SitesBLAST

Q7CP78 2-iminobutanoate/2-iminopropanoate deaminase; Enamine/imine deaminase; EC 3.5.99.10 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
46% identity, 98% coverage: 1:122/125 of query aligns to 1:124/128 of Q7CP78

query
sites
Q7CP78

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Y17 (= Y17) mutation to F: Deaminase activity is defective. At pH 8, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.
R105 (= R103) mutation to A: Deaminase activity is defective. At pH 8 and 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.
E120 (= E118) Important for catalytic activity at high pH; mutation to A: Deaminase activity is defective. At pH 8, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.; mutation to K: Deaminase activity is defective. At pH 8, moderately reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone. At pH 9.5, strongly reduces the increase in rate of 2-ketobutyrate formation over the rate of IlvA alone.

2b33B Crystal structure of a putative endoribonuclease (tm0215) from thermotoga maritima msb8 at 2.30 a resolution
43% identity, 98% coverage: 3:125/125 of query aligns to 2:124/126 of 2b33B

query
sites
2b33B

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binding calcium ion: D35 (= D36), E37 (= E38)

5v4dA Crystal structure of the protein of unknown function of the conserved rid protein family yyfa from yersinia pestis
50% identity, 94% coverage: 5:122/125 of query aligns to 6:124/127 of 5v4dA

query
sites
5v4dA

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binding calcium ion: D91 (≠ E90), D95 (≠ K94)

P80601 2-iminobutanoate/2-iminopropanoate deaminase; 14.3 kDa perchloric acid soluble protein; Translation inhibitor L-PSP ribonuclease; UK114 antigen; EC 3.5.99.10; EC 3.1.-.- from Capra hircus (Goat) (see paper)
44% identity, 98% coverage: 1:122/125 of query aligns to 5:126/137 of P80601

query
sites
P80601

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

8yrcA Chlorinated yabj from staphylococcus aureus (see paper)
47% identity, 98% coverage: 3:124/125 of query aligns to 3:123/125 of 8yrcA

query
sites
8yrcA

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binding chloride ion: P11 (= P11), H19 (≠ Q19), Q32 (= Q32)

P52759 2-iminobutanoate/2-iminopropanoate deaminase; Liver perchloric acid-soluble protein; L-PSP; Reactive intermediate imine deaminase A homolog; Translation inhibitor L-PSP ribonuclease; UK114 antigen homolog; rp14.5; EC 3.5.99.10 from Rattus norvegicus (Rat) (see paper)
45% identity, 98% coverage: 1:122/125 of query aligns to 5:126/137 of P52759

query
sites
P52759

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

2uynA Crystal structure of e. Coli tdcf with bound 2-ketobutyrate (see paper)
44% identity, 96% coverage: 3:122/125 of query aligns to 2:123/127 of 2uynA

query
sites
2uynA

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binding 2-ketobutyric acid: F83 (= F85), R104 (= R103), S105 (≠ A104), C106 (≠ T105)

2uykC Crystal structure of e. Coli tdcf with bound serine (see paper)
44% identity, 96% coverage: 3:122/125 of query aligns to 2:123/127 of 2uykC

query
sites
2uykC

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binding serine: R104 (= R103), S105 (≠ A104), C106 (≠ T105)

A0A1J1DL12 2-iminobutanoate/2-iminopropanoate deaminase; Allergen Der f 34; Enamine/imine deaminase; Allergen Der f 34.0101; EC 3.5.99.10 from Dermatophagoides farinae (American house dust mite) (see paper)
44% identity, 97% coverage: 3:123/125 of query aligns to 5:125/128 of A0A1J1DL12

query
sites
A0A1J1DL12

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Y19 (= Y17) mutation to A: Loss of imine deaminase activity; when associated with A-33; A-59; A-91; A-105; A-114 and A-120.
G33 (= G31) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-59; A-91; A-105; A-114 and A-120.
N59 (= N57) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-91; A-105; A-114 and A-120.
N91 (= N89) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-105; A-114 and A-120.
R105 (= R103) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-114 and A-120.
P114 (= P112) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-105 and A-120.
E120 (= E118) mutation to A: Loss of imine deaminase activity; when associated with A-19; A-33; A-59; A-91; A-105 and A-114.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q94JQ4 Reactive Intermediate Deaminase A, chloroplastic; 2-iminobutanoate/2-iminopropanoate deaminase; EC 3.5.99.10 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
44% identity, 94% coverage: 5:122/125 of query aligns to 67:184/187 of Q94JQ4

query
sites
Q94JQ4

I

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A

A

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A

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R165 (= R103) mutation to A: Loss of activity.

5hp8A Crystal structures of rida in complex with pyruvate (see paper)
43% identity, 84% coverage: 18:122/125 of query aligns to 1:105/108 of 5hp8A

query
sites
5hp8A

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binding pyruvic acid: R86 (= R103), T88 (= T105)

P0AFQ5 3-aminoacrylate deaminase RutC; 3-AA deaminase; EC 3.5.-.- from Escherichia coli (strain K12) (see paper)
29% identity, 87% coverage: 14:122/125 of query aligns to 15:123/128 of P0AFQ5

query
sites
P0AFQ5

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R104 (= R103) mutation to A: Loss of activity.

9bk8A Crystal structure of rida family protein pa5083 from pseudomonas aeruginosa with 2-ketobutyric acid bound
36% identity, 66% coverage: 26:107/125 of query aligns to 21:97/116 of 9bk8A

query
sites
9bk8A

F

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binding 2-ketobutyric acid: R93 (= R103), T94 (≠ A104), T95 (= T105)

9bk4A Crystal structure of rida family protein pa5083 from pseudomonas aeruginosa with pyruvic acid bound
36% identity, 66% coverage: 26:107/125 of query aligns to 21:97/116 of 9bk4A

query
sites
9bk4A

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binding pyruvic acid: R93 (= R103), T94 (≠ A104), T95 (= T105)

9bjyA Crystal structure of rida family protein pa5083 from pseudomonas aeruginosa with tmao bound
36% identity, 66% coverage: 26:107/125 of query aligns to 19:95/113 of 9bjyA

query
sites
9bjyA

F

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binding trimethylamine oxide: T92 (≠ A104), T93 (= T105)

3lybB Structure of putative endoribonuclease(kp1_3112) from klebsiella pneumoniae
26% identity, 86% coverage: 17:123/125 of query aligns to 1:134/137 of 3lybB

query
sites
3lybB

Y

Y

S

A

Q

A

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A

G

G

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N

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N

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binding calcium ion: N28 (vs. gap), D32 (vs. gap)

Q12429 Diphthine--ammonia ligase; Diphthamide synthase; Diphthamide synthetase; EC 6.3.1.14 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
25% identity, 93% coverage: 10:125/125 of query aligns to 442:573/685 of Q12429

query
sites
Q12429

A

A

P

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A

N

I

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A

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D

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Sites not aligning to the query:

216 G→N: Completely inactivates the enzyme.
220 mutation E->A,H: Completely inactivates the enzyme. Resistance to sordarin.

Query Sequence

>WP_003465357.1 NCBI__GCF_000359605.1:WP_003465357.1
MTKAIHTDNAPQAIGPYSQAIDAGDFVYISGQIPLDPESMQIVEGDIVAQTNQVMANLEA
ILTEAGLTFGHVVKFTIYIANMDDFATINEAYAKFLQEPYPARATVEVSKLPKGAQVEMD
AIVKK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory