SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_002719401.1 NCBI__GCF_000015985.1:WP_002719401.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

6j2lA Crystal structure of bi-functional enzyme (see paper)
45% identity, 64% coverage: 3:68/103 of query aligns to 113:178/200 of 6j2lA

query
sites
6j2lA

V

F

L

L

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Y

R

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L

L

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D

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binding zinc ion: E152 (= E42), E171 (= E61), D174 (= D64)

Sites not aligning to the query:

binding zinc ion: 79, 81, 83, 96, 103

7bgnA Crystal structure of mthisn2-amp complex, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
43% identity, 87% coverage: 1:90/103 of query aligns to 115:203/204 of 7bgnA

query
sites
7bgnA

M

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binding adenosine monophosphate: L121 (= L7), R128 (= R14), S135 (= S22), W136 (= W23), T137 (= T24), Y180 (= Y67), R203 (= R90)
binding zinc ion: E154 (= E42), E174 (= E61), D177 (= D64)

Sites not aligning to the query:

binding adenosine monophosphate: 60, 62, 63, 65, 66, 94, 96, 204
binding zinc ion: 78, 80, 82, 95, 102

7bgmA Crystal structure of mthisn2, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
42% identity, 87% coverage: 1:90/103 of query aligns to 125:212/213 of 7bgmA

query
sites
7bgmA

M

L

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T

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binding zinc ion: E163 (= E42), E183 (= E61), D186 (= D64)

Sites not aligning to the query:

binding zinc ion: 80, 82, 84, 97, 104

6j2lB Crystal structure of bi-functional enzyme (see paper)
45% identity, 43% coverage: 25:68/103 of query aligns to 120:163/185 of 6j2lB

query
sites
6j2lB

A

A

K

D

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L

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binding magnesium ion: E137 (= E42), E140 (= E45), E156 (= E61), D159 (= D64)

Sites not aligning to the query:

binding zinc ion: 78, 80, 82

Query Sequence

>WP_002719401.1 NCBI__GCF_000015985.1:WP_002719401.1
MTVLERLAATVEARKGADPDSSWTAKLLAKGPEKCAEKFGEEAVEAIIEAVRGDRARLAS
EAADVLYHLLVMLAARDVTLAEVMAVLEAREGTSGIAEKAGRG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory