SitesBLAST
Comparing WP_002879650.1 NCBI__GCF_000387565.1:WP_002879650.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4g5dA X-ray crystal structure of prostaglandin f synthase from leishmania major friedlin bound to NADPH (see paper)
49% identity, 96% coverage: 12:285/285 of query aligns to 10:283/283 of 4g5dA
- active site: D48 (= D50), Y53 (= Y55), K78 (= K80), H111 (= H113)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G22 (= G24), V23 (≠ T25), W24 (= W26), D48 (= D50), Y53 (= Y55), H111 (= H113), S148 (= S150), N149 (= N151), Q170 (= Q172), W196 (≠ Y198), S197 (= S199), P198 (= P200), L199 (= L201), Q201 (≠ T203), G202 (= G204), L205 (= L207), I237 (≠ L239), P238 (= P240), K239 (= K241), S240 (= S242), V241 (= V243), H242 (= H244), R245 (≠ Y247), E248 (≠ A250), N249 (= N251)
Q9GV41 9,11-endoperoxide prostaglandin H2 reductase; Prostaglandin F2-alpha synthase; EC 1.1.1.- from Trypanosoma brucei brucei
45% identity, 97% coverage: 6:281/285 of query aligns to 3:270/276 of Q9GV41
1vbjA The crystal structure of prostaglandin f synthase from trypanosoma brucei
45% identity, 97% coverage: 6:281/285 of query aligns to 8:275/281 of 1vbjA
- active site: D52 (= D50), Y57 (= Y55), K82 (= K80), H115 (= H113)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G26 (= G24), M27 (≠ T25), W28 (= W26), D52 (= D50), Y57 (= Y55), H115 (= H113), N145 (= N151), Q166 (= Q172), W192 (≠ Y198), S193 (= S199), P194 (= P200), L195 (= L201), Q197 (≠ T203), G198 (= G204), V201 (≠ L207), A218 (= A224), I233 (≠ L239), K235 (= K241), S236 (= S242), G237 (≠ V243), R241 (≠ Y247), E244 (≠ A250), N245 (= N251)
3d3fA Crystal structure of yvgn and cofactor NADPH from bacillus subtilis (see paper)
48% identity, 98% coverage: 6:285/285 of query aligns to 4:275/275 of 3d3fA
- active site: D48 (= D50), Y53 (= Y55), K78 (= K80), H111 (= H113)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G22 (= G24), F24 (≠ W26), D48 (= D50), Y53 (= Y55), H111 (= H113), S140 (= S150), N141 (= N151), Q162 (= Q172), W188 (≠ Y198), S189 (= S199), P190 (= P200), L191 (= L201), Q193 (≠ T203), L197 (= L207), I229 (≠ L239), K231 (= K241), S232 (= S242), K234 (≠ H244), R237 (≠ Y247), E240 (≠ A250), N241 (= N251)
3b3dA B.Subtilis ytbe (see paper)
47% identity, 100% coverage: 1:285/285 of query aligns to 1:280/280 of 3b3dA
A0QV10 Aldo-keto reductase MSMEG_2408/MSMEI_2347; EC 1.1.1.- from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
45% identity, 96% coverage: 12:285/285 of query aligns to 7:271/275 of A0QV10
- K262 (≠ G274) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
4fziA Crystal structure of prostaglandin f synthase from trypanosoma cruzi (see paper)
42% identity, 95% coverage: 12:281/285 of query aligns to 6:270/277 of 4fziA
4gieA Crystal structure of prostaglandin f synthase from trypanosoma cruzi bound to NADP (see paper)
42% identity, 95% coverage: 12:281/285 of query aligns to 17:281/288 of 4gieA
- active site: D55 (= D50), Y60 (= Y55), K85 (= K80), H118 (= H113)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G29 (= G24), W31 (= W26), D55 (= D50), Y60 (= Y55), H118 (= H113), W119 (= W114), N148 (= N151), Q169 (= Q172), W195 (≠ Y198), S196 (= S199), P197 (= P200), L198 (= L201), S200 (≠ T203), L207 (= L207), A224 (= A224), I239 (≠ L239), P240 (= P240), K241 (= K241), S242 (= S242), R247 (≠ Y247), E250 (≠ A250), N251 (= N251)
Q9JII6 Aldo-keto reductase family 1 member A1; Alcohol dehydrogenase [NADP(+)]; Aldehyde reductase; Glucuronate reductase; Glucuronolactone reductase; S-nitroso-CoA reductase; ScorR; EC 1.1.1.2; EC 1.1.1.372; EC 1.1.1.54; EC 1.1.1.19; EC 1.1.1.20; EC 1.6.-.- from Mus musculus (Mouse)
40% identity, 91% coverage: 12:270/285 of query aligns to 8:292/325 of Q9JII6
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylthreonine
P51635 Aldo-keto reductase family 1 member A1; 3-DG-reducing enzyme; Alcohol dehydrogenase [NADP(+)]; Aldehyde reductase; Glucuronate reductase; Glucuronolactone reductase; S-nitroso-CoA reductase; ScorR; EC 1.1.1.2; EC 1.1.1.372; EC 1.1.1.54; EC 1.1.1.19; EC 1.1.1.20; EC 1.6.-.- from Rattus norvegicus (Rat) (see paper)
39% identity, 91% coverage: 12:270/285 of query aligns to 8:292/325 of P51635
- K13 (= K17) Not glycated
- K23 (≠ Q27) modified: carbohydrate, N-linked (Glc) (glycation) lysine
- K30 (≠ Q35) Not glycated
- K34 (≠ E39) Not glycated
- K61 (= K66) Not glycated
- K68 (vs. gap) modified: carbohydrate, N-linked (Glc) (glycation) lysine
- K80 (= K80) Not glycated
- K85 (≠ S85) modified: carbohydrate, N-linked (Glc) (glycation) lysine
- K97 (≠ D97) Not glycated
- K127 (≠ G127) Not glycated
- K134 (vs. gap) Not glycated
- K141 (vs. gap) modified: carbohydrate, N-linked (Glc) (glycation) lysine
- K145 (≠ R133) Not glycated
- K153 (≠ A141) modified: carbohydrate, N-linked (Glc) (glycation) lysine
- K157 (≠ R145) Not glycated
- K240 (= K218) Not glycated
- K257 (≠ N235) Not glycated
- K263 (= K241) Not glycated
- K287 (≠ A265) Not glycated
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylthreonine
- 294 Not glycated
- 308 Not glycated
3cv7A Crystal structure of porcine aldehyde reductase ternary complex (see paper)
39% identity, 91% coverage: 12:270/285 of query aligns to 8:292/322 of 3cv7A
- active site: D45 (= D50), Y50 (= Y55), K80 (= K80), H113 (= H113)
- binding 3,5-dichloro-2-hydroxybenzoic acid: W22 (= W26), Y50 (= Y55), W82 (= W82), H113 (= H113)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G24), T21 (= T25), W22 (= W26), Y50 (= Y55), H113 (= H113), Q184 (= Q172), Y210 (= Y198), S211 (= S199), P212 (= P200), L213 (= L201), S215 (≠ T203), A246 (= A224), I261 (≠ L239), P262 (= P240), K263 (= K241), S264 (= S242), V265 (= V243), T266 (≠ H244), R269 (≠ Y247), Q272 (≠ A250), N273 (= N251)
Sites not aligning to the query:
3h4gA Structure of aldehyde reductase holoenzyme in complex with potent aldose reductase inhibitor fidarestat: implications for inhibitor binding and selectivity (see paper)
39% identity, 91% coverage: 12:270/285 of query aligns to 8:292/320 of 3h4gA
- active site: D45 (= D50), Y50 (= Y55), K80 (= K80), H113 (= H113)
- binding (2s,4s)-2-aminoformyl-6-fluoro-spiro[chroman-4,4'-imidazolidine]-2',5'-dione: W22 (= W26), Y50 (= Y55), H113 (= H113), W114 (= W114), W220 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G24), T21 (= T25), W22 (= W26), D45 (= D50), Y50 (= Y55), H113 (= H113), S162 (= S150), N163 (= N151), Q184 (= Q172), Y210 (= Y198), S211 (= S199), P212 (= P200), L213 (= L201), S215 (≠ T203), D217 (≠ G205), A246 (= A224), I261 (≠ L239), P262 (= P240), K263 (= K241), S264 (= S242), V265 (= V243), T266 (≠ H244), R269 (≠ Y247), Q272 (≠ A250), N273 (= N251)
Sites not aligning to the query:
3fx4A Porcine aldehyde reductase in ternary complex with inhibitor (see paper)
39% identity, 91% coverage: 12:270/285 of query aligns to 8:292/325 of 3fx4A
- active site: D45 (= D50), Y50 (= Y55), K80 (= K80), H113 (= H113)
- binding [(5Z)-5-{[3-(carboxymethoxy)-4-methoxyphenyl]methylidene}-2,4-dioxo-1,3-thiazolidin-3-yl]acetic acid: W22 (= W26), Y50 (= Y55), H113 (= H113), R218 (vs. gap), A219 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G24), T21 (= T25), W22 (= W26), D45 (= D50), Y50 (= Y55), H113 (= H113), Q184 (= Q172), Y210 (= Y198), S211 (= S199), P212 (= P200), L213 (= L201), S215 (≠ T203), A246 (= A224), I261 (≠ L239), P262 (= P240), K263 (= K241), S264 (= S242), V265 (= V243), T266 (≠ H244), R269 (≠ Y247), Q272 (≠ A250), N273 (= N251)
Sites not aligning to the query:
P50578 Aldo-keto reductase family 1 member A1; Alcohol dehydrogenase [NADP(+)]; Aldehyde reductase; Glucuronate reductase; Glucuronolactone reductase; S-nitroso-CoA reductase; ScorR; EC 1.1.1.2; EC 1.1.1.372; EC 1.1.1.54; EC 1.1.1.19; EC 1.1.1.20; EC 1.6.-.- from Sus scrofa (Pig) (see 2 papers)
39% identity, 91% coverage: 12:270/285 of query aligns to 8:292/325 of P50578
- T21 (= T25) binding NADP(+)
- W22 (= W26) binding NADP(+)
- D45 (= D50) binding NADP(+)
- Y50 (= Y55) active site, Proton donor; mutation to F: Abolished aldo-keto reductase activity.
- S162 (= S150) binding NADP(+)
- N163 (= N151) binding NADP(+)
- S211 (= S199) binding NADP(+)
- L213 (= L201) binding NADP(+)
- S215 (≠ T203) binding NADP(+)
- S216 (≠ G204) binding NADP(+)
- K263 (= K241) binding NADP(+)
- S264 (= S242) binding NADP(+)
- V265 (= V243) binding NADP(+)
- T266 (≠ H244) binding NADP(+)
- R269 (≠ Y247) binding NADP(+)
- Q272 (≠ A250) binding NADP(+)
- N273 (= N251) binding NADP(+)
P14550 Aldo-keto reductase family 1 member A1; Alcohol dehydrogenase [NADP(+)]; Aldehyde reductase; Glucuronate reductase; Glucuronolactone reductase; S-nitroso-CoA reductase; ScorR; EC 1.1.1.2; EC 1.1.1.372; EC 1.1.1.54; EC 1.1.1.19; EC 1.1.1.20; EC 1.6.-.- from Homo sapiens (Human) (see 5 papers)
39% identity, 91% coverage: 12:270/285 of query aligns to 8:292/325 of P14550