SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_002964888.1 NCBI__GCF_000022745.1:WP_002964888.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
72% identity, 100% coverage: 1:651/651 of query aligns to 1:646/648 of Q89WV5

query
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Q89WV5

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G263 (= G266) mutation to I: Loss of activity.
G266 (= G269) mutation to I: Great decrease in activity.
K269 (= K272) mutation to G: Great decrease in activity.
E414 (= E417) mutation to Q: Great decrease in activity.

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
66% identity, 97% coverage: 17:648/651 of query aligns to 18:646/652 of P27550

query
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P27550

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K609 (= K611) modified: N6-acetyllysine; by autocatalysis

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
66% identity, 97% coverage: 17:648/651 of query aligns to 18:646/652 of Q8ZKF6

query
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Q8ZKF6

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G

G

R

K

R

I

I

L

D

D

W

W

F

I

K

T

P

P

F

Y

T

Q

K

K

V

V

K

K

N

N

T

T

D

S

F

F

N

A

-

P

G

G

D

N

V

V

T

S

I

I

K

K

W

W

Y

Y

E

E

D

D

G

G

V

T

T

L

N

N

V

L

S

A

Y

A

N

N

C

C

I

L

D

D

R

R

H

H

L

L

K

Q

S

E

R

N

G

G

D

D

K

R

V

T

A

A

I

I

W

W

E

E

G

G

D

D

N

D

P

T

Y

S

I

Q

D

S

K

K

K

H

I

I

T

S

Y

Y

R

R

E

E

L

L

Y

H

E

R

N

D

V

V

C

C

R

R

M

F

A

A

N

N

V

T

L

L

K

L

K

D

H

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

T

A

I

I

Y

Y

L

M

P

P

M

M

I

V

P

P

E

E

A

A

Y

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

S

S

V

V

V

I

F

F

A

G

G

G

F

F

S

S

P

P

E

E

A

A

L

V

A

A

G

G

R

R

I

I

V

I

D

D

C

S

E

S

S

S

T

R

F

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R

R

G

A

G

G

K
x
R

P

S

V

I

A

P

L

L

K

K

E

K

N

N

T

V

D

D

T

D

A

A

I

L

D

K

I

-

A

-

A

N

K

P

Q

N

Y

V

V

T

M

S

V

V

N

E

K

H

V

V

L

I

V

V

V

L

R

K

R

R

T

T

G

G

G

S

K

D

V

I

S

D

W

W

G

Q

R

E

G

G

R

R

D

D

L

L

W

W

Y

W

H

R

Q

D

E

L

V

I

A

E

S

K

V

A

E

S

P

P

H

E

C

H

E

Q

P

P

E

E

P

A

M

M

N

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T

T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

L

L

V

V

Y

Y

A

A

S

A

M

T

T

T

H

F

Q

K

Y

Y

V

V

F

F

D

D

Y

Y

H

H

D

P

G

G

E

D

I

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

V

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

T

T

L

L

M

M

F

F

E

E

G

G

V

V

P

P

N
|
N

F

W

P

P

D

T

Q

P

G

A

R

R

F

M

W

C

E

Q

V

V

D

D

K

K

H

H

H

Q

V

V

N

N

I

I

F

L

Y

Y

T

T

A
|
A

P

P

T

T

A

A

L

I

R

R

A

A

L

L

M

M

G

A

A

E

G

G

D

D

E

K

F

A

V

I

T

E

R

G

S

T

S

D

R

R

S

S

T

S

L

L

R

R

L

I

L

L

G

G

S

S

V

V

G

G

E

E

P

P

I

I

N

N

P

P

E

E

A

A

W

W

E

E

W

W

Y

Y

Y

W

N

K

V

K

V

I

G

G

D

K

Q

E

K

K

C

C

P

P

I

V

V

V

D

D

T

T

W

W

Q

Q

T

T

E

E

N

T

G

G

I

F

L

M

I

I

T

T

P

P

L

L

P

P

G

G

A

A

T

I

D

E

L

L

K

K

P

A

G

G

S

S

A

A

T

T

R

R

P

P

F

F

G

G

V

V

K

Q

P

P

V

A

L

L

V

V

D

D

N

N

E

E

G

G

N

H

V

P

Q

Q

E

E

G

G

V

A

A

T

D

E

G

G

N

N

L

L

C

V

I

I

S

T

D

D

S

S

W

W

P

P

G

G

Q

Q

M

A

R

R

T

T

V

L

Y

F

G

G

D

D

H

H

K

E

R

R

F

F

I

E

E

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

K

K

G

N

M

M

Y

Y

F

F

S

S

G

G

D

D

G

G

C

A

R

R

D

D

E

E

D

D

G

G

Y

Y

W

W

I

I

T

T

G

G

R

R

V

V

D
|
D

D

D

V

V

L

L

N

N

I

V

S
|
S

G
|
G

H

H

R
|
R

L

L

G

G

T

T

A

A

E

E

I

I

E

E

S

S

A

A

L

L

V

V

S

A

H

H

H

P

S

K

V

I

S

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

P

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

C

A

Y

Y

V

V

T

T

L

L

M

N

T

H

G

G

A

E

D

E

A

P

Q

S

D

-

P

-

D

P

E

E

L

L

R

Y

K

A

E

E

L

V

V

R

Q

N

H

W

V

V

R
|
R

K

K

E

E

I

I

G

G

P

P

I

L

A

A

T

T

P

P

D

D

K

V

I

L

Q

H

F

W

A

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

D

G

E

D

F

T

G

S

A

N

L

L

G

G

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

G

G

V

V

D

E

D

K

L

L

I

L

E

E

N

E

R

K

Q

Q

R194 (≠ K192) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T311) binding CoA
N335 (= N335) binding CoA
A357 (= A357) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D517) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (= S523) binding CoA
G524 (= G524) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R526) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (= R586) binding CoA; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K611) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
67% identity, 97% coverage: 17:648/651 of query aligns to 14:639/640 of 5jrhA

query
sites
5jrhA

L

L

I

I

D

N

N

P

E

E

T

Q

Y

Y

L

E

E

T

W

K

Y

Y

E

K

E

Q

S

S

V

I

S

N

D

D

P

P

D

D

G

T

F

F

W

W

A

G

K

E

H

Q

G

G

R

K

R

I

I

L

D

D

W

W

F

I

K

T

P

P

F

Y

T

Q

K

K

V

V

K

K

N

N

T

T

D

S

F

F

N

A

-

P

G

G

D

N

V

V

T

S

I

I

K

K

W

W

Y

Y

E

E

D

D

G

G

V

T

T

L

N

N

V

L

S

A

Y

A

N

N

C

C

I

L

D

D

R

R

H

H

L

L

K

Q

S

E

R

N

G

G

D

D

K

R

V

T

A

A

I

I

W
|
W

E

E

G

G

D

D

N

D

P

T

Y

S

I

Q

D

S

K

K

K

H

I

I

T

S

Y

Y

R

R

E

E

L

L

Y

H

E

R

N

D

V

V

C

C

R

R

M

F

A

A

N

N

V

T

L

L

K

L

K

D

H

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

T

A

I

I

Y

Y

L

M

P

P

M

M

I

V

P

P

E
|
E

A

A

Y

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

S

S

V

V

V

I

F
|
F

A
x
G

G
|
G

F

F

S

S

P

P

E

E

A

A

L

V

A

A

G
|
G

R

R

I

I

V

I

D

D

C

S

E

S

S

S

T

R

F

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R
|
R

G

A

G

G

K

R

P

S

V

I

A

P

L

L

K

K

E

K

N

N

T

V

D

D

T

D

A

A

I

L

D

K

I

-

A

-

A

N

K

P

Q

N

Y

V

V

T

M

S

V

V

N

E

K

H

V

V

L

I

V

V

V

L

R

K

R

R

T

T

G

G

G

S

K

D

V

I

S

D

W

W

G

Q

R

E

G

G

R

R

D

D

L

L

W

W

Y

W

H

R

Q

D

E

L

V

I

A

E

S

K

V

A

E

S

P

P

H

E

C

H

E

Q

P

P

E

E

P

A

M

M

N

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K
|
K

P
|
P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

L

L

V

V

Y

Y

A

A

S

A

M

T

T

T

H

F

Q

K

Y

Y

V

V

F

F

D

D

Y

Y

H

H

D

P

G

G

E

D

I

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

T

T

L

L

M

M

F

F

E

E

G

G

V

V

P

P

N

N

F

W

P

P

D

T

Q

P

G

A

R

R

F

M

W

C

E

Q

V

V

D

D

K

K

H

H

H

Q

V

V

N

N

I

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A

A

L

I

R

R

A

A

L

L

M

M

G

A

A

E

G

G

D

D

E

K

F

A

V

I

T

E

R

G

S

T

S

D

R

R

S

S

T

S

L

L

R

R

L

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

E

E

W

W

Y

Y

Y

W

N

K

V

K

V

I

G

G

D

K

Q

E

K

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

N

T

G

G

I

F

L

M

I

I

T

T

P

P

L

L

P

P

G

G

A

A

T

I

D

E

L

L

K

K

P

A

G

G

S

S

A

A

T

T

R

R

P

P

F

F

G

G

V

V

K

Q

P

P

V

A

L

L

V

V

D

D

N

N

E

E

G

G

N

H

V

P

Q

Q

E

E

G

G

V

A

A

T

D

E

G

G

N

N

L

L

C

V

I

I

S

T

D

D

S

S

W

W

P

P

G

G

Q

Q

M

A

R

R

T

T

V

L

Y

F

G

G

D

D

H

H

K

E

R

R

F

F

I

E

E

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

K

K

G

N

M

M

Y

Y

F

F

S

S

G

G

D
|
D

G

G

C

A

R

R

D

D

E

E

D

D

G

G

Y

Y

W

W

I
|
I

T

T

G

G

R

R

V

V

D

D

V

V

L

L

N
|
N

I

V

S
|
S

G

G

H

H

R
|
R

L

L

G

G

T

T

A

A

E

E

I

I

E

E

S

S

A

A

L

L

V
|
V

S

A

H
|
H

H

P

S

K

V
x
I

S

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

P

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

C

A

Y

Y

V

V

T

T

L

L

M

N

T

H

G

G

A

E

D

E

A

P

Q

S

D

-

P

-

D

P

E

E

L

L

R

Y

K

A

E

E

L

V

V

R

Q

N

H

W

V

V

R
|
R

K

K

E

E

I

I

G

G

P
|
P

I

L

A

A

T

T

P

P

D

D

K

V

I

L

Q

H

F

W

A

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

D

-

E

-

F

-

G

G

A

D

L

L

G

G

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

G

G

V

V

D

E

D

K

L

L

I

L

E

E

N

E

R

K

Q

Q

active site: T260 (= T264), T412 (= T416), E413 (= E417), N517 (= N521), R522 (= R526), K605 (= K611)
binding (r,r)-2,3-butanediol: W93 (= W95), E140 (= E142), G169 (= G171), K266 (= K270), P267 (= P271)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G387), E384 (= E388), P385 (= P389), T408 (= T412), W409 (= W413), W410 (= W414), Q411 (= Q415), T412 (= T416), D496 (= D500), I508 (= I512), N517 (= N521), R522 (= R526)
binding coenzyme a: F159 (= F161), G160 (≠ A162), G161 (= G163), R187 (= R189), S519 (= S523), R580 (= R586), P585 (= P591)
binding magnesium ion: V533 (= V537), H535 (= H539), I538 (≠ V542)

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
66% identity, 97% coverage: 17:648/651 of query aligns to 14:640/641 of 2p20A

query
sites
2p20A

L

L

I

I

D

N

N

P

E

E

T

Q

Y

Y

L

E

E

T

W

K

Y

Y

E

K

E

Q

S

S

V

I

S

N

D

D

P

P

D

D

G

T

F

F

W

W

A

G

K

E

H

Q

G

G

R

K

R

I

I

L

D

D

W

W

F

I

K

T

P

P

F

Y

T

Q

K

K

V

V

K

K

N

N

T

T

D

S

F

F

N

A

-

P

G

G

D

N

V

V

T

S

I

I

K

K

W

W

Y

Y

E

E

D

D

G

G

V

T

T

L

N

N

V

L

S

A

Y

A

N

N

C

C

I

L

D

D

R

R

H

H

L

L

K

Q

S

E

R

N

G

G

D

D

K

R

V

T

A

A

I

I

W

W

E

E

G

G

D

D

N

D

P

T

Y

S

I

Q

D

S

K

K

K

H

I

I

T

S

Y

Y

R

R

E

E

L

L

Y

H

E

R

N

D

V

V

C

C

R

R

M

F

A

A

N

N

V

T

L

L

K

L

K

D

H

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

T

A

I

I

Y

Y

L

M

P

P

M

M

I

V

P

P

E

E

A

A

Y

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

S

S

V

V

V

I

F

F

A

G

G

G

F

F

S

S

P

P

E

E

A

A

L

V

A

A

G

G

R

R

I

I

V

I

D

D

C

S

E

S

S

S

T

R

F

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R

R

G

A

G

G

K

R

P

S

V

I

A

P

L

L

K

K

E

K

N

N

T

V

D

D

T

D

A

A

I

L

D

K

I

-

A

-

A

N

K

P

Q

N

Y

V

V

T

M

S

V

V

N

E

K

H

V

V

L

I

V

V

V

L

R

K

R

R

T

T

G

G

G

S

K

D

V

I

S

D

W

W

G

Q

R

E

G

G

R

R

D

D

L

L

W

W

Y

W

H

R

Q

D

E

L

V

I

A

E

S

K

V

A

E

S

P

P

H

E

C

H

E

Q

P

P

E

E

P

A

M

M

N

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

L

L

V

V

Y

Y

A

A

S

A

M

T

T

T

H

F

Q

K

Y

Y

V

V

F

F

D

D

Y

Y

H

H

D

P

G

G

E

D

I

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

T

T

L

L

M

M

F

F

E

E

G

G

V

V

P

P

N

N

F

W

P

P

D

T

Q

P

G

A

R

R

F

M

W

C

E

Q

V

V

D

D

K

K

H

H

H

Q

V

V

N

N

I

I

F

L

Y

Y

T

T

A

A

P

P

T

T

A

A

L

I

R

R

A

A

L

L

M

M

G

A

A

E

G

G

D

D

E

K

F

A

V

I

T

E

R

G

S

T

S

D

R

R

S

S

T

S

L

L

R

R

L

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

E

E

W

W

Y

Y

Y

W

N

K

V

K

V

I

G

G

D

K

Q

E

K

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

N

T

G

G

I

F

L

M

I

I

T

T

P

P

L

L

P

P

G

G

A

A

T

I

D

E

L

L

K

K

P

A

G

G

S

S

A

A

T

T

R

R

P

P

F

F

G

G

V

V

K

Q

P

P

V

A

L

L

V

V

D

D

N

N

E

E

G

G

N

H

V

P

Q

Q

E

E

G

G

V

A

A

T

D

E

G

G

N

N

L

L

C

V

I

I

S

T

D

D

S

S

W

W

P

P

G

G

Q

Q

M

A

R

R

T

T

V

L

Y

F

G

G

D

D

H

H

K

E

R

R

F

F

I

E

E

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

K

K

G

N

M

M

Y

Y

F

F

S

S

G

G

D
|
D

G

G

C

A

R

R

D

D

E

E

D

D

G

G

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

L

L

N
|
N

I

V

S

S

G

G

H

H

R
|
R

L

L

G

G

T

T

A

A

E

E

I

I

E

E

S

S

A

A

L

L

V

V

S

A

H

H

H

P

S

K

V

I

S

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

P

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

C

A

Y

Y

V

V

T

T

L

L

M

N

T

H

G

G

A

E

D

E

A

P

Q

S

D

-

P

-

D

P

E

E

L

L

R

Y

K

A

E

E

L

V

V

R

Q

N

H

W

V

V

R

A

K

K

E

E

I

I

G

G

P

P

I

L

A

A

T

T

P

P

D

D

K

V

I

L

Q

H

F

W

A

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

D

G

E

D

F

T

G

S

A

-

L

-

G

N

D

D

T

T

S

S

T

T

L

L

A

A

D

D

P

P

G

G

V

V

D

E

D

K

L

L

I

L

E

E

N

E

R

K

Q

Q

active site: T260 (= T264), T412 (= T416), E413 (= E417), N517 (= N521), R522 (= R526), K605 (= K611)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G387), E384 (= E388), P385 (= P389), T408 (= T412), W409 (= W413), W410 (= W414), Q411 (= Q415), T412 (= T416), D496 (= D500), I508 (= I512), R511 (= R515), R522 (= R526)

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
66% identity, 97% coverage: 17:648/651 of query aligns to 13:636/637 of 2p2fA

query
sites
2p2fA

L

L

I

I

D

N

N

P

E

E

T

Q

Y

Y

L

E

E

T

W

K

Y

Y

E

K

E

Q

S

S

V

I

S

N

D

D

P

P

D

D

G

T

F

F

W

W

A

G

K

E

H

Q

G

G

R

K

R

I

I

L

D

D

W

W

F

I

K

T

P

P

F

Y

T

Q

K

K

V

V

K

K

N

N

T

T

D

S

F

F

N

A

-

P

G

G

D

N

V

V

T

S

I

I

K

K

W

W

Y

Y

E

E

D

D

G

G

V

T

T

L

N

N

V

L

S

A

Y

A

N

N

C

C

I

L

D

D

R

R

H

H

L

L

K

Q

S

E

R

N

G

G

D

D

K

R

V

T

A

A

I

I

W

W

E

E

G

G

D

D

N

D

P

T

Y

S

I

Q

D

S

K

K

K

H

I

I

T

S

Y

Y

R

R

E

E

L

L

Y

H

E

R

N

D

V

V

C

C

R

R

M

F

A

A

N

N

V

T

L

L

K

L

K

D

H

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

T

A

I

I

Y

Y

L

M

P

P

M

M

I

V

P

P

E

E

A

A

Y

V

A

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

V

V

H

H

S

S

V

V

V

I

F
|
F

A

G

G

G

F

F

S

S

P

P

E

E

A

A

L

V

A

A

G

G

R

R

I

I

V

I

D

D

C

S

E

S

S

S

T

R

F

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R
|
R

G

A

G

G

K

R

P

S

V

I

A

P

L

L

K

K

E

K

N

N

T

V

D

D

T

D

A

A

I

L

D

K

I

-

A

-

A

N

K

P

Q

N

Y

V

V

T

M

S

V

V

N

E

K

H

V

V

L

I

V

V

V

L

R

K

R

R

T

T

G

G

G

S

K

D

V

I

S

D

W

W

G

Q

R

E

G

G

R

R

D

D

L

L

W

W

Y

W

H

R

Q

D

E

L

V

I

A

E

S

K

V

A

E

S

P

P

H

E

C

H

E

Q

P

P

E

E

P

A

M

M

N

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

L

L

V

V

Y

Y

A

A

S

A

M

T

T

T

H

F

Q

K

Y

Y

V

V

F

F

D

D

Y

Y

H

H

D

P

G

G

E

D

I

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W
|
W

V

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

T

T

L

L

M

M

F

F

E

E

G

G

V

V

P
|
P

N

N

F

W

P

P

D

T

Q

P

G

A

R

R

F

M

W

C

E

Q

V

V

D

D

K

K

H

H

H

Q

V

V

N

N

I

I

F

L

Y

Y

T

T

A
|
A

P

P

T

T

A
|
A

L

I

R

R

A

A

L

L

M

M

G

A

A

E

G

G

D

D

E

K

F

A

V

I

T

E

R

G

S

T

S

D

R

R

S

S

T

S

L

L

R

R

L

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

E

E

W

W

Y

Y

Y

W

N

K

V

K

V

I

G

G

D

K

Q

E

K

K

C

C

P

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

N

T

G

G

I

F

L

M

I

I

T

T

P

P

L

L

P

P

G

G

A

A

T

I

D

E

L

L

K

K

P

A

G

G

S

S

A

A

T

T

R

R

P

P

F

F

G

G

V

V

K

Q

P

P

V

A

L

L

V

V

D

D

N

N

E

E

G

G

N

H

V

P

Q

Q

E

E

G

G

V

A

A

T

D

E

G

G

N

N

L

L

C

V

I

I

S

T

D

D

S

S

W

W

P

P

G

G

Q

Q

M

A

R

R

T

T

V

L

Y

F

G

G

D

D

H

H

K

E

R

R

F

F

I

E

E

Q

T

T

Y

Y

F

F

S

S

T

T

Y

F

K

K

G

N

M

M

Y

Y

F

F

S

S

G

G

D
|
D

G

G

C

A

R

R

D

D

E

E

D

D

G

G

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

L

L

N
|
N

I

V

S
|
S

G

G

H

H

R
|
R

L

L

G

G

T

T

A

A

E

E

I

I

E

E

S

S

A

A

L

L

V

V

S

A

H

H

H

P

S

K

V

I

S

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

P

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

C

A

Y

Y

V

V

T

T

L

L

M

N

T

H

G

G

A

E

D

E

A

P

Q

S

D

-

P

-

D

P

E

E

L

L

R

Y

K

A

E

E

L

V

V

R

Q

N

H

W

V

V

R
|
R

K

K

E

E

I

I

G

G

P
|
P

I

L

A

A

T

T

P

P

D

D

K

V

I

L

Q

H

F

W

A

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

D

-

E

-

F

-

G

-

A

-

L

-

G

G

D

D

T

T

-

S

S

S

T

T

L

L

A

A

D

D

P

P

G

G

V

V

D

E

D

K

L

L

I

L

E

E

N

E

R

K

Q

Q

active site: T259 (= T264), T411 (= T416), E412 (= E417), N516 (= N521), R521 (= R526), K604 (= K611)
binding adenosine monophosphate: G382 (= G387), E383 (= E388), P384 (= P389), T407 (= T412), W408 (= W413), W409 (= W414), Q410 (= Q415), T411 (= T416), D495 (= D500), I507 (= I512), R510 (= R515), N516 (= N521), R521 (= R526)
binding coenzyme a: F158 (= F161), R186 (= R189), W304 (= W309), T306 (= T311), P329 (= P334), A352 (= A357), A355 (= A360), S518 (= S523), R579 (= R586), P584 (= P591)

1pg3A Acetyl coa synthetase, acetylated on lys609 (see paper)
66% identity, 97% coverage: 17:648/651 of query aligns to 14:633/634 of 1pg3A

query
sites
1pg3A

L

L

I

I

D

N

N

P

E

E

T

Q

Y

Y

L

E

E

T

W

K

Y

Y

E

K

E

Q

S

S

V

I

S

N

D

D

P

P

D

D

G

T

F

F

W

W

A

G

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E

H

Q

G

G

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R

I

I

L

D

D

W

W

F

I

K

T

P

P

F

Y

T

Q

K

K

V

V

K

K

N

N

T

T

D

S

F

F

N

A

-

P

G

G

D

N

V

V

T

S

I

I

K

K

W

W

Y

Y

E

E

D

D

G

G

V

T

T

L

N

N

V

L

S

A

Y

A

N

N

C

C

I

L

D

D

R

R

H

H

L

L

K

Q

S

E

R

N

G

G

D

D

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R

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T

A

A

I

I

W

W

E

E

G

G

D

D

N

D

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Q

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S

K

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H

I

I

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S

Y

Y

R

R

E

E

L

L

Y

H

E

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N

D

V

V

C

C

R

R

M

F

A

A

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T

L

L

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D

H

L

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G

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I

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K

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G

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A

I

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Y

Y

L

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P

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M

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E

E

A

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V

A

A

M

M

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L

A

A

C

C

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A

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G

A

A

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H

H

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S

V

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F

A
x
G

G

G

F

F

S

S

P

P

E

E

A

A

L

V

A

A

G

G

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C

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I

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A

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G

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R

G

A

G

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K
x
R

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S

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I

A

P

L

L

K

K

E

K

N

N

T

V

D

D

T

D

A

A

I

L

D

K

I

-

A

-

A

N

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N

Y

V

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M

S

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V

N

E

K

H

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I

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K

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T

G

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K

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G

G

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R

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D

L

L

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W

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L

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I

A

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K

V

A

E

S

P

P

H

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C

H

E

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P

P

E

E

P

A

M

M

N

N

A

A

E

E

D

D

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P

L

L

F

F

I

I

L

L

Y

Y

T
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T

S

S

G

G

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S

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T

G

G

K

K

P

P

K

K

G

G

V

V

L

L

H

H

T

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G

G

Y

Y

L

L

V

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Y

Y

A

A

S

A

M

T

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F

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K

Y

Y

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V

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F

D

D

Y

Y

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H

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P

G

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I

I

Y

Y

W

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C

C

T

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A
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A

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D

V

V

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G

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W

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T

G

G

H

H

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S

Y

Y

I

L

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L

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Y

G

G

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P

L

L

A

A

N

C

G

G

A

A

T

T

L

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M

M

F

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G

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P

N

N

F

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R

F

M

W

C

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D

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K

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V

N

N

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A
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A

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R

A

A

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M

M

G

A

A

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F

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P

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I

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N

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P

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E

A

A

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W

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T

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W

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E

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G

G

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A

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F

G

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A

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L

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D

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E

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K

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Y

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D

G

G

C

A

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R

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Y

W

W

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G

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R

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L

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N

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S

G

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R
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R

L

L

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G

T

T

A

A

E

E

I

I

E

E

S

S

A

A

L

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V
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V

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A

H
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H

H

P

S

K

V
x
I

S

A

E

E

A

A

V

V

G

G

Y

I

P

P

H

H

P

A

I

I

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K

G

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Q

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A

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I

Y

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A

Y

Y

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T

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M

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T

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G

G

A

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D

E

A

P

Q

S

D

-

P

-

D

P

E

E

L

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N

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R
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R

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K

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E

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G

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P

I

L

A

A

T

T

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D

K

V

I

L

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H

F

W

A

T

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D

G

S

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L

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P

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K

T

T

R

R

S

S

G

G

K
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K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

E

A

D

-

E

-

F

-

G

-

A

-

L

-

G

G

D

D

T

-

S

-

T

-

L

L

A

A

D

D

P

P

G

G

V

V

D

E

D

K

L

L

I

L

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N

E

R

K

Q

Q

active site: T260 (= T264), T412 (= T416), E413 (= E417), N517 (= N521), R522 (= R526), K605 (= K611)
binding coenzyme a: F159 (= F161), G160 (≠ A162), R187 (= R189), R190 (≠ K192), A301 (= A305), T307 (= T311), P330 (= P334), A356 (= A360), S519 (= S523), R580 (= R586), P585 (= P591)
binding magnesium ion: V533 (= V537), H535 (= H539), I538 (≠ V542)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G387), E384 (= E388), P385 (= P389), T408 (= T412), W409 (= W413), W410 (= W414), Q411 (= Q415), T412 (= T416), D496 (= D500), R511 (= R515), R522 (= R526)

Q9NR19 Acetyl-coenzyme A synthetase, cytoplasmic; Acetate--CoA ligase; Acetyl-CoA synthetase; ACS; AceCS; Acetyl-CoA synthetase 1; AceCS1; Acyl-CoA synthetase short-chain family member 2; Acyl-activating enzyme; Propionate--CoA ligase; EC 6.2.1.1; EC 6.2.1.17 from Homo sapiens (Human) (see paper)
53% identity, 98% coverage: 10:647/651 of query aligns to 33:696/701 of Q9NR19

query
sites
Q9NR19

P
|
P

E
|
E

A
x
V

K
x
S

K
x
R

N
x
S

T
x
A

L
x
H

I
x
V

D
x
P

N
x
S

-
x
L

E
x
Q

T
x
R

Y
|
Y

L
x
R

E
|
E

W
x
L

Y
x
H

E
x
R

S
|
S

V
|
V

S
x
E

D
x
E

P
|
P

D
x
R

G
x
E

F
|
F

W
|
W