SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing TX73_016180 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 11 hits to proteins with known functional sites (download)

P0A7N9 Large ribosomal subunit protein bL33; 50S ribosomal protein L33 from Escherichia coli (strain K12) (see paper)
60% identity, 100% coverage: 1:55/55 of query aligns to 1:55/55 of P0A7N9

query
sites
P0A7N9

M
|
M

A
|
A

K

K

A

G

V

I

T

R

I

E

K

K

I

I

K

K

L

L

V

V

S

S

T

S

A

A

D

G

T

T

G

G

F

H

Y

F

Y

Y

V

T

T

T

K

T

K

K

N

N

S

K

R

R

T

T

M

K

T

P

D

E

K

K

M

L

V

E

K

L

K

K

K

K

Y

F

D

D

P

P

V

V

A

V

R

R

K

Q

H

H

V

V

E

I

F

Y

K

K

E

E

A

A

K

K

I

I

K

K

M1 (= M1) modified: Initiator methionine, Removed
A2 (= A2) modified: N-methylalanine

8rd8bk Small ribosomal subunit protein uS5 (see paper)
70% identity, 85% coverage: 8:54/55 of query aligns to 3:49/49 of 8rd8bk

query
sites
8rd8bk

K
|
K

I

I

K

K

L

L

V

V

S

S

T

T

A

A

D

G

T

T

G
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G

F

Y

Y
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Y

Y
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Y

V

T

T
|
T

K
x
T

K
|
K

N
|
N

S

K

R

R

T

T

M

M

T

P

D

G

K

K

M

M

V

E

K
x
M

K
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K

K

K

Y
x
F

D

D

P
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P

V

K

A

I

R
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R

K

Q

H
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H

V

V

E

L

F

F

K

K

E

E

A

A

K

K

I

I

binding : K3 (= K8), G13 (= G18), Y15 (= Y20), Y16 (= Y21), T18 (= T23), T19 (≠ K24), K20 (= K25), N21 (= N26), M31 (≠ K36), K32 (= K37), F34 (≠ Y39), P36 (= P41), R39 (= R44), H41 (= H46)

Sites not aligning to the query:

binding : 1

5aka1 Em structure of ribosome-srp-ftsy complex in closed state (see paper)
59% identity, 98% coverage: 2:55/55 of query aligns to 1:54/54 of 5aka1

query
sites
5aka1

A
|
A

K
|
K

A

G

V

I

T

R

I
x
E

K
|
K

I

I

K

K

L

L

V

V

S

S

T

S

A

A

D

G

T

T

G

G

F
x
H

Y

F

Y
|
Y

V

T

T

T

K

T

K
|
K

N

N

S
x
K

R

R

T

T

M
x
K

T

P

D
x
E

K

K

M
x
L

V
x
E

K

L

K
|
K

K
|
K

Y
x
F

D
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D

P

P

V

V

A
x
V

R
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R

K
x
Q

H
|
H

V

V

E

I

F

Y

K

K

E

E

A

A

K

K

I

I

K

K

binding : A1 (= A2), K2 (= K3), E6 (≠ I7), K7 (= K8), H18 (≠ F19), Y20 (= Y21), K24 (= K25), K26 (≠ S27), K29 (≠ M30), E31 (≠ D32), L33 (≠ M34), E34 (≠ V35), K36 (= K37), K37 (= K38), F38 (≠ Y39), D39 (= D40), V42 (≠ A43), R43 (= R44), Q44 (≠ K45), H45 (= H46)

7m4v0 A. Baumannii ribosome-eravacycline complex: 50s (see paper)
65% identity, 87% coverage: 8:55/55 of query aligns to 4:51/51 of 7m4v0

query
sites
7m4v0

K
|
K

I

I

K

R

L

L

V

V

S

S

T

S

A

A

D

G

T

T

G

G

F

Y

Y
x
F

Y

Y

V

T

T

T

K

T

K

K

N
|
N

S

K

R

R

T

T

M
|
M

T

P

D

E

K

K

M
|
M

V

E

K
x
I

K
|
K

K

K

Y
x
F

D

D

P
|
P

V

K

A

I

R
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R

K

Q

H
|
H

V

V

E

I

F

F

K

K

E

E

A

A

K

K

I

I

K

K

binding : K4 (= K8), F16 (≠ Y20), N22 (= N26), M26 (= M30), M30 (= M34), I32 (≠ K36), K33 (= K37), F35 (≠ Y39), P37 (= P41), R40 (= R44), H42 (= H46)

Sites not aligning to the query:

binding : 2

7unr5 30S ribosomal protein S6
62% identity, 85% coverage: 9:55/55 of query aligns to 5:51/51 of 7unr5

query
sites
7unr5

I

I

K

R

L

L

V

V

S

S

T

S

A

A

D

G

T

T

G

G

F

H

Y
x
F

Y

Y

V

T

T

T

K

D

K

K

N

N

S

K

R

R

T

T

M
x
K

T

P

D

E

K

K

M

I

V

E

K
x
I

K
|
K

K

K

Y
|
Y

D

D

P

P

V

V

A

V

R
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R

K

Q

H
|
H

V

V

E

I

F

Y

K

K

E

E

A

A

K

K

I
|
I

K

K

binding : F16 (≠ Y20), K26 (≠ M30), I32 (≠ K36), K33 (= K37), Y35 (= Y39), R40 (= R44), H42 (= H46), I50 (= I54)

Sites not aligning to the query:

binding : 1, 2

4io91 Crystal structure of compound 4d bound to large ribosomal subunit (50s) from deinococcus radiodurans (see paper)
57% identity, 85% coverage: 7:53/55 of query aligns to 7:53/53 of 4io91

query
sites
4io91

I

I

K

I

I

V

K

K

L

M

V

E

S

S

T

S

A

A

D

G

T

T

G

G

F

F

Y

Y

Y

Y

V

T

T

T

K

T

K

K

N

N

S

R

R

R

T

N

M

T

T

Q

D

A

K

K

M

L

V

E

K

L

K

K

K
|
K

Y

Y

D

D

P

P

V

V

A

A

R

K

K

K

H

H

V

V

E

V

F

F

K

R

E

E

A

K

K

K

binding : K38 (= K38)

Sites not aligning to the query:

binding : 1, 2

7pktz mL119 (see paper)
53% identity, 93% coverage: 3:53/55 of query aligns to 3:53/53 of 7pktz

query
sites
7pktz

K

K

A

G

V

V

T
x
R

I

L

K

L

I

V

K

K

L

L

V

V

S

S

T

T

A

A

D

K

T
|
T

G
|
G

F

F

Y
x
F

Y
|
Y

V

V

T
|
T

K
x
E

K

K

N
|
N

S

P

R

R

T
x
N

M

T

T

P

D

W

K

K

M

L

V

K

K
x
L

K
x
M

K
|
K

Y
x
F

D

D

P
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P

V

K

A

V

R

N

K

K

H
|
H

V

V

E

L

F

F

K

E

E

E

A

Q

K

K

binding : R6 (≠ T6), T17 (= T17), G18 (= G18), F20 (≠ Y20), Y21 (= Y21), T23 (= T23), E24 (≠ K24), N26 (= N26), N29 (≠ T29), L36 (≠ K36), M37 (≠ K37), K38 (= K38), F39 (≠ Y39), P41 (= P41), H46 (= H46)

6xywAC At4g35490 (see paper)
47% identity, 85% coverage: 9:55/55 of query aligns to 5:51/51 of 6xywAC

query
sites
6xywAC

I

I

K

R

L

L

V

V

S

S

T

A

A

A

D

G

T

T

G
|
G

F

F

Y
x
F

Y
|
Y

V

V

T
x
K

K
x
R

K

K

N
x
S

S

T

R

K

T

G

M

L

T

L

D

E

K

K

M
x
L

V

E

K
x
F

K
x
R

K
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K

Y
|
Y

D

D

P
|
P

V

R

A

V

R
x
N

K
x
R

H
|
H

V

V

E

L

F

F

K

T

E

E

A

Q

K

K

I

M

K
|
K

binding : G14 (= G18), F16 (≠ Y20), Y17 (= Y21), K19 (≠ T23), R20 (≠ K24), S22 (≠ N26), L30 (≠ M34), F32 (≠ K36), R33 (≠ K37), K34 (= K38), Y35 (= Y39), P37 (= P41), N40 (≠ R44), R41 (≠ K45), H42 (= H46), K51 (= K55)

8a22AA uS3m-2 (fragment) (see paper)
49% identity, 85% coverage: 9:55/55 of query aligns to 4:50/50 of 8a22AA

query
sites
8a22AA

I

V

K

K

L

M

V

V

S

S

T

L

A

A

D

K

T

T

G
|
G

F

Y

Y

F

Y

Y

V

V

T

T

K

T

K

K

N
|
N

S

P

R

R

T
x
N

M
x
T

T

P

D

W

K

K

M
x
L

V

K

K

L

K
x
M

K

K

Y
x
F

D

D

P

P

V

V

A

V

R

G

K
x
R

H
|
H

V

V

E

L

F

F

K

E

E

E

A

S

K

K

I

L

K

K

binding : G13 (= G18), N21 (= N26), N24 (≠ T29), T25 (≠ M30), L29 (≠ M34), M32 (≠ K37), F34 (≠ Y39), R40 (≠ K45), H41 (= H46)

Sites not aligning to the query:

binding : 1

6z1pAE mS93 (see paper)
42% identity, 89% coverage: 6:54/55 of query aligns to 3:52/57 of 6z1pAE

query
sites
6z1pAE

T

S

I

I

K

L

I

F

K

K

L

L

V

V

S

S

T

S

A

A

D

G

T
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T

G
|
G

F

F

Y
x
F

Y
|
Y

V

L

T

A

K
|
K

K

R

N

N

S

A

R

K

T

A

-

E

M

V

T

I

D

K

K

K

M

L

V

S

K
x
L

K
x
R

K
|
K

Y
x
F

D

D

P
|
P

V
x
I

A

I

R
x
N

K

Q

H
x
Y

V

V

E

V

F

F

K

N

E

E

A

A

K

K

I

L

binding : T14 (= T17), G15 (= G18), F17 (≠ Y20), Y18 (= Y21), K21 (= K24), L34 (≠ K36), R35 (≠ K37), K36 (= K38), F37 (≠ Y39), P39 (= P41), I40 (≠ V42), N42 (≠ R44), Y44 (≠ H46)

Sites not aligning to the query:

binding : 1

5dm61 Crystal structure of the 50s ribosomal subunit from deinococcus radiodurans (see paper)
53% identity, 69% coverage: 4:41/55 of query aligns to 6:43/54 of 5dm61

query
sites
5dm61

A

A

V
x
P

T
x
R

I

I

K

I

I

V

K

K

L

M

V

E

S

S

T

S

A

A

D

G

T

T

G
|
G

F

F

Y
|
Y

Y

Y

V

T

T

T

K

T

K

K

N
|
N

S

R

R

R

T
x
N

M

T

T

Q

D

A

K

K

M

L

V

E

K
x
L

K
|
K

K
|
K

Y
|
Y

D

D

P

P

binding : P7 (≠ V5), R8 (≠ T6), G20 (= G18), Y22 (= Y20), N28 (= N26), N31 (≠ T29), L38 (≠ K36), K39 (= K37), K40 (= K38), Y41 (= Y39)

Sites not aligning to the query:

binding : 1, 46, 47

Query Sequence

>TX73_016180
MAKAVTIKIKLVSTADTGFYYVTKKNSRTMTDKMVKKKYDPVARKHVEFKEAKIK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory