SitesBLAST
Comparing WP_003211097.1 NCBI__GCF_000691145.1:WP_003211097.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P11959 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; EC 1.8.1.4 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
78% identity, 100% coverage: 1:469/470 of query aligns to 1:468/470 of P11959
- 39:47 (vs. 39:47, 89% identical) binding FAD
- K56 (= K56) binding FAD
- D314 (= D315) binding FAD
- A322 (= A323) binding FAD
1ebdA Dihydrolipoamide dehydrogenase complexed with the binding domain of the dihydrolipoamide acetylase (see paper)
78% identity, 97% coverage: 8:462/470 of query aligns to 2:455/455 of 1ebdA
- active site: P13 (= P19), L37 (= L43), C41 (= C47), C46 (= C52), S49 (= S55), N74 (= N80), V75 (= V81), Y180 (= Y186), E184 (= E190), S320 (= S327), H438 (= H445), H440 (= H447), E445 (= E452)
- binding flavin-adenine dinucleotide: G10 (= G16), G12 (= G18), P13 (= P19), V32 (= V38), E33 (= E39), K34 (= K40), G39 (= G45), V40 (= V46), C41 (= C47), G45 (= G51), C46 (= C52), K50 (= K56), E112 (= E118), A113 (= A119), T141 (= T147), G142 (= G148), Y180 (= Y186), I181 (= I187), R268 (= R275), D308 (= D315), A314 (≠ P321), L315 (= L322), A316 (= A323)
4jdrA Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase from escherichia coli (see paper)
48% identity, 97% coverage: 8:464/470 of query aligns to 4:461/471 of 4jdrA
- active site: P15 (= P19), L40 (= L43), C44 (= C47), C49 (= C52), S52 (= S55), E77 (≠ N80), P78 (≠ V81), I184 (≠ Y186), E188 (= E190), V324 (≠ S327), H442 (= H445), H444 (= H447), E449 (= E452)
- binding flavin-adenine dinucleotide: G12 (= G16), G14 (= G18), P15 (= P19), A16 (≠ G20), E35 (= E39), R36 (≠ K40), Y37 (vs. gap), V43 (= V46), C44 (= C47), G48 (= G51), C49 (= C52), K53 (= K56), L115 (≠ E118), G116 (≠ A119), A144 (≠ T147), G145 (= G148), I185 (= I187), G311 (= G314), D312 (= D315), M318 (≠ P321), L319 (= L322), A320 (= A323), H321 (= H324)
Sites not aligning to the query:
P0A9P0 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein; EC 1.8.1.4 from Escherichia coli (strain K12) (see 2 papers)
48% identity, 97% coverage: 8:464/470 of query aligns to 5:462/474 of P0A9P0
- K220 (≠ S221) modified: N6-acetyllysine
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
6cmzA 2.3 angstrom resolution crystal structure of dihydrolipoamide dehydrogenase from burkholderia cenocepacia in complex with fad and NAD
42% identity, 98% coverage: 9:468/470 of query aligns to 4:462/462 of 6cmzA
- active site: C42 (= C47), C47 (= C52), S50 (= S55), Y184 (= Y186), E188 (= E190), H441 (= H447), E446 (= E452)
- binding flavin-adenine dinucleotide: G11 (= G16), G13 (= G18), P14 (= P19), E34 (= E39), R35 (≠ K40), G40 (= G45), T41 (≠ V46), C42 (= C47), G46 (= G51), C47 (= C52), K51 (= K56), E116 (= E118), A117 (= A119), T145 (= T147), G146 (= G148), V180 (≠ I182), G181 (= G183), Y184 (= Y186), I185 (= I187), E204 (= E206), V268 (= V273), R270 (= R275), R273 (≠ N278), F277 (≠ L282), R289 (= R294), G308 (= G314), D309 (= D315), M315 (≠ P321), L316 (= L322), A317 (= A323), H318 (= H324)
- binding flavin mononucleotide: L152 (= L154), P153 (= P155)
6uziC Crystal structure of dihydrolipoyl dehydrogenase from elizabethkingia anophelis nuhp1
47% identity, 97% coverage: 11:468/470 of query aligns to 8:470/470 of 6uziC
- active site: C45 (= C47), C50 (= C52), S53 (= S55), V187 (≠ Y186), E191 (= E190), H448 (= H447), E453 (= E452)
- binding flavin-adenine dinucleotide: I12 (= I15), G13 (= G16), G15 (= G18), P16 (= P19), G17 (= G20), E36 (= E39), K37 (= K40), G43 (= G45), T44 (≠ V46), C45 (= C47), G49 (= G51), C50 (= C52), S53 (= S55), K54 (= K56), V117 (≠ E118), G118 (≠ A119), T147 (= T147), G148 (= G148), I188 (= I187), R276 (= R275), D316 (= D315), M322 (≠ P321), L323 (= L322), A324 (= A323)
- binding zinc ion: H448 (= H447), E453 (= E452)
6cmzB 2.3 angstrom resolution crystal structure of dihydrolipoamide dehydrogenase from burkholderia cenocepacia in complex with fad and NAD
43% identity, 97% coverage: 9:463/470 of query aligns to 4:457/459 of 6cmzB
- active site: C42 (= C47), C47 (= C52), S50 (= S55), Y184 (= Y186), E188 (= E190), H441 (= H447), E446 (= E452)
- binding adenosine-5'-diphosphate: V150 (≠ I152), L152 (= L154), G181 (= G183), G183 (= G185), A205 (≠ G207), V268 (= V273), G269 (= G274)
- binding flavin-adenine dinucleotide: G11 (= G16), G13 (= G18), P14 (= P19), E34 (= E39), R35 (≠ K40), T41 (≠ V46), C42 (= C47), G46 (= G51), C47 (= C52), K51 (= K56), E116 (= E118), A117 (= A119), T145 (= T147), G146 (= G148), S164 (= S166), Y184 (= Y186), I185 (= I187), R270 (= R275), F277 (≠ L282), G308 (= G314), D309 (= D315), M315 (≠ P321), L316 (= L322), A317 (= A323), H318 (= H324)
2eq6A Crystal structure of lipoamide dehydrogenase from thermus thermophilus hb8
47% identity, 98% coverage: 11:469/470 of query aligns to 5:459/460 of 2eq6A
- active site: V37 (≠ L43), C41 (= C47), C46 (= C52), T49 (≠ S55), A176 (≠ Y186), E180 (= E190), H435 (= H445), H437 (= H447), E442 (= E452)
- binding flavin-adenine dinucleotide: I9 (= I15), G10 (= G16), G12 (= G18), P13 (= P19), G14 (= G20), E33 (= E39), A34 (≠ K40), G39 (= G45), V40 (= V46), C41 (= C47), G45 (= G51), C46 (= C52), K50 (= K56), F111 (≠ E118), A112 (= A119), A135 (= A146), T136 (= T147), G137 (= G148), S155 (= S166), R269 (≠ N278), D306 (= D315), L312 (≠ P321), L313 (= L322), A314 (= A323), H315 (= H324), Y344 (≠ F354)
Sites not aligning to the query:
3urhB Crystal structure of a dihydrolipoamide dehydrogenase from sinorhizobium meliloti 1021
44% identity, 97% coverage: 11:467/470 of query aligns to 2:465/465 of 3urhB
- active site: Y35 (≠ L43), C39 (= C47), C44 (= C52), S47 (= S55), V183 (≠ Y186), E187 (= E190), H443 (= H445), H445 (= H447), E450 (= E452)
- binding flavin-adenine dinucleotide: I6 (= I15), G7 (= G16), G9 (= G18), P10 (= P19), G11 (= G20), E30 (= E39), K31 (= K40), G37 (= G45), T38 (≠ V46), C39 (= C47), G43 (= G51), C44 (= C52), K48 (= K56), T111 (≠ E118), G112 (≠ A119), A140 (= A146), T141 (= T147), G142 (= G148), I184 (= I187), R273 (= R275), G312 (= G314), D313 (= D315), M319 (≠ P321), L320 (= L322), A321 (= A323), H322 (= H324)
P09622 Dihydrolipoyl dehydrogenase, mitochondrial; Dihydrolipoamide dehydrogenase; Glycine cleavage system L protein; EC 1.8.1.4 from Homo sapiens (Human) (see 14 papers)
44% identity, 96% coverage: 5:456/470 of query aligns to 37:496/509 of P09622