SitesBLAST

C9 (≠ F9) mutation to S: No effect.
C20 (= C20) mutation to S: Loss of oligomerization; no alteration of water permeability.
T183 (= T181) mutation to C: No effect.
R189 (= R187) mutation R->V,S: Loss of function.

8uy6A Aquaporin z with alfa tag and bound to nanobody (see paper)
71% identity, 91% coverage: 1:207/228 of query aligns to 1:209/244 of 8uy6A

query
sites
8uy6A

M

M

L

F

N

R

K

K

L

L

S

A

A

A

E

E

F
x
C

F
|
F

G

G

T

T

F
|
F

W

W

L

L

V

V

F

F

G

G

C

C

G

G

S

S

A

A

I

V

L

L

A

A

A

G

F

F

P

P

E

E

L

L

G

G

I

I

G

G

F

F

L

A

G

G

V

V

A

A

L

L

A

A

F

F

G

G

L

L

T

T

V

V

L

L

T

T

M

M

A

A

Y

F

A

A

V

V

G

G

G

H

I

I

S

S

G

G

H

H

F

F

N

N

P

P

A

A

V

V

S

T

L

I

G

G

L

L

T

W

V

A

A

G

G

G

R

R

L

F

P

P

A

A

K

K

D

E

L

V

I

V

P

G

Y

Y

W

V

V

I

A

A

Q

Q

V

V

L

V

G

G

A

G

I
|
I

A
x
V

A

A

I

L

L

L

Y
|
Y

V

L

I

I

A

A

S

S

G

G

K

K

D

T

G

G

F

F

-

D

-

A

S

A

A

A

G

S

G

G

L

F

A

A

S

S

N

N

G

G

Y

Y

G

G

E

E

L

H

S

S

P

P

G

G

Y

Y

S

S

M

M

M

L

A

S

G

A

L

L

L

V

I

V

E

E

I

L

I

V

L

L

T

S

A

A

F

G

F

F

I

L

I

V

I

I

L

H

G

G

S

A

T

T

S

D

S

K

L

F

A

A

P

P

A

A

G

G

F

F

A

A

P

P

I

I

A

A

I

I

G

G

F

L

G

A

L

L

T

T

L

L

I

I

H

H

L

L

V

I

S

S

I

I

P

P

V

V

T

T

N

N

T

T

S

S

V

V

N

N

P

P

A

A

R

R

S

S

T

T

G

A

V

V

A

A

L
x
I

F
|
F

A

Q

D

G

T

G

A

W

A

A

L

L

S

E

Q

Q

L

L

W

W

L

F

F

F

W
|
W

binding cardiolipin: C9 (≠ F9), F10 (= F10), F13 (= F13), I93 (= I93), V94 (≠ A94), Y100 (= Y100), I195 (≠ L193), F196 (= F194), W209 (= W207)

2o9eA Crystal structure of aqpz mutant t183c complexed with mercury (see paper)
67% identity, 100% coverage: 1:228/228 of query aligns to 3:232/232 of 2o9eA

query
sites
2o9eA

M

M

L

F

N

R

K

K

L

L

S

A

A

A

E

E

F

S

F

F

G

G

T

T

F

F

W

W

L

L

V

V

F

F

G

G

C

S

G

G

S

S

A

A

I

V

L

L

A

A

A

G

F

F

P

P

E

E

L

L

G

G

I

I

G

G

F

F

L

A

G

G

V

V

A

A

L

L

A

A

F

F

G

G

L

L

T

T

V

V

L

L

T

T

M

M

A

A

Y

F

A

A

V

V

G

G

G

H

I

I

S

S

G

G

H

H

F

F

N

N

P

P

A

A

V

V

S

T

L

I

G

G

L

L

T

W

V

A

A

G

G

G

R

R

L

F

P

P

A

A

K

K

D

E

L

V

I

V

P

G

Y

Y

W

V

V

I

A

A

Q

Q

V

V

L

V

G

G

A

G

I

I

A

V

A

A

I

L

L

L

Y

Y

V

L

I

I

A

A

S

S

G

G

K

K

D

T

G

G

F

F

-

D

-

A

S

A

A

A

G

S

G

G

L

F

A

A

S

S

N

N

G

G

Y

Y

G

G

E

E

L

H

S

S

P

P

G

G

Y

Y

S

S

M

M

M

L

A

S

G

A

L

L

L

V

I

V

E
|
E

I

L

I

V

L

L

T

S

A

A

F

G

F

F

I

L

I

V

I

I

L

H

G

G

S

A

T

T

S

D

S

K

L

F

A

A

P

P

A

A

G

G

F

F

A

A

P

P

I

I

A

A

I

I

G

G

F

L

G

A

L

L

T

T

L

L

I

I

H

H

L

L

V

I

S
|
S

I

I

P

P

V

V

T

T

N

N

T

C

S

S

V

V

N

N

P

P

A

A

R

R

S

S

T

T

G

A

V

V

A

A

L

I

F

F

A

Q

D

G

T

G

A

W

A

A

L

L

S

E

Q

Q

L

L

W

W

L

F

F

F

W

W

V

V

A

V

P

P

L

I

V

V

G

G

A

G

V

I

I

I

G

G

A

G

I

L

I

I

W

Y

K

R

G

T

L

L

G

E

R

K

D

R

binding mercury (ii) ion: E140 (= E136), S179 (= S175)

3nkaA Crystal structure of aqpz h174g,t183f (see paper)
70% identity, 91% coverage: 1:207/228 of query aligns to 3:211/230 of 3nkaA

query
sites
3nkaA

M

M

L

F

N

R

K

K

L

L

S

A

A

A

E

E

F

C

F

F

G

G

T

T

F

F

W

W

L

L

V

V

F

F

G

G

C

C

G

G

S

S

A
|
A

I

V

L

L

A

A

A

G

F

F

P

P

E

E

L

L

G

G

I

I

G

G

F

F

L

A

G

G

V
|
V

A

A

L

L

A

A

F

F

G

G

L

L

T

T

V

V

L

L

T

T

M

M

A

A

Y

F

A

A

V

V

G

G

G

H

I

I

S

S

G

G

H

H

F

F

N

N

P

P

A

A

V

V

S

T

L

I

G

G

L

L

T

W

V

A

A

G

G

G

R

R

L

F

P

P

A

A

K

K

D

E

L

V

I

V

P

G

Y

Y

W

V

V

I

A

A

Q

Q

V

V

L

V

G

G

A

G

I

I

A

V

A

A

I

L

L

L

Y

Y

V

L

I

I

A

A

S

S

G

G

K

K

D

T

G

G

F

F

-

D

-

A

S

A

A

A

G

S

G

G

L

F

A

A

S

S

N

N

G

G

Y

Y

G

G

E

E

L

H

S

S

P

P

G

G

Y

Y

S

S

M

M

M

L

A

S

G

A

L

L

L

V

I

V

E

E

I

L

I

V

L

L

T

S

A

A

F

G

F

F

I

L

I

V

I

I

L

H

G

G

S

A

T

T

S

D

S

K

L

F

A

A

P

P

A

A

G

G

F

F

A

A

P

P

I

I

A

A

I

I

G

G

F

L

G

A

L

L

T

T

L

L

I

I

H

G

L

L

V

I

S

S

I
|
I

P

P

V

V

T

T

N
|
N

T

F

S

S

V

V

N

N

P

P

A

A

R

R

S

S

T

T

G

A

V

V

A

A

L

I

F

F

A

Q

D

G

T

G

A

W

A

A

L

L

S

E

Q

Q

L

L

W

W

L

F

F

F

W

W

binding glycerol: A25 (= A23), V41 (= V39), I180 (= I176), N184 (= N180)

P08995 Nodulin-26; N-26 from Glycine max (Soybean) (Glycine hispida) (see paper)
38% identity, 97% coverage: 2:222/228 of query aligns to 37:245/271 of P08995

query
sites
P08995

L

L

N

Q

K

K

L

L

S

V

A

A

E

E

F

A

F

V

G

G

T

T

F

Y

W

F

L

L

V

I

F

F

G

A

G

G

C

C

G

A

S

S

A

L

I

V

L

V

A

N

A

E

A

N

F

Y

P

Y

E

N

L

M

G

-

I

I

G

T

F

F

L

P

G

G

V

I

A

A

L

I

A

V

F

W

G

G

L

L

T

V

V

L

L

T

T

V

M

L

A

V

Y

Y

A

T

V

V

G

G

G

H

I

I

S

S

G

G

H

H

F

F

N

N

P

P

A

A

V

V

S

T

L

I

G

A

L

F

T

A

V

S

A

T

G

R

R

R

L

F

P

P

A

L

K

I

D

Q

L

V

I

P

P

A

Y

Y

W

V

V

V

A

A

Q

Q

V

L

L

L

G

G

A

S

I

I

A

L

A

A

A

S

A

G

I

T

L

L

Y

R

V

L

I

L

A

F

S

M

G

G

K

N

-

H

D

D

G

Q

F

F

S

S

A

-

G

-

G

G

L

T

A

V

S

P

N

N

G

G

Y

-

G

-

E

-

L

-

S

-

P

-

G

-

Y

-

S

T

M

N

M

L

A

Q

G

A

L

F

L

V

I

F

E

E

I

F

I

I

L

M

T

T

A

F

F

F

F

L

I

M

I

F

I

V

I

I

L

C

G

G

-

V

S

A

T

T

S

D

S

N

L

R

A

A

P

V

A

G

G

E

F

F

A

A

P

G

I

I

A

A

I

I

G

G

F

S

G

T

L

L

T

L

L

L

I

N

H

V

L

I

V

I

S

G

I

G

P

P

V

V

T

T

N

G

T

A

S

S

V

M

N

N

P

P

A

A

R

R

S

S

T

L

G

G

V

P

A

A

L

F

F

V

A

H

D

-

T

-

A

G

A

E

L

Y

S

E

Q

G

L

I

W

W

L

I

F

Y

W

L

V

L

A

A

P

P

L

V

V

V

G

G

A

A

V

I

I

A

G

G

A

A

I

W

I

V

W

Y

K

N

Sites not aligning to the query:

262 modified: Phosphoserine; by CPK

P55064 Aquaporin-5; AQP-5 from Homo sapiens (Human) (see 3 papers)
41% identity, 98% coverage: 2:225/228 of query aligns to 11:225/265 of P55064

query
sites
P55064

L

L

N

K

K

A

L

V

S

F

A

A

E

E

F

F

F

L

G

A

T

T

F

L

W

I

L

F

V

V

F

F

G

F

G

G

C

L

G

G

S

S

A

A

I

L

L

K

-

W

A

P

A

S

A
|
A

F

L

P

P

E

T

L

I

G

-

I

-

G

-

F

-

L

L

G

Q

V
x
I

A

A

L

L

A

A

F

F

G

G

L

L

T

A

V

I

L

G

T

T

M

L

A

A

Y

Q

A

A

V

L

G

G

G

P

I

V

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

I

S

T

L

L

G

A

L

L

T

L

V

V

A

G

G

N

R

Q

L

I

P

S

A

L

K

L

D

R

L

A

I

F

P

F

Y

Y

W

V

V

A

A

A

Q

Q

V

L

L

V

G

G

A

A

I

I

A

A

A

G

A

A

A

G

I

I

L

L

Y

Y

V

G

I

V

A

A

S

P

G

L

K

N

D

-

G

-

F

-

S

A

A

R

G

G

G

N

L

L

A

A

S

V

N

N

G

A

Y

L

G
x
N

E

N

L

N

S

T

P

T

G

Q

G

G

Y

Q

S

A

M

M

M

V

A

-

G

-

L

-

I

V

E

E

I

L

I

I

L

L

T

T

A

F

F

Q

F

L

I

A

I

L

I

C

I

I

L

F

G

A

S

S

T

T

S

D

S

S

L

R

-

R

-

T

A
x
S

P

P

A

V

G

G

F

S

A

P

P

A

I

L

A

S

I

I

G

G

F

L

G

S

L

V

T

T

L

L

I

G

H

H

L

L

V

V

S

G

I
|
I

P

Y

V

F

T

T

N

G

T

C

S

S

V

M

N

N

P

P

A

A

R
|
R

S

S

T

F

G

G

V

P

A

A

L

V

F

V

A

M

D

N

T

R

A

F

A

S

L

P

S

A

Q

H

L

-

W

W

L

V

F

F

W

W

V

V

A

G

P

P

L

I

V

V

G

G

A

A

V

V

I

L

G

A

A

A

I

I

I

L

W

Y

K

F

G

Y

L

L

A38 (= A28) to E: in PPKB; no effect on localization to plasma membrane; dbSNP:rs398123054
I45 (≠ V39) to S: in PPKB; no effect on localization to plasma membrane; dbSNP:rs398123055
N123 (≠ G120) to D: in PPKB; no effect on localization to plasma membrane; dbSNP:rs398123057; to Y: in PPKB; changed water channel activity; the channel is either leaky and/or more sensitive to hypotonicity; associated with increased TRPV4 basal activity
S156 (≠ A155) mutation to A: No effect on localization to plasma membrane.; mutation to E: Increased localization to plasma membrane.
I177 (= I176) to F: in PPKB; no effect on localization to plasma membrane; dbSNP:rs398123056
R188 (= R187) to C: in PPKB; no effect on localization to plasma membrane; dbSNP:rs368292687

5dyeD Crystal structure of the full length s156e mutant of human aquaporin 5 (see paper)
41% identity, 98% coverage: 2:225/228 of query aligns to 10:224/253 of 5dyeD

query
sites
5dyeD

L

L

N

K

K

A

L

V

S

F

A

A

E

E

F

F

F

L

G

A

T

T

F

L

W

I

L

F

V

V

F

F

G

F

G

G

C

L

G

G

S

S

A

A

I

L

L

K

-

W

A

P

A

S

A

A

F

L

P

P

E

T

L

I

G

-

I

-

G

-

F

-

L

L

G

Q

V

I

A

A

L

L

A

A

F

F

G

G

L

L

T

A

V

I

L

G

T

T

M

L

A

A

Y

Q

A

A

V

L

G

G

G

P

I

V

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

I

S

T

L

L

G

A

L

L

T

L

V

V

A

G

G

N

R

Q

L

I

P

S

A

L

K

L

D

R

L

A

I

F

P

F

Y

Y

W

V

V

A

A

A

Q

Q

V

L

L

V

G

G

A

A

I

I

A

A

A

G

A

A

A

G

I

I

L

L

Y

Y

V

G

I

V

A

A

S

P

G

L

K

N

D

-

G

-

F

-

S

A

A

R

G

G

G

N

L

L

A

A

S

V

N

N

G

A

Y

L

G

N

E

N

L

N

S

T

P

T

G

Q

G

G

Y

Q

S

A

M

M

M

V

A

-

G

-

L

-

I

V

E

E

I

L

I

I

L

L

T

T

A

F

F

Q

F

L

I

A

I

L

I

C

I

I

L

F

G

A

S

S

T

T

S

D

S

S

L

R

-

R

-

T

A

E

P

P

A

V

G
|
G

F
x
S

A

P

P

A

I

L

A

S

I

I

G

G

F

L

G

S

L

V

T

T

L

L

I

G

H

H

L

L

V

V

S

G

I

I

P

Y

V

F

T

T

N

G

T

C

S

S

V

M

N

N

P

P

A

A

R

R

S

S

T

F

G

G

V

P

A

A

L

V

F

V

A

M

D

N

T

R

A

F

A

S

L

P

S

A

Q

H

L

-

W

W

L

V

F

F

W

W

V

V

A

G

P

P

L

I

V

V

G

G

A

A

V

V

I

L

G

A

A

A

I

I

I

L

W

Y

K

F

G

Y

L

L

binding O-[(S)-{[(2S)-2-(hexanoyloxy)-3-(tetradecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-D-serine: G158 (= G158), S159 (≠ F159)

Q6J8I9 Lens fiber major intrinsic protein; Aquaporin-0 from Ovis aries (Sheep) (see 2 papers)
38% identity, 96% coverage: 7:225/228 of query aligns to 15:223/263 of Q6J8I9

query
sites
Q6J8I9

A

A

E

E

F

F

G

A

T

T

F

L

W

F

L

Y

V

V

F

F

G

F

G

G

C

L

G

G

S

A

A

S

I

L

L

R

A

W

A

A

A

P

F

G

P

P

E

-

L

-

G

-

I

L

G

H

F

V

L

L

G

Q

V

V

A

A

L

L

A

A

F

F

G

G

L

L

T

A

V

L

L

A

T

T

M

L

A

V

Y

Q

A

A

V

V

G

G

G

H

I

I

S

S

G

G

G

A

H

H

F

V

N

N

P

P

A

A

V

V

S

T

L

F

G

A

L

F

T

L

V

V

A

G

G

S

R

Q

L

M

P

S

A

L

K

L

D

R

L

A

I

I

P

C

Y

Y

W

V

V

V

A

A

Q

Q

V

L

L

L

G

G

A

A

I

V

A

A

A

G

A

A

I

V

L

L

Y

Y

V

S

I

V

A

T

S

P

G

P

K

A

D

-

G

-

F

-

S

V

A

R

G

G

G

N

L

L

A

A

S

L

N

N

G

-

Y

-

G

-

E

T

L

L

S

H

P

P

G

-

G

G

Y

V

S

S

M

V

M

G

A

Q

G

A

L

T

L

I

I

V

E

E

I

I

I

F

L

L

T

T

A

L

F

Q

F

F

I

V

I

L

I

C

I

I

L

F

G

A

S

T

T
x
Y

S

D

S

E

L

R

A

R

P

N

A

G

-

R

-

L

G

G

F

S

A

V

P

A

I

L

A

A

I

V

G

G

F

F

G

S

L

L

T

T

L

L

I

G

H

H

L

L

V

F

S

G

I

M

P

Y

V

Y

T

T

N

G

T

A

S

G

V

M

N

N

P

P

A

A

R

R

S

S

T

F

G

A

V

P

A

A

L

I

F

L

A

-

D

-

T

T

A

R

A

N

L

F

S

T

Q

N

L

H

W

W

L

V

F

Y

W

W

V

V

A

G

P

P

L

V

V

I

G

G

A

A

V

G

I

L

G

G

A

S

I

L

W

Y

K

D

G

F

L

L

Y149 (≠ T151) mutation to G: Loss of inhibition by calcium.; mutation to L: Decreased water channel activity. Loss of inhibition by calcium.; mutation to S: Decreased water channel activity. Loss of inhibition by calcium.

Sites not aligning to the query:

234:235 Cleavage; promotes interactions between tetramers from adjoining membranes
243:244 Cleavage; promotes interactions between tetramers from adjoining membranes

P06624 Lens fiber major intrinsic protein; Aquaporin-0; MIP26; MP26 from Bos taurus (Bovine) (see 4 papers)
37% identity, 98% coverage: 3:225/228 of query aligns to 11:223/263 of P06624

query
sites
P06624

N

R

K

A

L

I

S

C

A

A

E

E

F

F

G

A

T

S

F

L

W

F

L

Y

V

V

F

F

G

F

G

G

C

L

G

G

S

A

A

S

I

L

L

R

A

W

A

A

A

P

F

G

P

P

E

-

L

-

G

-

I

L

G

H

F

V

L

L

G

Q

V

V

A

A

L

L

A

A

F

F

G

G

L

L

T

A

V

L

L

A

T

T

M

L

A

V

Y

Q

A

A

V

V

G

G

G

H

I

I

S

S

G

G

G

A

H

H

F

V

N

N

P

P

A

A

V

V

S

T

L

F

G

A

L

F

T

L

V

V

A

G

G

S

R

Q

L

M

P

S

A

L

K

L

D

R

L

A

I

I

P

C

Y

Y

W

M

V

V

A

A

Q

Q

V

L

L

L

G

G

A

A

I

V

A

A

A

G

A

A

I

V

L

L

Y

Y

V

S

I

V

A

T

S

P

G

P

K

A

D

-

G

-

F

-

S

V

A

R

G

G

G

N

L

L

A

A

S

L

N

N

G

-

Y

-

G

-

E

T

L

L

S

H

P

P

G

-

G

G

Y

V

S

S

M

V

M

G

A

Q

G

A

L

T

L

I

I

V

E

E

I

I

I

F

L

L

T

T

A

L

F

Q

F

F

I

V

I

L

I

C

I

I

L

F

G

A

S

T

T
x
Y

S

D

S

E

L

R

A

R

P

N

A

G

-

R

-

L

G

G

F

S

A

V

P

A

I

L

A

A

I

V

G

G

F

F

G

S

L

L

T

T

L

L

I

G

H

H

L

L

V

F

S

G

I

M

P

Y

V

Y

T

T

N

G

T

A

S

G

V

M

N

N

P

P

A

A

R

R

S

S

T

F

G

A

V

P

A

A

L

I

F

L

A

-

D

-

T

T

A

R

A

N

L

F

S

T

Q

N

L

H

W

W

L

V

F

Y

W

W

V

V

A

G

P

P

L

V

V

I

G

G

A

A

V

G

I

L

G

G

A

S

I

L

W

Y

K

D

G

F

L

L

Y149 (≠ T151) Important for water channel gating; mutation to G: Increases constitutive water permeability. Abolishes regulation by cytoplasmic calcium levels.; mutation to L: Strongly decreases water permeability. Abolishes regulation by cytoplasmic calcium levels.; mutation to S: Slightly decreases water permeability, but has a minor effect on the regulation by cytoplasmic calcium levels.

Sites not aligning to the query:

227 L→A: Strongly reduced CALM binding.
227:237 Interaction with CALM
230 V→A: Strongly reduced CALM binding.
235 modified: Phosphoserine
243 modified: Phosphoserine; by PKA
245 modified: Phosphoserine
246 Interaction with BFSP1; modified: Deamidated asparagine
250 interaction with BFSP1

Q9XF58 Aquaporin PIP2-5; Plasma membrane intrinsic protein 2-5; ZmPIP2-5; ZmPIP2;5; ZmPIP2a from Zea mays (Maize) (see paper)
38% identity, 85% coverage: 33:225/228 of query aligns to 77:270/285 of Q9XF58

query
sites
Q9XF58

G

G

I

V

G

G

F

V

L

L

G

G

V

I

A

A

L

W

A

A

F

F

G

G

L

G

T

M

V

I

L

F

T

I

M

L

A

V

Y

Y

A

C

V

T

G

A

G

G

I

V

S

S

G

G

G
|
G

H

H

F

I

N

N

P

P

A

A

V

V

S

T

L

F

G

G

L

L

T

F

V

L

A

A

G

R

R

K

L

V

P

S

A

L

K

V

D

R

L

A

I

L

P

L

Y

Y

W

I

V

V

A

A

Q

Q

V

C

L

L

G

G

A

A

I

I

A

C

A

G

A

V

A

G

I

L

L

V

Y

-

V

-

I

-

A

-

S

-

G

-

K

-

D

K

G

G

F

F

S

Q

A

S

G

A

G

F

L

Y

A

V

S

R

N

Y

G

G

Y

G

G

G

-

A

-

N

E

E

L

L

S

S

P

-

G

A

G

G

Y

Y

S

S

M

K

M

G

A

T

G

G

L

L

L

A

I

A

E

E

I

I

L

G

T

T

A

F

F

V

F

L

I

V

I

Y

I

T

I

V

L

F

G

S

S

A

T

T

S

D

S

P

L

K

A

R

P

N

A

A

G

R

-

D

-

S

-

H

-

V

-

P

-

V

F

L

A

A

P

P

I

L

A

P

I

I

G

G

F

F

G

A

L

V

T

F

L

M

I

V

H

H

L

L

V

A

S

T

I

I

P

P

V

I

T

T

N

G

T

T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

L

G

G

V

A

A

A

-

V

L

I

F

Y

A

N

D

N

T

D

A

K

A

A

L

W

S

D

Q

D

L

H

W

W

L

I

F

F

W

W

V

V

A

G

P

P

L

F

V

I

G

G

A

A

V

A

I

I

G

A

A

A

I

A

I

Y

W

H

K

Q

G

Y

L

V

L

L

G104 (= G60) mutation to W: Loss of water transport.

P29972 Aquaporin-1; AQP-1; Aquaporin-CHIP; Channel-like integral membrane protein of 28 kDa; Urine water channel from Homo sapiens (Human) (see 4 papers)
38% identity, 96% coverage: 7:225/228 of query aligns to 16:231/269 of P29972

query
sites
P29972

A

A

E

E

F

F

F

L

G

A

T

T

F

T

W

L

L

F

V

V

F

F

G

I

G

S

C

I

G

G

S

S

A

A

I

-

L

L

A

G

A

F

A

K

F

Y

P
|
P

-

V

-

G

-

N

E

Q

L

T

G
x
A

I

V

-

Q

G

D

F

N

L

V

G

K

V

V

A

S

L

L

A

A

F

F

G

G

L

L

T

S

V

I

L

A

T

T

M

L

A

A

Y

Q

A

S

V

V

G

G

G

H

I

I

S

S

G

G

G

A

H

H

F

L

N

N

P

P

A

A

V

V

S

T

L

L

G

G

L

L

T

L

V

L

A

S

G

C

R

Q

L

I

P

S

A

I

K

F

D

R

L

A

I

L

P

M

Y

Y

W

I

V

I

A

A

Q

Q

V

C

L

V

G

G

A

A

I

I

A

V

A

A

A

T

A

A

I

I

L

L

Y

S

V

G

I

I

A

T

S

S

G

S

K

L

D

T

G

G

F

N

S

S

A

L

G

-

L

-

A

-

S

-

N

-

G

G

Y

R

G

N

E

D

L

L

S

A

P

D

G

G

G

V

Y

N

S

S

M

G

M

Q

A

-

G

G

L

L

L

G

I

I

E

E

I

I

L

G

T

T

A

L

F

Q

F

L

I

V

I

L

I

C

I

V

L

L

G

A

S

T

T

T

S

D

S

R

L

R

A

R

P

R

A

D

-

L

-
x
G

G

G

F

S

A

A

P

P

I

L

A

A

I

I

G

G

F

L

G

S

L

V

T

A

L

L

I

G

H
|
H

L

L

V

L

S

A

I

I

P

D

V

Y

T

T

N

G

T
x
C

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

F

G

G

V

S

A

A

L

V

F

I

A

-

D

-

T

T

A

H

A

N

L

F

S

S

Q

N

L
x
H

W

W

L

I

F

F

W

W

V

V

A

G

P

P

L

F

V

I

G

G

A

G

V

A

I

L

G

A

A

V

I

L

I

I

W

Y

K

D

G

F

L

I

L

L

P38 (= P30) to L: in Co(A-B-) antigen; non functional AQP1; red cells show low osmotic water permeability; dbSNP:rs104894004
A45 (≠ G33) to V: in Co(A-B+) antigen; dbSNP:rs28362692
G165 (vs. gap) to D: in dbSNP:rs28362731
H180 (= H172) mutation to A: No effect on water channel activity.
C189 (≠ T181) Hg(2+)-sensitive residue; mutation to S: No effect on water channel activity.
H209 (≠ L203) mutation to A: Decreased water channel activity.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

8sjxA Structure of lens aquaporin-0 array in sphingomyelin/cholesterol bilayer (2sm:1chol) (see paper)
38% identity, 96% coverage: 7:225/228 of query aligns to 10:218/220 of 8sjxA

query
sites
8sjxA

A

A

E

E

F
|
F

F

F

G

A

T

T

F

L

W

F

L

Y

V

V

F

F

G

F

G

G

C

L

G

G

S

A

A

S

I

L

L

R

A

W

A

A

A

P

F

G

P

P

E

-

L

-

G

-

I

L

G

H

F

V

L

L

G

Q

V

V

A

A

L

L

A

A

F

F

G

G

L

L

T

A

V

L

L

A

T

T

M

L

A

V

Y

Q

A

A

V

V

G

G

G

H

I

I

S

S

G

G

G

A

H

H

F

V

N

N

P

P

A

A

V

V

S

T

L

F

G

A

L

F

T

L

V

V

A

G

G

S

R

Q

L

M

P

S

A

L

K

L

D

R

L

A

I
|
I

P

C

Y

Y

W

V

V
|
V

A

A

Q

Q

V
x
L

L
|
L

G

G

A

A

I

V

A

A

A

G

A

A

I

V

L

L

Y
|
Y

V
x
S

I

V

A

T

S

P

G

P

K

A

D

-

G

-

F

-

S

V

A

R

G

G

G

N

L

L

A

A

S

L

N

N

G

-

Y

-

G

-

E

T

L

L

S

H

P

P

G

-

G

G

Y

V

S

S

M

V

M

G

A

Q

G

A

L

T

L

I

I

V

E

E

I

I

I

F

L

L

T

T

A

L

F

Q

F

F

I

V

I

L

I

C

I

I

L

F

G

A

S

T

T

Y

S

D

S

E

L

R

A

R

P

N

A

G

-

R

-

L

G

G

F

S

A

V

P

A

I

L

A

A

I

V

G

G

F

F

G

S

L

L

T

T

L

L

I

G

H

H

L

L

V

F

S

G

I

M

P

Y

V

Y

T

T

N

G

T

A

S

G

V

M

N

N

P

P

A

A

R

R

S

S

T

F

G

A

V

P

A

A

L
x
I

F
x
L

A

-

D

-

T

T

A
x
R

A

N

L
x
F

S

T

Q

N

L

H

W

W

L

V

F

Y

W
|
W

V

V

A

G

P

P

L

V

V

I

G

G

A

A

V

G

I

L

G

G

A

S

I

L

W

Y

K

D

G

F

L

L

binding cholesterol: I82 (= I82), F193 (≠ L200), W200 (= W207)
binding [(E,2S,3R)-2-(hexadecanoylamino)-3-oxidanyl-octadec-4-enyl] 2-(trimethylazaniumyl)ethyl phosphate: F12 (= F9), V86 (= V86), V86 (= V86), L89 (≠ V89), L90 (= L90), L90 (= L90), L90 (= L90), Y100 (= Y100), Y100 (= Y100), S101 (≠ V101), I188 (≠ L193), L189 (≠ F194), R191 (≠ A198), F193 (≠ L200)

Sites not aligning to the query:

binding [(E,2S,3R)-2-(hexadecanoylamino)-3-oxidanyl-octadec-4-enyl] 2-(trimethylazaniumyl)ethyl phosphate: 5, 5, 9, 9

8ct2D Local refinement of aqp1 tetramer (c1; refinement mask included d1 of protein 4.2 and ankyrin-1 ar1-5) in class 2 of erythrocyte ankyrin-1 complex (see paper)
38% identity, 96% coverage: 7:225/228 of query aligns to 14:229/247 of 8ct2D

query
sites
8ct2D

A

A

E

E

F

F

F

L

G

A

T

T

F

T

W

L

L

F

V

V

F

F

G

I

G

S

C

I

G

G

S

S

A

A

I

-

L

L

A

G

A

F

A

K

F

Y

P

P

-

V

-

G

-

N

E

Q

L

T

G

A

I

V

-

Q

G

D

F

N

L

V

G

K

V

V

A

S

L

L

A

A

F

F

G

G

L

L

T

S

V

I

L

A

T

T

M

L

A

A

Y

Q

A

S

V

V

G

G

G

H

I

I

S

S

G

G

G

A

H

H

F

L

N

N

P

P

A

A

V

V

S

T

L

L

G

G

L

L

T

L

V

L

A

S

G

C

R

Q

L

I

P

S

A

I

K

F

D

R

L

A

I

L

P

M

Y

Y

W

I

V

I

A

A

Q

Q

V

C

L

V

G

G

A

A

I

I

A

V

A

A

A

T

A

A

I

I

L

L

Y

S

V

G

I

I

A

T

S

S

G

S

K

L

D

T

G

G

F

N

S

S

A

L

G

-

L

-

A

-

S

-

N

-

G

G

Y

R

G

N

E

D

L

L

S

A

P

D

G

G

G

V

Y

N

S

S

M
x
G

M

Q

A

-

G

G

L

L

L
x
G

I

I

E

E

I

I

L

G

T

T

A

L

F

Q

F

L

I

V

I

L

I

C

I

V

L

L

G

A

S

T

T

T

S

D

S

R

L

R

A

R

P

R

A

D

-

L

-

G

G

G

F

S

A

A

P

P

I

L

A

A

I

I

G

G

F

L

G

S

L

V

T

A

L

L

I

G

H

H

L

L

V

L

S

A

I

I

P

D

V

Y

T

T

N

G

T

C

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

F

G

G

V

S

A

A

L

V

F

I

A

-

D

-

T

T

A

H

A

N

L

F

S

S

Q

N

L

H

W

W

L

I

F

F

W

W

V

V

A

G

P

P

L

F

V

I

G

G

A

G

V

A

I

L

G

A

A

V

I

L

I

I

W

Y

K

D

G

F

L

I

L

L

binding cholesterol: G134 (≠ M129), G138 (≠ L134)

Q08733 Aquaporin PIP1-3; AtPIP1;3; Plasma membrane intrinsic protein 1c; PIP1c; Transmembrane protein B; TMP-B from Arabidopsis thaliana (Mouse-ear cress) (see paper)
34% identity, 93% coverage: 7:219/228 of query aligns to 56:271/286 of Q08733

query
sites
Q08733

A

A

E

E

F

F

F

I

G

A

T

T

F

F

W

L

L

F

V

L

F

Y

G

I

G

T

C

V

G

L

S

T

A

V

I

M

L

G

A

V

A

K

A

R

F

A

P

P

E

N

L

M

-

C

-

A

G

S

I

V

G

G

F

I

L

Q

G

G

V

I

A

A

L

W

A

A

F

F

G

G

L

G

T

M

V

I

L

F

T

A

M

L

A

V

Y

Y

A

C

V

T

G

A

G

G

I

I

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

V

S

T

L

F

G

G

L

L

T

F

V

L

A

A

G

R

R

K

L

L

P

S

A

L

K

T

D

R

L

A

I

V

P

F

Y

Y

W

I

V

V

A

M

Q

Q

V

C

L

L

G

G

A

A

I

I

A

C

A

G

A

A

A

G

I

V

L

V

Y

-

V

-

I

-

A

-

S

-

G

-

K

-

D

-

G

-

F

-

S

-

A

-

G

K

G

G

L

F

A

Q

S

P

N

N

G

P

Y

Y

G

Q

E

T

L

L

S

G

P

G

G

G

-

A

-

N

-

T

-

V

-

A

-

H

G

G

Y

Y

S

T

M

K

M

G

A

S

G

G

L

L

L

G

I

A

E

E

I

I

L

G

T

T

A

F

F

V

F

L

I

V

I

Y

I

T

I

V

L

F

G

S

S

A

T

T

S

D

S

A

L

K

A

R

P

S

A

A

G

R

-

D

-

S

-

H

-

V

-

P

-

I

F

L

A

A

P

P

I

L

A

P

I

I

G

G

F

F

G

A

L

V

T

F

L

L

I

V

H

H

L

L

V

A

S

T

I

I

P

P

V

I

T

T

N

G

T

T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

L

G

G

V

A

A

A

-

I

L

I

F

Y

A

N

D

K

T

D

A

H

A

A

L

W

S

D

Q

D

L

H

W

W

L

I

F

F

W

W

V

V

A

G

P

P

L

F

V

I

G

G

A

A

V

A

I

L

G

A

A

A

I

L

Sites not aligning to the query:

1 modified: N-acetylmethionine

P56402 Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct from Mus musculus (Mouse) (see 2 papers)
38% identity, 96% coverage: 7:225/228 of query aligns to 15:223/271 of P56402

query
sites
P56402

A

A

E

E

F

F

F

L

G

A

T

T

F

L

W

L

L

F

V

V

F

F

G

F

G

G

C

L

G

G

S

S

A

A

I

L

L

Q

A

W

A

A

A

S

F

S

P

P

E

P

L

-

G

-

I

-

G

S

F

V

L

L

G

Q

V

I

A

A

L

V

A

A

F

F

G

G

L

L

T

G

V

I

L

G

T

T

M

L

A

V

Y

Q

A

A

V

L

G

G

G

H

I

V

S

S

G

G

G

A

H

H

F

I

N

N

P

P

A

A

V

V

S

T

L

V

G

A

L

C

T

L

V

V

A

G

G

C

R

H

L

V

P

S

A

F

K

L

D

R

L

A

I

A

P

F

Y

Y

W

V

V

A

A

A

Q

Q

V

L

L

L

G

G

A

A

I

V

A

A

A

G

A

A

I

I

L

L

Y

H

V

E

I

I

A

T

S

P

G

V

K

E

D

-

G

-

F

-

S

I

A

R

G

G

G

D

L

L

A

A

S

V

N

N

G

A

Y

L

G

H

E

N

L

N

S

A

P
x
T

G

A

G

G

Y

Q

S

A

M

V

M

T

A

-

G

-

L

-

I

V

E

E

I

L

I

F

L

L

T

T

A

M

F

Q

F

L

I

V

I

L

I

C

I

I

L

F

G

A

S

S

T

T

S

D

S

E

L

R

A

R

P

S

A

D

-

N

-

L

G

G

F

S

A

P

P

A

I

L

A

S

I

I

G

G

F

F

G

S

L

V

T

T

L

L

I

G

H

H

L

L

V

L

S

G

I

I

P

Y

V

F

T

T

N

G

T

C

S

S

V

M

N

N

P

P

A

A

R

R

S

S

T

L

G

A

V

P

A

A

L

V

F

V

A

-

D

-

T

T

A

G

A

K

L

F

S

D

Q

D

L

H

W

W

L

V

F

F

W

W

V

I

A

G

P

P

L

L

V

V

G

G

A

A

V

V

I

I

G

G

A

S

I

L

W

Y

K

N

G

Y

L

L

T126 (≠ P124) mutation to M: Does not cause loss of water channel activity, but impairs trafficking from cytoplasmic vesicles to the cell membrane.

Sites not aligning to the query:

256 modified: Phosphoserine; S → L: in cph; loss of a phosphorylation site and loss of trafficking to the apical cell membrane; causes aberrant location at the basolateral cell membrane

Q06611 Aquaporin PIP1-2; AtPIP1;2; Plasma membrane intrinsic protein 1b; PIP1b; Transmembrane protein A; AthH2; TMP-A from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 93% coverage: 7:219/228 of query aligns to 56:271/286 of Q06611

query
sites
Q06611

A

A

E

E

F

F

F

I

G

A

T

T

F

F

W

L

L

F

V

L

F

Y

G

I

G

T

C

V

G

L

S

T

A

V

I

M

L

G

A

V

A

K

A

R

F

S

P

P

E

N

L

M

-

C

-

A

G

S

I

V

G

G

F

I

L

Q

G

G

V

I

A

A

L

W

A

A

F

F

G

G

L

G

T

M

V

I

L

F

T

A

M

L

A

V

Y

Y

A

C

V

T

G

A

G

G

I

I

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

V

S

T

L

F

G

G

L

L

T

F

V

L

A

A

G

R

R

K

L

L

P

S

A

L

K

T

D

R

L

A

I

V

P

Y

Y

Y

W

I

V

V

A

M

Q

Q

V

C

L

L

G

G

A

A

I

I

A

C

A

G

A

A

A

G

I

V

L

V

Y

-

V

-

I

-

A

-

S

-

G

-

K

-

D

K

G

G

F

F

S

Q

A

P

G

K

G

Q

L

Y

A

Q

S

A

N

L

G

G

Y

G

G

G

E

A

L

N

S

T

-

I

P

A

G

H

G

G

Y

Y

S

T

M

K

M

G

A

S

G

G

L

L

L

G

I

A

E

E

I

I

L

G

T

T

A

F

F

V

F

L

I

V

I

Y

I

T

I

V

L

F

G

S

S

A

T

T

S

D

S

A

L

K

A

R

P

N

A

A

G

R

-

D

-

S

-

H

-

V

-

P

-

I

F

L

A

A

P

P

I

L

A

P

I

I

G

G

F

F

G

A

L

V

T

F

L

L

I

V

H

H

L

L

V

A

S

T

I

I

P

P

V

I

T

T

N

G

T

T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

L

G

G

V

A

A

A

-

I

L

I

F

F

A

N

D

K

T

D

A

N

A

A

L

W

S

D

Q

D

L

H

W

W

L

V

F

F

W

W

V

V

A

G

P

P

L

F

V

I

G

G

A

A

V

A

I

L

G

A

A

A

I

L

Sites not aligning to the query:

1 modified: N-acetylmethionine

P30302 Aquaporin PIP2-3; Plasma membrane intrinsic protein 2-3; AtPIP2;3; Plasma membrane intrinsic protein 2c; PIP2c; RD28-PIP; TMP2C; Water stress-induced tonoplast intrinsic protein; WSI-TIP from Arabidopsis thaliana (Mouse-ear cress) (see paper)
36% identity, 85% coverage: 33:225/228 of query aligns to 75:268/285 of P30302

query
sites
P30302

G

G

I

V

G

G

F

I

L

L

G

G

V

I

A

A

L

W

A

A

F

F

G

G

L

G

T

M

V

I

L

F

T

I

M

L

A

V

Y

Y

A

C

V

T

G

A

G

G

I

I

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

V

S

T

L

F

G

G

L

L

T

F

V

L

A

A

G

R

R

K

L

V

P

S

A

L

K

I

D

R

L

A

I

V

P

L

Y

Y

W

M

V

V

A

A

Q

Q

V

C

L

L

G

G

A

A

I

I

A

C

A

G

A

V

A

G

I

F

L

V

Y

K

V

A

I

F

A

Q

S

S

G

S

K

H

D

Y

G

V

F

N

S

Y

A

G

G

G

L

-

A

-

S

-

N

-

G

-

Y

-

G

A

E

N

L

F

S

L

P

A

G

D

G

G

Y

Y

S

N

M

T

M

G

A

T

G

G

L

L

L

A

I

A

E

E

I

I

L

G

T

T

A

F

F

V

F

L

I

V

I

Y

I

T

I

V

L

F

G

S

S

A

T

T

S

D

S

P

L

K

A

R

P

N

A

A

G

R

-

D

-

S

-

H

-

V

-

P

-

V

F

L

A

A

P

P

I

L

A

P

I

I

G

G

F

F

G

A

L

V

T

F

L

M

I

V

H

H

L

L

V

A

S

T

I

I

P

P

V

I

T

T

N

G

T
|
T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

F

G

G

V

A

A

A

-

V

L

I

F

F

A

N

D

K

T

S

A

K

A

P

L

W

S

D

Q

D

L

H

W

W

L

I

F

F

W

W

V

V

A

G

P

P

L

F

V

I

G

G

A

A

V

T

I

I

G

A

A

A

I

F

I

Y

W

H

K

Q

G

F

L

V

L

L

T223 (= T181) mutation to C: Creates some mercury-sensitivity.

P43287 Aquaporin PIP2-2; Plasma membrane intrinsic protein 2-2; AtPIP2;2; Plasma membrane intrinsic protein 2b; PIP2b; TMP2b from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
36% identity, 85% coverage: 33:225/228 of query aligns to 75:268/285 of P43287

query
sites
P43287

G

G

I

V

G

G

F

I

L

L

G

G

V

I

A

A

L

W

A

A

F

F

G

G

L

G

T

M

V

I

L

F

T

I

M

L

A

V

Y

Y

A

C

V

T

G

A

G

G

I

I

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

V

S

T

L

F

G

G

L

L

T

F

V

L

A

A

G

R

R

K

L

V

P

S

A

L

K

I

D

R

L

A

I

V

P

L

Y

Y

W

M

V

V

A

A

Q

Q

V

C

L

L

G

G

A

A

I

I

A

C

A

G

A

V

A

G

I

F

L

V

Y

K

V

A

I

F

A

Q

S

S

G

S

K

Y

D

Y

G

D

F

R

S

Y

A

G

G

G

L

-

A

-

S

A

N

N

G

S

Y

L

G

A

E

D

L

-

S

-

P

-

G

-

G

G

Y

Y

S

N

M

T

M

G

A

T

G

G

L

L

L

A

I

A

E

E

I

I

L

G

T

T

A

F

F

V

F

L

I

V

I

Y

I

T

I

V

L

F

G

S

S

A

T

T

S

D

S

P

L

K

A

R

P

N

A

A

G
x
R

-
x
D

-

S

-
x
H

-

V

-

P

-

V

F

L

A

A

P

P

I

L

A

P

I

I

G

G

F

F

G

A

L

V

T

F

L

M

I

V

H

H

L

L

V

A

S

T

I

I

P

P

V

I

T

T

N

G

T

T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

F

G

G

V

A

A

A

-

V

L

I

F

Y

A

N

D

K

T

S

A

K

A

P

L

W

S

D

Q

D

L

H

W

W

L

I

F

F

W

W

V

V

A

G

P

P

L

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G

G

A

A

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A

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A

A

A

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Y

W
x
H

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G

F

L

V

L

L

R194 (≠ G158) mutation to A: Reduced sensitivity to cytosolic acidification. Insensitive to cytosolic acidification; when associated with A-197.
D195 (vs. gap) mutation to A: Reduced sensitivity to cytosolic acidification. Insensitive to cytosolic acidification; when associated with A-197.
H197 (vs. gap) mutation to A: Reduced water transport activity. Reduced sensitivity to cytosolic acidification. Insensitive to cytosolic acidification; when associated with A-194 or A-195.; mutation to D: Reduced water transport activity. Insensitive to cytosolic acidification.; mutation to K: Very low water transport activity. Insensitive to cytosolic acidification.
H264 (≠ W221) mutation to A: No effect.

Sites not aligning to the query:

3 modified: N6,N6-dimethyllysine; partial

P43286 Aquaporin PIP2-1; Plasma membrane intrinsic protein 2-1; AtPIP2;1; Plasma membrane intrinsic protein 2a; PIP2a from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
36% identity, 85% coverage: 33:225/228 of query aligns to 77:270/287 of P43286

query
sites
P43286

G

G

I

V

G

G

F

I

L

L

G

G

V

I

A

A

L

W

A

A

F

F

G

G

L

G

T

M

V

I

L

F

T

I

M

L

A

V

Y

Y

A

C

V

T

G

A

G

G

I

I

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

V

S

T

L

F

G

G

L

L

T

F

V

L

A

A

G

R

R

K

L

V

P

S

A

L

K

P

D

R

L

A

I

L

P

L

Y

Y

W

I

V

I

A

A

Q

Q

V

C

L

L

G

G

A

A

I

I

A

C

A

G

A

V

A

G

I

-

L

-

Y

F

V

V

I

K

A

A

S

F

G

Q

K

S

D

S

G

Y

F

Y

S

T

A

R

G

Y

G

G

L

G

A

G

S

A

N

N

G

S

Y

L

G

A

E

D

L

-

S

-

P

-

G

-

G

G

Y

Y

S

S

M

T

M

G

A

T

G

G

L

L

L

A

I

A

E

E

I

I

L

G

T

T

A

F

F

V

F

L

I

V

I

Y

I

T

I

V

L

F

G

S

S

A

T

T

S

D

S

P

L

K

A

R

P

S

A

A

G

R

-

D

-

S

-

H

-

V

-

P

-

V

F

L

A

A

P

P

I

L

A

P

I

I

G

G

F

F

G

A

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F

L

M

I

V

H

H

L

L

V

A

S

T

I

I

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P

V

I

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T

N

G

T

T

S

G

V

I

N

N

P

P

A

A

R

R

S

S

T

F

G

G

V

A

A

A

-

V

L

I

F

Y

A

N

D

K

T

S

A

K

A

P

L

W

S

D

Q

D

L

H

W

W

L

I

F

F

W

W

V

V

A

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P

P

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F

V

I

G

G

A

A

V

A

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I

G

A

A

A

I

F

I

Y

W

H

K

Q

G

F

L

V

L

L

Sites not aligning to the query:

3 modified: N6,N6-dimethyllysine; partial; K→A: 2-fold decrease in water transport activity.; K→R: No effect.
6 E→A: No effect.
280 modified: Phosphoserine; S→A: Normal subcellular localization.
283 modified: Phosphoserine; S→A: Intracellular reticulation pattern, probably corresponding to the endoplasmic reticulum.; S→D: Normal subcellular localization.

P61837 Aquaporin PIP1-1; AtPIP1;1; Plasma membrane aquaporin-1; Plasma membrane intrinsic protein 1a; PIP1a from Arabidopsis thaliana (Mouse-ear cress) (see paper)
34% identity, 93% coverage: 7:219/228 of query aligns to 56:271/286 of P61837

query
sites
P61837

A

A

E

E

F

F

F

I

G

A

T

T

F

F

W

L

L

F

V

L

F

Y

G

I

G

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C

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G

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S

T

A

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A

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A

K

A

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S

P

P

E

N

L

M

-

C

-

A

G

S

I

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G

G

F

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Q

G

G

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A

A

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A

A

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F

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G

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M

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A

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Y

Y

A

C

V

T

G

A

G

G

I

I

S

S

G

G

H

H

F

I

N

N

P

P

A

A

V

V

S

T

L

F

G

G

L

L

T

F

V

L

A

A

G

R

R

K

L

L

P

S

A

L

K

T

D

R

L

A

I

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P

Y

Y

Y

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I

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V

A

M

Q

Q

V

C

L

L

G

G

A

A

I

I

A

C

A

G

A

A

A

G

I

V

L

V

Y

-

V

-

I

-

A

-

S

-

G

-

K

-

D

K

G

G

F

F

S

Q

A

P

G

K

G

Q

L

Y

A

Q

S

A

N

L

G

G

Y

G

G

G

-

A

E

N

L

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S

V

P

A

G

H

G

G

Y

Y

S

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M

G

A

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G

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I

A

E

E

I

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A

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F

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F

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F

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A

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S

A

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A

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N

A

A

G

R

-

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-

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I

F

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A

A

P

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G

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N

P

P

A

A

R

R

S

S

T

L

G

G

V

A

A

A

-

I

L

I

F

Y

A

N

D

K

T

D

A

H

A

S

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S

D

Q

D

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W

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G

G

A

A

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A

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G

A

A

A

I

L

Sites not aligning to the query:

1 modified: N-acetylmethionine

Query Sequence

>WP_002965064.1 NCBI__GCF_000182725.1:WP_002965064.1
MLNKLSAEFFGTFWLVFGGCGSAILAAAFPELGIGFLGVALAFGLTVLTMAYAVGGISGG
HFNPAVSLGLTVAGRLPAKDLIPYWVAQVLGAIAAAAILYVIASGKDGFSAGGLASNGYG
ELSPGGYSMMAGLLIEIILTAFFIIIILGSTSSLAPAGFAPIAIGFGLTLIHLVSIPVTN
TSVNPARSTGVALFADTAALSQLWLFWVAPLVGAVIGAIIWKGLLGRD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory