

T

T

G

G

T

T

I

V

T

T

G

G

V

A

S

G

R

K

Y

Y

I

L

K

K

Q

E

T

-

Q

Q

G

N

K

P

P

N

I

I

L

K

S

L

V

Y

A

G

V

V

E

E

P

P

E

V

A

E

S

S

P

A

V

I

I

L

S

S

Q

G

R

-

S

-

G

-

L

-

P

-

L

-

K

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

G

N

V

L

L

D

D

L

V

S

N

L

L

V

L

D

D

Q

E

V

V

E

I

R

Q

V

V

S

S

N

S

E

E

A

S

I

I

A

E

Y

T

A

A

R

K

R

L

L

L

A

A

Q

L

E

K

E

E

G

G

L

L

I

L

S

V

G

G

I

I

S

S

C

S

G

G

A

A

V

A

A

A

V

I

R
|
R

L

I

A

A

Q

K

Q
x
R

S

P

E

E

H

N

A

A

G

G

K

K

T

L

I

I

V

V

V

A

V

V

L

F

P

P

D

S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

V

L

L

F

F

D

E

G

S

I

V

binding phosphate ion: R277 (= R288), R281 (≠ Q292)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
56% identity, 95% coverage: 10:323/329 of query aligns to 8:314/322 of P47998

query
sites
P47998

D

D

N

V

S

T

Q

E

T

L

I

I

G

G

K

N

T

T

P

P

L

L

V

V

R

Y

L

L

N

N

R

N

I

V

V

A

K

E

G

G

A

C

P

V

A

G

T

R

V

V

L

A

A

A

K

K

I

L

E

E

G

M

R

M

N

E

P

P

A

C

Y

S

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

F

A

S

M

M

I

I

W

S

D

D

A

A

E

E

Q

K

R

K

G

G

L

L

L

I

G

K

P

P

G

G

K

E

E

S

-

V

L

L

I

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

F

F

V

T

A

A

R

K

G

G

I

Y

P

K

L

L

T

I

L

I

T

T

M

M

P

P

E

A

T

S

M

M

S

S

L

T

E

E

R

R

K

I

L

I

L

L

A

L

A

A

Y

F

G

G

A

V

K

E

L

L

V

V

L

L

T

T

E

D

G

P

V

A

K

K

G

G

M

M

T

K

G

G

A

A

V

I

R

A

R

K

A

A

E

E

D

E

I

I

A

L

A

A

S

K

D

T

P

P

D

N

R

G

Y

Y

V

-

L

M

L

L

Q

Q

Q
|
Q

F

F

R

E

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H
|
H

E

Y

Q

E

T

T

G
|
G

P

P

E

E

I

I

W

W

E

K

D

G

T

T

G

G

A

K

I

I

D

D

I

G

L

F

V

V

S

S

G

G

V

I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

A

S

G

R

K

Y

Y

I

L

K

K

Q

E

T

-

Q

Q

G

N

K

A

P

N

I

V

L

K

S

L

V

Y

A

G

V

V

E

E

P

P

E

V

A

E

S

S

P

A

V

I

I

L

S

S

Q

G

R

-

S

-

G

-

L

-

P

-

L

-

K
|
K

P

P

G

G

P

P

H
|
H

K
|
K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

I

I

P

P

E

S

N

V

L

L

D

N

L

V

S

D

L

L

V

I

D

D

Q

E

V

V

E

V

R

Q

V

V

S

S

N

S

E

D

E

E

A

S

I

I

A

D

Y

M

A

A

R

R

R

Q

L

L

A

A

Q

L

E

K

E

E

G

G

L

L

I

L

S

V

G

G

I

I

S
|
S

C

S

G

G

A

A

V

A

A

A

V

I

R

K

L

L

A

A

Q

Q

Q

R

S

P

E

E

H

N

A

A

G

G

K

K

T

L

I

F

V

V

V

A

V

I

L

F

P

P

D

S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

V

L

L

F

F

D

D

G

A

I

T

F

R

N

K

E

E

K46 (= K48) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T75) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S76) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N78) binding ; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T79) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q149) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H159) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G164) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (= GTGGT 183:187) binding
T182 (= T184) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T187) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (= K226) mutation to A: Impaired interaction with SAT1.
H221 (= H230) mutation to A: Impaired interaction with SAT1.
K222 (= K231) mutation to A: Impaired interaction with SAT1.
S269 (= S278) binding ; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
58% identity, 94% coverage: 10:317/329 of query aligns to 6:308/310 of 4lmbA

query
sites
4lmbA

D

D

N

I

S

T

Q

Q

T

L

I

V

G

G

K

R

T

T

P

P

L

L

V

V

R

Q

L

L

N

N

R

R

I

I

-

P

-

V

V

A

K

E

G

G

A

V

P

K

A

A

T

R

V

I

L

V

A

V

K

K

I

L

E

E

G

S

R

M

N

N

P

P

A

A

Y

A

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

V

A

S

M

M

I

V

W

E

D

D

A

A

E

E

Q

A

R

A

G

G

L

L

L

I

G

H

P

P

G

D

K

K

E

T

-

I

L

L

I

V

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

M

V

V

A

A

R

K

G

G

I

Y

P

R

L

L

T

V

L

L

T

T

M

M

P

P

E

E

T

T

M
|
M

S

S

L

L

E

E

R

R

K

A

L

M

L

L

A

K

A

A

Y

Y

G

G

A

A

K

Q

L

L

V

E

L

L

T

T

E

P

G

G

V

S

K

Q

G

G

M
|
M

T

E

G

G

A

A

V

I

R

T

R

R

A

A

E

E

D

E

I

I

A

V

A

E

S

N

D

T

P

P

D

N

R

A

Y

Y

V

S

L

L

L

-

Q

Q

Q
|
Q

F
|
F

R

R

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

E

R

Q

E

T

T

G

A

P

E

E

E

I

I

W

W

E

A

D

D

T

T

G

D

G

G

A

L

I

V

D

D

I

I

L

V

V

I

S

G

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

I

S

A

R

E

Y

T

I

I

K

K

Q

P

T

R

Q

R

G

P

K

Q

P

-

I

F

L

Q

S

A

V

I

A

A

V

V

E

E

P

P

E

S

A

N

S

S

P

P

V

V

I

L

S

S

Q

G

R

-

S

-

G

-

L

-

P

-

L

-

K

Q

P

P

G

G

P

P

H

H

K

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

I

I

P

P

E

A

N

I

L

F

D

R

L

P

S

E

L

L

V

I

D

D

Q

E

V

V

E

I

R

I

V

V

S

D

N

D

E

T

E

E

A

A

I

F

A

A

Y

Y

A

A

R

R

L

L

A

A

Q

R

E

Q

E

E

G

G

L

L

I

L

S

S

G

G

I

I

S
|
S

C

A

G

G

A

A

V

L

A

W

A

A

V

I

R

Q

L

V

A

G

Q

K

Q

R

S

P

E

E

H

N

A

E

G

D

K

K

T

L

I

I

V

V

V

M

V

I

L

Q

P
|
P

D

S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

A

A

L

L

F

F

active site: K46 (= K48), S269 (= S278)
binding cysteine: K46 (= K48), T74 (= T75), S75 (= S76), N77 (= N78), T78 (= T79), M101 (= M102), M125 (= M126), M125 (= M126), Q147 (= Q149), F148 (= F150), Q224 (= Q233), G225 (= G234), G225 (= G234), I226 (= I235), A228 (= A237)
binding pyridoxal-5'-phosphate: K46 (= K48), N77 (= N78), V180 (= V182), G181 (= G183), T182 (= T184), G183 (= G185), T185 (= T187), G225 (= G234), S269 (= S278), P296 (= P305)

Query Sequence

>Synpcc7942_B2664 FitnessBrowser__SynE:Synpcc7942_B2664
MSTSGTFFADNSQTIGKTPLVRLNRIVKGAPATVLAKIEGRNPAYSVKCRIGAAMIWDAE
QRGLLGPGKELIEPTSGNTGIALAFVAAARGIPLTLTMPETMSLERRKLLAAYGAKLVLT
EGVKGMTGAVRRAEDIAASDPDRYVLLQQFRNPANPAIHEQTTGPEIWEDTGGAIDILVS
GVGTGGTITGVSRYIKQTQGKPILSVAVEPEASPVISQQRSGLPLKPGPHKIQGIGAGFI
PENLDLSLVDQVERVSNEEAIAYARRLAQEEGLISGISCGAAVAAAVRLAQQSEHAGKTI
VVVLPDSGERYLSTALFDGIFNEQGLAVV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory