SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_002972182.1 NCBI__GCF_000182725.1:WP_002972182.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 17 hits to proteins with known functional sites (download)

P53322 High-affinity nicotinic acid transporter; Nicotinic acid permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
26% identity, 70% coverage: 4:308/433 of query aligns to 69:383/534 of P53322

query
sites
P53322

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K283 (≠ E219) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

P0AA76 D-galactonate transporter; D-galactonate/H(+) symporter from Escherichia coli (strain K12) (see paper)
22% identity, 85% coverage: 29:396/433 of query aligns to 20:393/430 of P0AA76

query
sites
P0AA76

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Y29 (= Y38) binding D-galactonate
D31 (= D40) mutation to N: Loss of galactonate transport activity.
R32 (= R41) binding D-galactonate
Y64 (= Y73) binding D-galactonate
E118 (= E127) mutation to Q: Loss of galactonate transport activity.
W358 (= W361) binding D-galactonate

6e9nA E. Coli d-galactonate:proton symporter in the inward open form (see paper)
23% identity, 85% coverage: 29:396/433 of query aligns to 9:374/409 of 6e9nA

query
sites
6e9nA

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binding D-gluconic acid: F111 (≠ Y131), N359 (= N381)

8u3hA Structure of fmoc-leu-oh bound sialin
21% identity, 89% coverage: 34:420/433 of query aligns to 15:408/425 of 8u3hA

query
sites
8u3hA

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E

K

K

A

E

I

Y

I

I

S

L

R

S

D

S

I

L

E

R

A

-

D

N

S

Q

K

L

G

S

K

S

A

Q

A

K

S

S

H

V

S

P

L

W

M

V

S

P

L

I

V

L

K

K

N

S

P

L

M

P

L

L

W

W

V

A

M

I

T

V

L

V

I

A

Y
x
H

F

F

C

S

F
x
Y

V

N

M

W

G

T

Q

F

Y
|
Y

G

T

L

L

T

L

L

T

W

L

M

L

P

P

T

T

L

Y

I

M

R

K

A

E

S

I

G

L

V

R

T

F

S

N

N

V

V

Q

T

E

I

N

G

G

L

F

L

L

G

S

A

S

I

L

P

P

F

Y

I

L

C

G

A

S

I

W

I

L

A

C

M
|
M

V

I

I

L

F

S

S

G

R

Q

S

A

A

A

D

D

H

N

Y

L

R

R

E

A

R

-

R

K

W

W

H

N

L

F

V

S

-

T

-

L

-

C

-

V

-

R

V

I

P

F

A

S

L

L

L

I

G

G

A

M

V

I

G

G

-

P

-

A

-

V

F

F

V

L

V

V

A

A

S

G

-

F

-

I

A

G

T

C

N

D

T

Y

T

S

V

L

S

A

I

V

I

A

F

F

L

L

S

T

M

I

A

S

A

T

G

L

V

G

L

G

A

F

C

C

A
x
S

P

S

L

G

F

F

W

-

S

S

L

I

P

N

T

H

A

L

I

D

L

I

S

A

G

P

A

S

A

Y

A

A

A

G

A

I

G

L

I

L

A

G

L

I

I

T

N

N

S

T

V

F

A

A

N

T

L

I

A

P

G

G

F

M

I

V

S

G

P

P

Y

V

M

-

V

-

G

-

I

I

I

A

R

K

D

S

A

L

T

T

H

P

S

D

S

N

E

T

L

V

G

G

-

E

-

W

-

Q

-

T

-

V

M

F

Y

Y

V

I

L

A

A

A

G

A

F

I

L

N

I

V

L

F

G

G

A

A

binding leucine: M279 (= M302), S344 (≠ A357)
binding Fluorenylmethyloxycarbonyl chloride: Y19 (= Y38), H235 (≠ Y258), Y238 (≠ F261), Y243 (= Y266)

8u3gA Structure of naag-bound sialin
21% identity, 89% coverage: 34:420/433 of query aligns to 15:410/427 of 8u3gA

query
sites
8u3gA

Y
x
F

I

F

I

I

A

V

Y
|
Y

L

A

D

L

R

R

V

V

N

N

V

L

G

S

F

V

A

A

K

L

L

V

Q

D

M

M

S

V

E

D

E

S

L

T

G

G

-

K

-

Y

-

Q

F

W

S

D

E

A

A

E

I

T

Y

Q

G

G

L

W

G

I

A

L

G

G

L

S

F

F

I

Y

G

G

Y

Y

F

I

F

T

E

Q

I

I

P

P

S

G

N

G

I

Y

L

V

L

A

H

S

K

K

L

I

G

G

A

G

R

K

L

M

W

L

I

L

A

G

R

F

I

G

M

I

I

L

T

G

W

T

G

A

L

V

L

L

S

T

A

-

L

L

F

F

A

T

F

P

V

I

Q

A

T

A

E

D

W

L

-

G

-

V

-

G

Q

P

F

L

Y

I

I

V

L

L

R

R

F

A

L

L

L

E

G

G

A

L

A

G

E

E

A

G

G

V

F

T

Y
x
F

P

P

G

A

V

M

I

H

L

A

Y

M

L

W

T

S

Y

S

W

W

F

A

P

P

S

P

H

L

R

E

R

R

G

S

K

K

M

L

F

L

A

S

L

I

F

S

Q

Y

A

A

G

G

S

A

P

Q

A

L

A

G

G

T

I

V

F

I

G

S

N

L

P

P

L

L

S

S

G

G

W

I

I

I

M

-

D

-

Q

-

F

-

H

-

D

-

T

-

A

C

G

Y

W

Y

Q

M

G

N

W

W

Q

T

W

Y

M

V

F

F

V

Y

L

F

E

F

A

G

I

T

P

I

A

G

V

I

V

F

L

W

G

F

V

L

I

W

L

I

L

W

Y

L

L

V

D

S

N

D

S

T

V

P

K

Q

A

K

A

H

K

K

W

R

L

I

T

S

E

H

E

Y

E

E

K

K

A

E

I

Y

I

I

S

L

R

S

D

S

I

L

E

R

A

-

D

N

S

Q

K

L

G

S

K

S

A

Q

A

K

S

S

H

V

S

P

L

W

M

V

S

P

L

I

V

L

K

K

N

S

P

L

M

P

L

L

W

W

V

A

M

I

T

V

L

V

I

A

Y

H

F

F

C

S

F

Y

V

N

M

W

G

T

Q

F

Y

Y

G

T

L

L

T

L

L

T

W

L

M

L

P

P

T

T

L

Y

I

M

R

K

A

E

S

I

G

L

V

R

T

F

S

N

N

V

V

Q

T

E

I

N

G

G

L

F

L

L

G

S

A

S

I

L

P

P

F

Y

I

L

C

G

A

S

I

W

I

L

A

C

M

M

V

I

I

L

F

S

S

G

R

Q

S

A

A

A

D

D

H

N

Y

L

R

R

E

A

R

-

R

K

W

W

H

N

L

F

V

S

-

T

-

L

-

C

-

V

-

R

V

I

P

F

A

S

L

L

L

I

G

G

A

M

V

I

G

G

-

P

-

A

-

V

F

F

V

L

V

V

A

A

S

G

-

F

-

I

A

G

T

C

N

D

T

Y

T

S

V

L

S

A

I

V

I

A

F

F

L

L

S

T

M

I

A

S

A

T

G

L

V

G

L

G

A

F

C

C

A

S

P

S

L

G

F

F

W

-

S
|
S

L

I

P

N

T
x
H

A

L

I

D

L

I

S

A

G

P

A

S

A

Y

A

A

A

G

A

I

G

L

I
x
L

A

G

L

I

I

T

N

N

S

T

V

F

A

A

N

T

L

I

A

P

G

G

F

M

I

V

S

G

P

P

Y

V

M

-

V

-

G

-

I

I

I

A

R

K

D

S

A

L

T

T

H

P

S

D

S

N

E

T

L

V

G

G

-

E

-

W

-

Q

-

T

-

V

M

F

Y

Y

V

I

L

A

A

A

G

A

F

I

L

N

I

V

L

F

G

G

A

A

binding aspartic acid: F15 (≠ Y34), Y19 (= Y38), F118 (≠ Y131)
binding glutamic acid: S350 (= S362), H353 (≠ T365), L365 (≠ I377)

6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
22% identity, 85% coverage: 29:396/433 of query aligns to 12:358/393 of 6e9oA

query
sites
6e9oA

L

M

L

I

M

F

I

I

C

T

Y

V

I

V

I

I

A

C

Y
|
Y

L

V

D

D

R

R

V

A

N

N

V

L

G

A

F

V

A

A

K

S

L

A

Q

H

M

I

S

Q

E

E

L

F

G

G

F

I

S

T

E

K

A

A

I

E

Y

M

G

G

L

Y

G

V

A

F

G

S

L

A

F

F

F

A

I

W

G

L

Y

Y

F

T

F

L

F

C

E

Q

I

I

P

P

S

G

N

G

I

W

L

F

L

L

H

D

K

R

L

V

G

G

A

S

R

R

L

V

W

T

I

Y

A

F

R

I

I

A

M

I

I

F

T

G

W

W

G

S

L

V

L

A

S

T

A

L

L

F

F

Q

A

G

F

F

V

A

Q

T

T

G

E

L

W

M

Q

S

F

L

Y

I

I

G

L

L

R

R

F

A

L

I

L

T

G

G

A

I

A

F

E

Q

A

A

G

P

F

A

Y
x
F

P

P

G

T

V

N

I

N

L

R

Y

M

L

V

T

T

Y

S

W

W

F

F

P

P

S

E

H

H

R

E

R

R

G

A

K

S

M

A

F

V

A

G

L

F

F

Y

Q

T

A

S

G

G

S
x
Q

P

F

A

V

A

G

G

L

I

A

F

F

G

L

N

T

P

P

L

L

S

L

G

I

W

W

I

I

M

Q

D

E

Q

M

F

L

H

S

D

-

T

-

A

-

G

-

W

-

Q

-

G

-

W

W

Q

H

W

W

M

V

F

F

V

I

L

V

E

T

A

G

I

G

P

I

A

G

V

I

L

W

G

S

V

-

V

L

I

I

L

W

L

F

Y

K

L

V

D

Y

N

Q

S

P

V

P

K

R

A

L

A

T

K

K

W

G

L

I

T

S

E

K

E

A

E

E

K

-

A

-

I

-

S

-

R

L

D

D

I

Y

E

I

A

R

D

D

S

G

K

G

G

G

K

L

A

V

A

L

S

T

H

A

S

K

L

D

M

W

S

K

L

L

V

V

K

F

N

H

P

R

M

K

L

L

W

-

V

-

M

-

T

-

L

-

I

-

Y

-

F

I

C

G

F

V

V

Y

M

L

G

G

Q
|
Q

Y

F

G

A

L

V

-

A

-

S

T

T

L

L

W

W

M

F

P

F

T

L

L

T

I

L

R

K

A

A

S

-

G

-

V

-

T

-

S

-

N

-

V

-

T

-

I

-

G

G

L

F

L

M

G

T

A

T

I

V

P

P

F

F

I

L

C

A

A

A

I

F

I

V

A

G

M

V

V

L

I

L

F

S

S

G

R

W

S

V

A

A

D

D

H

L

Y

L

R

V

E

R

R

K

R

G

W

F

H

S

L

L

-

G

-

F

-

A

-

R

V

K

V

T

P

P

A

I

L

I

L

C

G

G

A

L

V

L

-

I

-

S

G

T

F

C

V

I

V

M

A

G

A

A

S

N

A

Y

T

T

N

N

T

D

T

P

V

M

S

M

I

I

I

M

F

C

L

L

S

M

M

A

A

L

A

A

A

F

G

F

V

G

L

N

A

G

C

F

A

A

P
x
S

L

I

F

T

W

W

S

S

L

L

P

V

T

S

A

S

I

L

L

A

S

P

G

M

A

R

A

L

A

I

A

G

A

L

G

T

I

G

A

G

L

V

I

F

N
|
N

S

F

V

A

A

G

N

G

L

L

A

G

G

G

F

I

I

T

S

V

P

P

Y

L

M

V

V

V

G

G

binding D-galactonic acid: Y21 (= Y38), F114 (≠ Y131), Q141 (≠ S158), Q228 (= Q265), S320 (≠ P358), N343 (= N381)

Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
24% identity, 82% coverage: 67:420/433 of query aligns to 84:454/493 of Q03567

query
sites
Q03567

G

G

L

M

F

V

F

L

I

S

G

S

Y

F

F

F

F

Y

F

G

E

Y

I

I

P

G

S

S

N

Q

I

I

L

I

L

G

H

G

K

H

L

L

G

A

A

S

R

R

L

Y

W

G

I

G

A

K

R

R

I

V

M

V

I

F

T

V

W

T

G

I

L

L

L

G

S

S

A

A

L

L

F

L

A

T

F

L

V

L

Q

N

-

P

-

V

-

A

-

R

-

T

T

S

E

E

W

Y

Q

A

F

L

Y

A

I

I

L

L

R

R

F

A

L

A

L

I

G

G

A

F

A

L

E

Q

A

G

G

A

F

T

Y

F

P

P

G

A

V

M

I

H

L

T

Y

M

L

W

T

S

Y

V

W

W

F

G

P

P

S

P

H

L

R

E

R

L

G

S

K

V

M

L

F

T

A

G

L

V

F

T

Q

Y

A

A

G

G

S

A

P

Q

A

I

A

G

G

N

I

V

F

I

G

V

N

L

P

P

L

L

S

S

G

G

W

F

I

L

M

C

D

E

Q

Y

F

G

H

F

D

D

T

-

A

-

G

-

W

-

Q

G

G

G

W

W

Q

P

W

S

M

I

F

F

V

Y

L

I

E

I

A

G

I

V

P

F

A

G

V

V

V

L

L

W

G

T

V

A

V

V

I

W

L

W

L

Y

Y

V

L

S

D

S

N

D

S

K

V

P

K

A

A

T

A

H

K

P

W

R

L

I

T

T

E

P

E

E

K

K

A

Q

I

Y

I

I

S

V

R

T

D

A

I

V

E

E

A

A

-

S

-

M

-

G

D

K

S

D

K

T

G

G

K

K

A

V

A

P

S

S

H

T

S

P

L

W

M

I

S

K

L

I

V

L

K

T

N

S

P

P

M

A

L

V

W

W

V

A

M

C

T

W

L

A

I

G

Y

H

F

F

C

A

F

G

V

D

M

W

G

G

Q

A

Y

Y

G

T

L

M

T

L

L

V

W

S

M

L

P

P

T

S

L

F

I

L

R

K

A

D

S

V

G

L

V

G

T

L

S

N

N

L

V

S

T

S

I

L

G

G

L

A

L

V

G

A

A

S

I

I

P

P

F

Y

I

I

C

A

A

Y

I

F

I

L

A

A

M

I

V

N

I

A

F

G

S

G

R

V

S

L

A

A

D

D

H

T

Y

L

R

R

E

S

-

K

-

G

-

I

-

L

-

S

-

T

-

L

-

N

-

T

R

R

W

A

H

A

L

M

V

L

V

V

P

-

A

A

L

L

L

I

G

G

A

Q

V

G

G

I

F

F

V

L

V

V

A

A

A

S

-

G

-

Y

-

C

S

G

A

C

T

G

N

Q

T

D

T

V

V

L

S

V

I

I

F

F

L

I

S

T

-

C

-

G

M

M

A

A

A

I

A

S

G

G

V

L

-

Q

-

Y

-

A

-

G

-

F

-

V

-

N

-

Y

L

L

A

E

C

I

A

A

P

P

L

P

F

F

W

-

S

-

L

-

P

-

T

-

A

-

I

-

L

-

S

S

G

G

A

T

A

V

A

M

A

G

A

T

G

G

I

-

A

-

L

-

I

-

N

N

S

T

V

I

A

S

N

A

L

L

A

A

G

G

F

I

I

I

S

S

P

P

Y

A

M

V

V

S

G

S

I

Y

I

L

R

T

D

P

A

N

T

G

H

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S

E

E

E

L

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G

Q

M

M

-

V

-

L

Y

W

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L

T

A

A

G

G

F

I

L

L

I

T

L

I

G

G

A

A

Sites not aligning to the query:

69 modified: carbohydrate, N-linked (GlcNAc...) asparagine

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
20% identity, 86% coverage: 50:420/433 of query aligns to 96:471/495 of Q9NRA2

query
sites
Q9NRA2

Q

Q

M

T

S

G

E

K

L

Y

G

Q

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A

A

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A

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K

K

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x
K

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A

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A

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L

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L

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A

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G

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I

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A

S

A

Y

A

A

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A

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N

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A

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V

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P

S

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E

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G

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-

T

-

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Y

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A

A

A

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A

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N

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G

A

A

K136 (≠ R90) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ I135) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ MF 150:151) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
IL 266:267 (≠ IS 225:226) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLR-N 268:272 (≠ RDIEAD 227:232) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (= G288) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (= P294) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
G371 (= G326) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
23% identity, 64% coverage: 118:392/433 of query aligns to 166:441/495 of Q5Q0U0

query
sites
Q5Q0U0

I

V

L

L

R
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R

F

A

L

L

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x
E

G
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G

A

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A

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E

A
x
G

G

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Y
x
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P

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x
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x
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x
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K

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M

I

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-

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A

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I

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A

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A

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A

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G

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R168 (= R120) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
E171 (≠ L123) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
G172 (= G124) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E127) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (≠ A128) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (≠ Y131) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (= P132) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ I135) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ L138) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
SSL----KN 268:272 (≠ RDIEADSKG 227:235) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
P334 (= P294) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
G371 (= G326) mutation to V: Remains in the endoplasmic reticulum.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
136 K→E: Markedly decreases H(+)-coupled sialic acid transporter activity.

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
26% identity, 46% coverage: 83:281/433 of query aligns to 80:286/452 of Q5EXK5

query
sites
Q5EXK5

L

L

L

S

H
x
D

K

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A

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K

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A

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L

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K

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A

A

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A

A

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K

A

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K

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M

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L

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L

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G

G

V

I

D82 (≠ H85) mutation to A: Loss of activity.

Sites not aligning to the query:

311 V→W: Loss of activity.
314 D→A: Loss of activity.

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
23% identity, 54% coverage: 118:351/433 of query aligns to 166:401/495 of Q8BN82

query
sites
Q8BN82

I

V

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A

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H

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K

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I

V

L

K

K

N

S

P

L

M

P

L

L

W

W

V

A

M

I

T

V

L

V

I

A

Y

H

F

F

C

S

F

Y

V

N

M

W

G

S

Q

F

Y

Y

G

T

L

L

T

L

L

T

W

L

M

L

P

P

T

T

L

Y

I

M

R

K

A

E

S

I

G

L

V

R

T

F

S

N

N

V

V

Q

T

E

I

N

G

G

L

F

L

L

G

S

A

A

I

L

P

P

F

Y

I

F

C

G

A

C

I

W

I

L

A

C

M

M

V

I

I

L

F

C

S

G

R

Q

S

A

A

A

D

D

H

Y

Y

L

R

R

E

-

R

V

R

K

W

W

H

N

L

F

V

S

-

T

-

I

-

S

-

V

-

R

V

I

P

F

A

S

L

L

L

V

G

G

A

M

V

V

G

G

-

P

-

A

-

V

F

F

V

L

V

V

A

A

S

G

-

F

-

I

A

G

T

C

N

D

T

Y

T

S

V

L

S

A

I

V

I

A

F

F

L

L

S

T

M

I

A

S

A

T

H183 (≠ I135) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q9C0U9 Uncharacterized transporter PB1C11.03 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
20% identity, 67% coverage: 44:331/433 of query aligns to 115:412/570 of Q9C0U9

query
sites
Q9C0U9

V

I

G

G

F

K

A

A

K

T

L

L

Q

S

M

Y

S

S

E

T

E

I

L

L

G

G

F

L

S

F

E

D

A

D

I

V

-

H

-

I

-

T

-

S

-

N

-

Q

Y

Y

G

N

L

N

G

L

A

N

G

T

L

L

F

F

F

Y

I

V

G

G

Y

Y

F

I

F

V

F

G

E

Q

I

F

P

P

S

G

N

H

I

Y

L

I

L

M

H

Q

K

T

L

F

G

P

A

L

R

G

L

K

W

F

I

V

A

G

R

L

I

V

M

T

I

F

T

S

W

W

G

S

L

V

L

I

S

V

A

F

L

L

F

H

A

C

F

C

V

A

Q

Y

T

N

E

Y

W

G

Q

G

F

L

Y

I

I

A

L

L

R

R

F

F

L

F

L

L

G

G

A

F

A

T

E

E

A

S

G

C

F

L

Y

L

P

P

G

A

V

M

I

E

L

A

Y

T

L

M

T

G

Y

M

W

F

F

F

P

T

S

H

H

Q

R

E

R

Q

G

A

K

F

M

L

F

Q

A

P

L

V

F

F

Q

W

A

I

G

S

S

C

P

L

A

S

A

C

G

G

I

I

F

-

G

-

N

-

P

P

L

-

S

A

G

G

W

F

I

I

M

A

D

Y

Q

G

F

L

H

E

-

F

D

V

T

T

A

K

G

S

W

I

Q

A

G

P

W

W

Q

K

W

L

M

F

F

M

V

I

L

I

E

T

A

G

I

G

P

I

A

T

V

F

L

L

G

S

V

I

V

F

I

L

L

F

Y

Y

L

Y

D

P

N

D

S

N

V

P

K

S

A

K

A

A

K

R

W

F

L

L

T

T

E

D

E

E

K

K

A

L

I

Y

I

T

S

I

R

D

D

R

I

V

E

R

A

K

D

S

S

T

K

R

G

G

K

G

A

I

A

E

S

N

-

K

-

I

-

F

-

K

H

H

S

Q

L

F

M

I

S

E

L

A

V

L

K

K

N

D

P

P

M

I

L

T

W

W

V

L

M

F

T

T

L

F

I

A

Y

A

F

F

C

T

F

L

V

M

M

L

G

S

Q

N

-

N

-

L

-

A

Y

Y

G

Q

L

Q

T

N

L

L

W

I

M

F

P

T

T

S

L

L

I

N

R

V

A

S

S

D

G

L

V

N

T

S

S

T

N

L

V

V

T

G

I

V

G

A

L

L

L

A

G

G

-

Y

-

N

A

T

I

V

P

S

F

A

I

I

C

I

A

A

I

T

I

F

A

A

M

M

V

Y

I

L

F

I

S

P

R

N

S

Q

A

S

D

-

H

A

Y

Y

R

H

E

A

R

M

R

F

W

W

H

M

L

L

V

-

P

P

A

S

L

I

L

T

G

G

A

G

V

I

G

A

F

F

V

V

Sites not aligning to the query:

14 modified: Phosphoserine

Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
22% identity, 72% coverage: 29:341/433 of query aligns to 30:354/448 of Q51955

query
sites
Q51955

L

V

M

L

I

L

C

C

Y

F

I

L

I

I

A

V

Y

F

L

L

D
|
D

R

G

V

L

N
x
D

-

T

-

A

V

M

G

G

F

F

A

I

K

A

L

P

Q

A

M

L

S

S

E

Q

E

E

L

W

G

G

F

I

S

D

E

R

A

A

I

S

Y

L

G

G

L

P

G

V

A

M

G

S

L

A

F

A

F

L

I

I

G

G

Y

M

F

V

F

F

F

G

E

A

I

L

P

G

S

S

N
x
G

I

P

L

L

L

A

H
x
D

K

R

L

F

G
|
G

A

R

R

K

L

G

W

V

I

L

A

V

R

G

I

A

M

V

I

L

T

V

W

F

G

G

L

G

L

F

S

S

A

L

L

A

F

S

A

A

F

Y

V

A

Q

T

T

N

E

V

W

D

Q

Q

F

L

Y

L

I

V

L

L

R
|
R

F

F

L

L

L

T

G

G

A

L

A

G

E

L

A

G

G

A

F

G

Y

M

P

P

G

N

V

A

I

T

L

T

Y

L

L

L

T

S

Y
x
E

W

Y

F

T

P

P

S

E

H

R

R

L

R

K

G

S

-

L

-

V

-

T

K

S

M

M

F

F

A

C

L

G

F

F

Q

N

A

L

G

G

S

M

P

A

A

G

G

G

I

F

F

-

G

-

N

-

P

-

L

I

S

S

G

A

W

K

I

M

M

I

D

P

Q

A

F

Y

H

-

D

-

T

-

A

-

G

-

W

-

Q

-

G

G

W

W

Q
x
H

W

S

M

L

F

L

V

V

L

I

E

G

A

G

I

V

P

L

A

P

V

L

L

L

G

A

V

L

V

V

I

L

L

M

L

V

Y

W

L

L

D

P

N

E

S

S

V

A

K

R

-

F

-

L

-

V

-

R

-

N

-

R

-

G

-

T

-

D

-

K

-

I

-

R

-

K

-

T

-

L

-

S

-

P

-

I

-

A

-

P

-

Q

-

V

-

A

-

E

A

A

A

G

K

S

W

F

L

S

T

V

E

P

E

E

E

Q

K

K

A

A

I

V

I

A

S

A

R

R

D

S

I

V

E

F

A

A

D

V

S

I

K

-

G

-

K

F

A

S

A

G

S

T

H

Y

S

G

L

L

M

G

S

T

L

M

V

-

K

-

N

-

P

-

M

L

L

L

W

W

V

-

M

-

T

-

L

L

I

T

Y

Y

F

F

C

M

F

G

V

L

M

V

G

I

Q

V

Y

Y

G

L

L

L

T

T

L

S

W

W

M

L

P

P

T

T

L

L

I

M

R

R

A

D

S

S

G

G

V

A

T

S

S

M

N

E

V

-

T

Q

I

A

G

A

L

F

L

I

G

G

A

A

I

L

P

F

F

Q

I

F

C

G

A

G

I

V

I

L

A

S

M

A

V

V

I

G

F

V

S

G

R

W

S

A

A

M

D
|
D

H

R

Y

Y

R

N

E

P

R
x
H

R

K

W

V

H

I

L

G

V

I

V

F

P

Y

A

L

L

L

L

A

G

G

A

V

V

F

G

A

F

Y

V

A

V

V

A

G

A

Q

S

S

A

L

T

G

N

N

T

I

T

T

V

V

D41 (= D40) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
D44 (≠ N43) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
G85 (≠ N81) mutation to V: Abolishes 4-HBA transport and chemotaxis.
D89 (≠ H85) mutation to N: Abolishes 4-HBA transport and chemotaxis.
G92 (= G88) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
R124 (= R120) mutation to A: Abolishes 4-HBA transport.
E144 (≠ Y140) mutation to A: Strong decrease in 4-HBA transport.
H183 (≠ Q186) mutation to A: Decrease in 4-HBA transport and chemotaxis.
D323 (= D310) mutation to N: Abolishes 4-HBA transport and chemotaxis.
H328 (≠ R315) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to R: Decrease in 4-HBA transport and loss of chemotaxis.

Sites not aligning to the query:

386 R→A: Strong decrease in 4-HBA transport.
398 R→A: Abolishes 4-HBA transport.
444 H→A: No change in 4-HBA transport and chemotaxis.

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
22% identity, 91% coverage: 36:429/433 of query aligns to 93:499/582 of Q9JI12

query
sites
Q9JI12

I

V

A

A

Y

I

L

V

D

D

R

M

V

V

N

N

-

N

-

S

-

T

-

I

-

H

V

R

G

G

F

G

A

K

K

V

L

I

Q

K

M

E

S

K

E

A

E

K

L

F

G

N

F

W

S

D

E

P

A

E

I

T

Y

V

G

G

L

M

G

I

A
x
H

G

G

L

S

F

F

I

W

G

G

Y

Y

F

I

F

T

E

Q

I

I

P

P

S

G

N

G

I

Y

L

I

L

A

H

S

K

R

L

L

G

A

A

A

-

N

R

R

L

V

W

F

I

G

A

A

R

A

I

I

M

L

I

L

T

T

W

S

G

T

L

L

L

N

S

M

A

L

L

I

F

P

A

S

F

A

V

A

Q

R

T

V

E

H

W

Y

Q

G

F

C

Y

V

I

I

-

F

L

V

R
|
R

F

I

L

L

L

Q

G

G

A

L

A

V

E
|
E

A

G

G

V

F

T

Y

Y

P

P

G

A

V

C

I

H

L

G

Y

I

L

W

T

S

Y

K

W

W

F

A

P

P

S

P

H

L

R

E

R

R

G

S

K

R

M

L

F

A

A

T

L

T

F

S

Q

F

A

C

G

G

S

S

P

Y

A

A

A

G

G

A

I

V

F

I

G

A

N

M

P

P

L

L

S

A

G

G

W

I

I

L

M

V

D

Q

Q

-

F

-

H

-

D

-

T

-

A

-

G

-

W

Y

Q

T

G

G

W

W

Q

S

W

S

M

V

F

F

V

Y

L

V

E

Y

A

G

I

S

P

F

A

G

V

M

V

V

L

W

G

Y

V

M

V

F

I

W

L

L

Y

V

L

S

D

Y

N

E

S

S

V

P

K

A

A

K

A

H

K

P

W

T

L

I

T

T

E

D

E

E

K

R

A

R

I

Y

I

I

S

E

R

E

D

S

I

I

E

-

A

-

D

-

S

-

K

-

G

G

K

E

A

S

A

A

S

N

-

L

H

L

S

G

L

A

M

M

S

E

L

K

V

F

K

K

N

T

P

P

-

W

-

R

-

K

-

F

-

T

-

S

-

M

M

P

L

V

W

Y

V

A

M

I

T

I

L

V

I

A

Y

N

F

F

C

C