SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_002965962.1 NCBI__GCF_000022745.1:WP_002965962.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
40% identity, 94% coverage: 4:239/252 of query aligns to 14:251/265 of P07821

query
sites
P07821

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K50 (= K40) mutation to Q: Lack of activity.
D172 (= D160) mutation to E: Lack of activity.
E173 (= E161) mutation to A: Lack of activity.

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
31% identity, 88% coverage: 1:223/252 of query aligns to 17:235/378 of P69874

query
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P69874

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C26 (≠ S10) mutation to A: Lower ATPase activity and transport efficiency.
F27 (≠ Y11) mutation to L: Lower ATPase activity and transport efficiency.
F45 (≠ I29) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ A38) mutation to T: Loss of ATPase activity and transport.
L60 (= L44) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ V60) mutation to P: Lower ATPase activity and transport efficiency.
V135 (≠ L123) mutation to M: Loss of ATPase activity and transport.
D172 (= D160) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

3fvqB Crystal structure of the nucleotide binding domain fbpc complexed with atp (see paper)
35% identity, 88% coverage: 2:223/252 of query aligns to 4:225/350 of 3fvqB

query
sites
3fvqB

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binding adenosine-5'-triphosphate: F13 (≠ Y11), Q14 (≠ N12), T16 (= T14), V18 (= V16), S38 (≠ N36), G39 (= G37), C40 (≠ A38), G41 (= G39), K42 (= K40), T43 (≠ S41), T44 (= T42), R133 (= R131), E137 (= E135), S139 (= S137), G141 (= G139), Q142 (= Q140)
binding calcium ion: T43 (≠ S41), Q86 (= Q82)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
30% identity, 89% coverage: 1:225/252 of query aligns to 1:225/240 of 4ymuJ

query
sites
4ymuJ

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binding adenosine-5'-triphosphate: F11 (≠ Y11), V16 (= V16), S36 (≠ N36), G37 (= G37), S38 (≠ A38), G39 (= G39), K40 (= K40), S41 (= S41), T42 (= T42), E162 (= E161), H194 (= H194)
binding magnesium ion: S41 (= S41), E162 (= E161)

3c4jA Abc protein artp in complex with atp-gamma-s
27% identity, 89% coverage: 1:224/252 of query aligns to 3:226/242 of 3c4jA

query
sites
3c4jA

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binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y11), V18 (= V16), S38 (≠ N36), G39 (= G37), S40 (≠ A38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42)

3c41J Abc protein artp in complex with amp-pnp/mg2+
27% identity, 89% coverage: 1:224/252 of query aligns to 3:226/242 of 3c41J

query
sites
3c41J

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L

I

P

R

A

E

R

G

G

E

I

V

T

V

S

V

I

V

I

I

G

G

P

P

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

S

R

M

C

V

L

S

N

R

L

L

L

L

E

P

D

M

F

D

D

K

E

G

G

S

E

V

I

T

I

V

I

D

D

G

G

L

I

D

N

I

L

T

K

T

A

-

K

-

D

T

T

S

N

G

L

D

N

V

K

L

V

A

R

R

E

L

V

S

G

I

M

L

V

R

F

Q

Q

E

R

N

F

A

N

I

L

N

F

S

P

R

H

L

M

T

T

V

V

R

L

D

N

L

N

V

I

T

T

F

L

G

A

R

P

Y

M

P

K

Y

V

S

R

K

K

G

W

R

-

P

P

N

R

S

E

E

K

D

A

R

E

K

A

H

K

V

A

E

M

Q

E

A

L

I

L

G

D

Y

K

L

V

G

G

L

L

E

K

G

D

L

K

S

A

E

H

R

A

F

Y

L

P

D

D

E

S

L

L

S

S

G

G

Q

Q

R

A

Q

Q

R

R

A

V

F

A

V

I

A

A

M

R

V

A

L

L

C

A

Q

M

D

E

T

P

D

K

Y

I

V

M

L

L

L

F

D
|
D

E

E

P

P

L

T

N

S

N

A

L

L

D

D

M

P

K

E

H

M

S

V

A

G

S

E

M

V

M

L

K

S

L

V

L

M

R

K

R

Q

A

L

A

A

D

N

E

E

L

-

G

G

K

M

T

T

I

M

V

V

L

V

V

V

L

T

H

H

D

E

I

M

N

G

F

F

A

A

S

R

C

E

Y

V

S

G

D

D

H

R

I

V

V

L

A

F

M

M

R

D

H

G

G

G

K

Y

V

I

H

E

Q

E

G

G

S

K

P

P

D

E

E

D

L

L

I

F

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y11), V18 (= V16), S38 (≠ N36), G39 (= G37), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42)
binding magnesium ion: S43 (= S41), D163 (= D160)

2olkA Abc protein artp in complex with adp-beta-s
27% identity, 89% coverage: 1:224/252 of query aligns to 3:226/242 of 2olkA

query
sites
2olkA

M

M

I

I

E

D

I

V

S

H

Q

Q

V

L

S

K

K

K

S

S

Y
x
F

N

G

G

S

T

L

L

E

V
|
V

V

L

D

K

D

G

V

I

T

N

L

V

R

H

L

I

P

R

A

E

R

G

G

E

I

V

T

V

S

V

I

V

I

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

S

R

M

C

V

L

S

N

R

L

L

L

L

E

P

D

M

F

D

D

K

E

G

G

S

E

V

I

T

I

V

I

D

D

G

G

L

I

D

N

I

L

T

K

T

A

-

K

-

D

T

T

S

N

G

L

D

N

V

K

L

V

A

R

R

E

L

V

S

G

I

M

L

V

R

F

Q

Q

E

R

N

F

A

N

I

L

N

F

S

P

R

H

L

M

T

T

V

V

R

L

D

N

L

N

V

I

T

T

F

L

G

A

R

P

Y

M

P

K

Y

V

S

R

K

K

G

W

R

-

P

P

N

R

S

E

E

K

D

A

R

E

K

A

H

K

V

A

E

M

Q

E

A

L

I

L

G

D

Y

K

L

V

G

G

L

L

E

K

G

D

L

K

S

A

E

H

R

A

F

Y

L

P

D

D

E

S

L

L

S

S

G

G

Q

Q

R

A

Q

Q

R

R

A

V

F

A

V

I

A

A

M

R

V

A

L

L

C

A

Q

M

D

E

T

P

D

K

Y

I

V

M

L

L

L

F

D

D

E

E

P

P

L

T

N

S

N

A

L

L

D

D

M

P

K

E

H

M

S

V

A

G

S

E

M

V

M

L

K

S

L

V

L

M

R

K

R

Q

A

L

A

A

D

N

E

E

L

-

G

G

K

M

T

T

I

M

V

V

L

V

V

V

L

T

H

H

D

E

I

M

N

G

F

F

A

A

S

R

C

E

Y

V

S

G

D

D

H

R

I

V

V

L

A

F

M

M

R

D

H

G

G

G

K

Y

V

I

H

E

Q

E

G

G

S

K

P

P

D

E

E

D

L

L

I

F

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y11), V18 (= V16), S38 (≠ N36), G39 (= G37), S40 (≠ A38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42)

2oljA Abc protein artp in complex with adp/mg2+
27% identity, 89% coverage: 1:224/252 of query aligns to 3:226/242 of 2oljA

query
sites
2oljA

M

M

I

I

E

D

I

V

S

H

Q

Q

V

L

S

K

K

K

S

S

Y
x
F

N

G

G

S

T

L

L

E

V
|
V

V

L

D

K

D

G

V

I

T

N

L

V

R

H

L

I

P

R

A

E

R

G

G

E

I

V

T

V

S

V

I

V

I

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

S

R

M

C

V

L

S

N

R

L

L

L

L

E

P

D

M

F

D

D

K

E

G

G

S

E

V

I

T

I

V

I

D

D

G

G

L

I

D

N

I

L

T

K

T

A

-

K

-

D

T

T

S

N

G

L

D

N

V

K

L

V

A

R

R

E

L

V

S

G

I

M

L

V

R

F

Q

Q

E

R

N

F

A

N

I

L

N

F

S

P

R

H

L

M

T

T

V

V

R

L

D

N

L

N

V

I

T

T

F

L

G

A

R

P

Y

M

P

K

Y

V

S

R

K

K

G

W

R

-

P

P

N

R

S

E

E

K

D

A

R

E

K

A

H

K

V

A

E

M

Q

E

A

L

I

L

G

D

Y

K

L

V

G

G

L

L

E

K

G

D

L

K

S

A

E

H

R

A

F

Y

L

P

D

D

E

S

L

L

S

S

G

G

Q

Q

R

A

Q

Q

R

R

A

V

F

A

V

I

A

A

M

R

V

A

L

L

C

A

Q

M

D

E

T

P

D

K

Y

I

V

M

L

L

L

F

D

D

E

E

P

P

L

T

N

S

N

A

L

L

D

D

M

P

K

E

H

M

S

V

A

G

S

E

M

V

M

L

K

S

L

V

L

M

R

K

R

Q

A

L

A

A

D

N

E

E

L

-

G

G

K

M

T

T

I

M

V

V

L

V

V

V

L

T

H

H

D

E

I

M

N

G

F

F

A

A

S

R

C

E

Y

V

S

G

D

D

H

R

I

V

V

L

A

F

M

M

R

D

H

G

G

G

K

Y

V

I

H

E

Q

E

G

G

S

K

P

P

D

E

E

D

L

L

I

F

binding adenosine-5'-diphosphate: F13 (≠ Y11), V18 (= V16), S38 (≠ N36), G39 (= G37), S40 (≠ A38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42)

7chaI Cryo-em structure of p.Aeruginosa mlafebd with amppnp (see paper)
30% identity, 88% coverage: 2:223/252 of query aligns to 4:227/262 of 7chaI

query
sites
7chaI

I

V

E

E

I

L

S

K

Q

G

V

L

S

T

K

F

S

K

Y
x
R

N

G

G

S

T

R

L

A

V

I

V

F

D

D

D

N

V

I

T

D

L

V

R

R

L

I

P

P

A

R

R

G

G

K

I

V

T

T

S

G

I

I

I

M

G

G

P

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
x
T

T
|
T

L

L

S

R

M

L

V

I

S

A

R

S

L

Q

L

L

P

R

M

P

D

S

K

K

G

G

S

E

V

V

T

W

V

V

D

N

G

G

L

Q

D

N

I

L

T

P

T

Q

T

L

S

S

-

R

G

G

D

D

V

L

-

F

-

D

L

M

A

R

R

K

R

Q

L

F

S

G

I

V

L

L

R

F

Q

Q

E

S

N

G

A

A

I

L

N

F

S

T

R

D

L

L

T

D

V

V

R

F

D

E

L

N

V

V

T

A

F

F

G

P

R

L

Y

R

P

V

Y

H

S

T

K

Q

G

-

R

L

P

P

N

E

S

E

E

M

D

I

R

R

K

D

H

I

V

V

E

L

Q

M

A

K

I

L

G

Q

Y

A

L

V

G

G

L

L

E

R

G

G

L

A

S

V

E

E

R

L

F

M

L

P

D

D

E

E

L

L

S

S

G

G

Q

M

R

K

Q

R

R

R

A

V

F

A

V

L

A

A

M

R

V

A

L

I

C

A

Q

L

D

D

T

P

D

Q

Y

I

V

L

L

L

L

Y

D

D

E

E

P

P

L

F

N

V

N

G

L

Q

D

D

M

P

K

I

H

A

S

M

A

G

S

V

M

L

M

V

K

R

L

L

L

I

R

R

R

L

A

L

A

N

D

D

E

A

L

L

G

G

K

I

T

T

I

S

V

I

L

V

V

V

L

S

H

H

D

D

I

L

N

A

F

E

A

T

S

A

C

S

Y

I

S

A

D

D

H

Y

I

I

V

Y

A

I

M

V

R

G

H

D

G

G

K

R

V

V

V

L

H

G

Q

H

G

G

S

T

P

P

D

D

E

V

L

L

binding phosphoaminophosphonic acid-adenylate ester: R13 (≠ Y11), S38 (≠ N36), G39 (= G37), C40 (≠ A38), G41 (= G39), K42 (= K40), T43 (≠ S41), T44 (= T42)

8hplC Lpqy-sugabc in state 1 (see paper)
32% identity, 88% coverage: 2:223/252 of query aligns to 3:219/384 of 8hplC

query
sites
8hplC

I

I

E

V

I

L

S

D

Q

R

V

V

S

T

K

K

S

S

Y
|
Y

N

P

G

R

T

A

L

-

V

A

V

V

D

K

D

E

V

F

T

S

L

M

R

T

L

I

P

A

A

D

R

G

G

E

I

F

T

I

S

I

I

L

I

V

G

G

P

P

N

S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

T

L

L

S

N

M

M

V

I

S

A

R

G

L

L

L

E

P

E

M

I

D

T

K

S

G

G

S

E

V

L

T

R

V

I

D

G

G

G

L

E

D

R

I

V

T

N

T

E

T

K

S

A

G

P

D

K

V

-

L

-

A

D

R

R

R

D

L

I

S

A

I

M

L

V

R

F

Q

Q

E

S

N

Y

A

A

I

L

N

Y

S

P

R

H

L

M

T

T

V

V

R

R

D

Q

L

N

V

I

T

A

F

F

G

-

R

-

Y

-

P

P

Y

L

S

T

K

L

G

A

R

K

-

V

P

P

N

K

S

A

E

E

D

I

R

A

K

A

H

K

V

V

E

E

Q

E

A

T

I

A

G

K

Y

I

L

L

G

D

L

L

E

S

G

E

L

L

S

L

E

D

R

R

F

K

L

P

D

G

E

Q

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

A

V

F

A

V

M

A

G

M

R

V

A

L

I

C

V

Q

R

D

S

T

P

D

K

Y

A

V

F

L

L

L

M

D

D

E

Q

P

P

L

L

N

S

N

N

L

L

D

D

M

A

K

K

H

L

S

R

A

V

S

Q

M

M

M

R

K

A

L

E

L

I

R

S

R

R

A

L

A

Q

D

D

E

R

L

L

G

G

K

T

T

T

I

T

V

V

L

Y

V

V

L

T

H

H

D

D

I

Q

N

T

F

E

A

A

S

M

C

T

Y

L

S

G

D

D

H

R

I

V

V

V

A

V

M

M

R

L

H

A

G

G

K

E

V

V

V

Q

H

Q

Q

I

G

G

S

T

P

P

D

D

E

E

L

L

binding adenosine-5'-triphosphate: Y12 (= Y11), G37 (= G37), C38 (≠ A38), G39 (= G39), K40 (= K40), S41 (= S41), T42 (= T42)

8hprD Lpqy-sugabc in state 4 (see paper)
32% identity, 88% coverage: 2:223/252 of query aligns to 3:221/362 of 8hprD

query
sites
8hprD

I

I

E

V

I

L

S

D

Q

R

V

V

S

T

K

K

S

S

Y
|
Y

N

P

G

D

T

V

-

R

L

A

V

A

V

V

D

K

D

E

V

F

T

S

L

M

R

T

L

I

P

A

A

D

R

G

G

E

I

F

T

I

S

I

I

L

I

V

G

G

P

P

N
x
S

G

G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

T

L

L

S

N

M

M

V

I

S

A

R

G

L

L

L

E

P

E

M

I

D

T

K

S

G

G

S

E

V

L

T

R

V

I

D

G

G

G

L

E

D

R

I

V

T

N

T

E

T

K

S

A

G

P

D

K

V

-

L

-

A

D

R

R

R

D

L

I

S

A

I

M

L

V

R

F

Q
|
Q

E

S

N

Y

A

A

I

L

N

Y

S

P

R

H

L

M

T

T

V

V

R

R

D

Q

L

N

V

I

T

A

F

F

G

-

R

-

Y

-

P

P

Y

L

S

T

K

L

G

A

R

K

-

V

P

P

N

K

S

A

E

E

D

I

R

A

K

A

H

K

V

V

E

E

Q

E

A

T

I

A

G

K

Y

I

L

L

G

D

L

L

E

S

G

E

L

L

S

L

E

D

R
|
R

F

K

L

P

D

G

E
x
Q

L

L

S
|
S

G
|
G

Q

Q

R

R

Q

Q

R

R

A

V

F

A

V

M

A

G

M

R

V

A

L

I

C

V

Q

R

D

S

T

P

D

K

Y

A

V

F

L

L

L

M

D

D

E
x
Q

P

P

L

L

N

S

N

N

L

L

D

D

M

A

K

K

H

L

S

R

A

V

S

Q

M

M

M

R

K

A

L

E

L

I

R

S

R

R

A

L

A

Q

D

D

E

R

L

L

G

G

K

T

T

T

I

T

V

V

L

Y

V

V

L

T

H
|
H

D

D

I

Q

N

T

F

E

A

A

S

M

C

T

Y

L

S

G

D

D

H

R

I

V

V

V

A

V

M

M

R

L

H

A

G

G

K

E

V

V

V

Q

H

Q

Q

I

G

G

S

T

P

P

D

D

E

E

L

L

binding adenosine-5'-triphosphate: Y12 (= Y11), S38 (≠ N36), C40 (≠ A38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42), Q82 (= Q82), R129 (= R131), Q133 (≠ E135), S135 (= S137), G136 (= G138), G137 (= G139), Q159 (≠ E161), H192 (= H194)
binding magnesium ion: S43 (= S41), Q82 (= Q82)

8hprC Lpqy-sugabc in state 4 (see paper)
32% identity, 88% coverage: 2:223/252 of query aligns to 3:221/363 of 8hprC

query
sites
8hprC

I

I

E

V

I

L

S

D

Q

R

V

V

S

T

K

K

S

S

Y
|
Y

N

P

G

D

T

V

-

R

L

A

V

A

V

V

D

K

D

E

V

F

T

S

L

M

R

T

L

I

P

A

A

D

R

G

G

E

I

F

T

I

S

I

I

L

I

V

G

G

P

P

N
x
S

G
|
G

A

C

G
|
G

K
|
K

S
|
S

T

T

L

T

L

L

S

N

M

M

V

I

S

A

R

G

L

L

L

E

P

E

M

I

D

T

K

S

G

G

S

E

V

L

T

R

V

I

D

G

G

G

L

E

D

R

I

V

T

N

T

E

T

K

S

A

G

P

D

K

V

-

L

-

A

D

R

R

R

D

L

I

S

A

I

M

L

V

R

F

Q
|
Q

E

S

N

Y

A

A

I

L

N

Y

S

P

R

H

L

M

T

T

V

V

R

R

D

Q

L

N

V

I

T

A

F

F

G

-

R

-

Y

-

P

P

Y

L

S

T

K

L

G

A

R

K

-

V

P

P

N

K

S

A

E

E

D

I

R

A

K

A

H

K

V

V

E

E

Q

E

A

T

I

A

G

K

Y

I

L

L

G

D

L

L

E

S

G

E

L

L

S

L

E

D

R

R

F

K

L

P

D

G

E
x
Q

L

L

S

S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

A

V

F

A

V

M

A

G

M

R

V

A

L

I

C

V

Q

R

D

S

T

P

D

K

Y

A

V

F

L

L

L

M

D

D

E

Q

P

P

L

L

N

S

N

N

L

L

D

D

M

A

K

K

H

L

S

R

A

V

S

Q

M

M

M

R

K

A

L

E

L

I

R

S

R

R

A

L

A

Q

D

D

E

R

L

L

G

G

K

T

T

T

I

T

V

V

L

Y

V

V

L

T

H
|
H

D

D

I

Q

N

T

F

E

A

A

S

M

C

T

Y

L

S

G

D

D

H

R

I

V

V

V

A

V

M

M

R

L

H

A

G

G

K

E

V

V

V

Q

H

Q

Q

I

G

G

S

T

P

P

D

D

E

E

L

L

binding adenosine-5'-triphosphate: Y12 (= Y11), S38 (≠ N36), G39 (= G37), G41 (= G39), K42 (= K40), S43 (= S41), Q82 (= Q82), Q133 (≠ E135), G136 (= G138), G137 (= G139), Q138 (= Q140), H192 (= H194)
binding magnesium ion: S43 (= S41), Q82 (= Q82)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
31% identity, 85% coverage: 1:214/252 of query aligns to 1:223/232 of 1f3oA

query
sites
1f3oA

M

M

I

I

E

K

I

L

S

K

Q

N

V

V

S

T

K

K

S

T

Y
|
Y

N

K

-

M

G

G

T

E

L

E

V

I

-

Y

-

A

-

L

D

K

D

N

V

V

T

N

L

L

R

N

L

I

P

K

A

E

R

G

G

E

I

F

T

V

S

S

I

I

I

M

G

G

P

P

N

S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

S

N

M

I

V

I

S

G

R

C

L

L

L

D

P

K

M

P

D

T

K

E

G

G

S

E

V

V

T

Y

V

I

D

D

G

N

L

I

D

K

I

T

T

N

T

D

T

L

S

D

G

D

D

D

V

E

L

L

A

T

R

K

-

I

-

R

-

D

R

K

L

I

S

G

I

F

L

V

R

F

Q

Q

E

Q

N

F

A

N

I

L

N

I

S

P

R

L

L

L

T

T

V

A

R

L

D

E

L

N

V

V

T

E

F

L

G

P

R

L

Y

I

P

F

Y

K

S

Y

K

R

G

G

R

A

P

M

N

S

S

G

E

E

D

E

R

R

K

R

H

-

V

-

E

K

Q

R

A

A

I

L

G

E

Y

C

L

L

G

K

L

M

E

A

G

E

L

L

S

E

E

E

R

R

F

F

L

A

D

N

-

H

-

K

-

P

-

N

E

Q

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

A

V

F

A

V

I

A

A

M

R

V

A

L

L

C

A

Q

N

D

N

T

P

D

P

Y

I

V

I

L

L

L

A

D

D

E

E

P

P

L

T

N

G

N

A

L

L

D

D

M

S

K

K

H

T

S

G

A

E

S

K

M

I

M

M

K

Q

L

L

R

K

A

L

A

N

D

E

E

E

L

D

G

G

K

K

T

T

I

V

V

V

L

V

V

V

L

T

H

H

D

D

I

I

N

N

F

V

A

A

S

R

C

-

Y

F

S

G

D

E

H

R

I

I

V

I

A

Y

M

L

R

K

H

D

G

G

K

E

V

V

binding adenosine-5'-diphosphate: Y11 (= Y11), G41 (= G37), G43 (= G39), K44 (= K40), S45 (= S41), T46 (= T42)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
31% identity, 90% coverage: 1:227/252 of query aligns to 1:233/343 of P30750

query
sites
P30750

M

M

I

I

E

K

I

L

S

S

Q

N

V

I

S

T

K

K

S

V

Y

F

N

H

-

Q

G

G

T

T

L

R

V

T

V

I

-

Q

-

A

-

L

D

N

V

V

T

S

L

L

R

H

L

V

P

P

A

A

R

G

G

Q

I

I

T

Y

S

G

I

V

I

I

G

G

P

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

S

R

M

C

V

V

S

N

R

L

L

L

L

E

P

R

M

P

D

T

K

E

G

G

S

S

V

V

T

L

V

V

D

D

G

G

L

Q

D

E

I

L

T

T

T

L

S

S

G

E

D

S

V

E

L

L

-

T

-

K

A

A

R

R

L

Q

-

I

-

G

S

M

I

I

L

F

R

Q

Q

H

E

F

N

N

A

L

I

L

N

S

S

S

R

R

L

-

T

-

V

-

R

-

D

-

L

-

V

T

T

V

F

F

G

G

R

N

Y

V

P

A

Y

L

S

P

-

L

-

E

-

L

K

D

G

N

R

T

P

P

N

K

S

D

E

E

D

V

R

K

K

R

H

R

V

V

E

T

Q

E

A

L

I

L

G

S

Y

L

L

V

G

G

L

L

E

G

G

D

L

K

S

H

E

D

R

S

F

Y

L

P

D

S

E

N

L

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

A

V

F

A

V

I

A

A

M

R

V

A

L

L

C

A

Q

S

D

N

T

P

D

K

Y

V

V

L

L

L

L

C

D

D

E
|
E

P

A

L

T

N

S

N

A

L

L

D

D

M

P

K

A

H

T

S

T

A

R

S

S

M

I

M

L

K

E

L

L

R

K

R

D

A

I

A

N

D

R

E

R

L

L

G

G

K

L

T

T

I

I

V

L

L

L

V

I

L

T

H

H

D

E

I

M

N

D

F

V

A

V

S

K

C

R

Y

I

S

C

D

D

H

C

I

V

V

A

A

V

M

I

R

S

H

N

G

G

K

E

V

L

V

I

H

E

Q

Q

G

D

S

T

P

V

D

S

E

E

L

V

I

F

-

S

H

H

P

P

E

K

40:46 (vs. 36:42, 86% identical) binding ATP
E166 (= E161) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

5x40A Structure of a cbio dimer bound with amppcp (see paper)
32% identity, 85% coverage: 12:224/252 of query aligns to 16:228/280 of 5x40A

query
sites
5x40A

N

G

G

G

T
x
V

L

K

V
x
A

V

L

D

D

V

L

T

S

L

L

R

A

L

V

P

P

A

K

R

G

G

E

I

S

T

L

S

A

I

I

I

L

G

G

P

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

S

L

M

H

V

L

S

N

R

G

L

T

L

L

P

R

M

P

D

Q

K

S

G

G

S

R

V

V

T

L

V

L

D

G

G

G

L

T

D

A

I

T

T

G

T

H

T

S

S

R

G

K

D

D

V

L

L

T

A

G

-

W

-

R

R

R

L

V

S

G

I

L

L

V

R

L

Q
|
Q

E

D

N

A

A

-

I

-

N

D

S

D

R

Q

L

L

-

F

-

A

-

T

T

T

V

V

R

F

D

E

L

D

V

V

T

S

F

F

G

G

R

-

Y

-

P

P

Y

L

S

N

K

L

G

G

R

L

P

S

N

E

S

A

E

E

D

A

R

R

K

A

H

R

V

V

E

E

Q

E

A

A

I

L

G

A

Y

A

L

L

G

S

L

I

E

S

G

D

L

L

S

R

E

D

R

R

F

P

L

T

D
x
H

E
x
M

L
|
L

S
|
S

G

G

G
|
G

Q
|
Q

R

K

Q

R

R

R

A

V

F

A

V

I

A

A

M

G

V

A

L

V

C

A

Q

M

D

R

T

P

D

E

Y

V

V

L

L

L

D

D

E
x
Q

P

P

L

T

N

A

N

G

L

L

D

D

M

L

K

A

H

G

S

T

A

E

S

Q

M

L

K

T

L

L

R

R

-

G

-

L

R

R

A

A

D

-

E

-

L

-

G

G

K

M

T

T

I

L

V

V

L

F

V

S

L

T

H
|
H

D

D

I

V

N

E

F

L

A

A

S

A

C

A

Y

L

S

A

D

D

H

R

I

V

V

A

A

L

M

F

R

R

H

T

G

G

K

R

V

V

V

L

H

A

Q

E

G

G

S

A

P

A

D

E

E

A

L

V

I

L

binding phosphomethylphosphonic acid adenylate ester: V18 (≠ T14), A20 (≠ V16), N40 (= N36), G41 (= G37), G43 (= G39), K44 (= K40), S45 (= S41), T46 (= T42), Q88 (= Q82), H139 (≠ D134), M140 (≠ E135), L141 (= L136), S142 (= S137), G144 (= G139), Q145 (= Q140), Q166 (≠ E161), H198 (= H194)
binding magnesium ion: S45 (= S41), Q88 (= Q82)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenylate ester: 14

6z5uK Cryo-em structure of the a. Baumannii mlabdef complex bound to appnhp (see paper)
31% identity, 88% coverage: 1:223/252 of query aligns to 2:226/253 of 6z5uK

query
sites
6z5uK

M

L

I

I

E

E

I

V

S

K

Q

N

V

L

S

S

K

F

S

N

Y
x
R

N

G

G

E

T

R

L

V

V

I

V

Y

D

D

D

N

V

I

T

S

L

L

R

N

L

I

P

R

A

R

R

G

G

Q

I

I

T

T

S

A

I

I

I

M

G

G

P

P

N
x
S

G
|
G

A

T

G
|
G

K
|
K

S
x
T

T
|
T

L

L

S

R

M

L

V

I

S

G

R

G

L

Q

L

L

P

V

M

P

D

D

K

Q

G

G

S

E

V

V

T

L

V

L

D

D

G

G

L

K

D

D

I

I

T

A

T

Q

T

M

S

S

G

R

D

Q

V

E

L

L

-

F

-

A

A

A

R

R

-

A

R

R

L

M

S

G

I

M

L

L

R

F

Q
|
Q

E

S

N

G

A

A

I

L

N

F

S

T

R

D

L

M

T

S

V

V

R

Y

D

E

L

N

V

V

T

A

F

F

G

P

R

I

Y

R

P

A

Y

H

S

T

K

K

G

L

R

S

P

E

N

N

S

L

E

-

D

I

R

A

K

E

H

L

V

V

E

A

Q

L

A

K

I

L

G

E

Y

S

L

V

G

G

L

L

E

R

G

G

L

T

S

E

E

Q

R

L

F

M

L

P

D

T

E

E

L

L

S

S

G

G

Q

M

R

N

Q

R

R

R

A

V

F

A

V

L

A

A

M

R

V

A

L

I

C

A

Q

L

D

D

T

P

D

D

Y

L

V

I

L

M

L

Y

D

D

E
|
E

P

P

L

F

N

A

N

G

L

Q

D

D

M

P

K

I

H

V

S

K

A

G

S

V

M

L

M

T

K

R

L

L

L

I

R

R

R

S

A

L

A

R

D

E

E

A

L

L

G

D

K

L

T

T

I

T

V

I

L

I

V

V

L

S

H
|
H

D

D

I

V

N

P

F

E

A

T

S

L

C

S

Y

I

S

A

D

D

H

Y

I

I

V

Y

A

V

M

V

R

A

H

E

G

G

K

K

V

I

V

Q

H

G

Q

E

G

G

S

T

P

P

D

E

E

E

L

L

binding phosphoaminophosphonic acid-adenylate ester: R12 (≠ Y11), S37 (≠ N36), G38 (= G37), G40 (= G39), K41 (= K40), T42 (≠ S41), T43 (= T42), Q86 (= Q82), E164 (= E161), H197 (= H194)
binding magnesium ion: Q86 (= Q82), E164 (= E161)

7d0aB Acinetobacter mlafedb complex in adp-vanadate trapped vclose conformation (see paper)
31% identity, 88% coverage: 1:223/252 of query aligns to 4:228/263 of 7d0aB

query
sites
7d0aB

M

L

I

I

E

E

I

V

S

K

Q

N

V

L

S

S

K

F

S

N

Y

R

N

G

G

E

T

R

L

V

V

I

V

Y

D

D

D

N

V

I

T

S

L

L

R

N

L

I

P

R

A

R

R

G

G

Q

I

I

T

T

S

A

I

I

I

M

G

G

P

P

N
x
S

G

G

A
x
T

G
|
G

K
|
K

S

T

T

T

L

L

S

R

M

L

V

I

S

G

R

G

L

Q

L

L

P

V

M

P

D

D

K

Q

G

G

S

E

V

V

T

L

V

L

D

D

G

G

L

K

D

D

I

I

T

A

T

Q

T

M

S

S

G

R

D

Q

V

E

L

L

-

F

-

A

A

A

R

R

-

A

R

R

L

M

S

G

I

M

L

L

R

F

Q

Q

E

S

N

G

A

A

I

L

N

F

S

T

R

D

L

M

T

S

V

V

R

Y

D

E

L

N

V

V

T

A

F

F

G

P

R

I

Y

R

P

A

Y

H

S

T

K

K

G

L

R

S

P

E

N

N

S

L

E

-

D

I

R

A

K

E

H

L

V

V

E

A

Q

L

A

K

I

L

G

E

Y

S

L

V

G

G

L

L

E

R

G

G

L

T

S

E

E

Q

R

L

F

M

L

P

D

T

E
|
E

L

L

S
|
S

G

G

Q
x
M

R

N

Q

R

R

R

A

V

F

A

V

L

A

A

M

R

V

A

L

I

C

A

Q

L

D

D

T

P

D

D

Y

L

V

I

L

M

L

Y

D

D

E

E

P

P

L

F

N

A

N

G

L

Q

D

D

M

P

K

I

H

V

S

K

A

G

S

V

M

L

M

T

K

R

L

L

L

I

R

R

R

S

A

L

A

R

D

E

E

A

L

L

G

D

K

L

T

T

I

T

V

I

L

I

V

V

L

S

H

H

D

D

I

V

N

P

F

E

A

T

S

L

C

S

Y

I

S

A

D

D

H

Y

I

I

V

Y

A

V

M

V

R

A

H

E

G

G

K

K

V

I

V

Q

H

G

Q

E

G

G

S

T

P

P

D

E

E

E

L

L

binding adenosine-5'-diphosphate: S39 (≠ N36), T41 (≠ A38), G42 (= G39), K43 (= K40), E140 (= E135), S142 (= S137), M145 (≠ Q140)

7d08B Acinetobacter mlafedb complex in atp-bound vtrans1 conformation (see paper)
31% identity, 88% coverage: 1:223/252 of query aligns to 4:228/263 of 7d08B

query
sites
7d08B

M

L

I

I

E

E

I

V

S

K

Q

N

V

L

S

S

K

F

S

N

Y
x
R

N

G

G

E

T
x
R

L

V

V

I

V

Y

D

D

D

N

V

I

T

S

L

L

R

N

L

I

P

R

A

R

R

G

G

Q

I

I

T

T

S

A

I

I

I

M

G

G

P

P

N

S

G
|
G

A
x
T

G

G

K
|
K

S
x
T

T
|
T

L

L

S

R

M

L

V

I

S

G

R

G

L

Q

L

L

P

V

M

P

D

D

K

Q

G

G

S

E

V

V

T

L

V

L

D

D

G

G

L

K

D

D

I

I

T

A

T

Q

T

M

S

S

G

R

D

Q

V

E

L

L

-

F

-

A

A

A

R

R

-

A

R

R

L

M

S

G

I

M

L

L

R

F

Q

Q

E

S

N

G

A

A

I

L

N

F

S

T

R

D

L

M

T

S

V

V

R

Y

D

E

L

N

V

V

T

A

F

F

G

P

R

I

Y

R

P

A

Y

H

S

T

K

K

G

L

R

S

P

E

N

N

S

L

E

-

D

I

R

A

K

E

H

L

V

V

E

A

Q

L

A

K

I

L

G

E

Y

S

L

V

G

G

L

L

E

R

G

G

L

T

S

E

E

Q

R

L

F

M

L

P

D

T

E

E

L

L

S

S

G

G

Q

M

R

N

Q

R

R

R

A

V

F

A

V

L

A

A

M

R

V

A

L

I

C

A

Q

L

D

D

T

P

D

D

Y

L

V

I

L

M

L

Y

D

D

E
|
E

P

P

L

F

N

A

N

G

L

Q

D

D

M

P

K

I

H

V

S

K

A

G

S

V

M

L

M

T

K

R

L

L

L

I

R

R

R

S

A

L

A

R

D

E

E

A

L

L

G

D

K

L

T

T

I

T

V

I

L

I

V

V

L

S

H

H

D

D

I

V

N

P

F

E

A

T

S

L

C

S

Y

I

S

A

D

D

H

Y

I

I

V

Y

A

V

M

V

R

A

H

E

G

G

K

K

V

I

V

Q

H

G

Q

E

G

G

S

T

P

P

D

E

E

E

L

L

binding adenosine-5'-triphosphate: R14 (≠ Y11), R17 (≠ T14), G40 (= G37), T41 (≠ A38), K43 (= K40), T44 (≠ S41), T45 (= T42), E166 (= E161)

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
31% identity, 90% coverage: 1:227/252 of query aligns to 2:234/344 of 6cvlD

query
sites
6cvlD

M

M

I

I

E

K

I

L

S

S

Q

N

V

I

S

T

K

K

S

V

Y
x
F

N

H

-
x
Q

G

G

T

T

L

R

V

T

V
x
I

-

Q

-

A

-

L

D

N

V

V

T

S

L

L

R

H

L

V

P

P

A

A

R

G

G

Q

I

I

T

Y

S

G

I

V

I

I

G

G

P

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

S

R

M

C

V

V

S

N

R

L

L

L

L

E

P

R

M

P

D

T

K

E

G

G

S

S

V

V

T

L

V

V

D

D

G

G

L

Q

D

E

I

L

T

T

T

L

S

S

G

E

D

S

V

E

L

L

-

T

-

K

A

A

R

R

L

Q

-

I

-

G

S

M

I

I

L

F

R

Q

Q

H

E

F

N

N

A

L

I

L

N

S

S

S

R

R

L

-

T

-

V

-

R

-

D

-

L

-

V

T

T

V

F

F

G

G

R

N

Y

V

P

A

Y

L

S

P

-

L

-

E

-

L

K

D

G

N

R

T

P

P

N

K

S

D

E

E

D

V

R

K

K

R

H

R

V

V

E

T

Q

E

A

L

I

L

G

S

Y

L

L

V

G

G

L

L

E

G

G

D

L

K

S

H

E

D

R

S

F

Y

L

P

D

S

E
x
N

L

L

S
|
S

G

G

Q
|
Q

R

K

Q

Q

R

R

A

V

F

A

V

I

A

A

M

R

V

A

L

L

C

A

Q

S

D

N

T

P

D

K

Y

V

V

L

L

L

L

C

D

D

E

Q

P

A

L

T

N

S

N

A

L

L

D

D

M

P

K

A

H

T

S

T

A

R

S

S

M

I

M

L

K

E

L

L

R

K

R

D

A

I

A

N

D

R

E

R

L

L

G

G

K

L

T

T

I

I

V

L

L

L

V

I

L

T

H
|
H

D

E

I

M

N

D

F

V

A

V

S

K

C

R

Y

I

S

C

D

D

H

C

I

V

V

A

A

V

M

I

R

S

H

N

G

G

K

E

V

L

V

I

H

E

Q

Q

G

D

S

T

P

V

D

S

E

E

L

V

I

F

-

S

H

H

P

P

E

K

binding phosphothiophosphoric acid-adenylate ester: F12 (≠ Y11), Q14 (vs. gap), I19 (≠ V17), S41 (≠ N36), G42 (= G37), A43 (= A38), G44 (= G39), K45 (= K40), S46 (= S41), T47 (= T42), N141 (≠ E135), S143 (= S137), Q146 (= Q140), H200 (= H194)

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
31% identity, 90% coverage: 1:227/252 of query aligns to 2:234/344 of 3tuzC

query
sites
3tuzC

M

M

I

I

E

K

I

L

S

S

Q

N

V

I

S

T

K

K

S

V

Y
x
F

N

H

-
x
Q

G

G

T

T

L

R

V

T

V

I

-

Q

-

A

-

L

D

N

V

V

T

S

L

L

R

H

L

V

P

P

A

A

R

G

G

Q

I

I

T

Y

S

G

I

V

I

I

G

G

P

A

N

S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

S

R

M

C

V

V

S

N